MD12G1166300.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0018113
Physical Location & Seq
Forward (+)
24698578 .. 24700061
1484 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1166300.v1.1.491

Sequence Viewer

Length: 381 bp
ATGGAGACTCAGAGCTCTGGCGAGCAATCTGTCAGGTCTGTCGCAGCGGGAGGCAACTTCGGATCCGGCATCAGCAAAGCATTTTGCCGGTGCGGCGAAGGATGGAAGTGTGTCATCACTAGGACTGAGGGACCTGATGCCGGCAAGGCCTTCTTCAGCTGTGCTGGAAACTGCACCTGTGTTATCTATGAAGATGGGACAGTGATGAAGGAGGCCATGACGATGAACGAGGAGGTAGAGAAAATTGGTGCAAACGAAGCCTACTGCGAGTGTGGTGAAGGCTGGAAATGTGTCATCTCCAAGGTTGAACCTGAAGATCCTAATGCTGGTAAGAGCTCATTCGAATGTGTTGGTAGTTGCAGCTGTGTAACCGATGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

127

Amino Acids

13.21

Weight (kDa)

4.52

Isoelectric Point (pI)

39.67

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018113)

Species Orthologous Gene IDs
malus_domestica MD12G1166300.v1.1
prunus_persica Prupe.5G034700_v2.0.a1
pyrus_communis pycom12g15840
rosa_chinensis RchiOBHm_Chr7g0232991
rosa_laevigata RLG00000001307 RLG00000017952
rosa_roxburghii Rroxscaffold_2G00131780 Rroxscaffold_3G00227780
rosa_rugosa Rorug01G0313000 Rorug07G0277200
rosa_wichuraiana Rw0G019180

