Rorug03G0020000

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0013695
Physical Location & Seq
Reverse (-)
1588918 .. 1591040
2123 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0020000.1

Sequence Viewer

Length: 1761 bp
ATGGCAATAGTCTCCACTGAGTTATTGTCCCATAGCTTCCACACTACCTCCCAACTCAAACCCACTTCCCATTCCCACCACCCCACTAGTTTCAGTTGCAGACCTTCTTCTTCTTCAATCTCAAATGGCAGAAACAACACCTCCAGAAACCTAACAAGGGTGTCTGTCTCTGCTGAACCAAAGCCAACACACTTGCAAAACCATGATTACAAAGAGACCCACTTGATCAAAGTCCTCAACAGGTCTTGCAAAGCAGGCCAGTACAATGAAGCCTTCTACTTTCTTGAGCTCATGGTCAACAAGGGCTACAAGCCTGATGTGATTCTCTGCACCAAGCTCATCAAAGGCTTCTTCAACTCGAGAAACATCCAAAAGGCCATTCGGGTTATGCAAATATTGGAACAGTATGGGGAGCCTGATTTGTTTTCATACAATGCTTTGATCAGTGGGTTCTGTAAGGCCAATAGGATTGAATCAGCAAACAAAGTTCTTGATAGAATGAAGAGTCAGGGGTTCAAGCCTGATGTTGTTACGTATAATATAATGATTGGGAGTCTCTGTAGTAGAGGGAAGCTTGGGTTAGCTAGGAAGGTTATGGATCGTTTAGTGAAAGACAATTGCAAGCCTACTGTGATTACATATACAATATTGATAGAAGCTACTATTCTTGATGGTGGGATTGATGAAGCTATGAAGCTTTTGGATGAGATGTTGTCAAGAGGGCTTAGACCTGATATGTATACTTACAATGCCATTGTTAGGGGAATGTGCAGGGAAGGAATGCTGGATAGAGCTTTCGAGTTTGTTAGGAGCTTCGATGCTAAAGGCTGTGCGCCGAATGTGATCTCATACAACATATTGTTGCGTGCGCTTTTGAACCGTGGGAAGTGGGAGGAAGGAGAGAATCTAGTGAGCAATATGTGTGCAAGAGGTTGCGAGCCTAATGTGGTGACTTATAGCATCTTGATTAGCACGCTGTGCCGCGATGGGAAAGTTGAGGATGCAGTGAATGTGTTGAAGATTATGAAGGAAAAGGGGTTAACTCCAGATGCTTATAGTTATGATCCATTGATTTCTACTTTCTGCAAAGAAGGGAGGTTAGATTTAGCAATAGAGTTTTTGGATTGTATGATATCTGATGGTTGCTTGCCAGATATTGTGAACTACAATACAATCTTGGCTGCTTTGTGCAAGAATGGGAGTGCTGATCAGGCTTTGGAAATCTTCGAGAACTTGGGTGAAGTCGGTTGTCCTCCAAATGTGAGTTCCTACAACACAATGTTCAGTGCATTGTGGAATTGTGGAGATAGAGTAAGAGCTTTGGGAATGGTATCAGACATGGTAAGCAAAGGAATTGAGCCTGATGAGATCACATACAATTCACTTATATCGTGTCTGTGCAGAGATGGGATGGTAAATGAGGCAATTGGGTTGTTGGTAGATATGGAAACTGGTGGTTTTCAGCCTACAGTTATCACTTACAATATTGTTCTTCTTGGGTTGAGCAAGACTCGGAGGATTATCGATGCCATTGAAGTGTTCACAGCAATGGTTGAAAAGGGTTGCCGGCCTAACGAAACTACTTACATTTTGCTGATTGAAGGAATTGGATTTGCAGGATGGCGAGCTGAAGCTATGGAGCTTGCAAAATCTGTTTATAGTTTAAGGGCTATTTCCGAAGATTCTTTCAAACGCTTGAACAGGACCTTTCCCATGCTTGATGTTTACAAAGAGCTTACTCTGTCTGAGATTAAGAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

586

Amino Acids

65.43

Weight (kDa)

7.03

Isoelectric Point (pI)

33.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_long PF17177 64 - 216 6.7e-06 Pentacotripeptide-repeat region of PRORP
PPR_3 PF13812 96 - 151 1.6e-07 Pentatricopeptide repeat domain
