RLG00000025233

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0013695
Physical Location & Seq
Forward (+)
43219890 .. 43222230
2341 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000025233

Sequence Viewer

Length: 747 bp
ATGGATCATATAGGTTCTAGAGAATGTGATCTGGAGTTTGACTTGGAAAGTGGTGAGACTGAATCCAACAGTGAAGAAGATGGAAGTGAAGAGTCCCGTTCCCATGGTAAACATTTCAATAAATTATTGAATAGGGGAAGAAGTGGATACTTGTGTATTGAAGGATCAATAACAGCTAATGATGTTGTAGAAAGCAATAAGGTATCAAATATTGATGAGACTAGGAATGTGATAGAAGGAGGAATTGAGAAGAGAACGGTGGATAAACAGAAAATGAAAGGCTCTGAGAAGCATCCCAAGCCACCTAGGCCTCCTACGGGTCCATCATTACATGCAGCAGATATAGAGTTGGTTAAAGAAATTTCAAAGCATACTAGGTTGCGGCGTGCAAGGAGAGAAAGACTGAAAGCATTGAAGAATATGAAAACTGATAGAAGTCATAGAACATCATCAGCAAATATCAACTTTTTGGCAATAATCATCACAGCTATCTTCTTCTTTGTGATAATCTTCCAAGGTATGAATAAGGGCAAAGTGCACAGATACGAAGCAAACTCTTTACTGCTGTTATATTCCATGTTCAATGTTGGATTACCTCCAGTATGTGTAATGAGGCATCCCAAGGTTATATTCAGTGTTCAATGCTGGATTGCCTCCACAAGGTTTATTTCTAGTATTGATGTGATCGAATGTGTTGGGCATGACAGGGGAGAGGGATGCAGGGGGCCAGTGCCAACTGGCAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

249

Amino Acids

27.86

Weight (kDa)

9.01

Isoelectric Point (pI)

