Rw3G004420

Basic 7S

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0016359
Physical Location & Seq
Forward (+)
3862338 .. 3863666
1329 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G004420.1

Sequence Viewer

Length: 1227 bp
ATGGCTTCTTTCCTTCAGTTTCTTCTCTTGTTCTCTTTATCCCTTCATCTTGCTTTTGTGTCCGCGGATGATCAGTCATCTGAAACACAAACTCAACCTCGACCCCCAACGCCTATTATCAGGCCAACCAAGCTTGTTCTGAAGGTGCAGAAAGATGGTGCTACGAATCTCCATGTAGCCCAGATTTTCAAGAGAACCCCTCCAGTTCAACTCCCCTTTGTGGTAGACCTTAACGGGGAGTTCGTATCGGTCAATTGTGAGAACAAGTACTTATCATCGACTTTCAATGCTCCTATTTGCCACTCGGCCCAATGCTCTAGAGCCAACACCCACACATGCCGCACGTGCTCTCCCAAATCCCCTAGATACCCCATGGCTAGACCTGGCTGCCACGTCAATGCATGCGTGGTCACGGCTGTGAACCCAGTTACCCAACAAAGAGCCATAGACGAGCTTGCGGAGGATGTGCTTTCGATCCCATCAACCCTAGGCTCAAGGCCAGGCCCAATGGTCACAATCCCTCAATTCCTCTTTGCATGTGCATCTTCTATCCTCTTGCAAAGAGGACTACCCCAAAACCTTGCTTCCCAGTTCGGCATCCCACCGAAGATCGCCATCTGCCTCGCTTCTGGCTCGGGACAAAATGGTGTCATTTTCTTCGGCGAGGGGCCGTACATGATGCTACCGGGCATCGATTACTTGATAAACGTCCAATCCATCCAAATCAACAACAATATCGTTCCACTCCCTCCAAAATTTCGTTCTAACCAAGCCATGCTCAGCACCACAACTCCTTACACAATCCTGCATCAGTCCATCTTCATAGCTCTCACTCAACTCTACATCAAGCAGTTATCGGGAGTGCCTCAAGTGACCGCGGTGCCGCCGTTCGGGCTGTGTTTCGATGCAAACAAAATCAGTTACAGCAAGATGGGTCCGGCAGTACCTAGCATTCAGCTTGTGTTGGACAACCAGCAGAAGCTTCGGTGGACAATCTCTGGCCAAAATGTGATAGTTCAGGCACGTCCGGGAGTTATGTGCTTGGCCATGGTGAACGGCGGGCTGAACCCTAAAACTCCGATCGTGATCGGGGGACGCCAGATGGAGGATAACCTAGTGCAGCTGGATTTGGTGAACTCGAGGGTTGGCTTTAGCAACTCGTTGTTGTTCCAGAGGACCAACTGTGCCAACTTCAACTTCAACGCCAACACGACTGCTACTCCATAG

Protein Analysis

408

Amino Acids

44.35

Weight (kDa)

9.19

Isoelectric Point (pI)

40.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TAXi_N PF14543 60 - 221 1.4e-15 Xylanase inhibitor N-terminal
TAXi_C PF14541 232 - 385 1.1e-44 Xylanase inhibitor C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016359)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g05510
malus_domestica MD04G1208100.v1.1 MD12G1222100.v1.1
prunus_persica Prupe.6G329000_v2.0.a1
pyrus_communis pycom12g20830
rosa_chinensis RchiOBHm_Chr3g0454271
rosa_laevigata RLG00000025468
rosa_roxburghii Rroxscaffold_165G00437090 Rroxscaffold_6G00425250
rosa_rugosa Rorug02G0653800
rosa_samantha Rh3AG057600 Rh3BG059300 Rh3CG058500 Rh3DG059800
rosa_wichuraiana Rw3G004420

