MD13G1016600.v1.1

transmembrane ascorbate ferrireductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0016051
Physical Location & Seq
Forward (+)
1067575 .. 1068796
1222 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1016600.v1.1.491

Sequence Viewer

Length: 684 bp
ATGCATACTACTATAAACTATAGATACGAACGATCAGCCTCTCGGTTGACAGTAGGTGCACACTTGTTTGGCATCTTAGGACTCATACTCTTGCTTGTTTGGCTCCTGCATTACCGTGGTGGCATCGACTATGATTCTGATAATTCAGATCGAGTTTTCAACGTTCATCCGTTTCTTATGTTCTTCGGGTTTATTTTTTTTGCTGGTGAAGCGATGATGGCATACAAGACCGTAATGTCGCGACACAAGGTGCAGAAGTACGTTCACGCCCTCTTACATCTGGTAGCTCTAAGTTCCGGGATATTTGGGATATGCGCTACTTTTAGATACCATGACATGAGGGACATAGATCACATGTATTCACTGCATTCATGGATTGGCTTGACCACCTTTATCTTGTACGGTTTGCAGTGGCTGTTTGGAGCTGCTACGTTCTTGTTTTCAAGAGGTATGTCAGAGTCGTCGAGGCTCAGAATAGTTCCATGGCATATGAGTATGGGTAGGGCACTGCTGTACTTGTCGATCTGTGCTGCCCTAACTGGTCTGATGGAGAAATTCACATTCCTTAGAATTTCGAATGTGCTGGGTCGCAGGGAATCACACTTGATCAATTTCACTGGACTAGCAATCCTCCTGTTTGGCATCTTCGTCGATCTCTCTGTCGCTCTTGCCCGTTATGTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0008150 GO:0008152 GO:0016491 GO:0055114
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cytochrom_B561

Protein Analysis

228

Amino Acids

26.04

Weight (kDa)

9.61

Isoelectric Point (pI)

39.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cytochrom_B561 PF03188 55 - 188 2.4e-39 Eukaryotic cytochrome b561
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0016051)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G14730
malus_domestica MD13G1016600.v1.1 MD16G1014500.v1.1
prunus_persica Prupe.1G336800_v2.0.a1
pyrus_communis pycom16g01330
rosa_chinensis RchiOBHm_Chr4g0442131
rosa_laevigata RLG00000006038
rosa_multiflora Rmu_sc0010284.1_g000013
rosa_roxburghii Rroxscaffold_5G00383040
rosa_rugosa Rorug04G0339200
rosa_samantha Rh4AG390600 Rh4BG404200 Rh4CG419200 Rh4DG397200
rosa_wichuraiana Rw4G033760

