MD12G1222100.v1.1

Basic 7S

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0016359
Physical Location & Seq
Reverse (-)
29895257 .. 29896606
1350 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1222100.v1.1.491

Sequence Viewer

Length: 1350 bp
ATGGCTTCTTTCCTTCCCAATTTGCTCCTACTCTTCTCTTGCTTTTCCCTTCTCATTTTCACGTCTGTGGCCACAGTAAAAACCCGACCCCAAATCCCCATCAAGCCAAATAAGCTTGTCCTCAAGGTGCAGAAAGATGGGGCAACCAATCTTCATGTGGCCCAAATTTACAAAAGAAACCCTCCAGTCCAATTCCCCTTTGTGATAGACCTAAATGGGAGGTTCTTATCAGTCAATTGTGAGAATCAGTACTTGTCATCGGGCTACAATGCTCCCGTTTGCCACTCAACACAGTGTGCTAGAGCCAATCCTAATAGCCAGATGACATGCCGCACATGCAGCTCGTCTAGAACCCGACTAGGGTGCCACTCCAACGCGTGTGGGCTCATGACCACAAACCCTGTGACGCGCCAAAGCGCCATGGGTGAGCTTGCTCAAGATGTCCTCTCAATCCCATCAACCCAAGGCTCGAGCCCTGGCCCAATGGTCCAAGTCCCTCAATTCCTCTTTGCATGTGCACCTTCAAATATTATGCAAAAAGGGCTACCCAAAAATGTTCAAGGTTTTGCTGGCCTAGGCCACTCCTCAATTTCCCTACCCTACCAACTAGCCTCCCACTTTGGCTTTCCAGCAAAATTTGCCGTGTGCCTCCCTTCTGCACCTGGCCAGAATGGCGTCATTTTCTTCGGGGAAGGACCCTATCTCATGAACCGGGGCATCGACGTGTCGCGCAAGTTGACCTACACCCCACTGACCATAGGCAGGCAAGGAGAGTACTACATAAACGTTCAATCCATCAAAATCAACAACAAGGTGGTTCCACTCAACACCTCTCTCTTGCCCTACTCACCAAAACGAGGGGTTGGTGGGACAATGATCAGCACCACAACTCCCTACACAATCCTCCAAACCTCTATCTTTAGGGCAGTCACTCAGCTCCTCATCAATCAGCTCCAAGGGGTACCCCAAGTGAAGCCCATGGCGCCATTCGGGGTGTGTTTCGATGCGAAGAAATTTGCCAGCAACAAGGCAGCGCTTACGGTTTCTAGCATAGACCTTGTTCTAGACAACAACCGGAACATCTGGAGGCTGTATGGTCATAACGTTATCGTTCAGCCAAGTCCCAACGTGGCGTGTCTAGGGTTTGTGGACGGTGGGTTGAGGCCTCAGGCTTCGATCGTGATCGGAGCACTCCAGTTGGAGGATAATCTGCTGCAGTTTGATTTGACGAACTCAAGGTTGGGATTTAGCTCTTCGTTGTTGTCCCGCAGGACTAGTTGTTCCAACTTCAACTTTGGCAAATCCTCCACTGGTGCTGAAGAATCTACTGAATCCACCGATGAGCCATAA

Protein Analysis

450

Amino Acids

48.61

Weight (kDa)

9.45

Isoelectric Point (pI)

46.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TAXi_N PF14543 54 - 230 1.5e-34 Xylanase inhibitor N-terminal
TAXi_C PF14541 258 - 417 1.8e-51 Xylanase inhibitor C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016359)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g05510
malus_domestica MD04G1208100.v1.1 MD12G1222100.v1.1
prunus_persica Prupe.6G329000_v2.0.a1
pyrus_communis pycom12g20830
rosa_chinensis RchiOBHm_Chr3g0454271
rosa_laevigata RLG00000025468
rosa_roxburghii Rroxscaffold_165G00437090 Rroxscaffold_6G00425250
rosa_rugosa Rorug02G0653800
rosa_samantha Rh3AG057600 Rh3BG059300 Rh3CG058500 Rh3DG059800
rosa_wichuraiana Rw3G004420

