MD12G1260900.v1.1

2-alkenal reductase (NADP(

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0017133
Physical Location & Seq
Forward (+)
32717741 .. 32729034
11294 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1260900.v1.1.491

Sequence Viewer

Length: 1047 bp
ATGGCTGAAAAAGTACAAGTAGTAGAGAACAAGCAAGTGATCTTCAAGGGGTTCGTTGAGGGAACTCCAAAGGAGACGGACTTGCAAGTGTTGGTCGGCGCCACGACTGAGCTCAAGCCTCCTAAAGGCTCCGGAGCTTTTCTGGTCAAGAATCTCTACCTCTCTTGCGACCCTTACATGAGAGGCCGTATGCGCGATTTTGGTGATTCCTATATTCCTCCATTCGTTCCTGGCCAGCCAATCGAAGGATTTGGTGTGTCCAAAGTTGTGGGTTCTGATAATCCGACTTTCAAGCCTGGCGACTTCATCTCTGGGTTCACTAGATGGGAAGAATACAGTTTGATTTCTAGAACTGAGCAGTTGAGAAAAGTTACGCCAGACGGCGACATCCCTCTATCATTCCATGTGGGTCTTCTTGGTATGCCAGGTTTTACTGCATATGCAGGATTCTACGAGGTCTGCTCCCCTAAGAAGGGGGAATGTGTTTTAGTTTCTGCTGCTTCCGGAGCTGTTGGTCAGCTTGTTGGCCAACTTGCTAAATTGCATGGCTGTTATGTTGTTGGAAGTTGTGGCTCAAGCCAAAAAGTTGATCTTCTGAGGAATAAGCTTGGGTTTGATGAAGCTTTTAACTACAAAGAAGAGGCAGACCTGGATGCAGCTTTGAAAAGGTACTTTCCACAAGGCATTGACATTTACTTTGATAATGTGGGAGGGGACATGCTTGATGCAGCACTTGTGAACATGAGAATTCACGGTCGGATTGCTGTTTGTGGAATGGTGTCTGAGAAAAACCTCTCTGATCCCCGAGGGATCCGCAATGTATTCAATCTGATATCAAAGCGTATCAGGATGCAGGGATTCCTGCAAAGTGATTACATACACTTGTACCCACGCTTCTTGGAACATGTCATTAGTTACTACAAGCAAGGGAAGATTATTTACATTGAAGACATGAATGAAGGCTTGGAAAGTGCTCCATCTGCTTTTGTAGGCTTATTTGCGGGAACAAATGTTGGTAAGCAAGTTATTCGTGTAGCCCACGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ADH_N_2 ADH_zinc_N

Protein Analysis

349

Amino Acids

38.36

Weight (kDa)

6.61

Isoelectric Point (pI)

33.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ADH_N_2 PF16884 11 - 124 1.3e-25 N-terminal domain of oxidoreductase
ADH_zinc_N PF00107 170 - 301 7.5e-25 Zinc-binding dehydrogenase
ADH_zinc_N_2 PF13602 203 - 343 3.6e-07 Zinc-binding dehydrogenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017133)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G65560
fragaria_vesca FvH4_6g00020
malus_domestica MD12G1260900.v1.1
prunus_persica Prupe.6G365900_v2.0.a1
pyrus_communis pycom12g24290
rosa_chinensis RchiOBHm_Chr3g0447341
rosa_laevigata RLG00000026009
rosa_roxburghii Rroxscaffold_6G00425520
rosa_rugosa Rorug02G0600100
rosa_samantha Rh3AG000400 Rh3CG000200 Rh3DG000400
rosa_wichuraiana Rw3G000020

