pycom12g20830

Basic 7S

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0016359
Physical Location & Seq
Reverse (-)
22170411 .. 22171334
924 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12g20830.1

Sequence Viewer

Length: 924 bp
ATGGCTTCTTTCCTTCCCAATTTGCTCCTACTCTTCTCTTGCTTTTCCCTTCTCATTTTCACGTCTGTGGCGACAGTAAAAACCCGACCCCAAATCCCCATCAAGCCGGATAAGCTTGTCCTCAAGGTGCAGAAAGATGGGGCAACCAATCTTCATGTGGCCCAAATTTACAAAAGAAACCCTCCAGTCCAATTCCCCTTTGTGGTTGACTTAAATGGAAGGTTCTTATCAGTCAATTGTGAGAACCAGTACTTGTCATCGGGCTACAATGCTCCGGTTTGCCACTCAACACAGTGTGCTAGAGCCAATCCTAATAGCCAGATGACATGCCGCACATGCAGCTCATCTAGAACCCCACTAGGGTGCCACTCCAACGCGTGTGGGCTCATGACCACAAACCCTGTGACTCGCCAAAGCGCTATGGGTGAGCTTGCTCAAGATGTCCTCTCAATCCCATCAACCCAAGGCTCAAGCCCTGGCCCAATGGTCCAAGTCCCTCAATTCCTCTTTGCATGTGCACCTTCAAATATTATGCAAAAAGGCCTACCCAAAAATGTTCAAGGTTTTGCTGGCCTAGGCCACTCCCCAATTTCCCTACCCTACCAACTAGCCTCCCACTTCGGCTTTCCAGCAAAATTTGCCGTGTGCCTCCCTTCTGCGCCTGGCCAGAATGGCGTCATTTTCTTCGGGGAAGGACCCTATCTCATGAACCGGGGCATCGACGTGTCGCGCAAGTTGACCTACACCCCACTGACCATAGGCAGGCAAGGAGAGTACTACATAAACGTTCAATCCGTCAAAATCAACAACAAGGTGGTTCCACTCAACACATCTCTCTTGCCCTACTCACCAAAACGAGGGGTTGGTGGGACAATGATCGAGCACCACAACTCCCTACACAATCCTCCAAACCTCAATCTTTAG

Protein Analysis

308

Amino Acids

33.27

Weight (kDa)

9.51

Isoelectric Point (pI)

41.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TAXi_N PF14543 54 - 230 4.5e-35 Xylanase inhibitor N-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016359)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g05510
malus_domestica MD04G1208100.v1.1 MD12G1222100.v1.1
prunus_persica Prupe.6G329000_v2.0.a1
pyrus_communis pycom12g20830
rosa_chinensis RchiOBHm_Chr3g0454271
rosa_laevigata RLG00000025468
rosa_roxburghii Rroxscaffold_165G00437090 Rroxscaffold_6G00425250
rosa_rugosa Rorug02G0653800
rosa_samantha Rh3AG057600 Rh3BG059300 Rh3CG058500 Rh3DG059800
rosa_wichuraiana Rw3G004420

