Rw3G001930

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0018855
Physical Location & Seq
Forward (+)
1702924 .. 1705416
2493 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G001930.1

Sequence Viewer

Length: 2457 bp
ATGGGTATATCTAACTGTAGTCCCACAGATACTTCAATGGACCAAATTATGGTCACAACCAGACCTCATCTCCTATCATCTTCCCTCCTTCCTCTCACAATTCAAAATCTCCAACAACCATTTCTTCCCTCCAAATCCAAACTCAACCAAACCCCAAACCCAAACTCAGACCCATCTAAACACCAACCAAGCATTACTTATTCTTTCACTCCCAGCCTTGAACAAACCCAGCAAATCCATGCCCACATGATCAAAACCCAATTTGACCACACCCTTCAAATCCCACTCAATTCCCTCCAAACCCACATGAGCCCTTCAGCTCAACACAACTTCCTAATAACATCTTACATCAAGAACAACTACCCCAAAATTGCACTCAAATTATACACCCAAATGAGAAGAATGGGCAGCCAAGTTGACAACTTTACTATCCCATCAGTCCTCAAGGCCTGTGGTCAAGCTTCTATGGAACTATTAGGAAGAGAGACCCATGGTTTCGCACTAAAGGGCGGTTTGGATGGTGACGCTTTCGTGTGCAATGCATTGATCCAAATGTATAGTGAATGTGGGAGCTTGGTGTTGGCACGCCTAGTATTTGATGAAATGATTGAAAGAGATGTGGTTTCTTGGAGTACCATGATTAATAGCTATGTGAGGAACAGAGTGTTTGGTGAGGCATTGGAACTTATAAGGGAGATGCATTGCTTGCAAGTGAAGCCTAGTGAGGTTGCTATGATGAGCATGGTTAGTCTATTTGCTGGCATTGCTGATGTGAAAATGGGGAAAGCAATGCATGCTTATGTTGCGAGGAATAGCAGTAATGAGAGAATGGTGGTTCATGTTACTACTGCTTTAGTAGATATGTATGTCAAGTGTGGGAATTTGGCTTATGGAAGGAGGCTGTTTGATGGGTTGGCTCAGAAAAGTGTTGTTTCCTGGACTGCGATGATCGCGGGGTGCATTCGTTGCAATGAGGTAGAAGAGGGGGTGAAACTTTTTAAGAGAATGCTGGAGGAGAGGAAGTTTCCTAATGAGATTACAATGTTGAGTTTGGTTATCGAGTCGGGGTCTGTTGGAGCTCTGGAATTGGGGAAGTGGCTGCATGCCTATGTTTTGAGAAATGGGTTTATCATGTCTTTGGCTTTGGCCACTGCTTTGGTTGATATGTATGGGAAGTGTGGTGAAGCCGAATATGCAAGGGCTGTGTTTGACAGCATGGAGAAGAAAGATGTCATGATTTGGAGTGCTATGATCTCGGCTTATGCACGCTCCAATTGTACTAATCAGGCTTCGGAGCTCTTTGCCCGGATGAAGGATAGTGGAATAAGGCCAAATCAAGTTACAATGGTTAGCCTCATTTCACTATGTGCAGAAGTAGGAGCCCTTGATCTGGGAAAATGGGTTCATTCTTACATAAACCAGCAACGGATAGAAGTTGATGTGATACTAAGAACAGCTCTAGTGGACATGTATGCAAAATGTGGGGAGATAGATGCGGCTCTCAGGCTGTTTAGTGAAGCCAGATATCGAGACAGTCGAATGTGGAATGCTACGATCACTGGATTTTCCATGCATGGTTGTGGGAAACAAGCTTTAGAGCTCTTTGAAGAAATGCAGAGAGCAGGTGTCGAGCCTAACGACATCACATTTATAGGGCTACTTCATGCTTGTAGCCATGCTGGGTTGGTGGCAGATGGAAAGAAGGTTTTCGAGAAAATGGTACTTGACTTTGGCTTGGCTCCAAAGGTTGAGCACTATGGATGCATGGTGGATTTGCTAGGCCGAGCTGGGAAGCTCGATGAGGCTCACGAGTTGATCAAAAGCATGCCCGTGGAGCCTAACGCAATTGTTTGGGGCTCCCTGCTTGCTGCCTGCAAAATTCATAAGAGTCCCAACTTGGCTGAAGTGGCAGCAAGACAACTGCTTGAGTTGGAACCTCCAAATTGCGGTTATAACGTTCTGATGTCAAACATCTATGCAGCATCAAACAGATGGATTGATGTTGCTGGGATTAGAAAAGCTATGAAAGATAAAGGAACCAAGAAAGAACCAGGTCTCAGCTCAATTGAAGTAAATGGTGCAGTTCATGACTTCATGATGGGTGATAAAACACACCCGCAAACAAGAAAAATTTATGAAATGCTGGCTGAGATGATCAAGAAGCTAAAGGAGGCTGGATACACTCCTAATACATCTGTGGTTTTGCAGAACATAGATGAGGAAGAGAAAGAAACTGCGGTAAACTATCATAGTGAGAAACTGGCCATGGCATTTGGTCTAATCAGTACTGCTGCAGGTACCCCAATTCGGATTGTAAAAAATCTCCGAGTTTGTGATGACTGTCACACAGCAACAAAGTTGTTATCAAAGATTTATGGCAGAGTAATGATAGTAAGGGACCGTAATCGCTTTCACCACTTCATAGAAGGATCTTGTTCTTGTGGTGACTATTGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DYW_deaminase PPR PPR_2

Protein Analysis

818

Amino Acids

91.14

Weight (kDa)

7.77

Isoelectric Point (pI)

37.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 110 - 151 5e-06 PPR repeat family
PPR_2 PF13041 205 - 250 5.6e-07 PPR repeat family
PPR_2 PF13041 309 - 350 1.7e-07 PPR repeat family
PPR PF01535 311 - 339 2.3e-06 PPR repeat
PPR_2 PF13041 409 - 457 3.4e-10 PPR repeat family
PPR_2 PF13041 511 - 558 1.6e-10 PPR repeat family
E_motif PF20431 632 - 690 3e-18 E motif
Eplus_motif PF20430 695 - 722 6.6e-06 E+ motif
DYW_deaminase PF14432 726 - 818 5.7e-40 DYW family of nucleic acid deaminases
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0018855)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g02300
malus_domestica MD12G1245600.v1.1
prunus_persica Prupe.6G348400_v2.0.a1
rosa_chinensis RchiOBHm_Chr3g0450051
rosa_laevigata RLG00000025761
rosa_multiflora Rmu_sc0002646.1_g000023
rosa_rugosa Rorug02G0625100
rosa_samantha Rh3DG024800
rosa_wichuraiana Rw3G001930

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 689, 1953
AarI CACCTGC 1 cut(s) 1613
Acc36I ACCTGC 2 cut(s) 1613, 2285
Acc65I GGTACC 1 cut(s) 2297
AccB1I GGYRCC 1 cut(s) 2297
AccB7I CCANNNNNTGG 1 cut(s) 49
AccII CGCG 1 cut(s) 953
AciI CCGC 6 cut(s) 510, 953, 1496, 1947, 2117, 2237
AclI AACGTT 1 cut(s) 1956
AclWI GGATC 2 cut(s) 541, 2437
AcoI YGGCCR 2 cut(s) 1146, 2262
AcsI RAATTY 3 cut(s) 880, 1878, 2130
AcuI CTGAAG 2 cut(s) 300, 1923
AdeI CACNNNGTG 1 cut(s) 1367
AfaI GTAC 5 cut(s) 634, 1279, 1722, 2287, 2299
AfiI CCNNNNNNNGG 5 cut(s) 49, 1312, 1390, 1946, 2307
AflIII ACRYGT 1 cut(s) 1467
AgsI TTSAA 7 cut(s) 36, 104, 221, 278, 611, 1607, 2069
AjnI CCWGG 2 cut(s) 935, 2050
AjuI GAANNNNNNNTTGG 2 cut(s) 105, 137
AloI GAACNNNNNNTCC 2 cut(s) 1386, 1418
Alw21I GWGCWC 4 cut(s) 1081, 1299, 1602, 1755
Alw26I GTCTC 3 cut(s) 479, 1524, 2060
