MD12G1127700.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0017650
Physical Location & Seq
Reverse (-)
20295641 .. 20296890
1250 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1127700.v1.1.491

Sequence Viewer

Length: 246 bp
ATGAGGTACCATCCTGTTCCCGGTGGCCCTCCGCCTCCGCCACCATCAGACTACCCTCCCCCTCGTCCTCCTCCTGGCCCTCCACCACCTGGCTATCAGGGTTACTTTTATCCTCCCGCGCCAACCCCGAACCAGCCGCCGCCTTCTTCACTCCAAGACAATGATGGCCCAGGTTGCTGTTCAGTTCTTAGAGACTGTTTGGCTGCAATTTGTTGCTGCTGGATATTGGACTCTTGCTGCCCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CYSTM

Protein Analysis

82

Amino Acids

8.57

Weight (kDa)

4.66

Isoelectric Point (pI)

96.13

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0017650)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g23050
malus_domestica MD12G1127700.v1.1 MD16G1127000.v1.1
pyrus_communis pycom16g10780
rosa_chinensis RchiOBHm_Chr4g0429321
rosa_laevigata RLG00000007081
rosa_roxburghii Rroxscaffold_5G00370930
rosa_samantha Rh4AG290700 Rh4BG296500 Rh4CG312200 Rh4DG293600
rosa_wichuraiana Rw4G025170

