MD12G1130200.v1.1

Dirigent protein 17-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0010578
Physical Location & Seq
Forward (+)
20535403 .. 20537563
2161 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1130200.v1.1.491

Sequence Viewer

Length: 636 bp
ATGGAAAACCCCAGCAACGATCAGGAAGTAGAGCCTTCATCTGCAGCGGTTTATGAGTTACCAGGGGAGCCTGCTATTGTCATTGATGGAGTGCCCGAAATTCCCTCAACTGAAAGCACCCGTTCTGTCTCTGATGCTGCGCATGATGCAGAATCCCATGGAAATACTAACTTTGGCGAGTGGTTGGAAGGAAGAGAAGTGAGGAAGTTGTTTGGAGAGCAAAACTATATTGGCACAGTGACTGAGTATGATAAAGAAGCAGGCTGGTACAGGGTGGTGTATGAAGATGGTGACTTTGAAGATCTCGACTGGACTGAGTTGGAACAAGTGCTACTGCCCCTAGACATTATGGTTCCCCTGAAAACAGTAGCCCTAAAAACTCTCAGGAAAAGCAAGAAATTCCTCCGTAAACCTAGAAACTTTATGGCTGGATCTCAAGCACAAAAAGCCAAAACCAAGGGAAGTAGGGGAAGGAAGGCCACCTCAGCAATTGAAGGTGCTTCAAAGATTGACTTCAAAGTAGGTGAAGATGCTTCAATGGTGGACTCCAAAGTACTTGAAGATGCTTCAATGGTGGACTCCAAAGTACCTGAAGCTGCTTTAATGACAGACTCCAAAACACCTGAATATGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AT_hook LBR_tudor

Protein Analysis

212

Amino Acids

23.28

Weight (kDa)

4.71

Isoelectric Point (pI)

