pycom12g15840

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0018113
Physical Location & Seq
Forward (+)
18287259 .. 18288407
1149 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12g15840.2

Sequence Viewer

Length: 375 bp
ATGGAGACTCAGAGCTCTGGCAAGCAATCTGTCAGGTCTGTCGCAGCGGGAGGCAACTCCGGATCCCGCATCAGCAAAGCATTTTGCCGGTGCGGCGAAGGATGGAAGTGTGTCATCACTAGGACTGAGGGACCTGATGCCGGCAAGGCCTTCTTCAGCTGTGCTGGAAAACTACACACCTATGAAGACGGGACAGTGACAAAGGAGGCCACGACGGTGAACGAGGAGGTAGAGAAAATTGGTGCAAACGAAGCCTACTGCGAGTGTGGTGAAGGTTGGAAATGTGTCATCTCCAAGGTTGAACCTGAAGATCCTGATGCTGGCAAGAGCTCCTTCGAATGTGCTGGTAGTTGCAGCTGTGTAACCGATGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

125

Amino Acids

12.98

Weight (kDa)

4.93

Isoelectric Point (pI)

41.02

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0018113)

Species Orthologous Gene IDs
malus_domestica MD12G1166300.v1.1
prunus_persica Prupe.5G034700_v2.0.a1
pyrus_communis pycom12g15840
rosa_chinensis RchiOBHm_Chr7g0232991
rosa_laevigata RLG00000001307 RLG00000017952
rosa_roxburghii Rroxscaffold_2G00131780 Rroxscaffold_3G00227780
rosa_rugosa Rorug01G0313000 Rorug07G0277200
rosa_wichuraiana Rw0G019180

