pycom12g24290

2-alkenal reductase (NADP(

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0017133
Physical Location & Seq
Forward (+)
24643995 .. 24646342
2348 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12g24290.3

Sequence Viewer

Length: 1011 bp
ATGGCTGAAAAAGTACTAGTAGAGAACAAGCAAGTGATATTCAAGGGGTTCGTAGAGGGAACTCCAAAGGAGACGGACTTGCAAGTGGTGGTCAGCGCCACGACTGAGCTCAAGCCTCCTAAAGGCTCCGGAGCTTTTCTGGTCAAGAATCTCTACCTCTCTTGCGACCCTTACATGAGAGGCCGTATGCGCGATTTTCGTGATTCCTATATTCCTCCATTCGTTCCTGGCCAGCCTGGCGACTTGATCTCTGGGTTCACTGGATGGGAAGAATACAGTTTGATTTCTAGAACTGAGCAGTTGAGAAAAGTTACGCCAGACGGCGACATCCCTCTATCATTCCATGTGGGTCTTCTTGGTATGCCAGGTTTTACTGCATATGCAGGATTCTACGAGGTCTGCTCCCCTAAGAAGGGGGAATGTGTTTTAGTTTCTGCTGCTTCCGGAGCTGTTGGTCAGCTTGTTGGCCAACTTGCTAAATTGCATGGCTGTTATGTTGTTGGAAGTTGTGGCTCAAGCCAAAAGGTAAGGTCTCATTTGTGTTTCTCTGTTGATCTTCTGAGGAATAAGCTTGGGTTTGATGAAGCTTTTAACTACAAAGAAGAGGCAGACCTGGATGCAGCTTTGAAAAGGTACTTTCCACAAGGCATTGACATTTACTTTGATAATGTGGGAGGGGACATGCTTGATGCAGCAATTGTGAACATGAGAATTCACGGTCGGATTGCTGTTTGTGGAATGGTGTCTGAGAAAAACCTCTCTGATCCCCGAGGGATCCGCAATGTATTCAATCTGATATCAAAGCGTATCAGGATGCAGGGATTCCTGCAAAGTGATTACATACACTTGTACCCACGCTTCTTGGAACATGTCATTAGTTACTACAAGCAAGGGAAGATTATTTACATTGAAGACATGAATGAAGGCTTGGAAAGTGCTCCATCTGCTTTTGTAGGCTTATTTGCGGGAACAAATGTTGGTAAGCAAGTTATTCGTGTAGCCCACGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ADH_N_2 ADH_zinc_N

Protein Analysis

337

Amino Acids

37.19

Weight (kDa)

7.04

Isoelectric Point (pI)

34.36

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017133)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G65560
fragaria_vesca FvH4_6g00020
malus_domestica MD12G1260900.v1.1
prunus_persica Prupe.6G365900_v2.0.a1
pyrus_communis pycom12g24290
rosa_chinensis RchiOBHm_Chr3g0447341
rosa_laevigata RLG00000026009
rosa_roxburghii Rroxscaffold_6G00425520
rosa_rugosa Rorug02G0600100
rosa_samantha Rh3AG000400 Rh3CG000200 Rh3DG000400
rosa_wichuraiana Rw3G000020

