MD13G1016200.v1.1

Hydroxyproline-rich glycoprotein family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0008548
Physical Location & Seq
Reverse (-)
1032989 .. 1035515
2527 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1016200.v1.1.491

Sequence Viewer

Length: 1092 bp
ATGAGGGGACATGGAAGAGAAATGATTCAATTGGGCCTACCTGTTATTGATACTCCATCTGAAGATGAAATTTCGTCAGGAACCTCCAAAGATCGGAGAATAGAAGCTATCCCTTCCTTGCTGCAGGATGTTATTGATCGCATAGCTGGGATGCAAGTTACTACTGTGAAGCCAGACTCTTGTATCATTGATTTCTATAATGAGGGAGATCATTCACACCCTCACACGTGGCCACCTTGGTTTGGAAGGCCTATTTGCGTCTTGTTCCTGACAGAATGTGACATGACTTTTGGGAGAGTACTAGTATCAGACCATCCTGGGGATTATAGAGGCCCACTCAAGCTCTCTCTTACACCTGGATCCCTCCTTTTGCTGCAAGGGAAATCCACAGACTTTGCTAAACATGCAATACCTTCTATACGGAAGCAACGCGTACTTGTTACTTTCACAAAGTCTCAACCAAAGAAAAATACGATGAGCGATGGTCAACGTTTTCCTGCACCTACTCCAGCTCAGTCATCCTACTGGGGTCAACCATCAGGTAGGTCTCCAAGCCACATTCGGCATCCTGCTGGTCCCAAGCACTATGCAGCAGTTCCGACAACTGGTGTGTTGCCAGCCCCACCAATTCGGTCCCAACTTCCACCTCCAAATGGCATCCAGCCATTGTTTGTGCCACCTCCGGTAGGACCACCAGCAATACCTTTTGCCGGAGCAGTTTCCATCCCACCTGTTTCGGCTGGATGGGCAGCAGCTCCCAGGCATCCTCCACCTCGCATCCCTCCTCCTGGCACTGGTGTTTTCCTCCCTCCCCCAGGTTCGGGTAACTCATCAGCTCCTCAACAGCTGCCTACTACTGCAACTCAAATGAGCCCTTCTGTTGAGATTCCTCCCCAGACAGAGAGAGAGAGCGGGTCTGCAAAATCCAACCATAGCACAACTCCTCCAAAAGGAAAATCGGATGGGAAAGCTCAGAGCCATGAGTGCAATGGAAGTCTGGATGGAACTGGCAGTGGAAGAGCAGCCGTTAAGGAAGAAGAAGATCAGCACTCCGACAGCATGACAACAAGCGATCAAGCCGGAGCAGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy_2

Protein Analysis

364

Amino Acids

38.53

Weight (kDa)

8.33

Isoelectric Point (pI)