31.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PTM_DIR17_Tudor PF21743 64 - 111 4.4e-25 PTM/DIR17-like, Tudor domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 141
AciI CCGC 1 cut(s) 47
AclWI GGATC 1 cut(s) 439
AcsI RAATTY 2 cut(s) 99, 398
AcuI CTGAAG 1 cut(s) 612
AfaI GTAC 3 cut(s) 269, 555, 588
AgsI TTSAA 7 cut(s) 299, 494, 504, 517, 537, 560, 570
AjnI CCWGG 1 cut(s) 61
AluBI AGCT 1 cut(s) 596
AluI AGCT 1 cut(s) 596
Alw26I GTCTC 1 cut(s) 133
AlwI GGATC 1 cut(s) 439
AlwNI CAGNNNCTG 1 cut(s) 242
AoxI GGCC 1 cut(s) 477
ApeKI GCWGC 3 cut(s) 44, 137, 596
ApoI RAATTY 2 cut(s) 99, 398
AspLEI GCGC 1 cut(s) 142
AsuHPI GGTGA 2 cut(s) 302, 536
BaeGI GKGCMC 1 cut(s) 96
BbvCI CCTCAGC 1 cut(s) 484
BbvI GCAGC 3 cut(s) 56, 124, 583
BccI CCATC 2 cut(s) 80, 281
BciT130I CCWGG 1 cut(s) 63
BcoDI GTCTC 1 cut(s) 133
BfaI CTAG 2 cut(s) 341, 414
BfmI CTRYAG 1 cut(s) 42
BglII AGATCT 1 cut(s) 301
BisI GCNGC 3 cut(s) 45, 138, 597
BlsI GCNGC 3 cut(s) 46, 139, 598
BmcAI AGTACT 1 cut(s) 555
Bme1390I CCNGG 1 cut(s) 63
BmiI GGNNCC 2 cut(s) 69, 354
BmrFI CCNGG 1 cut(s) 63
BmsI GCATC 4 cut(s) 124, 136, 520, 553
Bpu10I CCTNAGC 1 cut(s) 484
BpuEI CTTGAG 1 cut(s) 420
BsaJI CCNNGG 3 cut(s) 62, 157, 456
Bse1I ACTGG 1 cut(s) 314
BseBI CCWGG 1 cut(s) 63
BseDI CCNNGG 3 cut(s) 62, 157, 456
BseMII CTCAG 4 cut(s) 234, 306, 397, 498
BseNI ACTGG 1 cut(s) 314
BseSI GKGCMC 1 cut(s) 96
BseXI GCAGC 3 cut(s) 56, 124, 583
BseYI CCCAGC 1 cut(s) 11
BshFI GGCC 1 cut(s) 479
BsmAI GTCTC 1 cut(s) 133
BsnI GGCC 1 cut(s) 479
Bsp1286I GDGCHC 1 cut(s) 96
Bsp143I GATC 3 cut(s) 19, 301, 431
Bsp19I CCATGG 1 cut(s) 157
BspACI CCGC 1 cut(s) 47
BspANI GGCC 1 cut(s) 479
BspCNI CTCAG 4 cut(s) 235, 307, 396, 497
BspLI GGNNCC 2 cut(s) 69, 354
BspMAI CTGCAG 1 cut(s) 46
BspPI GGATC 1 cut(s) 439
BsrI ACTGG 1 cut(s) 314
BssECI CCNNGG 3 cut(s) 62, 157, 456
BssMI GATC 3 cut(s) 19, 301, 431
BssT1I CCWWGG 2 cut(s) 157, 456
Bst2UI CCWGG 1 cut(s) 63
Bst4CI ACNGT 2 cut(s) 238, 367
Bst6I CTCTTC 1 cut(s) 187
BstC8I GCNNGC 2 cut(s) 72, 262
BstDEI CTNAG 4 cut(s) 243, 315, 383, 484
BstDSI CCRYGG 1 cut(s) 157
BstHHI GCGC 1 cut(s) 142
BstKTI GATC 3 cut(s) 22, 304, 434
BstMAI GTCTC 1 cut(s) 133
BstMBI GATC 3 cut(s) 19, 301, 431
BstMWI GCNNNNNNNGC 3 cut(s) 146, 446, 485
BstNI CCWGG 1 cut(s) 63
BstSCI CCNGG 1 cut(s) 61
BstSFI CTRYAG 1 cut(s) 42
BstSLI GKGCMC 1 cut(s) 96
BstV1I GCAGC 3 cut(s) 56, 124, 583
BstX2I RGATCY 2 cut(s) 301, 431
BstYI RGATCY 2 cut(s) 301, 431
BsuRI GGCC 1 cut(s) 479
BtgI CCRYGG 1 cut(s) 157
BtsIMutI CAGTG 1 cut(s) 243
Cac8I GCNNGC 2 cut(s) 72, 262
CaiI CAGNNNCTG 1 cut(s) 242
CfoI GCGC 1 cut(s) 142
Csp6I GTAC 3 cut(s) 268, 554, 587
CviAII CATG 2 cut(s) 143, 158
CviJI RGCY 8 cut(s) 34, 70, 264, 371, 428, 449, 479, 596
CviKI_1 RGCY 8 cut(s) 34, 70, 264, 371, 428, 449, 479, 596
CviQI GTAC 3 cut(s) 268, 554, 587
DdeI CTNAG 4 cut(s) 243, 315, 383, 484
DpnI GATC 3 cut(s) 21, 303, 433
DpnII GATC 3 cut(s) 19, 301, 431
Eam1104I CTCTTC 1 cut(s) 187
EarI CTCTTC 1 cut(s) 187
Eco130I CCWWGG 2 cut(s) 157, 456
Eco57I CTGAAG 1 cut(s) 612
EcoRII CCWGG 1 cut(s) 61
EcoT14I CCWWGG 2 cut(s) 157, 456
ErhI CCWWGG 2 cut(s) 157, 456
FaeI CATG 2 cut(s) 146, 161
FaiI YATR 9 cut(s) 54, 144, 159, 228, 249, 282, 350, 425, 630