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 192
AccIII TCCGGA 2 cut(s) 128, 443
AciI CCGC 2 cut(s) 778, 965
AclWI GGATC 3 cut(s) 758, 769, 782
AcoI YGGCCR 2 cut(s) 229, 466
AcsI RAATTY 1 cut(s) 711
AfaI GTAC 3 cut(s) 15, 635, 851
AfiI CCNNNNNNNGG 3 cut(s) 122, 412, 413
AflIII ACRYGT 1 cut(s) 868
AgsI TTSAA 4 cut(s) 43, 628, 790, 911
AhlI ACTAGT 1 cut(s) 16
AjnI CCWGG 4 cut(s) 226, 235, 364, 612
AjuI GAANNNNNNNTTGG 2 cut(s) 911, 943
AluBI AGCT 7 cut(s) 109, 134, 449, 460, 571, 587, 623
AluI AGCT 7 cut(s) 109, 134, 449, 460, 571, 587, 623
Alw21I GWGCWC 2 cut(s) 111, 940
Alw26I GTCTC 2 cut(s) 65, 537
AlwI GGATC 3 cut(s) 758, 769, 782
Ama87I CYCGRG 1 cut(s) 768
Aor13HI TCCGGA 2 cut(s) 128, 443
AoxI GGCC 3 cut(s) 181, 229, 466
ApeKI GCWGC 3 cut(s) 437, 620, 692
ApoI RAATTY 1 cut(s) 711
ArsI GACNNNNNNTTYG 2 cut(s) 644, 676
AspLEI GCGC 2 cut(s) 98, 192
AvaI CYCGRG 1 cut(s) 768
BaeI ACNNNNGTAYC 2 cut(s) 833, 866
BalI TGGCCA 2 cut(s) 231, 468
BamHI GGATCC 1 cut(s) 774
BanII GRGCYC 1 cut(s) 111
BarI GAAGNNNNNNTAC 4 cut(s) 842, 874, 887, 919
BbsI GAAGAC 2 cut(s) 344, 918
Bbv12I GWGCWC 2 cut(s) 111, 940
BbvI GCAGC 3 cut(s) 424, 632, 704
BccI CCATC 2 cut(s) 258, 949
BceAI ACGGC 2 cut(s) 168, 337
BcgI CGANNNNNNTGC 2 cut(s) 974, 1008
BciT130I CCWGG 4 cut(s) 228, 237, 366, 614
BcoDI GTCTC 2 cut(s) 65, 537
BcuI ACTAGT 1 cut(s) 16
BfaI CTAG 2 cut(s) 17, 288
BfoI RGCGCY 1 cut(s) 99
BglI GCCNNNNNGGC 1 cut(s) 237
BisI GCNGC 3 cut(s) 438, 621, 693
BlsI GCNGC 3 cut(s) 439, 622, 694
BmcAI AGTACT 1 cut(s) 15
Bme1390I CCNGG 4 cut(s) 228, 237, 366, 614
BmeT110I CYCGRG 1 cut(s) 768
BmiI GGNNCC 2 cut(s) 127, 776
BmrFI CCNGG 4 cut(s) 228, 237, 366, 614
BmsI GCATC 3 cut(s) 607, 679, 804
BpiI GAAGAC 2 cut(s) 344, 918
BplI GAGNNNNNCTC 2 cut(s) 386, 418
BpuEI CTTGAG 2 cut(s) 95, 499
BsaI GGTCTC 1 cut(s) 537
BsaJI CCNNGG 1 cut(s) 769
BsaWI WCCGGW 2 cut(s) 128, 443
Bsc4I CCNNNNNNNGG 3 cut(s) 122, 412, 413
Bse1I ACTGG 1 cut(s) 265
Bse3DI GCAATG 1 cut(s) 787
BseAI TCCGGA 2 cut(s) 128, 443
BseBI CCWGG 4 cut(s) 228, 237, 366, 614
BseDI CCNNGG 1 cut(s) 769
BseGI GGATG 4 cut(s) 269, 327, 622, 819
BseLI CCNNNNNNNGG 3 cut(s) 122, 412, 413
