MD12G1188200.v1.1

Elongation factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0008799
Physical Location & Seq
Forward (+)
27016073 .. 27019163
3091 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1188200.v1.1.491

Sequence Viewer

Length: 729 bp
ATGGCGGGAATGGCTACCTCCTTCAACGTCTCCTCCGCCTCCTCCATCCTCAGCCGTCCGTCGCAGCTTTCGTCGCTTTCGTTCTCGTCCAAGCCGTTCTTTCTTGCCATGCGGAGTTCTTCCCGAACCCCCCTGCGTCTCGGATTTCCCAGGATAATGGCTTTTTCCAGCAACGATATAAAAGTGGGTTCCAACATTGAAGTTGATGGGGCTCCTTGGCGAGTTATAGAGTTTCTTCACGTGAAACCTGGGAAAGGGGCCGCATTTGTAAGGACCAAAATGCGCAATTATGTATCAGGAAACACTGTTGAGAAAACATTTCGAGCTGGCAGCACGATTAATGAGGCAAATATATACAAGGAAACAAAGCAATTCGTATACAAAGACGGTCCCCAGTTTGTCTTCATGGACCTGACTACCTATGAAGAAACTCGTGTAAATGAATCAAGTATTGGGGACAAAACAAAGTGGCTGAAAGAGGGCATGGACTGCAATTTGCTTCTTTGGAATGATAAACTTATCGACGTTGATATCCCCATCACAGTGCAGCTGAAGGTGGTTGATGTTGATCCCGGACTTAAAGGTGACACAGCTCAAGGAGGTGGAACAAAGCCTGCAACTCTTGAGACCGATGCTGTTGTCTTTGTCCCGCTGTTCGTTAATGTAGGTGATGAAATACTGGTGGATACCAGAACCGGGCAATACATGACCCGAGCTGAGAAAAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EFP EFP_N Elong-fact-P_C

Protein Analysis

243

Amino Acids

26.71

Weight (kDa)

8.56

Isoelectric Point (pI)

