MD13G1019700.v1.1

CRAL/TRIO domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0013330
Physical Location & Seq
Reverse (-)
1241673 .. 1245201
3529 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1019700.v1.1.491

Sequence Viewer

Length: 732 bp
ATGGAGAAAACCCAAGAAATTGCTCTGACCGAGATGAAGAAATCAGTGCAAAAGCTTGGATCTTCCACTGAGAAGTTTGGAGACCCAACACTCATGAGGTTCTTGATTGCGAGATCAATGGACCCGGACAAAGCAGCGAAGATGTTTGTTCAGTGGCACAAATGGAGGGCTTCTTTTGTTCCAAATGGGTTCGTTTTGGACGCCGAAGTGAAGGATGAATTGGACGACAGAAAGATCTTCTTGCAGGGTTTGTCCAAGGACGGGTACCCTGTCATGGTGGTGAAAGCAAGCAAGCATTTTCCTTCCAAGGACCAGCTCCAATTCAAGAAATTTGTGGTTCATCTGCTTGACAAAACAATTGCAAGCTCTTTCAGGGGAAAGGAAATTGGAAATGAAAAGATGATCGGCATTCTTGATTTGCAACAAATGTCATACAAAAATATTGATGCACGTGGGTTAATCACTGGATTTCAATTTCTACAGTCTTACTACCCCGAGCGATTAGCAAAGTGCTTCATCTTAAACATGCCACGGTTTTTTGTAAGTGTCTGGAGGATGGTTTCTCGCTTCCTCGAGAAGGCAACACAAGAAAAGGTTGTGATTGTGACCAGTGAGGAAGAAACAAAAAACTTCATCAGGGAAATCGGTGAAGAAACCTTGCCAGAAGAGTATGGAGGACGAGCAAAGCTTGTACCTCTGCAAGATGTTATACTGGCACCAGTGGAGGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CRAL_TRIO

Protein Analysis

244

Amino Acids

28.01

Weight (kDa)

8.76

Isoelectric Point (pI)

48.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CRAL_TRIO PF00650 76 - 226 1e-27 CRAL/TRIO domain
CRAL_TRIO_2 PF13716 88 - 226 1.1e-09 Divergent CRAL/TRIO domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013330)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G14820 AT1G14820 AT1G14820
fragaria_vesca FvH4_4g33460 FvH4_4g33460
malus_domestica MD13G1019700.v1.1 MD16G1017800.v1.1
prunus_persica Prupe.1G334300_v2.0.a1
pyrus_communis pycom13g01670
rosa_chinensis RchiOBHm_Chr4g0442771
rosa_laevigata RLG00000005985
rosa_roxburghii Rroxscaffold_5G00383550
rosa_rugosa Rorug04G0342900
rosa_samantha Rh4AG395500 Rh4BG408200 Rh4CG424000 Rh4DG402400
rosa_wichuraiana Rw4G034180