BseMI GCAATG 1 cut(s) 787
BseMII CTCAG 4 cut(s) 96, 285, 551, 738
BseNI ACTGG 1 cut(s) 265
BseXI GCAGC 3 cut(s) 424, 632, 704
Bsh1236I CGCG 1 cut(s) 192
Bsh1285I CGRYCG 1 cut(s) 721
BshFI GGCC 3 cut(s) 183, 231, 468
BsiEI CGRYCG 1 cut(s) 721
BsiHKAI GWGCWC 2 cut(s) 111, 940
BsiHKCI CYCGRG 1 cut(s) 768
BsiSI CCGG 2 cut(s) 129, 444
BslFI GGGAC 1 cut(s) 692
BslI CCNNNNNNNGG 3 cut(s) 122, 412, 413
BsmAI GTCTC 2 cut(s) 65, 537
BsmBI CGTCTC 1 cut(s) 65
BsmFI GGGAC 1 cut(s) 692
BsnI GGCC 3 cut(s) 183, 231, 468
Bso31I GGTCTC 1 cut(s) 537
BsoBI CYCGRG 1 cut(s) 768
Bsp1286I GDGCHC 2 cut(s) 111, 940
Bsp13I TCCGGA 2 cut(s) 128, 443
Bsp143I GATC 4 cut(s) 246, 553, 763, 774
BspACI CCGC 2 cut(s) 778, 965
BspANI GGCC 3 cut(s) 183, 231, 468
BspCNI CTCAG 4 cut(s) 97, 286, 552, 739
BspEI TCCGGA 2 cut(s) 128, 443
BspFNI CGCG 1 cut(s) 192
BspLI GGNNCC 2 cut(s) 127, 776
BspPI GGATC 3 cut(s) 758, 769, 782
BspTNI GGTCTC 1 cut(s) 537
BsrDI GCAATG 1 cut(s) 787
BsrI ACTGG 1 cut(s) 265
BssECI CCNNGG 1 cut(s) 769
BssMI GATC 4 cut(s) 246, 553, 763, 774
Bst2UI CCWGG 4 cut(s) 228, 237, 366, 614
Bst4CI ACNGT 2 cut(s) 278, 719
Bst6I CTCTTC 1 cut(s) 597
BstC8I GCNNGC 1 cut(s) 233
BstDEI CTNAG 5 cut(s) 105, 294, 408, 560, 747
BstENI CCTNNNNNAGG 1 cut(s) 120
BstF5I GGATG 4 cut(s) 269, 327, 622, 819
BstFNI CGCG 1 cut(s) 192
BstH2I RGCGCY 1 cut(s) 99
BstHHI GCGC 2 cut(s) 98, 192
BstKTI GATC 4 cut(s) 249, 556, 766, 777
BstMAI GTCTC 2 cut(s) 65, 537
BstMBI GATC 4 cut(s) 246, 553, 763, 774
BstMCI CGRYCG 1 cut(s) 721
BstMWI GCNNNNNNNGC 4 cut(s) 189, 237, 446, 944
BstNI CCWGG 4 cut(s) 228, 237, 366, 614
BstNSI RCATGY 2 cut(s) 685, 872
BstSCI CCNGG 4 cut(s) 226, 235, 364, 612
BstUI CGCG 1 cut(s) 192
BstV1I GCAGC 3 cut(s) 424, 632, 704
BstV2I GAAGAC 2 cut(s) 344, 918
BstX2I RGATCY 1 cut(s) 774
BstYI RGATCY 1 cut(s) 774
BsuRI GGCC 3 cut(s) 183, 231, 468
BtsCI GGATG 4 cut(s) 269, 327, 622, 819
BtsIMutI CAGTG 1 cut(s) 258
Cac8I GCNNGC 1 cut(s) 233
CfoI GCGC 2 cut(s) 98, 192
Csp6I GTAC 3 cut(s) 14, 634, 850
CspCI CAANNNNNGTGG 2 cut(s) 843, 878
CviAII CATG 7 cut(s) 175, 344, 485, 682, 706, 869, 916
CviQI GTAC 3 cut(s) 14, 634, 850