AlwI GGATC 2 cut(s) 541, 2437
AoxI GGCC 5 cut(s) 447, 1146, 1328, 1780, 2262
ApeKI GCWGC 6 cut(s) 408, 1099, 1868, 1910, 1979, 2291
ApoI RAATTY 3 cut(s) 880, 1878, 2130
AseI ATTAAT 1 cut(s) 642
Asp700I GAANNNNTTC 1 cut(s) 1706
Asp718I GGTACC 1 cut(s) 2297
AspS9I GGNCC 2 cut(s) 40, 2398
AsuC2I CCSGG 1 cut(s) 1306
AsuHPI GGTGA 7 cut(s) 533, 683, 1000, 1193, 2114, 2405, 2456
AvaII GGWCC 2 cut(s) 40, 2398
BalI TGGCCA 2 cut(s) 1148, 2264
BanI GGYRCC 1 cut(s) 2297
BanII GRGCYC 6 cut(s) 314, 1081, 1299, 1384, 1602, 1859
BauI CACGAG 1 cut(s) 1808
Bbv12I GWGCWC 4 cut(s) 1081, 1299, 1602, 1755
BbvI GCAGC 6 cut(s) 420, 1086, 1855, 1922, 1991, 2278
BccI CCATC 7 cut(s) 181, 442, 512, 902, 1688, 1986, 2092
BciT130I CCWGG 2 cut(s) 937, 2052
BciVI GTATCC 1 cut(s) 2171
BclI TGATCA 3 cut(s) 249, 1815, 2154
BcnI CCSGG 1 cut(s) 1306
BcoDI GTCTC 3 cut(s) 479, 1524, 2060
BfaI CTAG 4 cut(s) 590, 720, 1460, 1778
BfmI CTRYAG 2 cut(s) 16, 2292
BfuAI ACCTGC 2 cut(s) 1613, 2285
BfuI GTATCC 1 cut(s) 2171
BisI GCNGC 7 cut(s) 409, 1100, 1497, 1869, 1911, 1980, 2292
BlsI GCNGC 7 cut(s) 410, 1101, 1498, 1870, 1912, 1981, 2293
BmcAI AGTACT 1 cut(s) 2287
Bme1390I CCNGG 3 cut(s) 937, 1306, 2052
Bme18I GGWCC 2 cut(s) 40, 2398
BmgT120I GGNCC 2 cut(s) 40, 2398
BmiI GGNNCC 8 cut(s) 1381, 1740, 1836, 1858, 1935, 2038, 2299, 2399
BmrFI CCNGG 3 cut(s) 937, 1306, 2052
BmsI GCATC 4 cut(s) 687, 1483, 1751, 1991
BpmI CTGGAG 1 cut(s) 1031
BpuEI CTTGAG 2 cut(s) 428, 1946
BpuMI CCSGG 1 cut(s) 1306
BsaI GGTCTC 2 cut(s) 479, 2060
BsaJI CCNNGG 3 cut(s) 490, 1830, 2265
BsaXI ACNNNNNCTCC 4 cut(s) 54, 84, 1007, 1037
Bsc4I CCNNNNNNNGG 5 cut(s) 49, 1312, 1390, 1946, 2307
Bse1I ACTGG 2 cut(s) 1564, 2265
Bse3DI GCAATG 5 cut(s) 544, 700, 762, 795, 976
BseBI CCWGG 2 cut(s) 937, 2052
BseDI CCNNGG 3 cut(s) 490, 1830, 2265
BseGI GGATG 3 cut(s) 523, 1314, 1766
BseLI CCNNNNNNNGG 5 cut(s) 49, 1312, 1390, 1946, 2307
BseMI GCAATG 5 cut(s) 544, 700, 762, 795, 976
BseMII CTCAG 5 cut(s) 180, 932, 1516, 2071, 2139
BseNI ACTGG 2 cut(s) 1564, 2265
BseRI GAGGAG 1 cut(s) 1028
BseXI GCAGC 6 cut(s) 420, 1086, 1855, 1922, 1991, 2278
BseYI CCCAGC 5 cut(s) 212, 228, 1679, 1787, 2006
BsgI GTGCAG 2 cut(s) 1389, 2100
Bsh1236I CGCG 1 cut(s) 953
BshFI GGCC 5 cut(s) 449, 1148, 1330, 1782, 2264
BshNI GGYRCC 1 cut(s) 2297
BsiHKAI GWGCWC 4 cut(s) 1081, 1299, 1602, 1755
