Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0012813

Physical Location & Seq

Genomic Coordinates Reverse (-)

20694995 .. 20698245

Gene Structure 3251 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1132000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2190 bp

ATGGAGCTCATAGATTTCAACCCGAGACCCCGAGGCTTAAACCCTACCCTCGGCGAGCTCCTCCAGTGGGTCGGAGATGCGCAGAGTCCCGACATCTCTCCTCATGATCATGTTCTTGAGTTAGGGTATGGTTGCACCTCCGGAACCTCCTCCTATCCTTTTGTCCTCTCATTCACTGATCTCACATACAGCGTAAAAGTCCAGCGCAAAATGACCTTAGTACCCGCCTGTTTCAAAGGGAAACCAAATACAGCAGAGGTTAAACTGCGCATGAACAACAACAGCACAAACGCAAACACAAAAGTTTTGCTCAATGGCATATCCGGCGAGGCCAGAGAAGGCGAGATCATGGCAGTTCTCGGAGCAAGCGGGTCAGGAAAATCAACGCTCATTGACGCTCTAGCGGATAGAATCGCCAAGGACAGCTTAAAAGGCTCGATCACACTAAACGGGGAGGCCTTAAACTCAAGGCTCTTGAAAGTCATATCAGCTTATGTCATGCAAGACGATCTTTTGTTCCCAATGCTAACGGTTGAAGAAACTCTCATGTTCTCAGCTGAATTTCGGCTTCCTCGGAGATTATCAAAGTCCAAGAAGAAAGCTCGAGTCCAGGCTCTGATCGACCAACTTGGTCTTCGAAATGCCGCCAATACTGTGATCGGTGACGAGGGCCACCGAGGTGTCTCAGGAGGTGAAAGAAGGCGGGTTTCAATTGGAATCGACATAATTCACGACCCCATTGTTCTGTTTCTCGATGAGCCGACCTCTGGGCTGGATTCGACGAGCGCTTATATGGTGGTGAAGGTTTTGCAGCGGATTGCACAGAGTGGGAGCATTGTGGTCATGTCTATTCATCAACCTAGTTACAGAATATTGAGCTTGATGGACCGGTTGATTTTCTTGTCACATGGACAGACTGTGTATAGCGGCTCGCCAGCGAATCTTCCGGTGTTTTTCGGGGAGTTCGGGCATCCGATTCCTGAGACTGAGAATCGGACTGAGTTTGCTCTTGACCTCATTCGAGAACTCGAGGAAACCCCAGGCGGAACCAAGAACTTGGTGGAATTCAACAAATCATGGCAGATCACCTTGGACCAACAAAAACAACGAGAAAATCAGAACAACTTGATCAACTCCAACTGCAAGCCAAAGCTTTCTCTCAAAGATGCAATTAGTGCTAGCATTTCAAGAGGCAAACTAGTCTCCGGAGCACCAAATGACTCAAACCTCTCCTCATCAGTCCCCACATTTGCCAACCCTTCTTGGATTGAAATTGCAGTCATCTCCAAACGCTCCCTCACAAACTCCCGAAGAATGCCCGAACTCTTCGGCATTCGCCTAGGTGCGGTCCTCGTCACCGGCATCATCCTAGCCACCATGTTTTGGCACCTAGACAATTCCCCGAAAGGCATCCAAGAGCGCTTAGGGTTCTTCGCATTCGCCATGTCTACCACATTCTACACTTGCGCCGAGGCCATCCCCGTCTTCCTCCAAGAACGCTACATTTTCATGCGTGAAACCGCCTACAATGCCTACCGCCGTTCCTCATACGTCCTAGCTCATTCCCTAATCTCCATTCCCTCCCTAGCCTTCCTCTCCATAGCCTTTGCCGCTACTACTTTCTGGGCCGTGGGGCTCGCTGGTGGGCTCCACGGCTTCCTCTTCTTCTTTTTCACTATTTTTGCGGCCTTCTGGGCTGGGAGTTCATTCGTCACGTTCCTCTCAGGCGTCGTGACACATGTCATGTTGGGATACACAGTTGTGGTGGCAGTTCTAGCTTATTTCCTACTCTTTAGTGGTTTTTTCATTAGTAGGGATAGAATCCCACCCTACTGGCTTTGGTTTCACTACATTTCCCTGGTGAAGTACCCTTATGAGGGCGTTTTGCAAAACGAACTCGATGATCCAATCAAGTGCTTCGTGAGGGGGACCCAGATGTTTGACAACTCACCAATAGAAGCCTTCCCCGTAGCAATGAAGTTGAAGCTTCTCAAGAGCATGAGCACAACTTTGGGCGTGAACATCACGGCGTCCACCTGCGTGACCACCGGAATGGATATACTGAAGCAGCAGGGGATCACGGACCTCAGTAAATGGAGTTGCCTGTGGATCACCATTGCTTGGGGGTTCTTCTTTAGGATTCTCTTTTACTTTACCTTGTTGTTGGGCAGTAAAAATAAGAGGAGGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005215 GO:0005575 GO:0005623 GO:0005886 GO:0006725 GO:0006810 GO:0008150 GO:0008152 GO:0009058 GO:0009698 GO:0009699 GO:0009987 GO:0010345 GO:0015399 GO:0015405 GO:0016020 GO:0016021 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019438 GO:0019748 GO:0022804 GO:0022857 GO:0031224 GO:0042623 GO:0042626 GO:0043492 GO:0044237 GO:0044249 GO:0044425 GO:0044464 GO:0044550 GO:0051179 GO:0051234 GO:0055085 GO:0071704 GO:0071944 GO:1901360 GO:1901362 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko01523 ko02010 ko04976 map01523 map02010 map04976

