Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0012777

Physical Location & Seq

Genomic Coordinates Reverse (-)

30004855 .. 30005538

Gene Structure 684 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1223300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 684 bp

ATGGCTTCCCAACGTGACCAAACTTTACCTAACCACATAGAAATTCCAGTTCATGGAAATATGACTGATCAACCAAGACAATCTCCAATTCCCGGAGGTGACGAAGATGACACCTTCGATTACTCCCAGAGGACACAGTGGCTTCGCGCCACCATGTTGGGAGCCAATGACGGGCTGGTTTCTGTTGCATCGCTCATGATGGGCGTGGGAGCTGTGAAACAAGATGTGAAGACCATGCTAATAGCCGGGTTTGCAGGTCTCGTGGCCGGGGCGTGTAGTATGGCAATAGGAGAGTTTGTCTCTGTGTACACTCAGTACGACATAGAGATATCTCAAATGAAGAGAGAGATGAAAACAAATGGTAGGACAGAAAATGTAGAGGAGGCAAAGAAGAAGATGCTGCCGAATCCTGCGCAAGCCGCCTTGGCGTCAGCCATTGCGTTTTCGATTGGCGCGGTGGTGCCTCTGTTGGGAGCTGCATTTATAAGTGAGCATAAGGTGAGGCTGGCCGTAGTGGCGGCTTTGGTGAGCGTTGCGCTGGTTGTGTTTGGAGGGGTAGCAGCGAGGCTGGGGGGGTCATCGGTGGTGAAGTCTTGTGCAAGAGTACTTGTTGGAGGATGGATGGCAATGACTATAACTTTTGCACTCACCAAGTTAATTGGGTCTAGTGGTATGCAGGTGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000041 GO:0000302 GO:0003674 GO:0005215 GO:0005381 GO:0005384 GO:0005575 GO:0006810 GO:0006811 GO:0006812 GO:0006826 GO:0006828 GO:0006873 GO:0006875 GO:0006879 GO:0006880 GO:0006950 GO:0006979 GO:0008150 GO:0008324 GO:0009719 GO:0009723 GO:0009725 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010039 GO:0015075 GO:0015318 GO:0016020 GO:0019725 GO:0022857 GO:0022890 GO:0030001 GO:0030003 GO:0030026 GO:0032870 GO:0033554 GO:0034220 GO:0034599 GO:0034614 GO:0034755 GO:0035690 GO:0042221 GO:0042493 GO:0042592 GO:0046873 GO:0046915 GO:0046916 GO:0048878 GO:0050801 GO:0050896 GO:0051179 GO:0051234 GO:0051235 GO:0051238 GO:0051641 GO:0051651 GO:0051716 GO:0055065 GO:0055071 GO:0055072 GO:0055076 GO:0055080 GO:0055082 GO:0055085 GO:0065007 GO:0065008 GO:0070887 GO:0071241 GO:0071248 GO:0071281 GO:0071310 GO:0071369 GO:0071421 GO:0071495 GO:0071731 GO:0071732 GO:0097366 GO:0097577 GO:0098655 GO:0098660 GO:0098662 GO:0098771 GO:1901698 GO:1901699 GO:1901700 GO:1901701 GO:1902170

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

VIT1

Protein Analysis

228

Amino Acids

23.87

Weight (kDa)

7.78

Isoelectric Point (pI)

43.44

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
VIT1	PF01988	48 - 126	3.4e-23	VIT family
VIT1	PF01988	131 - 217	5.8e-17	VIT family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0012777)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G25190
fragaria_vesca	FvH4_6g05360 FvH4_6g06150
malus_domestica	MD04G1208900.v1.1 MD12G1223300.v1.1 MD12G1223400.v1.1
prunus_persica	Prupe.6G329900_v2.0.a1
rosa_chinensis	RchiOBHm_Chr3g0454131
rosa_laevigata	RLG00000025480
rosa_multiflora	Rmu_co8428255.1_g000001 Rmu_sc0018584.1_g000003
rosa_roxburghii	Rroxscaffold_165G00436910
rosa_rugosa	Rorug02G0653500
rosa_samantha	Rh3AG056300 Rh3BG057700 Rh3CG057000 Rh3DG058200
rosa_wichuraiana	Rw3G004310