58.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
2OG-FeII_Oxy_2 PF13532 29 - 152 5.2e-06 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 912
AccII CGCG 1 cut(s) 432
AciI CCGC 1 cut(s) 912
AclI AACGTT 1 cut(s) 490
AclWI GGATC 2 cut(s) 354, 367
AcoI YGGCCR 1 cut(s) 230
AcsI RAATTY 1 cut(s) 69
AcuI CTGAAG 1 cut(s) 81
AcvI CACGTG 1 cut(s) 228
AfaI GTAC 2 cut(s) 300, 435
AflIII ACRYGT 2 cut(s) 225, 430
AgsI TTSAA 1 cut(s) 29
AhlI ACTAGT 1 cut(s) 301
AjnI CCWGG 5 cut(s) 316, 355, 758, 787, 814
AluBI AGCT 8 cut(s) 107, 146, 343, 512, 755, 836, 847, 971
AluI AGCT 8 cut(s) 107, 146, 343, 512, 755, 836, 847, 971
Alw26I GTCTC 2 cut(s) 459, 552
AlwI GGATC 2 cut(s) 354, 367
AoxI GGCC 4 cut(s) 34, 230, 248, 331
ApeKI GCWGC 7 cut(s) 121, 373, 590, 749, 752, 847, 1022
ApoI RAATTY 1 cut(s) 69
ArsI GACNNNNNNTTYG 2 cut(s) 272, 304
Asp700I GAANNNNTTC 1 cut(s) 24
AspS9I GGNCC 5 cut(s) 34, 332, 575, 633, 689
AvaII GGWCC 3 cut(s) 575, 633, 689
BaeI ACNNNNGTAYC 2 cut(s) 166, 199
BalI TGGCCA 1 cut(s) 232
BamHI GGATCC 1 cut(s) 359
BanII GRGCYC 1 cut(s) 875
BbrPI CACGTG 1 cut(s) 228
BbvI GCAGC 7 cut(s) 108, 360, 602, 761, 764, 834, 1034
BccI CCATC 8 cut(s) 64, 321, 476, 544, 731, 738, 956, 995
BceAI ACGGC 1 cut(s) 1010
BciT130I CCWGG 5 cut(s) 318, 357, 760, 789, 816
BcoDI GTCTC 2 cut(s) 459, 552
BcuI ACTAGT 1 cut(s) 301
BfaI CTAG 1 cut(s) 302
BfmI CTRYAG 1 cut(s) 122
BisI GCNGC 7 cut(s) 122, 374, 591, 750, 753, 848, 1023
BlsI GCNGC 7 cut(s) 123, 375, 592, 751, 754, 849, 1024
BmcAI AGTACT 1 cut(s) 300
Bme1390I CCNGG 5 cut(s) 318, 357, 760, 789, 816
Bme18I GGWCC 3 cut(s) 575, 633, 689
BmgT120I GGNCC 5 cut(s) 34, 332, 575, 633, 689
BmiI GGNNCC 4 cut(s) 82, 361, 577, 635
BmrFI CCNGG 5 cut(s) 318, 357, 760, 789, 816
BmrI ACTGGG 1 cut(s) 535
BmsI GCATC 5 cut(s) 141, 574, 666, 772, 786
BmuI ACTGGG 1 cut(s) 535
BplI GAGNNNNNCTC 2 cut(s) 321, 353
BpmI CTGGAG 1 cut(s) 492
BpuEI CTTGAG 1 cut(s) 323
BsaAI YACGTR 1 cut(s) 228
BsaI GGTCTC 1 cut(s) 552
BsaJI CCNNGG 4 cut(s) 236, 317, 758, 814
BsaWI WCCGGW 1 cut(s) 682
BsaXI ACNNNNNCTCC 2 cut(s) 928, 958
Bse1I ACTGG 4 cut(s) 530, 610, 799, 1012
Bse3DI GCAATG 1 cut(s) 994
BseBI CCWGG 5 cut(s) 318, 357, 760, 789, 816
BseDI CCNNGG 4 cut(s) 236, 317, 758, 814
BseMI GCAATG 1 cut(s) 994
BseMII CTCAG 2 cut(s) 527, 986
BseNI ACTGG 4 cut(s) 530, 610, 799, 1012
BseRI GAGGAG 3 cut(s) 774, 828, 933
