Prupe.6G329000_v2.0.a1

Basic 7S

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp06
OG0016359
Physical Location & Seq
Reverse (-)
28782867 .. 28784355
1489 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.6G329000.1

Sequence Viewer

Length: 1296 bp
ATGGCTTCTTTCCTTCATAATTTCCTGCTCTTCTTCTGTTCTTTATCTCTTATCATTCTCACATCTTCAGCAACCAAATCCCAAACCCACGTCCCCATCAGACCAAACAAGCTTGTCCTCAAAGTCCAGAAAGATAGGGCAACCAATCTCCATGTGGCCCAAATTCACAAAAGAACCCCTCTAGTCCAGTTCCCCTTTGTCATAGACCTAACTGGGAGGTTCTTATCAGTCAATTGTGAGAACCAATACACGTCATCAACTTACAAAGCCCCTGTTTGCCACTCAAGCCAGTGTGCTAGGGCCAACAGCCACATGTGCCGCACGTGCAGCTCTTTGAAAACCAGACCAGGCTGCCATACCAACGCTTGTGGGCTCTTGACCACGAACCCTGTGACCCAACAAAGTGCCCAGGGTGAGCTCGCAGAGGATGTCCTCAAAATCCCGTCAACCCAAGGCTCAAGCCCTGGCCCAATGGTCACACACCCCCATTTCCTCTTTGCTTGTGCACCTTCCAATATCTTGCAAAAGGGGTTACCCAAAAATGTTCAAGGTGTTGCTGGTCTAGGCCACTCCCCAATTTCCCTACCATACCAACTAGCTTCCCACTTTGGCTTCCCACCAAAATTTGCACTGTGCCTCACTTCCTCACCTGGCAAAAATGGCGCCGTTTTCTTCGGTGCAGGACCCTATTTCATGAAGCCGGGCATCGATGTGTCACGCCAGTTGACCTATGCCCCATTCACCGTAGGCCAGCAAGGAGAGTACTACATCAATATTCTATCAATTAAAATCAACAACGCCATGTTGCCTTCGATCCCAAAGGGTGGGTTTGGTGGGGCAATGATCAGCACCACAACTCCCTACACAACTCTCCAAACCCCAATTTTCAGGGCACTCAATCAGCTCTTCATGAACCAGCTTCGAGGGGTGCCTCATGTGAAACCCGTGGCACCATTTGGGGCATGTTTTGACGCAAACAGAATCCCTACCAGCAAGATGGGGCCTACGGTTCCCAGCATAGACCTTGTTCTGGACAATAAGAAAAACATCATGTGGAGAATTTTTGGTGCAAACGCTATGATTCAGCCACGTCCTGGCGTGATGTGTTTGGCGTTTGTGGACGGTGGGATGAGGCCTAAGGCTCCAATTGTGATTGGGACCCAGCAGTTGGAGGATAATCTGCTGCAGTTTGATTTGATGAACTCAAGGCTGGGATTTAGCTCTTCCTTGTTGTTCCGCAGGACCAACTGTGCCAACTTCAACTTTGGCACCTCCTCCACCAACACTGATCCGTAG

Protein Analysis

432

Amino Acids

46.85

Weight (kDa)

9.69

Isoelectric Point (pI)

42.62

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016359)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g05510
malus_domestica MD04G1208100.v1.1 MD12G1222100.v1.1
prunus_persica Prupe.6G329000_v2.0.a1
pyrus_communis pycom12g20830
rosa_chinensis RchiOBHm_Chr3g0454271
rosa_laevigata RLG00000025468
rosa_roxburghii Rroxscaffold_165G00437090 Rroxscaffold_6G00425250
rosa_rugosa Rorug02G0653800
rosa_samantha Rh3AG057600 Rh3BG059300 Rh3CG058500 Rh3DG059800
rosa_wichuraiana Rw3G004420

