MD12G1245600.v1.1

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0018855
Physical Location & Seq
Reverse (-)
31598295 .. 31600771
2477 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1245600.v1.1.491

Sequence Viewer

Length: 2457 bp
ATGAAAACATTGAGAAAAAGATGCAATTCCAAAGCCTACACATCCATGGACCATACAATGGTCACCACCAAACCTCATCTCCTTCCACCTTCCCTCCTTCCTCCCTCCAAATTCAAATTCCTCCAAACCTCTCCAAACAATCACACCCCACACCCAAACACCCACCCATCTACCCACCAACCAACCACTTCTTTTACCCCCCATGGACACCCCAACCTTGACCACACCCAGCAAATCCATGCCCACATGATCAAAACCCAATTTGATCACACCCAGCAAACCCCTCTCACGCACCTCCAAACCCAGCTAAGCCCTGCAGCTCAGCACAACTTCTTGATAACTTCATACATTAAGAACAACTGCCCGGCAATTGCACTCGAAATATATGCCCAAATGCGAAGAATGGACACTCAAGTCGACAGCTTCACCATTCCATCAGTCCTCAAAGCTTGCGGGCAATCGTCTTTCGCGATTTTGGGAAAGGAGACGCACGGCTTCGCGCTGAAGAGCGGGTTGGATGGTGATGTTTTCGTGCGCAACGCGTTGATCCAAATGTATAGCGAAAGTGGGAATGTGGTGCTAGCTCGCTTGCTGTTCGATAAAATGACTGACAGAGATGTTGTTTCTTGGAGTACAATGATTAGGAGCTGTGTTAGGAACAGATTGTTTGGTGAGGCATTGGATCTTATAAGGGAAATGCATCGTGTTCGAGTGAAGCCTAGTGAGATTGCCATGATTAGCATGGTTAATCTCTTTGCGGGGGTTGCTGATATCGAAATGGGGAAAGCGATGCATGTTCATGTTGTGAGGAATGGCAAGCATGAGAAATTCGGAGTTCCTGTTACTACTGCTATGATCGACATGTATGTCAAGTGTGGAAATGTAACTTATGCAAGGAGGCTCTTCGACGGGTTAGGACAGAAAAATATTGTTTCGTGGACTGCAATGATTGCGGGGTACATTCATTGCAGAGATTTACACGAGGGAGCAAAACTTTTCAACAGAATGCTGGCGGAAGGAAGTTTTCCGAATGAGATTACGATGCTGAGTTTGATTATCGAGTCTGGGTCTGTTGGCGCTATGGAATTAGGCAAGTGGCTGCACGCCTACATTCTGAGACATGGGTTTGTCATGTCTTTGCCTTTGGCCACTGCTTTGGTCGACATGTATGGAAAATGCGGAGAAACCAGATATGCAAGGGCTGTTTTCGATAGTGTGGATTACAAAGATGTTATGATTTGGAGTGCCTTGATCTCAGCTTACGCGCATGCCAATTGCACTAATGAGGCTTCACAGCTCTTTGCTCGGATGAAGAATAGCGGAGCAAGGCCAAATCAAGTCACAATGGTTAGCCTGATTTCGTTATGTGCAGAAGTTGGAGCCCTTGACCTGGGCAAGTGGGTTCATTCGTATATAAACCAGCAACGGGTGGAAGTTGATGTCATTTTACGAACAGCTCTAGTTGACATGTATGCGAAGTGTGGAGAAATCGATATGGCTCTCAGGTTGTTTGATGAAGCCACAAATCGAGACATTTGCATGTGGAATGCCATGATGACAGGATTTTCTATGCATGGCTGTGGAAAACAAGCTCTGGAACTCTTTGAACAAATGAACAGAGAAGCTGTGGAGCCTAACGACATCACATTTATCGGAGTTCTCCATGCTTGCAGTCACGCTGGATTGGTGGCAGAAGGAGAGAATTTTTTTGAGAAAATGGTTCGTGTCCATGGATTGGCTCCGAAGGTTGAGCACTACGGATGCATGGTGGATTTGTTAGGTCGAGCTGGGAAACTCCACGAGGCTCATGAACTGATCAAAAGCATGCCCATACAGCCTAACACAATTGTATGGGGTGCCCTGCTCGCTGCGTGCAAAATCCATAAAAATCCCGACTTGGCTGAAGTTGCGTCAAGGCAGCTTCTCCAGTTGGAACCTCGAAATTGTGGATACAACATTCTGATGTCGAACATCTATGCAGCATCAAACAGATGGAATGATGTTGCCGGGGTGAGAAAAGCCATGAAAGACAGAGGAACCAAGAAAGAACCCGGTCTCAGCTCGATCGAAGTAAATGGCTCGGTCCATGACTTTGTAATGGGGGATAGAGCGCACCCTCAAGCCGGAAAAATTTACGAAATGCTGGCTGAGATGATCAAGAAGCTGAAAGAGGCAGGTTACGCTCCTAACACGTCGGTCGTGTTGCAGAACATAGATGAGGAAGAGAAAGAAACTGCGGTTAACTATCACAGCGAGAAACTGGCCATGGCATTCGGTTTGATCGCTGCTGCTGCAGGTACCCCGATTCGGGTTGTGAAGAACCTCCGAGTCTGTGACGATTGCCACACAGCAACAAAGCTTTTATCAAAAATTTACGGGAGGGTAATTATAGTAAGGGACCGGAATCGCTTTCACCACTTCAGAGAAGGATCCTGTTCTTGTGGTGATTATTGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DYW_deaminase PPR PPR_2

Protein Analysis

819

Amino Acids

91.39

Weight (kDa)

8.59

Isoelectric Point (pI)

36.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 205 - 252 1.3e-06 PPR repeat family
PPR PF01535 208 - 235 3.7e-06 PPR repeat
PPR_2 PF13041 308 - 352 1.2e-07 PPR repeat family
PPR_2 PF13041 409 - 457 3.8e-09 PPR repeat family
PPR_2 PF13041 511 - 558 2e-10 PPR repeat family
E_motif PF20431 628 - 690 4.6e-18 E motif
DYW_deaminase PF14432 726 - 818 1.6e-40 DYW family of nucleic acid deaminases
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0018855)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g02300
malus_domestica MD12G1245600.v1.1
prunus_persica Prupe.6G348400_v2.0.a1
rosa_chinensis RchiOBHm_Chr3g0450051
rosa_laevigata RLG00000025761
rosa_multiflora Rmu_sc0002646.1_g000023
rosa_rugosa Rorug02G0625100
rosa_samantha Rh3DG024800
rosa_wichuraiana Rw3G001930

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 689
AasI GACNNNNNNGTC 2 cut(s) 413, 866
Acc16I TGCGCA 1 cut(s) 536
Acc36I ACCTGC 2 cut(s) 2165, 2285
Acc65I GGTACC 1 cut(s) 2297
AccB1I GGYRCC 2 cut(s) 1856, 2297
AccB7I CCANNNNNTGG 2 cut(s) 58, 1735
AccBSI CCGCTC 1 cut(s) 510
AccI GTMKAC 2 cut(s) 417, 1161
AccII CGCG 4 cut(s) 470, 500, 542, 1265
AciI CCGC 8 cut(s) 453, 510, 758, 953, 1013, 1179, 1320, 2237
AclWI GGATC 4 cut(s) 541, 690, 2424, 2437
AcoI YGGCCR 2 cut(s) 1146, 2262
AcsI RAATTY 6 cut(s) 110, 116, 827, 1702, 2130, 2370
AcuI CTGAAG 3 cut(s) 524, 1923, 2404
AfaI GTAC 3 cut(s) 634, 959, 2299