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 98
AccII CGCG 1 cut(s) 195
AccIII TCCGGA 2 cut(s) 131, 503
AciI CCGC 2 cut(s) 814, 1001
AclWI GGATC 3 cut(s) 794, 805, 818
AcoI YGGCCR 2 cut(s) 232, 526
AcsI RAATTY 1 cut(s) 747
AcyI GRCGYC 1 cut(s) 99
AfaI GTAC 3 cut(s) 15, 671, 887
AfiI CCNNNNNNNGG 4 cut(s) 125, 245, 472, 473
AflIII ACRYGT 1 cut(s) 904
AgsI TTSAA 5 cut(s) 46, 292, 664, 826, 947
AjnI CCWGG 4 cut(s) 229, 295, 424, 648
AjuI GAANNNNNNNTTGG 2 cut(s) 947, 979
AluBI AGCT 7 cut(s) 112, 137, 509, 520, 607, 623, 659
AluI AGCT 7 cut(s) 112, 137, 509, 520, 607, 623, 659
Alw21I GWGCWC 2 cut(s) 114, 976
Alw26I GTCTC 1 cut(s) 68
AlwI GGATC 3 cut(s) 794, 805, 818
Ama87I CYCGRG 1 cut(s) 804
Aor13HI TCCGGA 2 cut(s) 131, 503
AoxI GGCC 3 cut(s) 184, 232, 526
ApeKI GCWGC 3 cut(s) 497, 656, 728
ApoI RAATTY 1 cut(s) 747
ArsI GACNNNNNNTTYG 2 cut(s) 680, 712
AspLEI GCGC 2 cut(s) 101, 195
AsuHPI GGTGA 1 cut(s) 215
AvaI CYCGRG 1 cut(s) 804
BaeI ACNNNNGTAYC 2 cut(s) 869, 902
BalI TGGCCA 2 cut(s) 234, 528
BamHI GGATCC 1 cut(s) 810
BanI GGYRCC 1 cut(s) 98
BanII GRGCYC 1 cut(s) 114
BarI GAAGNNNNNNTAC 4 cut(s) 878, 910, 923, 955
BbsI GAAGAC 2 cut(s) 404, 954
Bbv12I GWGCWC 2 cut(s) 114, 976
BbvI GCAGC 3 cut(s) 484, 668, 740
BccI CCATC 2 cut(s) 318, 985
BceAI ACGGC 2 cut(s) 171, 397
BcgI CGANNNNNNTGC 2 cut(s) 1010, 1044
BciT130I CCWGG 4 cut(s) 231, 297, 426, 650
BcoDI GTCTC 1 cut(s) 68
BfaI CTAG 2 cut(s) 321, 348
BfoI RGCGCY 1 cut(s) 102
BisI GCNGC 3 cut(s) 498, 657, 729
BlsI GCNGC 3 cut(s) 499, 658, 730
Bme1390I CCNGG 4 cut(s) 231, 297, 426, 650
BmeT110I CYCGRG 1 cut(s) 804
BmiI GGNNCC 3 cut(s) 100, 130, 812
BmrFI CCNGG 4 cut(s) 231, 297, 426, 650
BmsI GCATC 3 cut(s) 643, 715, 840
BpiI GAAGAC 2 cut(s) 404, 954
BplI GAGNNNNNCTC 2 cut(s) 446, 478
BpuEI CTTGAG 2 cut(s) 98, 559
BsaHI GRCGYC 1 cut(s) 99
BsaJI CCNNGG 1 cut(s) 805
BsaWI WCCGGW 2 cut(s) 131, 503
Bsc4I CCNNNNNNNGG 4 cut(s) 125, 245, 472, 473
Bse3DI GCAATG 1 cut(s) 823
BseAI TCCGGA 2 cut(s) 131, 503
BseBI CCWGG 4 cut(s) 231, 297, 426, 650
BseDI CCNNGG 1 cut(s) 805
BseGI GGATG 3 cut(s) 387, 658, 855
BseLI CCNNNNNNNGG 4 cut(s) 125, 245, 472, 473
BseMI GCAATG 1 cut(s) 823