DdeI CTNAG 5 cut(s) 105, 294, 408, 560, 747
DpnI GATC 4 cut(s) 248, 555, 765, 776
DpnII GATC 4 cut(s) 246, 553, 763, 774
EaeI YGGCCR 2 cut(s) 229, 466
Eam1104I CTCTTC 1 cut(s) 597
EarI CTCTTC 1 cut(s) 597
Ecl136II GAGCTC 1 cut(s) 109
Eco24I GRGCYC 1 cut(s) 111
Eco31I GGTCTC 1 cut(s) 537
Eco32I GATATC 1 cut(s) 798
Eco53kI GAGCTC 1 cut(s) 109
Eco88I CYCGRG 1 cut(s) 768
EcoICRI GAGCTC 1 cut(s) 109
EcoNI CCTNNNNNAGG 1 cut(s) 120
EcoRI GAATTC 1 cut(s) 711
EcoRII CCWGG 4 cut(s) 226, 235, 364, 612
EcoRV GATATC 1 cut(s) 798
EcoT38I GRGCYC 1 cut(s) 111
Esp3I CGTCTC 1 cut(s) 65
FaeI CATG 7 cut(s) 178, 347, 488, 685, 709, 872, 919
FaqI GGGAC 1 cut(s) 692
FatI CATG 7 cut(s) 174, 343, 484, 681, 705, 868, 915
FauI CCCGC 1 cut(s) 958
FauNDI CATATG 1 cut(s) 379
Fnu4HI GCNGC 3 cut(s) 438, 621, 693
FokI GGATG 4 cut(s) 276, 314, 629, 826
FriOI GRGCYC 1 cut(s) 111
Fsp4HI GCNGC 3 cut(s) 438, 621, 693
FspBI CTAG 2 cut(s) 17, 288
GlaI GCGC 2 cut(s) 97, 191
GluI GCNGC 3 cut(s) 438, 621, 693
HaeII RGCGCY 1 cut(s) 99
HaeIII GGCC 3 cut(s) 183, 231, 468
HapII CCGG 2 cut(s) 129, 444
HhaI GCGC 2 cut(s) 98, 192
Hin1II CATG 7 cut(s) 178, 347, 488, 685, 709, 872, 919
Hin6I GCGC 2 cut(s) 96, 190
HinP1I GCGC 2 cut(s) 96, 190
HindIII AAGCTT 2 cut(s) 569, 585
HinfI GANTC 4 cut(s) 148, 203, 387, 822
HpaII CCGG 2 cut(s) 129, 444
Hpy166II GTNNAC 2 cut(s) 258, 703
Hpy188I TCNGA 5 cut(s) 561, 723, 748, 763, 795
Hpy188III TCNNGA 6 cut(s) 129, 145, 200, 288, 444, 811
Hpy8I GTNNAC 2 cut(s) 258, 703
HpyAV CCTTC 2 cut(s) 406, 917
HpyCH4III ACNGT 2 cut(s) 278, 719
HpyCH4V TGCA 8 cut(s) 82, 377, 383, 484, 620, 692, 817, 829
HpyF10VI GCNNNNNNNGC 4 cut(s) 189, 237, 446, 944
HpyF3I CTNAG 5 cut(s) 105, 294, 408, 560, 747
Hsp92II CATG 7 cut(s) 178, 347, 488, 685, 709, 872, 919
HspAI GCGC 2 cut(s) 96, 190
Kpn2I TCCGGA 2 cut(s) 128, 443
Kzo9I GATC 4 cut(s) 246, 553, 763, 774
LmnI GCTCC 5 cut(s) 131, 131, 407, 446, 943
Lsp1109I GCAGC 3 cut(s) 424, 632, 704
LweI GCATC 3 cut(s) 607, 679, 804
MaeI CTAG 2 cut(s) 17, 288
MaeIII GTNAC 2 cut(s) 310, 878
MalI GATC 4 cut(s) 248, 555, 765, 776
MboI GATC 4 cut(s) 246, 553, 763, 774
MboII GAAGA 6 cut(s) 281, 344, 548, 614, 907, 923
