Rh4BG404200

transmembrane ascorbate ferrireductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0016051
Physical Location & Seq
Forward (+)
55473534 .. 55475148
1615 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG404200.1

Sequence Viewer

Length: 501 bp
ATGTTCTTCGGATTCATTTTCTTTGCTGGTGAAGCAATGATGGCATACAAGACTGTAAAGTCGCAACACAACGTGCAGAAGTACGTTCACGGCTTCTTTCACCTGGTAGCTCTTTGTTCCGGAATATTCGGAATATGTGCTGTTTTCAGGTTCCACGATATGGCGGGAAATCTAGAGGACATGTATAGCCTCCATTCATGGATTGGCTTCACCACAATTATCTTGTTCGGCTTGCAGTGGTTGTTTGGATTTGCTACATTCTTGTTTCCAAGAGCGTCAGAACAGTCGAGGCTCCAAATTGCTCCTTGGCATATGAGTTTCGGCAGGGTGCTCCTATACATGTCAATATGTGCCGCCATGTCCGGCTTGATGGAGAAATCCACATTCCTCCGGGATAAGATAAGTGAAAGAGAATTACATCTCATCAACTTCACTGGACTCTCAATCCTCCTCTTTGGCATCTTCGTTGATCTTTCAGTTGCTCTGGCGCGTTACGTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0008150 GO:0008152 GO:0016491 GO:0055114
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cytochrom_B561

Protein Analysis

166

Amino Acids

19.0

Weight (kDa)

8.51

Isoelectric Point (pI)

39.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cytochrom_B561 PF03188 1 - 129 9e-38 Eukaryotic cytochrome b561
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016051)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G14730
malus_domestica MD13G1016600.v1.1 MD16G1014500.v1.1
prunus_persica Prupe.1G336800_v2.0.a1
pyrus_communis pycom16g01330
rosa_chinensis RchiOBHm_Chr4g0442131
rosa_laevigata RLG00000006038
rosa_multiflora Rmu_sc0010284.1_g000013
rosa_roxburghii Rroxscaffold_5G00383040
rosa_rugosa Rorug04G0339200
rosa_samantha Rh4AG390600 Rh4BG404200 Rh4CG419200 Rh4DG397200
rosa_wichuraiana Rw4G033760