AfiI CCNNNNNNNGG 7 cut(s) 58, 1390, 1426, 1735, 2123, 2307, 2308
AflIII ACRYGT 5 cut(s) 540, 861, 1164, 1467, 2190
AgsI TTSAA 3 cut(s) 115, 1000, 1607
AjiI CACGTC 1 cut(s) 2193
AjnI CCWGG 1 cut(s) 1389
AjuI GAANNNNNNNTTGG 4 cut(s) 101, 133, 497, 529
Alw21I GWGCWC 1 cut(s) 1755
Alw26I GTCTC 4 cut(s) 479, 1111, 1524, 2060
AlwI GGATC 4 cut(s) 541, 690, 2424, 2437
AoxI GGCC 3 cut(s) 1146, 1328, 2262
ApeKI GCWGC 8 cut(s) 317, 1099, 1868, 1918, 1979, 2285, 2288, 2291
ApoI RAATTY 6 cut(s) 110, 116, 827, 1702, 2130, 2370
Asp718I GGTACC 1 cut(s) 2297
AspLEI GCGC 5 cut(s) 502, 537, 1079, 1267, 2113
AspS9I GGNCC 3 cut(s) 49, 2083, 2398
AsuC2I CCSGG 3 cut(s) 365, 2008, 2052
AsuHPI GGTGA 7 cut(s) 55, 418, 533, 683, 2023, 2405, 2456
AsuNHI GCTAGC 1 cut(s) 580
AvaII GGWCC 3 cut(s) 49, 2083, 2398
BaeGI GKGCMC 1 cut(s) 1861
BalI TGGCCA 2 cut(s) 1148, 2264
BamHI GGATCC 1 cut(s) 2429
BanI GGYRCC 2 cut(s) 1856, 2297
BanII GRGCYC 1 cut(s) 1384
BauI CACGAG 2 cut(s) 980, 1799
Bbv12I GWGCWC 1 cut(s) 1755
BbvI GCAGC 8 cut(s) 329, 1086, 1855, 1930, 1991, 2272, 2275, 2278
BccI CCATC 4 cut(s) 175, 442, 512, 1986
BceAI ACGGC 1 cut(s) 508
BcgI CGANNNNNNTGC 6 cut(s) 368, 402, 689, 723, 1518, 1552
BciT130I CCWGG 1 cut(s) 1391
BciVI GTATCC 1 cut(s) 1943
BclI TGATCA 4 cut(s) 249, 265, 1815, 2154
BcnI CCSGG 3 cut(s) 365, 2008, 2052
BcoDI GTCTC 4 cut(s) 479, 1111, 1524, 2060
BfaI CTAG 3 cut(s) 581, 720, 1460
BfmI CTRYAG 2 cut(s) 315, 2292
BfoI RGCGCY 1 cut(s) 1080
BfuAI ACCTGC 2 cut(s) 2165, 2285
BfuI GTATCC 1 cut(s) 1943
BisI GCNGC 8 cut(s) 318, 1100, 1869, 1919, 1980, 2286, 2289, 2292
BlpI GCTNAGC 2 cut(s) 308, 321
BlsI GCNGC 8 cut(s) 319, 1101, 1870, 1920, 1981, 2287, 2290, 2293
Bme1390I CCNGG 4 cut(s) 365, 1391, 2008, 2052
Bme18I GGWCC 3 cut(s) 49, 2083, 2398
BmgBI CACGTC 1 cut(s) 2193
BmgT120I GGNCC 3 cut(s) 49, 2083, 2398
BmiI GGNNCC 9 cut(s) 1381, 1632, 1740, 1858, 1935, 2038, 2299, 2399, 2431
BmrFI CCNGG 4 cut(s) 365, 1391, 2008, 2052
BmsI GCATC 6 cut(s) 11, 709, 780, 1032, 1751, 1991
BmtI GCTAGC 1 cut(s) 584
BpmI CTGGAG 1 cut(s) 1910
Bpu1102I GCTNAGC 2 cut(s) 308, 321
BpuEI CTTGAG 2 cut(s) 396, 2103
