Rroxscaffold_5G00383040

transmembrane ascorbate ferrireductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0016051
Physical Location & Seq
Forward (+)
62749642 .. 62750888
1247 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00383040.1

Sequence Viewer

Length: 609 bp
ATGTATACTAATACAAACTATAGGTACCAGCGTTCAGCCTCTAGATTAACAATAGGTGCACACTTGTTTGGCATCTTAGCCCTCATTCTCTTGCTTGTTTGGTTGTTGCATTATCGCGGGGGTCTCGATTATGATTCTGACAATGCAGAGCAAGTTTTCAATGTTCATCCATTTCTTATGTTCTTCGGATTCATTTTCTTTGCTGGTGAAGCAATGATGGCATACAAGACTGTAAAGTCGCAACACAACGTGCAGAAGTTCCACGATATGGCCGGAAATCTAGAGGACATGTATAGCCTCCATTCATGGATTGGCTTGACCACAATTATCTTGTTCGGCTTGCAGTGGTTGTTTGGATTTGCTACATTCTTGTTTCCAAGAGCGTCAGAACAGTCGAGGCTCCAAATTGCTCCTTGGCATATGAGTTTCGGCAGGGTGCTCCTATACATGTCAATATGTGCCGCCATGTCCGGCTTGATGGAGAAATCCACATTCCTCCGGGATAAGATAAGTGAAAGAGAATTACATCTCATCAACTTCACTGGACTCTCAATCCTCCTCTTTGGCATCTTCGTTGATCTTTCAGTTGCTTTGGCGCGTTACGTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0008150 GO:0008152 GO:0016491 GO:0055114
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cytochrom_B561

Protein Analysis

202

Amino Acids

23.28

Weight (kDa)

7.93

Isoelectric Point (pI)

43.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cytochrom_B561 PF03188 55 - 85 2.2e-06 Eukaryotic cytochrome b561
Cytochrom_B561 PF03188 86 - 165 2.8e-22 Eukaryotic cytochrome b561
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016051)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G14730
malus_domestica MD13G1016600.v1.1 MD16G1014500.v1.1
prunus_persica Prupe.1G336800_v2.0.a1
pyrus_communis pycom16g01330
rosa_chinensis RchiOBHm_Chr4g0442131
rosa_laevigata RLG00000006038
rosa_multiflora Rmu_sc0010284.1_g000013
rosa_roxburghii Rroxscaffold_5G00383040
rosa_rugosa Rorug04G0339200
rosa_samantha Rh4AG390600 Rh4BG404200 Rh4CG419200 Rh4DG397200
rosa_wichuraiana Rw4G033760