Pfam Domains

Protein Families

ABC2_membrane ABC_tran

Protein Analysis

730

Amino Acids

80.97

Weight (kDa)

9.12

Isoelectric Point (pI)

37.45

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ABC_tran	PF00005	104 - 256	4e-27	ABC transporter
ABC2_membrane	PF01061	425 - 633	3.4e-46	ABC-2 type transporter

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0012813)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G37360 AT3G53510
fragaria_vesca	FvH4_6g14770
malus_domestica	MD04G1116300.v1.1 MD12G1132000.v1.1
prunus_persica	Prupe.6G246200_v2.0.a1
pyrus_communis	pycom04g10630 pycom12g12800
rosa_chinensis	RchiOBHm_Chr3g0467991
rosa_laevigata	RLG00000024461
rosa_multiflora	Rmu_co8482947.1_g000001
rosa_roxburghii	Rroxscaffold_6G00413230
rosa_rugosa	Rorug03G0090900
rosa_samantha	Rh3AG139700 Rh3BG161200 Rh3CG161100 Rh3DG161600
rosa_wichuraiana	Rw3G013210

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	2045
Acc16I	TGCGCA	2 cut(s)	81, 269
Acc36I	ACCTGC	1 cut(s)	2045
AccB1I	GGYRCC	1 cut(s)	1386
AccB7I	CCANNNNNTGG	2 cut(s)	1383, 2121
AccI	GTMKAC	1 cut(s)	1448
AccIII	TCCGGA	2 cut(s)	140, 1205
AclWI	GGATC	3 cut(s)	1898, 2084, 2117
AcsI	RAATTY	2 cut(s)	560, 1064
AcuI	CTGAAG	1 cut(s)	2084
AcyI	GRCGYC	2 cut(s)	1728, 2030
AdeI	CACNNNGTG	1 cut(s)	827
AfaI	GTAC	2 cut(s)	222, 1868
AfeI	AGCGCT	2 cut(s)	787, 1421
AfiI	CCNNNNNNNGG	8 cut(s)	50, 67, 767, 1345, 1383, 1876, 1877, 2121
AflIII	ACRYGT	1 cut(s)	1738
AgeI	ACCGGT	1 cut(s)	888
AgsI	TTSAA	9 cut(s)	19, 235, 478, 536, 711, 1069, 1188, 1271, 1984
AhlI	ACTAGT	1 cut(s)	1198
AjnI	CCWGG	3 cut(s)	609, 1039, 1857
AjuI	GAANNNNNNNTTGG	2 cut(s)	618, 650
AleI	CACNNNNGTG	3 cut(s)	678, 1760, 2039
Alw21I	GWGCWC	4 cut(s)	9, 60, 1213, 2006
Alw26I	GTCTC	4 cut(s)	19, 688, 977, 1207
AlwI	GGATC	3 cut(s)	1898, 2084, 2117
AlwNI	CAGNNNCTG	1 cut(s)	616
Ama87I	CYCGRG	4 cut(s)	22, 30, 603, 1028
Aor13HI	TCCGGA	2 cut(s)	140, 1205
Aor51HI	AGCGCT	2 cut(s)	787, 1421
AoxI	GGCC	6 cut(s)	330, 456, 670, 1473, 1626, 1686