BseXI GCAGC 7 cut(s) 108, 360, 602, 761, 764, 834, 1034
BseYI CCCAGC 1 cut(s) 146
BsgI GTGCAG 1 cut(s) 483
Bsh1236I CGCG 1 cut(s) 432
BshFI GGCC 4 cut(s) 36, 232, 250, 333
BsiSI CCGG 3 cut(s) 683, 711, 1080
BslFI GGGAC 3 cut(s) 21, 561, 619
BsmAI GTCTC 2 cut(s) 459, 552
BsmFI GGGAC 3 cut(s) 21, 561, 619
BsnI GGCC 4 cut(s) 36, 232, 250, 333
Bso31I GGTCTC 1 cut(s) 552
Bsp1286I GDGCHC 1 cut(s) 875
Bsp143I GATC 6 cut(s) 91, 136, 208, 359, 1042, 1072
BspACI CCGC 1 cut(s) 912
BspANI GGCC 4 cut(s) 36, 232, 250, 333
BspCNI CTCAG 2 cut(s) 526, 985
BspFNI CGCG 1 cut(s) 432
BspLI GGNNCC 4 cut(s) 82, 361, 577, 635
BspMAI CTGCAG 1 cut(s) 126
BspPI GGATC 2 cut(s) 354, 367
BspQI GCTCTTC 1 cut(s) 1012
BspTNI GGTCTC 1 cut(s) 552
BsrBI CCGCTC 1 cut(s) 912
BsrDI GCAATG 1 cut(s) 994
BsrI ACTGG 4 cut(s) 530, 610, 799, 1012
BssECI CCNNGG 4 cut(s) 236, 317, 758, 814
BssMI GATC 6 cut(s) 91, 136, 208, 359, 1042, 1072
BssT1I CCWWGG 1 cut(s) 236
Bst2UI CCWGG 5 cut(s) 318, 357, 760, 789, 816
Bst4CI ACNGT 1 cut(s) 166
Bst6I CTCTTC 2 cut(s) 10, 1012
BstBAI YACGTR 1 cut(s) 228
BstC8I GCNNGC 1 cut(s) 618
BstDEI CTNAG 2 cut(s) 513, 972
BstENI CCTNNNNNAGG 3 cut(s) 684, 813, 948
BstFNI CGCG 1 cut(s) 432
BstKTI GATC 6 cut(s) 94, 139, 211, 362, 1045, 1075
BstMAI GTCTC 2 cut(s) 459, 552
BstMBI GATC 6 cut(s) 91, 136, 208, 359, 1042, 1072
BstMWI GCNNNNNNNGC 3 cut(s) 404, 746, 984
BstNI CCWGG 5 cut(s) 318, 357, 760, 789, 816
BstNSI RCATGY 1 cut(s) 407
BstSCI CCNGG 5 cut(s) 316, 355, 758, 787, 814
BstSFI CTRYAG 1 cut(s) 122
BstUI CGCG 1 cut(s) 432
BstV1I GCAGC 7 cut(s) 108, 360, 602, 761, 764, 834, 1034
BstX2I RGATCY 1 cut(s) 359
BstYI RGATCY 1 cut(s) 359
BsuRI GGCC 4 cut(s) 36, 232, 250, 333
BtgZI GCGATG 1 cut(s) 495
BtsI GCAGTG 1 cut(s) 1018
BtsIMutI CAGTG 2 cut(s) 792, 1018
Cac8I GCNNGC 1 cut(s) 618
Cfr13I GGNCC 5 cut(s) 34, 332, 575, 633, 689
CseI GACGC 1 cut(s) 247
Csp6I GTAC 2 cut(s) 299, 434
CspCI CAANNNNNGTGG 2 cut(s) 376, 411
CviAII CATG 5 cut(s) 11, 283, 404, 980, 1060
CviQI GTAC 2 cut(s) 299, 434
DdeI CTNAG 2 cut(s) 513, 972
DpnI GATC 6 cut(s) 93, 138, 210, 361, 1044, 1074
DpnII GATC 6 cut(s) 91, 136, 208, 359, 1042, 1072
EaeI YGGCCR 1 cut(s) 230
Eam1104I CTCTTC 2 cut(s) 10, 1012
EarI CTCTTC 2 cut(s) 10, 1012
Eco130I CCWWGG 1 cut(s) 236
Eco147I AGGCCT 1 cut(s) 250
Eco24I GRGCYC 1 cut(s) 875
Eco31I GGTCTC 1 cut(s) 552
Eco47I GGWCC 3 cut(s) 575, 633, 689
Eco57I CTGAAG 1 cut(s) 81
