MD12G1144800.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0013084
Physical Location & Seq
Reverse (-)
22392827 .. 22393661
835 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1144800.v1.1.491

Sequence Viewer

Length: 381 bp
ATGGGATCAATGTCTTCACCTCTGACGATGATTTCTGCACAGCCGCTGAAACAGTCGGCAGCAGCGGTCGGTCGGAGCCCCTCCCTCTTCAGAATGCACTCTGAAAAGAAGAAATCGTTGACGGTGGTTGCTGCCATTGGAGATGTATCAGCTGATGGAACGCCCTACCTGATTGCCGGTGCTGCTGCTGTTGCTTTGCTTGGAACTGCATTTCCAATCCTCTTCTCGCGGAAGGACCTGTGTCCAGAATGCGACGGGGCAGGTTTCGTTCGGAAGGGAGGGGCAGCTTTGAGAGCAAATGCAGCGAGGAAGGACGAGGCTCAGATCGTCTGTGCTCGTTGCAATGGACTTGGCAAGCTCAACCAAGTCGACAAAAGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

127

Amino Acids

13.02

Weight (kDa)

9.74

Isoelectric Point (pI)

45.16

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013084)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G02160
fragaria_vesca FvH4_6g13360
malus_domestica MD04G1131700.v1.1 MD12G1144800.v1.1
prunus_persica Prupe.6G258300_v2.0.a1
pyrus_communis pycom04g11980 pycom12g13920
rosa_chinensis RchiOBHm_Chr3g0466241
rosa_laevigata RLG00000024594
rosa_multiflora Rmu_sc0000350.1_g000013 Rmu_sc0002249.1_g000002 Rmu_sc0017699.1_g000001 Rmu_sc0022084.1_g000005
rosa_roxburghii Rroxscaffold_6G00414700
rosa_rugosa Rorug03G0080600
rosa_samantha Rh3BG147900 Rh3DG148600
rosa_wichuraiana Rw3G011950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 251
AccI GTMKAC 1 cut(s) 369
AccII CGCG 1 cut(s) 229
AciI CCGC 3 cut(s) 44, 65, 229
AclWI GGATC 1 cut(s) 13
AcuI CTGAAG 1 cut(s) 73
AloI GAACNNNNNNTCC 2 cut(s) 196, 228
AluBI AGCT 3 cut(s) 152, 287, 358
AluI AGCT 3 cut(s) 152, 287, 358
Alw21I GWGCWC 1 cut(s) 337
AlwI GGATC 1 cut(s) 13
AlwNI CAGNNNCTG 1 cut(s) 46
ApeKI GCWGC 7 cut(s) 59, 62, 131, 182, 185, 284, 302
AspS9I GGNCC 1 cut(s) 235
AsuHPI GGTGA 1 cut(s) 9
AvaII GGWCC 1 cut(s) 235
BanII GRGCYC 1 cut(s) 80
BbsI GAAGAC 1 cut(s) 6
Bbv12I GWGCWC 1 cut(s) 337
BbvI GCAGC 7 cut(s) 71, 74, 118, 169, 172, 296, 314
BccI CCATC 1 cut(s) 149
BfuAI ACCTGC 1 cut(s) 251
BisI GCNGC 8 cut(s) 44, 60, 63, 132, 183, 186, 285, 303
BlsI GCNGC 8 cut(s) 45, 61, 64, 133, 184, 187, 286, 304
Bme18I GGWCC 1 cut(s) 235
BmgT120I GGNCC 1 cut(s) 235
BmiI GGNNCC 1 cut(s) 77
BoxI GACNNNNGTC 1 cut(s) 240
BpiI GAAGAC 1 cut(s) 6
Bse118I RCCGGY 1 cut(s) 176
Bse3DI GCAATG 1 cut(s) 349
BseMI GCAATG 1 cut(s) 349
BseMII CTCAG 1 cut(s) 335
BseXI GCAGC 7 cut(s) 71, 74, 118, 169, 172, 296, 314
BsgI GTGCAG 1 cut(s) 21
Bsh1236I CGCG 1 cut(s) 229
Bsh1285I CGRYCG 2 cut(s) 69, 73
BsiEI CGRYCG 2 cut(s) 69, 73
BsiHKAI GWGCWC 1 cut(s) 337
BsiSI CCGG 1 cut(s) 177
BsmI GAATGC 2 cut(s) 99, 254
Bsp1286I GDGCHC 2 cut(s) 80, 337
Bsp143I GATC 2 cut(s) 5, 324
BspACI CCGC 3 cut(s) 44, 65, 229
BspCNI CTCAG 1 cut(s) 334
BspFNI CGCG 1 cut(s) 229
BspLI GGNNCC 1 cut(s) 77
BspMI ACCTGC 1 cut(s) 251
BspPI GGATC 1 cut(s) 13
BsrDI GCAATG 1 cut(s) 349
BsrFI RCCGGY 1 cut(s) 176
BssAI RCCGGY 1 cut(s) 176
BssMI GATC 2 cut(s) 5, 324
Bst4CI ACNGT 2 cut(s) 54, 124
