Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0005401

Physical Location & Seq

Genomic Coordinates Reverse (-)

26604335 .. 26609262

Gene Structure 4928 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1185300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2643 bp

ATGTGTATACTTTGTGTGATTCAGAAGTGGTCTCGCCGGGTTGCTACAATGCTTCCTTGGTTAGTTATTCCATTAATAGGACTGTGGGGTCTCTCTCAGCTTCTACCACCAGCTTTCCGATTTGAGATAACTTCGCCTAGACTCGCTTGTGTTGTTGTGCTCTTGGTTACTCTCTTCTGGTATGAGGTTTTGATGCCTCAGCTTTCAGCTTGGCGCTTGCGGAGGAATGCAATGCTTAGAGAGAGGAAAAGGTTTGAGGCAATAGAGTTGCAAAAGCTGAGGAAAACAGCAACAAGGCGGTGTCGAAACTGCTCGACACCATATAGGGATCAGAATCCTGGTGGTGGTCGGTTTATGTGTTCGTATTGTGGGCATTTATCAAAGAGACCGGTTTTGGACTTACCTGAGGTACCCGGGATGGGACTTTCGAAGTCTGGGATCATCAAAGATTTAGTTGGAAAGGGTGGAAAGATATTGAATGGAAAGGCGTGGTCTGAAAATGGGTGGATGCAAGGTCAAGATTGGTCGGAGAATGGAAATTGGGTCAATGGTTCTGTTGGAGGAAAGTCTAGTTATTGGAGAAAGGACGGGAGCGGTGTTTTTGGAGCGGATGAGAGTTGCTTGGCAGAAAAGTCGTATTCAGGTGTTGTTAATTTTGCTTGCAAGCTACTAACCTTTTTTTTCTTGAGTGTTAGATGGCTTTGGAGAAAGCTTTTTAGTAGTAGTACATCAGATGATGCATCTGACGGAGATCGTAAAGGATTGGCCAAGAGGGGCGAGAATGGGGCCAACTTGAATGAAAGTAGAGGAGAAAAGGCCCGCAGAAAAGCTGAAGAGAAGAGACAGGCTAAGATAGAGAAGGAGCTTTGGGAGGAGGAAGAAAGAAAGCAGAGGGAGGAAGTTGCAAGGTTGGTGGAGGAACGCAGGAGACTTAGGGATGAGAAAAAGGAAGCTGAACGTGGGAAATCATCACCACCTGTGATTGAAAAAGATACTAAGAAGGAAGCAGAAAAGAAACGACAGGAAAGAAGGAAAGAGAAAGACAAGGGTTCTAGCAAGAGCAACTCTGATGCAGAAGAGCTGGAAAAGAGAGCAGGTAAGGAAAATGAACGAAAGCGGGACCTTGACAAGAAGAGTGACATTGACCGCAGGGAACATCTGAAATCAGGGGCAGATTTTCTTAAGGGACAAAGCACGGAGATGGGGAATAATATAAAAAATGCATCTGCAAACAATTTTATTCAGGGAAATGCTGGATCTAGGTACCTGGACCGCATGAGGGGTACGATTTTTAATTCTTCAAAGGCATTTGGTGGAGGTAGTTTCTTTGGAAAGGATGCTAATACTACAATGACAAAAGAAACCAAATCTAGCAGTTCTGTAGATCATGTCCATAGTTATTATGCCCAGAAGAGAGACTTATGTCCACCTGAGCGCGTAGCTGCAAGACCTTTTATTAATGGAGATGATAAGAGTGTCCACCGCCCTGTTCACTCAGAACCACAGTCAGGGACTGCACCTAAAAAATCATGGCAGCAGTTGTTTACTCGTTCATCGTCAGTGCCTTCATCATCAAGTGTAAATGTAATAAGTAGACCAAAAGCAAAGTCTCAGACAAAAGTTCAAAGTTCTCAGTTATCTGGCCAGTCGTCATCAATCCAATCATTTGATAATCCAATCAACTTTGGGCTGCCATCACCATTCGCTTTATCTACTACCTATCCAAAAGGATCTACTAGCAGCAGTTTAGGTTTTTCACCCGCAATTGATCCCATATTTCCTCACATTGGAGAAGGACATCATGAACTTATACCCGAGGAGCCAGAGCTTTTTGAAGACCCAAGTTACGTCCCTGATCCGGTATCCTTGCTTGGGCCAGTTTCTGAGTCACTTGATAATTTTCAATTAAACATGGGATTGGAGAGGCCTCGCACATTGAAGAATGGACCTGCTTCATCTGAAGTGAACAAGCCATCTCCAATCGAGTCACCAATGTCGCGAGAAAAGCTTAATAATTCTAGTCGCTTCCCTAGTACCCCAAAGGCCCATGATATGCATGCTTACCCCTTGGATGATGCAAGTGCAAATGATAAGGGAACGTGGCAAATGTGGAACAGTTGCCCTCTTGGTCAGGAAGGTCTAGGTTTTGCAGGTGGCTCTCCAAGCTGGTTTCTACCTCCAGAACTGAACAGATCAAACAAGGATGATCTTTTGCACCCATCATCAGTATCACTGTTTACTACAGAAGATCAGGTTGTTTCTGGCTCTCAATCTCCTAAGAATCAGAGAATTTTTCTTGGAAATGGCCAGAATGGCGGAACCTTTAGCCCTGTTACTGGTTCCGGTGATCATGATCCTTGGTTGCAGAAAGCCTTTTTTCCTCCATTGTCAACAGCTGAAAACCATTTTCATCTGAGACCTCCAGACGAAACTTCGAAGAATGAACTGATTTTTGGGAGTCCCATCAGAGCTACTGGTAACCATCCATTTGAGCTGCCTCAAGCGAATGGTTGGTCCGAAAAGGAATGGGATGAATGCGCTGTGACGGGAACAGGGGACGGTGTTGGAAAGCCATATGTGTTGAGGCCTCCTGTCAGGGGTATGTATCCGACCCCGAATCCAAATGTACAGCCGCTTTGGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