FalI AAGNNNNNCTT 2 cut(s) 497, 529
FatI CATG 2 cut(s) 142, 157
Fnu4HI GCNGC 3 cut(s) 45, 138, 597
Fsp4HI GCNGC 3 cut(s) 45, 138, 597
FspBI CTAG 2 cut(s) 341, 414
FspI TGCGCA 1 cut(s) 141
GlaI GCGC 1 cut(s) 141
GluI GCNGC 3 cut(s) 45, 138, 597
GsaI CCCAGC 1 cut(s) 15
HaeIII GGCC 1 cut(s) 479
HhaI GCGC 1 cut(s) 142
Hin1II CATG 2 cut(s) 146, 161
Hin6I GCGC 1 cut(s) 140
HinP1I GCGC 1 cut(s) 140
HinfI GANTC 4 cut(s) 152, 545, 578, 611
HphI GGTGA 2 cut(s) 302, 536
Hpy166II GTNNAC 3 cut(s) 410, 544, 577
Hpy188I TCNGA 1 cut(s) 133
Hpy188III TCNNGA 3 cut(s) 23, 305, 385
Hpy8I GTNNAC 3 cut(s) 410, 544, 577
HpyAV CCTTC 5 cut(s) 45, 182, 465, 469, 488
HpyCH4III ACNGT 2 cut(s) 238, 367
HpyCH4V TGCA 2 cut(s) 44, 149
HpyF10VI GCNNNNNNNGC 3 cut(s) 146, 446, 485
HpyF3I CTNAG 4 cut(s) 243, 315, 383, 484
Hsp92II CATG 2 cut(s) 146, 161
HspAI GCGC 1 cut(s) 140
Kzo9I GATC 3 cut(s) 19, 301, 431
LmnI GCTCC 1 cut(s) 67
Lsp1109I GCAGC 3 cut(s) 56, 124, 583
LweI GCATC 4 cut(s) 124, 136, 520, 553
MaeI CTAG 2 cut(s) 341, 414
MaeIII GTNAC 3 cut(s) 57, 238, 290
MalI GATC 3 cut(s) 21, 303, 433
MboI GATC 3 cut(s) 19, 301, 431
MboII GAAGA 5 cut(s) 204, 296, 311, 539, 572
MfeI CAATTG 1 cut(s) 489
MflI RGATCY 2 cut(s) 301, 431
MhlI GDGCHC 1 cut(s) 96
MluCI AATT 3 cut(s) 99, 398, 489
MlyI GAGTC 3 cut(s) 539, 572, 605
MmeI TCCRAC 2 cut(s) 165, 300
MnlI CCTC 4 cut(s) 115, 195, 413, 493
MseI TTAA 1 cut(s) 602
MspA1I CMGCKG 1 cut(s) 47
MspR9I CCNGG 1 cut(s) 63
MunI CAATTG 1 cut(s) 489
MvaI CCWGG 1 cut(s) 63
MwoI GCNNNNNNNGC 3 cut(s) 146, 446, 485
NcoI CCATGG 1 cut(s) 157
NdeII GATC 3 cut(s) 19, 301, 431
NlaIII CATG 2 cut(s) 146, 161
NlaIV GGNNCC 2 cut(s) 69, 354
NmuCI GTSAC 2 cut(s) 238, 290
NsbI TGCGCA 1 cut(s) 141
PfeI GAWTC 1 cut(s) 152
PkrI GCNGC 3 cut(s) 46, 139, 598
PleI GAGTC 3 cut(s) 539, 572, 605
PpsI GAGTC 3 cut(s) 539, 572, 605
Psp6I CCWGG 1 cut(s) 61
PspFI CCCAGC 1 cut(s) 11
PspGI CCWGG 1 cut(s) 61
PspN4I GGNNCC 2 cut(s) 69, 354
PsrI GAACNNNNNNTAC 2 cut(s) 315, 347
PstI CTGCAG 1 cut(s) 46
PstNI CAGNNNCTG 1 cut(s) 242
PsuI RGATCY 2 cut(s) 301, 431
RsaI GTAC 3 cut(s) 269, 555, 588
RsaNI GTAC 3 cut(s) 268, 554, 587
SaqAI TTAA 1 cut(s) 602
SatI GCNGC 3 cut(s) 45, 138, 597
Sau3AI GATC 3 cut(s) 19, 301, 431
ScaI AGTACT 1 cut(s) 555
SchI GAGTC 3 cut(s) 539, 572, 605
ScrFI CCNGG 1 cut(s) 63
SduI GDGCHC 1 cut(s) 96
SetI ASST 7 cut(s) 415, 485, 499, 526, 592, 598, 625
SfaNI GCATC 4 cut(s) 124, 136, 520, 553
SfcI CTRYAG 1 cut(s) 42
SmlI CTYRAG 1 cut(s) 435
SmoI CTYRAG 1 cut(s) 435
Sse9I AATT 3 cut(s) 99, 398, 489
SsiI CCGC 1 cut(s) 47
SspMI CTAG 2 cut(s) 341, 414
StyD4I CCNGG 1 cut(s) 61
StyI CCWWGG 2 cut(s) 157, 456
TaaI ACNGT 2 cut(s) 238, 367
TaqI TCGA 1 cut(s) 306
TasI AATT 3 cut(s) 99, 398, 489
TatI WGTACW 1 cut(s) 553
TfiI GAWTC 1 cut(s) 152
Tru1I TTAA 1 cut(s) 602
Tru9I TTAA 1 cut(s) 602
TscAI CASTG 1 cut(s) 243
TseFI GTSAC 2 cut(s) 238, 290
TseI GCWGC 3 cut(s) 44, 137, 596
Tsp45I GTSAC 2 cut(s) 238, 290
TspDTI ATGAA 2 cut(s) 27, 297
TspGWI ACGGA 1 cut(s) 395
TspRI CASTG 1 cut(s) 243
XapI RAATTY 2 cut(s) 99, 398
XspI CTAG 2 cut(s) 341, 414
ZrmI AGTACT 1 cut(s) 555
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.