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 235
AccII CGCG 1 cut(s) 241
AclI AACGTT 1 cut(s) 162
AcsI RAATTY 2 cut(s) 554, 570
AdeI CACNNNGTG 1 cut(s) 250
AfaI GTAC 3 cut(s) 260, 401, 515
AflIII ACRYGT 1 cut(s) 354
AgsI TTSAA 2 cut(s) 160, 444
AluBI AGCT 2 cut(s) 287, 425
AluI AGCT 2 cut(s) 287, 425
Alw21I GWGCWC 1 cut(s) 61
Alw44I GTGCAC 1 cut(s) 57
AlwNI CAGNNNCTG 1 cut(s) 415
ApaLI GTGCAC 1 cut(s) 57
ApeKI GCWGC 2 cut(s) 425, 530
ApoI RAATTY 2 cut(s) 554, 570
AspLEI GCGC 1 cut(s) 317
AsuC2I CCSGG 1 cut(s) 298
AsuHPI GGTGA 1 cut(s) 218
AsuII TTCGAA 1 cut(s) 575
BaeGI GKGCMC 2 cut(s) 61, 508
Bbv12I GWGCWC 1 cut(s) 61
BbvI GCAGC 2 cut(s) 412, 517
BccI CCATC 2 cut(s) 211, 541
BcgI CGANNNNNNTGC 2 cut(s) 631, 665
BclI TGATCA 1 cut(s) 606
BcnI CCSGG 1 cut(s) 298
BfaI CTAG 1 cut(s) 623
BfmI CTRYAG 1 cut(s) 19
BisI GCNGC 2 cut(s) 426, 531
BlsI GCNGC 2 cut(s) 427, 532
Bme1390I CCNGG 1 cut(s) 298
BmiI GGNNCC 1 cut(s) 104
BmrFI CCNGG 1 cut(s) 298
BmsI GCATC 3 cut(s) 81, 132, 651
Bpu14I TTCGAA 1 cut(s) 575
BpuMI CCSGG 1 cut(s) 298
BsaJI CCNNGG 2 cut(s) 115, 482
Bse1I ACTGG 2 cut(s) 544, 622
BseDI CCNNGG 2 cut(s) 115, 482
BseGI GGATG 1 cut(s) 166
BseMII CTCAG 1 cut(s) 484
BseNI ACTGG 2 cut(s) 544, 622
BseSI GKGCMC 2 cut(s) 61, 508
BseXI GCAGC 2 cut(s) 412, 517
BseYI CCCAGC 1 cut(s) 583
BsgI GTGCAG 1 cut(s) 272
Bsh1236I CGCG 1 cut(s) 241
BsiHKAI GWGCWC 1 cut(s) 61
BsiSI CCGG 1 cut(s) 297
BslFI GGGAC 1 cut(s) 356
BsmFI GGGAC 1 cut(s) 356
BsmI GAATGC 1 cut(s) 367
Bsp119I TTCGAA 1 cut(s) 575
Bsp1286I GDGCHC 2 cut(s) 61, 508
Bsp143I GATC 6 cut(s) 32, 148, 349, 522, 606, 652
Bsp19I CCATGG 1 cut(s) 482
Bsp68I TCGCGA 1 cut(s) 241
BspCNI CTCAG 1 cut(s) 483
BspFNI CGCG 1 cut(s) 241
BspLI GGNNCC 1 cut(s) 104
BspT104I TTCGAA 1 cut(s) 575
BsrI ACTGG 2 cut(s) 544, 622
BssECI CCNNGG 2 cut(s) 115, 482
BssMI GATC 6 cut(s) 32, 148, 349, 522, 606, 652
BssT1I CCWWGG 1 cut(s) 482
Bst4CI ACNGT 4 cut(s) 52, 116, 232, 404
BstBI TTCGAA 1 cut(s) 575
BstDEI CTNAG 4 cut(s) 76, 290, 470, 566
BstDSI CCRYGG 2 cut(s) 115, 482
BstF5I GGATG 1 cut(s) 166
BstFNI CGCG 1 cut(s) 241
BstHHI GCGC 1 cut(s) 317
BstKTI GATC 6 cut(s) 35, 151, 352, 525, 609, 655
BstMBI GATC 6 cut(s) 32, 148, 349, 522, 606, 652
BstMWI GCNNNNNNNGC 3 cut(s) 100, 209, 218
BstNSI RCATGY 1 cut(s) 358