PPR_3 PF13812 138 - 185 1.8e-09 Pentatricopeptide repeat domain
PPR_1 PF12854 138 - 168 6.2e-11 PPR repeat
PPR_2 PF13041 139 - 187 8.4e-19 PPR repeat family
PPR PF01535 142 - 172 6.2e-08 PPR repeat
PPR_3 PF13812 162 - 220 5.3e-12 Pentatricopeptide repeat domain
PPR_1 PF12854 171 - 201 3.9e-09 PPR repeat
PPR_2 PF13041 174 - 220 1.7e-14 PPR repeat family
TPR_24 PF23276 190 - 298 3.6e-07 Fungal tetratrico peptide repeats
PPR_long PF17177 195 - 296 3.9e-06 Pentacotripeptide-repeat region of PRORP
PPR_1 PF12854 206 - 237 2.1e-07 PPR repeat
PPR_2 PF13041 225 - 258 1.7e-09 PPR repeat family
PPR_3 PF13812 232 - 291 4e-12 Pentatricopeptide repeat domain
PPR_1 PF12854 240 - 270 7.7e-11 PPR repeat
PPR_2 PF13041 248 - 292 9.4e-11 PPR repeat family
PPR_2 PF13041 279 - 328 5.9e-17 PPR repeat family
PPR_long PF17177 302 - 418 2.5e-11 Pentacotripeptide-repeat region of PRORP
PPR_3 PF13812 303 - 363 2.5e-10 Pentatricopeptide repeat domain
PPR_1 PF12854 310 - 343 7.2e-14 PPR repeat
PPR PF01535 317 - 347 4.8e-07 PPR repeat
PPR_2 PF13041 321 - 363 1.6e-10 PPR repeat family
PPR_1 PF12854 345 - 377 9.3e-10 PPR repeat
TPR_24 PF23276 351 - 443 2.3e-08 Fungal tetratrico peptide repeats
PPR_3 PF13812 377 - 430 9.2e-10 Pentatricopeptide repeat domain
PPR_1 PF12854 380 - 410 4.1e-08 PPR repeat
PPR_2 PF13041 384 - 431 2.2e-13 PPR repeat family
PPR_2 PF13041 419 - 468 1.5e-13 PPR repeat family
PPR_long PF17177 440 - 534 3.2e-07 Pentacotripeptide-repeat region of PRORP
PPR_3 PF13812 444 - 498 1.9e-08 Pentatricopeptide repeat domain
PPR_1 PF12854 450 - 483 2.2e-13 PPR repeat
PPR_2 PF13041 454 - 503 1.8e-14 PPR repeat family
PPR PF01535 457 - 486 2e-07 PPR repeat
PPR_3 PF13812 478 - 537 1.5e-07 Pentatricopeptide repeat domain
PPR_2 PF13041 489 - 535 2.6e-11 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013695)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g07660 FvH4_6g07660
malus_domestica MD12G1204500.v1.1
prunus_persica Prupe.6G311200_v2.0.a1 Prupe.6G311200_v2.0.a1 Prupe.6G311200_v2.0.a1
pyrus_communis pycom12g19190
rosa_chinensis RchiOBHm_Chr3g0457351
rosa_laevigata RLG00000025233
rosa_multiflora Rmu_sc0005442.1_g000009
rosa_roxburghii Rroxscaffold_6G00421960
rosa_rugosa Rorug03G0019900 Rorug03G0020000
rosa_samantha Rh3AG080200 Rh3BG082000 Rh3CG082300 Rh3DG083700
