MD16G1014500.v1.1

transmembrane ascorbate ferrireductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0016051
Physical Location & Seq
Forward (+)
1126066 .. 1127540
1475 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1014500.v1.1.491

Sequence Viewer

Length: 864 bp
ATGGGCATTTTTTATCACCAACTCTTACAAAGATACATATATATAAGAACTCATTCAGCAGAACGCTCCCACATCAAAATTAAAGGAACCAAATTTAAGATATTAACCCACCAATCCCCATCCTTAATTCCCCAGATTAATTCACTTCGCCATCATCTGTCGTCTAATCACTTAATCGCAATGCATACTACTGTTGTAAACTATAGGTACAAACGATCAGCCTCTAGGTTGACAGTAGGTGCACACTTGTTTGGCATCTTAGCAATCATACTTATGCTTGTTTGGCTCCTGCATTACCGTGGTGGCATCGATTATGATTCTGACAATTCAGATCGAGTTTTCAACGTTCATCCGTTTCTTATGTTCTTCGGTTTTATTTTCTTTGCTGGTGAAGCGATGATGGCATACAAGACCGTAATGTCAGAACACCAGGGGCAGAAGTATATGCACGCCTTGTTACACCTGATAGCTCTATGTACCGGAATATTTGGGATATGCGCTGCTTTTAGATACCATGACATGAGGCAGATAGATGACATGTATACCCTGCATTCATGGATTGGCTTGGGCACCTTTATCTTGTACGGTTTGCAGTGGCTGGTTGGAGCTGCTGCTTTCTTGTTTCCAGGAGGTACGGCAGAGTCGAGGTTGAGATTAATTCCATGGCATATGAGTATGGGCAGGACAATTCTATACTTGTCGATATGTGCTGCCCTAACTGGGTTGATGCAGAAATTCACATTCCTTGGACTTTCGAAAGCGCTGGGAGACAGAGAATCGCACTTGATCAATTTCACTGCACTAGCAATCCTCCTCTTTGGCATCTTCGTCGATCTTTCTGTCGCCCTTGCCCGTTATGTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0008150 GO:0008152 GO:0016491 GO:0055114
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cytochrom_B561

Protein Analysis

288

Amino Acids

32.75

Weight (kDa)

9.68

Isoelectric Point (pI)

40.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cytochrom_B561 PF03188 116 - 248 4.6e-39 Eukaryotic cytochrome b561
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0016051)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G14730
malus_domestica MD13G1016600.v1.1 MD16G1014500.v1.1
prunus_persica Prupe.1G336800_v2.0.a1
pyrus_communis pycom16g01330
rosa_chinensis RchiOBHm_Chr4g0442131
rosa_laevigata RLG00000006038
rosa_multiflora Rmu_sc0010284.1_g000013
rosa_roxburghii Rroxscaffold_5G00383040
rosa_rugosa Rorug04G0339200
rosa_samantha Rh4AG390600 Rh4BG404200 Rh4CG419200 Rh4DG397200
rosa_wichuraiana Rw4G033760

