Prupe.1G334300_v2.0.a1

CRAL/TRIO domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0013330
Physical Location & Seq
Forward (+)
31608164 .. 31610787
2624 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G334300.1

Sequence Viewer

Length: 732 bp
ATGGAGAAAAACCAGGAAATTGCACTGACTGAGATGAGGAAATCAGTTCAAAAGCTCGGGACTTCCACAGAGAAGTATGGAGACCCGACACTCATGAGGTTCTTGATTGCAAGATCAATGGACCCGGACAAAGCAGCAAAAATGTTTGTTCAGTGGCATAAATGGAGGGCCTCGTTTGTTCCAAATGGGTTCATTTCTGATTCCGAAGTAAGTGATGCATTGGAAGACAGAAAGATTTTTTTGCAGGGTTTGTCTAAGGATGGATACCCATTGATGATTGTGAAAGCAAGCAAGCATTTTCCTTCTAAGGATCATCTCCAATTCAAGAAATTTGTGGTTCATCTGCTTGACAAAACAATTGCAAGCTCTTTCAGAGGACGAGAAATTGGAAATGAAAAGTTGATTGGCATTCTTGATTTGCAACAAATTTCATACAAAAATGTTGATGCACGTGGATTAATCACTGGATTTCAATTTCTACAGTCTTACTACCCTGAGCGGTTAGCAAAGTGCTTCATCTTAAACATGCCATGGTTCTTTGTAAGTGTCTGGAGAATGGTTTCTCGCTTCCTTGAGAAGGCAACAGCAGAAAAGATTGTAATTGTGAGCAATGAGGATGAAACAAAAAACTTCATAAAGGAAGTTGGTGAAGAAACCTTGCCAGAAGAGTATGGTGGACGAGCAAAGCTTGTACCTCTCCAAGATGTTGTACTGGCACCAATGGAGGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CRAL_TRIO

Protein Analysis

244

Amino Acids

27.98

Weight (kDa)

8.46

Isoelectric Point (pI)

35.7

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013330)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G14820 AT1G14820 AT1G14820
fragaria_vesca FvH4_4g33460 FvH4_4g33460
malus_domestica MD13G1019700.v1.1 MD16G1017800.v1.1
prunus_persica Prupe.1G334300_v2.0.a1
pyrus_communis pycom13g01670
rosa_chinensis RchiOBHm_Chr4g0442771
rosa_laevigata RLG00000005985
rosa_roxburghii Rroxscaffold_5G00383550
rosa_rugosa Rorug04G0342900
rosa_samantha Rh4AG395500 Rh4BG408200 Rh4CG424000 Rh4DG402400
rosa_wichuraiana Rw4G034180