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 961
AccB1I GGYRCC 3 cut(s) 363, 961, 982
AccII CGCG 3 cut(s) 377, 409, 730
AciI CCGC 2 cut(s) 331, 1267
AclI AACGTT 2 cut(s) 786, 1104
AcoI YGGCCR 2 cut(s) 69, 664
AcsI RAATTY 3 cut(s) 165, 635, 1013
AcuI CTGAAG 1 cut(s) 1338
AcyI GRCGYC 2 cut(s) 675, 983
AdeI CACNNNGTG 1 cut(s) 296
AfaI GTAC 3 cut(s) 251, 776, 963
AfeI AGCGCT 1 cut(s) 1035
AfiI CCNNNNNNNGG 4 cut(s) 360, 762, 857, 1201
AflIII ACRYGT 2 cut(s) 375, 723
AgsI TTSAA 4 cut(s) 525, 560, 791, 1291
AhlI ACTAGT 1 cut(s) 1274
AjiI CACGTC 2 cut(s) 63, 724
AjnI CCWGG 2 cut(s) 475, 661
AjuI GAANNNNNNNTTGG 2 cut(s) 1223, 1255
AleI CACNNNNGTG 1 cut(s) 65
AloI GAACNNNNNNTCC 2 cut(s) 1264, 1296
AluBI AGCT 6 cut(s) 115, 342, 430, 937, 952, 1251
AluI AGCT 6 cut(s) 115, 342, 430, 937, 952, 1251
Alw21I GWGCWC 2 cut(s) 520, 1192
Alw44I GTGCAC 1 cut(s) 516
Ama87I CYCGRG 1 cut(s) 469
Aor51HI AGCGCT 1 cut(s) 1035
AoxI GGCC 7 cut(s) 69, 159, 478, 571, 577, 664, 1163
ApaLI GTGCAC 1 cut(s) 516
ApeKI GCWGC 3 cut(s) 339, 1031, 1213
ApoI RAATTY 3 cut(s) 165, 635, 1013
Asp718I GGTACC 1 cut(s) 961
AspA2I CCTAGG 1 cut(s) 574
AspLEI GCGC 5 cut(s) 411, 419, 732, 985, 1036
AspS9I GGNCC 4 cut(s) 160, 479, 487, 695
AsuC2I CCSGG 1 cut(s) 713
AsuHPI GGTGA 2 cut(s) 437, 840
AvaI CYCGRG 1 cut(s) 469
AvaII GGWCC 2 cut(s) 487, 695
AvrII CCTAGG 1 cut(s) 574
AxyI CCTNAGG 1 cut(s) 1167
BaeGI GKGCMC 1 cut(s) 520
BalI TGGCCA 2 cut(s) 71, 666
BanI GGYRCC 3 cut(s) 363, 961, 982
BanII GRGCYC 2 cut(s) 387, 476
Bbv12I GWGCWC 2 cut(s) 520, 1192
BbvI GCAGC 3 cut(s) 351, 1043, 1200
BccI CCATC 4 cut(s) 107, 131, 463, 803
BceAI ACGGC 1 cut(s) 626
BcgI CGANNNNNNTGC 2 cut(s) 345, 379
BciT130I CCWGG 2 cut(s) 477, 663
BclI TGATCA 1 cut(s) 876
BcnI CCSGG 1 cut(s) 713
BcuI ACTAGT 1 cut(s) 1274
BfaI CTAG 9 cut(s) 300, 348, 359, 575, 608, 1047, 1064, 1139, 1275
BfmI CTRYAG 1 cut(s) 1214
BfoI RGCGCY 3 cut(s) 420, 986, 1037
BglI GCCNNNNNGGC 1 cut(s) 672
BisI GCNGC 4 cut(s) 331, 340, 1032, 1214
BlnI CCTAGG 1 cut(s) 574
BlsI GCNGC 4 cut(s) 332, 341, 1033, 1215
BmcAI AGTACT 2 cut(s) 251, 776
Bme1390I CCNGG 3 cut(s) 477, 663, 713
Bme18I GGWCC 2 cut(s) 487, 695
BmeT110I CYCGRG 1 cut(s) 469
BmgBI CACGTC 2 cut(s) 63, 724
BmgT120I GGNCC 4 cut(s) 160, 479, 487, 695
BmiI GGNNCC 5 cut(s) 365, 697, 819, 963, 984
BmrFI CCNGG 3 cut(s) 477, 663, 713
BmsI GCATC 2 cut(s) 726, 994
BpmI CTGGAG 3 cut(s) 168, 1105, 1178