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 418
AccB1I GGYRCC 1 cut(s) 569
AccI GTMKAC 1 cut(s) 542
AclI AACGTT 1 cut(s) 345
AcsI RAATTY 2 cut(s) 92, 734
AfaI GTAC 4 cut(s) 209, 478, 584, 634
AfeI AGCGCT 1 cut(s) 762
AfiI CCNNNNNNNGG 1 cut(s) 720
AflIII ACRYGT 1 cut(s) 537
AgsI TTSAA 1 cut(s) 343
AjnI CCWGG 2 cut(s) 429, 625
AluBI AGCT 2 cut(s) 470, 608
AluI AGCT 2 cut(s) 470, 608
Alw21I GWGCWC 1 cut(s) 244
Alw26I GTCTC 1 cut(s) 762
Alw44I GTGCAC 1 cut(s) 240
AlwNI CAGNNNCTG 1 cut(s) 598
Aor51HI AGCGCT 1 cut(s) 762
ApaLI GTGCAC 1 cut(s) 240
ApeKI GCWGC 4 cut(s) 500, 608, 611, 710
ApoI RAATTY 2 cut(s) 92, 734
AseI ATTAAT 2 cut(s) 138, 656
Asp700I GAANNNNTTC 1 cut(s) 52
AspLEI GCGC 2 cut(s) 500, 763
AsuHPI GGTGA 2 cut(s) 8, 401
AsuII TTCGAA 1 cut(s) 755
BaeGI GKGCMC 2 cut(s) 244, 572
BanI GGYRCC 1 cut(s) 569
Bbv12I GWGCWC 1 cut(s) 244
BbvI GCAGC 4 cut(s) 487, 595, 598, 697
BccI CCATC 3 cut(s) 127, 159, 394
BceAI ACGGC 1 cut(s) 651
BcgI CGANNNNNNTGC 2 cut(s) 811, 845
BciT130I CCWGG 2 cut(s) 431, 627
BclI TGATCA 1 cut(s) 786
BcoDI GTCTC 1 cut(s) 762
BfaI CTAG 2 cut(s) 225, 803
BfmI CTRYAG 1 cut(s) 202
BfoI RGCGCY 1 cut(s) 764
BisI GCNGC 4 cut(s) 501, 609, 612, 711
BlsI GCNGC 4 cut(s) 502, 610, 613, 712
Bme1390I CCNGG 2 cut(s) 431, 627
BmiI GGNNCC 3 cut(s) 88, 287, 571
BmrFI CCNGG 2 cut(s) 431, 627
BmrI ACTGGG 1 cut(s) 729
BmsI GCATC 4 cut(s) 264, 315, 717, 831
BmuI ACTGGG 1 cut(s) 729
Bpu14I TTCGAA 1 cut(s) 755
Bsa29I ATCGAT 1 cut(s) 309
BsaJI CCNNGG 4 cut(s) 298, 430, 662, 745
BsaWI WCCGGW 1 cut(s) 479
Bsc4I CCNNNNNNNGG 1 cut(s) 720
Bse1I ACTGG 1 cut(s) 724
Bse3DI GCAATG 1 cut(s) 186
BseBI CCWGG 2 cut(s) 431, 627
BseCI ATCGAT 1 cut(s) 309
BseDI CCNNGG 4 cut(s) 298, 430, 662, 745
BseGI GGATG 2 cut(s) 119, 349
BseLI CCNNNNNNNGG 1 cut(s) 720
BseMI GCAATG 1 cut(s) 186
BseNI ACTGG 1 cut(s) 724
BseRI GAGGAG 1 cut(s) 803
BseSI GKGCMC 2 cut(s) 244, 572
BseXI GCAGC 4 cut(s) 487, 595, 598, 697
BseYI CCCAGC 1 cut(s) 763
BsgI GTGCAG 1 cut(s) 783
BshNI GGYRCC 1 cut(s) 569
BshVI ATCGAT 1 cut(s) 309
BsiHKAI GWGCWC 1 cut(s) 244
BsiSI CCGG 1 cut(s) 480
BslI CCNNNNNNNGG 1 cut(s) 720
BsmAI GTCTC 1 cut(s) 762
BsmI GAATGC 1 cut(s) 550
Bsp119I TTCGAA 1 cut(s) 755
Bsp1286I GDGCHC 2 cut(s) 244, 572
Bsp143I GATC 4 cut(s) 215, 331, 786, 832
Bsp19I CCATGG 1 cut(s) 662
BspDI ATCGAT 1 cut(s) 309
BspLI GGNNCC 3 cut(s) 88, 287, 571
BspT104I TTCGAA 1 cut(s) 755
BspT107I GGYRCC 1 cut(s) 569
BsrDI GCAATG 1 cut(s) 186
BsrI ACTGG 1 cut(s) 724
BssECI CCNNGG 4 cut(s) 298, 430, 662, 745
BssMI GATC 4 cut(s) 215, 331, 786, 832
BssNAI GTATAC 1 cut(s) 543
BssT1I CCWWGG 2 cut(s) 662, 745
Bst1107I GTATAC 1 cut(s) 543
Bst2UI CCWGG 2 cut(s) 431, 627
Bst4CI ACNGT 5 cut(s) 193, 235, 299, 415, 587
BstBI TTCGAA 1 cut(s) 755
BstC8I GCNNGC 1 cut(s) 450
BstDEI CTNAG 1 cut(s) 259
BstDSI CCRYGG 2 cut(s) 298, 662
BstF5I GGATG 2 cut(s) 119, 349
BstH2I RGCGCY 1 cut(s) 764
BstHHI GCGC 2 cut(s) 500, 763
BstKTI GATC 4 cut(s) 218, 334, 789, 835
BstMAI GTCTC 1 cut(s) 762
BstMBI GATC 4 cut(s) 215, 331, 786, 832
BstMWI GCNNNNNNNGC 3 cut(s) 283, 392, 401
BstNI CCWGG 2 cut(s) 431, 627
BstNSI RCATGY 1 cut(s) 541
BstSCI CCNGG 2 cut(s) 429, 625
BstSFI CTRYAG 1 cut(s) 202
BstSLI GKGCMC 2 cut(s) 244, 572
BstV1I GCAGC 4 cut(s) 487, 595, 598, 697
BstZ17I GTATAC 1 cut(s) 543
Bsu15I ATCGAT 1 cut(s) 309
BsuTUI ATCGAT 1 cut(s) 309
BtgI CCRYGG 2 cut(s) 298, 662
BtgZI GCGATG 1 cut(s) 410
BtsCI GGATG 2 cut(s) 119, 349
BtsI GCAGTG 2 cut(s) 599, 795
BtsIMutI CAGTG 2 cut(s) 599, 795
Cac8I GCNNGC 1 cut(s) 450
CaiI CAGNNNCTG 1 cut(s) 598
CfoI GCGC 2 cut(s) 500, 763
ClaI ATCGAT 1 cut(s) 309
Csp6I GTAC 4 cut(s) 208, 477, 583, 633
CviAII CATG 5 cut(s) 515, 520, 538, 555, 663
CviJI RGCY 6 cut(s) 221, 286, 470, 564, 598, 608
CviKI_1 RGCY 6 cut(s) 221, 286, 470, 564, 598, 608
CviQI GTAC 4 cut(s) 208, 477, 583, 633
DdeI CTNAG 1 cut(s) 259
DpnI GATC 4 cut(s) 217, 333, 788, 834
DpnII GATC 4 cut(s) 215, 331, 786, 832
DrdI GACNNNNNNGTC 1 cut(s) 418
DseDI GACNNNNNNGTC 1 cut(s) 418
Eco130I CCWWGG 2 cut(s) 662, 745
Eco47III AGCGCT 1 cut(s) 762
EcoRII CCWGG 2 cut(s) 429, 625
EcoT14I CCWWGG 2 cut(s) 662, 745
EcoT22I ATGCAT 1 cut(s) 186
ErhI CCWWGG 2 cut(s) 662, 745
FaeI CATG 5 cut(s) 518, 523, 541, 558, 666
FatI CATG 5 cut(s) 514, 519, 537, 554, 662
FauNDI CATATG 1 cut(s) 669
FbaI TGATCA 1 cut(s) 786
FblI GTMKAC 1 cut(s) 542
Fnu4HI GCNGC 4 cut(s) 501, 609, 612, 711
FokI GGATG 2 cut(s) 106, 336
Fsp4HI GCNGC 4 cut(s) 501, 609, 612, 711
FspBI CTAG 2 cut(s) 225, 803
GlaI GCGC 2 cut(s) 499, 762
GluI GCNGC 4 cut(s) 501, 609, 612, 711
GsaI CCCAGC 1 cut(s) 767
HaeII RGCGCY 1 cut(s) 764
HapII CCGG 1 cut(s) 480
HhaI GCGC 2 cut(s) 500, 763
Hin1II CATG 5 cut(s) 518, 523, 541, 558, 666
Hin6I GCGC 2 cut(s) 498, 761
HinP1I GCGC 2 cut(s) 498, 761
HincII GTYRAC 1 cut(s) 231
HindII GTYRAC 1 cut(s) 231
HinfI GANTC 3 cut(s) 317, 641, 776
HpaII CCGG 1 cut(s) 480
HphI GGTGA 2 cut(s) 8, 401
Hpy166II GTNNAC 4 cut(s) 199, 231, 242, 543
Hpy188I TCNGA 3 cut(s) 322, 331, 424
Hpy8I GTNNAC 4 cut(s) 199, 231, 242, 543
Hpy99I CGWCG 1 cut(s) 833
HpyCH4III ACNGT 5 cut(s) 193, 235, 299, 415, 587
HpyCH4IV ACGT 1 cut(s) 345
HpyCH4V TGCA 8 cut(s) 184, 242, 292, 448, 550, 592, 730, 800
HpyF10VI GCNNNNNNNGC 3 cut(s) 283, 392, 401
HpyF3I CTNAG 1 cut(s) 259
HpySE526I ACGT 1 cut(s) 345
Hsp92II CATG 5 cut(s) 518, 523, 541, 558, 666
HspAI GCGC 2 cut(s) 498, 761
Ksp22I TGATCA 1 cut(s) 786
Kzo9I GATC 4 cut(s) 215, 331, 786, 832
LmnI GCTCC 3 cut(s) 71, 291, 605
Lsp1109I GCAGC 4 cut(s) 487, 595, 598, 697
LweI GCATC 4 cut(s) 264, 315, 717, 831