BpuMI CCSGG 3 cut(s) 365, 2008, 2052
Bsa29I ATCGAT 1 cut(s) 1491
BsaI GGTCTC 1 cut(s) 2060
BsaJI CCNNGG 6 cut(s) 45, 202, 1390, 1729, 2007, 2265
BsaWI WCCGGW 1 cut(s) 2400
BsaXI ACNNNNNCTCC 6 cut(s) 63, 78, 93, 108, 825, 855
Bsc4I CCNNNNNNNGG 7 cut(s) 58, 1390, 1426, 1735, 2123, 2307, 2308
Bse1I ACTGG 2 cut(s) 1927, 2265
Bse3DI GCAATG 2 cut(s) 951, 964
BseBI CCWGG 1 cut(s) 1391
BseCI ATCGAT 1 cut(s) 1491
BseDI CCNNGG 6 cut(s) 45, 202, 1390, 1729, 2007, 2265
BseGI GGATG 4 cut(s) 41, 523, 1314, 1766
BseLI CCNNNNNNNGG 7 cut(s) 58, 1390, 1426, 1735, 2123, 2307, 2308
BseMI GCAATG 2 cut(s) 951, 964
BseMII CTCAG 7 cut(s) 335, 1037, 1106, 1269, 1516, 2071, 2139
BseNI ACTGG 2 cut(s) 1927, 2265
BseSI GKGCMC 1 cut(s) 1861
BseXI GCAGC 8 cut(s) 329, 1086, 1855, 1930, 1991, 2272, 2275, 2278
BseYI CCCAGC 4 cut(s) 228, 273, 303, 1787
BsgI GTGCAG 2 cut(s) 1085, 1389
Bsh1236I CGCG 4 cut(s) 470, 500, 542, 1265
Bsh1285I CGRYCG 2 cut(s) 2067, 2199
BshFI GGCC 3 cut(s) 1148, 1330, 2264
BshNI GGYRCC 2 cut(s) 1856, 2297
BshVI ATCGAT 1 cut(s) 1491
BsiEI CGRYCG 2 cut(s) 2067, 2199
BsiHKAI GWGCWC 1 cut(s) 1755
BsiSI CCGG 5 cut(s) 365, 2007, 2052, 2124, 2401
BslFI GGGAC 1 cut(s) 2411
BslI CCNNNNNNNGG 7 cut(s) 58, 1390, 1426, 1735, 2123, 2307, 2308
BsmAI GTCTC 4 cut(s) 479, 1111, 1524, 2060
BsmBI CGTCTC 1 cut(s) 479
BsmFI GGGAC 1 cut(s) 2411
BsmI GAATGC 3 cut(s) 1011, 1552, 2270
BsnI GGCC 3 cut(s) 1148, 1330, 2264
Bso31I GGTCTC 1 cut(s) 2060
Bsp1286I GDGCHC 3 cut(s) 1384, 1755, 1861
Bsp1720I GCTNAGC 2 cut(s) 308, 321
Bsp19I CCATGG 4 cut(s) 45, 202, 1729, 2265
Bsp68I TCGCGA 1 cut(s) 470
BspACI CCGC 8 cut(s) 453, 510, 758, 953, 1013, 1179, 1320, 2237
BspANI GGCC 3 cut(s) 1148, 1330, 2264
BspCNI CTCAG 7 cut(s) 334, 1038, 1107, 1268, 1515, 2070, 2140
BspDI ATCGAT 1 cut(s) 1491
BspFNI CGCG 4 cut(s) 470, 500, 542, 1265
BspHI TCATGA 1 cut(s) 1807
BspLI GGNNCC 9 cut(s) 1381, 1632, 1740, 1858, 1935, 2038, 2299, 2399, 2431
BspMAI CTGCAG 2 cut(s) 319, 2296
BspMI ACCTGC 2 cut(s) 2165, 2285
BspOI GCTAGC 1 cut(s) 584
BspPI GGATC 4 cut(s) 541, 690, 2424, 2437
BspQI GCTCTTC 2 cut(s) 500, 908
BspT107I GGYRCC 2 cut(s) 1856, 2297
BspTNI GGTCTC 1 cut(s) 2060