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 363
AccII CGCG 2 cut(s) 377, 730
AciI CCGC 1 cut(s) 331
AclI AACGTT 1 cut(s) 786
AcoI YGGCCR 1 cut(s) 664
AcsI RAATTY 2 cut(s) 165, 635
AcyI GRCGYC 1 cut(s) 675
AdeI CACNNNGTG 1 cut(s) 296
AfaI GTAC 2 cut(s) 251, 776
AfeI AGCGCT 1 cut(s) 418
AfiI CCNNNNNNNGG 4 cut(s) 202, 360, 762, 857
AflIII ACRYGT 2 cut(s) 375, 723
AgsI TTSAA 3 cut(s) 525, 560, 791
AjiI CACGTC 2 cut(s) 63, 724
AjnI CCWGG 2 cut(s) 475, 661
AleI CACNNNNGTG 2 cut(s) 65, 361
AluBI AGCT 3 cut(s) 115, 342, 430
AluI AGCT 3 cut(s) 115, 342, 430
Alw21I GWGCWC 2 cut(s) 520, 885
Alw44I GTGCAC 1 cut(s) 516
Aor51HI AGCGCT 1 cut(s) 418
AoxI GGCC 6 cut(s) 159, 478, 541, 571, 577, 664
ApaLI GTGCAC 1 cut(s) 516
ApeKI GCWGC 1 cut(s) 339
ApoI RAATTY 2 cut(s) 165, 635
AspA2I CCTAGG 1 cut(s) 574
AspLEI GCGC 3 cut(s) 419, 661, 732
AspS9I GGNCC 4 cut(s) 160, 479, 487, 695
AsuC2I CCSGG 1 cut(s) 713
AsuHPI GGTGA 2 cut(s) 437, 840
AvaII GGWCC 2 cut(s) 487, 695
AvrII CCTAGG 1 cut(s) 574
BaeGI GKGCMC 1 cut(s) 520
BalI TGGCCA 1 cut(s) 666
BanI GGYRCC 1 cut(s) 363
BanII GRGCYC 1 cut(s) 387
Bbv12I GWGCWC 2 cut(s) 520, 885
BbvI GCAGC 1 cut(s) 351
BccI CCATC 3 cut(s) 107, 131, 463
BceAI ACGGC 1 cut(s) 626
BciT130I CCWGG 2 cut(s) 477, 663
BcnI CCSGG 1 cut(s) 713
BfaI CTAG 5 cut(s) 300, 348, 359, 575, 608
BfoI RGCGCY 1 cut(s) 420
BglI GCCNNNNNGGC 1 cut(s) 672
BisI GCNGC 2 cut(s) 331, 340
BlnI CCTAGG 1 cut(s) 574
BlsI GCNGC 2 cut(s) 332, 341
BmcAI AGTACT 2 cut(s) 251, 776
Bme1390I CCNGG 3 cut(s) 477, 663, 713
Bme18I GGWCC 2 cut(s) 487, 695
BmgBI CACGTC 2 cut(s) 63, 724
BmgT120I GGNCC 4 cut(s) 160, 479, 487, 695
BmiI GGNNCC 3 cut(s) 365, 697, 819
BmrFI CCNGG 3 cut(s) 477, 663, 713
BmsI GCATC 1 cut(s) 726
BpmI CTGGAG 1 cut(s) 168
BpuEI CTTGAG 3 cut(s) 107, 420, 454
BpuMI CCSGG 1 cut(s) 713
BsaHI GRCGYC 1 cut(s) 675
BsaJI CCNNGG 4 cut(s) 463, 475, 574, 712
BsaWI WCCGGW 1 cut(s) 274
BsaXI ACNNNNNCTCC 2 cut(s) 875, 905
Bsc4I CCNNNNNNNGG 4 cut(s) 202, 360, 762, 857
Bse1I ACTGG 2 cut(s) 185, 247
BseBI CCWGG 2 cut(s) 477, 663
BseDI CCNNGG 4 cut(s) 463, 475, 574, 712
BseLI CCNNNNNNNGG 4 cut(s) 202, 360, 762, 857
BseNI ACTGG 2 cut(s) 185, 247
BseSI GKGCMC 1 cut(s) 520
BseXI GCAGC 1 cut(s) 351