BsiSI CCGG 1 cut(s) 1306
BslFI GGGAC 3 cut(s) 6, 1875, 2411
BslI CCNNNNNNNGG 5 cut(s) 49, 1312, 1390, 1946, 2307
BsmAI GTCTC 3 cut(s) 479, 1524, 2060
BsmFI GGGAC 3 cut(s) 6, 1875, 2411
BsmI GAATGC 3 cut(s) 960, 1011, 1552
BsnI GGCC 5 cut(s) 449, 1148, 1330, 1782, 2264
Bso31I GGTCTC 2 cut(s) 479, 2060
Bsp1286I GDGCHC 7 cut(s) 314, 1081, 1299, 1384, 1602, 1755, 1859
Bsp143I GATC 9 cut(s) 249, 546, 948, 1251, 1387, 1554, 1815, 2154, 2429
Bsp19I CCATGG 2 cut(s) 490, 2265
BspACI CCGC 6 cut(s) 510, 953, 1496, 1947, 2117, 2237
BspANI GGCC 5 cut(s) 449, 1148, 1330, 1782, 2264
BspCNI CTCAG 5 cut(s) 179, 931, 1515, 2070, 2140
BspFNI CGCG 1 cut(s) 953
BspHI TCATGA 3 cut(s) 1233, 2086, 2094
BspLI GGNNCC 8 cut(s) 1381, 1740, 1836, 1858, 1935, 2038, 2299, 2399
BspMAI CTGCAG 1 cut(s) 2296
BspMI ACCTGC 2 cut(s) 1613, 2285
BspPI GGATC 2 cut(s) 541, 2437
BspT107I GGYRCC 1 cut(s) 2297
BspTNI GGTCTC 2 cut(s) 479, 2060
BsrDI GCAATG 5 cut(s) 544, 700, 762, 795, 976
BsrI ACTGG 2 cut(s) 1564, 2265
BssECI CCNNGG 3 cut(s) 490, 1830, 2265
BssMI GATC 9 cut(s) 249, 546, 948, 1251, 1387, 1554, 1815, 2154, 2429
BssSI CACGAG 1 cut(s) 1808
BssT1I CCWWGG 2 cut(s) 490, 2265
Bst2BI CACGAG 1 cut(s) 1808
Bst2UI CCWGG 2 cut(s) 937, 2052
Bst4CI ACNGT 4 cut(s) 17, 1535, 2342, 2402
Bst6I CTCTTC 3 cut(s) 475, 975, 2217
BstAPI GCANNNNNTGC 3 cut(s) 706, 794, 966
BstDEI CTNAG 6 cut(s) 166, 918, 1448, 1502, 2057, 2148
BstDSI CCRYGG 3 cut(s) 490, 1830, 2265
BstF5I GGATG 3 cut(s) 523, 1314, 1766
BstFNI CGCG 1 cut(s) 953
BstKTI GATC 9 cut(s) 252, 549, 951, 1254, 1390, 1557, 1818, 2157, 2432
BstMAI GTCTC 3 cut(s) 479, 1524, 2060
BstMBI GATC 9 cut(s) 249, 546, 948, 1251, 1387, 1554, 1815, 2154, 2429
BstNI CCWGG 2 cut(s) 937, 2052
BstNSI RCATGY 4 cut(s) 797, 1106, 1471, 1828
BstSCI CCNGG 3 cut(s) 935, 1304, 2050
BstSFI CTRYAG 2 cut(s) 16, 2292
BstUI CGCG 1 cut(s) 953
BstV1I GCAGC 6 cut(s) 420, 1086, 1855, 1922, 1991, 2278
BstX2I RGATCY 1 cut(s) 2429
BstXI CCANNNNNNTGG 1 cut(s) 1156
BstYI RGATCY 1 cut(s) 2429
BsuI GTATCC 1 cut(s) 2171
BsuRI GGCC 5 cut(s) 449, 1148, 1330, 1782, 2264
BtgI CCRYGG 3 cut(s) 490, 1830, 2265
BtgZI GCGATG 1 cut(s) 959
BtsCI GGATG 3 cut(s) 523, 1314, 1766
BtsI GCAGTG 1 cut(s) 1149
BtsIMutI CAGTG 2 cut(s) 1149, 1557
BveI ACCTGC 2 cut(s) 1613, 2285
CciI TCATGA 3 cut(s) 1233, 2086, 2094