rosa_wichuraiana Rw3G006900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 740
AccII CGCG 1 cut(s) 984
AciI CCGC 1 cut(s) 982
AclWI GGATC 2 cut(s) 606, 1058
AcuI CTGAAG 1 cut(s) 1650
AdeI CACNNNGTG 1 cut(s) 978
AfaI GTAC 1 cut(s) 263
AfiI CCNNNNNNNGG 2 cut(s) 157, 759
AhlI ACTAGT 1 cut(s) 86
AjuI GAANNNNNNNTTGG 4 cut(s) 363, 395, 1249, 1281
Alw21I GWGCWC 1 cut(s) 291
Alw26I GTCTC 4 cut(s) 16, 172, 209, 560
AlwI GGATC 2 cut(s) 606, 1058
Ama87I CYCGRG 1 cut(s) 358
AoxI GGCC 4 cut(s) 256, 375, 459, 1568
ApeKI GCWGC 1 cut(s) 1181
AspLEI GCGC 2 cut(s) 835, 871
AspS9I GGNCC 1 cut(s) 1704
AsuHPI GGTGA 2 cut(s) 961, 1250
AvaI CYCGRG 1 cut(s) 358
AvaII GGWCC 1 cut(s) 1704
BanII GRGCYC 1 cut(s) 291
Bbv12I GWGCWC 1 cut(s) 291
BbvI GCAGC 1 cut(s) 1168
BccI CCATC 6 cut(s) 665, 980, 1133, 1400, 1405, 1614
BclI TGATCA 3 cut(s) 225, 441, 1207
BcoDI GTCTC 4 cut(s) 16, 172, 209, 560
BcuI ACTAGT 1 cut(s) 86
BfaI CTAG 4 cut(s) 87, 585, 908, 1759
BfmI CTRYAG 2 cut(s) 559, 1467
BisI GCNGC 2 cut(s) 982, 1182
BlsI GCNGC 2 cut(s) 983, 1183
Bme18I GGWCC 1 cut(s) 1704
BmeT110I CYCGRG 1 cut(s) 358
BmgT120I GGNCC 1 cut(s) 1704
BmiI GGNNCC 1 cut(s) 414
BmsI GCATC 5 cut(s) 808, 969, 991, 1039, 1516
BplI GAGNNNNNCTC 2 cut(s) 1495, 1527
BpmI CTGGAG 2 cut(s) 127, 1029
BpuEI CTTGAG 1 cut(s) 305
Bsa29I ATCGAT 1 cut(s) 1524
BsaAI YACGTR 1 cut(s) 534
BsaI GGTCTC 1 cut(s) 209
BsaJI CCNNGG 1 cut(s) 880
BsaXI ACNNNNNCTCC 8 cut(s) 32, 62, 125, 155, 544, 574, 1296, 1326
Bsc4I CCNNNNNNNGG 2 cut(s) 157, 759
Bse118I RCCGGY 1 cut(s) 1566
Bse1I ACTGG 2 cut(s) 259, 1456
Bse3DI GCAATG 1 cut(s) 1554
BseCI ATCGAT 1 cut(s) 1524
BseDI CCNNGG 1 cut(s) 880
BseGI GGATG 5 cut(s) 366, 709, 1006, 1416, 1625
BseLI CCNNNNNNNGG 2 cut(s) 157, 759
BseMI GCAATG 1 cut(s) 1554
BseMII CTCAG 2 cut(s) 9, 1737
BseNI ACTGG 2 cut(s) 259, 1456
BseXI GCAGC 1 cut(s) 1168
BsgI GTGCAG 3 cut(s) 313, 790, 1420
Bsh1236I CGCG 1 cut(s) 984
BshFI GGCC 4 cut(s) 258, 377, 461, 1570
BshVI ATCGAT 1 cut(s) 1524
BsiHKAI GWGCWC 1 cut(s) 291
BsiHKCI CYCGRG 1 cut(s) 358
BsiSI CCGG 1 cut(s) 1567
BslFI GGGAC 1 cut(s) 13
BslI CCNNNNNNNGG 2 cut(s) 157, 759
BsmAI GTCTC 4 cut(s) 16, 172, 209, 560
BsmFI GGGAC 1 cut(s) 13
BsmI GAATGC 1 cut(s) 786
BsnI GGCC 4 cut(s) 258, 377, 461, 1570
Bso31I GGTCTC 1 cut(s) 209
BsoBI CYCGRG 1 cut(s) 358