ApeKI	GCWGC	2 cut(s)	811, 2068
ApoI	RAATTY	2 cut(s)	560, 1064
AsiGI	ACCGGT	1 cut(s)	888
AspA2I	CCTAGG	1 cut(s)	1339
AspLEI	GCGC	6 cut(s)	82, 207, 270, 788, 1422, 1469
AspS9I	GGNCC	7 cut(s)	670, 886, 1093, 1348, 1626, 1929, 2083
AsuHPI	GGTGA	8 cut(s)	674, 704, 811, 1078, 1348, 1873, 1941, 2104
AsuII	TTCGAA	1 cut(s)	637
AsuNHI	GCTAGC	1 cut(s)	1178
AvaI	CYCGRG	4 cut(s)	22, 30, 603, 1028
AvaII	GGWCC	5 cut(s)	886, 1093, 1348, 1929, 2083
AvrII	CCTAGG	1 cut(s)	1339
BaeI	ACNNNNGTAYC	2 cut(s)	204, 237
BanI	GGYRCC	1 cut(s)	1386
BanII	GRGCYC	4 cut(s)	9, 60, 1638, 1650
BbsI	GAAGAC	2 cut(s)	626, 1477
Bbv12I	GWGCWC	4 cut(s)	9, 60, 1213, 2006
BbvI	GCAGC	2 cut(s)	823, 2080
BccI	CCATC	2 cut(s)	877, 1484
BceAI	ACGGC	4 cut(s)	1524, 1613, 1669, 2043
BciT130I	CCWGG	3 cut(s)	611, 1041, 1859
BciVI	GTATCC	1 cut(s)	1745
BclI	TGATCA	2 cut(s)	106, 1128
BcoDI	GTCTC	4 cut(s)	19, 688, 977, 1207
BcuI	ACTAGT	1 cut(s)	1198
BfoI	RGCGCY	2 cut(s)	789, 1423
BfuAI	ACCTGC	1 cut(s)	2045
BfuI	GTATCC	1 cut(s)	1745
BglI	GCCNNNNNGGC	1 cut(s)	1694
BisI	GCNGC	6 cut(s)	645, 812, 928, 1611, 1686, 2069
BlnI	CCTAGG	1 cut(s)	1339
BlsI	GCNGC	6 cut(s)	646, 813, 929, 1612, 1687, 2070
Bme1390I	CCNGG	3 cut(s)	611, 1041, 1859
Bme18I	GGWCC	5 cut(s)	886, 1093, 1348, 1929, 2083
BmeT110I	CYCGRG	4 cut(s)	22, 30, 603, 1028
BmgT120I	GGNCC	7 cut(s)	670, 886, 1093, 1348, 1626, 1929, 2083
BmiI	GGNNCC	6 cut(s)	145, 1048, 1388, 1649, 1930, 1931
BmrFI	CCNGG	3 cut(s)	611, 1041, 1859
BmsI	GCATC	5 cut(s)	67, 979, 1156, 1371, 1419
BmtI	GCTAGC	1 cut(s)	1182
BoxI	GACNNNNGTC	1 cut(s)	1739
BpiI	GAAGAC	2 cut(s)	626, 1477
BplI	GAGNNNNNCTC	2 cut(s)	749, 781
BpmI	CTGGAG	1 cut(s)	47
Bpu10I	CCTNAGC	1 cut(s)	1423
Bpu14I	TTCGAA	1 cut(s)	637
BpuEI	CTTGAG	3 cut(s)	137, 451, 1976
BsaHI	GRCGYC	2 cut(s)	1728, 2030
BsaI	GGTCTC	1 cut(s)	19
BsaWI	WCCGGW	5 cut(s)	140, 888, 946, 1205, 2048
Bsc4I	CCNNNNNNNGG	8 cut(s)	50, 67, 767, 1345, 1383, 1876, 1877, 2121
Bse118I	RCCGGY	2 cut(s)	888, 1358
