Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0019282

Physical Location & Seq

Genomic Coordinates Reverse (-)

22516138 .. 22517109

Gene Structure 972 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1146400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 972 bp

ATGGGCCTCAAAGGTTTAAATACAAAGAGTTATATATTGCCACAAAAGAGTTATATATTGCCCACCTCTGGGATTGAGATTGCTGTAAAGAGGGTCTCACACGAATCAAGACAGGGGATGAAGGAGTTTGTTGCAGAAATTGTCAGCAATGGACATCTTCGTCACCAAAATTTAGTCCCATTATTGGGCTACTGCAGGAGAAAAGGTGAGCTATTGTTGGTCTATGAGTACATGCCTAATGGAAGCCAGGACAAGTACCTTTTTGGCCAACCGGCGGTCACTCTCAATTGGAGGCAAAGATTTAGAGTCATAAGAGGTGTAGCATCAGGGTTGCTTTATTTGCACGAAGAATGGGATCAGGTTGTGGTTCACAGAGATGTCAAGGCCAGCAATGTATTACTGGATGGGGAATGGAACGGAAGACTAGGAGATTTTGGTCTTACAAGATTATATGACCACGGTTCAGACCCTCAAACTACTCATATAGCTGGTACCTTTGGGTATCTAGCTCCTAAGCATACAAGATCAGGCCAGGCCACAACAAGAAGTGATGTGTTTGCTTTCGGTGTATTTTTGCTTGACGTTGCAAGTGGAAAAAGGCCAATAAAGTATCACGGCTCTGAGGATGTGCTTTTGGTTGATTTGGTCTTTTCTTGTTGGAAAGGAAATAATATTCTTGAGGCGAAAGACCCAAAGTTAGGTAATGATTTTGTAGTGGAGGAGGTGGAGTTGGTATTGAAGCTTGGGCTGTTGTGCTACCAATCAGAGCCTACAGTAAAGCCAAGGATGCGCCAAGTTGTGCAGCATTTGGAGGGTGATATTCCTTTGCCTGCAGAGTTATCACTTCTTAGTGTTTCTGTAAGTGGCTTATCCATTTCTCAGAATGAAGGTTTTGACGAGTATACAATGGCCTGGGGGTCTTCACCGTCATCATATGCTGCAGATTCGTCATTCCTCTACAGCGGTCGATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0005575 GO:0005623 GO:0005886 GO:0006950 GO:0006952 GO:0007275 GO:0008150 GO:0009555 GO:0009605 GO:0009607 GO:0009617 GO:0009719 GO:0009725 GO:0009751 GO:0010033 GO:0014070 GO:0016020 GO:0032501 GO:0032502 GO:0042221 GO:0042493 GO:0042742 GO:0043207 GO:0044464 GO:0046677 GO:0048229 GO:0048856 GO:0050896 GO:0051704 GO:0051707 GO:0071944 GO:0098542 GO:1901700

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Lectin_legB Pkinase Pkinase_Tyr

Protein Analysis

324

Amino Acids

36.05

Weight (kDa)

6.8

Isoelectric Point (pI)

41.0

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PK_Tyr_Ser-Thr	PF07714	23 - 210	1.1e-33	Protein tyrosine and serine/threonine kinase
Pkinase	PF00069	23 - 208	7.7e-34	Protein kinase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0019282)

Species	Orthologous Gene IDs
malus_domestica	MD12G1146400.v1.1
rosa_chinensis	RchiOBHm_Chr6g0253591 RchiOBHm_Chr7g0189641
rosa_multiflora	Rmu_co7977474.1_g000001 Rmu_sc0000362.1_g000011 Rmu_sc0020980.1_g000001
rosa_samantha	Rh3CG145200 Rh7DG107000