No domains found.

Protein Analysis

881

Amino Acids

98.13

Weight (kDa)

9.26

Isoelectric Point (pI)

58.65

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005401)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G51640 AT3G51640 AT3G51640 AT3G51650 AT3G51650 AT3G51650 AT3G51650
fragaria_vesca	FvH4_6g10170 FvH4_6g10170 FvH4_6g10170
malus_domestica	MD04G1172000.v1.1 MD12G1185300.v1.1
prunus_persica	Prupe.6G290600_v2.0.a1 Prupe.6G290600_v2.0.a1
pyrus_communis	pycom04g15200 pycom12g17380
rosa_chinensis	RchiOBHm_Chr3g0460911
rosa_laevigata	RLG00000024966
rosa_multiflora	Rmu_sc0008258.1_g000012 Rmu_sc0021377.1_g000004
rosa_roxburghii	Rroxscaffold_6G00419040
rosa_rugosa	Rorug03G0047000 Rorug03G0047000 Rorug03G0047000
rosa_samantha	Rh3AG104400 Rh3BG108100 Rh3CG109800 Rh3DG109900
rosa_wichuraiana	Rw3G008890

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	2141
AasI	GACNNNNNNGTC	1 cut(s)	87
Acc36I	ACCTGC	3 cut(s)	1085, 1957, 2141
Acc65I	GGTACC	2 cut(s)	409, 1263
AccB1I	GGYRCC	2 cut(s)	409, 1263
AccBSI	CCGCTC	2 cut(s)	594, 608
AccI	GTMKAC	2 cut(s)	7, 1594
AccII	CGCG	2 cut(s)	1437, 1999
AclWI	GGATC	7 cut(s)	336, 446, 1264, 1738, 1763, 1850, 2348
AcoI	YGGCCR	3 cut(s)	765, 1642, 2305
AcsI	RAATTY	1 cut(s)	2289
AcuI	CTGAAG	2 cut(s)	852, 1980
AfaI	GTAC	6 cut(s)	411, 727, 1265, 1285, 2035, 2628
AflII	CTTAAG	1 cut(s)	1181
AgeI	ACCGGT	1 cut(s)	388
AgsI	TTSAA	8 cut(s)	478, 796, 986, 1302, 1625, 1835, 1904, 1939
AjnI	CCWGG	2 cut(s)	337, 1266
AjuI	GAANNNNNNNTTGG	2 cut(s)	2436, 2468
Alw21I	GWGCWC	1 cut(s)	162
Alw26I	GTCTC	8 cut(s)	36, 95, 379, 835, 922, 1410, 1614, 2410
AlwI	GGATC	7 cut(s)	336, 446, 1264, 1738, 1763, 1850, 2348
AlwNI	CAGNNNCTG	2 cut(s)	1514, 1883
Ama87I	CYCGRG	2 cut(s)	413, 1814
AoxI	GGCC	9 cut(s)	765, 786, 816, 1642, 1874, 1925, 2043, 2305, 2585
ApeKI	GCWGC	5 cut(s)	1442, 1534, 1690, 1740, 2494
ApoI	RAATTY	1 cut(s)	2289
ArsI	GACNNNNNNTTYG	4 cut(s)	1592, 1624, 2498, 2530
AseI	ATTAAT	2 cut(s)	74, 1458
AsiGI	ACCGGT	1 cut(s)	388
Asp718I	GGTACC	2 cut(s)	409, 1263
AspLEI	GCGC	3 cut(s)	216, 1437, 2540
AspS9I	GGNCC	8 cut(s)	786, 817, 1120, 1270, 1874, 1946, 2044, 2514
AsuC2I	CCSGG	3 cut(s)	38, 414, 415