33.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EFP_N PF08207 56 - 112 2.2e-25 Elongation factor P (EF-P) KOW-like domain
EFP PF01132 124 - 174 1.5e-19 Elongation factor P (EF-P) OB domain
Elong-fact-P_C PF09285 182 - 238 8.8e-24 Elongation factor P, C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 284
AccI GTMKAC 1 cut(s) 378
AciI CCGC 5 cut(s) 5, 36, 112, 261, 650
AclWI GGATC 1 cut(s) 563
AcuI CTGAAG 1 cut(s) 572
AcvI CACGTG 1 cut(s) 241
AfiI CCNNNNNNNGG 2 cut(s) 254, 696
AgsI TTSAA 2 cut(s) 25, 200
AjnI CCWGG 2 cut(s) 149, 247
AjuI GAANNNNNNNTTGG 2 cut(s) 435, 467
AluBI AGCT 5 cut(s) 67, 326, 550, 593, 716
AluI AGCT 5 cut(s) 67, 326, 550, 593, 716
Alw26I GTCTC 3 cut(s) 34, 143, 620
AlwI GGATC 1 cut(s) 563
Ama87I CYCGRG 1 cut(s) 711
AoxI GGCC 1 cut(s) 258
ApeKI GCWGC 3 cut(s) 64, 330, 547
AseI ATTAAT 1 cut(s) 339
AspLEI GCGC 1 cut(s) 285
AspS9I GGNCC 4 cut(s) 258, 273, 389, 409
AsuC2I CCSGG 2 cut(s) 573, 697
AsuHPI GGTGA 2 cut(s) 596, 680
AvaI CYCGRG 1 cut(s) 711
AvaII GGWCC 3 cut(s) 273, 389, 409
BanII GRGCYC 1 cut(s) 214
BauI CACGAG 1 cut(s) 432
BbrPI CACGTG 1 cut(s) 241
BbsI GAAGAC 1 cut(s) 394
BbvCI CCTCAGC 1 cut(s) 50
BbvI GCAGC 3 cut(s) 76, 342, 559
BccI CCATC 3 cut(s) 53, 200, 545
BceAI ACGGC 2 cut(s) 39, 79
BciT130I CCWGG 2 cut(s) 151, 249
BciVI GTATCC 1 cut(s) 679
BcnI CCSGG 2 cut(s) 573, 697
BcoDI GTCTC 3 cut(s) 34, 143, 620
BfuI GTATCC 1 cut(s) 679
BisI GCNGC 4 cut(s) 65, 261, 331, 548
BlsI GCNGC 4 cut(s) 66, 262, 332, 549
Bme1390I CCNGG 4 cut(s) 151, 249, 573, 697
Bme18I GGWCC 3 cut(s) 273, 389, 409
BmeT110I CYCGRG 1 cut(s) 711
BmgT120I GGNCC 4 cut(s) 258, 273, 389, 409
BmiI GGNNCC 4 cut(s) 190, 213, 259, 391
BmrFI CCNGG 4 cut(s) 151, 249, 573, 697
BmrI ACTGGG 1 cut(s) 388
BmsI GCATC 1 cut(s) 622
BmuI ACTGGG 1 cut(s) 388
BpiI GAAGAC 1 cut(s) 394
Bpu10I CCTNAGC 1 cut(s) 50
BpuEI CTTGAG 2 cut(s) 579, 644
BpuMI CCSGG 2 cut(s) 573, 697
BsaAI YACGTR 1 cut(s) 241
BsaBI GATNNNNATC 1 cut(s) 567
BsaI GGTCTC 1 cut(s) 620
BsaJI CCNNGG 3 cut(s) 149, 215, 248
BsaXI ACNNNNNCTCC 2 cut(s) 17, 47
Bsc4I CCNNNNNNNGG 2 cut(s) 254, 696
Bse1I ACTGG 2 cut(s) 394, 684
Bse8I GATNNNNATC 1 cut(s) 567
BseBI CCWGG 2 cut(s) 151, 249
BseDI CCNNGG 3 cut(s) 149, 215, 248
BseGI GGATG 1 cut(s) 45
BseJI GATNNNNATC 1 cut(s) 567
BseLI CCNNNNNNNGG 2 cut(s) 254, 696
BseMII CTCAG 2 cut(s) 64, 708
BseNI ACTGG 2 cut(s) 394, 684
BseRI GAGGAG 2 cut(s) 22, 31
BseXI GCAGC 3 cut(s) 76, 342, 559
BsgI GTGCAG 1 cut(s) 566
BshFI GGCC 1 cut(s) 260
BsiHKCI CYCGRG 1 cut(s) 711
BsiSI CCGG 2 cut(s) 573, 696
BslFI GGGAC 3 cut(s) 375, 470, 632
BslI CCNNNNNNNGG 2 cut(s) 254, 696
BsmAI GTCTC 3 cut(s) 34, 143, 620
BsmBI CGTCTC 2 cut(s) 34, 143
BsmFI GGGAC 3 cut(s) 375, 470, 632
BsnI GGCC 1 cut(s) 260
