MD12G1204500.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0013695
Physical Location & Seq
Reverse (-)
28501154 .. 28505722
4569 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1204500.v1.1.491

Sequence Viewer

Length: 555 bp
ATGGATGAACTAGGTTCTCGAGAAAGTGATCTGGATTTTGACTTGGAAAGTGGTGACACTGAAAGCTCCGATGAAGAAGTACATGAAGAGTCAAGTTCAGATGGTAAGCGTCCCAGGAAAACAATGGCTAGGGCAAGAAGTGGATACTTGTATGTTGATGGATCAATAGCAGCTAGTGATGGACTAAGCACAAGCAAGAATGTTTCGAGTATTGGTGAGAATATGGATATGTTAGGTAGTAGCACGGGGAAAATGACAGAAGGAGGAGTGGAGAATAGAATGGGAGATAAACAGAAAAAGAAGATCCCTAAGAAGCATCCCAAGCCACCTCGGCCTCCTACAGGGCCAGCATTACATGCAGCTGATATAGAATTGGTCAACGAAATTTCAAAGCGTGCGAGGTTGCGGCGTGCAAGGAGAGAGAGAATGAATGCATTGAAGAGAATGAAAGCTGATAAAACCTCGTCTTCAAAGATCAACTTTTTGGCAATGGTTGTAACCGCTATCTTCTTCTTTGTGATAATCTTTCAAGGAATCTTGGGTTCTCGTGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

185

Amino Acids

20.25

Weight (kDa)

9.45

Isoelectric Point (pI)