AsuHPI	GGTGA	5 cut(s)	963, 1689, 1749, 1980, 2357
AsuII	TTCGAA	2 cut(s)	428, 2435
AvaI	CYCGRG	2 cut(s)	413, 1814
AvaII	GGWCC	4 cut(s)	1120, 1270, 1946, 2514
AxyI	CCTNAGG	1 cut(s)	405
BalI	TGGCCA	3 cut(s)	767, 1644, 2307
BanI	GGYRCC	2 cut(s)	409, 1263
BbsI	GAAGAC	1 cut(s)	1842
Bbv12I	GWGCWC	1 cut(s)	162
BbvCI	CCTCAGC	2 cut(s)	198, 278
BbvI	GCAGC	5 cut(s)	1429, 1546, 1677, 1752, 2481
BccI	CCATC	8 cut(s)	412, 690, 1195, 1702, 1981, 2227, 2471, 2490
BcgI	CGANNNNNNTGC	2 cut(s)	615, 649
BciT130I	CCWGG	2 cut(s)	339, 1268
BciVI	GTATCC	2 cut(s)	1873, 2616
BclI	TGATCA	1 cut(s)	2347
BcnI	CCSGG	3 cut(s)	38, 414, 415
BcoDI	GTCTC	8 cut(s)	36, 95, 379, 835, 922, 1410, 1614, 2410
BfaI	CTAG	9 cut(s)	138, 570, 1053, 1260, 1371, 1737, 2019, 2031, 2141
BfmI	CTRYAG	2 cut(s)	1380, 2241
BfoI	RGCGCY	1 cut(s)	217
BfrI	CTTAAG	1 cut(s)	1181
BfuAI	ACCTGC	3 cut(s)	1085, 1957, 2141
BfuI	GTATCC	2 cut(s)	1873, 2616
BglI	GCCNNNNNGGC	1 cut(s)	2313
BisI	GCNGC	6 cut(s)	1443, 1535, 1691, 1741, 2495, 2633
BlsI	GCNGC	6 cut(s)	1444, 1536, 1692, 1742, 2496, 2634
Bme1390I	CCNGG	5 cut(s)	38, 339, 414, 415, 1268
Bme18I	GGWCC	4 cut(s)	1120, 1270, 1946, 2514
BmeT110I	CYCGRG	2 cut(s)	413, 1814
BmgT120I	GGNCC	8 cut(s)	786, 817, 1120, 1270, 1874, 1946, 2044, 2514
BmiI	GGNNCC	7 cut(s)	411, 787, 1121, 1265, 1821, 2320, 2341
BmrFI	CCNGG	5 cut(s)	38, 339, 414, 415, 1268
BmsI	GCATC	8 cut(s)	183, 498, 727, 749, 1060, 1232, 1327, 2065
BoxI	GACNNNNGTC	1 cut(s)	1422
BpiI	GAAGAC	1 cut(s)	1842
BpmI	CTGGAG	2 cut(s)	2163, 2406
Bpu10I	CCTNAGC	3 cut(s)	198, 278, 1431
Bpu14I	TTCGAA	2 cut(s)	428, 2435
BpuEI	CTTGAG	2 cut(s)	706, 2484
BpuMI	CCSGG	3 cut(s)	38, 414, 415
BsaBI	GATNNNNATC	2 cut(s)	333, 2352
BsaI	GGTCTC	4 cut(s)	36, 95, 379, 2410
BsaJI	CCNNGG	5 cut(s)	56, 413, 1815, 2067, 2357
BsaWI	WCCGGW	3 cut(s)	388, 1858, 2342
Bse118I	RCCGGY	1 cut(s)	388
Bse1I	ACTGG	4 cut(s)	1645, 1877, 2341, 2479
Bse21I	CCTNAGG	1 cut(s)	405
Bse3DI	GCAATG	1 cut(s)	237
Bse8I	GATNNNNATC	2 cut(s)	333, 2352
BseBI	CCWGG	2 cut(s)	339, 1268
BseDI	CCNNGG	5 cut(s)	56, 413, 1815, 2067, 2357
BseGI	GGATG	9 cut(s)	423, 513, 616, 943, 1342, 2077, 2209, 2482, 2536
BseJI	GATNNNNATC	2 cut(s)	333, 2352
BseMI	GCAATG	1 cut(s)	237