Eco72I CACGTG 1 cut(s) 228
EcoNI CCTNNNNNAGG 3 cut(s) 684, 813, 948
EcoRII CCWGG 5 cut(s) 316, 355, 758, 787, 814
EcoT14I CCWWGG 1 cut(s) 236
EcoT38I GRGCYC 1 cut(s) 875
ErhI CCWWGG 1 cut(s) 236
FaeI CATG 5 cut(s) 14, 286, 407, 983, 1063
FaqI GGGAC 3 cut(s) 21, 561, 619
FatI CATG 5 cut(s) 10, 282, 403, 979, 1059
FauI CCCGC 1 cut(s) 905
Fnu4HI GCNGC 7 cut(s) 122, 374, 591, 750, 753, 848, 1023
FriOI GRGCYC 1 cut(s) 875
Fsp4HI GCNGC 7 cut(s) 122, 374, 591, 750, 753, 848, 1023
FspBI CTAG 1 cut(s) 302
GluI GCNGC 7 cut(s) 122, 374, 591, 750, 753, 848, 1023
GsaI CCCAGC 1 cut(s) 150
GsuI CTGGAG 1 cut(s) 492
HaeIII GGCC 4 cut(s) 36, 232, 250, 333
HapII CCGG 3 cut(s) 683, 711, 1080
HgaI GACGC 1 cut(s) 247
Hin1II CATG 5 cut(s) 14, 286, 407, 983, 1063
HincII GTYRAC 2 cut(s) 488, 533
HindII GTYRAC 2 cut(s) 488, 533
HinfI GANTC 3 cut(s) 25, 176, 886
HpaII CCGG 3 cut(s) 683, 711, 1080
Hpy166II GTNNAC 2 cut(s) 488, 533
Hpy188I TCNGA 7 cut(s) 61, 96, 310, 600, 961, 975, 1054
Hpy188III TCNNGA 3 cut(s) 78, 268, 998
Hpy8I GTNNAC 2 cut(s) 488, 533
HpyAV CCTTC 4 cut(s) 123, 240, 423, 885
HpyCH4III ACNGT 1 cut(s) 166
HpyCH4IV ACGT 2 cut(s) 227, 490
HpyCH4V TGCA 9 cut(s) 124, 154, 376, 407, 500, 590, 860, 920, 987
HpyF10VI GCNNNNNNNGC 3 cut(s) 404, 746, 984
HpyF3I CTNAG 2 cut(s) 513, 972
HpySE526I ACGT 2 cut(s) 227, 490
Hsp92II CATG 5 cut(s) 14, 286, 407, 983, 1063
Kzo9I GATC 6 cut(s) 91, 136, 208, 359, 1042, 1072
LguI GCTCTTC 1 cut(s) 1012
LmnI GCTCC 4 cut(s) 713, 760, 841, 1082
Lsp1109I GCAGC 7 cut(s) 108, 360, 602, 761, 764, 834, 1034
LweI GCATC 5 cut(s) 141, 574, 666, 772, 786
MaeI CTAG 1 cut(s) 302
MaeII ACGT 2 cut(s) 227, 490
MaeIII GTNAC 4 cut(s) 157, 278, 439, 824
MalI GATC 6 cut(s) 93, 138, 210, 361, 1044, 1074
MbiI CCGCTC 1 cut(s) 912
MboI GATC 6 cut(s) 91, 136, 208, 359, 1042, 1072
MboII GAAGA 6 cut(s) 27, 74, 1029, 1046, 1049, 1052
MfeI CAATTG 1 cut(s) 29
MflI RGATCY 1 cut(s) 359
MhlI GDGCHC 1 cut(s) 875
MlsI TGGCCA 1 cut(s) 232
MluCI AATT 3 cut(s) 29, 69, 627
MluI ACGCGT 1 cut(s) 430
MluNI TGGCCA 1 cut(s) 232
MlyI GAGTC 1 cut(s) 170
MmeI TCCRAC 3 cut(s) 623, 951, 1077
Mox20I TGGCCA 1 cut(s) 232
MroXI GAANNNNTTC 1 cut(s) 24
MscI TGGCCA 1 cut(s) 232
MseI TTAA 1 cut(s) 1029
Msp20I TGGCCA 1 cut(s) 232
MspA1I CMGCKG 1 cut(s) 847
MspI CCGG 3 cut(s) 683, 711, 1080
MspR9I CCNGG 5 cut(s) 318, 357, 760, 789, 816
MunI CAATTG 1 cut(s) 29
