Rh4DG397200

transmembrane ascorbate ferrireductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0016051
Physical Location & Seq
Forward (+)
61377156 .. 61378778
1623 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG397200.1

Sequence Viewer

Length: 678 bp
ATGTATACTAATACAAACTATAGGTACCAGCGTTCAGCCTCTAGATTAACAATAGGTGCACACTTGTTTGGCATCTTAGCCCTCATACTCTTGCTTGTTTGGTTGTTGCATTATCGCGGGGGTCTCGATTATGATTCTGACAATGCAGAGCAAGTTTTCAATGTTCATCCATTTCTTATGTTCTTCGGATTCATTTTCTTTGCTGGTGAAGCAATGATGGCATACAAGACTGTAAAGTCGCAACACAACGTGCAGAAGTACGTTCACGGCTTCTTTCACCTGGTAGCTCTTTGTTCCGGAATATTCGGAATATGTGCTGTTTTCAGGTTCCACGATATGGCGGGAAATCTAGAGGACATGTATAGCCTCCATTCATGGATTGGCTTGACCACAATTATCTTGTTCGGCTTGCAGTGGTTGTTTGGAATTGCTACATTCTTGTTTCCAAGAGCGTCAGAACAGTCGAGGCTCCAAATTGCTCCTTGGCATATGAGTTTCGGCAGGGTGCTCCTATACATGTCAATATGTGCTGCCATGTCCGGCTTGATGGAGAAATCCACATTCCTCCGGGATAAGATAAGTGAAAGAGAATTACATCTCATCAACTTCACTGGACTCTCAATCCTCCTCTTTGGCATCTTCGTTGATCTTTCAGTTGCTCTGGCGCGTTACGTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0008150 GO:0008152 GO:0016491 GO:0055114
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cytochrom_B561

Protein Analysis

225

Amino Acids

25.78

Weight (kDa)

8.45

Isoelectric Point (pI)

38.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cytochrom_B561 PF03188 55 - 188 1.8e-40 Eukaryotic cytochrome b561
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0016051)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G14730
malus_domestica MD13G1016600.v1.1 MD16G1014500.v1.1
prunus_persica Prupe.1G336800_v2.0.a1
pyrus_communis pycom16g01330
rosa_chinensis RchiOBHm_Chr4g0442131
rosa_laevigata RLG00000006038
rosa_multiflora Rmu_sc0010284.1_g000013
rosa_roxburghii Rroxscaffold_5G00383040
rosa_rugosa Rorug04G0339200
rosa_samantha Rh4AG390600 Rh4BG404200 Rh4CG419200 Rh4DG397200
rosa_wichuraiana Rw4G033760