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 58
AccB7I CCANNNNNTGG 1 cut(s) 160
AccII CGCG 1 cut(s) 490
AccIII TCCGGA 1 cut(s) 119
AciI CCGC 2 cut(s) 164, 354
AdeI CACNNNGTG 1 cut(s) 73
AfaI GTAC 1 cut(s) 83
AfiI CCNNNNNNNGG 1 cut(s) 160
AflIII ACRYGT 3 cut(s) 180, 339, 495
AjnI CCWGG 1 cut(s) 102
AluBI AGCT 1 cut(s) 110
AluI AGCT 1 cut(s) 110
Alw21I GWGCWC 1 cut(s) 333
Aor13HI TCCGGA 1 cut(s) 119
AspLEI GCGC 1 cut(s) 490
AsuC2I CCSGG 1 cut(s) 392
AsuHPI GGTGA 3 cut(s) 41, 92, 202
Bbv12I GWGCWC 1 cut(s) 333
BccI CCATC 2 cut(s) 34, 364
BceAI ACGGC 1 cut(s) 106
BciT130I CCWGG 1 cut(s) 104
BcnI CCSGG 1 cut(s) 392
BfaI CTAG 1 cut(s) 173
BisI GCNGC 1 cut(s) 354
BlsI GCNGC 1 cut(s) 355
Bme1390I CCNGG 2 cut(s) 104, 392
BmiI GGNNCC 2 cut(s) 152, 293
BmrFI CCNGG 2 cut(s) 104, 392
BmsI GCATC 1 cut(s) 468
BpuMI CCSGG 1 cut(s) 392
BsaAI YACGTR 1 cut(s) 496
BsaJI CCNNGG 1 cut(s) 305
BsaWI WCCGGW 1 cut(s) 119
Bsc4I CCNNNNNNNGG 1 cut(s) 160
Bse1I ACTGG 1 cut(s) 439
Bse3DI GCAATG 1 cut(s) 42
BseAI TCCGGA 1 cut(s) 119
BseBI CCWGG 1 cut(s) 104
BseDI CCNNGG 1 cut(s) 305
BseLI CCNNNNNNNGG 1 cut(s) 160
BseMI GCAATG 1 cut(s) 42
BseNI ACTGG 1 cut(s) 439
BseRI GAGGAG 1 cut(s) 440
BsgI GTGCAG 1 cut(s) 95
Bsh1236I CGCG 1 cut(s) 490
BsiHKAI GWGCWC 1 cut(s) 333
BsiSI CCGG 3 cut(s) 120, 363, 391
BslI CCNNNNNNNGG 1 cut(s) 160
Bsp1286I GDGCHC 1 cut(s) 333
Bsp13I TCCGGA 1 cut(s) 119
Bsp143I GATC 1 cut(s) 469
BspACI CCGC 2 cut(s) 164, 354
BspEI TCCGGA 1 cut(s) 119
BspFNI CGCG 1 cut(s) 490
BspLI GGNNCC 2 cut(s) 152, 293
BsrDI GCAATG 1 cut(s) 42
BsrI ACTGG 1 cut(s) 439
BssECI CCNNGG 1 cut(s) 305
BssMI GATC 1 cut(s) 469
BssT1I CCWWGG 1 cut(s) 305
Bst2UI CCWGG 1 cut(s) 104
Bst4CI ACNGT 2 cut(s) 55, 285
BstBAI YACGTR 1 cut(s) 496
BstC8I GCNNGC 1 cut(s) 233
BstFNI CGCG 1 cut(s) 490
BstHHI GCGC 1 cut(s) 490
BstKTI GATC 1 cut(s) 472
BstMBI GATC 1 cut(s) 469
BstMWI GCNNNNNNNGC 2 cut(s) 32, 41
BstNI CCWGG 1 cut(s) 104
BstNSI RCATGY 2 cut(s) 184, 343
BstSCI CCNGG 2 cut(s) 102, 390
BstUI CGCG 1 cut(s) 490
BtsI GCAGTG 1 cut(s) 242
BtsIMutI CAGTG 2 cut(s) 242, 432
Cac8I GCNNGC 1 cut(s) 233
CfoI GCGC 1 cut(s) 490
CseI GACGC 1 cut(s) 264
CsiI ACCWGGT 1 cut(s) 102
Csp6I GTAC 1 cut(s) 82
CviAII CATG 4 cut(s) 181, 198, 340, 358
CviJI RGCY 7 cut(s) 93, 110, 189, 207, 231, 292, 366
CviKI_1 RGCY 7 cut(s) 93, 110, 189, 207, 231, 292, 366
CviQI GTAC 1 cut(s) 82
DpnI GATC 1 cut(s) 471
DpnII GATC 1 cut(s) 469
DraIII CACNNNGTG 1 cut(s) 73
DrdI GACNNNNNNGTC 1 cut(s) 58
DseDI GACNNNNNNGTC 1 cut(s) 58
Eco130I CCWWGG 1 cut(s) 305
EcoRII CCWGG 1 cut(s) 102
EcoT14I CCWWGG 1 cut(s) 305
ErhI CCWWGG 1 cut(s) 305
FaeI CATG 4 cut(s) 184, 201, 343, 361
FatI CATG 4 cut(s) 180, 197, 339, 357
FauI CCCGC 1 cut(s) 157
FauNDI CATATG 1 cut(s) 312