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	159
Acc65I	GGTACC	1 cut(s)	491
AccB1I	GGYRCC	1 cut(s)	491
AccI	GTMKAC	1 cut(s)	902
AciI	CCGC	2 cut(s)	275, 963
AclWI	GGATC	1 cut(s)	363
AcoI	YGGCCR	1 cut(s)	265
AcsI	RAATTY	1 cut(s)	169
AfaI	GTAC	3 cut(s)	230, 257, 493
AfiI	CCNNNNNNNGG	6 cut(s)	68, 69, 184, 185, 274, 698
AgsI	TTSAA	1 cut(s)	739
AjnI	CCWGG	3 cut(s)	246, 531, 911
AluBI	AGCT	4 cut(s)	211, 488, 509, 742
AluI	AGCT	4 cut(s)	211, 488, 509, 742
Alw26I	GTCTC	1 cut(s)	100
AlwI	GGATC	1 cut(s)	363
AoxI	GGCC	7 cut(s)	4, 265, 384, 529, 534, 599, 909
ApeKI	GCWGC	2 cut(s)	802, 938
ApoI	RAATTY	1 cut(s)	169
Asp718I	GGTACC	1 cut(s)	491
AspLEI	GCGC	1 cut(s)	792
AspS9I	GGNCC	1 cut(s)	4
AsuHPI	GGTGA	4 cut(s)	155, 218, 827, 915
BalI	TGGCCA	1 cut(s)	267
BanI	GGYRCC	1 cut(s)	491
BbsI	GAAGAC	2 cut(s)	427, 912
BbvI	GCAGC	2 cut(s)	814, 925
BccI	CCATC	1 cut(s)	398
BceAI	ACGGC	1 cut(s)	631
BciT130I	CCWGG	3 cut(s)	248, 533, 913
BcoDI	GTCTC	1 cut(s)	100
BfaI	CTAG	2 cut(s)	425, 506
BfmI	CTRYAG	5 cut(s)	193, 771, 831, 939, 958
BisI	GCNGC	2 cut(s)	803, 939
BlsI	GCNGC	2 cut(s)	804, 940
Bme1390I	CCNGG	3 cut(s)	248, 533, 913
BmgT120I	GGNCC	1 cut(s)	4
BmiI	GGNNCC	1 cut(s)	493
BmrFI	CCNGG	3 cut(s)	248, 533, 913
BmsI	GCATC	2 cut(s)	332, 777
BpiI	GAAGAC	2 cut(s)	427, 912
Bpu10I	CCTNAGC	1 cut(s)	513
BpuEI	CTTGAG	1 cut(s)	698
BsaI	GGTCTC	1 cut(s)	100
BsaJI	CCNNGG	3 cut(s)	457, 782, 912
BsaXI	ACNNNNNCTCC	4 cut(s)	190, 220, 713, 743
Bsc4I	CCNNNNNNNGG	6 cut(s)	68, 69, 184, 185, 274, 698
Bse118I	RCCGGY	1 cut(s)	271
Bse1I	ACTGG	1 cut(s)	405
Bse3DI	GCAATG	2 cut(s)	154, 397
BseBI	CCWGG	3 cut(s)	248, 533, 913
BseDI	CCNNGG	3 cut(s)	457, 782, 912
BseGI	GGATG	4 cut(s)	123, 409, 631, 792
BseLI	CCNNNNNNNGG	6 cut(s)	68, 69, 184, 185, 274, 698
BseMI	GCAATG	2 cut(s)	154, 397
BseMII	CTCAG	2 cut(s)	612, 893
BseNI	ACTGG	1 cut(s)	405
BseRI	GAGGAG	1 cut(s)	734
BseXI	GCAGC	2 cut(s)	814, 925
BsgI	GTGCAG	1 cut(s)	821
Bsh1285I	CGRYCG	1 cut(s)	967
BshFI	GGCC	7 cut(s)	6, 267, 386, 531, 536, 601, 911
BshNI	GGYRCC	1 cut(s)	491
BsiEI	CGRYCG	1 cut(s)	967
BsiSI	CCGG	1 cut(s)	272
BslFI	GGGAC	1 cut(s)	161
BslI	CCNNNNNNNGG	6 cut(s)	68, 69, 184, 185, 274, 698
BsmAI	GTCTC	1 cut(s)	100
BsmFI	GGGAC	1 cut(s)	161
BsnI	GGCC	7 cut(s)	6, 267, 386, 531, 536, 601, 911
Bso31I	GGTCTC	1 cut(s)	100
Bsp143I	GATC	2 cut(s)	355, 524
BspACI	CCGC	2 cut(s)	275, 963
BspANI	GGCC	7 cut(s)	6, 267, 386, 531, 536, 601, 911
BspCNI	CTCAG	2 cut(s)	613, 892
BspLI	GGNNCC	1 cut(s)	493
BspMAI	CTGCAG	3 cut(s)	197, 835, 943
BspPI	GGATC	1 cut(s)	363