BpuEI CTTGAG 3 cut(s) 107, 420, 1219
BpuMI CCSGG 1 cut(s) 713
BsaBI GATNNNNATC 1 cut(s) 1181
BsaHI GRCGYC 2 cut(s) 675, 983
BsaJI CCNNGG 7 cut(s) 420, 463, 475, 574, 712, 955, 978
BsaWI WCCGGW 1 cut(s) 1074
BsaXI ACNNNNNCTCC 2 cut(s) 874, 904
Bsc4I CCNNNNNNNGG 4 cut(s) 360, 762, 857, 1201
Bse1I ACTGG 3 cut(s) 185, 1195, 1315
Bse21I CCTNAGG 1 cut(s) 1167
Bse8I GATNNNNATC 1 cut(s) 1181
BseBI CCWGG 2 cut(s) 477, 663
BseDI CCNNGG 7 cut(s) 420, 463, 475, 574, 712, 955, 978
BseJI GATNNNNATC 1 cut(s) 1181
BseLI CCNNNNNNNGG 4 cut(s) 360, 762, 857, 1201
BseMII CTCAG 2 cut(s) 947, 1181
BseNI ACTGG 3 cut(s) 185, 1195, 1315
BseRI GAGGAG 2 cut(s) 574, 929
BseSI GKGCMC 1 cut(s) 520
BseXI GCAGC 3 cut(s) 351, 1043, 1200
BsgI GTGCAG 2 cut(s) 149, 642
Bsh1236I CGCG 3 cut(s) 377, 409, 730
Bsh1285I CGRYCG 1 cut(s) 1179
BshFI GGCC 7 cut(s) 71, 161, 480, 573, 579, 666, 1165
BshNI GGYRCC 3 cut(s) 363, 961, 982
BsiEI CGRYCG 1 cut(s) 1179
BsiHKAI GWGCWC 2 cut(s) 520, 1192
BsiHKCI CYCGRG 1 cut(s) 469
BsiSI CCGG 2 cut(s) 712, 1075
BslFI GGGAC 4 cut(s) 479, 883, 1107, 1249
BslI CCNNNNNNNGG 4 cut(s) 360, 762, 857, 1201
BsmFI GGGAC 4 cut(s) 479, 883, 1107, 1249
BsnI GGCC 7 cut(s) 71, 161, 480, 573, 579, 666, 1165
BsoBI CYCGRG 1 cut(s) 469
Bsp1286I GDGCHC 4 cut(s) 387, 476, 520, 1192
Bsp143I GATC 3 cut(s) 876, 1176, 1182
Bsp19I CCATGG 2 cut(s) 420, 978
BspACI CCGC 2 cut(s) 331, 1267
BspANI GGCC 7 cut(s) 71, 161, 480, 573, 579, 666, 1165
BspCNI CTCAG 2 cut(s) 946, 1180
BspFNI CGCG 3 cut(s) 377, 409, 730
BspHI TCATGA 2 cut(s) 387, 705
BspLI GGNNCC 5 cut(s) 365, 697, 819, 963, 984
BspMAI CTGCAG 1 cut(s) 1218
BspQI GCTCTTC 1 cut(s) 1258
BspT107I GGYRCC 3 cut(s) 363, 961, 982
BsrI ACTGG 3 cut(s) 185, 1195, 1315
BssECI CCNNGG 7 cut(s) 420, 463, 475, 574, 712, 955, 978
BssMI GATC 3 cut(s) 876, 1176, 1182
BssNI GRCGYC 2 cut(s) 675, 983
BssT1I CCWWGG 5 cut(s) 420, 463, 574, 955, 978
Bst2UI CCWGG 2 cut(s) 477, 663
Bst4CI ACNGT 4 cut(s) 76, 294, 1042, 1154
Bst6I CTCTTC 2 cut(s) 38, 1258
BstACI GRCGYC 2 cut(s) 675, 983
BstAPI GCANNNNNTGC 1 cut(s) 638
BstC8I GCNNGC 4 cut(s) 432, 571, 764, 1021
BstDEI CTNAG 2 cut(s) 933, 1167
BstDSI CCRYGG 2 cut(s) 420, 978
BstFNI CGCG 3 cut(s) 377, 409, 730
BstH2I RGCGCY 3 cut(s) 420, 986, 1037
BstHHI GCGC 5 cut(s) 411, 419, 732, 985, 1036
BstKTI GATC 3 cut(s) 879, 1179, 1185
BstMBI GATC 3 cut(s) 876, 1176, 1182
BstMCI CGRYCG 1 cut(s) 1179
