pycom16g01330

transmembrane ascorbate ferrireductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0016051
Physical Location & Seq
Forward (+)
848329 .. 849321
993 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom16g01330.4

Sequence Viewer

Length: 684 bp
ATGCATACTACTGTTGTAAACTATAGGTACCAACGATCAGCCTCTAGGTTGACAGTAGGTGCACACTTGTTTGGCATCTTAGCAATCATACTTATGCTTGTTTGGCTCCTGCATTACCGTGGTGGCATCGACTATGAGTCTGACAATTCAGATCGAGTTTTCAACGTTCATCCGTTTCTTATGTTCTTCGGGTTTATTTTCTTTGCTGGTGAAGCGATGATGGCATACAAGACCGTAATGGCAGAACACAAGGTGCAGAAGTACACTCACTTCTTGTTACACCTGATAGCTCTATGTACCGGGATATTTGGTATCTGCGCTGCTTTCAGATACCATGACATGAGGCAGTTAGATGACATGTATTCCCTGCATTCATGGATTGGTTTGGGTACCTTTATCTTGTACGGTTTGCAGTGGCTGTTTGGAGCTGCTGCGTTCTTGTTTCGGGGAGCTACGCCACAGTCAAGGATGAGATTAATTCCATGGCATATGAGTATGGGCAGGACAATTCTATACTTGTCGATATGTGCTGCCCTAACTGGGTTGATGCAGAAATTCACATTCCTTGGACTTTCGAAAGTGATGGGAGAGAGAGAATCGCACTTGATCAATTTCACTGCACTAGCAATCCTCCTCTTTGGCATCTTCGTCGATCTTTCTGTCGCCCTTGCCCGCTATGTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0008150 GO:0008152 GO:0016491 GO:0055114
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cytochrom_B561

Protein Analysis

228

Amino Acids

25.99

Weight (kDa)

9.35

Isoelectric Point (pI)

25.88

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0016051)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G14730
malus_domestica MD13G1016600.v1.1 MD16G1014500.v1.1
prunus_persica Prupe.1G336800_v2.0.a1
pyrus_communis pycom16g01330
rosa_chinensis RchiOBHm_Chr4g0442131
rosa_laevigata RLG00000006038
rosa_multiflora Rmu_sc0010284.1_g000013
rosa_roxburghii Rroxscaffold_5G00383040
rosa_rugosa Rorug04G0339200
rosa_samantha Rh4AG390600 Rh4BG404200 Rh4CG419200 Rh4DG397200
rosa_wichuraiana Rw4G033760