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 4 cut(s) 662, 928, 949, 1268
AccB7I CCANNNNNTGG 3 cut(s) 824, 1094, 1168
AciI CCGC 2 cut(s) 319, 1237
AclWI GGATC 2 cut(s) 808, 1283
AcsI RAATTY 3 cut(s) 162, 623, 1059
AcuI CTGAAG 1 cut(s) 51
AcvI CACGTG 1 cut(s) 324
AcyI GRCGYC 1 cut(s) 663
AfaI GTAC 1 cut(s) 764
AfiI CCNNNNNNNGG 4 cut(s) 824, 1030, 1094, 1168
AflIII ACRYGT 2 cut(s) 249, 312
AgsI TTSAA 3 cut(s) 337, 548, 1261
AjiI CACGTC 3 cut(s) 91, 252, 1091
AjnI CCWGG 5 cut(s) 346, 408, 463, 649, 1093
AluBI AGCT 7 cut(s) 112, 330, 418, 599, 904, 919, 1221
AluI AGCT 7 cut(s) 112, 330, 418, 599, 904, 919, 1221
Alw21I GWGCWC 2 cut(s) 420, 508
Alw44I GTGCAC 1 cut(s) 504
AlwI GGATC 2 cut(s) 808, 1283
AoxI GGCC 7 cut(s) 156, 300, 466, 565, 748, 1001, 1133
ApaLI GTGCAC 1 cut(s) 504
ApeKI GCWGC 3 cut(s) 327, 351, 1183
ApoI RAATTY 3 cut(s) 162, 623, 1059
AspLEI GCGC 1 cut(s) 665
AspS9I GGNCC 7 cut(s) 157, 300, 467, 683, 1001, 1158, 1242
AsuC2I CCSGG 1 cut(s) 702
AsuHPI GGTGA 3 cut(s) 425, 639, 733
AvaII GGWCC 3 cut(s) 683, 1158, 1242
AxyI CCTNAGG 1 cut(s) 1137
BaeGI GKGCMC 3 cut(s) 409, 508, 895
BanI GGYRCC 4 cut(s) 662, 928, 949, 1268
BanII GRGCYC 2 cut(s) 375, 420
BbrPI CACGTG 1 cut(s) 324
Bbv12I GWGCWC 2 cut(s) 420, 508
BbvI GCAGC 3 cut(s) 338, 339, 1170
BccI CCATC 2 cut(s) 104, 991
BceAI ACGGC 1 cut(s) 650
BciT130I CCWGG 5 cut(s) 348, 410, 465, 651, 1095
BclI TGATCA 1 cut(s) 843
BcnI CCSGG 1 cut(s) 702
BfaI CTAG 4 cut(s) 182, 297, 563, 596
BfmI CTRYAG 1 cut(s) 1184
BfoI RGCGCY 1 cut(s) 666
BisI GCNGC 4 cut(s) 319, 328, 352, 1184
BlsI GCNGC 4 cut(s) 320, 329, 353, 1185
BmcAI AGTACT 1 cut(s) 764
Bme1390I CCNGG 6 cut(s) 348, 410, 465, 651, 702, 1095
Bme18I GGWCC 3 cut(s) 683, 1158, 1242
BmgBI CACGTC 3 cut(s) 91, 252, 1091
BmgT120I GGNCC 7 cut(s) 157, 300, 467, 683, 1001, 1158, 1242
BmrFI CCNGG 6 cut(s) 348, 410, 465, 651, 702, 1095
BmrI ACTGGG 1 cut(s) 222
BmsI GCATC 1 cut(s) 714
BmuI ACTGGG 1 cut(s) 222
BpuEI CTTGAG 3 cut(s) 268, 442, 1189
BpuMI CCSGG 1 cut(s) 702
Bsa29I ATCGAT 1 cut(s) 708
BsaAI YACGTR 1 cut(s) 324
BsaHI GRCGYC 1 cut(s) 663
BsaJI CCNNGG 5 cut(s) 408, 409, 451, 463, 945
BsaXI ACNNNNNCTCC 2 cut(s) 841, 871
Bsc4I CCNNNNNNNGG 4 cut(s) 824, 1030, 1094, 1168
