RLG00000026009

2-alkenal reductase (NADP(

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0017133
Physical Location & Seq
Forward (+)
49865582 .. 49868664
3083 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000026009

Sequence Viewer

Length: 1044 bp
ATGGTGGTAGCAATGGAAGCAGAGAACAAACAAGTTATCTTCAAGGGGTTCATAGAGGGCACTCCAAAGGAGACAGACTTACAAGTGATGGCTGGGATTAGTATCGAGCTCAAGGCTCCTAAAGGCTCTGGAGCTTTCCTGGTCAAGAATCTCTACCTCTCTTGTGACCCTTATATGAGGGGACGTATGCGTGATTTTCGTGATTCATATATCCCTCCATTCGTTCCTGGCAAGCCACTTGAAGGATTTGGTGTCTCCCAAGTTGTGGATTCTGATAATCCGAGTTTCAAGCCTGGCGACTTGATCTCTGGCATAACCGGATGGGAAGAATACAGCTTGATTCGTAGAACTGAGCAGTTGAGAAAAATTAAGCAAGATGACATCCCTCTATCGTTCCATTTGGGTCTTCTTGGTATGCCAGGTTTTACTGCATATGCAGGATTCAATGAGGTCTGCTCTCCTAAGAAAGGGGAATATGTTTTAGTTTCTGCTGCTTCTGGAGCCGTTGGTCAGCTTGTTGGTCAGCTTGCTAAGTTGCTTGGCTGTCACGTAGTTGGAAGTTCTGGTTCAAGCCAAAAGGTTGATCTTCTGAGGAATAAGCTTGGTTTTGATGTTGCTTTTAACTACAAGGAAGAGCCAGACCTCGATGCAGCTCTGAAAAGGTACTTTCCTCAAGGCATTGACATCTACTTTGATAATGTGGGAGGGGAAATGCTTGATGCAGCGCTTCTCAACATGAGAATTCATGGTCGAATTGCTGTTTGTGGGATGGTGTCTGAAAAAAATCTCTCTGATCCCGGAGGGATCCACAATTTATACAATCTCATAACAAAGCGCATCAGGATGCAAGGATTTCTGCAAAGTGATTACTTGCACTTGTACCCGCGTTTCTTGGAAGATGTTATTAGCCTCTACAAGCAAGGGAAGATTGTTTACATTGAAGACATGAATGAAGGCTTGGAAAGTGCTCCATCTGCTTTTGTTGGGTTATTTTCGGGAAGAAATATTGGCAAGCAAGTCATTCGTGTTGCAAGATATGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ADH_N_2 ADH_zinc_N

Protein Analysis

348

Amino Acids

38.37

Weight (kDa)

6.55

Isoelectric Point (pI)

39.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ADH_N_2 PF16884 10 - 120 2.5e-25 N-terminal domain of oxidoreductase
ADH_zinc_N PF00107 168 - 291 3.2e-25 Zinc-binding dehydrogenase
ADH_zinc_N_2 PF13602 201 - 341 1.3e-07 Zinc-binding dehydrogenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017133)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G65560
fragaria_vesca FvH4_6g00020
malus_domestica MD12G1260900.v1.1
prunus_persica Prupe.6G365900_v2.0.a1
pyrus_communis pycom12g24290
rosa_chinensis RchiOBHm_Chr3g0447341
rosa_laevigata RLG00000026009
rosa_roxburghii Rroxscaffold_6G00425520
rosa_rugosa Rorug02G0600100
rosa_samantha Rh3AG000400 Rh3CG000200 Rh3DG000400
rosa_wichuraiana Rw3G000020