BseNI	ACTGG	4 cut(s)	1645, 1877, 2341, 2479
BseRI	GAGGAG	3 cut(s)	822, 887, 1832
BseXI	GCAGC	5 cut(s)	1429, 1546, 1677, 1752, 2481
BsgI	GTGCAG	1 cut(s)	1500
Bsh1236I	CGCG	2 cut(s)	1437, 1999
BshFI	GGCC	9 cut(s)	767, 788, 818, 1644, 1876, 1927, 2045, 2307, 2587
BshNI	GGYRCC	2 cut(s)	409, 1263
BshTI	ACCGGT	1 cut(s)	388
BsiHKAI	GWGCWC	1 cut(s)	162
BsiHKCI	CYCGRG	2 cut(s)	413, 1814
BsiSI	CCGG	5 cut(s)	37, 389, 414, 1859, 2343
BslFI	GGGAC	7 cut(s)	435, 1133, 1200, 1525, 1835, 2445, 2570
BsmAI	GTCTC	8 cut(s)	36, 95, 379, 835, 922, 1410, 1614, 2410
BsmFI	GGGAC	7 cut(s)	435, 1133, 1200, 1525, 1835, 2445, 2570
BsmI	GAATGC	2 cut(s)	232, 2540
BsnI	GGCC	9 cut(s)	767, 788, 818, 1644, 1876, 1927, 2045, 2307, 2587
Bso31I	GGTCTC	4 cut(s)	36, 95, 379, 2410
BsoBI	CYCGRG	2 cut(s)	413, 1814
Bsp119I	TTCGAA	2 cut(s)	428, 2435
Bsp1286I	GDGCHC	1 cut(s)	162
Bsp1407I	TGTACA	1 cut(s)	2626
Bsp68I	TCGCGA	1 cut(s)	1999
BspANI	GGCC	9 cut(s)	767, 788, 818, 1644, 1876, 1927, 2045, 2307, 2587
BspFNI	CGCG	2 cut(s)	1437, 1999
BspHI	TCATGA	2 cut(s)	1801, 2350
BspLI	GGNNCC	7 cut(s)	411, 787, 1121, 1265, 1821, 2320, 2341
BspMI	ACCTGC	3 cut(s)	1085, 1957, 2141
BspPI	GGATC	7 cut(s)	336, 446, 1264, 1738, 1763, 1850, 2348
BspQI	GCTCTTC	1 cut(s)	1071
BspT104I	TTCGAA	2 cut(s)	428, 2435
BspT107I	GGYRCC	2 cut(s)	409, 1263
BspTI	CTTAAG	1 cut(s)	1181
BspTNI	GGTCTC	4 cut(s)	36, 95, 379, 2410
BsrBI	CCGCTC	2 cut(s)	594, 608
BsrDI	GCAATG	1 cut(s)	237
BsrFI	RCCGGY	1 cut(s)	388
BsrGI	TGTACA	1 cut(s)	2626
BsrI	ACTGG	4 cut(s)	1645, 1877, 2341, 2479
BssAI	RCCGGY	1 cut(s)	388
BssECI	CCNNGG	5 cut(s)	56, 413, 1815, 2067, 2357
BssNAI	GTATAC	1 cut(s)	8
BssT1I	CCWWGG	3 cut(s)	56, 2067, 2357
Bst1107I	GTATAC	1 cut(s)	8
Bst2UI	CCWGG	2 cut(s)	339, 1268
Bst4CI	ACNGT	5 cut(s)	84, 1506, 2117, 2235, 2561
Bst6I	CTCTTC	6 cut(s)	179, 828, 833, 1071, 1127, 1406
BstAFI	CTTAAG	1 cut(s)	1181
BstAUI	TGTACA	1 cut(s)	2626
BstBI	TTCGAA	2 cut(s)	428, 2435
BstC8I	GCNNGC	5 cut(s)	218, 661, 665, 820, 2058
BstEII	GGTNACC	1 cut(s)	2477
BstF5I	GGATG	9 cut(s)	423, 513, 616, 943, 1342, 2077, 2209, 2482, 2536
BstFNI	CGCG	2 cut(s)	1437, 1999
BstH2I	RGCGCY	1 cut(s)	217
BstHHI	GCGC	3 cut(s)	216, 1437, 2540