Bse1I ACTGG 4 cut(s) 187, 217, 289, 721
Bse21I CCTNAGG 1 cut(s) 1137
Bse3DI GCAATG 1 cut(s) 846
BseBI CCWGG 5 cut(s) 348, 410, 465, 651, 1095
BseCI ATCGAT 1 cut(s) 708
BseDI CCNNGG 5 cut(s) 408, 409, 451, 463, 945
BseGI GGATG 2 cut(s) 433, 1134
BseLI CCNNNNNNNGG 4 cut(s) 824, 1030, 1094, 1168
BseMI GCAATG 1 cut(s) 846
BseNI ACTGG 4 cut(s) 187, 217, 289, 721
BseRI GAGGAG 1 cut(s) 1264
BseSI GKGCMC 3 cut(s) 409, 508, 895
BseXI GCAGC 3 cut(s) 338, 339, 1170
BseYI CCCAGC 3 cut(s) 1013, 1161, 1210
BsgI GTGCAG 2 cut(s) 346, 699
BshFI GGCC 7 cut(s) 158, 302, 468, 567, 750, 1003, 1135
BshNI GGYRCC 4 cut(s) 662, 928, 949, 1268
BshVI ATCGAT 1 cut(s) 708
BsiHKAI GWGCWC 2 cut(s) 420, 508
BsiSI CCGG 1 cut(s) 701
BslFI GGGAC 2 cut(s) 77, 1171
BslI CCNNNNNNNGG 4 cut(s) 824, 1030, 1094, 1168
BsmFI GGGAC 2 cut(s) 77, 1171
BsnI GGCC 7 cut(s) 158, 302, 468, 567, 750, 1003, 1135
Bsp1286I GDGCHC 5 cut(s) 375, 409, 420, 508, 895
Bsp143I GATC 3 cut(s) 813, 843, 1288
BspACI CCGC 2 cut(s) 319, 1237
BspANI GGCC 7 cut(s) 158, 302, 468, 567, 750, 1003, 1135
BspDI ATCGAT 1 cut(s) 708
BspHI TCATGA 2 cut(s) 693, 909
BspMAI CTGCAG 1 cut(s) 1188
BspPI GGATC 2 cut(s) 808, 1283
BspQI GCTCTTC 3 cut(s) 35, 911, 1228
BspT107I GGYRCC 4 cut(s) 662, 928, 949, 1268
BsrDI GCAATG 1 cut(s) 846
BsrI ACTGG 4 cut(s) 187, 217, 289, 721
BssECI CCNNGG 5 cut(s) 408, 409, 451, 463, 945
BssMI GATC 3 cut(s) 813, 843, 1288
BssNI GRCGYC 1 cut(s) 663
BssT1I CCWWGG 1 cut(s) 451
Bst2UI CCWGG 5 cut(s) 348, 410, 465, 651, 1095
Bst4CI ACNGT 5 cut(s) 633, 745, 1009, 1124, 1250
Bst6I CTCTTC 3 cut(s) 35, 911, 1228
BstACI GRCGYC 1 cut(s) 663
BstBAI YACGTR 1 cut(s) 324
BstC8I GCNNGC 2 cut(s) 420, 752
BstDEI CTNAG 1 cut(s) 1137
BstDSI CCRYGG 1 cut(s) 945
BstEII GGTNACC 1 cut(s) 531
BstF5I GGATG 2 cut(s) 433, 1134
BstH2I RGCGCY 1 cut(s) 666
BstHHI GCGC 1 cut(s) 665
BstKTI GATC 3 cut(s) 816, 846, 1291
BstMBI GATC 3 cut(s) 813, 843, 1288
BstMWI GCNNNNNNNGC 5 cut(s) 285, 315, 324, 327, 660
BstNI CCWGG 5 cut(s) 348, 410, 465, 651, 1095
BstNSI RCATGY 2 cut(s) 316, 966
BstPI GGTNACC 1 cut(s) 531
BstSCI CCNGG 6 cut(s) 346, 408, 463, 649, 700, 1093
BstSFI CTRYAG 1 cut(s) 1184
BstSLI GKGCMC 3 cut(s) 409, 508, 895
BstV1I GCAGC 3 cut(s) 338, 339, 1170
BstXI CCANNNNNNTGG 1 cut(s) 997