MfeI CAATTG 1 cut(s) 696
MflI RGATCY 1 cut(s) 774
MhlI GDGCHC 2 cut(s) 111, 940
MlsI TGGCCA 2 cut(s) 231, 468
MluCI AATT 3 cut(s) 479, 696, 711
MluNI TGGCCA 2 cut(s) 231, 468
MmeI TCCRAC 2 cut(s) 481, 701
Mox20I TGGCCA 2 cut(s) 231, 468
MroI TCCGGA 2 cut(s) 128, 443
MscI TGGCCA 2 cut(s) 231, 468
MseI TTAA 1 cut(s) 591
Msp20I TGGCCA 2 cut(s) 231, 468
MspI CCGG 2 cut(s) 129, 444
MspR9I CCNGG 4 cut(s) 228, 237, 366, 614
MunI CAATTG 1 cut(s) 696
MvaI CCWGG 4 cut(s) 228, 237, 366, 614
MvnI CGCG 1 cut(s) 192
MwoI GCNNNNNNNGC 4 cut(s) 189, 237, 446, 944
NdeI CATATG 1 cut(s) 379
NdeII GATC 4 cut(s) 246, 553, 763, 774
NlaIII CATG 7 cut(s) 178, 347, 488, 685, 709, 872, 919
NlaIV GGNNCC 2 cut(s) 127, 776
NspI RCATGY 2 cut(s) 685, 872
PciI ACATGT 1 cut(s) 868
PfeI GAWTC 4 cut(s) 148, 203, 387, 822
PkrI GCNGC 3 cut(s) 439, 622, 694
PscI ACATGT 1 cut(s) 868
Psp124BI GAGCTC 1 cut(s) 111
Psp6I CCWGG 4 cut(s) 226, 235, 364, 612
PspGI CCWGG 4 cut(s) 226, 235, 364, 612
PspN4I GGNNCC 2 cut(s) 127, 776
PsuI RGATCY 1 cut(s) 774
RsaI GTAC 3 cut(s) 15, 635, 851
RsaNI GTAC 3 cut(s) 14, 634, 850
SacI GAGCTC 1 cut(s) 111
SaqAI TTAA 1 cut(s) 591
SatI GCNGC 3 cut(s) 438, 621, 693
Sau3AI GATC 4 cut(s) 246, 553, 763, 774
ScaI AGTACT 1 cut(s) 15
ScrFI CCNGG 4 cut(s) 228, 237, 366, 614
SduI GDGCHC 2 cut(s) 111, 940
SfaNI GCATC 3 cut(s) 607, 679, 804
SmlI CTYRAG 2 cut(s) 110, 514
SmoI CTYRAG 2 cut(s) 110, 514
SpeI ACTAGT 1 cut(s) 16
Sse9I AATT 3 cut(s) 479, 696, 711
SsiI CCGC 2 cut(s) 778, 965
SspMI CTAG 2 cut(s) 17, 288
SstI GAGCTC 1 cut(s) 111
StyD4I CCNGG 4 cut(s) 226, 235, 364, 612
TaaI ACNGT 2 cut(s) 278, 719
TasI AATT 3 cut(s) 479, 696, 711
TatI WGTACW 1 cut(s) 13
TfiI GAWTC 4 cut(s) 148, 203, 387, 822
Tru1I TTAA 1 cut(s) 591
Tru9I TTAA 1 cut(s) 591
TscAI CASTG 1 cut(s) 265
TseI GCWGC 3 cut(s) 437, 620, 692
TspDTI ATGAA 3 cut(s) 597, 932, 936
TspGWI ACGGA 1 cut(s) 89
TspRI CASTG 1 cut(s) 265
XagI CCTNNNNNAGG 1 cut(s) 120
XapI RAATTY 1 cut(s) 711
XbaI TCTAGA 1 cut(s) 287
XceI RCATGY 2 cut(s) 685, 872
XspI CTAG 2 cut(s) 17, 288
ZrmI AGTACT 1 cut(s) 15
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.