Bse1I	ACTGG	2 cut(s)	64, 1838
Bse3DI	GCAATG	2 cut(s)	1980, 2115
BseAI	TCCGGA	2 cut(s)	140, 1205
BseBI	CCWGG	3 cut(s)	611, 1041, 1859
BseGI	GGATG	4 cut(s)	970, 1365, 1410, 1476
BseLI	CCNNNNNNNGG	8 cut(s)	50, 67, 767, 1345, 1383, 1876, 1877, 2121
BseMI	GCAATG	2 cut(s)	1980, 2115
BseMII	CTCAG	7 cut(s)	567, 699, 972, 978, 990, 1737, 2101
BseNI	ACTGG	2 cut(s)	64, 1838
BseRI	GAGGAG	4 cut(s)	50, 90, 139, 1222
BseXI	GCAGC	2 cut(s)	823, 2080
BseYI	CCCAGC	1 cut(s)	1697
BshFI	GGCC	6 cut(s)	332, 458, 672, 1475, 1628, 1688
BshNI	GGYRCC	1 cut(s)	1386
BshTI	ACCGGT	1 cut(s)	888
BsiHKAI	GWGCWC	4 cut(s)	9, 60, 1213, 2006
BsiHKCI	CYCGRG	4 cut(s)	22, 30, 603, 1028
BsiSI	CCGG	7 cut(s)	141, 324, 889, 947, 1206, 1359, 2049
BslFI	GGGAC	3 cut(s)	72, 1226, 1942
BslI	CCNNNNNNNGG	8 cut(s)	50, 67, 767, 1345, 1383, 1876, 1877, 2121
BsmAI	GTCTC	4 cut(s)	19, 688, 977, 1207
BsmFI	GGGAC	3 cut(s)	72, 1226, 1942
BsmI	GAATGC	3 cut(s)	1320, 1332, 1436
BsnI	GGCC	6 cut(s)	332, 458, 672, 1475, 1628, 1688
Bso31I	GGTCTC	1 cut(s)	19
BsoBI	CYCGRG	4 cut(s)	22, 30, 603, 1028
Bsp119I	TTCGAA	1 cut(s)	637
Bsp1286I	GDGCHC	6 cut(s)	9, 60, 1213, 1638, 1650, 2006
Bsp13I	TCCGGA	2 cut(s)	140, 1205
BspANI	GGCC	6 cut(s)	332, 458, 672, 1475, 1628, 1688
BspCNI	CTCAG	7 cut(s)	566, 698, 973, 979, 991, 1736, 2100
BspEI	TCCGGA	2 cut(s)	140, 1205
BspHI	TCATGA	1 cut(s)	103
BspLI	GGNNCC	6 cut(s)	145, 1048, 1388, 1649, 1930, 1931
BspMI	ACCTGC	1 cut(s)	2045
BspOI	GCTAGC	1 cut(s)	1182
BspPI	GGATC	3 cut(s)	1898, 2084, 2117
BspT104I	TTCGAA	1 cut(s)	637
BspT107I	GGYRCC	1 cut(s)	1386
BspTNI	GGTCTC	1 cut(s)	19
BsrDI	GCAATG	2 cut(s)	1980, 2115
BsrFI	RCCGGY	2 cut(s)	888, 1358
BsrI	ACTGG	2 cut(s)	64, 1838
BssAI	RCCGGY	2 cut(s)	888, 1358
BssNI	GRCGYC	2 cut(s)	1728, 2030
BssT1I	CCWWGG	3 cut(s)	417, 1089, 1339
Bst2UI	CCWGG	3 cut(s)	611, 1041, 1859
Bst4CI	ACNGT	4 cut(s)	532, 655, 919, 1759
Bst6I	CTCTTC	2 cut(s)	1331, 1667
BstACI	GRCGYC	2 cut(s)	1728, 2030
BstAPI	GCANNNNNTGC	1 cut(s)	1175