Bsu15I ATCGAT 1 cut(s) 708
Bsu36I CCTNAGG 1 cut(s) 1137
BsuRI GGCC 7 cut(s) 158, 302, 468, 567, 750, 1003, 1135
BsuTUI ATCGAT 1 cut(s) 708
BtgI CCRYGG 1 cut(s) 945
BtrI CACGTC 3 cut(s) 91, 252, 1091
BtsCI GGATG 2 cut(s) 433, 1134
BtsIMutI CAGTG 3 cut(s) 296, 629, 1284
Cac8I GCNNGC 2 cut(s) 420, 752
CciI TCATGA 2 cut(s) 693, 909
CfoI GCGC 1 cut(s) 665
Cfr13I GGNCC 7 cut(s) 157, 300, 467, 683, 1001, 1158, 1242
ClaI ATCGAT 1 cut(s) 708
CseI GACGC 1 cut(s) 980
Csp6I GTAC 1 cut(s) 763
CspCI CAANNNNNGTGG 2 cut(s) 349, 384
CviAII CATG 8 cut(s) 152, 313, 694, 802, 910, 935, 963, 1051
CviQI GTAC 1 cut(s) 763
DdeI CTNAG 1 cut(s) 1137
DinI GGCGCC 1 cut(s) 664
DpnI GATC 3 cut(s) 815, 845, 1290
DpnII GATC 3 cut(s) 813, 843, 1288
Eam1104I CTCTTC 3 cut(s) 35, 911, 1228
EarI CTCTTC 3 cut(s) 35, 911, 1228
Ecl136II GAGCTC 1 cut(s) 418
Eco130I CCWWGG 1 cut(s) 451
Eco147I AGGCCT 1 cut(s) 1135
Eco24I GRGCYC 2 cut(s) 375, 420
Eco47I GGWCC 3 cut(s) 683, 1158, 1242
Eco53kI GAGCTC 1 cut(s) 418
Eco57I CTGAAG 1 cut(s) 51
Eco72I CACGTG 1 cut(s) 324
Eco81I CCTNAGG 1 cut(s) 1137
Eco91I GGTNACC 1 cut(s) 531
EcoICRI GAGCTC 1 cut(s) 418
EcoO109I RGGNCCY 3 cut(s) 683, 1001, 1158
EcoO65I GGTNACC 1 cut(s) 531
EcoRII CCWGG 5 cut(s) 346, 408, 463, 649, 1093
EcoT14I CCWWGG 1 cut(s) 451
EcoT38I GRGCYC 2 cut(s) 375, 420
EgeI GGCGCC 1 cut(s) 664
EheI GGCGCC 1 cut(s) 664
ErhI CCWWGG 1 cut(s) 451
FaeI CATG 8 cut(s) 155, 316, 697, 805, 913, 938, 966, 1054
FaqI GGGAC 2 cut(s) 77, 1171
FatI CATG 8 cut(s) 151, 312, 693, 801, 909, 934, 962, 1050
FbaI TGATCA 1 cut(s) 843
Fnu4HI GCNGC 4 cut(s) 319, 328, 352, 1184
FokI GGATG 2 cut(s) 440, 1141
FriOI GRGCYC 2 cut(s) 375, 420
Fsp4HI GCNGC 4 cut(s) 319, 328, 352, 1184
FspBI CTAG 4 cut(s) 182, 297, 563, 596
GlaI GCGC 1 cut(s) 664
GluI GCNGC 4 cut(s) 319, 328, 352, 1184
GsaI CCCAGC 3 cut(s) 1017, 1165, 1214
HaeII RGCGCY 1 cut(s) 666
HaeIII GGCC 7 cut(s) 158, 302, 468, 567, 750, 1003, 1135
HapII CCGG 1 cut(s) 701
HgaI GACGC 1 cut(s) 980
HhaI GCGC 1 cut(s) 665
Hin1I GRCGYC 1 cut(s) 663
Hin1II CATG 8 cut(s) 155, 316, 697, 805, 913, 938, 966, 1054
Hin6I GCGC 1 cut(s) 663
HinP1I GCGC 1 cut(s) 663
HincII GTYRAC 2 cut(s) 447, 726
HindII GTYRAC 2 cut(s) 447, 726
HindIII AAGCTT 1 cut(s) 110