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 265
AccII CGCG 1 cut(s) 886
AciI CCGC 1 cut(s) 884
AclWI GGATC 3 cut(s) 788, 799, 812
AcsI RAATTY 1 cut(s) 741
AfaI GTAC 2 cut(s) 665, 881
AfeI AGCGCT 1 cut(s) 726
AfiI CCNNNNNNNGG 3 cut(s) 242, 265, 467
AgsI TTSAA 6 cut(s) 43, 242, 289, 445, 570, 941
AjnI CCWGG 4 cut(s) 138, 226, 292, 418
AjuI GAANNNNNNNTTGG 2 cut(s) 941, 973
AluBI AGCT 7 cut(s) 109, 134, 336, 514, 526, 601, 653
AluI AGCT 7 cut(s) 109, 134, 336, 514, 526, 601, 653
Alw21I GWGCWC 2 cut(s) 111, 970
Alw26I GTCTC 2 cut(s) 65, 259
AlwI GGATC 3 cut(s) 788, 799, 812
Aor51HI AGCGCT 1 cut(s) 726
ApeKI GCWGC 3 cut(s) 491, 650, 722
ApoI RAATTY 1 cut(s) 741
ArsI GACNNNNNNTTYG 2 cut(s) 674, 706
AspLEI GCGC 2 cut(s) 727, 837
AsuC2I CCSGG 1 cut(s) 798
BaeGI GKGCMC 1 cut(s) 62
BamHI GGATCC 1 cut(s) 804
BanII GRGCYC 1 cut(s) 111
BarI GAAGNNNNNNTAC 2 cut(s) 917, 949
BbsI GAAGAC 2 cut(s) 398, 948
Bbv12I GWGCWC 2 cut(s) 111, 970
BbvI GCAGC 3 cut(s) 478, 662, 734
BccI CCATC 4 cut(s) 82, 315, 763, 979
BceAI ACGGC 1 cut(s) 488
BcgI CGANNNNNNTGC 2 cut(s) 1004, 1038
BciT130I CCWGG 4 cut(s) 140, 228, 294, 420
BcnI CCSGG 1 cut(s) 798
BcoDI GTCTC 2 cut(s) 65, 259
BfoI RGCGCY 1 cut(s) 728
BisI GCNGC 3 cut(s) 492, 651, 723
BlsI GCNGC 3 cut(s) 493, 652, 724
Bme1390I CCNGG 5 cut(s) 140, 228, 294, 420, 798
BmiI GGNNCC 3 cut(s) 117, 502, 806
BmrFI CCNGG 5 cut(s) 140, 228, 294, 420, 798
BmsI GCATC 4 cut(s) 637, 709, 834, 846
BpiI GAAGAC 2 cut(s) 398, 948
BplI GAGNNNNNCTC 2 cut(s) 440, 472
BpmI CTGGAG 2 cut(s) 150, 519
BpuEI CTTGAG 2 cut(s) 95, 657
BpuMI CCSGG 1 cut(s) 798
BsaAI YACGTR 1 cut(s) 550
BsaBI GATNNNNATC 1 cut(s) 101
BsaWI WCCGGW 1 cut(s) 317
Bsc4I CCNNNNNNNGG 3 cut(s) 242, 265, 467
Bse3DI GCAATG 1 cut(s) 18
Bse8I GATNNNNATC 1 cut(s) 101
BseBI CCWGG 4 cut(s) 140, 228, 294, 420
BseGI GGATG 4 cut(s) 326, 381, 774, 849
BseJI GATNNNNATC 1 cut(s) 101
BseLI CCNNNNNNNGG 3 cut(s) 242, 265, 467
BseMI GCAATG 1 cut(s) 18
BseMII CTCAG 2 cut(s) 342, 581
BseSI GKGCMC 1 cut(s) 62
BseXI GCAGC 3 cut(s) 478, 662, 734
BseYI CCCAGC 1 cut(s) 92
Bsh1236I CGCG 1 cut(s) 886
BsiHKAI GWGCWC 2 cut(s) 111, 970
BsiSI CCGG 2 cut(s) 318, 798
BslFI GGGAC 1 cut(s) 195
BslI CCNNNNNNNGG 3 cut(s) 242, 265, 467
BsmAI GTCTC 2 cut(s) 65, 259
BsmFI GGGAC 1 cut(s) 195
Bsp1286I GDGCHC 3 cut(s) 62, 111, 970
Bsp143I GATC 4 cut(s) 303, 583, 793, 804
BspACI CCGC 1 cut(s) 884
BspCNI CTCAG 2 cut(s) 343, 582
BspFNI CGCG 1 cut(s) 886
BspLI GGNNCC 3 cut(s) 117, 502, 806
BspPI GGATC 3 cut(s) 788, 799, 812