Cfr13I GGNCC 2 cut(s) 40, 2398
CseI GACGC 1 cut(s) 533
CsiI ACCWGGT 1 cut(s) 2050
Csp6I GTAC 5 cut(s) 633, 1278, 1721, 2286, 2298
CspCI CAANNNNNGTGG 2 cut(s) 433, 468
CviQI GTAC 5 cut(s) 633, 1278, 1721, 2286, 2298
DdeI CTNAG 6 cut(s) 166, 918, 1448, 1502, 2057, 2148
DpnI GATC 9 cut(s) 251, 548, 950, 1253, 1389, 1556, 1817, 2156, 2431
DpnII GATC 9 cut(s) 249, 546, 948, 1251, 1387, 1554, 1815, 2154, 2429
DraIII CACNNNGTG 1 cut(s) 1367
EaeI YGGCCR 2 cut(s) 1146, 2262
Eam1104I CTCTTC 3 cut(s) 475, 975, 2217
EarI CTCTTC 3 cut(s) 475, 975, 2217
Ecl136II GAGCTC 3 cut(s) 1079, 1297, 1600
Eco130I CCWWGG 2 cut(s) 490, 2265
Eco147I AGGCCT 1 cut(s) 449
Eco24I GRGCYC 6 cut(s) 314, 1081, 1299, 1384, 1602, 1859
Eco31I GGTCTC 2 cut(s) 479, 2060
Eco32I GATATC 1 cut(s) 1526
Eco47I GGWCC 2 cut(s) 40, 2398
Eco53kI GAGCTC 3 cut(s) 1079, 1297, 1600
Eco57I CTGAAG 2 cut(s) 300, 1923
EcoICRI GAGCTC 3 cut(s) 1079, 1297, 1600
EcoRII CCWGG 2 cut(s) 935, 2050
EcoRV GATATC 1 cut(s) 1526
EcoT14I CCWWGG 2 cut(s) 490, 2265
EcoT22I ATGCAT 5 cut(s) 544, 702, 795, 1575, 1766
EcoT38I GRGCYC 6 cut(s) 314, 1081, 1299, 1384, 1602, 1859
ErhI CCWWGG 2 cut(s) 490, 2265
FalI AAGNNNNNCTT 2 cut(s) 181, 213
FaqI GGGAC 3 cut(s) 6, 1875, 2411
FauI CCCGC 2 cut(s) 946, 2124
FbaI TGATCA 3 cut(s) 249, 1815, 2154
Fnu4HI GCNGC 7 cut(s) 409, 1100, 1497, 1869, 1911, 1980, 2292
FokI GGATG 3 cut(s) 530, 1321, 1773
FriOI GRGCYC 6 cut(s) 314, 1081, 1299, 1384, 1602, 1859
Fsp4HI GCNGC 7 cut(s) 409, 1100, 1497, 1869, 1911, 1980, 2292
FspBI CTAG 4 cut(s) 590, 720, 1460, 1778
GluI GCNGC 7 cut(s) 409, 1100, 1497, 1869, 1911, 1980, 2292
GsaI CCCAGC 5 cut(s) 216, 232, 1683, 1791, 2010
GsuI CTGGAG 1 cut(s) 1031
HaeIII GGCC 5 cut(s) 449, 1148, 1330, 1782, 2264
HapII CCGG 1 cut(s) 1306
HgaI GACGC 1 cut(s) 533
HincII GTYRAC 1 cut(s) 418
HindII GTYRAC 1 cut(s) 418
HindIII AAGCTT 2 cut(s) 459, 1590
HinfI GANTC 2 cut(s) 1061, 1888
HpaII CCGG 1 cut(s) 1306
HphI GGTGA 7 cut(s) 533, 683, 1000, 1193, 2114, 2405, 2456
Hpy166II GTNNAC 3 cut(s) 418, 1465, 2242
Hpy188I TCNGA 6 cut(s) 169, 921, 1294, 1962, 2310, 2327
Hpy188III TCNNGA 9 cut(s) 352, 1082, 1234, 1529, 1711, 1808, 2087, 2095, 2158
Hpy8I GTNNAC 3 cut(s) 418, 1465, 2242
HpyAV CCTTC 7 cut(s) 98, 284, 324, 888, 1306, 1696, 2420
HpyCH4III ACNGT 4 cut(s) 17, 1535, 2342, 2402