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 880
AccI GTMKAC 1 cut(s) 225
AccII CGCG 2 cut(s) 65, 878
AciI CCGC 8 cut(s) 63, 65, 340, 458, 876, 878, 884, 1059
AclWI GGATC 1 cut(s) 469
AcoI YGGCCR 2 cut(s) 1000, 1044
AcsI RAATTY 1 cut(s) 755
AcuI CTGAAG 1 cut(s) 161
AcvI CACGTG 1 cut(s) 345
AcyI GRCGYC 1 cut(s) 1096
AfaI GTAC 3 cut(s) 269, 674, 945
AfiI CCNNNNNNNGG 4 cut(s) 220, 235, 890, 1105
AgsI TTSAA 5 cut(s) 190, 209, 286, 1195, 1201
AjiI CACGTC 2 cut(s) 394, 1025
AjnI CCWGG 2 cut(s) 382, 499
AjuI GAANNNNNNNTTGG 4 cut(s) 745, 777, 1181, 1213
AleI CACNNNNGTG 1 cut(s) 416
AluBI AGCT 6 cut(s) 133, 454, 827, 958, 982, 1123
AluI AGCT 6 cut(s) 133, 454, 827, 958, 982, 1123
Alw21I GWGCWC 1 cut(s) 350
AlwI GGATC 1 cut(s) 469
Ama87I CYCGRG 2 cut(s) 634, 1138
AoxI GGCC 7 cut(s) 122, 306, 497, 502, 668, 1000, 1044
ApeKI GCWGC 2 cut(s) 387, 1120
ApoI RAATTY 1 cut(s) 755
AspA2I CCTAGG 1 cut(s) 487
AspS9I GGNCC 5 cut(s) 307, 503, 668, 935, 1176
AsuC2I CCSGG 2 cut(s) 687, 1029
AsuHPI GGTGA 2 cut(s) 1063, 1144
AvaI CYCGRG 2 cut(s) 634, 1138
AvaII GGWCC 2 cut(s) 935, 1176
AvrII CCTAGG 1 cut(s) 487
BalI TGGCCA 2 cut(s) 1002, 1046
BanI GGYRCC 1 cut(s) 880
BbrPI CACGTG 1 cut(s) 345
Bbv12I GWGCWC 1 cut(s) 350
BbvI GCAGC 2 cut(s) 374, 1132
BccI CCATC 7 cut(s) 149, 487, 623, 725, 824, 925, 1096
BceAI ACGGC 4 cut(s) 429, 655, 871, 1072
BcgI CGANNNNNNTGC 2 cut(s) 965, 999
BciT130I CCWGG 2 cut(s) 384, 501
BclI TGATCA 1 cut(s) 70
BcnI CCSGG 2 cut(s) 687, 1029
BfaI CTAG 6 cut(s) 318, 363, 378, 488, 948, 1115
BglI GCCNNNNNGGC 1 cut(s) 892
BisI GCNGC 4 cut(s) 340, 388, 884, 1121
BlnI CCTAGG 1 cut(s) 487
BlpI GCTNAGC 1 cut(s) 779
BlsI GCNGC 4 cut(s) 341, 389, 885, 1122
BmcAI AGTACT 1 cut(s) 269
Bme1390I CCNGG 4 cut(s) 384, 501, 687, 1029
Bme18I GGWCC 2 cut(s) 935, 1176
BmeT110I CYCGRG 2 cut(s) 634, 1138
BmgBI CACGTC 2 cut(s) 394, 1025
BmgT120I GGNCC 5 cut(s) 307, 503, 668, 935, 1176
BmiI GGNNCC 3 cut(s) 669, 882, 936
BmrFI CCNGG 4 cut(s) 384, 501, 687, 1029
BmrI ACTGGG 2 cut(s) 419, 583
BmsI GCATC 6 cut(s) 551, 606, 669, 699, 817, 895
BmuI ACTGGG 2 cut(s) 419, 583
BplI GAGNNNNNCTC 2 cut(s) 184, 216
BpmI CTGGAG 1 cut(s) 186