Bst6I CTCTTC 2 cut(s) 92, 227
BstC8I GCNNGC 1 cut(s) 356
BstDEI CTNAG 1 cut(s) 321
BstFNI CGCG 1 cut(s) 229
BstKTI GATC 2 cut(s) 8, 327
BstMBI GATC 2 cut(s) 5, 324
BstMCI CGRYCG 2 cut(s) 69, 73
BstMWI GCNNNNNNNGC 4 cut(s) 182, 191, 293, 302
BstPAI GACNNNNGTC 1 cut(s) 240
BstUI CGCG 1 cut(s) 229
BstV1I GCAGC 7 cut(s) 71, 74, 118, 169, 172, 296, 314
BstV2I GAAGAC 1 cut(s) 6
BveI ACCTGC 1 cut(s) 251
Cac8I GCNNGC 1 cut(s) 356
CaiI CAGNNNCTG 1 cut(s) 46
Cfr10I RCCGGY 1 cut(s) 176
Cfr13I GGNCC 1 cut(s) 235
CviJI RGCY 6 cut(s) 43, 78, 152, 287, 320, 358
CviKI_1 RGCY 6 cut(s) 43, 78, 152, 287, 320, 358
DdeI CTNAG 1 cut(s) 321
DpnI GATC 2 cut(s) 7, 326
DpnII GATC 2 cut(s) 5, 324
Eam1104I CTCTTC 2 cut(s) 92, 227
EarI CTCTTC 2 cut(s) 92, 227
Eco24I GRGCYC 1 cut(s) 80
Eco47I GGWCC 1 cut(s) 235
Eco57I CTGAAG 1 cut(s) 73
EcoO109I RGGNCCY 1 cut(s) 235
EcoT38I GRGCYC 1 cut(s) 80
FblI GTMKAC 1 cut(s) 369
Fnu4HI GCNGC 8 cut(s) 44, 60, 63, 132, 183, 186, 285, 303
FriOI GRGCYC 1 cut(s) 80
Fsp4HI GCNGC 8 cut(s) 44, 60, 63, 132, 183, 186, 285, 303
GluI GCNGC 8 cut(s) 44, 60, 63, 132, 183, 186, 285, 303
HapII CCGG 1 cut(s) 177
HincII GTYRAC 2 cut(s) 120, 370
HindII GTYRAC 2 cut(s) 120, 370
HpaII CCGG 1 cut(s) 177
HphI GGTGA 1 cut(s) 9
Hpy166II GTNNAC 2 cut(s) 120, 370
Hpy188I TCNGA 6 cut(s) 24, 75, 92, 103, 273, 324
Hpy188III TCNNGA 1 cut(s) 245
Hpy8I GTNNAC 2 cut(s) 120, 370
Hpy99I CGWCG 1 cut(s) 257
HpyAV CCTTC 3 cut(s) 226, 268, 304
HpyCH4III ACNGT 2 cut(s) 54, 124
HpyCH4V TGCA 5 cut(s) 38, 97, 209, 302, 342
HpyF10VI GCNNNNNNNGC 4 cut(s) 182, 191, 293, 302
HpyF3I CTNAG 1 cut(s) 321
Kzo9I GATC 2 cut(s) 5, 324
LmnI GCTCC 1 cut(s) 75
LpnPI CCDG 5 cut(s) 182, 190, 246, 251, 258
Lsp1109I GCAGC 7 cut(s) 71, 74, 118, 169, 172, 296, 314
MalI GATC 2 cut(s) 7, 326
MboI GATC 2 cut(s) 5, 324
MboII GAAGA 4 cut(s) 6, 79, 121, 214
MhlI GDGCHC 2 cut(s) 80, 337
MmeI TCCRAC 1 cut(s) 53
MnlI CCTC 7 cut(s) 30, 91, 95, 230, 272, 300, 310
MspA1I CMGCKG 3 cut(s) 46, 65, 152
MspI CCGG 1 cut(s) 177
Mva1269I GAATGC 2 cut(s) 99, 254
MvnI CGCG 1 cut(s) 229
MwoI GCNNNNNNNGC 4 cut(s) 182, 191, 293, 302
NdeII GATC 2 cut(s) 5, 324
NlaIV GGNNCC 1 cut(s) 77
PctI GAATGC 2 cut(s) 99, 254
PkrI GCNGC 8 cut(s) 45, 61, 64, 133, 184, 187, 286, 304
PpuMI RGGWCCY 1 cut(s) 235
PshAI GACNNNNGTC 1 cut(s) 240
Psp5II RGGWCCY 1 cut(s) 235
PspN4I GGNNCC 1 cut(s) 77
PspPI GGNCC 1 cut(s) 235
PspPPI RGGWCCY 1 cut(s) 235
PstNI CAGNNNCTG 1 cut(s) 46
PvuII CAGCTG 1 cut(s) 152
SalI GTCGAC 1 cut(s) 368
SatI GCNGC 8 cut(s) 44, 60, 63, 132, 183, 186, 285, 303
Sau3AI GATC 2 cut(s) 5, 324
Sau96I GGNCC 1 cut(s) 235
SduI GDGCHC 2 cut(s) 80, 337
SetI ASST 7 cut(s) 22, 154, 171, 240, 265, 289, 360
SinI GGWCC 1 cut(s) 235
SsiI CCGC 3 cut(s) 44, 65, 229
TaaI ACNGT 2 cut(s) 54, 124
TaqI TCGA 1 cut(s) 369
TaqII GACCGA 1 cut(s) 59
TauI GCSGC 1 cut(s) 46
TseI GCWGC 7 cut(s) 59, 62, 131, 182, 185, 284, 302
VpaK11BI GGWCC 1 cut(s) 235
XmiI GTMKAC 1 cut(s) 369
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.