BsrBI CCGCTC 1 cut(s) 510
BsrDI GCAATG 2 cut(s) 951, 964
BsrI ACTGG 2 cut(s) 1927, 2265
BssECI CCNNGG 6 cut(s) 45, 202, 1390, 1729, 2007, 2265
BssSI CACGAG 2 cut(s) 980, 1799
BssT1I CCWWGG 4 cut(s) 45, 202, 1729, 2265
Bst2BI CACGAG 2 cut(s) 980, 1799
Bst2UI CCWGG 1 cut(s) 1391
Bst6I CTCTTC 3 cut(s) 500, 908, 2217
BstAPI GCANNNNNTGC 1 cut(s) 950
BstDEI CTNAG 8 cut(s) 308, 321, 1046, 1115, 1255, 1502, 2057, 2148
BstDSI CCRYGG 4 cut(s) 45, 202, 1729, 2265
BstEII GGTNACC 1 cut(s) 61
BstF5I GGATG 4 cut(s) 41, 523, 1314, 1766
BstFNI CGCG 4 cut(s) 470, 500, 542, 1265
BstH2I RGCGCY 1 cut(s) 1080
BstHHI GCGC 5 cut(s) 502, 537, 1079, 1267, 2113
BstMAI GTCTC 4 cut(s) 479, 1111, 1524, 2060
BstMCI CGRYCG 2 cut(s) 2067, 2199
BstMWI GCNNNNNNNGC 7 cut(s) 764, 950, 1834, 1865, 1907, 2180, 2291
BstNI CCWGG 1 cut(s) 1391
BstNSI RCATGY 7 cut(s) 797, 865, 1168, 1271, 1471, 1543, 1828
BstPI GGTNACC 1 cut(s) 61
BstSCI CCNGG 4 cut(s) 363, 1389, 2006, 2050
BstSFI CTRYAG 2 cut(s) 315, 2292
BstSLI GKGCMC 1 cut(s) 1861
BstUI CGCG 4 cut(s) 470, 500, 542, 1265
BstV1I GCAGC 8 cut(s) 329, 1086, 1855, 1930, 1991, 2272, 2275, 2278
BstX2I RGATCY 2 cut(s) 682, 2429
BstXI CCANNNNNNTGG 1 cut(s) 1156
BstYI RGATCY 2 cut(s) 682, 2429
Bsu15I ATCGAT 1 cut(s) 1491
BsuI GTATCC 1 cut(s) 1943
BsuRI GGCC 3 cut(s) 1148, 1330, 2264
BsuTUI ATCGAT 1 cut(s) 1491
BtgI CCRYGG 4 cut(s) 45, 202, 1729, 2265
BtgZI GCGATG 1 cut(s) 803
BtrI CACGTC 1 cut(s) 2193
BtsCI GGATG 4 cut(s) 41, 523, 1314, 1766
BtsI GCAGTG 1 cut(s) 1149
BtsIMutI CAGTG 1 cut(s) 1149
BtuMI TCGCGA 1 cut(s) 470
BveI ACCTGC 2 cut(s) 2165, 2285
CciI TCATGA 1 cut(s) 1807
CfoI GCGC 5 cut(s) 502, 537, 1079, 1267, 2113
Cfr13I GGNCC 3 cut(s) 49, 2083, 2398
ClaI ATCGAT 1 cut(s) 1491
CseI GACGC 2 cut(s) 496, 1899
Csp6I GTAC 3 cut(s) 633, 958, 2298
CviQI GTAC 3 cut(s) 633, 958, 2298
DdeI CTNAG 8 cut(s) 308, 321, 1046, 1115, 1255, 1502, 2057, 2148
DrdI GACNNNNNNGTC 2 cut(s) 413, 866
DseDI GACNNNNNNGTC 2 cut(s) 413, 866
EaeI YGGCCR 2 cut(s) 1146, 2262
Eam1104I CTCTTC 3 cut(s) 500, 908, 2217
EarI CTCTTC 3 cut(s) 500, 908, 2217
EciI GGCGGA 1 cut(s) 1028
Eco130I CCWWGG 4 cut(s) 45, 202, 1729, 2265