BspQI GCTCTTC 1 cut(s) 627
BsrDI GCAATG 1 cut(s) 18
BssMI GATC 4 cut(s) 303, 583, 793, 804
Bst2UI CCWGG 4 cut(s) 140, 228, 294, 420
Bst6I CTCTTC 1 cut(s) 627
BstBAI YACGTR 1 cut(s) 550
BstC8I GCNNGC 3 cut(s) 233, 528, 1013
BstDEI CTNAG 4 cut(s) 351, 462, 531, 590
BstENI CCTNNNNNAGG 1 cut(s) 465
BstF5I GGATG 4 cut(s) 326, 381, 774, 849
BstFNI CGCG 1 cut(s) 886
BstH2I RGCGCY 1 cut(s) 728
BstHHI GCGC 2 cut(s) 727, 837
BstKTI GATC 4 cut(s) 306, 586, 796, 807
BstMAI GTCTC 2 cut(s) 65, 259
BstMBI GATC 4 cut(s) 303, 583, 793, 804
BstMWI GCNNNNNNNGC 3 cut(s) 17, 500, 974
BstNI CCWGG 4 cut(s) 140, 228, 294, 420
BstSCI CCNGG 5 cut(s) 138, 226, 292, 418, 796
BstSLI GKGCMC 1 cut(s) 62
BstUI CGCG 1 cut(s) 886
BstV1I GCAGC 3 cut(s) 478, 662, 734
BstV2I GAAGAC 2 cut(s) 398, 948
BstX2I RGATCY 1 cut(s) 804
BstYI RGATCY 1 cut(s) 804
BtsCI GGATG 4 cut(s) 326, 381, 774, 849
Cac8I GCNNGC 3 cut(s) 233, 528, 1013
CfoI GCGC 2 cut(s) 727, 837
Csp6I GTAC 2 cut(s) 664, 880
CviAII CATG 3 cut(s) 736, 746, 946
CviQI GTAC 2 cut(s) 664, 880
DdeI CTNAG 4 cut(s) 351, 462, 531, 590
DpnI GATC 4 cut(s) 305, 585, 795, 806
DpnII GATC 4 cut(s) 303, 583, 793, 804
Eam1104I CTCTTC 1 cut(s) 627
EarI CTCTTC 1 cut(s) 627
Ecl136II GAGCTC 1 cut(s) 109
Eco24I GRGCYC 1 cut(s) 111
Eco47III AGCGCT 1 cut(s) 726
Eco53kI GAGCTC 1 cut(s) 109
EcoICRI GAGCTC 1 cut(s) 109
EcoNI CCTNNNNNAGG 1 cut(s) 465
EcoRI GAATTC 1 cut(s) 741
EcoRII CCWGG 4 cut(s) 138, 226, 292, 418
EcoT38I GRGCYC 1 cut(s) 111
FaeI CATG 3 cut(s) 739, 749, 949
FaqI GGGAC 1 cut(s) 195
FatI CATG 3 cut(s) 735, 745, 945
FauI CCCGC 1 cut(s) 891
FauNDI CATATG 1 cut(s) 433
Fnu4HI GCNGC 3 cut(s) 492, 651, 723
FokI GGATG 4 cut(s) 333, 368, 781, 856
FriOI GRGCYC 1 cut(s) 111
Fsp4HI GCNGC 3 cut(s) 492, 651, 723
GlaI GCGC 2 cut(s) 726, 836
GluI GCNGC 3 cut(s) 492, 651, 723
GsaI CCCAGC 1 cut(s) 96
GsuI CTGGAG 2 cut(s) 150, 519
HaeII RGCGCY 1 cut(s) 728
HapII CCGG 2 cut(s) 318, 798
HhaI GCGC 2 cut(s) 727, 837
Hin1II CATG 3 cut(s) 739, 749, 949
Hin6I GCGC 2 cut(s) 725, 835
HinP1I GCGC 2 cut(s) 725, 835
HindIII AAGCTT 1 cut(s) 599
HinfI GANTC 5 cut(s) 148, 203, 269, 340, 441
HpaII CCGG 2 cut(s) 318, 798
Hpy166II GTNNAC 1 cut(s) 934
Hpy188I TCNGA 6 cut(s) 274, 282, 591, 657, 778, 793
Hpy188III TCNNGA 6 cut(s) 129, 145, 200, 498, 841, 996
Hpy8I GTNNAC 1 cut(s) 934
HpyAV CCTTC 2 cut(s) 236, 947
HpyCH4IV ACGT 2 cut(s) 184, 549
HpyCH4V TGCA 8 cut(s) 431, 437, 650, 722, 847, 859, 874, 1031
HpyF10VI GCNNNNNNNGC 3 cut(s) 17, 500, 974
HpyF3I CTNAG 4 cut(s) 351, 462, 531, 590