HpyCH4IV ACGT 1 cut(s) 1956
HpyF3I CTNAG 6 cut(s) 166, 918, 1448, 1502, 2057, 2148
HpySE526I ACGT 1 cut(s) 1956
KpnI GGTACC 1 cut(s) 2301
Ksp22I TGATCA 3 cut(s) 249, 1815, 2154
Kzo9I GATC 9 cut(s) 249, 546, 948, 1251, 1387, 1554, 1815, 2154, 2429
LmnI GCTCC 8 cut(s) 570, 1076, 1274, 1294, 1379, 1744, 1834, 1862
Lsp1109I GCAGC 6 cut(s) 420, 1086, 1855, 1922, 1991, 2278
LweI GCATC 4 cut(s) 687, 1483, 1751, 1991
MabI ACCWGGT 1 cut(s) 2050
MaeI CTAG 4 cut(s) 590, 720, 1460, 1778
MaeII ACGT 1 cut(s) 1956
MaeIII GTNAC 6 cut(s) 52, 521, 841, 1339, 2342, 2444
MalI GATC 9 cut(s) 251, 548, 950, 1253, 1389, 1556, 1817, 2156, 2431
MboI GATC 9 cut(s) 249, 546, 948, 1251, 1387, 1554, 1815, 2154, 2429
MboII GAAGA 8 cut(s) 72, 116, 411, 492, 992, 1234, 1619, 2234
MfeI CAATTG 3 cut(s) 1273, 1845, 2064
MflI RGATCY 1 cut(s) 2429
MhlI GDGCHC 7 cut(s) 314, 1081, 1299, 1384, 1602, 1755, 1859
MlsI TGGCCA 2 cut(s) 1148, 2264
MluNI TGGCCA 2 cut(s) 1148, 2264
MlyI GAGTC 2 cut(s) 1070, 1897
MmeI TCCRAC 3 cut(s) 136, 1054, 1911
Mox20I TGGCCA 2 cut(s) 1148, 2264
Mph1103I ATGCAT 5 cut(s) 544, 702, 795, 1575, 1766
MroXI GAANNNNTTC 1 cut(s) 1706
MscI TGGCCA 2 cut(s) 1148, 2264
MseI TTAA 2 cut(s) 642, 999
MslI CAYNNNNRTG 5 cut(s) 392, 798, 1107, 1578, 1829
Msp20I TGGCCA 2 cut(s) 1148, 2264
MspI CCGG 1 cut(s) 1306
MspR9I CCNGG 3 cut(s) 937, 1306, 2052
MunI CAATTG 3 cut(s) 1273, 1845, 2064
Mva1269I GAATGC 3 cut(s) 960, 1011, 1552
MvaI CCWGG 2 cut(s) 937, 2052
MvnI CGCG 1 cut(s) 953
NciI CCSGG 1 cut(s) 1306
NcoI CCATGG 2 cut(s) 490, 2265
NdeII GATC 9 cut(s) 249, 546, 948, 1251, 1387, 1554, 1815, 2154, 2429
NlaIV GGNNCC 8 cut(s) 1381, 1740, 1836, 1858, 1935, 2038, 2299, 2399
NmeAIII GCCGAG 2 cut(s) 1235, 1808
NmuCI GTSAC 4 cut(s) 52, 521, 2342, 2444
NsiI ATGCAT 5 cut(s) 544, 702, 795, 1575, 1766
NspI RCATGY 4 cut(s) 797, 1106, 1471, 1828
PaeI GCATGC 3 cut(s) 797, 1106, 1828
PagI TCATGA 3 cut(s) 1233, 2086, 2094
PaqCI CACCTGC 1 cut(s) 1613
PceI AGGCCT 1 cut(s) 449
PciI ACATGT 1 cut(s) 1467
PcsI WCGNNNNNNNCGW 2 cut(s) 1534, 1635
PctI GAATGC 3 cut(s) 960, 1011, 1552
PdmI GAANNNNTTC 1 cut(s) 1706
PflMI CCANNNNNTGG 1 cut(s) 49
PfoI TCCNGGA 1 cut(s) 935
PkrI GCNGC 7 cut(s) 410, 1101, 1498, 1870, 1912, 1981, 2293
PleI GAGTC 2 cut(s) 1069, 1896
PpsI GAGTC 2 cut(s) 1069, 1896
PscI ACATGT 1 cut(s) 1467