Eco24I GRGCYC 1 cut(s) 1384
Eco31I GGTCTC 1 cut(s) 2060
Eco32I GATATC 1 cut(s) 772
Eco47I GGWCC 3 cut(s) 49, 2083, 2398
Eco57I CTGAAG 3 cut(s) 524, 1923, 2404
Eco91I GGTNACC 1 cut(s) 61
EcoO65I GGTNACC 1 cut(s) 61
EcoRII CCWGG 1 cut(s) 1389
EcoRV GATATC 1 cut(s) 772
EcoT14I CCWWGG 4 cut(s) 45, 202, 1729, 2265
EcoT22I ATGCAT 4 cut(s) 702, 795, 1575, 1766
EcoT38I GRGCYC 1 cut(s) 1384
ErhI CCWWGG 4 cut(s) 45, 202, 1729, 2265
Esp3I CGTCTC 1 cut(s) 479
FaqI GGGAC 1 cut(s) 2411
FauI CCCGC 4 cut(s) 446, 503, 751, 946
FbaI TGATCA 4 cut(s) 249, 265, 1815, 2154
FblI GTMKAC 2 cut(s) 417, 1161
Fnu4HI GCNGC 8 cut(s) 318, 1100, 1869, 1919, 1980, 2286, 2289, 2292
FokI GGATG 4 cut(s) 28, 530, 1321, 1773
FriOI GRGCYC 1 cut(s) 1384
Fsp4HI GCNGC 8 cut(s) 318, 1100, 1869, 1919, 1980, 2286, 2289, 2292
FspBI CTAG 3 cut(s) 581, 720, 1460
FspI TGCGCA 1 cut(s) 536
GlaI GCGC 5 cut(s) 501, 536, 1078, 1266, 2112
GluI GCNGC 8 cut(s) 318, 1100, 1869, 1919, 1980, 2286, 2289, 2292
GsaI CCCAGC 4 cut(s) 232, 277, 307, 1791
GsuI CTGGAG 1 cut(s) 1910
HaeII RGCGCY 1 cut(s) 1080
HaeIII GGCC 3 cut(s) 1148, 1330, 2264
HapII CCGG 5 cut(s) 365, 2007, 2052, 2124, 2401
HgaI GACGC 2 cut(s) 496, 1899
HhaI GCGC 5 cut(s) 502, 537, 1079, 1267, 2113
Hin6I GCGC 5 cut(s) 500, 535, 1077, 1265, 2111
HinP1I GCGC 5 cut(s) 500, 535, 1077, 1265, 2111
HincII GTYRAC 4 cut(s) 418, 1162, 1465, 2242
HindII GTYRAC 4 cut(s) 418, 1162, 1465, 2242
HindIII AAGCTT 2 cut(s) 447, 2357
HinfI GANTC 4 cut(s) 1061, 2305, 2328, 2404
HpaI GTTAAC 1 cut(s) 2242
HpaII CCGG 5 cut(s) 365, 2007, 2052, 2124, 2401
HphI GGTGA 7 cut(s) 55, 418, 533, 683, 2023, 2405, 2456
Hpy166II GTNNAC 5 cut(s) 418, 939, 1162, 1465, 2242
Hpy188I TCNGA 9 cut(s) 833, 1029, 1116, 1308, 1655, 1743, 1962, 2327, 2423
Hpy188III TCNNGA 7 cut(s) 334, 469, 1529, 1595, 1808, 1892, 2158
Hpy8I GTNNAC 5 cut(s) 418, 939, 1162, 1465, 2242
Hpy99I CGWCG 2 cut(s) 911, 2197
HpyAV CCTTC 7 cut(s) 92, 99, 107, 1010, 1688, 1738, 2420
HpyCH4IV ACGT 1 cut(s) 2192
HpyF10VI GCNNNNNNNGC 7 cut(s) 764, 950, 1834, 1865, 1907, 2180, 2291
HpyF3I CTNAG 8 cut(s) 308, 321, 1046, 1115, 1255, 1502, 2057, 2148
HpySE526I ACGT 1 cut(s) 2192