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 235
Acc65I GGTACC 1 cut(s) 24
AccB1I GGYRCC 1 cut(s) 24
AccB7I CCANNNNNTGG 1 cut(s) 337
AccI GTMKAC 1 cut(s) 5
AccII CGCG 2 cut(s) 117, 667
AccIII TCCGGA 1 cut(s) 296
AciI CCGC 2 cut(s) 117, 341
AdeI CACNNNGTG 1 cut(s) 250
AfaI GTAC 2 cut(s) 26, 260
AfiI CCNNNNNNNGG 1 cut(s) 337
AflIII ACRYGT 3 cut(s) 357, 516, 672
AgsI TTSAA 1 cut(s) 160
AjnI CCWGG 1 cut(s) 279
AluBI AGCT 1 cut(s) 287
AluI AGCT 1 cut(s) 287
Alw21I GWGCWC 2 cut(s) 61, 510
Alw26I GTCTC 1 cut(s) 128
Alw44I GTGCAC 1 cut(s) 57
Aor13HI TCCGGA 1 cut(s) 296
ApaLI GTGCAC 1 cut(s) 57
ApeKI GCWGC 1 cut(s) 530
Asp718I GGTACC 1 cut(s) 24
AspLEI GCGC 1 cut(s) 667
AsuC2I CCSGG 1 cut(s) 569
AsuHPI GGTGA 2 cut(s) 218, 269
BaeGI GKGCMC 1 cut(s) 61
BaeI ACNNNNGTAYC 2 cut(s) 16, 49
BanI GGYRCC 1 cut(s) 24
Bbv12I GWGCWC 2 cut(s) 61, 510
BbvI GCAGC 1 cut(s) 517
BccI CCATC 2 cut(s) 211, 541
BceAI ACGGC 1 cut(s) 283
BciT130I CCWGG 1 cut(s) 281
BcnI CCSGG 1 cut(s) 569
BcoDI GTCTC 1 cut(s) 128
BfaI CTAG 2 cut(s) 42, 350
BfmI CTRYAG 1 cut(s) 19
BisI GCNGC 1 cut(s) 531
BlsI GCNGC 1 cut(s) 532
Bme1390I CCNGG 2 cut(s) 281, 569
BmiI GGNNCC 3 cut(s) 26, 329, 470
BmrFI CCNGG 2 cut(s) 281, 569
BmsI GCATC 2 cut(s) 81, 645
BpuMI CCSGG 1 cut(s) 569
BsaAI YACGTR 1 cut(s) 673
BsaI GGTCTC 1 cut(s) 128
BsaJI CCNNGG 1 cut(s) 482
BsaWI WCCGGW 1 cut(s) 296
Bsc4I CCNNNNNNNGG 1 cut(s) 337
Bse1I ACTGG 1 cut(s) 616
Bse3DI GCAATG 1 cut(s) 219
BseAI TCCGGA 1 cut(s) 296
BseBI CCWGG 1 cut(s) 281
BseDI CCNNGG 1 cut(s) 482
BseGI GGATG 1 cut(s) 166
BseLI CCNNNNNNNGG 1 cut(s) 337
BseMI GCAATG 1 cut(s) 219
BseNI ACTGG 1 cut(s) 616
BseRI GAGGAG 1 cut(s) 617
BseSI GKGCMC 1 cut(s) 61
BseXI GCAGC 1 cut(s) 517
BsgI GTGCAG 1 cut(s) 272
Bsh1236I CGCG 2 cut(s) 117, 667
BshNI GGYRCC 1 cut(s) 24
BsiHKAI GWGCWC 2 cut(s) 61, 510
BsiSI CCGG 3 cut(s) 297, 540, 568
BslI CCNNNNNNNGG 1 cut(s) 337
BsmAI GTCTC 1 cut(s) 128
Bso31I GGTCTC 1 cut(s) 128
Bsp1286I GDGCHC 2 cut(s) 61, 510
Bsp13I TCCGGA 1 cut(s) 296
Bsp143I GATC 1 cut(s) 646
BspACI CCGC 2 cut(s) 117, 341
BspEI TCCGGA 1 cut(s) 296
BspFNI CGCG 2 cut(s) 117, 667
BspLI GGNNCC 3 cut(s) 26, 329, 470
BspT107I GGYRCC 1 cut(s) 24
BspTNI GGTCTC 1 cut(s) 128
BsrDI GCAATG 1 cut(s) 219
BsrI ACTGG 1 cut(s) 616
BssECI CCNNGG 1 cut(s) 482
BssMI GATC 1 cut(s) 646
BssNAI GTATAC 1 cut(s) 6