BstMAI	GTCTC	8 cut(s)	36, 95, 379, 835, 922, 1410, 1614, 2410
BstMWI	GCNNNNNNNGC	3 cut(s)	2005, 2163, 2313
BstNI	CCWGG	2 cut(s)	339, 1268
BstNSI	RCATGY	1 cut(s)	2060
BstPAI	GACNNNNGTC	1 cut(s)	1422
BstPI	GGTNACC	1 cut(s)	2477
BstSCI	CCNGG	5 cut(s)	36, 337, 412, 413, 1266
BstSFI	CTRYAG	2 cut(s)	1380, 2241
BstUI	CGCG	2 cut(s)	1437, 1999
BstV1I	GCAGC	5 cut(s)	1429, 1546, 1677, 1752, 2481
BstV2I	GAAGAC	1 cut(s)	1842
BstX2I	RGATCY	2 cut(s)	1256, 1730
BstYI	RGATCY	2 cut(s)	1256, 1730
BstZ17I	GTATAC	1 cut(s)	8
Bsu36I	CCTNAGG	1 cut(s)	405
BsuI	GTATCC	2 cut(s)	1873, 2616
BsuRI	GGCC	9 cut(s)	767, 788, 818, 1644, 1876, 1927, 2045, 2307, 2587
BtsCI	GGATG	9 cut(s)	423, 513, 616, 943, 1342, 2077, 2209, 2482, 2536
BtsIMutI	CAGTG	2 cut(s)	1566, 2231
BtuMI	TCGCGA	1 cut(s)	1999
BveI	ACCTGC	3 cut(s)	1085, 1957, 2141
Cac8I	GCNNGC	5 cut(s)	218, 661, 665, 820, 2058
CaiI	CAGNNNCTG	2 cut(s)	1514, 1883
CciI	TCATGA	2 cut(s)	1801, 2350
CfoI	GCGC	3 cut(s)	216, 1437, 2540
Cfr10I	RCCGGY	1 cut(s)	388
Cfr13I	GGNCC	8 cut(s)	786, 817, 1120, 1270, 1874, 1946, 2044, 2514
Cfr9I	CCCGGG	1 cut(s)	413
Csp6I	GTAC	6 cut(s)	410, 726, 1264, 1284, 2034, 2627
CspAI	ACCGGT	1 cut(s)	388
CspCI	CAANNNNNGTGG	2 cut(s)	894, 929
CviAII	CATG	8 cut(s)	1276, 1388, 1530, 1802, 1912, 2048, 2057, 2351
CviQI	GTAC	6 cut(s)	410, 726, 1264, 1284, 2034, 2627
DrdI	GACNNNNNNGTC	1 cut(s)	87
DseDI	GACNNNNNNGTC	1 cut(s)	87
EaeI	YGGCCR	3 cut(s)	765, 1642, 2305
Eam1104I	CTCTTC	6 cut(s)	179, 828, 833, 1071, 1127, 1406
EarI	CTCTTC	6 cut(s)	179, 828, 833, 1071, 1127, 1406
EciI	GGCGGA	1 cut(s)	2331
Eco130I	CCWWGG	3 cut(s)	56, 2067, 2357
Eco147I	AGGCCT	2 cut(s)	1927, 2587
Eco31I	GGTCTC	4 cut(s)	36, 95, 379, 2410
Eco47I	GGWCC	4 cut(s)	1120, 1270, 1946, 2514
Eco57I	CTGAAG	2 cut(s)	852, 1980
Eco81I	CCTNAGG	1 cut(s)	405
Eco88I	CYCGRG	2 cut(s)	413, 1814
Eco91I	GGTNACC	1 cut(s)	2477
EcoO109I	RGGNCCY	1 cut(s)	1120
EcoO65I	GGTNACC	1 cut(s)	2477
EcoRII	CCWGG	2 cut(s)	337, 1266
EcoT14I	CCWWGG	3 cut(s)	56, 2067, 2357
EcoT22I	ATGCAT	3 cut(s)	742, 1225, 2058
ErhI	CCWWGG	3 cut(s)	56, 2067, 2357
FaeI	CATG	8 cut(s)	1279, 1391, 1533, 1805, 1915, 2051, 2060, 2354
FalI	AAGNNNNNCTT	2 cut(s)	1403, 1435