Bpu1102I GCTNAGC 1 cut(s) 779
BpuEI CTTGAG 2 cut(s) 478, 852
BpuMI CCSGG 2 cut(s) 687, 1029
Bsa29I ATCGAT 1 cut(s) 693
BsaAI YACGTR 1 cut(s) 345
BsaBI GATNNNNATC 2 cut(s) 614, 1085
BsaHI GRCGYC 1 cut(s) 1096
BsaJI CCNNGG 5 cut(s) 63, 372, 487, 876, 1047
BsaXI ACNNNNNCTCC 5 cut(s) 334, 364, 775, 805, 1204
Bsc4I CCNNNNNNNGG 4 cut(s) 220, 235, 890, 1105
Bse1I ACTGG 3 cut(s) 203, 425, 589
Bse8I GATNNNNATC 2 cut(s) 614, 1085
BseBI CCWGG 2 cut(s) 384, 501
BseCI ATCGAT 1 cut(s) 693
BseDI CCNNGG 5 cut(s) 63, 372, 487, 876, 1047
BseGI GGATG 4 cut(s) 73, 469, 597, 717
BseJI GATNNNNATC 2 cut(s) 614, 1085
BseLI CCNNNNNNNGG 4 cut(s) 220, 235, 890, 1105
BseMII CTCAG 1 cut(s) 793
BseNI ACTGG 3 cut(s) 203, 425, 589
BseXI GCAGC 2 cut(s) 374, 1132
BsgI GTGCAG 2 cut(s) 167, 1139
Bsh1236I CGCG 2 cut(s) 65, 878
Bsh1285I CGRYCG 1 cut(s) 1083
BshFI GGCC 7 cut(s) 124, 308, 499, 504, 670, 1002, 1046
BshNI GGYRCC 1 cut(s) 880
BshVI ATCGAT 1 cut(s) 693
BsiEI CGRYCG 1 cut(s) 1083
BsiHKAI GWGCWC 1 cut(s) 350
BsiHKCI CYCGRG 2 cut(s) 634, 1138
BsiSI CCGG 3 cut(s) 686, 938, 1028
BslFI GGGAC 2 cut(s) 651, 1107
BslI CCNNNNNNNGG 4 cut(s) 220, 235, 890, 1105
BsmFI GGGAC 2 cut(s) 651, 1107
BsmI GAATGC 1 cut(s) 951
BsnI GGCC 7 cut(s) 124, 308, 499, 504, 670, 1002, 1046
BsoBI CYCGRG 2 cut(s) 634, 1138
Bsp1286I GDGCHC 1 cut(s) 350
Bsp143I GATC 5 cut(s) 70, 474, 609, 1080, 1086
Bsp1720I GCTNAGC 1 cut(s) 779
Bsp19I CCATGG 2 cut(s) 372, 1047
BspACI CCGC 8 cut(s) 63, 65, 340, 458, 876, 878, 884, 1059
BspANI GGCC 7 cut(s) 124, 308, 499, 504, 670, 1002, 1046
BspCNI CTCAG 1 cut(s) 792
BspDI ATCGAT 1 cut(s) 693
BspFNI CGCG 2 cut(s) 65, 878
BspLI GGNNCC 3 cut(s) 669, 882, 936
BspPI GGATC 1 cut(s) 469
BspT107I GGYRCC 1 cut(s) 880
BsrI ACTGG 3 cut(s) 203, 425, 589
BssECI CCNNGG 5 cut(s) 63, 372, 487, 876, 1047
BssMI GATC 5 cut(s) 70, 474, 609, 1080, 1086
BssNI GRCGYC 1 cut(s) 1096
BssT1I CCWWGG 3 cut(s) 372, 487, 1047
Bst2UI CCWGG 2 cut(s) 384, 501
Bst4CI ACNGT 1 cut(s) 1184
BstACI GRCGYC 1 cut(s) 1096
BstBAI YACGTR 1 cut(s) 345
BstC8I GCNNGC 3 cut(s) 403, 456, 1061
BstDEI CTNAG 1 cut(s) 779
BstDSI CCRYGG 4 cut(s) 63, 372, 876, 1047