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 59
AciI CCGC 3 cut(s) 47, 67, 93
AclWI GGATC 3 cut(s) 57, 70, 305
AcuI CTGAAG 2 cut(s) 139, 327
AfiI CCNNNNNNNGG 2 cut(s) 140, 320
AgsI TTSAA 1 cut(s) 302
AleI CACNNNNGTG 1 cut(s) 215
AluBI AGCT 4 cut(s) 15, 159, 330, 357
AluI AGCT 4 cut(s) 15, 159, 330, 357
Alw21I GWGCWC 2 cut(s) 17, 332
AlwI GGATC 3 cut(s) 57, 70, 305
Aor13HI TCCGGA 1 cut(s) 59
AoxI GGCC 2 cut(s) 147, 207
ApeKI GCWGC 2 cut(s) 44, 354
AspS9I GGNCC 1 cut(s) 131
AsuHPI GGTGA 2 cut(s) 229, 281
AsuII TTCGAA 1 cut(s) 336
AvaII GGWCC 1 cut(s) 131
BamHI GGATCC 1 cut(s) 62
BanII GRGCYC 2 cut(s) 17, 332
BbsI GAAGAC 1 cut(s) 192
Bbv12I GWGCWC 2 cut(s) 17, 332
BbvI GCAGC 2 cut(s) 56, 366
BccI CCATC 2 cut(s) 96, 362
BfaI CTAG 1 cut(s) 120
BglI GCCNNNNNGGC 2 cut(s) 93, 146
BisI GCNGC 3 cut(s) 45, 94, 355
BlsI GCNGC 3 cut(s) 46, 95, 356
Bme18I GGWCC 1 cut(s) 131
BmgT120I GGNCC 1 cut(s) 131
BmiI GGNNCC 2 cut(s) 64, 132
BmsI GCATC 3 cut(s) 78, 127, 307
BpiI GAAGAC 1 cut(s) 192
Bpu14I TTCGAA 1 cut(s) 336
BsaJI CCNNGG 1 cut(s) 294
BsaWI WCCGGW 1 cut(s) 59
Bsc4I CCNNNNNNNGG 2 cut(s) 140, 320
Bse118I RCCGGY 2 cut(s) 87, 140
BseAI TCCGGA 1 cut(s) 59
BseDI CCNNGG 1 cut(s) 294
BseGI GGATG 1 cut(s) 107
BseLI CCNNNNNNNGG 2 cut(s) 140, 320
BseMII CTCAG 2 cut(s) 23, 117
BseRI GAGGAG 1 cut(s) 239
BseXI GCAGC 2 cut(s) 56, 366
BshFI GGCC 2 cut(s) 149, 209
BsiHKAI GWGCWC 2 cut(s) 17, 332
BsiSI CCGG 3 cut(s) 60, 88, 141
BslFI GGGAC 2 cut(s) 144, 205
BslI CCNNNNNNNGG 2 cut(s) 140, 320
BsmFI GGGAC 2 cut(s) 144, 205
BsnI GGCC 2 cut(s) 149, 209
Bsp119I TTCGAA 1 cut(s) 336
Bsp1286I GDGCHC 2 cut(s) 17, 332
Bsp13I TCCGGA 1 cut(s) 59
Bsp143I GATC 2 cut(s) 62, 310
BspACI CCGC 3 cut(s) 47, 67, 93
BspANI GGCC 2 cut(s) 149, 209
BspCNI CTCAG 2 cut(s) 22, 118
BspEI TCCGGA 1 cut(s) 59
BspLI GGNNCC 2 cut(s) 64, 132
BspPI GGATC 3 cut(s) 57, 70, 305
BspT104I TTCGAA 1 cut(s) 336
BsrFI RCCGGY 2 cut(s) 87, 140
BssAI RCCGGY 2 cut(s) 87, 140
BssECI CCNNGG 1 cut(s) 294
BssMI GATC 2 cut(s) 62, 310
BssT1I CCWWGG 1 cut(s) 294
Bst4CI ACNGT 2 cut(s) 196, 217
BstBI TTCGAA 1 cut(s) 336
BstC8I GCNNGC 3 cut(s) 23, 142, 322
BstDEI CTNAG 2 cut(s) 9, 126
BstF5I GGATG 1 cut(s) 107
BstKTI GATC 2 cut(s) 65, 313
BstMBI GATC 2 cut(s) 62, 310
BstMWI GCNNNNNNNGC 3 cut(s) 93, 146, 251
BstV1I GCAGC 2 cut(s) 56, 366
BstV2I GAAGAC 1 cut(s) 192
BstX2I RGATCY 2 cut(s) 62, 310
BstYI RGATCY 2 cut(s) 62, 310
BsuRI GGCC 2 cut(s) 149, 209
BtsCI GGATG 1 cut(s) 107
BtsIMutI CAGTG 1 cut(s) 201
Cac8I GCNNGC 3 cut(s) 23, 142, 322
Cfr10I RCCGGY 2 cut(s) 87, 140
Cfr13I GGNCC 1 cut(s) 131
CviJI RGCY 7 cut(s) 15, 149, 159, 209, 254, 330, 357
CviKI_1 RGCY 7 cut(s) 15, 149, 159, 209, 254, 330, 357
DdeI CTNAG 2 cut(s) 9, 126
DpnI GATC 2 cut(s) 64, 312
DpnII GATC 2 cut(s) 62, 310
Ecl136II GAGCTC 2 cut(s) 15, 330
Eco130I CCWWGG 1 cut(s) 294
Eco147I AGGCCT 1 cut(s) 149
Eco24I GRGCYC 2 cut(s) 17, 332
Eco47I GGWCC 1 cut(s) 131
Eco53kI GAGCTC 2 cut(s) 15, 330
Eco57I CTGAAG 2 cut(s) 139, 327
EcoICRI GAGCTC 2 cut(s) 15, 330