Bso31I GGTCTC 1 cut(s) 620
BsoBI CYCGRG 1 cut(s) 711
Bsp1286I GDGCHC 1 cut(s) 214
Bsp143I GATC 1 cut(s) 568
BspACI CCGC 5 cut(s) 5, 36, 112, 261, 650
BspANI GGCC 1 cut(s) 260
BspCNI CTCAG 2 cut(s) 63, 709
BspLI GGNNCC 4 cut(s) 190, 213, 259, 391
BspPI GGATC 1 cut(s) 563
BspTNI GGTCTC 1 cut(s) 620
BsrI ACTGG 2 cut(s) 394, 684
BssECI CCNNGG 3 cut(s) 149, 215, 248
BssMI GATC 1 cut(s) 568
BssNAI GTATAC 1 cut(s) 379
BssSI CACGAG 1 cut(s) 432
BssT1I CCWWGG 1 cut(s) 215
Bst1107I GTATAC 1 cut(s) 379
Bst2BI CACGAG 1 cut(s) 432
Bst2UI CCWGG 2 cut(s) 151, 249
Bst4CI ACNGT 3 cut(s) 307, 389, 544
BstAPI GCANNNNNTGC 1 cut(s) 489
BstBAI YACGTR 1 cut(s) 241
BstC8I GCNNGC 2 cut(s) 328, 615
BstDEI CTNAG 2 cut(s) 50, 717
BstENI CCTNNNNNAGG 1 cut(s) 252
BstF5I GGATG 1 cut(s) 45
BstHHI GCGC 1 cut(s) 285
BstKTI GATC 1 cut(s) 571
BstMAI GTCTC 3 cut(s) 34, 143, 620
BstMBI GATC 1 cut(s) 568
BstMWI GCNNNNNNNGC 3 cut(s) 11, 73, 489
BstNI CCWGG 2 cut(s) 151, 249
BstSCI CCNGG 4 cut(s) 149, 247, 571, 695
BstV1I GCAGC 3 cut(s) 76, 342, 559
BstV2I GAAGAC 1 cut(s) 394
BstXI CCANNNNNNTGG 1 cut(s) 157
BstZ17I GTATAC 1 cut(s) 379
BsuI GTATCC 1 cut(s) 679
BsuRI GGCC 1 cut(s) 260
BtsCI GGATG 1 cut(s) 45
BtsIMutI CAGTG 2 cut(s) 303, 549
Cac8I GCNNGC 2 cut(s) 328, 615
CfoI GCGC 1 cut(s) 285
Cfr13I GGNCC 4 cut(s) 258, 273, 389, 409
CseI GACGC 1 cut(s) 125
CviAII CATG 4 cut(s) 109, 406, 484, 706
DdeI CTNAG 2 cut(s) 50, 717
DpnI GATC 1 cut(s) 570
DpnII GATC 1 cut(s) 568
EciI GGCGGA 1 cut(s) 25
Eco130I CCWWGG 1 cut(s) 215
Eco24I GRGCYC 1 cut(s) 214
Eco31I GGTCTC 1 cut(s) 620
Eco32I GATATC 1 cut(s) 532
Eco47I GGWCC 3 cut(s) 273, 389, 409
Eco57I CTGAAG 1 cut(s) 572
Eco72I CACGTG 1 cut(s) 241
Eco88I CYCGRG 1 cut(s) 711
EcoNI CCTNNNNNAGG 1 cut(s) 252
EcoRII CCWGG 2 cut(s) 149, 247
EcoRV GATATC 1 cut(s) 532
EcoT14I CCWWGG 1 cut(s) 215
EcoT38I GRGCYC 1 cut(s) 214
ErhI CCWWGG 1 cut(s) 215
Esp3I CGTCTC 2 cut(s) 34, 143
FaeI CATG 4 cut(s) 112, 409, 487, 709
FalI AAGNNNNNCTT 2 cut(s) 83, 115
FaqI GGGAC 3 cut(s) 375, 470, 632
FatI CATG 4 cut(s) 108, 405, 483, 705
FauI CCCGC 1 cut(s) 657
FblI GTMKAC 1 cut(s) 378
Fnu4HI GCNGC 4 cut(s) 65, 261, 331, 548
FokI GGATG 1 cut(s) 32
FriOI GRGCYC 1 cut(s) 214
Fsp4HI GCNGC 4 cut(s) 65, 261, 331, 548
FspI TGCGCA 1 cut(s) 284
GlaI GCGC 1 cut(s) 284
GluI GCNGC 4 cut(s) 65, 261, 331, 548
HaeIII GGCC 1 cut(s) 260
HapII CCGG 2 cut(s) 573, 696
HgaI GACGC 1 cut(s) 125
HhaI GCGC 1 cut(s) 285
Hin1II CATG 4 cut(s) 112, 409, 487, 709
Hin6I GCGC 1 cut(s) 283
HinP1I GCGC 1 cut(s) 283
HinfI GANTC 1 cut(s) 443
HpaII CCGG 2 cut(s) 573, 696
HphI GGTGA 2 cut(s) 596, 680
Hpy166II GTNNAC 1 cut(s) 379
Hpy188I TCNGA 1 cut(s) 143
Hpy188III TCNNGA 3 cut(s) 123, 297, 623
Hpy8I GTNNAC 1 cut(s) 379
Hpy99I CGWCG 3 cut(s) 64, 76, 527