55.45

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013695)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g07660 FvH4_6g07660
malus_domestica MD12G1204500.v1.1
prunus_persica Prupe.6G311200_v2.0.a1 Prupe.6G311200_v2.0.a1 Prupe.6G311200_v2.0.a1
pyrus_communis pycom12g19190
rosa_chinensis RchiOBHm_Chr3g0457351
rosa_laevigata RLG00000025233
rosa_multiflora Rmu_sc0005442.1_g000009
rosa_roxburghii Rroxscaffold_6G00421960
rosa_rugosa Rorug03G0019900 Rorug03G0020000
rosa_samantha Rh3AG080200 Rh3BG082000 Rh3CG082300 Rh3DG083700
rosa_wichuraiana Rw3G006900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 382
AclWI GGATC 2 cut(s) 12, 172
AcsI RAATTY 1 cut(s) 360
AfiI CCNNNNNNNGG 2 cut(s) 317, 660
AgsI TTSAA 7 cut(s) 118, 130, 161, 366, 415, 583, 641
AjuI GAANNNNNNNTTGG 2 cut(s) 614, 646
AluBI AGCT 2 cut(s) 176, 488
AluI AGCT 2 cut(s) 176, 488
Alw21I GWGCWC 1 cut(s) 540
Alw26I GTCTC 2 cut(s) 50, 212
Alw44I GTGCAC 1 cut(s) 536
AlwI GGATC 2 cut(s) 12, 172
AoxI GGCC 2 cut(s) 308, 725
ApaLI GTGCAC 1 cut(s) 536
ApeKI GCWGC 1 cut(s) 335
ApoI RAATTY 1 cut(s) 360
AspA2I CCTAGG 1 cut(s) 305
AspS9I GGNCC 2 cut(s) 320, 725
AsuHPI GGTGA 1 cut(s) 65
AvaII GGWCC 1 cut(s) 320
AvrII CCTAGG 1 cut(s) 305
BaeGI GKGCMC 1 cut(s) 540
BaeI ACNNNNGTAYC 2 cut(s) 139, 172
Bbv12I GWGCWC 1 cut(s) 540
BbvI GCAGC 1 cut(s) 347
BccI CCATC 2 cut(s) 74, 331
BciVI GTATCC 1 cut(s) 140
BcoDI GTCTC 2 cut(s) 50, 212
BfaI CTAG 5 cut(s) 18, 222, 306, 375, 672
BfuI GTATCC 1 cut(s) 140
BglI GCCNNNNNGGC 1 cut(s) 307
BisI GCNGC 2 cut(s) 336, 383
BlnI CCTAGG 1 cut(s) 305
BlsI GCNGC 2 cut(s) 337, 384
Bme18I GGWCC 1 cut(s) 320
BmgT120I GGNCC 2 cut(s) 320, 725
BmiI GGNNCC 2 cut(s) 321, 726
BmsI GCATC 3 cut(s) 301, 625, 707
BpmI CTGGAG 2 cut(s) 53, 582
BsaJI CCNNGG 4 cut(s) 103, 305, 514, 621
Bsc4I CCNNNNNNNGG 2 cut(s) 317, 660
Bse1I ACTGG 3 cut(s) 599, 728, 742
BseDI CCNNGG 4 cut(s) 103, 305, 514, 621
BseGI GGATG 3 cut(s) 292, 616, 722
BseLI CCNNNNNNNGG 2 cut(s) 317, 660
BseMII CTCAG 1 cut(s) 276
BseNI ACTGG 3 cut(s) 599, 728, 742
BseSI GKGCMC 1 cut(s) 540
BseXI GCAGC 1 cut(s) 347
BshFI GGCC 2 cut(s) 310, 727
BsiHKAI GWGCWC 1 cut(s) 540
BslFI GGGAC 1 cut(s) 79
BslI CCNNNNNNNGG 2 cut(s) 317, 660
BsmAI GTCTC 2 cut(s) 50, 212
BsmFI GGGAC 1 cut(s) 79
BsnI GGCC 2 cut(s) 310, 727
Bsp1286I GDGCHC 1 cut(s) 540
Bsp143I GATC 4 cut(s) 4, 28, 164, 684
Bsp19I CCATGG 1 cut(s) 103
BspACI CCGC 1 cut(s) 382
BspANI GGCC 2 cut(s) 310, 727
BspCNI CTCAG 1 cut(s) 277
BspLI GGNNCC 2 cut(s) 321, 726
BspPI GGATC 2 cut(s) 12, 172
BsrI ACTGG 3 cut(s) 599, 728, 742
BssECI CCNNGG 4 cut(s) 103, 305, 514, 621
BssMI GATC 4 cut(s) 4, 28, 164, 684
BssT1I CCWWGG 4 cut(s) 103, 305, 514, 621
Bst4CI ACNGT 2 cut(s) 71, 259
Bst6I CTCTTC 2 cut(s) 84, 245
BstC8I GCNNGC 1 cut(s) 387
BstDEI CTNAG 1 cut(s) 285
BstDSI CCRYGG 1 cut(s) 103
BstENI CCTNNNNNAGG 1 cut(s) 658
BstF5I GGATG 3 cut(s) 292, 616, 722
BstKTI GATC 4 cut(s) 7, 31, 167, 687
BstMAI GTCTC 2 cut(s) 50, 212
BstMBI GATC 4 cut(s) 4, 28, 164, 684
BstMWI GCNNNNNNNGC 2 cut(s) 298, 307
BstNSI RCATGY 1 cut(s) 335
BstSLI GKGCMC 1 cut(s) 540
BstV1I GCAGC 1 cut(s) 347
BsuI GTATCC 1 cut(s) 140
BsuRI GGCC 2 cut(s) 310, 727
BtgI CCRYGG 1 cut(s) 103
BtsCI GGATG 3 cut(s) 292, 616, 722
BtsIMutI CAGTG 3 cut(s) 76, 640, 735