FaqI	GGGAC	7 cut(s)	435, 1133, 1200, 1525, 1835, 2445, 2570
FatI	CATG	8 cut(s)	1275, 1387, 1529, 1801, 1911, 2047, 2056, 2350
FauI	CCCGC	3 cut(s)	827, 1110, 1768
FauNDI	CATATG	1 cut(s)	2575
FbaI	TGATCA	1 cut(s)	2347
FblI	GTMKAC	2 cut(s)	7, 1594
Fnu4HI	GCNGC	6 cut(s)	1443, 1535, 1691, 1741, 2495, 2633
FokI	GGATG	9 cut(s)	430, 520, 623, 950, 1349, 2084, 2216, 2469, 2543
Fsp4HI	GCNGC	6 cut(s)	1443, 1535, 1691, 1741, 2495, 2633
FspBI	CTAG	9 cut(s)	138, 570, 1053, 1260, 1371, 1737, 2019, 2031, 2141
GlaI	GCGC	3 cut(s)	215, 1436, 2539
GluI	GCNGC	6 cut(s)	1443, 1535, 1691, 1741, 2495, 2633
GsuI	CTGGAG	2 cut(s)	2163, 2406
HaeII	RGCGCY	1 cut(s)	217
HaeIII	GGCC	9 cut(s)	767, 788, 818, 1644, 1876, 1927, 2045, 2307, 2587
HapII	CCGG	5 cut(s)	37, 389, 414, 1859, 2343
HhaI	GCGC	3 cut(s)	216, 1437, 2540
Hin1II	CATG	8 cut(s)	1279, 1391, 1533, 1805, 1915, 2051, 2060, 2354
Hin6I	GCGC	3 cut(s)	214, 1435, 2538
HinP1I	GCGC	3 cut(s)	214, 1435, 2538
HincII	GTYRAC	1 cut(s)	2391
HindII	GTYRAC	1 cut(s)	2391
HindIII	AAGCTT	2 cut(s)	710, 2006
HinfI	GANTC	8 cut(s)	19, 141, 334, 1886, 1985, 2281, 2458, 2617
HpaII	CCGG	5 cut(s)	37, 389, 414, 1859, 2343
HphI	GGTGA	5 cut(s)	963, 1689, 1749, 1980, 2357
Hpy166II	GTNNAC	9 cut(s)	8, 1427, 1480, 1492, 1545, 1595, 1966, 2238, 2391
Hpy188III	TCNNGA	8 cut(s)	518, 685, 1802, 1998, 2132, 2180, 2351, 2423
Hpy8I	GTNNAC	9 cut(s)	8, 1427, 1480, 1492, 1545, 1595, 1966, 2238, 2391
HpyAV	CCTTC	6 cut(s)	853, 994, 1023, 1575, 1787, 2129
HpyCH4III	ACNGT	5 cut(s)	84, 1506, 2117, 2235, 2561
HpyCH4IV	ACGT	3 cut(s)	958, 1848, 2099
HpyF10VI	GCNNNNNNNGC	3 cut(s)	2005, 2163, 2313
HpySE526I	ACGT	3 cut(s)	958, 1848, 2099
Hsp92II	CATG	8 cut(s)	1279, 1391, 1533, 1805, 1915, 2051, 2060, 2354
HspAI	GCGC	3 cut(s)	214, 1435, 2538
KpnI	GGTACC	2 cut(s)	413, 1267
Ksp22I	TGATCA	1 cut(s)	2347
LguI	GCTCTTC	1 cut(s)	1071
LmnI	GCTCC	4 cut(s)	591, 605, 862, 1819
Lsp1109I	GCAGC	5 cut(s)	1429, 1546, 1677, 1752, 2481
LweI	GCATC	8 cut(s)	183, 498, 727, 749, 1060, 1232, 1327, 2065
MaeI	CTAG	9 cut(s)	138, 570, 1053, 1260, 1371, 1737, 2019, 2031, 2141
MaeII	ACGT	3 cut(s)	958, 1848, 2099
MaeIII	GTNAC	8 cut(s)	166, 1136, 1844, 1887, 1986, 2332, 2477, 2542