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 2 cut(s) 27, 389
AccB1I GGYRCC 2 cut(s) 27, 389
AciI CCGC 1 cut(s) 673
AclI AACGTT 1 cut(s) 165
AcsI RAATTY 1 cut(s) 554
AdeI CACNNNGTG 1 cut(s) 253
AfaI GTAC 5 cut(s) 29, 263, 298, 391, 404
AfiI CCNNNNNNNGG 1 cut(s) 540
AflIII ACRYGT 1 cut(s) 357
AgsI TTSAA 1 cut(s) 163
AhdI GACNNNNNGTC 1 cut(s) 136
AluBI AGCT 3 cut(s) 290, 428, 452
AluI AGCT 3 cut(s) 290, 428, 452
Alw21I GWGCWC 1 cut(s) 64
Alw44I GTGCAC 1 cut(s) 60
AlwNI CAGNNNCTG 1 cut(s) 418
ApaLI GTGCAC 1 cut(s) 60
ApeKI GCWGC 4 cut(s) 320, 428, 431, 530
ApoI RAATTY 1 cut(s) 554
AseI ATTAAT 1 cut(s) 476
Asp718I GGTACC 2 cut(s) 27, 389
AspLEI GCGC 1 cut(s) 320
AsuC2I CCSGG 1 cut(s) 301
AsuHPI GGTGA 1 cut(s) 221
AsuII TTCGAA 1 cut(s) 575
BaeGI GKGCMC 1 cut(s) 64
BanI GGYRCC 2 cut(s) 27, 389
BarI GAAGNNNNNNTAC 2 cut(s) 254, 286
Bbv12I GWGCWC 1 cut(s) 64
BbvI GCAGC 4 cut(s) 307, 415, 418, 517
BccI CCATC 2 cut(s) 214, 577
BcgI CGANNNNNNTGC 2 cut(s) 631, 665
BclI TGATCA 1 cut(s) 606
BcnI CCSGG 1 cut(s) 301
BfaI CTAG 2 cut(s) 45, 623
BfmI CTRYAG 1 cut(s) 22
BisI GCNGC 4 cut(s) 321, 429, 432, 531
BlsI GCNGC 4 cut(s) 322, 430, 433, 532
Bme1390I CCNGG 1 cut(s) 301
BmeRI GACNNNNNGTC 1 cut(s) 136
BmiI GGNNCC 3 cut(s) 29, 107, 391
BmrFI CCNGG 1 cut(s) 301
BmrI ACTGGG 1 cut(s) 549
BmsI GCATC 4 cut(s) 84, 135, 537, 651
BmuI ACTGGG 1 cut(s) 549
Bpu14I TTCGAA 1 cut(s) 575
BpuMI CCSGG 1 cut(s) 301
BsaJI CCNNGG 3 cut(s) 118, 482, 565
Bsc4I CCNNNNNNNGG 1 cut(s) 540
Bse1I ACTGG 1 cut(s) 544
BseDI CCNNGG 3 cut(s) 118, 482, 565
BseGI GGATG 2 cut(s) 169, 474
BseLI CCNNNNNNNGG 1 cut(s) 540
BseNI ACTGG 1 cut(s) 544
BseRI GAGGAG 1 cut(s) 623
BseSI GKGCMC 1 cut(s) 64
BseXI GCAGC 4 cut(s) 307, 415, 418, 517
BsgI GTGCAG 2 cut(s) 275, 603
BshNI GGYRCC 2 cut(s) 27, 389
BsiHKAI GWGCWC 1 cut(s) 64
BsiSI CCGG 1 cut(s) 300
BslI CCNNNNNNNGG 1 cut(s) 540
BsmI GAATGC 1 cut(s) 370
Bsp119I TTCGAA 1 cut(s) 575
Bsp1286I GDGCHC 1 cut(s) 64
Bsp143I GATC 4 cut(s) 35, 151, 606, 652
Bsp19I CCATGG 1 cut(s) 482
BspACI CCGC 1 cut(s) 673
BspLI GGNNCC 3 cut(s) 29, 107, 391
BspT104I TTCGAA 1 cut(s) 575
BspT107I GGYRCC 2 cut(s) 27, 389
BsrI ACTGG 1 cut(s) 544
BssECI CCNNGG 3 cut(s) 118, 482, 565
BssMI GATC 4 cut(s) 35, 151, 606, 652
BssT1I CCWWGG 2 cut(s) 482, 565
Bst4CI ACNGT 6 cut(s) 13, 55, 119, 235, 407, 462
BstBI TTCGAA 1 cut(s) 575
BstC8I GCNNGC 1 cut(s) 673