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 264
AccB1I GGYRCC 2 cut(s) 264, 715
AclWI GGATC 1 cut(s) 67
AcsI RAATTY 1 cut(s) 329
AcvI CACGTG 1 cut(s) 452
AcyI GRCGYC 1 cut(s) 201
AfaI GTAC 2 cut(s) 266, 693
AfiI CCNNNNNNNGG 3 cut(s) 261, 274, 577
AgsI TTSAA 2 cut(s) 325, 473
AjuI GAANNNNNNNTTGG 2 cut(s) 203, 235
AluBI AGCT 4 cut(s) 55, 316, 366, 688
AluI AGCT 4 cut(s) 55, 316, 366, 688
Alw26I GTCTC 1 cut(s) 75
AlwI GGATC 1 cut(s) 67
Ama87I CYCGRG 2 cut(s) 494, 572
ApeKI GCWGC 1 cut(s) 134
ApoI RAATTY 1 cut(s) 329
Asp718I GGTACC 1 cut(s) 264
AspS9I GGNCC 2 cut(s) 121, 310
AsuC2I CCSGG 1 cut(s) 125
AsuHPI GGTGA 2 cut(s) 292, 659
AvaI CYCGRG 2 cut(s) 494, 572
AvaII GGWCC 2 cut(s) 121, 310
BanI GGYRCC 2 cut(s) 264, 715
BbrPI CACGTG 1 cut(s) 452
BbvI GCAGC 1 cut(s) 146
BccI CCATC 1 cut(s) 550
BcnI CCSGG 1 cut(s) 125
BcoDI GTCTC 1 cut(s) 75
BfmI CTRYAG 1 cut(s) 479
BglII AGATCT 1 cut(s) 234
BisI GCNGC 1 cut(s) 135
BlsI GCNGC 1 cut(s) 136
Bme1390I CCNGG 1 cut(s) 125
Bme18I GGWCC 2 cut(s) 121, 310
BmeT110I CYCGRG 2 cut(s) 494, 572
BmgT120I GGNCC 2 cut(s) 121, 310
BmiI GGNNCC 3 cut(s) 123, 266, 717
BmrFI CCNGG 1 cut(s) 125
BmsI GCATC 1 cut(s) 436
BpmI CTGGAG 1 cut(s) 571
BpuMI CCSGG 1 cut(s) 125
BsaAI YACGTR 1 cut(s) 452
BsaHI GRCGYC 1 cut(s) 201
BsaI GGTCTC 1 cut(s) 75
BsaJI CCNNGG 3 cut(s) 255, 306, 530
Bsc4I CCNNNNNNNGG 3 cut(s) 261, 274, 577
Bse1I ACTGG 4 cut(s) 469, 609, 717, 719
BseDI CCNNGG 3 cut(s) 255, 306, 530
BseGI GGATG 2 cut(s) 220, 561
BseLI CCNNNNNNNGG 3 cut(s) 261, 274, 577
BseMII CTCAG 1 cut(s) 60
BseNI ACTGG 4 cut(s) 469, 609, 717, 719
BseXI GCAGC 1 cut(s) 146
BshNI GGYRCC 2 cut(s) 264, 715
BsiHKCI CYCGRG 2 cut(s) 494, 572
BsiSI CCGG 1 cut(s) 125
BslI CCNNNNNNNGG 3 cut(s) 261, 274, 577
BsmAI GTCTC 1 cut(s) 75
BsmI GAATGC 1 cut(s) 408
Bso31I GGTCTC 1 cut(s) 75
BsoBI CYCGRG 2 cut(s) 494, 572
Bsp143I GATC 4 cut(s) 59, 113, 234, 402
BspCNI CTCAG 1 cut(s) 61
BspHI TCATGA 1 cut(s) 93
BspLI GGNNCC 3 cut(s) 123, 266, 717
BspPI GGATC 1 cut(s) 67
BspT107I GGYRCC 2 cut(s) 264, 715
BspTNI GGTCTC 1 cut(s) 75
BsrI ACTGG 4 cut(s) 469, 609, 717, 719
BssECI CCNNGG 3 cut(s) 255, 306, 530
BssMI GATC 4 cut(s) 59, 113, 234, 402
BssNI GRCGYC 1 cut(s) 201
BssT1I CCWWGG 2 cut(s) 255, 306
Bst4CI ACNGT 2 cut(s) 483, 534