BstF5I GGATG 4 cut(s) 73, 469, 597, 717
BstFNI CGCG 2 cut(s) 65, 878
BstKTI GATC 5 cut(s) 73, 477, 612, 1083, 1089
BstMBI GATC 5 cut(s) 70, 474, 609, 1080, 1086
BstMCI CGRYCG 1 cut(s) 1083
BstMWI GCNNNNNNNGC 3 cut(s) 130, 345, 892
BstNI CCWGG 2 cut(s) 384, 501
BstNSI RCATGY 3 cut(s) 339, 405, 540
BstSCI CCNGG 4 cut(s) 382, 499, 685, 1027
BstUI CGCG 2 cut(s) 65, 878
BstV1I GCAGC 2 cut(s) 374, 1132
Bsu15I ATCGAT 1 cut(s) 693
BsuRI GGCC 7 cut(s) 124, 308, 499, 504, 670, 1002, 1046
BsuTUI ATCGAT 1 cut(s) 693
BtgI CCRYGG 4 cut(s) 63, 372, 876, 1047
BtrI CACGTC 2 cut(s) 394, 1025
BtsCI GGATG 4 cut(s) 73, 469, 597, 717
Cac8I GCNNGC 3 cut(s) 403, 456, 1061
Cfr13I GGNCC 5 cut(s) 307, 503, 668, 935, 1176
Cfr42I CCGCGG 2 cut(s) 66, 879
ClaI ATCGAT 1 cut(s) 693
CseI GACGC 1 cut(s) 1104
Csp6I GTAC 3 cut(s) 268, 673, 944
CviAII CATG 8 cut(s) 173, 336, 373, 402, 537, 676, 775, 1048
CviQI GTAC 3 cut(s) 268, 673, 944
DdeI CTNAG 1 cut(s) 779
DpnI GATC 5 cut(s) 72, 476, 611, 1082, 1088
DpnII GATC 5 cut(s) 70, 474, 609, 1080, 1086
EaeI YGGCCR 2 cut(s) 1000, 1044
Eco130I CCWWGG 3 cut(s) 372, 487, 1047
Eco47I GGWCC 2 cut(s) 935, 1176
Eco57I CTGAAG 1 cut(s) 161
Eco72I CACGTG 1 cut(s) 345
Eco88I CYCGRG 2 cut(s) 634, 1138
EcoRII CCWGG 2 cut(s) 382, 499
EcoT14I CCWWGG 3 cut(s) 372, 487, 1047
EcoT22I ATGCAT 1 cut(s) 403
ErhI CCWWGG 3 cut(s) 372, 487, 1047
FaeI CATG 8 cut(s) 176, 339, 376, 405, 540, 679, 778, 1051
FaqI GGGAC 2 cut(s) 651, 1107
FatI CATG 8 cut(s) 172, 335, 372, 401, 536, 675, 774, 1047
FauI CCCGC 1 cut(s) 1052
FbaI TGATCA 1 cut(s) 70
FblI GTMKAC 1 cut(s) 225
Fnu4HI GCNGC 4 cut(s) 340, 388, 884, 1121
FokI GGATG 4 cut(s) 80, 476, 584, 704
Fsp4HI GCNGC 4 cut(s) 340, 388, 884, 1121
FspBI CTAG 6 cut(s) 318, 363, 378, 488, 948, 1115
GluI GCNGC 4 cut(s) 340, 388, 884, 1121
GsuI CTGGAG 1 cut(s) 186
HaeIII GGCC 7 cut(s) 124, 308, 499, 504, 670, 1002, 1046
HapII CCGG 3 cut(s) 686, 938, 1028
HgaI GACGC 1 cut(s) 1104
Hin1I GRCGYC 1 cut(s) 1096
Hin1II CATG 8 cut(s) 176, 339, 376, 405, 540, 679, 778, 1051
HindIII AAGCTT 2 cut(s) 131, 980
HinfI GANTC 1 cut(s) 166
HpaII CCGG 3 cut(s) 686, 938, 1028
HphI GGTGA 2 cut(s) 1063, 1144