BstBI	TTCGAA	1 cut(s)	637
BstC8I	GCNNGC	7 cut(s)	56, 367, 932, 936, 1145, 1180, 1638
BstDEI	CTNAG	9 cut(s)	217, 553, 685, 981, 987, 999, 1423, 1723, 2087
BstDSI	CCRYGG	2 cut(s)	1629, 1651
BstF5I	GGATG	4 cut(s)	970, 1365, 1410, 1476
BstH2I	RGCGCY	2 cut(s)	789, 1423
BstHHI	GCGC	6 cut(s)	82, 207, 270, 788, 1422, 1469
BstMAI	GTCTC	4 cut(s)	19, 688, 977, 1207
BstMWI	GCNNNNNNNGC	7 cut(s)	324, 432, 1175, 1529, 1610, 1694, 1775
BstNI	CCWGG	3 cut(s)	611, 1041, 1859
BstNSI	RCATGY	1 cut(s)	1742
BstPAI	GACNNNNGTC	1 cut(s)	1739
BstSCI	CCNGG	3 cut(s)	609, 1039, 1857
BstV1I	GCAGC	2 cut(s)	823, 2080
BstV2I	GAAGAC	2 cut(s)	626, 1477
BstXI	CCANNNNNNTGG	3 cut(s)	1057, 1833, 2053
BsuI	GTATCC	1 cut(s)	1745
BsuRI	GGCC	6 cut(s)	332, 458, 672, 1475, 1628, 1688
BtgI	CCRYGG	2 cut(s)	1629, 1651
BtsCI	GGATG	4 cut(s)	970, 1365, 1410, 1476
BtsIMutI	CAGTG	2 cut(s)	71, 174
BveI	ACCTGC	1 cut(s)	2045
Cac8I	GCNNGC	7 cut(s)	56, 367, 932, 936, 1145, 1180, 1638
CaiI	CAGNNNCTG	1 cut(s)	616
CciI	TCATGA	1 cut(s)	103
CfoI	GCGC	6 cut(s)	82, 207, 270, 788, 1422, 1469
Cfr10I	RCCGGY	2 cut(s)	888, 1358
Cfr13I	GGNCC	7 cut(s)	670, 886, 1093, 1348, 1626, 1929, 2083
CseI	GACGC	3 cut(s)	404, 1717, 2019
Csp6I	GTAC	2 cut(s)	221, 1867
CspAI	ACCGGT	1 cut(s)	888
CviQI	GTAC	2 cut(s)	221, 1867
DdeI	CTNAG	9 cut(s)	217, 553, 685, 981, 987, 999, 1423, 1723, 2087
DraIII	CACNNNGTG	1 cut(s)	827
Eam1104I	CTCTTC	2 cut(s)	1331, 1667
EarI	CTCTTC	2 cut(s)	1331, 1667
EciI	GGCGGA	1 cut(s)	1059
Ecl136II	GAGCTC	2 cut(s)	7, 58
Eco130I	CCWWGG	3 cut(s)	417, 1089, 1339
Eco147I	AGGCCT	1 cut(s)	458
Eco24I	GRGCYC	4 cut(s)	9, 60, 1638, 1650
Eco31I	GGTCTC	1 cut(s)	19
Eco47I	GGWCC	5 cut(s)	886, 1093, 1348, 1929, 2083
Eco47III	AGCGCT	2 cut(s)	787, 1421
Eco53kI	GAGCTC	2 cut(s)	7, 58
Eco57I	CTGAAG	1 cut(s)	2084
Eco88I	CYCGRG	4 cut(s)	22, 30, 603, 1028
EcoICRI	GAGCTC	2 cut(s)	7, 58
EcoO109I	RGGNCCY	1 cut(s)	1929
EcoRI	GAATTC	1 cut(s)	1064
EcoRII	CCWGG	3 cut(s)	609, 1039, 1857
EcoT14I	CCWWGG	3 cut(s)	417, 1089, 1339