BsgI GTGCAG 1 cut(s) 149
Bsh1236I CGCG 2 cut(s) 377, 730
BshFI GGCC 6 cut(s) 161, 480, 543, 573, 579, 666
BshNI GGYRCC 1 cut(s) 363
BsiHKAI GWGCWC 2 cut(s) 520, 885
BsiSI CCGG 3 cut(s) 107, 275, 712
BslFI GGGAC 2 cut(s) 479, 883
BslI CCNNNNNNNGG 4 cut(s) 202, 360, 762, 857
BsmFI GGGAC 2 cut(s) 479, 883
BsnI GGCC 6 cut(s) 161, 480, 543, 573, 579, 666
Bsp1286I GDGCHC 3 cut(s) 387, 520, 885
Bsp143I GATC 1 cut(s) 876
BspACI CCGC 1 cut(s) 331
BspANI GGCC 6 cut(s) 161, 480, 543, 573, 579, 666
BspFNI CGCG 2 cut(s) 377, 730
BspHI TCATGA 2 cut(s) 387, 705
BspLI GGNNCC 3 cut(s) 365, 697, 819
BspT107I GGYRCC 1 cut(s) 363
BsrI ACTGG 2 cut(s) 185, 247
BssECI CCNNGG 4 cut(s) 463, 475, 574, 712
BssMI GATC 1 cut(s) 876
BssNI GRCGYC 1 cut(s) 675
BssT1I CCWWGG 2 cut(s) 463, 574
Bst2UI CCWGG 2 cut(s) 477, 663
Bst4CI ACNGT 2 cut(s) 76, 294
Bst6I CTCTTC 1 cut(s) 38
BstACI GRCGYC 1 cut(s) 675
BstAPI GCANNNNNTGC 1 cut(s) 638
BstC8I GCNNGC 3 cut(s) 432, 571, 764
BstFNI CGCG 2 cut(s) 377, 730
BstH2I RGCGCY 1 cut(s) 420
BstHHI GCGC 3 cut(s) 419, 661, 732
BstKTI GATC 1 cut(s) 879
BstMBI GATC 1 cut(s) 876
BstMWI GCNNNNNNNGC 5 cut(s) 112, 336, 339, 638, 672
BstNI CCWGG 2 cut(s) 477, 663
BstNSI RCATGY 3 cut(s) 330, 339, 516
BstSCI CCNGG 3 cut(s) 475, 661, 711
BstSLI GKGCMC 1 cut(s) 520
BstUI CGCG 2 cut(s) 377, 730
BstV1I GCAGC 1 cut(s) 351
BsuRI GGCC 6 cut(s) 161, 480, 543, 573, 579, 666
BtrI CACGTC 2 cut(s) 63, 724
BtsIMutI CAGTG 2 cut(s) 299, 749
Cac8I GCNNGC 3 cut(s) 432, 571, 764
CciI TCATGA 2 cut(s) 387, 705
CfoI GCGC 3 cut(s) 419, 661, 732
Cfr13I GGNCC 4 cut(s) 160, 479, 487, 695
CseI GACGC 1 cut(s) 664
Csp6I GTAC 2 cut(s) 250, 775
CspCI CAANNNNNGTGG 2 cut(s) 361, 396
CviAII CATG 6 cut(s) 155, 327, 336, 388, 513, 706
CviQI GTAC 2 cut(s) 250, 775
DpnI GATC 1 cut(s) 878
DpnII GATC 1 cut(s) 876
DraIII CACNNNGTG 1 cut(s) 296
EaeI YGGCCR 1 cut(s) 664
Eam1104I CTCTTC 1 cut(s) 38
EarI CTCTTC 1 cut(s) 38
Eco130I CCWWGG 2 cut(s) 463, 574
Eco147I AGGCCT 1 cut(s) 543
Eco24I GRGCYC 1 cut(s) 387
Eco47I GGWCC 2 cut(s) 487, 695
Eco47III AGCGCT 1 cut(s) 418
EcoO109I RGGNCCY 1 cut(s) 695
EcoRII CCWGG 2 cut(s) 475, 661
EcoT14I CCWWGG 2 cut(s) 463, 574
EcoT38I GRGCYC 1 cut(s) 387
ErhI CCWWGG 2 cut(s) 463, 574