Hpy166II GTNNAC 5 cut(s) 226, 421, 990, 1054, 1135
Hpy188I TCNGA 3 cut(s) 82, 141, 1080
Hpy188III TCNNGA 6 cut(s) 190, 318, 636, 858, 1084, 1171
Hpy8I GTNNAC 5 cut(s) 226, 421, 990, 1054, 1135
HpyAV CCTTC 3 cut(s) 23, 53, 136
HpyCH4III ACNGT 1 cut(s) 1184
HpyCH4IV ACGT 4 cut(s) 344, 393, 708, 1024
HpyCH4V TGCA 8 cut(s) 148, 401, 536, 542, 559, 808, 908, 1120
HpyF10VI GCNNNNNNNGC 3 cut(s) 130, 345, 892
HpyF3I CTNAG 1 cut(s) 779
HpySE526I ACGT 4 cut(s) 344, 393, 708, 1024
Hsp92I GRCGYC 1 cut(s) 1096
Hsp92II CATG 8 cut(s) 176, 339, 376, 405, 540, 679, 778, 1051
Ksp22I TGATCA 1 cut(s) 70
KspI CCGCGG 2 cut(s) 66, 879
Kzo9I GATC 5 cut(s) 70, 474, 609, 1080, 1086
LmnI GCTCC 1 cut(s) 295
Lsp1109I GCAGC 2 cut(s) 374, 1132
LweI GCATC 6 cut(s) 551, 606, 669, 699, 817, 895
MaeI CTAG 6 cut(s) 318, 363, 378, 488, 948, 1115
MaeII ACGT 4 cut(s) 344, 393, 708, 1024
MaeIII GTNAC 5 cut(s) 409, 427, 511, 871, 920
MalI GATC 5 cut(s) 72, 476, 611, 1082, 1088
MboI GATC 5 cut(s) 70, 474, 609, 1080, 1086
MboII GAAGA 5 cut(s) 14, 537, 619, 649, 811
MfeI CAATTG 1 cut(s) 253
MhlI GDGCHC 1 cut(s) 350
MlsI TGGCCA 2 cut(s) 1002, 1046
MluCI AATT 3 cut(s) 253, 524, 755
MluNI TGGCCA 2 cut(s) 1002, 1046
MmeI TCCRAC 1 cut(s) 945
Mox20I TGGCCA 2 cut(s) 1002, 1046
Mph1103I ATGCAT 1 cut(s) 403
MscI TGGCCA 2 cut(s) 1002, 1046
MseI TTAA 1 cut(s) 231
MslI CAYNNNNRTG 2 cut(s) 396, 416
Msp20I TGGCCA 2 cut(s) 1002, 1046
MspA1I CMGCKG 3 cut(s) 65, 878, 1123
MspI CCGG 3 cut(s) 686, 938, 1028
MspR9I CCNGG 4 cut(s) 384, 501, 687, 1029
MunI CAATTG 1 cut(s) 253
Mva1269I GAATGC 1 cut(s) 951
MvaI CCWGG 2 cut(s) 384, 501
MvnI CGCG 2 cut(s) 65, 878
MwoI GCNNNNNNNGC 3 cut(s) 130, 345, 892
NciI CCSGG 2 cut(s) 687, 1029
NcoI CCATGG 2 cut(s) 372, 1047
NdeII GATC 5 cut(s) 70, 474, 609, 1080, 1086
NlaIII CATG 8 cut(s) 176, 339, 376, 405, 540, 679, 778, 1051
NlaIV GGNNCC 3 cut(s) 669, 882, 936
NmeAIII GCCGAG 1 cut(s) 284
NmuCI GTSAC 3 cut(s) 409, 511, 871
NsiI ATGCAT 1 cut(s) 403
NspI RCATGY 3 cut(s) 339, 405, 540
OliI CACNNNNGTG 1 cut(s) 416
PaeI GCATGC 1 cut(s) 405
PaeR7I CTCGAG 1 cut(s) 1138
PcsI WCGNNNNNNNCGW 1 cut(s) 240
PctI GAATGC 1 cut(s) 951
PfeI GAWTC 1 cut(s) 166