Bst6I CTCTTC 1 cut(s) 660
BstACI GRCGYC 1 cut(s) 201
BstBAI YACGTR 1 cut(s) 452
BstC8I GCNNGC 3 cut(s) 289, 293, 364
BstDEI CTNAG 1 cut(s) 69
BstDSI CCRYGG 1 cut(s) 530
BstENI CCTNNNNNAGG 1 cut(s) 575
BstF5I GGATG 2 cut(s) 220, 561
BstKTI GATC 4 cut(s) 62, 116, 237, 405
BstMAI GTCTC 1 cut(s) 75
BstMBI GATC 4 cut(s) 59, 113, 234, 402
BstNSI RCATGY 1 cut(s) 529
BstSCI CCNGG 1 cut(s) 123
BstSFI CTRYAG 1 cut(s) 479
BstV1I GCAGC 1 cut(s) 146
BstX2I RGATCY 2 cut(s) 59, 234
BstYI RGATCY 2 cut(s) 59, 234
BtgI CCRYGG 1 cut(s) 530
BtsCI GGATG 2 cut(s) 220, 561
BtsIMutI CAGTG 6 cut(s) 51, 66, 158, 462, 616, 726
Cac8I GCNNGC 3 cut(s) 289, 293, 364
CciI TCATGA 1 cut(s) 93
Cfr13I GGNCC 2 cut(s) 121, 310
CseI GACGC 1 cut(s) 209
Csp6I GTAC 2 cut(s) 265, 692
CviAII CATG 3 cut(s) 94, 274, 526
CviJI RGCY 5 cut(s) 55, 170, 316, 366, 688
CviKI_1 RGCY 5 cut(s) 55, 170, 316, 366, 688
CviQI GTAC 2 cut(s) 265, 692
DdeI CTNAG 1 cut(s) 69
DpnI GATC 4 cut(s) 61, 115, 236, 404
DpnII GATC 4 cut(s) 59, 113, 234, 402
Eam1104I CTCTTC 1 cut(s) 660
EarI CTCTTC 1 cut(s) 660
Eco130I CCWWGG 2 cut(s) 255, 306
Eco31I GGTCTC 1 cut(s) 75
Eco47I GGWCC 2 cut(s) 121, 310
Eco72I CACGTG 1 cut(s) 452
Eco88I CYCGRG 2 cut(s) 494, 572
EcoNI CCTNNNNNAGG 1 cut(s) 575
EcoT14I CCWWGG 2 cut(s) 255, 306
ErhI CCWWGG 2 cut(s) 255, 306
FaeI CATG 3 cut(s) 97, 277, 529
FaiI YATR 6 cut(s) 95, 275, 433, 527, 672, 710
FalI AAGNNNNNCTT 2 cut(s) 224, 256
FatI CATG 3 cut(s) 93, 273, 525
Fnu4HI GCNGC 1 cut(s) 135
FokI GGATG 2 cut(s) 227, 568
Fsp4HI GCNGC 1 cut(s) 135
GluI GCNGC 1 cut(s) 135
GsuI CTGGAG 1 cut(s) 571
HapII CCGG 1 cut(s) 125
HgaI GACGC 1 cut(s) 209
Hin1I GRCGYC 1 cut(s) 201
Hin1II CATG 3 cut(s) 97, 277, 529
HindIII AAGCTT 2 cut(s) 53, 686
HpaII CCGG 1 cut(s) 125
HphI GGTGA 2 cut(s) 292, 659
Hpy188I TCNGA 1 cut(s) 27
Hpy188III TCNNGA 6 cut(s) 94, 103, 325, 413, 550, 574
HpyAV CCTTC 3 cut(s) 205, 312, 571
HpyCH4III ACNGT 2 cut(s) 483, 534
HpyCH4IV ACGT 1 cut(s) 451
HpyCH4V TGCA 6 cut(s) 49, 244, 362, 421, 449, 700
HpyF3I CTNAG 1 cut(s) 69
HpySE526I ACGT 1 cut(s) 451
Hsp92I GRCGYC 1 cut(s) 201
Hsp92II CATG 3 cut(s) 97, 277, 529
KpnI GGTACC 1 cut(s) 268
Kzo9I GATC 4 cut(s) 59, 113, 234, 402
LmnI GCTCC 1 cut(s) 321
Lsp1109I GCAGC 1 cut(s) 146
LweI GCATC 1 cut(s) 436