EcoT38I	GRGCYC	4 cut(s)	9, 60, 1638, 1650
ErhI	CCWWGG	3 cut(s)	417, 1089, 1339
FalI	AAGNNNNNCTT	4 cut(s)	410, 442, 495, 527
FaqI	GGGAC	3 cut(s)	72, 1226, 1942
FauI	CCCGC	3 cut(s)	232, 362, 696
FbaI	TGATCA	2 cut(s)	106, 1128
FblI	GTMKAC	1 cut(s)	1448
Fnu4HI	GCNGC	6 cut(s)	645, 812, 928, 1611, 1686, 2069
FokI	GGATG	4 cut(s)	957, 1352, 1397, 1463
FriOI	GRGCYC	4 cut(s)	9, 60, 1638, 1650
Fsp4HI	GCNGC	6 cut(s)	645, 812, 928, 1611, 1686, 2069
FspI	TGCGCA	2 cut(s)	81, 269
GlaI	GCGC	6 cut(s)	81, 206, 269, 787, 1421, 1468
GluI	GCNGC	6 cut(s)	645, 812, 928, 1611, 1686, 2069
GsaI	CCCAGC	1 cut(s)	1701
GsuI	CTGGAG	1 cut(s)	47
HaeII	RGCGCY	2 cut(s)	789, 1423
HaeIII	GGCC	6 cut(s)	332, 458, 672, 1475, 1628, 1688
HapII	CCGG	7 cut(s)	141, 324, 889, 947, 1206, 1359, 2049
HgaI	GACGC	3 cut(s)	404, 1717, 2019
HhaI	GCGC	6 cut(s)	82, 207, 270, 788, 1422, 1469
Hin1I	GRCGYC	2 cut(s)	1728, 2030
Hin6I	GCGC	6 cut(s)	80, 205, 268, 786, 1420, 1467
HinP1I	GCGC	6 cut(s)	80, 205, 268, 786, 1420, 1467
HindIII	AAGCTT	2 cut(s)	1151, 1985
HpaII	CCGG	7 cut(s)	141, 324, 889, 947, 1206, 1359, 2049
HphI	GGTGA	8 cut(s)	674, 704, 811, 1078, 1348, 1873, 1941, 2104
Hpy166II	GTNNAC	3 cut(s)	1449, 2020, 2034
Hpy188I	TCNGA	7 cut(s)	74, 362, 576, 618, 975, 996, 1119
Hpy8I	GTNNAC	3 cut(s)	1449, 2020, 2034
Hpy99I	CGWCG	2 cut(s)	784, 1733
HpyAV	CCTTC	7 cut(s)	332, 693, 796, 1269, 1600, 1699, 1972
HpyCH4III	ACNGT	4 cut(s)	532, 655, 919, 1759
HpyCH4IV	ACGT	2 cut(s)	1551, 1715
HpyCH4V	TGCA	8 cut(s)	135, 502, 811, 821, 1143, 1169, 1277, 1888
HpyF10VI	GCNNNNNNNGC	7 cut(s)	324, 432, 1175, 1529, 1610, 1694, 1775
HpyF3I	CTNAG	9 cut(s)	217, 553, 685, 981, 987, 999, 1423, 1723, 2087
HpySE526I	ACGT	2 cut(s)	1551, 1715
Hsp92I	GRCGYC	2 cut(s)	1728, 2030
HspAI	GCGC	6 cut(s)	80, 205, 268, 786, 1420, 1467
KflI	GGGWCCC	1 cut(s)	1929
Kpn2I	TCCGGA	2 cut(s)	140, 1205
Ksp22I	TGATCA	2 cut(s)	106, 1128
LmnI	GCTCC	7 cut(s)	4, 63, 362, 831, 1208, 1298, 1653
Lsp1109I	GCAGC	2 cut(s)	823, 2080