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	485
AarI	CACCTGC	1 cut(s)	667
Acc16I	TGCGCA	1 cut(s)	414
Acc36I	ACCTGC	2 cut(s)	245, 667
AccB1I	GGYRCC	1 cut(s)	460
AccB7I	CCANNNNNTGG	1 cut(s)	53
AccII	CGCG	2 cut(s)	147, 455
AciI	CCGC	3 cut(s)	420, 455, 518
AcoI	YGGCCR	2 cut(s)	264, 507
AcsI	RAATTY	1 cut(s)	42
AcyI	GRCGYC	1 cut(s)	428
AfaI	GTAC	3 cut(s)	308, 317, 606
AfiI	CCNNNNNNNGG	4 cut(s)	53, 92, 171, 470
AluBI	AGCT	2 cut(s)	212, 476
AluI	AGCT	2 cut(s)	212, 476
Alw26I	GTCTC	2 cut(s)	263, 304
AoxI	GGCC	2 cut(s)	264, 507
ApeKI	GCWGC	3 cut(s)	400, 476, 560
ApoI	RAATTY	1 cut(s)	42
AspLEI	GCGC	4 cut(s)	149, 415, 455, 538
AsuC2I	CCSGG	3 cut(s)	93, 247, 268
AsuHPI	GGTGA	5 cut(s)	110, 511, 538, 598, 640
BanI	GGYRCC	1 cut(s)	460
BauI	CACGAG	1 cut(s)	260
BbsI	GAAGAC	1 cut(s)	236
BbvI	GCAGC	3 cut(s)	387, 463, 572
BccI	CCATC	3 cut(s)	193, 612, 616
BceAI	ACGGC	1 cut(s)	494
BclI	TGATCA	1 cut(s)	67
BcnI	CCSGG	3 cut(s)	93, 247, 268
BcoDI	GTCTC	2 cut(s)	263, 304
BfaI	CTAG	1 cut(s)	666
BfuAI	ACCTGC	2 cut(s)	245, 667
BglI	GCCNNNNNGGC	2 cut(s)	425, 515
BisI	GCNGC	5 cut(s)	401, 420, 477, 519, 561
BlsI	GCNGC	5 cut(s)	402, 421, 478, 520, 562
BmcAI	AGTACT	1 cut(s)	606
Bme1390I	CCNGG	3 cut(s)	93, 247, 268
BmiI	GGNNCC	2 cut(s)	163, 462
BmrFI	CCNGG	3 cut(s)	93, 247, 268
BmsI	GCATC	2 cut(s)	197, 387
BpiI	GAAGAC	1 cut(s)	236
BplI	GAGNNNNNCTC	2 cut(s)	284, 316
BpuMI	CCSGG	3 cut(s)	93, 247, 268
BsaHI	GRCGYC	1 cut(s)	428
BsaI	GGTCTC	1 cut(s)	263
BsaJI	CCNNGG	2 cut(s)	267, 423
BsaXI	ACNNNNNCTCC	2 cut(s)	282, 312
Bsc4I	CCNNNNNNNGG	4 cut(s)	53, 92, 171, 470
Bse1I	ACTGG	1 cut(s)	47
Bse3DI	GCAATG	2 cut(s)	435, 633
BseDI	CCNNGG	2 cut(s)	267, 423
BseGI	GGATG	2 cut(s)	623, 627
BseLI	CCNNNNNNNGG	4 cut(s)	53, 92, 171, 470
BseMI	GCAATG	2 cut(s)	435, 633
BseMII	CTCAG	1 cut(s)	326
BseNI	ACTGG	1 cut(s)	47
BseRI	GAGGAG	1 cut(s)	395
BseXI	GCAGC	3 cut(s)	387, 463, 572
BseYI	CCCAGC	1 cut(s)	568
Bsh1236I	CGCG	2 cut(s)	147, 455
BshFI	GGCC	2 cut(s)	266, 509
BshNI	GGYRCC	1 cut(s)	460
BsiSI	CCGG	3 cut(s)	93, 246, 267
BslI	CCNNNNNNNGG	4 cut(s)	53, 92, 171, 470
BsmAI	GTCTC	2 cut(s)	263, 304
BsnI	GGCC	2 cut(s)	266, 509
Bso31I	GGTCTC	1 cut(s)	263
Bsp1407I	TGTACA	1 cut(s)	306
Bsp143I	GATC	1 cut(s)	67
BspACI	CCGC	3 cut(s)	420, 455, 518
BspANI	GGCC	2 cut(s)	266, 509
BspCNI	CTCAG	1 cut(s)	325
BspFNI	CGCG	2 cut(s)	147, 455
BspHI	TCATGA	1 cut(s)	195
BspLI	GGNNCC	2 cut(s)	163, 462
BspMI	ACCTGC	2 cut(s)	245, 667