HpySE526I ACGT 2 cut(s) 184, 549
Hsp92II CATG 3 cut(s) 739, 749, 949
HspAI GCGC 2 cut(s) 725, 835
Kzo9I GATC 4 cut(s) 303, 583, 793, 804
LguI GCTCTTC 1 cut(s) 627
LmnI GCTCC 4 cut(s) 121, 131, 500, 973
Lsp1109I GCAGC 3 cut(s) 478, 662, 734
LweI GCATC 4 cut(s) 637, 709, 834, 846
MaeII ACGT 2 cut(s) 184, 549
MaeIII GTNAC 2 cut(s) 164, 545
MalI GATC 4 cut(s) 305, 585, 795, 806
MboI GATC 4 cut(s) 303, 583, 793, 804
MboII GAAGA 9 cut(s) 31, 338, 398, 578, 644, 908, 937, 953, 1011
MflI RGATCY 1 cut(s) 804
MhlI GDGCHC 3 cut(s) 62, 111, 970
MluCI AATT 4 cut(s) 366, 741, 753, 811
MmeI TCCRAC 1 cut(s) 535
MseI TTAA 2 cut(s) 369, 621
MslI CAYNNNNRTG 1 cut(s) 842
MspI CCGG 2 cut(s) 318, 798
MspR9I CCNGG 5 cut(s) 140, 228, 294, 420, 798
MvaI CCWGG 4 cut(s) 140, 228, 294, 420
MvnI CGCG 1 cut(s) 886
MwoI GCNNNNNNNGC 3 cut(s) 17, 500, 974
NciI CCSGG 1 cut(s) 798
NdeI CATATG 1 cut(s) 433
NdeII GATC 4 cut(s) 303, 583, 793, 804
NlaIII CATG 3 cut(s) 739, 749, 949
NlaIV GGNNCC 3 cut(s) 117, 502, 806
NmuCI GTSAC 2 cut(s) 164, 545
PciSI GCTCTTC 1 cut(s) 627
PfeI GAWTC 5 cut(s) 148, 203, 269, 340, 441
PflMI CCANNNNNTGG 1 cut(s) 265
PfoI TCCNGGA 1 cut(s) 796
PkrI GCNGC 3 cut(s) 493, 652, 724
Ppu21I YACGTR 1 cut(s) 550
Psp124BI GAGCTC 1 cut(s) 111
Psp6I CCWGG 4 cut(s) 138, 226, 292, 418
PspFI CCCAGC 1 cut(s) 92
PspGI CCWGG 4 cut(s) 138, 226, 292, 418
PspN4I GGNNCC 3 cut(s) 117, 502, 806
PsuI RGATCY 1 cut(s) 804
RsaI GTAC 2 cut(s) 665, 881
RsaNI GTAC 2 cut(s) 664, 880
RseI CAYNNNNRTG 1 cut(s) 842
SacI GAGCTC 1 cut(s) 111
SapI GCTCTTC 1 cut(s) 627
SaqAI TTAA 2 cut(s) 369, 621
SatI GCNGC 3 cut(s) 492, 651, 723
Sau3AI GATC 4 cut(s) 303, 583, 793, 804
ScrFI CCNGG 5 cut(s) 140, 228, 294, 420, 798
SduI GDGCHC 3 cut(s) 62, 111, 970
SfaNI GCATC 4 cut(s) 637, 709, 834, 846
SmiMI CAYNNNNRTG 1 cut(s) 842
SmlI CTYRAG 2 cut(s) 110, 672
SmoI CTYRAG 2 cut(s) 110, 672
Sse9I AATT 4 cut(s) 366, 741, 753, 811
SsiI CCGC 1 cut(s) 884
SspI AATATT 1 cut(s) 1006
SstI GAGCTC 1 cut(s) 111
StyD4I CCNGG 5 cut(s) 138, 226, 292, 418, 796
TaiI ACGT 2 cut(s) 187, 552
TaqI TCGA 3 cut(s) 105, 645, 751
TasI AATT 4 cut(s) 366, 741, 753, 811
TfiI GAWTC 5 cut(s) 148, 203, 269, 340, 441
Tru1I TTAA 2 cut(s) 369, 621
Tru9I TTAA 2 cut(s) 369, 621
TseFI GTSAC 2 cut(s) 164, 545
TseI GCWGC 3 cut(s) 491, 650, 722
Tsp45I GTSAC 2 cut(s) 164, 545
TspDTI ATGAA 5 cut(s) 40, 195, 734, 962, 966
Van91I CCANNNNNTGG 1 cut(s) 265
XagI CCTNNNNNAGG 1 cut(s) 465
XapI RAATTY 1 cut(s) 741
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.