LweI	GCATC	5 cut(s)	67, 979, 1156, 1371, 1419
MaeII	ACGT	2 cut(s)	1551, 1715
MaeIII	GTNAC	7 cut(s)	662, 863, 903, 1354, 1711, 1732, 2041
MfeI	CAATTG	1 cut(s)	711
MhlI	GDGCHC	6 cut(s)	9, 60, 1213, 1638, 1650, 2006
MluCI	AATT	7 cut(s)	560, 711, 726, 1064, 1170, 1272, 1396
MlyI	GAGTC	3 cut(s)	94, 615, 1214
MmeI	TCCRAC	2 cut(s)	52, 1161
MroI	TCCGGA	2 cut(s)	140, 1205
MseI	TTAA	4 cut(s)	38, 261, 428, 461
MslI	CAYNNNNRTG	6 cut(s)	108, 678, 1508, 1760, 2039, 2051
MspA1I	CMGCKG	2 cut(s)	557, 814
MspI	CCGG	7 cut(s)	141, 324, 889, 947, 1206, 1359, 2049
MspR9I	CCNGG	3 cut(s)	611, 1041, 1859
MunI	CAATTG	1 cut(s)	711
Mva1269I	GAATGC	3 cut(s)	1320, 1332, 1436
MvaI	CCWGG	3 cut(s)	611, 1041, 1859
MwoI	GCNNNNNNNGC	7 cut(s)	324, 432, 1175, 1529, 1610, 1694, 1775
NheI	GCTAGC	1 cut(s)	1178
NlaIV	GGNNCC	6 cut(s)	145, 1048, 1388, 1649, 1930, 1931
NmeAIII	GCCGAG	2 cut(s)	30, 1495
NmuCI	GTSAC	6 cut(s)	662, 903, 1354, 1711, 1732, 2041
NsbI	TGCGCA	2 cut(s)	81, 269
NspI	RCATGY	1 cut(s)	1742
NspV	TTCGAA	1 cut(s)	637
OliI	CACNNNNGTG	3 cut(s)	678, 1760, 2039
PaeR7I	CTCGAG	2 cut(s)	603, 1028
PagI	TCATGA	1 cut(s)	103
PaqCI	CACCTGC	1 cut(s)	2045
PceI	AGGCCT	1 cut(s)	458
PciI	ACATGT	1 cut(s)	1738
PctI	GAATGC	3 cut(s)	1320, 1332, 1436
PfeI	GAWTC	8 cut(s)	411, 717, 776, 940, 976, 991, 1821, 2140
PflMI	CCANNNNNTGG	2 cut(s)	1383, 2121
PinAI	ACCGGT	1 cut(s)	888
PkrI	GCNGC	6 cut(s)	646, 813, 929, 1612, 1687, 2070
PleI	GAGTC	3 cut(s)	93, 614, 1214
PpsI	GAGTC	3 cut(s)	93, 614, 1214
PpuMI	RGGWCCY	1 cut(s)	1929
PscI	ACATGT	1 cut(s)	1738
PshAI	GACNNNNGTC	1 cut(s)	1739
Psp124BI	GAGCTC	2 cut(s)	9, 60
Psp5II	RGGWCCY	1 cut(s)	1929
Psp6I	CCWGG	3 cut(s)	609, 1039, 1857
PspFI	CCCAGC	1 cut(s)	1697
PspGI	CCWGG	3 cut(s)	609, 1039, 1857
PspN4I	GGNNCC	6 cut(s)	145, 1048, 1388, 1649, 1930, 1931
PspPI	GGNCC	7 cut(s)	670, 886, 1093, 1348, 1626, 1929, 2083
PspPPI	RGGWCCY	1 cut(s)	1929
PspXI	VCTCGAGB	2 cut(s)	603, 1028
PstNI	CAGNNNCTG	1 cut(s)	616
PvuII	CAGCTG	1 cut(s)	557