BspT107I	GGYRCC	1 cut(s)	460
BspTNI	GGTCTC	1 cut(s)	263
BsrDI	GCAATG	2 cut(s)	435, 633
BsrGI	TGTACA	1 cut(s)	306
BsrI	ACTGG	1 cut(s)	47
BssECI	CCNNGG	2 cut(s)	267, 423
BssMI	GATC	1 cut(s)	67
BssNI	GRCGYC	1 cut(s)	428
BssSI	CACGAG	1 cut(s)	260
BssT1I	CCWWGG	1 cut(s)	423
Bst2BI	CACGAG	1 cut(s)	260
Bst4CI	ACNGT	1 cut(s)	138
Bst6I	CTCTTC	1 cut(s)	335
BstACI	GRCGYC	1 cut(s)	428
BstAUI	TGTACA	1 cut(s)	306
BstC8I	GCNNGC	2 cut(s)	417, 507
BstDEI	CTNAG	1 cut(s)	312
BstF5I	GGATG	2 cut(s)	623, 627
BstFNI	CGCG	2 cut(s)	147, 455
BstHHI	GCGC	4 cut(s)	149, 415, 455, 538
BstKTI	GATC	1 cut(s)	70
BstMAI	GTCTC	2 cut(s)	263, 304
BstMBI	GATC	1 cut(s)	67
BstMWI	GCNNNNNNNGC	4 cut(s)	251, 419, 425, 515
BstSCI	CCNGG	3 cut(s)	91, 245, 266
BstUI	CGCG	2 cut(s)	147, 455
BstV1I	GCAGC	3 cut(s)	387, 463, 572
BstV2I	GAAGAC	1 cut(s)	236
BstXI	CCANNNNNNTGG	1 cut(s)	157
BsuRI	GGCC	2 cut(s)	266, 509
BtgZI	GCGATG	1 cut(s)	174
BtsCI	GGATG	2 cut(s)	623, 627
BtsIMutI	CAGTG	1 cut(s)	143
BveI	ACCTGC	2 cut(s)	245, 667
Cac8I	GCNNGC	2 cut(s)	417, 507
CciI	TCATGA	1 cut(s)	195
CfoI	GCGC	4 cut(s)	149, 415, 455, 538
CseI	GACGC	1 cut(s)	417
Csp6I	GTAC	3 cut(s)	307, 316, 605
CviAII	CATG	4 cut(s)	53, 154, 196, 235
CviQI	GTAC	3 cut(s)	307, 316, 605
DdeI	CTNAG	1 cut(s)	312
DpnI	GATC	1 cut(s)	69
DpnII	GATC	1 cut(s)	67
EaeI	YGGCCR	2 cut(s)	264, 507
Eam1104I	CTCTTC	1 cut(s)	335
EarI	CTCTTC	1 cut(s)	335
Eco130I	CCWWGG	1 cut(s)	423
Eco31I	GGTCTC	1 cut(s)	263
Eco32I	GATATC	1 cut(s)	330
EcoRV	GATATC	1 cut(s)	330
EcoT14I	CCWWGG	1 cut(s)	423
ErhI	CCWWGG	1 cut(s)	423
FaeI	CATG	4 cut(s)	56, 157, 199, 238
FatI	CATG	4 cut(s)	52, 153, 195, 234
FbaI	TGATCA	1 cut(s)	67
Fnu4HI	GCNGC	5 cut(s)	401, 420, 477, 519, 561
FokI	GGATG	2 cut(s)	630, 634
Fsp4HI	GCNGC	5 cut(s)	401, 420, 477, 519, 561
FspBI	CTAG	1 cut(s)	666
FspI	TGCGCA	1 cut(s)	414
GlaI	GCGC	4 cut(s)	148, 414, 454, 537
GluI	GCNGC	5 cut(s)	401, 420, 477, 519, 561
GsaI	CCCAGC	1 cut(s)	572
HaeIII	GGCC	2 cut(s)	266, 509
HapII	CCGG	3 cut(s)	93, 246, 267
HgaI	GACGC	1 cut(s)	417
HhaI	GCGC	4 cut(s)	149, 415, 455, 538
Hin1I	GRCGYC	1 cut(s)	428
Hin1II	CATG	4 cut(s)	56, 157, 199, 238
Hin6I	GCGC	4 cut(s)	147, 413, 453, 536
HinP1I	GCGC	4 cut(s)	147, 413, 453, 536
HinfI	GANTC	1 cut(s)	406
HpaII	CCGG	3 cut(s)	93, 246, 267
HphI	GGTGA	5 cut(s)	110, 511, 538, 598, 640
Hpy166II	GTNNAC	2 cut(s)	307, 309
Hpy188III	TCNNGA	1 cut(s)	196
Hpy8I	GTNNAC	2 cut(s)	307, 309
HpyAV	CCTTC	1 cut(s)	124