BstMWI GCNNNNNNNGC 7 cut(s) 112, 336, 339, 541, 638, 672, 982
BstNI CCWGG 2 cut(s) 477, 663
BstNSI RCATGY 3 cut(s) 330, 339, 516
BstSCI CCNGG 3 cut(s) 475, 661, 711
BstSFI CTRYAG 1 cut(s) 1214
BstSLI GKGCMC 1 cut(s) 520
BstUI CGCG 3 cut(s) 377, 409, 730
BstV1I GCAGC 3 cut(s) 351, 1043, 1200
Bsu36I CCTNAGG 1 cut(s) 1167
BsuRI GGCC 7 cut(s) 71, 161, 480, 573, 579, 666, 1165
BtgI CCRYGG 2 cut(s) 420, 978
BtrI CACGTC 2 cut(s) 63, 724
BtsIMutI CAGTG 3 cut(s) 299, 749, 1308
Cac8I GCNNGC 4 cut(s) 432, 571, 764, 1021
CciI TCATGA 2 cut(s) 387, 705
CfoI GCGC 5 cut(s) 411, 419, 732, 985, 1036
Cfr13I GGNCC 4 cut(s) 160, 479, 487, 695
CseI GACGC 2 cut(s) 415, 664
Csp6I GTAC 3 cut(s) 250, 775, 962
CspCI CAANNNNNGTGG 2 cut(s) 361, 396
CviAII CATG 8 cut(s) 155, 327, 336, 388, 421, 513, 706, 979
CviQI GTAC 3 cut(s) 250, 775, 962
DdeI CTNAG 2 cut(s) 933, 1167
DinI GGCGCC 1 cut(s) 984
DpnI GATC 3 cut(s) 878, 1178, 1184
DpnII GATC 3 cut(s) 876, 1176, 1182
DraIII CACNNNGTG 1 cut(s) 296
EaeI YGGCCR 2 cut(s) 69, 664
Eam1104I CTCTTC 2 cut(s) 38, 1258
EarI CTCTTC 2 cut(s) 38, 1258
Eco130I CCWWGG 5 cut(s) 420, 463, 574, 955, 978
Eco147I AGGCCT 1 cut(s) 1165
Eco24I GRGCYC 2 cut(s) 387, 476
Eco47I GGWCC 2 cut(s) 487, 695
Eco47III AGCGCT 1 cut(s) 1035
Eco57I CTGAAG 1 cut(s) 1338
Eco81I CCTNAGG 1 cut(s) 1167
Eco88I CYCGRG 1 cut(s) 469
EcoO109I RGGNCCY 1 cut(s) 695
EcoRII CCWGG 2 cut(s) 475, 661
EcoT14I CCWWGG 5 cut(s) 420, 463, 574, 955, 978
EcoT38I GRGCYC 2 cut(s) 387, 476
EgeI GGCGCC 1 cut(s) 984
EheI GGCGCC 1 cut(s) 984
ErhI CCWWGG 5 cut(s) 420, 463, 574, 955, 978
FaeI CATG 8 cut(s) 158, 330, 339, 391, 424, 516, 709, 982
FaqI GGGAC 4 cut(s) 479, 883, 1107, 1249
FatI CATG 8 cut(s) 154, 326, 335, 387, 420, 512, 705, 978
FauI CCCGC 1 cut(s) 1274
FbaI TGATCA 1 cut(s) 876
Fnu4HI GCNGC 4 cut(s) 331, 340, 1032, 1214
FriOI GRGCYC 2 cut(s) 387, 476
Fsp4HI GCNGC 4 cut(s) 331, 340, 1032, 1214
FspBI CTAG 9 cut(s) 300, 348, 359, 575, 608, 1047, 1064, 1139, 1275
GlaI GCGC 5 cut(s) 410, 418, 731, 984, 1035
GluI GCNGC 4 cut(s) 331, 340, 1032, 1214
GsuI CTGGAG 3 cut(s) 168, 1105, 1178
HaeII RGCGCY 3 cut(s) 420, 986, 1037
HaeIII GGCC 7 cut(s) 71, 161, 480, 573, 579, 666, 1165
HapII CCGG 2 cut(s) 712, 1075
HgaI GACGC 2 cut(s) 415, 664
HhaI GCGC 5 cut(s) 411, 419, 732, 985, 1036
Hin1I GRCGYC 2 cut(s) 675, 983
Hin1II CATG 8 cut(s) 158, 330, 339, 391, 424, 516, 709, 982
Hin6I GCGC 5 cut(s) 409, 417, 730, 983, 1034