BseMII CTCAG 4 cut(s) 99, 345, 587, 774
BseXI GCAGC 3 cut(s) 484, 668, 740
Bsh1236I CGCG 1 cut(s) 195
Bsh1285I CGRYCG 1 cut(s) 757
BshFI GGCC 3 cut(s) 186, 234, 528
BshNI GGYRCC 1 cut(s) 98
BsiEI CGRYCG 1 cut(s) 757
BsiHKAI GWGCWC 2 cut(s) 114, 976
BsiHKCI CYCGRG 1 cut(s) 804
BsiSI CCGG 2 cut(s) 132, 504
BslFI GGGAC 1 cut(s) 728
BslI CCNNNNNNNGG 4 cut(s) 125, 245, 472, 473
BsmAI GTCTC 1 cut(s) 68
BsmBI CGTCTC 1 cut(s) 68
BsmFI GGGAC 1 cut(s) 728
BsnI GGCC 3 cut(s) 186, 234, 528
BsoBI CYCGRG 1 cut(s) 804
Bsp1286I GDGCHC 2 cut(s) 114, 976
Bsp13I TCCGGA 2 cut(s) 131, 503
Bsp143I GATC 4 cut(s) 39, 589, 799, 810
BspACI CCGC 2 cut(s) 814, 1001
BspANI GGCC 3 cut(s) 186, 234, 528
BspCNI CTCAG 4 cut(s) 100, 346, 588, 775
BspEI TCCGGA 2 cut(s) 131, 503
BspFNI CGCG 1 cut(s) 195
BspLI GGNNCC 3 cut(s) 100, 130, 812
BspPI GGATC 3 cut(s) 794, 805, 818
BspT107I GGYRCC 1 cut(s) 98
BsrDI GCAATG 1 cut(s) 823
BssECI CCNNGG 1 cut(s) 805
BssMI GATC 4 cut(s) 39, 589, 799, 810
BssNI GRCGYC 1 cut(s) 99
Bst2UI CCWGG 4 cut(s) 231, 297, 426, 650
Bst4CI ACNGT 2 cut(s) 338, 755
Bst6I CTCTTC 1 cut(s) 633
BstACI GRCGYC 1 cut(s) 99
BstC8I GCNNGC 1 cut(s) 236
BstDEI CTNAG 5 cut(s) 108, 354, 468, 596, 783
BstENI CCTNNNNNAGG 1 cut(s) 123
BstF5I GGATG 3 cut(s) 387, 658, 855
BstFNI CGCG 1 cut(s) 195
BstH2I RGCGCY 1 cut(s) 102
BstHHI GCGC 2 cut(s) 101, 195
BstKTI GATC 4 cut(s) 42, 592, 802, 813
BstMAI GTCTC 1 cut(s) 68
BstMBI GATC 4 cut(s) 39, 589, 799, 810
BstMCI CGRYCG 1 cut(s) 757
BstMWI GCNNNNNNNGC 3 cut(s) 192, 506, 980
BstNI CCWGG 4 cut(s) 231, 297, 426, 650
BstNSI RCATGY 2 cut(s) 721, 908
BstSCI CCNGG 4 cut(s) 229, 295, 424, 648
BstUI CGCG 1 cut(s) 195
BstV1I GCAGC 3 cut(s) 484, 668, 740
BstV2I GAAGAC 2 cut(s) 404, 954
BstX2I RGATCY 1 cut(s) 810
BstXI CCANNNNNNTGG 1 cut(s) 268
BstYI RGATCY 1 cut(s) 810
BsuRI GGCC 3 cut(s) 186, 234, 528
BtsCI GGATG 3 cut(s) 387, 658, 855
Cac8I GCNNGC 1 cut(s) 236
CfoI GCGC 2 cut(s) 101, 195
Csp6I GTAC 3 cut(s) 14, 670, 886
CspCI CAANNNNNGTGG 2 cut(s) 879, 914
CviAII CATG 7 cut(s) 178, 404, 545, 718, 742, 905, 952
CviQI GTAC 3 cut(s) 14, 670, 886
DdeI CTNAG 5 cut(s) 108, 354, 468, 596, 783
DinI GGCGCC 1 cut(s) 100
DpnI GATC 4 cut(s) 41, 591, 801, 812