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 235
Acc65I GGTACC 1 cut(s) 24
AccB1I GGYRCC 1 cut(s) 24
AccB7I CCANNNNNTGG 1 cut(s) 268
AccI GTMKAC 1 cut(s) 5
AccII CGCG 2 cut(s) 117, 598
AciI CCGC 2 cut(s) 117, 462
AcoI YGGCCR 1 cut(s) 270
AdeI CACNNNGTG 1 cut(s) 250
AfaI GTAC 1 cut(s) 26
AfiI CCNNNNNNNGG 1 cut(s) 268
AflIII ACRYGT 3 cut(s) 288, 447, 603
AgsI TTSAA 1 cut(s) 160
Alw21I GWGCWC 2 cut(s) 61, 441
Alw26I GTCTC 1 cut(s) 128
Alw44I GTGCAC 1 cut(s) 57
AoxI GGCC 1 cut(s) 270
ApaLI GTGCAC 1 cut(s) 57
Asp718I GGTACC 1 cut(s) 24
AspLEI GCGC 1 cut(s) 598
AsuC2I CCSGG 1 cut(s) 500
AsuHPI GGTGA 1 cut(s) 218
BaeGI GKGCMC 1 cut(s) 61
BaeI ACNNNNGTAYC 2 cut(s) 16, 49
BanI GGYRCC 1 cut(s) 24
Bbv12I GWGCWC 2 cut(s) 61, 441
BccI CCATC 2 cut(s) 211, 472
BcnI CCSGG 1 cut(s) 500
BcoDI GTCTC 1 cut(s) 128
BfaI CTAG 2 cut(s) 42, 281
BfmI CTRYAG 1 cut(s) 19
BisI GCNGC 1 cut(s) 462
BlsI GCNGC 1 cut(s) 463
Bme1390I CCNGG 1 cut(s) 500
BmiI GGNNCC 2 cut(s) 26, 401
BmrFI CCNGG 1 cut(s) 500
BmsI GCATC 2 cut(s) 81, 576
BpuMI CCSGG 1 cut(s) 500
BsaAI YACGTR 1 cut(s) 604
BsaI GGTCTC 1 cut(s) 128
BsaJI CCNNGG 1 cut(s) 413
Bsc4I CCNNNNNNNGG 1 cut(s) 268
Bse1I ACTGG 1 cut(s) 547
Bse3DI GCAATG 1 cut(s) 219
BseDI CCNNGG 1 cut(s) 413
BseGI GGATG 1 cut(s) 166
BseLI CCNNNNNNNGG 1 cut(s) 268
BseMI GCAATG 1 cut(s) 219
BseNI ACTGG 1 cut(s) 547
BseRI GAGGAG 1 cut(s) 548
BseSI GKGCMC 1 cut(s) 61
BsgI GTGCAG 1 cut(s) 272
Bsh1236I CGCG 2 cut(s) 117, 598
BshFI GGCC 1 cut(s) 272
BshNI GGYRCC 1 cut(s) 24
BsiHKAI GWGCWC 2 cut(s) 61, 441
BsiSI CCGG 3 cut(s) 273, 471, 499
BslI CCNNNNNNNGG 1 cut(s) 268
BsmAI GTCTC 1 cut(s) 128
BsnI GGCC 1 cut(s) 272
Bso31I GGTCTC 1 cut(s) 128
Bsp1286I GDGCHC 2 cut(s) 61, 441
Bsp143I GATC 1 cut(s) 577
BspACI CCGC 2 cut(s) 117, 462
BspANI GGCC 1 cut(s) 272
BspFNI CGCG 2 cut(s) 117, 598
BspLI GGNNCC 2 cut(s) 26, 401
BspT107I GGYRCC 1 cut(s) 24
BspTNI GGTCTC 1 cut(s) 128
BsrDI GCAATG 1 cut(s) 219
BsrI ACTGG 1 cut(s) 547
BssECI CCNNGG 1 cut(s) 413
BssMI GATC 1 cut(s) 577
BssNAI GTATAC 1 cut(s) 6
BssT1I CCWWGG 1 cut(s) 413
Bst1107I GTATAC 1 cut(s) 6
Bst4CI ACNGT 2 cut(s) 232, 393
BstBAI YACGTR 1 cut(s) 604
BstC8I GCNNGC 1 cut(s) 341
BstDEI CTNAG 1 cut(s) 76
BstF5I GGATG 1 cut(s) 166
BstFNI CGCG 2 cut(s) 117, 598
BstHHI GCGC 1 cut(s) 598
BstKTI GATC 1 cut(s) 580
BstMAI GTCTC 1 cut(s) 128
BstMBI GATC 1 cut(s) 577
BstMWI GCNNNNNNNGC 2 cut(s) 209, 218
BstNSI RCATGY 2 cut(s) 292, 451
BstSCI CCNGG 1 cut(s) 498
BstSFI CTRYAG 1 cut(s) 19
BstSLI GKGCMC 1 cut(s) 61
BstUI CGCG 2 cut(s) 117, 598
BstZ17I GTATAC 1 cut(s) 6
BsuRI GGCC 1 cut(s) 272
BtsCI GGATG 1 cut(s) 166
BtsI GCAGTG 1 cut(s) 350
BtsIMutI CAGTG 2 cut(s) 350, 540
Cac8I GCNNGC 1 cut(s) 341
CfoI GCGC 1 cut(s) 598
CseI GACGC 1 cut(s) 372
Csp6I GTAC 1 cut(s) 25
CviAII CATG 4 cut(s) 289, 306, 448, 466
CviJI RGCY 8 cut(s) 38, 80, 272, 297, 315, 339, 400, 474
CviKI_1 RGCY 8 cut(s) 38, 80, 272, 297, 315, 339, 400, 474
CviQI GTAC 1 cut(s) 25
DdeI CTNAG 1 cut(s) 76
DpnI GATC 1 cut(s) 579
DpnII GATC 1 cut(s) 577
DraIII CACNNNGTG 1 cut(s) 250
DrdI GACNNNNNNGTC 1 cut(s) 235
DseDI GACNNNNNNGTC 1 cut(s) 235
EaeI YGGCCR 1 cut(s) 270
Eco130I CCWWGG 1 cut(s) 413