PshBI ATTAAT 1 cut(s) 642
PsiI TTATAA 2 cut(s) 689, 1953
Psp124BI GAGCTC 3 cut(s) 1081, 1299, 1602
Psp1406I AACGTT 1 cut(s) 1956
Psp6I CCWGG 2 cut(s) 935, 2050
PspFI CCCAGC 5 cut(s) 212, 228, 1679, 1787, 2006
PspGI CCWGG 2 cut(s) 935, 2050
PspN4I GGNNCC 8 cut(s) 1381, 1740, 1836, 1858, 1935, 2038, 2299, 2399
PspPI GGNCC 2 cut(s) 40, 2398
PstI CTGCAG 1 cut(s) 2296
PsuI RGATCY 1 cut(s) 2429
RsaI GTAC 5 cut(s) 634, 1279, 1722, 2287, 2299
RsaNI GTAC 5 cut(s) 633, 1278, 1721, 2286, 2298
RseI CAYNNNNRTG 5 cut(s) 392, 798, 1107, 1578, 1829
SacI GAGCTC 3 cut(s) 1081, 1299, 1602
SaqAI TTAA 2 cut(s) 642, 999
SatI GCNGC 7 cut(s) 409, 1100, 1497, 1869, 1911, 1980, 2292
Sau3AI GATC 9 cut(s) 249, 546, 948, 1251, 1387, 1554, 1815, 2154, 2429
Sau96I GGNCC 2 cut(s) 40, 2398
ScaI AGTACT 1 cut(s) 2287
SchI GAGTC 2 cut(s) 1070, 1897
ScrFI CCNGG 3 cut(s) 937, 1306, 2052
SduI GDGCHC 7 cut(s) 314, 1081, 1299, 1384, 1602, 1755, 1859
SexAI ACCWGGT 1 cut(s) 2050
SfaNI GCATC 4 cut(s) 687, 1483, 1751, 1991
SfcI CTRYAG 2 cut(s) 16, 2292
SinI GGWCC 2 cut(s) 40, 2398
SmiMI CAYNNNNRTG 5 cut(s) 392, 798, 1107, 1578, 1829
SmlI CTYRAG 2 cut(s) 443, 1925
SmoI CTYRAG 2 cut(s) 443, 1925
SphI GCATGC 3 cut(s) 797, 1106, 1828
SseBI AGGCCT 1 cut(s) 449
SsiI CCGC 6 cut(s) 510, 953, 1496, 1947, 2117, 2237
SspMI CTAG 4 cut(s) 590, 720, 1460, 1778
SstI GAGCTC 3 cut(s) 1081, 1299, 1602
StuI AGGCCT 1 cut(s) 449
StyD4I CCNGG 3 cut(s) 935, 1304, 2050
StyI CCWWGG 2 cut(s) 490, 2265
TaaI ACNGT 4 cut(s) 17, 1535, 2342, 2402
TaiI ACGT 1 cut(s) 1959
TaqI TCGA 6 cut(s) 1059, 1528, 1537, 1629, 1710, 1797
TatI WGTACW 2 cut(s) 1277, 2285
TauI GCSGC 1 cut(s) 1499
Tru1I TTAA 2 cut(s) 642, 999
Tru9I TTAA 2 cut(s) 642, 999
TscAI CASTG 2 cut(s) 1156, 1564
TseFI GTSAC 4 cut(s) 52, 521, 2342, 2444
TseI GCWGC 6 cut(s) 408, 1099, 1868, 1910, 1979, 2291
Tsp45I GTSAC 4 cut(s) 52, 521, 2342, 2444
TspGWI ACGGA 1 cut(s) 1441
TspRI CASTG 2 cut(s) 1156, 1564
Van91I CCANNNNNTGG 1 cut(s) 49
VpaK11BI GGWCC 2 cut(s) 40, 2398
VspI ATTAAT 1 cut(s) 642
XapI RAATTY 3 cut(s) 880, 1878, 2130
XceI RCATGY 4 cut(s) 797, 1106, 1471, 1828
XmnI GAANNNNTTC 1 cut(s) 1706
XspI CTAG 4 cut(s) 590, 720, 1460, 1778
ZrmI AGTACT 1 cut(s) 2287
Zsp2I ATGCAT 5 cut(s) 544, 702, 795, 1575, 1766
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.