HinfI GANTC 2 cut(s) 981, 1081
HpaII CCGG 1 cut(s) 701
HphI GGTGA 3 cut(s) 425, 639, 733
Hpy166II GTNNAC 4 cut(s) 447, 506, 726, 1120
Hpy188I TCNGA 1 cut(s) 101
Hpy188III TCNNGA 5 cut(s) 127, 376, 694, 910, 1031
Hpy8I GTNNAC 4 cut(s) 447, 506, 726, 1120
HpyAV CCTTC 3 cut(s) 23, 519, 819
HpyCH4III ACNGT 5 cut(s) 633, 745, 1009, 1124, 1250
HpyCH4IV ACGT 4 cut(s) 90, 251, 323, 1090
HpyCH4V TGCA 7 cut(s) 327, 506, 523, 629, 680, 1070, 1186
HpyF10VI GCNNNNNNNGC 5 cut(s) 285, 315, 324, 327, 660
HpyF3I CTNAG 1 cut(s) 1137
HpySE526I ACGT 4 cut(s) 90, 251, 323, 1090
Hsp92I GRCGYC 1 cut(s) 663
Hsp92II CATG 8 cut(s) 155, 316, 697, 805, 913, 938, 966, 1054
HspAI GCGC 1 cut(s) 663
KasI GGCGCC 1 cut(s) 662
KflI GGGWCCC 1 cut(s) 1158
Ksp22I TGATCA 1 cut(s) 843
Kzo9I GATC 3 cut(s) 813, 843, 1288
LguI GCTCTTC 3 cut(s) 35, 911, 1228
LmnI GCTCC 1 cut(s) 1147
Lsp1109I GCAGC 3 cut(s) 338, 339, 1170
LweI GCATC 1 cut(s) 714
MaeI CTAG 4 cut(s) 182, 297, 563, 596
MaeII ACGT 4 cut(s) 90, 251, 323, 1090
MaeIII GTNAC 4 cut(s) 391, 475, 531, 714
MalI GATC 3 cut(s) 815, 845, 1290
MboI GATC 3 cut(s) 813, 843, 1288
MboII GAAGA 6 cut(s) 22, 25, 57, 664, 898, 1215
MfeI CAATTG 2 cut(s) 232, 1146
MhlI GDGCHC 5 cut(s) 375, 409, 420, 508, 895
MluCI AATT 9 cut(s) 19, 162, 232, 576, 623, 783, 882, 1059, 1146
Mly113I GGCGCC 1 cut(s) 663
MmeI TCCRAC 1 cut(s) 1149
MseI TTAA 1 cut(s) 786
MslI CAYNNNNRTG 1 cut(s) 710
MspI CCGG 1 cut(s) 701
MspR9I CCNGG 6 cut(s) 348, 410, 465, 651, 702, 1095
MunI CAATTG 2 cut(s) 232, 1146
MvaI CCWGG 5 cut(s) 348, 410, 465, 651, 1095
MwoI GCNNNNNNNGC 5 cut(s) 285, 315, 324, 327, 660
NarI GGCGCC 1 cut(s) 663
NciI CCSGG 1 cut(s) 702
NdeII GATC 3 cut(s) 813, 843, 1288
NlaIII CATG 8 cut(s) 155, 316, 697, 805, 913, 938, 966, 1054
NmuCI GTSAC 3 cut(s) 391, 475, 714
NspI RCATGY 2 cut(s) 316, 966
PagI TCATGA 2 cut(s) 693, 909
PasI CCCWGGG 1 cut(s) 409
PceI AGGCCT 1 cut(s) 1135
PciI ACATGT 1 cut(s) 312
PciSI GCTCTTC 3 cut(s) 35, 911, 1228
PfeI GAWTC 2 cut(s) 981, 1081
PflMI CCANNNNNTGG 3 cut(s) 824, 1094, 1168
PkrI GCNGC 4 cut(s) 320, 329, 353, 1185
PluTI GGCGCC 1 cut(s) 666
PmaCI CACGTG 1 cut(s) 324
PmlI CACGTG 1 cut(s) 324
Ppu21I YACGTR 1 cut(s) 324
PpuMI RGGWCCY 2 cut(s) 683, 1158
PscI ACATGT 1 cut(s) 312