Bsp1286I GDGCHC 1 cut(s) 291
Bsp143I GATC 7 cut(s) 225, 441, 598, 843, 1063, 1207, 1368
BspACI CCGC 1 cut(s) 982
BspANI GGCC 4 cut(s) 258, 377, 461, 1570
BspCNI CTCAG 2 cut(s) 10, 1738
BspDI ATCGAT 1 cut(s) 1524
BspFNI CGCG 1 cut(s) 984
BspLI GGNNCC 1 cut(s) 414
BspPI GGATC 2 cut(s) 606, 1058
BspTNI GGTCTC 1 cut(s) 209
BsrDI GCAATG 1 cut(s) 1554
BsrFI RCCGGY 1 cut(s) 1566
BsrI ACTGG 2 cut(s) 259, 1456
BssAI RCCGGY 1 cut(s) 1566
BssECI CCNNGG 1 cut(s) 880
BssMI GATC 7 cut(s) 225, 441, 598, 843, 1063, 1207, 1368
BssNAI GTATAC 1 cut(s) 741
Bst1107I GTATAC 1 cut(s) 741
Bst4CI ACNGT 4 cut(s) 405, 631, 881, 1471
Bst6I CTCTTC 1 cut(s) 497
BstAPI GCANNNNNTGC 1 cut(s) 978
BstBAI YACGTR 1 cut(s) 534
BstC8I GCNNGC 9 cut(s) 256, 623, 867, 938, 974, 1148, 1568, 1626, 1644
BstDEI CTNAG 3 cut(s) 18, 725, 1746
BstDSI CCRYGG 1 cut(s) 880
BstF5I GGATG 5 cut(s) 366, 709, 1006, 1416, 1625
BstFNI CGCG 1 cut(s) 984
BstHHI GCGC 2 cut(s) 835, 871
BstKTI GATC 7 cut(s) 228, 444, 601, 846, 1066, 1210, 1371
BstMAI GTCTC 4 cut(s) 16, 172, 209, 560
BstMBI GATC 7 cut(s) 225, 441, 598, 843, 1063, 1207, 1368
BstMWI GCNNNNNNNGC 3 cut(s) 255, 978, 1211
BstSFI CTRYAG 2 cut(s) 559, 1467
BstSNI TACGTA 1 cut(s) 534
BstUI CGCG 1 cut(s) 984
BstV1I GCAGC 1 cut(s) 1168
BstZ17I GTATAC 1 cut(s) 741
Bsu15I ATCGAT 1 cut(s) 1524
BsuRI GGCC 4 cut(s) 258, 377, 461, 1570
BsuTUI ATCGAT 1 cut(s) 1524
BtgI CCRYGG 1 cut(s) 880
BtgZI GCGATG 1 cut(s) 999
BtsCI GGATG 5 cut(s) 366, 709, 1006, 1416, 1625
BtsI GCAGTG 1 cut(s) 1011
BtsIMutI CAGTG 4 cut(s) 15, 451, 1011, 1291
Cac8I GCNNGC 9 cut(s) 256, 623, 867, 938, 974, 1148, 1568, 1626, 1644
CfoI GCGC 2 cut(s) 835, 871
Cfr10I RCCGGY 1 cut(s) 1566
Cfr13I GGNCC 1 cut(s) 1704
ClaI ATCGAT 1 cut(s) 1524
Csp6I GTAC 1 cut(s) 262
CviAII CATG 4 cut(s) 203, 292, 1339, 1714
CviQI GTAC 1 cut(s) 262
DdeI CTNAG 3 cut(s) 18, 725, 1746
DpnI GATC 7 cut(s) 227, 443, 600, 845, 1065, 1209, 1370
DpnII GATC 7 cut(s) 225, 441, 598, 843, 1063, 1207, 1368
DraIII CACNNNGTG 1 cut(s) 978
Eam1104I CTCTTC 1 cut(s) 497
EarI CTCTTC 1 cut(s) 497
Ecl136II GAGCTC 1 cut(s) 289
Eco105I TACGTA 1 cut(s) 534
Eco24I GRGCYC 1 cut(s) 291
Eco31I GGTCTC 1 cut(s) 209
Eco32I GATATC 1 cut(s) 1134
Eco47I GGWCC 1 cut(s) 1704
Eco53kI GAGCTC 1 cut(s) 289
Eco57I CTGAAG 1 cut(s) 1650
Eco88I CYCGRG 1 cut(s) 358