HspAI GCGC 5 cut(s) 500, 535, 1077, 1265, 2111
KpnI GGTACC 1 cut(s) 2301
Ksp22I TGATCA 4 cut(s) 249, 265, 1815, 2154
KspAI GTTAAC 1 cut(s) 2242
LguI GCTCTTC 2 cut(s) 500, 908
LmnI GCTCC 7 cut(s) 645, 986, 1322, 1379, 1630, 1744, 2188
Lsp1109I GCAGC 8 cut(s) 329, 1086, 1855, 1930, 1991, 2272, 2275, 2278
LweI GCATC 6 cut(s) 11, 709, 780, 1032, 1751, 1991
MaeI CTAG 3 cut(s) 581, 720, 1460
MaeII ACGT 1 cut(s) 2192
MaeIII GTNAC 7 cut(s) 61, 841, 883, 1339, 1673, 2177, 2333
MbiI CCGCTC 1 cut(s) 510
MboII GAAGA 6 cut(s) 411, 517, 895, 1324, 2234, 2329
MfeI CAATTG 3 cut(s) 369, 1273, 1845
MflI RGATCY 2 cut(s) 682, 2429
MhlI GDGCHC 3 cut(s) 1384, 1755, 1861
MlsI TGGCCA 2 cut(s) 1148, 2264
MluI ACGCGT 1 cut(s) 540
MluNI TGGCCA 2 cut(s) 1148, 2264
MlyI GAGTC 2 cut(s) 1070, 2337
MmeI TCCRAC 3 cut(s) 495, 1357, 1911
Mox20I TGGCCA 2 cut(s) 1148, 2264
Mph1103I ATGCAT 4 cut(s) 702, 795, 1575, 1766
MscI TGGCCA 2 cut(s) 1148, 2264
MseI TTAA 3 cut(s) 351, 747, 2241
MslI CAYNNNNRTG 5 cut(s) 44, 798, 1538, 1578, 1961
Msp20I TGGCCA 2 cut(s) 1148, 2264
MspI CCGG 5 cut(s) 365, 2007, 2052, 2124, 2401
MspR9I CCNGG 4 cut(s) 365, 1391, 2008, 2052
MunI CAATTG 3 cut(s) 369, 1273, 1845
Mva1269I GAATGC 3 cut(s) 1011, 1552, 2270
MvaI CCWGG 1 cut(s) 1391
MvnI CGCG 4 cut(s) 470, 500, 542, 1265
MwoI GCNNNNNNNGC 7 cut(s) 764, 950, 1834, 1865, 1907, 2180, 2291
NciI CCSGG 3 cut(s) 365, 2008, 2052
NcoI CCATGG 4 cut(s) 45, 202, 1729, 2265
NheI GCTAGC 1 cut(s) 580
NlaIV GGNNCC 9 cut(s) 1381, 1632, 1740, 1858, 1935, 2038, 2299, 2399, 2431
NmuCI GTSAC 4 cut(s) 61, 1339, 1673, 2333
NruI TCGCGA 1 cut(s) 470
NsbI TGCGCA 1 cut(s) 536
NsiI ATGCAT 4 cut(s) 702, 795, 1575, 1766
NspI RCATGY 7 cut(s) 797, 865, 1168, 1271, 1471, 1543, 1828
PaeI GCATGC 2 cut(s) 1271, 1828
PagI TCATGA 1 cut(s) 1807
PciI ACATGT 3 cut(s) 861, 1164, 1467
PciSI GCTCTTC 2 cut(s) 500, 908
PcsI WCGNNNNNNNCGW 1 cut(s) 467
PctI GAATGC 3 cut(s) 1011, 1552, 2270
PfeI GAWTC 2 cut(s) 2305, 2404
PflMI CCANNNNNTGG 2 cut(s) 58, 1735
PkrI GCNGC 8 cut(s) 319, 1101, 1870, 1920, 1981, 2287, 2290, 2293
Ple19I CGATCG 1 cut(s) 2067
PleI GAGTC 2 cut(s) 1069, 2336