MaeII ACGT 1 cut(s) 451
MaeIII GTNAC 1 cut(s) 604
MalI GATC 4 cut(s) 61, 115, 236, 404
MboI GATC 4 cut(s) 59, 113, 234, 402
MboII GAAGA 7 cut(s) 49, 54, 151, 229, 629, 662, 677
MfeI CAATTG 1 cut(s) 357
MflI RGATCY 2 cut(s) 59, 234
MluCI AATT 7 cut(s) 18, 218, 320, 329, 357, 384, 473
MnlI CCTC 8 cut(s) 90, 159, 546, 581, 607, 668, 705, 718
MseI TTAA 2 cut(s) 458, 521
MslI CAYNNNNRTG 1 cut(s) 278
MspI CCGG 1 cut(s) 125
MspR9I CCNGG 1 cut(s) 125
MunI CAATTG 1 cut(s) 357
Mva1269I GAATGC 1 cut(s) 408
NciI CCSGG 1 cut(s) 125
NdeII GATC 4 cut(s) 59, 113, 234, 402
NlaIII CATG 3 cut(s) 97, 277, 529
NlaIV GGNNCC 3 cut(s) 123, 266, 717
NmuCI GTSAC 1 cut(s) 604
NspI RCATGY 1 cut(s) 529
PaeR7I CTCGAG 1 cut(s) 572
PagI TCATGA 1 cut(s) 93
PctI GAATGC 1 cut(s) 408
PkrI GCNGC 1 cut(s) 136
PmaCI CACGTG 1 cut(s) 452
PmlI CACGTG 1 cut(s) 452
Ppu21I YACGTR 1 cut(s) 452
PspCI CACGTG 1 cut(s) 452
PspN4I GGNNCC 3 cut(s) 123, 266, 717
PspPI GGNCC 2 cut(s) 121, 310
PsuI RGATCY 2 cut(s) 59, 234
RsaI GTAC 2 cut(s) 266, 693
RsaNI GTAC 2 cut(s) 265, 692
RseI CAYNNNNRTG 1 cut(s) 278
SaqAI TTAA 2 cut(s) 458, 521
SatI GCNGC 1 cut(s) 135
Sau3AI GATC 4 cut(s) 59, 113, 234, 402
Sau96I GGNCC 2 cut(s) 121, 310
ScrFI CCNGG 1 cut(s) 125
SetI ASST 9 cut(s) 57, 101, 318, 368, 454, 597, 659, 690, 697
SfaNI GCATC 1 cut(s) 436
SfcI CTRYAG 1 cut(s) 479
Sfr274I CTCGAG 1 cut(s) 572
SinI GGWCC 2 cut(s) 121, 310
SlaI CTCGAG 1 cut(s) 572
SmiMI CAYNNNNRTG 1 cut(s) 278
SmlI CTYRAG 1 cut(s) 572
SmoI CTYRAG 1 cut(s) 572
Sse9I AATT 7 cut(s) 18, 218, 320, 329, 357, 384, 473
SspI AATATT 1 cut(s) 442
StyD4I CCNGG 1 cut(s) 123
StyI CCWWGG 2 cut(s) 255, 306
TaaI ACNGT 2 cut(s) 483, 534
TaiI ACGT 1 cut(s) 454
TaqI TCGA 1 cut(s) 573
TaqII GACCGA 1 cut(s) 44
TasI AATT 7 cut(s) 18, 218, 320, 329, 357, 384, 473
Tru1I TTAA 2 cut(s) 458, 521
Tru9I TTAA 2 cut(s) 458, 521
TscAI CASTG 6 cut(s) 51, 73, 158, 469, 616, 726
TseFI GTSAC 1 cut(s) 604
TseI GCWGC 1 cut(s) 134
Tsp45I GTSAC 1 cut(s) 604
TspDTI ATGAA 6 cut(s) 50, 231, 329, 408, 505, 622
TspRI CASTG 6 cut(s) 51, 73, 158, 469, 616, 726
VpaK11BI GGWCC 2 cut(s) 121, 310
XagI CCTNNNNNAGG 1 cut(s) 575
XapI RAATTY 1 cut(s) 329
XceI RCATGY 1 cut(s) 529
XhoI CTCGAG 1 cut(s) 572
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.