BstDEI CTNAG 1 cut(s) 79
BstDSI CCRYGG 2 cut(s) 118, 482
BstF5I GGATG 2 cut(s) 169, 474
BstHHI GCGC 1 cut(s) 320
BstKTI GATC 4 cut(s) 38, 154, 609, 655
BstMBI GATC 4 cut(s) 35, 151, 606, 652
BstMWI GCNNNNNNNGC 3 cut(s) 103, 212, 221
BstNSI RCATGY 1 cut(s) 361
BstSCI CCNGG 1 cut(s) 299
BstSFI CTRYAG 1 cut(s) 22
BstSLI GKGCMC 1 cut(s) 64
BstV1I GCAGC 4 cut(s) 307, 415, 418, 517
BtgI CCRYGG 2 cut(s) 118, 482
BtgZI GCGATG 1 cut(s) 230
BtsCI GGATG 2 cut(s) 169, 474
BtsI GCAGTG 2 cut(s) 419, 615
BtsIMutI CAGTG 2 cut(s) 419, 615
Cac8I GCNNGC 1 cut(s) 673
CaiI CAGNNNCTG 1 cut(s) 418
CfoI GCGC 1 cut(s) 320
Csp6I GTAC 5 cut(s) 28, 262, 297, 390, 403
CviAII CATG 5 cut(s) 335, 340, 358, 375, 483
CviJI RGCY 6 cut(s) 41, 106, 290, 418, 428, 452
CviKI_1 RGCY 6 cut(s) 41, 106, 290, 418, 428, 452
CviQI GTAC 5 cut(s) 28, 262, 297, 390, 403
DdeI CTNAG 1 cut(s) 79
DpnI GATC 4 cut(s) 37, 153, 608, 654
DpnII GATC 4 cut(s) 35, 151, 606, 652
DraIII CACNNNGTG 1 cut(s) 253
DriI GACNNNNNGTC 1 cut(s) 136
Eam1105I GACNNNNNGTC 1 cut(s) 136
Eco130I CCWWGG 2 cut(s) 482, 565
EcoT14I CCWWGG 2 cut(s) 482, 565
EcoT22I ATGCAT 1 cut(s) 6
ErhI CCWWGG 2 cut(s) 482, 565
FaeI CATG 5 cut(s) 338, 343, 361, 378, 486
FatI CATG 5 cut(s) 334, 339, 357, 374, 482
FauI CCCGC 1 cut(s) 680
FauNDI CATATG 1 cut(s) 489
FbaI TGATCA 1 cut(s) 606
Fnu4HI GCNGC 4 cut(s) 321, 429, 432, 531
FokI GGATG 2 cut(s) 156, 481
Fsp4HI GCNGC 4 cut(s) 321, 429, 432, 531
FspBI CTAG 2 cut(s) 45, 623
GlaI GCGC 1 cut(s) 319
GluI GCNGC 4 cut(s) 321, 429, 432, 531
HapII CCGG 1 cut(s) 300
HhaI GCGC 1 cut(s) 320
Hin1II CATG 5 cut(s) 338, 343, 361, 378, 486
Hin6I GCGC 1 cut(s) 318
HinP1I GCGC 1 cut(s) 318
HincII GTYRAC 1 cut(s) 51
HindII GTYRAC 1 cut(s) 51
HinfI GANTC 2 cut(s) 137, 596
HpaII CCGG 1 cut(s) 300
HphI GGTGA 1 cut(s) 221
Hpy166II GTNNAC 4 cut(s) 19, 51, 62, 264
Hpy188I TCNGA 3 cut(s) 142, 151, 329
Hpy8I GTNNAC 4 cut(s) 19, 51, 62, 264
Hpy99I CGWCG 1 cut(s) 653
HpyCH4III ACNGT 6 cut(s) 13, 55, 119, 235, 407, 462
HpyCH4IV ACGT 1 cut(s) 165
HpyCH4V TGCA 8 cut(s) 4, 62, 112, 256, 370, 412, 550, 620
HpyF10VI GCNNNNNNNGC 3 cut(s) 103, 212, 221
HpyF3I CTNAG 1 cut(s) 79
HpySE526I ACGT 1 cut(s) 165
Hsp92II CATG 5 cut(s) 338, 343, 361, 378, 486
HspAI GCGC 1 cut(s) 318
KpnI GGTACC 2 cut(s) 31, 393
Ksp22I TGATCA 1 cut(s) 606
Kzo9I GATC 4 cut(s) 35, 151, 606, 652
LmnI GCTCC 3 cut(s) 111, 425, 449