FaeI CATG 6 cut(s) 158, 330, 339, 391, 516, 709
FaqI GGGAC 2 cut(s) 479, 883
FatI CATG 6 cut(s) 154, 326, 335, 387, 512, 705
Fnu4HI GCNGC 2 cut(s) 331, 340
FriOI GRGCYC 1 cut(s) 387
Fsp4HI GCNGC 2 cut(s) 331, 340
FspBI CTAG 5 cut(s) 300, 348, 359, 575, 608
GlaI GCGC 3 cut(s) 418, 660, 731
GluI GCNGC 2 cut(s) 331, 340
GsuI CTGGAG 1 cut(s) 168
HaeII RGCGCY 1 cut(s) 420
HaeIII GGCC 6 cut(s) 161, 480, 543, 573, 579, 666
HapII CCGG 3 cut(s) 107, 275, 712
HgaI GACGC 1 cut(s) 664
HhaI GCGC 3 cut(s) 419, 661, 732
Hin1I GRCGYC 1 cut(s) 675
Hin1II CATG 6 cut(s) 158, 330, 339, 391, 516, 709
Hin6I GCGC 3 cut(s) 417, 659, 730
HinP1I GCGC 3 cut(s) 417, 659, 730
HincII GTYRAC 2 cut(s) 208, 738
HindII GTYRAC 2 cut(s) 208, 738
HindIII AAGCTT 1 cut(s) 113
HinfI GANTC 1 cut(s) 406
HpaII CCGG 3 cut(s) 107, 275, 712
HphI GGTGA 2 cut(s) 437, 840
Hpy166II GTNNAC 3 cut(s) 208, 518, 738
Hpy188III TCNNGA 4 cut(s) 348, 388, 437, 706
Hpy8I GTNNAC 3 cut(s) 208, 518, 738
Hpy99I CGWCG 1 cut(s) 725
HpyAV CCTTC 6 cut(s) 23, 59, 213, 531, 663, 686
HpyCH4III ACNGT 2 cut(s) 76, 294
HpyCH4IV ACGT 3 cut(s) 62, 723, 786
HpyCH4V TGCA 5 cut(s) 130, 339, 512, 518, 535
HpyF10VI GCNNNNNNNGC 5 cut(s) 112, 336, 339, 638, 672
HpySE526I ACGT 3 cut(s) 62, 723, 786
Hsp92I GRCGYC 1 cut(s) 675
Hsp92II CATG 6 cut(s) 158, 330, 339, 391, 516, 709
HspAI GCGC 3 cut(s) 417, 659, 730
Kzo9I GATC 1 cut(s) 876
LmnI GCTCC 2 cut(s) 30, 277
Lsp1109I GCAGC 1 cut(s) 351
LweI GCATC 1 cut(s) 726
MaeI CTAG 5 cut(s) 300, 348, 359, 575, 608
MaeII ACGT 3 cut(s) 62, 723, 786
MaeIII GTNAC 1 cut(s) 403
MalI GATC 1 cut(s) 878
MboI GATC 1 cut(s) 876
MboII GAAGA 3 cut(s) 25, 143, 676
MfeI CAATTG 1 cut(s) 235
MhlI GDGCHC 3 cut(s) 387, 520, 885
MlsI TGGCCA 1 cut(s) 666
MluCI AATT 7 cut(s) 19, 165, 191, 235, 500, 588, 635
MluI ACGCGT 1 cut(s) 375
MluNI TGGCCA 1 cut(s) 666
MlyI GAGTC 1 cut(s) 400
MmeI TCCRAC 1 cut(s) 396
Mox20I TGGCCA 1 cut(s) 666
MscI TGGCCA 1 cut(s) 666
MseI TTAA 1 cut(s) 212
MslI CAYNNNNRTG 3 cut(s) 65, 361, 722
Msp20I TGGCCA 1 cut(s) 666
MspI CCGG 3 cut(s) 107, 275, 712
MspR9I CCNGG 3 cut(s) 477, 663, 713
MunI CAATTG 1 cut(s) 235
MvaI CCWGG 2 cut(s) 477, 663
MvnI CGCG 2 cut(s) 377, 730
MwoI GCNNNNNNNGC 5 cut(s) 112, 336, 339, 638, 672