EcoO109I RGGNCCY 1 cut(s) 131
EcoT14I CCWWGG 1 cut(s) 294
EcoT38I GRGCYC 2 cut(s) 17, 332
ErhI CCWWGG 1 cut(s) 294
FaiI YATR 1 cut(s) 183
FalI AAGNNNNNCTT 4 cut(s) 137, 169, 317, 349
FaqI GGGAC 2 cut(s) 144, 205
FauI CCCGC 2 cut(s) 40, 74
Fnu4HI GCNGC 3 cut(s) 45, 94, 355
FokI GGATG 1 cut(s) 114
FriOI GRGCYC 2 cut(s) 17, 332
Fsp4HI GCNGC 3 cut(s) 45, 94, 355
FspBI CTAG 1 cut(s) 120
GluI GCNGC 3 cut(s) 45, 94, 355
HaeIII GGCC 2 cut(s) 149, 209
HapII CCGG 3 cut(s) 60, 88, 141
HinfI GANTC 1 cut(s) 7
HpaII CCGG 3 cut(s) 60, 88, 141
HphI GGTGA 2 cut(s) 229, 281
Hpy166II GTNNAC 1 cut(s) 220
Hpy188I TCNGA 1 cut(s) 12
Hpy188III TCNNGA 2 cut(s) 60, 314
Hpy8I GTNNAC 1 cut(s) 220
Hpy99I CGWCG 1 cut(s) 217
HpyAV CCTTC 4 cut(s) 92, 160, 266, 343
HpyCH4III ACNGT 2 cut(s) 196, 217
HpyCH4V TGCA 2 cut(s) 245, 354
HpyF10VI GCNNNNNNNGC 3 cut(s) 93, 146, 251
HpyF3I CTNAG 2 cut(s) 9, 126
Kpn2I TCCGGA 1 cut(s) 59
KroI GCCGGC 1 cut(s) 140
KroNI GCCGGC 1 cut(s) 142
Kzo9I GATC 2 cut(s) 62, 310
LmnI GCTCC 1 cut(s) 335
Lsp1109I GCAGC 2 cut(s) 56, 366
LweI GCATC 3 cut(s) 78, 127, 307
MaeI CTAG 1 cut(s) 120
MaeIII GTNAC 2 cut(s) 196, 361
MalI GATC 2 cut(s) 64, 312
MboI GATC 2 cut(s) 62, 310
MboII GAAGA 3 cut(s) 145, 197, 320
MflI RGATCY 2 cut(s) 62, 310
MhlI GDGCHC 2 cut(s) 17, 332
MluCI AATT 1 cut(s) 237
MmeI TCCRAC 1 cut(s) 257
MnlI CCTC 5 cut(s) 44, 121, 199, 217, 220
MroI TCCGGA 1 cut(s) 59
MroNI GCCGGC 1 cut(s) 140
MseI TTAA 1 cut(s) 373
MslI CAYNNNNRTG 2 cut(s) 180, 215
MspA1I CMGCKG 3 cut(s) 47, 159, 357
MspI CCGG 3 cut(s) 60, 88, 141
MwoI GCNNNNNNNGC 3 cut(s) 93, 146, 251
NaeI GCCGGC 1 cut(s) 142
NdeII GATC 2 cut(s) 62, 310
NgoMIV GCCGGC 1 cut(s) 140
NlaIV GGNNCC 2 cut(s) 64, 132
NmuCI GTSAC 1 cut(s) 196
NspV TTCGAA 1 cut(s) 336
OliI CACNNNNGTG 1 cut(s) 215
PceI AGGCCT 1 cut(s) 149
PdiI GCCGGC 1 cut(s) 142
PkrI GCNGC 3 cut(s) 46, 95, 356
PpuMI RGGWCCY 1 cut(s) 131
Psp124BI GAGCTC 2 cut(s) 17, 332
Psp5II RGGWCCY 1 cut(s) 131
PspN4I GGNNCC 2 cut(s) 64, 132
PspPI GGNCC 1 cut(s) 131
PspPPI RGGWCCY 1 cut(s) 131
PsuI RGATCY 2 cut(s) 62, 310
PvuII CAGCTG 2 cut(s) 159, 357
RseI CAYNNNNRTG 2 cut(s) 180, 215
SacI GAGCTC 2 cut(s) 17, 332
SaqAI TTAA 1 cut(s) 373
SatI GCNGC 3 cut(s) 45, 94, 355
Sau3AI GATC 2 cut(s) 62, 310
Sau96I GGNCC 1 cut(s) 131
SduI GDGCHC 2 cut(s) 17, 332
SfaNI GCATC 3 cut(s) 78, 127, 307
SfuI TTCGAA 1 cut(s) 336
SinI GGWCC 1 cut(s) 131
SmiMI CAYNNNNRTG 2 cut(s) 180, 215
Sse9I AATT 1 cut(s) 237
SseBI AGGCCT 1 cut(s) 149
SsiI CCGC 3 cut(s) 47, 67, 93
SspMI CTAG 1 cut(s) 120
SstI GAGCTC 2 cut(s) 17, 332
StuI AGGCCT 1 cut(s) 149
StyI CCWWGG 1 cut(s) 294
TaaI ACNGT 2 cut(s) 196, 217
TaqI TCGA 1 cut(s) 336
TasI AATT 1 cut(s) 237
TauI GCSGC 1 cut(s) 96
Tru1I TTAA 1 cut(s) 373
Tru9I TTAA 1 cut(s) 373
TscAI CASTG 1 cut(s) 201
TseFI GTSAC 1 cut(s) 196
TseI GCWGC 2 cut(s) 44, 354
Tsp45I GTSAC 1 cut(s) 196
TspDTI ATGAA 1 cut(s) 198
TspRI CASTG 1 cut(s) 201
VpaK11BI GGWCC 1 cut(s) 131
XspI CTAG 1 cut(s) 120
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.