47.52

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013695)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g07660 FvH4_6g07660
malus_domestica MD12G1204500.v1.1
prunus_persica Prupe.6G311200_v2.0.a1 Prupe.6G311200_v2.0.a1 Prupe.6G311200_v2.0.a1
pyrus_communis pycom12g19190
rosa_chinensis RchiOBHm_Chr3g0457351
rosa_laevigata RLG00000025233
rosa_multiflora Rmu_sc0005442.1_g000009
rosa_roxburghii Rroxscaffold_6G00421960
rosa_rugosa Rorug03G0019900 Rorug03G0020000
rosa_samantha Rh3AG080200 Rh3BG082000 Rh3CG082300 Rh3DG083700
rosa_wichuraiana Rw3G006900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 406, 501
AclWI GGATC 2 cut(s) 169, 298
AcsI RAATTY 1 cut(s) 384
AfaI GTAC 1 cut(s) 81
AfiI CCNNNNNNNGG 1 cut(s) 341
AgsI TTSAA 4 cut(s) 390, 439, 471, 530
AjnI CCWGG 1 cut(s) 113
AluBI AGCT 4 cut(s) 66, 173, 362, 452
AluI AGCT 4 cut(s) 66, 173, 362, 452
AlwI GGATC 2 cut(s) 169, 298
Ama87I CYCGRG 1 cut(s) 18
AoxI GGCC 2 cut(s) 332, 344
ApeKI GCWGC 2 cut(s) 170, 359
ApoI RAATTY 1 cut(s) 384
AspS9I GGNCC 1 cut(s) 344
AsuHPI GGTGA 2 cut(s) 65, 227
AvaI CYCGRG 1 cut(s) 18
BauI CACGAG 1 cut(s) 546
BbsI GAAGAC 1 cut(s) 459
BbvI GCAGC 2 cut(s) 182, 371
BccI CCATC 3 cut(s) 95, 152, 173
BciT130I CCWGG 1 cut(s) 115
BciVI GTATCC 1 cut(s) 137
BfaI CTAG 3 cut(s) 11, 129, 174
BfmI CTRYAG 1 cut(s) 339
BfuI GTATCC 1 cut(s) 137
BglI GCCNNNNNGGC 1 cut(s) 331
BisI GCNGC 3 cut(s) 171, 360, 407
BlsI GCNGC 3 cut(s) 172, 361, 408
Bme1390I CCNGG 1 cut(s) 115
BmeT110I CYCGRG 1 cut(s) 18
BmgT120I GGNCC 1 cut(s) 344
BmrFI CCNGG 1 cut(s) 115
BmsI GCATC 1 cut(s) 325
BpiI GAAGAC 1 cut(s) 459
BsaJI CCNNGG 2 cut(s) 113, 329
Bsc4I CCNNNNNNNGG 1 cut(s) 341
Bse3DI GCAATG 1 cut(s) 495
BseBI CCWGG 1 cut(s) 115
BseDI CCNNGG 2 cut(s) 113, 329
BseGI GGATG 2 cut(s) 10, 316
BseLI CCNNNNNNNGG 1 cut(s) 341
BseMI GCAATG 1 cut(s) 495
BseRI GAGGAG 1 cut(s) 279
BseXI GCAGC 2 cut(s) 182, 371
BshFI GGCC 2 cut(s) 334, 346
BsiHKCI CYCGRG 1 cut(s) 18
BslFI GGGAC 1 cut(s) 96
BslI CCNNNNNNNGG 1 cut(s) 341
BsmFI GGGAC 1 cut(s) 96
BsmI GAATGC 1 cut(s) 436
BsnI GGCC 2 cut(s) 334, 346
BsoBI CYCGRG 1 cut(s) 18
Bsp143I GATC 4 cut(s) 28, 161, 303, 474
BspACI CCGC 2 cut(s) 406, 501
BspANI GGCC 2 cut(s) 334, 346
BspPI GGATC 2 cut(s) 169, 298
BsrDI GCAATG 1 cut(s) 495
BssECI CCNNGG 2 cut(s) 113, 329
BssMI GATC 4 cut(s) 28, 161, 303, 474
BssSI CACGAG 1 cut(s) 546
Bst2BI CACGAG 1 cut(s) 546
Bst2UI CCWGG 1 cut(s) 115
Bst6I CTCTTC 2 cut(s) 81, 434
BstAPI GCANNNNNTGC 1 cut(s) 356
BstC8I GCNNGC 3 cut(s) 348, 396, 411
BstDEI CTNAG 2 cut(s) 185, 309
BstENI CCTNNNNNAGG 1 cut(s) 339
BstF5I GGATG 2 cut(s) 10, 316
BstKTI GATC 4 cut(s) 31, 164, 306, 477
BstMBI GATC 4 cut(s) 28, 161, 303, 474
BstMWI GCNNNNNNNGC 3 cut(s) 322, 331, 356
BstNI CCWGG 1 cut(s) 115
BstNSI RCATGY 1 cut(s) 359
BstSCI CCNGG 1 cut(s) 113
BstSFI CTRYAG 1 cut(s) 339
BstV1I GCAGC 2 cut(s) 182, 371
BstV2I GAAGAC 1 cut(s) 459
BstX2I RGATCY 1 cut(s) 303
BstYI RGATCY 1 cut(s) 303
BsuI GTATCC 1 cut(s) 137
BsuRI GGCC 2 cut(s) 334, 346
BtsCI GGATG 2 cut(s) 10, 316
BtsIMutI CAGTG 1 cut(s) 57
Cac8I GCNNGC 3 cut(s) 348, 396, 411
Cfr13I GGNCC 1 cut(s) 344
CseI GACGC 1 cut(s) 98
Csp6I GTAC 1 cut(s) 80
CviAII CATG 3 cut(s) 83, 356, 552
CviJI RGCY 8 cut(s) 66, 128, 173, 325, 334, 346, 362, 452
CviKI_1 RGCY 8 cut(s) 66, 128, 173, 325, 334, 346, 362, 452