Cac8I GCNNGC 1 cut(s) 387
Cfr13I GGNCC 2 cut(s) 320, 725
CviAII CATG 4 cut(s) 104, 332, 577, 701
CviJI RGCY 6 cut(s) 176, 282, 301, 310, 488, 727
CviKI_1 RGCY 6 cut(s) 176, 282, 301, 310, 488, 727
DdeI CTNAG 1 cut(s) 285
DpnI GATC 4 cut(s) 6, 30, 166, 686
DpnII GATC 4 cut(s) 4, 28, 164, 684
Eam1104I CTCTTC 2 cut(s) 84, 245
EarI CTCTTC 2 cut(s) 84, 245
Eco130I CCWWGG 4 cut(s) 103, 305, 514, 621
Eco147I AGGCCT 1 cut(s) 310
Eco47I GGWCC 1 cut(s) 320
EcoNI CCTNNNNNAGG 1 cut(s) 658
EcoT14I CCWWGG 4 cut(s) 103, 305, 514, 621
ErhI CCWWGG 4 cut(s) 103, 305, 514, 621
FaeI CATG 4 cut(s) 107, 335, 580, 704
FaqI GGGAC 1 cut(s) 79
FatI CATG 4 cut(s) 103, 331, 576, 700
Fnu4HI GCNGC 2 cut(s) 336, 383
FokI GGATG 3 cut(s) 279, 603, 729
Fsp4HI GCNGC 2 cut(s) 336, 383
FspBI CTAG 5 cut(s) 18, 222, 306, 375, 672
GluI GCNGC 2 cut(s) 336, 383
GsuI CTGGAG 2 cut(s) 53, 582
HaeIII GGCC 2 cut(s) 310, 727
Hin1II CATG 4 cut(s) 107, 335, 580, 704
HinfI GANTC 2 cut(s) 62, 92
HphI GGTGA 1 cut(s) 65
Hpy166II GTNNAC 2 cut(s) 110, 538
Hpy188I TCNGA 1 cut(s) 286
Hpy188III TCNNGA 2 cut(s) 18, 32
Hpy8I GTNNAC 2 cut(s) 110, 538
HpyAV CCTTC 2 cut(s) 155, 230
HpyCH4III ACNGT 2 cut(s) 71, 259
HpyCH4V TGCA 4 cut(s) 335, 389, 538, 720
HpyF10VI GCNNNNNNNGC 2 cut(s) 298, 307
HpyF3I CTNAG 1 cut(s) 285
Hsp92II CATG 4 cut(s) 107, 335, 580, 704
Kzo9I GATC 4 cut(s) 4, 28, 164, 684
LpnPI CCDG 7 cut(s) 17, 612, 631, 691, 706, 723, 741
Lsp1109I GCAGC 1 cut(s) 347
LweI GCATC 3 cut(s) 301, 625, 707
MaeI CTAG 5 cut(s) 18, 222, 306, 375, 672
MalI GATC 4 cut(s) 6, 30, 166, 686
MboI GATC 4 cut(s) 4, 28, 164, 684
MboII GAAGA 9 cut(s) 86, 89, 101, 150, 262, 427, 484, 487, 502
MhlI GDGCHC 1 cut(s) 540
MluCI AATT 3 cut(s) 122, 243, 360
MlyI GAGTC 1 cut(s) 101
MmeI TCCRAC 2 cut(s) 90, 568
MnlI CCTC 6 cut(s) 233, 321, 606, 606, 664, 706
MseI TTAA 1 cut(s) 354
MwoI GCNNNNNNNGC 2 cut(s) 298, 307
NcoI CCATGG 1 cut(s) 103
NdeII GATC 4 cut(s) 4, 28, 164, 684
NlaIII CATG 4 cut(s) 107, 335, 580, 704
NlaIV GGNNCC 2 cut(s) 321, 726
NspI RCATGY 1 cut(s) 335
PceI AGGCCT 1 cut(s) 310
PfeI GAWTC 1 cut(s) 62
PkrI GCNGC 2 cut(s) 337, 384
PleI GAGTC 1 cut(s) 100
PpsI GAGTC 1 cut(s) 100
PspN4I GGNNCC 2 cut(s) 321, 726
PspPI GGNCC 2 cut(s) 320, 725
SaqAI TTAA 1 cut(s) 354
SatI GCNGC 2 cut(s) 336, 383
Sau3AI GATC 4 cut(s) 4, 28, 164, 684
Sau96I GGNCC 2 cut(s) 320, 725
SchI GAGTC 1 cut(s) 101
SduI GDGCHC 1 cut(s) 540
SfaNI GCATC 3 cut(s) 301, 625, 707
SinI GGWCC 1 cut(s) 320
Sse9I AATT 3 cut(s) 122, 243, 360
SseBI AGGCCT 1 cut(s) 310
SsiI CCGC 1 cut(s) 382
SspI AATATT 1 cut(s) 211
SspMI CTAG 5 cut(s) 18, 222, 306, 375, 672
StuI AGGCCT 1 cut(s) 310
StyI CCWWGG 4 cut(s) 103, 305, 514, 621
TaaI ACNGT 2 cut(s) 71, 259
TaqI TCGA 1 cut(s) 687
TasI AATT 3 cut(s) 122, 243, 360
TauI GCSGC 1 cut(s) 385
TfiI GAWTC 1 cut(s) 62
Tru1I TTAA 1 cut(s) 354
Tru9I TTAA 1 cut(s) 354
TscAI CASTG 3 cut(s) 76, 640, 735
TseI GCWGC 1 cut(s) 335
TspDTI ATGAA 3 cut(s) 290, 437, 536
TspRI CASTG 3 cut(s) 76, 640, 735
VneI GTGCAC 1 cut(s) 536
VpaK11BI GGWCC 1 cut(s) 320
XagI CCTNNNNNAGG 1 cut(s) 658
XapI RAATTY 1 cut(s) 360
XbaI TCTAGA 1 cut(s) 17
XceI RCATGY 1 cut(s) 335
XmaJI CCTAGG 1 cut(s) 305
XspI CTAG 5 cut(s) 18, 222, 306, 375, 672
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.