BspT107I	GGYRCC	1 cut(s)	491
BspTNI	GGTCTC	1 cut(s)	100
BsrDI	GCAATG	2 cut(s)	154, 397
BsrFI	RCCGGY	1 cut(s)	271
BsrI	ACTGG	1 cut(s)	405
BssAI	RCCGGY	1 cut(s)	271
BssECI	CCNNGG	3 cut(s)	457, 782, 912
BssMI	GATC	2 cut(s)	355, 524
BssNAI	GTATAC	1 cut(s)	903
BssT1I	CCWWGG	1 cut(s)	782
Bst1107I	GTATAC	1 cut(s)	903
Bst2UI	CCWGG	3 cut(s)	248, 533, 913
Bst4CI	ACNGT	3 cut(s)	461, 775, 927
BstC8I	GCNNGC	2 cut(s)	388, 831
BstDEI	CTNAG	4 cut(s)	513, 621, 848, 879
BstDSI	CCRYGG	1 cut(s)	457
BstF5I	GGATG	4 cut(s)	123, 409, 631, 792
BstHHI	GCGC	1 cut(s)	792
BstKTI	GATC	2 cut(s)	358, 527
BstMAI	GTCTC	1 cut(s)	100
BstMBI	GATC	2 cut(s)	355, 524
BstMCI	CGRYCG	1 cut(s)	967
BstMWI	GCNNNNNNNGC	2 cut(s)	340, 787
BstNI	CCWGG	3 cut(s)	248, 533, 913
BstNSI	RCATGY	1 cut(s)	235
BstSCI	CCNGG	3 cut(s)	246, 531, 911
BstSFI	CTRYAG	5 cut(s)	193, 771, 831, 939, 958
BstV1I	GCAGC	2 cut(s)	814, 925
BstV2I	GAAGAC	2 cut(s)	427, 912
BstZ17I	GTATAC	1 cut(s)	903
BsuRI	GGCC	7 cut(s)	6, 267, 386, 531, 536, 601, 911
BtgI	CCRYGG	1 cut(s)	457
BtsCI	GGATG	4 cut(s)	123, 409, 631, 792
Cac8I	GCNNGC	2 cut(s)	388, 831
CfoI	GCGC	1 cut(s)	792
Cfr10I	RCCGGY	1 cut(s)	271
Cfr13I	GGNCC	1 cut(s)	4
Csp6I	GTAC	3 cut(s)	229, 256, 492
CviAII	CATG	1 cut(s)	232
CviQI	GTAC	3 cut(s)	229, 256, 492
DdeI	CTNAG	4 cut(s)	513, 621, 848, 879
DpnI	GATC	2 cut(s)	357, 526
DpnII	GATC	2 cut(s)	355, 524
DraI	TTTAAA	1 cut(s)	18
DrdI	GACNNNNNNGTC	1 cut(s)	159
DseDI	GACNNNNNNGTC	1 cut(s)	159
EaeI	YGGCCR	1 cut(s)	265
Eco130I	CCWWGG	1 cut(s)	782
Eco31I	GGTCTC	1 cut(s)	100
EcoRII	CCWGG	3 cut(s)	246, 531, 911
EcoT14I	CCWWGG	1 cut(s)	782
ErhI	CCWWGG	1 cut(s)	782
FaeI	CATG	1 cut(s)	235
FaqI	GGGAC	1 cut(s)	161
FatI	CATG	1 cut(s)	231
FauNDI	CATATG	1 cut(s)	934
FblI	GTMKAC	1 cut(s)	902
Fnu4HI	GCNGC	2 cut(s)	803, 939
FokI	GGATG	4 cut(s)	130, 416, 638, 799
Fsp4HI	GCNGC	2 cut(s)	803, 939
FspBI	CTAG	2 cut(s)	425, 506
GlaI	GCGC	1 cut(s)	791
GluI	GCNGC	2 cut(s)	803, 939
HaeIII	GGCC	7 cut(s)	6, 267, 386, 531, 536, 601, 911
HapII	CCGG	1 cut(s)	272
HhaI	GCGC	1 cut(s)	792
Hin1II	CATG	1 cut(s)	235
Hin6I	GCGC	1 cut(s)	790
HinP1I	GCGC	1 cut(s)	790
HindIII	AAGCTT	1 cut(s)	740
HinfI	GANTC	3 cut(s)	104, 306, 944
HpaII	CCGG	1 cut(s)	272
HphI	GGTGA	4 cut(s)	155, 218, 827, 915
Hpy166II	GTNNAC	2 cut(s)	370, 903
Hpy188I	TCNGA	4 cut(s)	466, 622, 766, 882
Hpy188III	TCNNGA	2 cut(s)	108, 677
Hpy8I	GTNNAC	2 cut(s)	370, 903
HpyAV	CCTTC	2 cut(s)	115, 881
HpyCH4III	ACNGT	3 cut(s)	461, 775, 927
HpyCH4IV	ACGT	1 cut(s)	582
HpyCH4V	TGCA	7 cut(s)	134, 195, 343, 587, 802, 833, 941