DpnII GATC 4 cut(s) 39, 589, 799, 810
EaeI YGGCCR 2 cut(s) 232, 526
Eam1104I CTCTTC 1 cut(s) 633
EarI CTCTTC 1 cut(s) 633
Ecl136II GAGCTC 1 cut(s) 112
Eco24I GRGCYC 1 cut(s) 114
Eco32I GATATC 1 cut(s) 834
Eco53kI GAGCTC 1 cut(s) 112
Eco88I CYCGRG 1 cut(s) 804
EcoICRI GAGCTC 1 cut(s) 112
EcoNI CCTNNNNNAGG 1 cut(s) 123
EcoRI GAATTC 1 cut(s) 747
EcoRII CCWGG 4 cut(s) 229, 295, 424, 648
EcoRV GATATC 1 cut(s) 834
EcoT38I GRGCYC 1 cut(s) 114
EgeI GGCGCC 1 cut(s) 100
EheI GGCGCC 1 cut(s) 100
Esp3I CGTCTC 1 cut(s) 68
FaeI CATG 7 cut(s) 181, 407, 548, 721, 745, 908, 955
FalI AAGNNNNNCTT 2 cut(s) 576, 608
FaqI GGGAC 1 cut(s) 728
FatI CATG 7 cut(s) 177, 403, 544, 717, 741, 904, 951
FauI CCCGC 1 cut(s) 994
FauNDI CATATG 1 cut(s) 439
Fnu4HI GCNGC 3 cut(s) 498, 657, 729
FokI GGATG 3 cut(s) 374, 665, 862
FriOI GRGCYC 1 cut(s) 114
Fsp4HI GCNGC 3 cut(s) 498, 657, 729
FspBI CTAG 2 cut(s) 321, 348
GlaI GCGC 2 cut(s) 100, 194
GluI GCNGC 3 cut(s) 498, 657, 729
HaeII RGCGCY 1 cut(s) 102
HaeIII GGCC 3 cut(s) 186, 234, 528
HapII CCGG 2 cut(s) 132, 504
HhaI GCGC 2 cut(s) 101, 195
Hin1I GRCGYC 1 cut(s) 99
Hin1II CATG 7 cut(s) 181, 407, 548, 721, 745, 908, 955
Hin6I GCGC 2 cut(s) 99, 193
HinP1I GCGC 2 cut(s) 99, 193
HindIII AAGCTT 2 cut(s) 605, 621
HinfI GANTC 4 cut(s) 151, 206, 447, 858
HpaII CCGG 2 cut(s) 132, 504
HphI GGTGA 1 cut(s) 215
Hpy166II GTNNAC 2 cut(s) 318, 739
Hpy188I TCNGA 7 cut(s) 277, 285, 597, 759, 784, 799, 831
Hpy188III TCNNGA 5 cut(s) 132, 148, 348, 504, 847
Hpy8I GTNNAC 2 cut(s) 318, 739
HpyAV CCTTC 3 cut(s) 239, 466, 953
HpyCH4III ACNGT 2 cut(s) 338, 755
HpyCH4V TGCA 8 cut(s) 85, 437, 443, 544, 656, 728, 853, 865
HpyF10VI GCNNNNNNNGC 3 cut(s) 192, 506, 980
HpyF3I CTNAG 5 cut(s) 108, 354, 468, 596, 783
Hsp92I GRCGYC 1 cut(s) 99
Hsp92II CATG 7 cut(s) 181, 407, 548, 721, 745, 908, 955
HspAI GCGC 2 cut(s) 99, 193
KasI GGCGCC 1 cut(s) 98
Kpn2I TCCGGA 2 cut(s) 131, 503
Kzo9I GATC 4 cut(s) 39, 589, 799, 810
LmnI GCTCC 5 cut(s) 134, 134, 467, 506, 979
Lsp1109I GCAGC 3 cut(s) 484, 668, 740
LweI GCATC 3 cut(s) 643, 715, 840
MaeI CTAG 2 cut(s) 321, 348
MaeIII GTNAC 2 cut(s) 370, 914
MalI GATC 4 cut(s) 41, 591, 801, 812