PpsI GAGTC 2 cut(s) 1069, 2336
PscI ACATGT 3 cut(s) 861, 1164, 1467
PsiI TTATAA 1 cut(s) 689
Psp6I CCWGG 1 cut(s) 1389
PspEI GGTNACC 1 cut(s) 61
PspFI CCCAGC 4 cut(s) 228, 273, 303, 1787
PspGI CCWGG 1 cut(s) 1389
PspN4I GGNNCC 9 cut(s) 1381, 1632, 1740, 1858, 1935, 2038, 2299, 2399, 2431
PspPI GGNCC 3 cut(s) 49, 2083, 2398
PstI CTGCAG 2 cut(s) 319, 2296
PsuI RGATCY 2 cut(s) 682, 2429
PvuI CGATCG 1 cut(s) 2067
RruI TCGCGA 1 cut(s) 470
RsaI GTAC 3 cut(s) 634, 959, 2299
RsaNI GTAC 3 cut(s) 633, 958, 2298
RseI CAYNNNNRTG 5 cut(s) 44, 798, 1538, 1578, 1961
SalI GTCGAC 2 cut(s) 416, 1160
SapI GCTCTTC 2 cut(s) 500, 908
SaqAI TTAA 3 cut(s) 351, 747, 2241
SatI GCNGC 8 cut(s) 318, 1100, 1869, 1919, 1980, 2286, 2289, 2292
Sau96I GGNCC 3 cut(s) 49, 2083, 2398
SchI GAGTC 2 cut(s) 1070, 2337
ScrFI CCNGG 4 cut(s) 365, 1391, 2008, 2052
SduI GDGCHC 3 cut(s) 1384, 1755, 1861
SfaNI GCATC 6 cut(s) 11, 709, 780, 1032, 1751, 1991
SfcI CTRYAG 2 cut(s) 315, 2292
SinI GGWCC 3 cut(s) 49, 2083, 2398
SmiMI CAYNNNNRTG 5 cut(s) 44, 798, 1538, 1578, 1961
SmlI CTYRAG 2 cut(s) 411, 2118
SmoI CTYRAG 2 cut(s) 411, 2118
SphI GCATGC 2 cut(s) 1271, 1828
SsiI CCGC 8 cut(s) 453, 510, 758, 953, 1013, 1179, 1320, 2237
SspI AATATT 1 cut(s) 928
SspMI CTAG 3 cut(s) 581, 720, 1460
StyD4I CCNGG 4 cut(s) 363, 1389, 2006, 2050
StyI CCWWGG 4 cut(s) 45, 202, 1729, 2265
TaiI ACGT 1 cut(s) 2195
TaqII GACCGA 2 cut(s) 2071, 2185
TatI WGTACW 1 cut(s) 632
TfiI GAWTC 2 cut(s) 2305, 2404
Tru1I TTAA 3 cut(s) 351, 747, 2241
Tru9I TTAA 3 cut(s) 351, 747, 2241
TscAI CASTG 1 cut(s) 1156
TseFI GTSAC 4 cut(s) 61, 1339, 1673, 2333
TseI GCWGC 8 cut(s) 317, 1099, 1868, 1918, 1979, 2285, 2288, 2291
Tsp45I GTSAC 4 cut(s) 61, 1339, 1673, 2333
TspGWI ACGGA 1 cut(s) 1773
TspRI CASTG 1 cut(s) 1156
Van91I CCANNNNNTGG 2 cut(s) 58, 1735
VpaK11BI GGWCC 3 cut(s) 49, 2083, 2398
XapI RAATTY 6 cut(s) 110, 116, 827, 1702, 2130, 2370
XceI RCATGY 7 cut(s) 797, 865, 1168, 1271, 1471, 1543, 1828
XcmI CCANNNNNNNNNTGG 1 cut(s) 739
XmiI GTMKAC 2 cut(s) 417, 1161
XspI CTAG 3 cut(s) 581, 720, 1460
Zsp2I ATGCAT 4 cut(s) 702, 795, 1575, 1766
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.