BssT1I CCWWGG 1 cut(s) 482
Bst1107I GTATAC 1 cut(s) 6
Bst2UI CCWGG 1 cut(s) 281
Bst4CI ACNGT 2 cut(s) 232, 462
BstBAI YACGTR 1 cut(s) 673
BstC8I GCNNGC 1 cut(s) 410
BstDEI CTNAG 1 cut(s) 76
BstF5I GGATG 1 cut(s) 166
BstFNI CGCG 2 cut(s) 117, 667
BstHHI GCGC 1 cut(s) 667
BstKTI GATC 1 cut(s) 649
BstMAI GTCTC 1 cut(s) 128
BstMBI GATC 1 cut(s) 646
BstMWI GCNNNNNNNGC 2 cut(s) 209, 218
BstNI CCWGG 1 cut(s) 281
BstNSI RCATGY 2 cut(s) 361, 520
BstSCI CCNGG 2 cut(s) 279, 567
BstSFI CTRYAG 1 cut(s) 19
BstSLI GKGCMC 1 cut(s) 61
BstUI CGCG 2 cut(s) 117, 667
BstV1I GCAGC 1 cut(s) 517
BstZ17I GTATAC 1 cut(s) 6
BtsCI GGATG 1 cut(s) 166
BtsI GCAGTG 1 cut(s) 419
BtsIMutI CAGTG 2 cut(s) 419, 609
Cac8I GCNNGC 1 cut(s) 410
CfoI GCGC 1 cut(s) 667
CseI GACGC 1 cut(s) 441
CsiI ACCWGGT 1 cut(s) 279
Csp6I GTAC 2 cut(s) 25, 259
CviAII CATG 4 cut(s) 358, 375, 517, 535
CviJI RGCY 9 cut(s) 38, 80, 270, 287, 366, 384, 408, 469, 543
CviKI_1 RGCY 9 cut(s) 38, 80, 270, 287, 366, 384, 408, 469, 543
CviQI GTAC 2 cut(s) 25, 259
DdeI CTNAG 1 cut(s) 76
DpnI GATC 1 cut(s) 648
DpnII GATC 1 cut(s) 646
DraIII CACNNNGTG 1 cut(s) 250
DrdI GACNNNNNNGTC 1 cut(s) 235
DseDI GACNNNNNNGTC 1 cut(s) 235
Eco130I CCWWGG 1 cut(s) 482
Eco31I GGTCTC 1 cut(s) 128
EcoRII CCWGG 1 cut(s) 279
EcoT14I CCWWGG 1 cut(s) 482
ErhI CCWWGG 1 cut(s) 482
FaeI CATG 4 cut(s) 361, 378, 520, 538
FatI CATG 4 cut(s) 357, 374, 516, 534
FauI CCCGC 2 cut(s) 110, 334
FauNDI CATATG 1 cut(s) 489
FblI GTMKAC 1 cut(s) 5
Fnu4HI GCNGC 1 cut(s) 531
FokI GGATG 1 cut(s) 153
Fsp4HI GCNGC 1 cut(s) 531
FspBI CTAG 2 cut(s) 42, 350
GlaI GCGC 1 cut(s) 666
GluI GCNGC 1 cut(s) 531
HapII CCGG 3 cut(s) 297, 540, 568
HgaI GACGC 1 cut(s) 441
HhaI GCGC 1 cut(s) 667
Hin1II CATG 4 cut(s) 361, 378, 520, 538
Hin6I GCGC 1 cut(s) 665
HinP1I GCGC 1 cut(s) 665
HinfI GANTC 3 cut(s) 134, 189, 615
HpaII CCGG 3 cut(s) 297, 540, 568
HphI GGTGA 2 cut(s) 218, 269
Hpy166II GTNNAC 3 cut(s) 6, 59, 265
Hpy188I TCNGA 4 cut(s) 139, 188, 308, 457
Hpy188III TCNNGA 4 cut(s) 42, 125, 297, 350
Hpy8I GTNNAC 3 cut(s) 6, 59, 265
HpyCH4III ACNGT 2 cut(s) 232, 462
HpyCH4IV ACGT 3 cut(s) 249, 261, 672
HpyCH4V TGCA 5 cut(s) 59, 109, 146, 253, 412
HpyF10VI GCNNNNNNNGC 2 cut(s) 209, 218
HpyF3I CTNAG 1 cut(s) 76
HpySE526I ACGT 3 cut(s) 249, 261, 672
Hsp92II CATG 4 cut(s) 361, 378, 520, 538
HspAI GCGC 1 cut(s) 665