MvaI CCWGG 5 cut(s) 318, 357, 760, 789, 816
MvnI CGCG 1 cut(s) 432
MwoI GCNNNNNNNGC 3 cut(s) 404, 746, 984
NdeII GATC 6 cut(s) 91, 136, 208, 359, 1042, 1072
NlaIII CATG 5 cut(s) 14, 286, 407, 983, 1063
NlaIV GGNNCC 4 cut(s) 82, 361, 577, 635
NmuCI GTSAC 1 cut(s) 278
NspI RCATGY 1 cut(s) 407
PceI AGGCCT 1 cut(s) 250
PciSI GCTCTTC 1 cut(s) 1012
PdmI GAANNNNTTC 1 cut(s) 24
PfeI GAWTC 2 cut(s) 25, 886
PkrI GCNGC 7 cut(s) 123, 375, 592, 751, 754, 849, 1024
PleI GAGTC 1 cut(s) 170
PmaCI CACGTG 1 cut(s) 228
PmlI CACGTG 1 cut(s) 228
PpsI GAGTC 1 cut(s) 170
Ppu21I YACGTR 1 cut(s) 228
Psp1406I AACGTT 1 cut(s) 490
Psp6I CCWGG 5 cut(s) 316, 355, 758, 787, 814
PspCI CACGTG 1 cut(s) 228
PspFI CCCAGC 1 cut(s) 146
PspGI CCWGG 5 cut(s) 316, 355, 758, 787, 814
PspN4I GGNNCC 4 cut(s) 82, 361, 577, 635
PspPI GGNCC 5 cut(s) 34, 332, 575, 633, 689
PstI CTGCAG 1 cut(s) 126
PsuI RGATCY 1 cut(s) 359
PvuII CAGCTG 1 cut(s) 847
RsaI GTAC 2 cut(s) 300, 435
RsaNI GTAC 2 cut(s) 299, 434
SapI GCTCTTC 1 cut(s) 1012
SaqAI TTAA 1 cut(s) 1029
SatI GCNGC 7 cut(s) 122, 374, 591, 750, 753, 848, 1023
Sau3AI GATC 6 cut(s) 91, 136, 208, 359, 1042, 1072
Sau96I GGNCC 5 cut(s) 34, 332, 575, 633, 689
ScaI AGTACT 1 cut(s) 300
SchI GAGTC 1 cut(s) 170
ScrFI CCNGG 5 cut(s) 318, 357, 760, 789, 816
SduI GDGCHC 1 cut(s) 875
SfaNI GCATC 5 cut(s) 141, 574, 666, 772, 786
SfcI CTRYAG 1 cut(s) 122
SinI GGWCC 3 cut(s) 575, 633, 689
SmlI CTYRAG 1 cut(s) 338
SmoI CTYRAG 1 cut(s) 338
SpeI ACTAGT 1 cut(s) 301
Sse9I AATT 3 cut(s) 29, 69, 627
SseBI AGGCCT 1 cut(s) 250
SsiI CCGC 1 cut(s) 912
SspMI CTAG 1 cut(s) 302
StuI AGGCCT 1 cut(s) 250
StyD4I CCNGG 5 cut(s) 316, 355, 758, 787, 814
StyI CCWWGG 1 cut(s) 236
TaaI ACNGT 1 cut(s) 166
TaiI ACGT 2 cut(s) 230, 493
TaqII GACCGA 1 cut(s) 621
TasI AATT 3 cut(s) 29, 69, 627
TatI WGTACW 1 cut(s) 298
TfiI GAWTC 2 cut(s) 25, 886
Tru1I TTAA 1 cut(s) 1029
Tru9I TTAA 1 cut(s) 1029
TscAI CASTG 2 cut(s) 799, 1018
TseFI GTSAC 1 cut(s) 278
TseI GCWGC 7 cut(s) 121, 373, 590, 749, 752, 847, 1022
Tsp45I GTSAC 1 cut(s) 278
TspDTI ATGAA 1 cut(s) 81
TspGWI ACGGA 1 cut(s) 436
TspRI CASTG 2 cut(s) 799, 1018
VpaK11BI GGWCC 3 cut(s) 575, 633, 689
XagI CCTNNNNNAGG 3 cut(s) 684, 813, 948
XapI RAATTY 1 cut(s) 69
XceI RCATGY 1 cut(s) 407
XcmI CCANNNNNNNNNTGG 1 cut(s) 986
XmnI GAANNNNTTC 1 cut(s) 24
XspI CTAG 1 cut(s) 302
ZrmI AGTACT 1 cut(s) 300
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.