HpyCH4III	ACNGT	1 cut(s)	138
HpyCH4IV	ACGT	1 cut(s)	13
HpyCH4V	TGCA	6 cut(s)	188, 254, 479, 599, 644, 676
HpyF10VI	GCNNNNNNNGC	4 cut(s)	251, 419, 425, 515
HpyF3I	CTNAG	1 cut(s)	312
HpySE526I	ACGT	1 cut(s)	13
Hsp92I	GRCGYC	1 cut(s)	428
Hsp92II	CATG	4 cut(s)	56, 157, 199, 238
HspAI	GCGC	4 cut(s)	147, 413, 453, 536
Ksp22I	TGATCA	1 cut(s)	67
Kzo9I	GATC	1 cut(s)	67
LmnI	GCTCC	3 cut(s)	161, 209, 473
Lsp1109I	GCAGC	3 cut(s)	387, 463, 572
LweI	GCATC	2 cut(s)	197, 387
MaeI	CTAG	1 cut(s)	666
MaeII	ACGT	1 cut(s)	13
MaeIII	GTNAC	2 cut(s)	14, 98
MalI	GATC	1 cut(s)	69
MboI	GATC	1 cut(s)	67
MboII	GAAGA	5 cut(s)	116, 241, 352, 403, 406
MluCI	AATT	3 cut(s)	42, 87, 657
MmeI	TCCRAC	1 cut(s)	592
MnlI	CCTC	9 cut(s)	89, 123, 373, 376, 474, 495, 545, 558, 608
MseI	TTAA	1 cut(s)	656
MspI	CCGG	3 cut(s)	93, 246, 267
MspR9I	CCNGG	3 cut(s)	93, 247, 268
MvnI	CGCG	2 cut(s)	147, 455
MwoI	GCNNNNNNNGC	4 cut(s)	251, 419, 425, 515
NciI	CCSGG	3 cut(s)	93, 247, 268
NdeII	GATC	1 cut(s)	67
NlaIII	CATG	4 cut(s)	56, 157, 199, 238
NlaIV	GGNNCC	2 cut(s)	163, 462
NmuCI	GTSAC	2 cut(s)	14, 98
NsbI	TGCGCA	1 cut(s)	414
PagI	TCATGA	1 cut(s)	195
PaqCI	CACCTGC	1 cut(s)	667
PfeI	GAWTC	1 cut(s)	406
PflMI	CCANNNNNTGG	1 cut(s)	53
PfoI	TCCNGGA	1 cut(s)	91
PkrI	GCNGC	5 cut(s)	402, 421, 478, 520, 562
PsiI	TTATAA	1 cut(s)	485
PspFI	CCCAGC	1 cut(s)	568
PspN4I	GGNNCC	2 cut(s)	163, 462
RsaI	GTAC	3 cut(s)	308, 317, 606
RsaNI	GTAC	3 cut(s)	307, 316, 605
SaqAI	TTAA	1 cut(s)	656
SatI	GCNGC	5 cut(s)	401, 420, 477, 519, 561
Sau3AI	GATC	1 cut(s)	67
ScaI	AGTACT	1 cut(s)	606
ScrFI	CCNGG	3 cut(s)	93, 247, 268
SetI	ASST	9 cut(s)	16, 31, 100, 116, 214, 259, 478, 501, 681
SfaNI	GCATC	2 cut(s)	197, 387
Sse9I	AATT	3 cut(s)	42, 87, 657
SsiI	CCGC	3 cut(s)	420, 455, 518
SspMI	CTAG	1 cut(s)	666
StyD4I	CCNGG	3 cut(s)	91, 245, 266
StyI	CCWWGG	1 cut(s)	423
TaaI	ACNGT	1 cut(s)	138
TaiI	ACGT	1 cut(s)	16
TaqI	TCGA	2 cut(s)	117, 446
TasI	AATT	3 cut(s)	42, 87, 657
TatI	WGTACW	2 cut(s)	306, 604
TauI	GCSGC	2 cut(s)	422, 521
TfiI	GAWTC	1 cut(s)	406
Tru1I	TTAA	1 cut(s)	656
Tru9I	TTAA	1 cut(s)	656
TscAI	CASTG	1 cut(s)	143
TseFI	GTSAC	2 cut(s)	14, 98
TseI	GCWGC	3 cut(s)	400, 476, 560
Tsp45I	GTSAC	2 cut(s)	14, 98
TspDTI	ATGAA	3 cut(s)	41, 353, 365
TspRI	CASTG	1 cut(s)	143
Van91I	CCANNNNNTGG	1 cut(s)	53
XapI	RAATTY	1 cut(s)	42
XcmI	CCANNNNNNNNNTGG	1 cut(s)	172
XspI	CTAG	1 cut(s)	666
ZrmI	AGTACT	1 cut(s)	606

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.