EcoICRI GAGCTC 1 cut(s) 289
EcoO109I RGGNCCY 1 cut(s) 1704
EcoRV GATATC 1 cut(s) 1134
EcoT38I GRGCYC 1 cut(s) 291
FaeI CATG 4 cut(s) 206, 295, 1342, 1717
FaqI GGGAC 1 cut(s) 13
FatI CATG 4 cut(s) 202, 291, 1338, 1713
FbaI TGATCA 3 cut(s) 225, 441, 1207
FblI GTMKAC 1 cut(s) 740
Fnu4HI GCNGC 2 cut(s) 982, 1182
FokI GGATG 5 cut(s) 353, 716, 1013, 1423, 1632
FriOI GRGCYC 1 cut(s) 291
Fsp4HI GCNGC 2 cut(s) 982, 1182
FspBI CTAG 4 cut(s) 87, 585, 908, 1759
GlaI GCGC 2 cut(s) 834, 870
GluI GCNGC 2 cut(s) 982, 1182
GsuI CTGGAG 2 cut(s) 127, 1029
HaeIII GGCC 4 cut(s) 258, 377, 461, 1570
HapII CCGG 1 cut(s) 1567
HhaI GCGC 2 cut(s) 835, 871
Hin1II CATG 4 cut(s) 206, 295, 1342, 1717
Hin6I GCGC 2 cut(s) 833, 869
HinP1I GCGC 2 cut(s) 833, 869
HincII GTYRAC 2 cut(s) 298, 1041
HindII GTYRAC 2 cut(s) 298, 1041
HindIII AAGCTT 2 cut(s) 572, 695
HinfI GANTC 7 cut(s) 322, 473, 505, 553, 904, 1510, 1682
HpaI GTTAAC 1 cut(s) 1041
HpaII CCGG 1 cut(s) 1567
HphI GGTGA 2 cut(s) 961, 1250
Hpy166II GTNNAC 6 cut(s) 298, 741, 1041, 1162, 1542, 1726
Hpy188I TCNGA 5 cut(s) 1138, 1336, 1515, 1678, 1747
Hpy188III TCNNGA 9 cut(s) 144, 284, 360, 491, 668, 717, 964, 1046, 1228
Hpy8I GTNNAC 6 cut(s) 298, 741, 1041, 1162, 1542, 1726
HpyAV CCTTC 8 cut(s) 114, 283, 583, 770, 890, 1021, 1085, 1595
HpyCH4III ACNGT 4 cut(s) 405, 631, 881, 1471
HpyCH4IV ACGT 1 cut(s) 533
HpyF10VI GCNNNNNNNGC 3 cut(s) 255, 978, 1211
HpyF3I CTNAG 3 cut(s) 18, 725, 1746
HpySE526I ACGT 1 cut(s) 533
Hsp92II CATG 4 cut(s) 206, 295, 1342, 1717
HspAI GCGC 2 cut(s) 833, 869
KroI GCCGGC 1 cut(s) 1566
KroNI GCCGGC 1 cut(s) 1568
Ksp22I TGATCA 3 cut(s) 225, 441, 1207
KspAI GTTAAC 1 cut(s) 1041
Kzo9I GATC 7 cut(s) 225, 441, 598, 843, 1063, 1207, 1368
LmnI GCTCC 3 cut(s) 412, 810, 1639
Lsp1109I GCAGC 1 cut(s) 1168
LweI GCATC 5 cut(s) 808, 969, 991, 1039, 1516
MaeI CTAG 4 cut(s) 87, 585, 908, 1759
MaeII ACGT 1 cut(s) 533
MaeIII GTNAC 2 cut(s) 529, 949
MalI GATC 7 cut(s) 227, 443, 600, 845, 1065, 1209, 1370
MboI GATC 7 cut(s) 225, 441, 598, 843, 1063, 1207, 1368
MboII GAAGA 9 cut(s) 99, 102, 105, 343, 514, 1030, 1216, 1484, 1691
MfeI CAATTG 2 cut(s) 616, 1425
MhlI GDGCHC 1 cut(s) 291
MluCI AATT 6 cut(s) 616, 1297, 1353, 1378, 1425, 1605
MlyI GAGTC 3 cut(s) 514, 562, 1504
MroNI GCCGGC 1 cut(s) 1566
MseI TTAA 3 cut(s) 1040, 1664, 1752