Kpn2I TCCGGA 1 cut(s) 296
KpnI GGTACC 1 cut(s) 28
Kzo9I GATC 1 cut(s) 646
LmnI GCTCC 3 cut(s) 474, 484, 513
Lsp1109I GCAGC 1 cut(s) 517
LweI GCATC 2 cut(s) 81, 645
MabI ACCWGGT 1 cut(s) 279
MaeI CTAG 2 cut(s) 42, 350
MaeII ACGT 3 cut(s) 249, 261, 672
MaeIII GTNAC 1 cut(s) 668
MalI GATC 1 cut(s) 648
MboI GATC 1 cut(s) 646
MboII GAAGA 2 cut(s) 175, 631
MhlI GDGCHC 2 cut(s) 61, 510
MluCI AATT 4 cut(s) 393, 426, 474, 590
MlyI GAGTC 1 cut(s) 609
MnlI CCTC 8 cut(s) 49, 92, 346, 377, 459, 575, 635, 638
MroI TCCGGA 1 cut(s) 296
MseI TTAA 1 cut(s) 47
MspI CCGG 3 cut(s) 297, 540, 568
MspR9I CCNGG 2 cut(s) 281, 569
MvaI CCWGG 1 cut(s) 281
MvnI CGCG 2 cut(s) 117, 667
MwoI GCNNNNNNNGC 2 cut(s) 209, 218
NciI CCSGG 1 cut(s) 569
NdeI CATATG 1 cut(s) 489
NdeII GATC 1 cut(s) 646
NlaIII CATG 4 cut(s) 361, 378, 520, 538
NlaIV GGNNCC 3 cut(s) 26, 329, 470
NspI RCATGY 2 cut(s) 361, 520
PciI ACATGT 2 cut(s) 357, 516
PfeI GAWTC 2 cut(s) 134, 189
PflMI CCANNNNNTGG 1 cut(s) 337
PfoI TCCNGGA 1 cut(s) 567
PkrI GCNGC 1 cut(s) 532
PleI GAGTC 1 cut(s) 609
PpsI GAGTC 1 cut(s) 609
Ppu21I YACGTR 1 cut(s) 673
PscI ACATGT 2 cut(s) 357, 516
Psp6I CCWGG 1 cut(s) 279
PspGI CCWGG 1 cut(s) 279
PspN4I GGNNCC 3 cut(s) 26, 329, 470
RsaI GTAC 2 cut(s) 26, 260
RsaNI GTAC 2 cut(s) 25, 259
SaqAI TTAA 1 cut(s) 47
SatI GCNGC 1 cut(s) 531
Sau3AI GATC 1 cut(s) 646
SchI GAGTC 1 cut(s) 609
ScrFI CCNGG 2 cut(s) 281, 569
SduI GDGCHC 2 cut(s) 61, 510
SetI ASST 8 cut(s) 26, 58, 252, 264, 282, 289, 329, 675
SexAI ACCWGGT 1 cut(s) 279
SfaNI GCATC 2 cut(s) 81, 645
SfcI CTRYAG 1 cut(s) 19
Sse9I AATT 4 cut(s) 393, 426, 474, 590
SsiI CCGC 2 cut(s) 117, 341
SspI AATATT 1 cut(s) 303
SspMI CTAG 2 cut(s) 42, 350
StyD4I CCNGG 2 cut(s) 279, 567
StyI CCWWGG 1 cut(s) 482
TaaI ACNGT 2 cut(s) 232, 462
TaiI ACGT 3 cut(s) 252, 264, 675
TaqI TCGA 2 cut(s) 126, 464
TasI AATT 4 cut(s) 393, 426, 474, 590
TfiI GAWTC 2 cut(s) 134, 189
Tru1I TTAA 1 cut(s) 47
Tru9I TTAA 1 cut(s) 47
TscAI CASTG 2 cut(s) 419, 616
TseI GCWGC 1 cut(s) 530
TspDTI ATGAA 3 cut(s) 155, 181, 363
TspRI CASTG 2 cut(s) 419, 616
Van91I CCANNNNNTGG 1 cut(s) 337
VneI GTGCAC 1 cut(s) 57
XbaI TCTAGA 2 cut(s) 41, 349
XceI RCATGY 2 cut(s) 361, 520
XcmI CCANNNNNNNNNTGG 1 cut(s) 377
XmiI GTMKAC 1 cut(s) 5
XspI CTAG 2 cut(s) 42, 350
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.