HinP1I GCGC 5 cut(s) 409, 417, 730, 983, 1034
HincII GTYRAC 1 cut(s) 738
HindII GTYRAC 1 cut(s) 738
HindIII AAGCTT 1 cut(s) 113
HinfI GANTC 3 cut(s) 244, 1322, 1331
HpaII CCGG 2 cut(s) 712, 1075
HphI GGTGA 2 cut(s) 437, 840
Hpy166II GTNNAC 3 cut(s) 518, 738, 1150
Hpy188I TCNGA 1 cut(s) 1187
Hpy188III TCNNGA 7 cut(s) 348, 388, 437, 706, 1064, 1084, 1180
Hpy8I GTNNAC 3 cut(s) 518, 738, 1150
Hpy99I CGWCG 1 cut(s) 725
HpyAV CCTTC 5 cut(s) 23, 59, 531, 663, 686
HpyCH4III ACNGT 4 cut(s) 76, 294, 1042, 1154
HpyCH4IV ACGT 5 cut(s) 62, 723, 786, 1104, 1128
HpyCH4V TGCA 7 cut(s) 130, 339, 512, 518, 535, 659, 1216
HpyF10VI GCNNNNNNNGC 7 cut(s) 112, 336, 339, 541, 638, 672, 982
HpyF3I CTNAG 2 cut(s) 933, 1167
HpySE526I ACGT 5 cut(s) 62, 723, 786, 1104, 1128
Hsp92I GRCGYC 2 cut(s) 675, 983
Hsp92II CATG 8 cut(s) 158, 330, 339, 391, 424, 516, 709, 982
HspAI GCGC 5 cut(s) 409, 417, 730, 983, 1034
KasI GGCGCC 1 cut(s) 982
KpnI GGTACC 1 cut(s) 965
Ksp22I TGATCA 1 cut(s) 876
Kzo9I GATC 3 cut(s) 876, 1176, 1182
LguI GCTCTTC 1 cut(s) 1258
LmnI GCTCC 5 cut(s) 30, 277, 942, 957, 1187
Lsp1109I GCAGC 3 cut(s) 351, 1043, 1200
LweI GCATC 2 cut(s) 726, 994
MaeI CTAG 9 cut(s) 300, 348, 359, 575, 608, 1047, 1064, 1139, 1275
MaeII ACGT 5 cut(s) 62, 723, 786, 1104, 1128
MaeIII GTNAC 2 cut(s) 403, 928
MalI GATC 3 cut(s) 878, 1178, 1184
MboI GATC 3 cut(s) 876, 1176, 1182
MboII GAAGA 6 cut(s) 25, 143, 676, 1021, 1245, 1331
MfeI CAATTG 1 cut(s) 235
MhlI GDGCHC 4 cut(s) 387, 476, 520, 1192
MlsI TGGCCA 2 cut(s) 71, 666
MluCI AATT 8 cut(s) 19, 165, 191, 235, 500, 588, 635, 1013
MluI ACGCGT 1 cut(s) 375
MluNI TGGCCA 2 cut(s) 71, 666
Mly113I GGCGCC 1 cut(s) 983
MmeI TCCRAC 3 cut(s) 396, 1179, 1308
Mox20I TGGCCA 2 cut(s) 71, 666
MscI TGGCCA 2 cut(s) 71, 666
MslI CAYNNNNRTG 2 cut(s) 65, 722
Msp20I TGGCCA 2 cut(s) 71, 666
MspI CCGG 2 cut(s) 712, 1075
MspR9I CCNGG 3 cut(s) 477, 663, 713
MunI CAATTG 1 cut(s) 235
MvaI CCWGG 2 cut(s) 477, 663
MvnI CGCG 3 cut(s) 377, 409, 730
MwoI GCNNNNNNNGC 7 cut(s) 112, 336, 339, 541, 638, 672, 982
NarI GGCGCC 1 cut(s) 983
NciI CCSGG 1 cut(s) 713
NcoI CCATGG 2 cut(s) 420, 978
NdeII GATC 3 cut(s) 876, 1176, 1182
NlaIII CATG 8 cut(s) 158, 330, 339, 391, 424, 516, 709, 982
NlaIV GGNNCC 5 cut(s) 365, 697, 819, 963, 984
NmuCI GTSAC 2 cut(s) 403, 928
NspI RCATGY 3 cut(s) 330, 339, 516
OliI CACNNNNGTG 1 cut(s) 65
PaeR7I CTCGAG 1 cut(s) 469
PagI TCATGA 2 cut(s) 387, 705