CviQI GTAC 1 cut(s) 80
DdeI CTNAG 2 cut(s) 185, 309
DpnI GATC 4 cut(s) 30, 163, 305, 476
DpnII GATC 4 cut(s) 28, 161, 303, 474
Eam1104I CTCTTC 2 cut(s) 81, 434
EarI CTCTTC 2 cut(s) 81, 434
Eco88I CYCGRG 1 cut(s) 18
EcoNI CCTNNNNNAGG 1 cut(s) 339
EcoRII CCWGG 1 cut(s) 113
EcoT22I ATGCAT 1 cut(s) 436
FaeI CATG 3 cut(s) 86, 359, 555
FaiI YATR 7 cut(s) 84, 153, 224, 230, 357, 368, 553
FalI AAGNNNNNCTT 2 cut(s) 464, 496
FaqI GGGAC 1 cut(s) 96
FatI CATG 3 cut(s) 82, 355, 551
Fnu4HI GCNGC 3 cut(s) 171, 360, 407
FokI GGATG 2 cut(s) 17, 303
Fsp4HI GCNGC 3 cut(s) 171, 360, 407
FspBI CTAG 3 cut(s) 11, 129, 174
GluI GCNGC 3 cut(s) 171, 360, 407
HaeIII GGCC 2 cut(s) 334, 346
HgaI GACGC 1 cut(s) 98
Hin1II CATG 3 cut(s) 86, 359, 555
HincII GTYRAC 1 cut(s) 379
HindII GTYRAC 1 cut(s) 379
HinfI GANTC 2 cut(s) 89, 534
HphI GGTGA 2 cut(s) 65, 227
Hpy166II GTNNAC 1 cut(s) 379
Hpy188I TCNGA 2 cut(s) 70, 100
Hpy188III TCNNGA 3 cut(s) 18, 20, 32
Hpy8I GTNNAC 1 cut(s) 379
HpyAV CCTTC 1 cut(s) 254
HpyCH4V TGCA 4 cut(s) 359, 413, 434, 551
HpyF10VI GCNNNNNNNGC 3 cut(s) 322, 331, 356
HpyF3I CTNAG 2 cut(s) 185, 309
Hsp92II CATG 3 cut(s) 86, 359, 555
Kzo9I GATC 4 cut(s) 28, 161, 303, 474
LmnI GCTCC 1 cut(s) 71
LpnPI CCDG 5 cut(s) 17, 100, 127, 327, 360
Lsp1109I GCAGC 2 cut(s) 182, 371
LweI GCATC 1 cut(s) 325
MaeI CTAG 3 cut(s) 11, 129, 174
MaeIII GTNAC 2 cut(s) 53, 496
MalI GATC 4 cut(s) 30, 163, 305, 476
MboI GATC 4 cut(s) 28, 161, 303, 474
MboII GAAGA 7 cut(s) 86, 98, 313, 451, 459, 499, 502
MflI RGATCY 1 cut(s) 303
MluCI AATT 2 cut(s) 371, 384
MlyI GAGTC 1 cut(s) 98
MnlI CCTC 5 cut(s) 257, 339, 345, 393, 472
Mph1103I ATGCAT 1 cut(s) 436
MspA1I CMGCKG 1 cut(s) 362
MspR9I CCNGG 1 cut(s) 115
Mva1269I GAATGC 1 cut(s) 436
MvaI CCWGG 1 cut(s) 115
MwoI GCNNNNNNNGC 3 cut(s) 322, 331, 356
NdeII GATC 4 cut(s) 28, 161, 303, 474
NlaIII CATG 3 cut(s) 86, 359, 555
NmeAIII GCCGAG 1 cut(s) 310
NmuCI GTSAC 1 cut(s) 53
NsiI ATGCAT 1 cut(s) 436
NspI RCATGY 1 cut(s) 359
PaeR7I CTCGAG 1 cut(s) 18
PctI GAATGC 1 cut(s) 436
PfeI GAWTC 1 cut(s) 534
PkrI GCNGC 3 cut(s) 172, 361, 408
PleI GAGTC 1 cut(s) 97
PpsI GAGTC 1 cut(s) 97
Psp6I CCWGG 1 cut(s) 113
PspGI CCWGG 1 cut(s) 113
PspPI GGNCC 1 cut(s) 344
PsuI RGATCY 1 cut(s) 303
PvuII CAGCTG 1 cut(s) 362
RsaI GTAC 1 cut(s) 81
RsaNI GTAC 1 cut(s) 80
SatI GCNGC 3 cut(s) 171, 360, 407
Sau3AI GATC 4 cut(s) 28, 161, 303, 474
Sau96I GGNCC 1 cut(s) 344
SchI GAGTC 1 cut(s) 98
ScrFI CCNGG 1 cut(s) 115
SetI ASST 9 cut(s) 16, 68, 175, 238, 331, 364, 404, 454, 464
SfaNI GCATC 1 cut(s) 325
SfcI CTRYAG 1 cut(s) 339
Sfr274I CTCGAG 1 cut(s) 18
SlaI CTCGAG 1 cut(s) 18
SmlI CTYRAG 1 cut(s) 18
SmoI CTYRAG 1 cut(s) 18
Sse9I AATT 2 cut(s) 371, 384
SsiI CCGC 2 cut(s) 406, 501
SspMI CTAG 3 cut(s) 11, 129, 174
StyD4I CCNGG 1 cut(s) 113
TaqI TCGA 2 cut(s) 19, 206
TasI AATT 2 cut(s) 371, 384
TatI WGTACW 1 cut(s) 79
TauI GCSGC 1 cut(s) 409
TfiI GAWTC 1 cut(s) 534
TscAI CASTG 1 cut(s) 64
TseFI GTSAC 1 cut(s) 53
TseI GCWGC 2 cut(s) 170, 359
Tsp45I GTSAC 1 cut(s) 53
TspDTI ATGAA 5 cut(s) 21, 87, 99, 443, 461
TspRI CASTG 1 cut(s) 64
XagI CCTNNNNNAGG 1 cut(s) 339
XapI RAATTY 1 cut(s) 384
XceI RCATGY 1 cut(s) 359
XcmI CCANNNNNNNNNTGG 1 cut(s) 121
XhoI CTCGAG 1 cut(s) 18
XspI CTAG 3 cut(s) 11, 129, 174
Zsp2I ATGCAT 1 cut(s) 436
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.