Eco31I GGTCTC 1 cut(s) 128
EcoT14I CCWWGG 1 cut(s) 413
ErhI CCWWGG 1 cut(s) 413
FaeI CATG 4 cut(s) 292, 309, 451, 469
FatI CATG 4 cut(s) 288, 305, 447, 465
FauI CCCGC 1 cut(s) 110
FauNDI CATATG 1 cut(s) 420
FblI GTMKAC 1 cut(s) 5
Fnu4HI GCNGC 1 cut(s) 462
FokI GGATG 1 cut(s) 153
Fsp4HI GCNGC 1 cut(s) 462
FspBI CTAG 2 cut(s) 42, 281
GlaI GCGC 1 cut(s) 597
GluI GCNGC 1 cut(s) 462
HaeIII GGCC 1 cut(s) 272
HapII CCGG 3 cut(s) 273, 471, 499
HgaI GACGC 1 cut(s) 372
HhaI GCGC 1 cut(s) 598
Hin1II CATG 4 cut(s) 292, 309, 451, 469
Hin6I GCGC 1 cut(s) 596
HinP1I GCGC 1 cut(s) 596
HinfI GANTC 3 cut(s) 134, 189, 546
HpaII CCGG 3 cut(s) 273, 471, 499
HphI GGTGA 1 cut(s) 218
Hpy166II GTNNAC 2 cut(s) 6, 59
Hpy188I TCNGA 3 cut(s) 139, 188, 388
Hpy188III TCNNGA 3 cut(s) 42, 125, 281
Hpy8I GTNNAC 2 cut(s) 6, 59
HpyCH4III ACNGT 2 cut(s) 232, 393
HpyCH4IV ACGT 2 cut(s) 249, 603
HpyCH4V TGCA 5 cut(s) 59, 109, 146, 253, 343
HpyF10VI GCNNNNNNNGC 2 cut(s) 209, 218
HpyF3I CTNAG 1 cut(s) 76
HpySE526I ACGT 2 cut(s) 249, 603
Hsp92II CATG 4 cut(s) 292, 309, 451, 469
HspAI GCGC 1 cut(s) 596
KpnI GGTACC 1 cut(s) 28
Kzo9I GATC 1 cut(s) 577
LmnI GCTCC 3 cut(s) 405, 415, 444
LpnPI CCDG 7 cut(s) 41, 189, 286, 418, 484, 512, 528
LweI GCATC 2 cut(s) 81, 576
MaeI CTAG 2 cut(s) 42, 281
MaeII ACGT 2 cut(s) 249, 603
MaeIII GTNAC 1 cut(s) 599
MalI GATC 1 cut(s) 579
MboI GATC 1 cut(s) 577
MboII GAAGA 2 cut(s) 175, 562
MhlI GDGCHC 2 cut(s) 61, 441
MluCI AATT 3 cut(s) 324, 405, 521
MlyI GAGTC 1 cut(s) 540
MnlI CCTC 8 cut(s) 49, 92, 277, 308, 390, 506, 566, 569
MseI TTAA 1 cut(s) 47
MspI CCGG 3 cut(s) 273, 471, 499
MspR9I CCNGG 1 cut(s) 500
MvnI CGCG 2 cut(s) 117, 598
MwoI GCNNNNNNNGC 2 cut(s) 209, 218
NciI CCSGG 1 cut(s) 500
NdeI CATATG 1 cut(s) 420
NdeII GATC 1 cut(s) 577
NlaIII CATG 4 cut(s) 292, 309, 451, 469
NlaIV GGNNCC 2 cut(s) 26, 401
NspI RCATGY 2 cut(s) 292, 451
PciI ACATGT 2 cut(s) 288, 447
PfeI GAWTC 2 cut(s) 134, 189
PflMI CCANNNNNTGG 1 cut(s) 268
PfoI TCCNGGA 1 cut(s) 498
PkrI GCNGC 1 cut(s) 463
PleI GAGTC 1 cut(s) 540
PpsI GAGTC 1 cut(s) 540
Ppu21I YACGTR 1 cut(s) 604
PscI ACATGT 2 cut(s) 288, 447
PspN4I GGNNCC 2 cut(s) 26, 401
RsaI GTAC 1 cut(s) 26
RsaNI GTAC 1 cut(s) 25
SaqAI TTAA 1 cut(s) 47
SatI GCNGC 1 cut(s) 462
Sau3AI GATC 1 cut(s) 577
SchI GAGTC 1 cut(s) 540
ScrFI CCNGG 1 cut(s) 500
SduI GDGCHC 2 cut(s) 61, 441
SetI ASST 4 cut(s) 26, 58, 252, 606
SfaNI GCATC 2 cut(s) 81, 576
SfcI CTRYAG 1 cut(s) 19
Sse9I AATT 3 cut(s) 324, 405, 521
SsiI CCGC 2 cut(s) 117, 462
SspMI CTAG 2 cut(s) 42, 281
StyD4I CCNGG 1 cut(s) 498
StyI CCWWGG 1 cut(s) 413
TaaI ACNGT 2 cut(s) 232, 393
TaiI ACGT 2 cut(s) 252, 606
TaqI TCGA 2 cut(s) 126, 395
TasI AATT 3 cut(s) 324, 405, 521
TauI GCSGC 1 cut(s) 464
TfiI GAWTC 2 cut(s) 134, 189
Tru1I TTAA 1 cut(s) 47
Tru9I TTAA 1 cut(s) 47
TscAI CASTG 2 cut(s) 350, 547
TspDTI ATGAA 3 cut(s) 155, 181, 294
TspRI CASTG 2 cut(s) 350, 547
Van91I CCANNNNNTGG 1 cut(s) 268
VneI GTGCAC 1 cut(s) 57
XbaI TCTAGA 2 cut(s) 41, 280
XceI RCATGY 2 cut(s) 292, 451
XcmI CCANNNNNNNNNTGG 1 cut(s) 308
XmiI GTMKAC 1 cut(s) 5
XspI CTAG 2 cut(s) 42, 281
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.