NciI CCSGG 1 cut(s) 713
NdeII GATC 1 cut(s) 876
NlaIII CATG 6 cut(s) 158, 330, 339, 391, 516, 709
NlaIV GGNNCC 3 cut(s) 365, 697, 819
NmuCI GTSAC 1 cut(s) 403
NspI RCATGY 3 cut(s) 330, 339, 516
OliI CACNNNNGTG 2 cut(s) 65, 361
PagI TCATGA 2 cut(s) 387, 705
PceI AGGCCT 1 cut(s) 543
PcsI WCGNNNNNNNCGW 2 cut(s) 68, 792
PkrI GCNGC 2 cut(s) 332, 341
PleI GAGTC 1 cut(s) 400
PpsI GAGTC 1 cut(s) 400
PpuMI RGGWCCY 1 cut(s) 695
Psp1406I AACGTT 1 cut(s) 786
Psp5II RGGWCCY 1 cut(s) 695
Psp6I CCWGG 2 cut(s) 475, 661
PspGI CCWGG 2 cut(s) 475, 661
PspN4I GGNNCC 3 cut(s) 365, 697, 819
PspPI GGNCC 4 cut(s) 160, 479, 487, 695
PspPPI RGGWCCY 1 cut(s) 695
RsaI GTAC 2 cut(s) 251, 776
RsaNI GTAC 2 cut(s) 250, 775
RseI CAYNNNNRTG 3 cut(s) 65, 361, 722
SaqAI TTAA 1 cut(s) 212
SatI GCNGC 2 cut(s) 331, 340
Sau3AI GATC 1 cut(s) 876
Sau96I GGNCC 4 cut(s) 160, 479, 487, 695
ScaI AGTACT 2 cut(s) 251, 776
SchI GAGTC 1 cut(s) 400
ScrFI CCNGG 3 cut(s) 477, 663, 713
SduI GDGCHC 3 cut(s) 387, 520, 885
SfaNI GCATC 1 cut(s) 726
SinI GGWCC 2 cut(s) 487, 695
SmiMI CAYNNNNRTG 3 cut(s) 65, 361, 722
SmlI CTYRAG 3 cut(s) 122, 435, 469
SmoI CTYRAG 3 cut(s) 122, 435, 469
Sse9I AATT 7 cut(s) 19, 165, 191, 235, 500, 588, 635
SseBI AGGCCT 1 cut(s) 543
SsiI CCGC 1 cut(s) 331
SspI AATATT 1 cut(s) 529
SspMI CTAG 5 cut(s) 300, 348, 359, 575, 608
StuI AGGCCT 1 cut(s) 543
StyD4I CCNGG 3 cut(s) 475, 661, 711
StyI CCWWGG 2 cut(s) 463, 574
TaaI ACNGT 2 cut(s) 76, 294
TaiI ACGT 3 cut(s) 65, 726, 789
TaqI TCGA 2 cut(s) 720, 879
TasI AATT 7 cut(s) 19, 165, 191, 235, 500, 588, 635
TatI WGTACW 2 cut(s) 249, 774
TauI GCSGC 1 cut(s) 333
Tru1I TTAA 1 cut(s) 212
Tru9I TTAA 1 cut(s) 212
TscAI CASTG 2 cut(s) 299, 756
TseFI GTSAC 1 cut(s) 403
TseI GCWGC 1 cut(s) 339
Tsp45I GTSAC 1 cut(s) 403
TspDTI ATGAA 2 cut(s) 143, 722
TspGWI ACGGA 1 cut(s) 784
TspRI CASTG 2 cut(s) 299, 756
VneI GTGCAC 1 cut(s) 516
VpaK11BI GGWCC 2 cut(s) 487, 695
XapI RAATTY 2 cut(s) 165, 635
XbaI TCTAGA 1 cut(s) 347
XceI RCATGY 3 cut(s) 330, 339, 516
XcmI CCANNNNNNNNNTGG 1 cut(s) 154
XmaJI CCTAGG 1 cut(s) 574
XspI CTAG 5 cut(s) 300, 348, 359, 575, 608
ZrmI AGTACT 2 cut(s) 251, 776
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.