BstSCI CCNGG 1 cut(s) 296
BstSFI CTRYAG 1 cut(s) 19
BstSLI GKGCMC 2 cut(s) 61, 508
BstUI CGCG 1 cut(s) 241
BstV1I GCAGC 2 cut(s) 412, 517
BtgI CCRYGG 2 cut(s) 115, 482
BtgZI GCGATG 1 cut(s) 227
BtsCI GGATG 1 cut(s) 166
BtsI GCAGTG 3 cut(s) 362, 416, 506
BtsIMutI CAGTG 4 cut(s) 362, 416, 506, 615
BtuMI TCGCGA 1 cut(s) 241
CaiI CAGNNNCTG 1 cut(s) 415
CfoI GCGC 1 cut(s) 317
Csp6I GTAC 3 cut(s) 259, 400, 514
CviAII CATG 5 cut(s) 332, 337, 355, 372, 483
CviJI RGCY 7 cut(s) 38, 103, 287, 381, 415, 425, 469
CviKI_1 RGCY 7 cut(s) 38, 103, 287, 381, 415, 425, 469
CviQI GTAC 3 cut(s) 259, 400, 514
DdeI CTNAG 4 cut(s) 76, 290, 470, 566
DpnI GATC 6 cut(s) 34, 150, 351, 524, 608, 654
DpnII GATC 6 cut(s) 32, 148, 349, 522, 606, 652
DraIII CACNNNGTG 1 cut(s) 250
DrdI GACNNNNNNGTC 1 cut(s) 235
DseDI GACNNNNNNGTC 1 cut(s) 235
Eco130I CCWWGG 1 cut(s) 482
EcoT14I CCWWGG 1 cut(s) 482
EcoT22I ATGCAT 1 cut(s) 6
ErhI CCWWGG 1 cut(s) 482
FaeI CATG 5 cut(s) 335, 340, 358, 375, 486
FaqI GGGAC 1 cut(s) 356
FatI CATG 5 cut(s) 331, 336, 354, 371, 482
FauNDI CATATG 1 cut(s) 489
FbaI TGATCA 1 cut(s) 606
Fnu4HI GCNGC 2 cut(s) 426, 531
FokI GGATG 1 cut(s) 153
Fsp4HI GCNGC 2 cut(s) 426, 531
FspBI CTAG 1 cut(s) 623
GlaI GCGC 1 cut(s) 316
GluI GCNGC 2 cut(s) 426, 531
GsaI CCCAGC 1 cut(s) 587
HapII CCGG 1 cut(s) 297
HhaI GCGC 1 cut(s) 317
Hin1II CATG 5 cut(s) 335, 340, 358, 375, 486
Hin6I GCGC 1 cut(s) 315
HinP1I GCGC 1 cut(s) 315
HincII GTYRAC 1 cut(s) 48
HindII GTYRAC 1 cut(s) 48
HinfI GANTC 4 cut(s) 81, 134, 458, 596
HpaII CCGG 1 cut(s) 297
HphI GGTGA 1 cut(s) 218
Hpy166II GTNNAC 3 cut(s) 48, 59, 265
Hpy188I TCNGA 5 cut(s) 139, 148, 457, 473, 546
Hpy188III TCNNGA 2 cut(s) 240, 444
Hpy8I GTNNAC 3 cut(s) 48, 59, 265
Hpy99I CGWCG 2 cut(s) 466, 653
HpyCH4III ACNGT 4 cut(s) 52, 116, 232, 404
HpyCH4IV ACGT 3 cut(s) 162, 261, 431
HpyCH4V TGCA 6 cut(s) 4, 59, 109, 253, 367, 409
HpyF10VI GCNNNNNNNGC 3 cut(s) 100, 209, 218
HpyF3I CTNAG 4 cut(s) 76, 290, 470, 566
HpySE526I ACGT 3 cut(s) 162, 261, 431
Hsp92II CATG 5 cut(s) 335, 340, 358, 375, 486
HspAI GCGC 1 cut(s) 315
Ksp22I TGATCA 1 cut(s) 606
Kzo9I GATC 6 cut(s) 32, 148, 349, 522, 606, 652
LmnI GCTCC 2 cut(s) 108, 422
LpnPI CCDG 9 cut(s) 119, 189, 266, 310, 525, 569, 577, 603, 647
Lsp1109I GCAGC 2 cut(s) 412, 517