MbiI	CCGCTC	2 cut(s)	594, 608
MfeI	CAATTG	1 cut(s)	1764
MflI	RGATCY	2 cut(s)	1256, 1730
MhlI	GDGCHC	1 cut(s)	162
MlsI	TGGCCA	3 cut(s)	767, 1644, 2307
MluCI	AATT	9 cut(s)	538, 652, 1234, 1294, 1764, 1897, 1904, 2014, 2289
MluNI	TGGCCA	3 cut(s)	767, 1644, 2307
MlyI	GAGTC	4 cut(s)	135, 1895, 1994, 2467
MmeI	TCCRAC	5 cut(s)	436, 507, 538, 2545, 2633
Mox20I	TGGCCA	3 cut(s)	767, 1644, 2307
Mph1103I	ATGCAT	3 cut(s)	742, 1225, 2058
MscI	TGGCCA	3 cut(s)	767, 1644, 2307
MseI	TTAA	7 cut(s)	74, 651, 1182, 1293, 1458, 1907, 2010
MslI	CAYNNNNRTG	1 cut(s)	1199
Msp20I	TGGCCA	3 cut(s)	767, 1644, 2307
MspA1I	CMGCKG	1 cut(s)	2396
MspCI	CTTAAG	1 cut(s)	1181
MspI	CCGG	5 cut(s)	37, 389, 414, 1859, 2343
MspR9I	CCNGG	5 cut(s)	38, 339, 414, 415, 1268
MunI	CAATTG	1 cut(s)	1764
Mva1269I	GAATGC	2 cut(s)	232, 2540
MvaI	CCWGG	2 cut(s)	339, 1268
MvnI	CGCG	2 cut(s)	1437, 1999
MwoI	GCNNNNNNNGC	3 cut(s)	2005, 2163, 2313
NciI	CCSGG	3 cut(s)	38, 414, 415
NdeI	CATATG	1 cut(s)	2575
NlaIII	CATG	8 cut(s)	1279, 1391, 1533, 1805, 1915, 2051, 2060, 2354
NlaIV	GGNNCC	7 cut(s)	411, 787, 1121, 1265, 1821, 2320, 2341
NmuCI	GTSAC	4 cut(s)	1136, 1887, 1986, 2542
NruI	TCGCGA	1 cut(s)	1999
NsiI	ATGCAT	3 cut(s)	742, 1225, 2058
NspI	RCATGY	1 cut(s)	2060
NspV	TTCGAA	2 cut(s)	428, 2435
PaeI	GCATGC	1 cut(s)	2060
PagI	TCATGA	2 cut(s)	1801, 2350
PaqCI	CACCTGC	1 cut(s)	2141
PceI	AGGCCT	2 cut(s)	1927, 2587
PciSI	GCTCTTC	1 cut(s)	1071
PctI	GAATGC	2 cut(s)	232, 2540
PfeI	GAWTC	4 cut(s)	19, 334, 2281, 2617
PinAI	ACCGGT	1 cut(s)	388
PkrI	GCNGC	6 cut(s)	1444, 1536, 1692, 1742, 2496, 2634
PleI	GAGTC	4 cut(s)	135, 1894, 1993, 2466
PpsI	GAGTC	4 cut(s)	135, 1894, 1993, 2466
PpuMI	RGGWCCY	1 cut(s)	1120
PshAI	GACNNNNGTC	1 cut(s)	1422
PshBI	ATTAAT	2 cut(s)	74, 1458
Psp5II	RGGWCCY	1 cut(s)	1120
Psp6I	CCWGG	2 cut(s)	337, 1266
PspEI	GGTNACC	1 cut(s)	2477
PspGI	CCWGG	2 cut(s)	337, 1266
PspN4I	GGNNCC	7 cut(s)	411, 787, 1121, 1265, 1821, 2320, 2341
PspPI	GGNCC	8 cut(s)	786, 817, 1120, 1270, 1874, 1946, 2044, 2514
PspPPI	RGGWCCY	1 cut(s)	1120
PstNI	CAGNNNCTG	2 cut(s)	1514, 1883
PsuI	RGATCY	2 cut(s)	1256, 1730
PvuII	CAGCTG	1 cut(s)	2396
RruI	TCGCGA	1 cut(s)	1999
RsaI	GTAC	6 cut(s)	411, 727, 1265, 1285, 2035, 2628