HpyAV CCTTC 2 cut(s) 31, 547
HpyCH4III ACNGT 3 cut(s) 307, 389, 544
HpyCH4IV ACGT 3 cut(s) 27, 240, 525
HpyCH4V TGCA 3 cut(s) 492, 547, 617
HpyF10VI GCNNNNNNNGC 3 cut(s) 11, 73, 489
HpyF3I CTNAG 2 cut(s) 50, 717
HpySE526I ACGT 3 cut(s) 27, 240, 525
Hsp92II CATG 4 cut(s) 112, 409, 487, 709
HspAI GCGC 1 cut(s) 283
Kzo9I GATC 1 cut(s) 568
LmnI GCTCC 1 cut(s) 217
Lsp1109I GCAGC 3 cut(s) 76, 342, 559
LweI GCATC 1 cut(s) 622
MaeII ACGT 3 cut(s) 27, 240, 525
MaeIII GTNAC 1 cut(s) 584
MalI GATC 1 cut(s) 570
MboI GATC 1 cut(s) 568
MboII GAAGA 4 cut(s) 111, 227, 394, 437
MhlI GDGCHC 1 cut(s) 214
MluCI AATT 4 cut(s) 286, 371, 493, 724
MmeI TCCRAC 1 cut(s) 216
MnlI CCTC 8 cut(s) 28, 43, 49, 52, 59, 337, 472, 593
MseI TTAA 3 cut(s) 339, 579, 660
MslI CAYNNNNRTG 1 cut(s) 542
MspA1I CMGCKG 2 cut(s) 550, 652
MspI CCGG 2 cut(s) 573, 696
MspR9I CCNGG 4 cut(s) 151, 249, 573, 697
MvaI CCWGG 2 cut(s) 151, 249
MwoI GCNNNNNNNGC 3 cut(s) 11, 73, 489
NciI CCSGG 2 cut(s) 573, 697
NdeII GATC 1 cut(s) 568
NlaIII CATG 4 cut(s) 112, 409, 487, 709
NlaIV GGNNCC 4 cut(s) 190, 213, 259, 391
NmuCI GTSAC 1 cut(s) 584
NsbI TGCGCA 1 cut(s) 284
PcsI WCGNNNNNNNCGW 3 cut(s) 68, 77, 92
PfeI GAWTC 1 cut(s) 443
PfoI TCCNGGA 1 cut(s) 571
PkrI GCNGC 4 cut(s) 66, 262, 332, 549
PmaCI CACGTG 1 cut(s) 241
PmlI CACGTG 1 cut(s) 241
Ppu21I YACGTR 1 cut(s) 241
PshBI ATTAAT 1 cut(s) 339
Psp6I CCWGG 2 cut(s) 149, 247
PspCI CACGTG 1 cut(s) 241
PspGI CCWGG 2 cut(s) 149, 247
PspN4I GGNNCC 4 cut(s) 190, 213, 259, 391
PspPI GGNCC 4 cut(s) 258, 273, 389, 409
PvuII CAGCTG 1 cut(s) 550
RseI CAYNNNNRTG 1 cut(s) 542
SaqAI TTAA 3 cut(s) 339, 579, 660
SatI GCNGC 4 cut(s) 65, 261, 331, 548
Sau3AI GATC 1 cut(s) 568
Sau96I GGNCC 4 cut(s) 258, 273, 389, 409
ScrFI CCNGG 4 cut(s) 151, 249, 573, 697
SduI GDGCHC 1 cut(s) 214
SfaNI GCATC 1 cut(s) 622
SinI GGWCC 3 cut(s) 273, 389, 409
SmiMI CAYNNNNRTG 1 cut(s) 542
SmlI CTYRAG 2 cut(s) 594, 623
SmoI CTYRAG 2 cut(s) 594, 623
Sse9I AATT 4 cut(s) 286, 371, 493, 724
SsiI CCGC 5 cut(s) 5, 36, 112, 261, 650
StyD4I CCNGG 4 cut(s) 149, 247, 571, 695
StyI CCWWGG 1 cut(s) 215
TaaI ACNGT 3 cut(s) 307, 389, 544
TaiI ACGT 3 cut(s) 30, 243, 528
TaqI TCGA 2 cut(s) 322, 522
TaqII GACCGA 1 cut(s) 644
TasI AATT 4 cut(s) 286, 371, 493, 724
TauI GCSGC 1 cut(s) 263
TfiI GAWTC 1 cut(s) 443
Tru1I TTAA 3 cut(s) 339, 579, 660
Tru9I TTAA 3 cut(s) 339, 579, 660
TscAI CASTG 2 cut(s) 310, 549
TseFI GTSAC 1 cut(s) 584
TseI GCWGC 3 cut(s) 64, 330, 547
Tsp45I GTSAC 1 cut(s) 584
TspDTI ATGAA 4 cut(s) 394, 438, 456, 687
TspGWI ACGGA 1 cut(s) 48
TspRI CASTG 2 cut(s) 310, 549
VpaK11BI GGWCC 3 cut(s) 273, 389, 409
VspI ATTAAT 1 cut(s) 339
XagI CCTNNNNNAGG 1 cut(s) 252
XmiI GTMKAC 1 cut(s) 378
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.