Fnu4HI GCNGC 1 cut(s) 354
Fsp4HI GCNGC 1 cut(s) 354
FspBI CTAG 1 cut(s) 173
GlaI GCGC 1 cut(s) 489
GluI GCNGC 1 cut(s) 354
HapII CCGG 3 cut(s) 120, 363, 391
HgaI GACGC 1 cut(s) 264
HhaI GCGC 1 cut(s) 490
Hin1II CATG 4 cut(s) 184, 201, 343, 361
Hin6I GCGC 1 cut(s) 488
HinP1I GCGC 1 cut(s) 488
HinfI GANTC 2 cut(s) 12, 438
HpaII CCGG 3 cut(s) 120, 363, 391
HphI GGTGA 3 cut(s) 41, 92, 202
Hpy166II GTNNAC 1 cut(s) 88
Hpy188I TCNGA 3 cut(s) 11, 131, 280
Hpy188III TCNNGA 2 cut(s) 120, 173
Hpy8I GTNNAC 1 cut(s) 88
HpyCH4III ACNGT 2 cut(s) 55, 285
HpyCH4IV ACGT 3 cut(s) 72, 84, 495
HpyCH4V TGCA 2 cut(s) 76, 235
HpyF10VI GCNNNNNNNGC 2 cut(s) 32, 41
HpySE526I ACGT 3 cut(s) 72, 84, 495
Hsp92II CATG 4 cut(s) 184, 201, 343, 361
HspAI GCGC 1 cut(s) 488
Kpn2I TCCGGA 1 cut(s) 119
Kzo9I GATC 1 cut(s) 469
LmnI GCTCC 3 cut(s) 297, 307, 336
LweI GCATC 1 cut(s) 468
MabI ACCWGGT 1 cut(s) 102
MaeI CTAG 1 cut(s) 173
MaeII ACGT 3 cut(s) 72, 84, 495
MaeIII GTNAC 1 cut(s) 491
MalI GATC 1 cut(s) 471
MboI GATC 1 cut(s) 469
MboII GAAGA 1 cut(s) 454
MhlI GDGCHC 1 cut(s) 333
MluCI AATT 3 cut(s) 216, 297, 413
MlyI GAGTC 1 cut(s) 432
MnlI CCTC 6 cut(s) 169, 200, 282, 398, 458, 461
MroI TCCGGA 1 cut(s) 119
MspI CCGG 3 cut(s) 120, 363, 391
MspR9I CCNGG 2 cut(s) 104, 392
MvaI CCWGG 1 cut(s) 104
MvnI CGCG 1 cut(s) 490
MwoI GCNNNNNNNGC 2 cut(s) 32, 41
NciI CCSGG 1 cut(s) 392
NdeI CATATG 1 cut(s) 312
NdeII GATC 1 cut(s) 469
NlaIII CATG 4 cut(s) 184, 201, 343, 361
NlaIV GGNNCC 2 cut(s) 152, 293
NspI RCATGY 2 cut(s) 184, 343
PciI ACATGT 2 cut(s) 180, 339
PfeI GAWTC 1 cut(s) 12
PflMI CCANNNNNTGG 1 cut(s) 160
PfoI TCCNGGA 1 cut(s) 390
PkrI GCNGC 1 cut(s) 355
PleI GAGTC 1 cut(s) 432
PpsI GAGTC 1 cut(s) 432
Ppu21I YACGTR 1 cut(s) 496
PscI ACATGT 2 cut(s) 180, 339
Psp6I CCWGG 1 cut(s) 102
PspGI CCWGG 1 cut(s) 102
PspN4I GGNNCC 2 cut(s) 152, 293
RsaI GTAC 1 cut(s) 83
RsaNI GTAC 1 cut(s) 82
SatI GCNGC 1 cut(s) 354
Sau3AI GATC 1 cut(s) 469
SchI GAGTC 1 cut(s) 432
ScrFI CCNGG 2 cut(s) 104, 392
SduI GDGCHC 1 cut(s) 333
SetI ASST 6 cut(s) 75, 87, 105, 112, 152, 498
SexAI ACCWGGT 1 cut(s) 102
SfaNI GCATC 1 cut(s) 468
Sse9I AATT 3 cut(s) 216, 297, 413
SsiI CCGC 2 cut(s) 164, 354
SspI AATATT 1 cut(s) 126
SspMI CTAG 1 cut(s) 173
StyD4I CCNGG 2 cut(s) 102, 390
StyI CCWWGG 1 cut(s) 305
TaaI ACNGT 2 cut(s) 55, 285
TaiI ACGT 3 cut(s) 75, 87, 498
TaqI TCGA 1 cut(s) 287
TasI AATT 3 cut(s) 216, 297, 413
TauI GCSGC 1 cut(s) 356
TfiI GAWTC 1 cut(s) 12
TscAI CASTG 2 cut(s) 242, 439
TspDTI ATGAA 2 cut(s) 4, 186
TspRI CASTG 2 cut(s) 242, 439
Van91I CCANNNNNTGG 1 cut(s) 160
XbaI TCTAGA 1 cut(s) 172
XceI RCATGY 2 cut(s) 184, 343
XcmI CCANNNNNNNNNTGG 1 cut(s) 200
XspI CTAG 1 cut(s) 173
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.