MaeI CTAG 2 cut(s) 225, 803
MaeII ACGT 1 cut(s) 345
MaeIII GTNAC 1 cut(s) 456
MalI GATC 4 cut(s) 217, 333, 788, 834
MboI GATC 4 cut(s) 215, 331, 786, 832
MboII GAAGA 2 cut(s) 358, 817
MhlI GDGCHC 2 cut(s) 244, 572
MluCI AATT 9 cut(s) 78, 92, 126, 139, 325, 657, 687, 734, 790
MlyI GAGTC 1 cut(s) 650
MmeI TCCRAC 1 cut(s) 583
MnlI CCTC 6 cut(s) 232, 516, 623, 639, 821, 824
Mph1103I ATGCAT 1 cut(s) 186
MroXI GAANNNNTTC 1 cut(s) 52
MseI TTAA 7 cut(s) 81, 96, 104, 125, 138, 173, 656
MslI CAYNNNNRTG 2 cut(s) 272, 297
MspI CCGG 1 cut(s) 480
MspR9I CCNGG 2 cut(s) 431, 627
Mva1269I GAATGC 1 cut(s) 550
MvaI CCWGG 2 cut(s) 431, 627
MwoI GCNNNNNNNGC 3 cut(s) 283, 392, 401
NcoI CCATGG 1 cut(s) 662
NdeI CATATG 1 cut(s) 669
NdeII GATC 4 cut(s) 215, 331, 786, 832
NlaIII CATG 5 cut(s) 518, 523, 541, 558, 666
NlaIV GGNNCC 3 cut(s) 88, 287, 571
NsiI ATGCAT 1 cut(s) 186
NspI RCATGY 1 cut(s) 541
NspV TTCGAA 1 cut(s) 755
PciI ACATGT 1 cut(s) 537
PcsI WCGNNNNNNNCGW 1 cut(s) 641
PctI GAATGC 1 cut(s) 550
PdmI GAANNNNTTC 1 cut(s) 52
PfeI GAWTC 2 cut(s) 317, 776
PfoI TCCNGGA 1 cut(s) 625
PkrI GCNGC 4 cut(s) 502, 610, 613, 712
PleI GAGTC 1 cut(s) 649
PpsI GAGTC 1 cut(s) 649
PscI ACATGT 1 cut(s) 537
PshBI ATTAAT 2 cut(s) 138, 656
Psp1406I AACGTT 1 cut(s) 345
Psp6I CCWGG 2 cut(s) 429, 625
PspFI CCCAGC 1 cut(s) 763
PspGI CCWGG 2 cut(s) 429, 625
PspN4I GGNNCC 3 cut(s) 88, 287, 571
PstNI CAGNNNCTG 1 cut(s) 598
RsaI GTAC 4 cut(s) 209, 478, 584, 634
RsaNI GTAC 4 cut(s) 208, 477, 583, 633
RseI CAYNNNNRTG 2 cut(s) 272, 297
SaqAI TTAA 7 cut(s) 81, 96, 104, 125, 138, 173, 656
SatI GCNGC 4 cut(s) 501, 609, 612, 711
Sau3AI GATC 4 cut(s) 215, 331, 786, 832
SchI GAGTC 1 cut(s) 650
ScrFI CCNGG 2 cut(s) 431, 627
SduI GDGCHC 2 cut(s) 244, 572
SfaNI GCATC 4 cut(s) 264, 315, 717, 831
SfcI CTRYAG 1 cut(s) 202
SfuI TTCGAA 1 cut(s) 755
SmiMI CAYNNNNRTG 2 cut(s) 272, 297
Sse9I AATT 9 cut(s) 78, 92, 126, 139, 325, 657, 687, 734, 790
SspI AATATT 1 cut(s) 486
SspMI CTAG 2 cut(s) 225, 803
StyD4I CCNGG 2 cut(s) 429, 625
StyI CCWWGG 2 cut(s) 662, 745
TaaI ACNGT 5 cut(s) 193, 235, 299, 415, 587
TaiI ACGT 1 cut(s) 348
TaqI TCGA 6 cut(s) 309, 334, 644, 701, 755, 831
TasI AATT 9 cut(s) 78, 92, 126, 139, 325, 657, 687, 734, 790
TfiI GAWTC 2 cut(s) 317, 776
Tru1I TTAA 7 cut(s) 81, 96, 104, 125, 138, 173, 656
Tru9I TTAA 7 cut(s) 81, 96, 104, 125, 138, 173, 656
TscAI CASTG 2 cut(s) 599, 802
TseI GCWGC 4 cut(s) 500, 608, 611, 710
TspDTI ATGAA 2 cut(s) 338, 543
TspGWI ACGGA 1 cut(s) 342
TspRI CASTG 2 cut(s) 599, 802
VneI GTGCAC 1 cut(s) 240
VspI ATTAAT 2 cut(s) 138, 656
XapI RAATTY 2 cut(s) 92, 734
XceI RCATGY 1 cut(s) 541
XmiI GTMKAC 1 cut(s) 542
XmnI GAANNNNTTC 1 cut(s) 52
XspI CTAG 2 cut(s) 225, 803
Zsp2I ATGCAT 1 cut(s) 186
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.