RsaI	GTAC	2 cut(s)	222, 1868
RsaNI	GTAC	2 cut(s)	221, 1867
RseI	CAYNNNNRTG	6 cut(s)	108, 678, 1508, 1760, 2039, 2051
SacI	GAGCTC	2 cut(s)	9, 60
SaqAI	TTAA	4 cut(s)	38, 261, 428, 461
SatI	GCNGC	6 cut(s)	645, 812, 928, 1611, 1686, 2069
Sau96I	GGNCC	7 cut(s)	670, 886, 1093, 1348, 1626, 1929, 2083
SchI	GAGTC	3 cut(s)	94, 615, 1214
ScrFI	CCNGG	3 cut(s)	611, 1041, 1859
SduI	GDGCHC	6 cut(s)	9, 60, 1213, 1638, 1650, 2006
SfaNI	GCATC	5 cut(s)	67, 979, 1156, 1371, 1419
Sfr274I	CTCGAG	2 cut(s)	603, 1028
SfuI	TTCGAA	1 cut(s)	637
SinI	GGWCC	5 cut(s)	886, 1093, 1348, 1929, 2083
SlaI	CTCGAG	2 cut(s)	603, 1028
SmiMI	CAYNNNNRTG	6 cut(s)	108, 678, 1508, 1760, 2039, 2051
SmlI	CTYRAG	5 cut(s)	116, 466, 603, 1028, 1991
SmoI	CTYRAG	5 cut(s)	116, 466, 603, 1028, 1991
SpeI	ACTAGT	1 cut(s)	1198
Sse9I	AATT	7 cut(s)	560, 711, 726, 1064, 1170, 1272, 1396
SseBI	AGGCCT	1 cut(s)	458
SspI	AATATT	1 cut(s)	873
SstI	GAGCTC	2 cut(s)	9, 60
StuI	AGGCCT	1 cut(s)	458
StyD4I	CCNGG	3 cut(s)	609, 1039, 1857
StyI	CCWWGG	3 cut(s)	417, 1089, 1339
TaaI	ACNGT	4 cut(s)	532, 655, 919, 1759
TaiI	ACGT	2 cut(s)	1554, 1718
TasI	AATT	7 cut(s)	560, 711, 726, 1064, 1170, 1272, 1396
TauI	GCSGC	4 cut(s)	647, 930, 1613, 1688
TfiI	GAWTC	8 cut(s)	411, 717, 776, 940, 976, 991, 1821, 2140
Tru1I	TTAA	4 cut(s)	38, 261, 428, 461
Tru9I	TTAA	4 cut(s)	38, 261, 428, 461
TscAI	CASTG	2 cut(s)	71, 181
TseFI	GTSAC	6 cut(s)	662, 903, 1354, 1711, 1732, 2041
TseI	GCWGC	2 cut(s)	811, 2068
Tsp45I	GTSAC	6 cut(s)	662, 903, 1354, 1711, 1732, 2041
TspDTI	ATGAA	6 cut(s)	287, 842, 1498, 1695, 1795, 1991
TspGWI	ACGGA	1 cut(s)	2096
TspRI	CASTG	2 cut(s)	71, 181
Van91I	CCANNNNNTGG	2 cut(s)	1383, 2121
VpaK11BI	GGWCC	5 cut(s)	886, 1093, 1348, 1929, 2083
XapI	RAATTY	2 cut(s)	560, 1064
XceI	RCATGY	1 cut(s)	1742
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1057
XhoI	CTCGAG	2 cut(s)	603, 1028
XmaJI	CCTAGG	1 cut(s)	1339
XmiI	GTMKAC	1 cut(s)	1448

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.