MboI GATC 4 cut(s) 39, 589, 799, 810
MboII GAAGA 7 cut(s) 34, 341, 404, 584, 650, 943, 959
MflI RGATCY 1 cut(s) 810
MhlI GDGCHC 2 cut(s) 114, 976
MlsI TGGCCA 2 cut(s) 234, 528
MluCI AATT 2 cut(s) 539, 747
MluNI TGGCCA 2 cut(s) 234, 528
Mly113I GGCGCC 1 cut(s) 99
MmeI TCCRAC 3 cut(s) 308, 541, 737
Mox20I TGGCCA 2 cut(s) 234, 528
MroI TCCGGA 2 cut(s) 131, 503
MscI TGGCCA 2 cut(s) 234, 528
MseI TTAA 1 cut(s) 627
Msp20I TGGCCA 2 cut(s) 234, 528
MspI CCGG 2 cut(s) 132, 504
MspR9I CCNGG 4 cut(s) 231, 297, 426, 650
MvaI CCWGG 4 cut(s) 231, 297, 426, 650
MvnI CGCG 1 cut(s) 195
MwoI GCNNNNNNNGC 3 cut(s) 192, 506, 980
NarI GGCGCC 1 cut(s) 99
NdeI CATATG 1 cut(s) 439
NdeII GATC 4 cut(s) 39, 589, 799, 810
NlaIII CATG 7 cut(s) 181, 407, 548, 721, 745, 908, 955
NlaIV GGNNCC 3 cut(s) 100, 130, 812
NspI RCATGY 2 cut(s) 721, 908
PciI ACATGT 1 cut(s) 904
PfeI GAWTC 4 cut(s) 151, 206, 447, 858
PkrI GCNGC 3 cut(s) 499, 658, 730
PluTI GGCGCC 1 cut(s) 102
PscI ACATGT 1 cut(s) 904
Psp124BI GAGCTC 1 cut(s) 114
Psp6I CCWGG 4 cut(s) 229, 295, 424, 648
PspGI CCWGG 4 cut(s) 229, 295, 424, 648
PspN4I GGNNCC 3 cut(s) 100, 130, 812
PsuI RGATCY 1 cut(s) 810
RsaI GTAC 3 cut(s) 15, 671, 887
RsaNI GTAC 3 cut(s) 14, 670, 886
SacI GAGCTC 1 cut(s) 114
SaqAI TTAA 1 cut(s) 627
SatI GCNGC 3 cut(s) 498, 657, 729
Sau3AI GATC 4 cut(s) 39, 589, 799, 810
ScrFI CCNGG 4 cut(s) 231, 297, 426, 650
SduI GDGCHC 2 cut(s) 114, 976
SfaNI GCATC 3 cut(s) 643, 715, 840
SfoI GGCGCC 1 cut(s) 100
SmlI CTYRAG 2 cut(s) 113, 574
SmoI CTYRAG 2 cut(s) 113, 574
Sse9I AATT 2 cut(s) 539, 747
SsiI CCGC 2 cut(s) 814, 1001
SspDI GGCGCC 1 cut(s) 98
SspMI CTAG 2 cut(s) 321, 348
SstI GAGCTC 1 cut(s) 114
StyD4I CCNGG 4 cut(s) 229, 295, 424, 648
TaaI ACNGT 2 cut(s) 338, 755
TaqI TCGA 1 cut(s) 243
TasI AATT 2 cut(s) 539, 747
TatI WGTACW 1 cut(s) 13
TfiI GAWTC 4 cut(s) 151, 206, 447, 858
Tru1I TTAA 1 cut(s) 627
Tru9I TTAA 1 cut(s) 627
TseI GCWGC 3 cut(s) 497, 656, 728
TspDTI ATGAA 4 cut(s) 295, 633, 968, 972
TspGWI ACGGA 1 cut(s) 92
XagI CCTNNNNNAGG 1 cut(s) 123
XapI RAATTY 1 cut(s) 747
XbaI TCTAGA 1 cut(s) 347
XceI RCATGY 2 cut(s) 721, 908
XspI CTAG 2 cut(s) 321, 348
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.