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 47, 93
AclWI GGATC 3 cut(s) 57, 70, 311
AcuI CTGAAG 2 cut(s) 139, 333
AfiI CCNNNNNNNGG 2 cut(s) 140, 326
AgsI TTSAA 1 cut(s) 308
AluBI AGCT 4 cut(s) 15, 159, 336, 363
AluI AGCT 4 cut(s) 15, 159, 336, 363
Alw21I GWGCWC 2 cut(s) 17, 338
AlwI GGATC 3 cut(s) 57, 70, 311
AoxI GGCC 2 cut(s) 147, 213
ApeKI GCWGC 2 cut(s) 44, 360
AspS9I GGNCC 1 cut(s) 131
AsuHPI GGTGA 1 cut(s) 287
AsuII TTCGAA 1 cut(s) 342
AvaII GGWCC 1 cut(s) 131
BamHI GGATCC 1 cut(s) 62
BanII GRGCYC 2 cut(s) 17, 338
Bbv12I GWGCWC 2 cut(s) 17, 338
BbvI GCAGC 2 cut(s) 56, 372
BccI CCATC 3 cut(s) 96, 188, 368
BfaI CTAG 1 cut(s) 120
BglI GCCNNNNNGGC 2 cut(s) 93, 146
BisI GCNGC 3 cut(s) 45, 94, 361
BlsI GCNGC 3 cut(s) 46, 95, 362
Bme18I GGWCC 1 cut(s) 131
BmgT120I GGNCC 1 cut(s) 131
BmiI GGNNCC 2 cut(s) 64, 132
BmsI GCATC 2 cut(s) 78, 127
Bpu14I TTCGAA 1 cut(s) 342
BsaJI CCNNGG 1 cut(s) 300
Bsc4I CCNNNNNNNGG 2 cut(s) 140, 326
Bse118I RCCGGY 2 cut(s) 87, 140
BseDI CCNNGG 1 cut(s) 300
BseGI GGATG 1 cut(s) 107
BseLI CCNNNNNNNGG 2 cut(s) 140, 326
BseMII CTCAG 2 cut(s) 23, 117
BseRI GAGGAG 1 cut(s) 245
BseXI GCAGC 2 cut(s) 56, 372
BsgI GTGCAG 1 cut(s) 157
BshFI GGCC 2 cut(s) 149, 215
BsiHKAI GWGCWC 2 cut(s) 17, 338
BsiSI CCGG 3 cut(s) 66, 88, 141
BslFI GGGAC 2 cut(s) 144, 211
BslI CCNNNNNNNGG 2 cut(s) 140, 326
BsmFI GGGAC 2 cut(s) 144, 211
BsnI GGCC 2 cut(s) 149, 215
Bsp119I TTCGAA 1 cut(s) 342
Bsp1286I GDGCHC 2 cut(s) 17, 338
Bsp143I GATC 2 cut(s) 62, 316
BspACI CCGC 2 cut(s) 47, 93
BspANI GGCC 2 cut(s) 149, 215
BspCNI CTCAG 2 cut(s) 22, 118
BspLI GGNNCC 2 cut(s) 64, 132
BspPI GGATC 3 cut(s) 57, 70, 311
BspT104I TTCGAA 1 cut(s) 342
BsrFI RCCGGY 2 cut(s) 87, 140
BssAI RCCGGY 2 cut(s) 87, 140
BssECI CCNNGG 1 cut(s) 300
BssMI GATC 2 cut(s) 62, 316
BssT1I CCWWGG 1 cut(s) 300
Bst4CI ACNGT 1 cut(s) 202
BstBI TTCGAA 1 cut(s) 342
BstC8I GCNNGC 2 cut(s) 23, 142
BstDEI CTNAG 2 cut(s) 9, 126
BstF5I GGATG 1 cut(s) 107
BstKTI GATC 2 cut(s) 65, 319
BstMBI GATC 2 cut(s) 62, 316
BstMWI GCNNNNNNNGC 3 cut(s) 93, 146, 257
BstV1I GCAGC 2 cut(s) 56, 372
BstX2I RGATCY 2 cut(s) 62, 316
BstYI RGATCY 2 cut(s) 62, 316
BsuRI GGCC 2 cut(s) 149, 215
BtsCI GGATG 1 cut(s) 107
BtsIMutI CAGTG 1 cut(s) 207
Cac8I GCNNGC 2 cut(s) 23, 142
Cfr10I RCCGGY 2 cut(s) 87, 140
Cfr13I GGNCC 1 cut(s) 131
CviAII CATG 1 cut(s) 217
CviJI RGCY 8 cut(s) 15, 149, 159, 215, 260, 282, 336, 363
CviKI_1 RGCY 8 cut(s) 15, 149, 159, 215, 260, 282, 336, 363
DdeI CTNAG 2 cut(s) 9, 126
DpnI GATC 2 cut(s) 64, 318
DpnII GATC 2 cut(s) 62, 316
Ecl136II GAGCTC 2 cut(s) 15, 336
Eco130I CCWWGG 1 cut(s) 300
Eco147I AGGCCT 1 cut(s) 149
Eco24I GRGCYC 2 cut(s) 17, 338
Eco47I GGWCC 1 cut(s) 131
Eco53kI GAGCTC 2 cut(s) 15, 336
Eco57I CTGAAG 2 cut(s) 139, 333
EcoICRI GAGCTC 2 cut(s) 15, 336