MslI CAYNNNNRTG 2 cut(s) 1535, 1547
MspI CCGG 1 cut(s) 1567
MunI CAATTG 2 cut(s) 616, 1425
Mva1269I GAATGC 1 cut(s) 786
MvnI CGCG 1 cut(s) 984
MwoI GCNNNNNNNGC 3 cut(s) 255, 978, 1211
NaeI GCCGGC 1 cut(s) 1568
NdeII GATC 7 cut(s) 225, 441, 598, 843, 1063, 1207, 1368
NgoMIV GCCGGC 1 cut(s) 1566
NlaIII CATG 4 cut(s) 206, 295, 1342, 1717
NlaIV GGNNCC 1 cut(s) 414
NmuCI GTSAC 1 cut(s) 949
PaeR7I CTCGAG 1 cut(s) 358
PctI GAATGC 1 cut(s) 786
PdiI GCCGGC 1 cut(s) 1568
PfeI GAWTC 4 cut(s) 322, 473, 904, 1682
PkrI GCNGC 2 cut(s) 983, 1183
PleI GAGTC 3 cut(s) 513, 561, 1504
PpsI GAGTC 3 cut(s) 513, 561, 1504
Ppu21I YACGTR 1 cut(s) 534
PpuMI RGGWCCY 1 cut(s) 1704
Psp124BI GAGCTC 1 cut(s) 291
Psp5II RGGWCCY 1 cut(s) 1704
PspN4I GGNNCC 1 cut(s) 414
PspPI GGNCC 1 cut(s) 1704
PspPPI RGGWCCY 1 cut(s) 1704
RsaI GTAC 1 cut(s) 263
RsaNI GTAC 1 cut(s) 262
RseI CAYNNNNRTG 2 cut(s) 1535, 1547
SacI GAGCTC 1 cut(s) 291
SaqAI TTAA 3 cut(s) 1040, 1664, 1752
SatI GCNGC 2 cut(s) 982, 1182
Sau3AI GATC 7 cut(s) 225, 441, 598, 843, 1063, 1207, 1368
Sau96I GGNCC 1 cut(s) 1704
SchI GAGTC 3 cut(s) 514, 562, 1504
SduI GDGCHC 1 cut(s) 291
SfaNI GCATC 5 cut(s) 808, 969, 991, 1039, 1516
SfcI CTRYAG 2 cut(s) 559, 1467
Sfr274I CTCGAG 1 cut(s) 358
SinI GGWCC 1 cut(s) 1704
SlaI CTCGAG 1 cut(s) 358
SmiMI CAYNNNNRTG 2 cut(s) 1535, 1547
SmlI CTYRAG 2 cut(s) 284, 358
SmoI CTYRAG 2 cut(s) 284, 358
SnaBI TACGTA 1 cut(s) 534
SpeI ACTAGT 1 cut(s) 86
Sse9I AATT 6 cut(s) 616, 1297, 1353, 1378, 1425, 1605
SsiI CCGC 1 cut(s) 982
SspI AATATT 3 cut(s) 396, 648, 1486
SspMI CTAG 4 cut(s) 87, 585, 908, 1759
SstI GAGCTC 1 cut(s) 291
TaaI ACNGT 4 cut(s) 405, 631, 881, 1471
TaiI ACGT 1 cut(s) 536
TaqI TCGA 5 cut(s) 359, 798, 816, 1227, 1524
TasI AATT 6 cut(s) 616, 1297, 1353, 1378, 1425, 1605
TatI WGTACW 1 cut(s) 261
TauI GCSGC 1 cut(s) 984
TfiI GAWTC 4 cut(s) 322, 473, 904, 1682
Tru1I TTAA 3 cut(s) 1040, 1664, 1752
Tru9I TTAA 3 cut(s) 1040, 1664, 1752
TscAI CASTG 4 cut(s) 22, 451, 1011, 1291
TseFI GTSAC 1 cut(s) 949
TseI GCWGC 1 cut(s) 1181
Tsp45I GTSAC 1 cut(s) 949
TspDTI ATGAA 6 cut(s) 282, 417, 515, 699, 707, 1040
TspRI CASTG 4 cut(s) 22, 451, 1011, 1291
VpaK11BI GGWCC 1 cut(s) 1704
XhoI CTCGAG 1 cut(s) 358
XmiI GTMKAC 1 cut(s) 740
XspI CTAG 4 cut(s) 87, 585, 908, 1759
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.