LpnPI CCDG 7 cut(s) 122, 192, 296, 313, 380, 487, 525
Lsp1109I GCAGC 4 cut(s) 307, 415, 418, 517
LweI GCATC 4 cut(s) 84, 135, 537, 651
MaeI CTAG 2 cut(s) 45, 623
MaeII ACGT 1 cut(s) 165
MaeIII GTNAC 1 cut(s) 276
MalI GATC 4 cut(s) 37, 153, 608, 654
MboI GATC 4 cut(s) 35, 151, 606, 652
MboII GAAGA 2 cut(s) 178, 637
MhlI GDGCHC 1 cut(s) 64
MluCI AATT 5 cut(s) 145, 477, 507, 554, 610
MlyI GAGTC 1 cut(s) 146
MnlI CCTC 4 cut(s) 52, 336, 641, 644
Mph1103I ATGCAT 1 cut(s) 6
MseI TTAA 1 cut(s) 476
MslI CAYNNNNRTG 2 cut(s) 92, 117
MspI CCGG 1 cut(s) 300
MspR9I CCNGG 1 cut(s) 301
Mva1269I GAATGC 1 cut(s) 370
MwoI GCNNNNNNNGC 3 cut(s) 103, 212, 221
NciI CCSGG 1 cut(s) 301
NcoI CCATGG 1 cut(s) 482
NdeI CATATG 1 cut(s) 489
NdeII GATC 4 cut(s) 35, 151, 606, 652
NlaIII CATG 5 cut(s) 338, 343, 361, 378, 486
NlaIV GGNNCC 3 cut(s) 29, 107, 391
NsiI ATGCAT 1 cut(s) 6
NspI RCATGY 1 cut(s) 361
NspV TTCGAA 1 cut(s) 575
PciI ACATGT 1 cut(s) 357
PctI GAATGC 1 cut(s) 370
PfeI GAWTC 1 cut(s) 596
PkrI GCNGC 4 cut(s) 322, 430, 433, 532
PleI GAGTC 1 cut(s) 145
PpsI GAGTC 1 cut(s) 145
PscI ACATGT 1 cut(s) 357
PshBI ATTAAT 1 cut(s) 476
Psp1406I AACGTT 1 cut(s) 165
PspN4I GGNNCC 3 cut(s) 29, 107, 391
PstNI CAGNNNCTG 1 cut(s) 418
RsaI GTAC 5 cut(s) 29, 263, 298, 391, 404
RsaNI GTAC 5 cut(s) 28, 262, 297, 390, 403
RseI CAYNNNNRTG 2 cut(s) 92, 117
SaqAI TTAA 1 cut(s) 476
SatI GCNGC 4 cut(s) 321, 429, 432, 531
Sau3AI GATC 4 cut(s) 35, 151, 606, 652
SchI GAGTC 1 cut(s) 146
ScrFI CCNGG 1 cut(s) 301
SduI GDGCHC 1 cut(s) 64
SfaNI GCATC 4 cut(s) 84, 135, 537, 651
SfcI CTRYAG 1 cut(s) 22
SfuI TTCGAA 1 cut(s) 575
SmiMI CAYNNNNRTG 2 cut(s) 92, 117
Sse9I AATT 5 cut(s) 145, 477, 507, 554, 610
SsiI CCGC 1 cut(s) 673
SspMI CTAG 2 cut(s) 45, 623
StyD4I CCNGG 1 cut(s) 299
StyI CCWWGG 2 cut(s) 482, 565
TaaI ACNGT 6 cut(s) 13, 55, 119, 235, 407, 462
TaiI ACGT 1 cut(s) 168
TaqI TCGA 5 cut(s) 129, 154, 521, 575, 651
TasI AATT 5 cut(s) 145, 477, 507, 554, 610
TatI WGTACW 1 cut(s) 261
TfiI GAWTC 1 cut(s) 596
Tru1I TTAA 1 cut(s) 476
Tru9I TTAA 1 cut(s) 476
TscAI CASTG 2 cut(s) 419, 622
TseI GCWGC 4 cut(s) 320, 428, 431, 530
TspDTI ATGAA 2 cut(s) 158, 363
TspGWI ACGGA 1 cut(s) 162
TspRI CASTG 2 cut(s) 419, 622
VneI GTGCAC 1 cut(s) 60
VspI ATTAAT 1 cut(s) 476
XapI RAATTY 1 cut(s) 554
XceI RCATGY 1 cut(s) 361
XspI CTAG 2 cut(s) 45, 623
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.