EcoO109I RGGNCCY 1 cut(s) 131
EcoT14I CCWWGG 1 cut(s) 300
EcoT38I GRGCYC 2 cut(s) 17, 338
ErhI CCWWGG 1 cut(s) 300
FaeI CATG 1 cut(s) 220
FaiI YATR 2 cut(s) 189, 218
FalI AAGNNNNNCTT 2 cut(s) 137, 169
FaqI GGGAC 2 cut(s) 144, 211
FatI CATG 1 cut(s) 216
FauI CCCGC 1 cut(s) 40
Fnu4HI GCNGC 3 cut(s) 45, 94, 361
FokI GGATG 1 cut(s) 114
FriOI GRGCYC 2 cut(s) 17, 338
Fsp4HI GCNGC 3 cut(s) 45, 94, 361
FspBI CTAG 1 cut(s) 120
GluI GCNGC 3 cut(s) 45, 94, 361
HaeIII GGCC 2 cut(s) 149, 215
HapII CCGG 3 cut(s) 66, 88, 141
Hin1II CATG 1 cut(s) 220
HinfI GANTC 1 cut(s) 7
HpaII CCGG 3 cut(s) 66, 88, 141
HphI GGTGA 1 cut(s) 287
Hpy188I TCNGA 2 cut(s) 12, 62
HpyAV CCTTC 4 cut(s) 92, 160, 202, 272
HpyCH4III ACNGT 1 cut(s) 202
HpyCH4V TGCA 3 cut(s) 174, 251, 360
HpyF10VI GCNNNNNNNGC 3 cut(s) 93, 146, 257
HpyF3I CTNAG 2 cut(s) 9, 126
Hsp92II CATG 1 cut(s) 220
KroI GCCGGC 1 cut(s) 140
KroNI GCCGGC 1 cut(s) 142
Kzo9I GATC 2 cut(s) 62, 316
Lsp1109I GCAGC 2 cut(s) 56, 372
LweI GCATC 2 cut(s) 78, 127
MaeI CTAG 1 cut(s) 120
MaeIII GTNAC 1 cut(s) 367
MalI GATC 2 cut(s) 64, 318
MboI GATC 2 cut(s) 62, 316
MboII GAAGA 3 cut(s) 145, 203, 326
MflI RGATCY 2 cut(s) 62, 316
MhlI GDGCHC 2 cut(s) 17, 338
MluCI AATT 1 cut(s) 243
MnlI CCTC 5 cut(s) 44, 121, 205, 223, 226
MroNI GCCGGC 1 cut(s) 140
MseI TTAA 1 cut(s) 379
MslI CAYNNNNRTG 2 cut(s) 221, 343
MspA1I CMGCKG 3 cut(s) 47, 159, 363
MspI CCGG 3 cut(s) 66, 88, 141
MwoI GCNNNNNNNGC 3 cut(s) 93, 146, 257
NaeI GCCGGC 1 cut(s) 142
NdeII GATC 2 cut(s) 62, 316
NgoMIV GCCGGC 1 cut(s) 140
NlaIII CATG 1 cut(s) 220
NlaIV GGNNCC 2 cut(s) 64, 132
NspV TTCGAA 1 cut(s) 342
PceI AGGCCT 1 cut(s) 149
PdiI GCCGGC 1 cut(s) 142
PkrI GCNGC 3 cut(s) 46, 95, 362
PpuMI RGGWCCY 1 cut(s) 131
Psp124BI GAGCTC 2 cut(s) 17, 338
Psp5II RGGWCCY 1 cut(s) 131
PspN4I GGNNCC 2 cut(s) 64, 132
PspPI GGNCC 1 cut(s) 131
PspPPI RGGWCCY 1 cut(s) 131
PsuI RGATCY 2 cut(s) 62, 316
PvuII CAGCTG 2 cut(s) 159, 363
RseI CAYNNNNRTG 2 cut(s) 221, 343
SacI GAGCTC 2 cut(s) 17, 338
SaqAI TTAA 1 cut(s) 379
SatI GCNGC 3 cut(s) 45, 94, 361
Sau3AI GATC 2 cut(s) 62, 316
Sau96I GGNCC 1 cut(s) 131
SduI GDGCHC 2 cut(s) 17, 338
SfaNI GCATC 2 cut(s) 78, 127
SfuI TTCGAA 1 cut(s) 342
SinI GGWCC 1 cut(s) 131
SmiMI CAYNNNNRTG 2 cut(s) 221, 343
Sse9I AATT 1 cut(s) 243
SseBI AGGCCT 1 cut(s) 149
SsiI CCGC 2 cut(s) 47, 93
SspMI CTAG 1 cut(s) 120
SstI GAGCTC 2 cut(s) 17, 338
StuI AGGCCT 1 cut(s) 149
StyI CCWWGG 1 cut(s) 300
TaaI ACNGT 1 cut(s) 202
TaqI TCGA 1 cut(s) 342
TasI AATT 1 cut(s) 243
TauI GCSGC 1 cut(s) 96
Tru1I TTAA 1 cut(s) 379
Tru9I TTAA 1 cut(s) 379
TscAI CASTG 1 cut(s) 207
TseI GCWGC 2 cut(s) 44, 360
TspDTI ATGAA 3 cut(s) 204, 221, 239
TspRI CASTG 1 cut(s) 207
VpaK11BI GGWCC 1 cut(s) 131
XspI CTAG 1 cut(s) 120
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.