HpyF10VI	GCNNNNNNNGC	2 cut(s)	340, 787
HpyF3I	CTNAG	4 cut(s)	513, 621, 848, 879
HpySE526I	ACGT	1 cut(s)	582
Hsp92II	CATG	1 cut(s)	235
HspAI	GCGC	1 cut(s)	790
KpnI	GGTACC	1 cut(s)	495
Kzo9I	GATC	2 cut(s)	355, 524
LmnI	GCTCC	1 cut(s)	514
Lsp1109I	GCAGC	2 cut(s)	814, 925
LweI	GCATC	2 cut(s)	332, 777
MaeI	CTAG	2 cut(s)	425, 506
MaeII	ACGT	1 cut(s)	582
MaeIII	GTNAC	2 cut(s)	161, 277
MalI	GATC	2 cut(s)	357, 526
MboI	GATC	2 cut(s)	355, 524
MboII	GAAGA	4 cut(s)	149, 359, 432, 912
MfeI	CAATTG	1 cut(s)	286
MlsI	TGGCCA	1 cut(s)	267
MluCI	AATT	3 cut(s)	138, 169, 286
MluNI	TGGCCA	1 cut(s)	267
MlyI	GAGTC	1 cut(s)	315
MmeI	TCCRAC	1 cut(s)	638
Mox20I	TGGCCA	1 cut(s)	267
MscI	TGGCCA	1 cut(s)	267
MseI	TTAA	1 cut(s)	17
MslI	CAYNNNNRTG	1 cut(s)	375
Msp20I	TGGCCA	1 cut(s)	267
MspA1I	CMGCKG	1 cut(s)	963
MspI	CCGG	1 cut(s)	272
MspR9I	CCNGG	3 cut(s)	248, 533, 913
MunI	CAATTG	1 cut(s)	286
MvaI	CCWGG	3 cut(s)	248, 533, 913
MwoI	GCNNNNNNNGC	2 cut(s)	340, 787
NdeI	CATATG	1 cut(s)	934
NdeII	GATC	2 cut(s)	355, 524
NlaIII	CATG	1 cut(s)	235
NlaIV	GGNNCC	1 cut(s)	493
NmuCI	GTSAC	2 cut(s)	161, 277
NspI	RCATGY	1 cut(s)	235
PfeI	GAWTC	2 cut(s)	104, 944
PkrI	GCNGC	2 cut(s)	804, 940
PleI	GAGTC	1 cut(s)	314
PpsI	GAGTC	1 cut(s)	314
Psp6I	CCWGG	3 cut(s)	246, 531, 911
PspGI	CCWGG	3 cut(s)	246, 531, 911
PspN4I	GGNNCC	1 cut(s)	493
PspPI	GGNCC	1 cut(s)	4
PstI	CTGCAG	3 cut(s)	197, 835, 943
RsaI	GTAC	3 cut(s)	230, 257, 493
RsaNI	GTAC	3 cut(s)	229, 256, 492
RseI	CAYNNNNRTG	1 cut(s)	375
SaqAI	TTAA	1 cut(s)	17
SatI	GCNGC	2 cut(s)	803, 939
Sau3AI	GATC	2 cut(s)	355, 524
Sau96I	GGNCC	1 cut(s)	4
SchI	GAGTC	1 cut(s)	315
ScrFI	CCNGG	3 cut(s)	248, 533, 913
SfaNI	GCATC	2 cut(s)	332, 777
SfcI	CTRYAG	5 cut(s)	193, 771, 831, 939, 958
SmiMI	CAYNNNNRTG	1 cut(s)	375
SmlI	CTYRAG	1 cut(s)	677
SmoI	CTYRAG	1 cut(s)	677
Sse9I	AATT	3 cut(s)	138, 169, 286
SsiI	CCGC	2 cut(s)	275, 963
SspI	AATATT	1 cut(s)	673
SspMI	CTAG	2 cut(s)	425, 506
StyD4I	CCNGG	3 cut(s)	246, 531, 911
StyI	CCWWGG	1 cut(s)	782
TaaI	ACNGT	3 cut(s)	461, 775, 927
TaiI	ACGT	1 cut(s)	585
TaqI	TCGA	1 cut(s)	967
TasI	AATT	3 cut(s)	138, 169, 286
TatI	WGTACW	1 cut(s)	228
TfiI	GAWTC	2 cut(s)	104, 944
Tru1I	TTAA	1 cut(s)	17
Tru9I	TTAA	1 cut(s)	17
TseFI	GTSAC	2 cut(s)	161, 277
TseI	GCWGC	2 cut(s)	802, 938
Tsp45I	GTSAC	2 cut(s)	161, 277
TspDTI	ATGAA	2 cut(s)	134, 900
TspGWI	ACGGA	1 cut(s)	432
XapI	RAATTY	1 cut(s)	169
XceI	RCATGY	1 cut(s)	235
XmiI	GTMKAC	1 cut(s)	902
XspI	CTAG	2 cut(s)	425, 506

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.