Psp124BI GAGCTC 1 cut(s) 420
Psp5II RGGWCCY 2 cut(s) 683, 1158
Psp6I CCWGG 5 cut(s) 346, 408, 463, 649, 1093
PspCI CACGTG 1 cut(s) 324
PspEI GGTNACC 1 cut(s) 531
PspFI CCCAGC 3 cut(s) 1013, 1161, 1210
PspGI CCWGG 5 cut(s) 346, 408, 463, 649, 1093
PspPI GGNCC 7 cut(s) 157, 300, 467, 683, 1001, 1158, 1242
PspPPI RGGWCCY 2 cut(s) 683, 1158
PstI CTGCAG 1 cut(s) 1188
RsaI GTAC 1 cut(s) 764
RsaNI GTAC 1 cut(s) 763
RseI CAYNNNNRTG 1 cut(s) 710
SacI GAGCTC 1 cut(s) 420
SapI GCTCTTC 3 cut(s) 35, 911, 1228
SaqAI TTAA 1 cut(s) 786
SatI GCNGC 4 cut(s) 319, 328, 352, 1184
Sau3AI GATC 3 cut(s) 813, 843, 1288
Sau96I GGNCC 7 cut(s) 157, 300, 467, 683, 1001, 1158, 1242
ScaI AGTACT 1 cut(s) 764
ScrFI CCNGG 6 cut(s) 348, 410, 465, 651, 702, 1095
SduI GDGCHC 5 cut(s) 375, 409, 420, 508, 895
SfaNI GCATC 1 cut(s) 714
SfcI CTRYAG 1 cut(s) 1184
SfoI GGCGCC 1 cut(s) 664
SinI GGWCC 3 cut(s) 683, 1158, 1242
SmiMI CAYNNNNRTG 1 cut(s) 710
SmlI CTYRAG 3 cut(s) 283, 457, 1204
SmoI CTYRAG 3 cut(s) 283, 457, 1204
Sse9I AATT 9 cut(s) 19, 162, 232, 576, 623, 783, 882, 1059, 1146
SseBI AGGCCT 1 cut(s) 1135
SsiI CCGC 2 cut(s) 319, 1237
SspDI GGCGCC 1 cut(s) 662
SspI AATATT 1 cut(s) 775
SspMI CTAG 4 cut(s) 182, 297, 563, 596
SstI GAGCTC 1 cut(s) 420
StuI AGGCCT 1 cut(s) 1135
StyD4I CCNGG 6 cut(s) 346, 408, 463, 649, 700, 1093
StyI CCWWGG 1 cut(s) 451
TaaI ACNGT 5 cut(s) 633, 745, 1009, 1124, 1250
TaiI ACGT 4 cut(s) 93, 254, 326, 1093
TaqI TCGA 3 cut(s) 708, 812, 922
TasI AATT 9 cut(s) 19, 162, 232, 576, 623, 783, 882, 1059, 1146
TatI WGTACW 1 cut(s) 762
TauI GCSGC 1 cut(s) 321
TfiI GAWTC 2 cut(s) 981, 1081
Tru1I TTAA 1 cut(s) 786
Tru9I TTAA 1 cut(s) 786
TscAI CASTG 3 cut(s) 296, 636, 1291
TseFI GTSAC 3 cut(s) 391, 475, 714
TseI GCWGC 3 cut(s) 327, 351, 1183
Tsp45I GTSAC 3 cut(s) 391, 475, 714
TspDTI ATGAA 6 cut(s) 5, 682, 710, 898, 926, 1214
TspGWI ACGGA 1 cut(s) 1281
TspRI CASTG 3 cut(s) 296, 636, 1291
Van91I CCANNNNNTGG 3 cut(s) 824, 1094, 1168
VneI GTGCAC 1 cut(s) 504
VpaK11BI GGWCC 3 cut(s) 683, 1158, 1242
XapI RAATTY 3 cut(s) 162, 623, 1059
XceI RCATGY 2 cut(s) 316, 966
XcmI CCANNNNNNNNNTGG 1 cut(s) 151
XspI CTAG 4 cut(s) 182, 297, 563, 596
ZrmI AGTACT 1 cut(s) 764
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.