PceI AGGCCT 1 cut(s) 1165
PciSI GCTCTTC 1 cut(s) 1258
PfeI GAWTC 3 cut(s) 244, 1322, 1331
PkrI GCNGC 4 cut(s) 332, 341, 1033, 1215
Ple19I CGATCG 1 cut(s) 1179
PluTI GGCGCC 1 cut(s) 986
PpuMI RGGWCCY 1 cut(s) 695
Psp1406I AACGTT 2 cut(s) 786, 1104
Psp5II RGGWCCY 1 cut(s) 695
Psp6I CCWGG 2 cut(s) 475, 661
PspGI CCWGG 2 cut(s) 475, 661
PspN4I GGNNCC 5 cut(s) 365, 697, 819, 963, 984
PspPI GGNCC 4 cut(s) 160, 479, 487, 695
PspPPI RGGWCCY 1 cut(s) 695
PspXI VCTCGAGB 1 cut(s) 469
PstI CTGCAG 1 cut(s) 1218
PvuI CGATCG 1 cut(s) 1179
RsaI GTAC 3 cut(s) 251, 776, 963
RsaNI GTAC 3 cut(s) 250, 775, 962
RseI CAYNNNNRTG 2 cut(s) 65, 722
SapI GCTCTTC 1 cut(s) 1258
SatI GCNGC 4 cut(s) 331, 340, 1032, 1214
Sau3AI GATC 3 cut(s) 876, 1176, 1182
Sau96I GGNCC 4 cut(s) 160, 479, 487, 695
ScaI AGTACT 2 cut(s) 251, 776
ScrFI CCNGG 3 cut(s) 477, 663, 713
SduI GDGCHC 4 cut(s) 387, 476, 520, 1192
SfaNI GCATC 2 cut(s) 726, 994
SfcI CTRYAG 1 cut(s) 1214
SfoI GGCGCC 1 cut(s) 984
Sfr274I CTCGAG 1 cut(s) 469
SinI GGWCC 2 cut(s) 487, 695
SlaI CTCGAG 1 cut(s) 469
SmiMI CAYNNNNRTG 2 cut(s) 65, 722
SmlI CTYRAG 4 cut(s) 122, 435, 469, 1234
SmoI CTYRAG 4 cut(s) 122, 435, 469, 1234
SpeI ACTAGT 1 cut(s) 1274
Sse9I AATT 8 cut(s) 19, 165, 191, 235, 500, 588, 635, 1013
SseBI AGGCCT 1 cut(s) 1165
SsiI CCGC 2 cut(s) 331, 1267
SspDI GGCGCC 1 cut(s) 982
SspI AATATT 1 cut(s) 529
SspMI CTAG 9 cut(s) 300, 348, 359, 575, 608, 1047, 1064, 1139, 1275
StuI AGGCCT 1 cut(s) 1165
StyD4I CCNGG 3 cut(s) 475, 661, 711
StyI CCWWGG 5 cut(s) 420, 463, 574, 955, 978
TaaI ACNGT 4 cut(s) 76, 294, 1042, 1154
TaiI ACGT 5 cut(s) 65, 726, 789, 1107, 1131
TaqI TCGA 4 cut(s) 470, 720, 1002, 1175
TasI AATT 8 cut(s) 19, 165, 191, 235, 500, 588, 635, 1013
TatI WGTACW 2 cut(s) 249, 774
TauI GCSGC 1 cut(s) 333
TfiI GAWTC 3 cut(s) 244, 1322, 1331
TscAI CASTG 3 cut(s) 299, 756, 1315
TseFI GTSAC 2 cut(s) 403, 928
TseI GCWGC 3 cut(s) 339, 1031, 1213
Tsp45I GTSAC 2 cut(s) 403, 928
TspDTI ATGAA 2 cut(s) 143, 722
TspRI CASTG 3 cut(s) 299, 756, 1315
VneI GTGCAC 1 cut(s) 516
VpaK11BI GGWCC 2 cut(s) 487, 695
XapI RAATTY 3 cut(s) 165, 635, 1013
XbaI TCTAGA 2 cut(s) 347, 1063
XceI RCATGY 3 cut(s) 330, 339, 516
XcmI CCANNNNNNNNNTGG 1 cut(s) 154
XhoI CTCGAG 1 cut(s) 469
XmaJI CCTAGG 1 cut(s) 574
XspI CTAG 9 cut(s) 300, 348, 359, 575, 608, 1047, 1064, 1139, 1275
ZrmI AGTACT 2 cut(s) 251, 776
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.