LweI GCATC 3 cut(s) 81, 132, 651
MaeI CTAG 1 cut(s) 623
MaeII ACGT 3 cut(s) 162, 261, 431
MalI GATC 6 cut(s) 34, 150, 351, 524, 608, 654
MboI GATC 6 cut(s) 32, 148, 349, 522, 606, 652
MboII GAAGA 2 cut(s) 175, 637
MhlI GDGCHC 2 cut(s) 61, 508
MluCI AATT 4 cut(s) 142, 554, 570, 610
MlyI GAGTC 2 cut(s) 75, 467
MnlI CCTC 6 cut(s) 49, 281, 333, 440, 459, 641
Mph1103I ATGCAT 1 cut(s) 6
MslI CAYNNNNRTG 1 cut(s) 114
MspI CCGG 1 cut(s) 297
MspR9I CCNGG 1 cut(s) 298
Mva1269I GAATGC 1 cut(s) 367
MvnI CGCG 1 cut(s) 241
MwoI GCNNNNNNNGC 3 cut(s) 100, 209, 218
NciI CCSGG 1 cut(s) 298
NcoI CCATGG 1 cut(s) 482
NdeI CATATG 1 cut(s) 489
NdeII GATC 6 cut(s) 32, 148, 349, 522, 606, 652
NlaIII CATG 5 cut(s) 335, 340, 358, 375, 486
NlaIV GGNNCC 1 cut(s) 104
NruI TCGCGA 1 cut(s) 241
NsiI ATGCAT 1 cut(s) 6
NspI RCATGY 1 cut(s) 358
NspV TTCGAA 1 cut(s) 575
PciI ACATGT 1 cut(s) 354
PctI GAATGC 1 cut(s) 367
PfeI GAWTC 2 cut(s) 134, 596
PfoI TCCNGGA 1 cut(s) 296
PkrI GCNGC 2 cut(s) 427, 532
PleI GAGTC 2 cut(s) 75, 466
PpsI GAGTC 2 cut(s) 75, 466
PscI ACATGT 1 cut(s) 354
Psp1406I AACGTT 1 cut(s) 162
PspFI CCCAGC 1 cut(s) 583
PspN4I GGNNCC 1 cut(s) 104
PstNI CAGNNNCTG 1 cut(s) 415
RruI TCGCGA 1 cut(s) 241
RsaI GTAC 3 cut(s) 260, 401, 515
RsaNI GTAC 3 cut(s) 259, 400, 514
RseI CAYNNNNRTG 1 cut(s) 114
SatI GCNGC 2 cut(s) 426, 531
Sau3AI GATC 6 cut(s) 32, 148, 349, 522, 606, 652
SchI GAGTC 2 cut(s) 75, 467
ScrFI CCNGG 1 cut(s) 298
SduI GDGCHC 2 cut(s) 61, 508
SetI ASST 9 cut(s) 58, 165, 252, 264, 289, 392, 427, 434, 451
SfaNI GCATC 3 cut(s) 81, 132, 651
SfcI CTRYAG 1 cut(s) 19
SfuI TTCGAA 1 cut(s) 575
SmiMI CAYNNNNRTG 1 cut(s) 114
Sse9I AATT 4 cut(s) 142, 554, 570, 610
SspMI CTAG 1 cut(s) 623
StyD4I CCNGG 1 cut(s) 296
StyI CCWWGG 1 cut(s) 482
TaaI ACNGT 4 cut(s) 52, 116, 232, 404
TaiI ACGT 3 cut(s) 165, 264, 434
TaqI TCGA 6 cut(s) 126, 151, 464, 521, 575, 651
TasI AATT 4 cut(s) 142, 554, 570, 610
TatI WGTACW 1 cut(s) 513
TfiI GAWTC 2 cut(s) 134, 596
TscAI CASTG 4 cut(s) 369, 416, 513, 622
TseI GCWGC 2 cut(s) 425, 530
TspDTI ATGAA 2 cut(s) 155, 360
TspGWI ACGGA 1 cut(s) 159
TspRI CASTG 4 cut(s) 369, 416, 513, 622
VneI GTGCAC 1 cut(s) 57
XapI RAATTY 2 cut(s) 554, 570
XceI RCATGY 1 cut(s) 358
XspI CTAG 1 cut(s) 623
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.