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 6
AccB1I GGYRCC 1 cut(s) 6
AccB7I CCANNNNNTGG 1 cut(s) 89
AccII CGCG 1 cut(s) 119
AciI CCGC 5 cut(s) 32, 38, 117, 137, 140
AfaI GTAC 1 cut(s) 8
AfiI CCNNNNNNNGG 3 cut(s) 20, 74, 89
AjnI CCWGG 3 cut(s) 73, 88, 169
Alw26I GTCTC 1 cut(s) 186
AoxI GGCC 3 cut(s) 25, 76, 166
ApeKI GCWGC 3 cut(s) 203, 216, 237
Asp718I GGTACC 1 cut(s) 6
AspLEI GCGC 1 cut(s) 121
AspS9I GGNCC 3 cut(s) 26, 77, 167
AsuC2I CCSGG 1 cut(s) 21
BanI GGYRCC 1 cut(s) 6
BbvI GCAGC 3 cut(s) 190, 203, 224
BccI CCATC 3 cut(s) 18, 52, 158
BciT130I CCWGG 3 cut(s) 75, 90, 171
BcnI CCSGG 1 cut(s) 21
BcoDI GTCTC 1 cut(s) 186
BisI GCNGC 5 cut(s) 137, 140, 204, 217, 238
BlsI GCNGC 5 cut(s) 138, 141, 205, 218, 239
Bme1390I CCNGG 4 cut(s) 21, 75, 90, 171
BmgT120I GGNCC 3 cut(s) 26, 77, 167
BmiI GGNNCC 1 cut(s) 8
BmrFI CCNGG 4 cut(s) 21, 75, 90, 171
BpuMI CCSGG 1 cut(s) 21
BsaJI CCNNGG 1 cut(s) 169
Bsc4I CCNNNNNNNGG 3 cut(s) 20, 74, 89
BseBI CCWGG 3 cut(s) 75, 90, 171
BseDI CCNNGG 1 cut(s) 169
BseGI GGATG 1 cut(s) 10
BseLI CCNNNNNNNGG 3 cut(s) 20, 74, 89
BseRI GAGGAG 1 cut(s) 60
BseXI GCAGC 3 cut(s) 190, 203, 224
Bsh1236I CGCG 1 cut(s) 119
BshFI GGCC 3 cut(s) 27, 78, 168
BshNI GGYRCC 1 cut(s) 6
BsiSI CCGG 1 cut(s) 21
BslI CCNNNNNNNGG 3 cut(s) 20, 74, 89
BsmAI GTCTC 1 cut(s) 186
BsnI GGCC 3 cut(s) 27, 78, 168
BspACI CCGC 5 cut(s) 32, 38, 117, 137, 140
BspANI GGCC 3 cut(s) 27, 78, 168
BspFNI CGCG 1 cut(s) 119
BspLI GGNNCC 1 cut(s) 8
BspT107I GGYRCC 1 cut(s) 6
BssECI CCNNGG 1 cut(s) 169
Bst2UI CCWGG 3 cut(s) 75, 90, 171
Bst4CI ACNGT 1 cut(s) 197
BstDEI CTNAG 2 cut(s) 188, 243
BstF5I GGATG 1 cut(s) 10
BstFNI CGCG 1 cut(s) 119
BstHHI GCGC 1 cut(s) 121
BstMAI GTCTC 1 cut(s) 186
BstMWI GCNNNNNNNGC 1 cut(s) 174
BstNI CCWGG 3 cut(s) 75, 90, 171
BstSCI CCNGG 4 cut(s) 19, 73, 88, 169
BstUI CGCG 1 cut(s) 119
BstV1I GCAGC 3 cut(s) 190, 203, 224
BsuRI GGCC 3 cut(s) 27, 78, 168
BtsCI GGATG 1 cut(s) 10
CfoI GCGC 1 cut(s) 121
Cfr13I GGNCC 3 cut(s) 26, 77, 167
Csp6I GTAC 1 cut(s) 7
CviJI RGCY 6 cut(s) 27, 78, 93, 136, 168, 203
CviKI_1 RGCY 6 cut(s) 27, 78, 93, 136, 168, 203
CviQI GTAC 1 cut(s) 7
DdeI CTNAG 2 cut(s) 188, 243
EciI GGCGGA 2 cut(s) 21, 27
EcoRII CCWGG 3 cut(s) 73, 88, 169
FauI CCCGC 1 cut(s) 124
Fnu4HI GCNGC 5 cut(s) 137, 140, 204, 217, 238
Fsp4HI GCNGC 5 cut(s) 137, 140, 204, 217, 238
GlaI GCGC 1 cut(s) 120
GluI GCNGC 5 cut(s) 137, 140, 204, 217, 238
HaeIII GGCC 3 cut(s) 27, 78, 168
HapII CCGG 1 cut(s) 21
HhaI GCGC 1 cut(s) 121
Hin6I GCGC 1 cut(s) 119
HinP1I GCGC 1 cut(s) 119
HinfI GANTC 1 cut(s) 230
HpaII CCGG 1 cut(s) 21
Hpy188I TCNGA 1 cut(s) 49
HpyAV CCTTC 1 cut(s) 153
HpyCH4III ACNGT 1 cut(s) 197
HpyCH4V TGCA 1 cut(s) 206
HpyF10VI GCNNNNNNNGC 1 cut(s) 174
HpyF3I CTNAG 2 cut(s) 188, 243
HspAI GCGC 1 cut(s) 119
KpnI GGTACC 1 cut(s) 10
Lsp1109I GCAGC 3 cut(s) 190, 203, 224
MaeIII GTNAC 1 cut(s) 101
MboII GAAGA 1 cut(s) 138
MluCI AATT 1 cut(s) 207
MlyI GAGTC 1 cut(s) 224
MnlI CCTC 8 cut(s) 39, 45, 66, 72, 78, 81, 90, 123
MspI CCGG 1 cut(s) 21
MspR9I CCNGG 4 cut(s) 21, 75, 90, 171
MvaI CCWGG 3 cut(s) 75, 90, 171
MvnI CGCG 1 cut(s) 119
MwoI GCNNNNNNNGC 1 cut(s) 174
NciI CCSGG 1 cut(s) 21
NlaIV GGNNCC 1 cut(s) 8
PflMI CCANNNNNTGG 1 cut(s) 89
PkrI GCNGC 5 cut(s) 138, 141, 205, 218, 239
PleI GAGTC 1 cut(s) 224
PpsI GAGTC 1 cut(s) 224
Psp6I CCWGG 3 cut(s) 73, 88, 169
PspGI CCWGG 3 cut(s) 73, 88, 169
PspN4I GGNNCC 1 cut(s) 8
PspPI GGNCC 3 cut(s) 26, 77, 167
RsaI GTAC 1 cut(s) 8
RsaNI GTAC 1 cut(s) 7
SatI GCNGC 5 cut(s) 137, 140, 204, 217, 238
Sau96I GGNCC 3 cut(s) 26, 77, 167
SchI GAGTC 1 cut(s) 224
ScrFI CCNGG 4 cut(s) 21, 75, 90, 171
SetI ASST 3 cut(s) 8, 91, 175
Sse9I AATT 1 cut(s) 207
SsiI CCGC 5 cut(s) 32, 38, 117, 137, 140
StyD4I CCNGG 4 cut(s) 19, 73, 88, 169
TaaI ACNGT 1 cut(s) 197
TasI AATT 1 cut(s) 207
TauI GCSGC 2 cut(s) 139, 142
TseI GCWGC 3 cut(s) 203, 216, 237
Van91I CCANNNNNTGG 1 cut(s) 89
XcmI CCANNNNNNNNNTGG 1 cut(s) 161
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.