PfoI TCCNGGA 1 cut(s) 1027
PkrI GCNGC 4 cut(s) 341, 389, 885, 1122
Ple19I CGATCG 1 cut(s) 1083
PmaCI CACGTG 1 cut(s) 345
PmlI CACGTG 1 cut(s) 345
Ppu21I YACGTR 1 cut(s) 345
Psp6I CCWGG 2 cut(s) 382, 499
PspCI CACGTG 1 cut(s) 345
PspGI CCWGG 2 cut(s) 382, 499
PspN4I GGNNCC 3 cut(s) 669, 882, 936
PspPI GGNCC 5 cut(s) 307, 503, 668, 935, 1176
PspXI VCTCGAGB 1 cut(s) 1138
PvuI CGATCG 1 cut(s) 1083
PvuII CAGCTG 1 cut(s) 1123
RsaI GTAC 3 cut(s) 269, 674, 945
RsaNI GTAC 3 cut(s) 268, 673, 944
RseI CAYNNNNRTG 2 cut(s) 396, 416
SacII CCGCGG 2 cut(s) 66, 879
SaqAI TTAA 1 cut(s) 231
SatI GCNGC 4 cut(s) 340, 388, 884, 1121
Sau3AI GATC 5 cut(s) 70, 474, 609, 1080, 1086
Sau96I GGNCC 5 cut(s) 307, 503, 668, 935, 1176
ScaI AGTACT 1 cut(s) 269
ScrFI CCNGG 4 cut(s) 384, 501, 687, 1029
SduI GDGCHC 1 cut(s) 350
SfaNI GCATC 6 cut(s) 551, 606, 669, 699, 817, 895
Sfr274I CTCGAG 1 cut(s) 1138
Sfr303I CCGCGG 2 cut(s) 66, 879
SgrBI CCGCGG 2 cut(s) 66, 879
SinI GGWCC 2 cut(s) 935, 1176
SlaI CTCGAG 1 cut(s) 1138
SmiMI CAYNNNNRTG 2 cut(s) 396, 416
SmlI CTYRAG 3 cut(s) 493, 867, 1138
SmoI CTYRAG 3 cut(s) 493, 867, 1138
SphI GCATGC 1 cut(s) 405
Sse9I AATT 3 cut(s) 253, 524, 755
SsiI CCGC 8 cut(s) 63, 65, 340, 458, 876, 878, 884, 1059
SspMI CTAG 6 cut(s) 318, 363, 378, 488, 948, 1115
StyD4I CCNGG 4 cut(s) 382, 499, 685, 1027
StyI CCWWGG 3 cut(s) 372, 487, 1047
TaaI ACNGT 1 cut(s) 1184
TaiI ACGT 4 cut(s) 347, 396, 711, 1027
TaqI TCGA 6 cut(s) 100, 278, 473, 693, 903, 1139
TaqII GACCGA 1 cut(s) 238
TasI AATT 3 cut(s) 253, 524, 755
TatI WGTACW 1 cut(s) 267
TauI GCSGC 2 cut(s) 342, 886
TfiI GAWTC 1 cut(s) 166
Tru1I TTAA 1 cut(s) 231
Tru9I TTAA 1 cut(s) 231
TseFI GTSAC 3 cut(s) 409, 511, 871
TseI GCWGC 2 cut(s) 387, 1120
Tsp45I GTSAC 3 cut(s) 409, 511, 871
TspDTI ATGAA 2 cut(s) 35, 811
VpaK11BI GGWCC 2 cut(s) 935, 1176
XapI RAATTY 1 cut(s) 755
XbaI TCTAGA 1 cut(s) 317
XceI RCATGY 3 cut(s) 339, 405, 540
XhoI CTCGAG 1 cut(s) 1138
XmaJI CCTAGG 1 cut(s) 487
XmiI GTMKAC 1 cut(s) 225
XspI CTAG 6 cut(s) 318, 363, 378, 488, 948, 1115
ZrmI AGTACT 1 cut(s) 269
Zsp2I ATGCAT 1 cut(s) 403
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.