RsaNI	GTAC	6 cut(s)	410, 726, 1264, 1284, 2034, 2627
RseI	CAYNNNNRTG	1 cut(s)	1199
SapI	GCTCTTC	1 cut(s)	1071
SaqAI	TTAA	7 cut(s)	74, 651, 1182, 1293, 1458, 1907, 2010
SatI	GCNGC	6 cut(s)	1443, 1535, 1691, 1741, 2495, 2633
Sau96I	GGNCC	8 cut(s)	786, 817, 1120, 1270, 1874, 1946, 2044, 2514
SchI	GAGTC	4 cut(s)	135, 1895, 1994, 2467
ScrFI	CCNGG	5 cut(s)	38, 339, 414, 415, 1268
SduI	GDGCHC	1 cut(s)	162
SfaNI	GCATC	8 cut(s)	183, 498, 727, 749, 1060, 1232, 1327, 2065
SfcI	CTRYAG	2 cut(s)	1380, 2241
SfuI	TTCGAA	2 cut(s)	428, 2435
SinI	GGWCC	4 cut(s)	1120, 1270, 1946, 2514
SmaI	CCCGGG	1 cut(s)	415
SmiMI	CAYNNNNRTG	1 cut(s)	1199
SmlI	CTYRAG	3 cut(s)	685, 1181, 2499
SmoI	CTYRAG	3 cut(s)	685, 1181, 2499
SphI	GCATGC	1 cut(s)	2060
Sse9I	AATT	9 cut(s)	538, 652, 1234, 1294, 1764, 1897, 1904, 2014, 2289
SseBI	AGGCCT	2 cut(s)	1927, 2587
SspMI	CTAG	9 cut(s)	138, 570, 1053, 1260, 1371, 1737, 2019, 2031, 2141
StuI	AGGCCT	2 cut(s)	1927, 2587
StyD4I	CCNGG	5 cut(s)	36, 337, 412, 413, 1266
StyI	CCWWGG	3 cut(s)	56, 2067, 2357
TaaI	ACNGT	5 cut(s)	84, 1506, 2117, 2235, 2561
TaiI	ACGT	3 cut(s)	961, 1851, 2102
TaqI	TCGA	5 cut(s)	304, 314, 428, 1983, 2435
TasI	AATT	9 cut(s)	538, 652, 1234, 1294, 1764, 1897, 1904, 2014, 2289
TatI	WGTACW	2 cut(s)	725, 2626
TauI	GCSGC	1 cut(s)	2635
TfiI	GAWTC	4 cut(s)	19, 334, 2281, 2617
Tru1I	TTAA	7 cut(s)	74, 651, 1182, 1293, 1458, 1907, 2010
Tru9I	TTAA	7 cut(s)	74, 651, 1182, 1293, 1458, 1907, 2010
TscAI	CASTG	2 cut(s)	1566, 2238
TseFI	GTSAC	4 cut(s)	1136, 1887, 1986, 2542
TseI	GCWGC	5 cut(s)	1442, 1534, 1690, 1740, 2494
Tsp45I	GTSAC	4 cut(s)	1136, 1887, 1986, 2542
TspDTI	ATGAA	9 cut(s)	813, 1122, 1542, 1557, 1818, 1944, 2399, 2457, 2547
TspGWI	ACGGA	2 cut(s)	762, 1211
TspMI	CCCGGG	1 cut(s)	413
TspRI	CASTG	2 cut(s)	1566, 2238
Vha464I	CTTAAG	1 cut(s)	1181
VpaK11BI	GGWCC	4 cut(s)	1120, 1270, 1946, 2514
VspI	ATTAAT	2 cut(s)	74, 1458
XapI	RAATTY	1 cut(s)	2289
XceI	RCATGY	1 cut(s)	2060
XmaI	CCCGGG	1 cut(s)	413
XmiI	GTMKAC	2 cut(s)	7, 1594
XspI	CTAG	9 cut(s)	138, 570, 1053, 1260, 1371, 1737, 2019, 2031, 2141
Zsp2I	ATGCAT	3 cut(s)	742, 1225, 2058

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.