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 715
AccBSI CCGCTC 1 cut(s) 499
AciI CCGC 1 cut(s) 499
AclWI GGATC 1 cut(s) 318
AcsI RAATTY 2 cut(s) 329, 426
AcvI CACGTG 1 cut(s) 452
AfaI GTAC 2 cut(s) 693, 711
AfiI CCNNNNNNNGG 1 cut(s) 577
AgsI TTSAA 3 cut(s) 50, 325, 473
AjnI CCWGG 1 cut(s) 12
AjuI GAANNNNNNNTTGG 2 cut(s) 387, 419
AluBI AGCT 3 cut(s) 55, 366, 688
AluI AGCT 3 cut(s) 55, 366, 688
Alw26I GTCTC 1 cut(s) 75
AlwI GGATC 1 cut(s) 318
Ama87I CYCGRG 1 cut(s) 56
AoxI GGCC 1 cut(s) 168
ApeKI GCWGC 1 cut(s) 134
ApoI RAATTY 2 cut(s) 329, 426
AseI ATTAAT 1 cut(s) 458
Asp700I GAANNNNTTC 1 cut(s) 559
AspS9I GGNCC 2 cut(s) 121, 168
AsuC2I CCSGG 1 cut(s) 125
AsuHPI GGTGA 1 cut(s) 659
AvaI CYCGRG 1 cut(s) 56
AvaII GGWCC 1 cut(s) 121
BanI GGYRCC 1 cut(s) 715
BbrPI CACGTG 1 cut(s) 452
BbsI GAAGAC 1 cut(s) 231
BbvI GCAGC 1 cut(s) 146
BccI CCATC 1 cut(s) 254
BciT130I CCWGG 1 cut(s) 14
BciVI GTATCC 1 cut(s) 257
BcnI CCSGG 1 cut(s) 125
BcoDI GTCTC 1 cut(s) 75
BfmI CTRYAG 1 cut(s) 479
BfuI GTATCC 1 cut(s) 257
BisI GCNGC 1 cut(s) 135
BlsI GCNGC 1 cut(s) 136
Bme1390I CCNGG 2 cut(s) 14, 125
Bme18I GGWCC 1 cut(s) 121
BmeT110I CYCGRG 1 cut(s) 56
BmgT120I GGNCC 2 cut(s) 121, 168
BmiI GGNNCC 2 cut(s) 123, 717
BmrFI CCNGG 2 cut(s) 14, 125
BmsI GCATC 2 cut(s) 205, 436
BpiI GAAGAC 1 cut(s) 231
BpmI CTGGAG 1 cut(s) 571
Bpu10I CCTNAGC 1 cut(s) 495
BpuEI CTTGAG 1 cut(s) 593
BpuMI CCSGG 1 cut(s) 125
BsaAI YACGTR 1 cut(s) 452
BsaI GGTCTC 1 cut(s) 75
BsaJI CCNNGG 1 cut(s) 530
Bsc4I CCNNNNNNNGG 1 cut(s) 577
Bse1I ACTGG 2 cut(s) 469, 717
Bse3DI GCAATG 1 cut(s) 616
BseBI CCWGG 1 cut(s) 14
BseDI CCNNGG 1 cut(s) 530
BseGI GGATG 2 cut(s) 265, 622
BseLI CCNNNNNNNGG 1 cut(s) 577
BseMI GCAATG 1 cut(s) 616
BseMII CTCAG 2 cut(s) 21, 486
BseNI ACTGG 2 cut(s) 469, 717
BseXI GCAGC 1 cut(s) 146
BshFI GGCC 1 cut(s) 170
BshNI GGYRCC 1 cut(s) 715
BsiHKCI CYCGRG 1 cut(s) 56
BsiSI CCGG 1 cut(s) 125
BslFI GGGAC 1 cut(s) 73
BslI CCNNNNNNNGG 1 cut(s) 577
BsmAI GTCTC 1 cut(s) 75
BsmFI GGGAC 1 cut(s) 73
BsmI GAATGC 1 cut(s) 408
BsnI GGCC 1 cut(s) 170
Bso31I GGTCTC 1 cut(s) 75
BsoBI CYCGRG 1 cut(s) 56
Bsp143I GATC 2 cut(s) 113, 310
Bsp19I CCATGG 1 cut(s) 530
BspACI CCGC 1 cut(s) 499
BspANI GGCC 1 cut(s) 170
BspCNI CTCAG 2 cut(s) 22, 487
BspHI TCATGA 1 cut(s) 93
BspLI GGNNCC 2 cut(s) 123, 717
BspPI GGATC 1 cut(s) 318
BspT107I GGYRCC 1 cut(s) 715
BspTNI GGTCTC 1 cut(s) 75
BsrBI CCGCTC 1 cut(s) 499
BsrDI GCAATG 1 cut(s) 616
BsrI ACTGG 2 cut(s) 469, 717
BssECI CCNNGG 1 cut(s) 530
BssMI GATC 2 cut(s) 113, 310
BssT1I CCWWGG 1 cut(s) 530
Bst2UI CCWGG 1 cut(s) 14
Bst4CI ACNGT 1 cut(s) 483
Bst6I CTCTTC 1 cut(s) 660
BstBAI YACGTR 1 cut(s) 452
BstC8I GCNNGC 3 cut(s) 289, 293, 364
BstDEI CTNAG 4 cut(s) 30, 255, 306, 495
BstDSI CCRYGG 1 cut(s) 530
BstENI CCTNNNNNAGG 1 cut(s) 575
BstF5I GGATG 2 cut(s) 265, 622
BstKTI GATC 2 cut(s) 116, 313
BstMAI GTCTC 1 cut(s) 75
BstMBI GATC 2 cut(s) 113, 310
BstNI CCWGG 1 cut(s) 14
BstNSI RCATGY 1 cut(s) 529
BstSCI CCNGG 2 cut(s) 12, 123
BstSFI CTRYAG 1 cut(s) 479
BstV1I GCAGC 1 cut(s) 146
BstV2I GAAGAC 1 cut(s) 231
BsuI GTATCC 1 cut(s) 257
BsuRI GGCC 1 cut(s) 170
BtgI CCRYGG 1 cut(s) 530
BtsCI GGATG 2 cut(s) 265, 622
BtsIMutI CAGTG 3 cut(s) 23, 158, 462
Cac8I GCNNGC 3 cut(s) 289, 293, 364
CciI TCATGA 1 cut(s) 93
Cfr13I GGNCC 2 cut(s) 121, 168
Csp6I GTAC 2 cut(s) 692, 710
CviAII CATG 3 cut(s) 94, 526, 531
CviJI RGCY 4 cut(s) 55, 170, 366, 688
CviKI_1 RGCY 4 cut(s) 55, 170, 366, 688
CviQI GTAC 2 cut(s) 692, 710
DdeI CTNAG 4 cut(s) 30, 255, 306, 495
DpnI GATC 2 cut(s) 115, 312
DpnII GATC 2 cut(s) 113, 310
Eam1104I CTCTTC 1 cut(s) 660
EarI CTCTTC 1 cut(s) 660
Eco130I CCWWGG 1 cut(s) 530
Eco31I GGTCTC 1 cut(s) 75
Eco47I GGWCC 1 cut(s) 121
Eco72I CACGTG 1 cut(s) 452
Eco88I CYCGRG 1 cut(s) 56
EcoNI CCTNNNNNAGG 1 cut(s) 575
EcoO109I RGGNCCY 1 cut(s) 168
EcoRII CCWGG 1 cut(s) 12
EcoT14I CCWWGG 1 cut(s) 530
EcoT22I ATGCAT 1 cut(s) 220
ErhI CCWWGG 1 cut(s) 530
FaeI CATG 3 cut(s) 97, 529, 534
FaiI YATR 8 cut(s) 78, 95, 159, 433, 527, 532, 635, 672
FaqI GGGAC 1 cut(s) 73
FatI CATG 3 cut(s) 93, 525, 530
Fnu4HI GCNGC 1 cut(s) 135
FokI GGATG 2 cut(s) 272, 629
Fsp4HI GCNGC 1 cut(s) 135
GluI GCNGC 1 cut(s) 135
GsuI CTGGAG 1 cut(s) 571
HaeIII GGCC 1 cut(s) 170
HapII CCGG 1 cut(s) 125
Hin1II CATG 3 cut(s) 97, 529, 534
HindIII AAGCTT 1 cut(s) 686
HinfI GANTC 1 cut(s) 200
HpaII CCGG 1 cut(s) 125
HphI GGTGA 1 cut(s) 659
Hpy166II GTNNAC 1 cut(s) 677
Hpy188I TCNGA 3 cut(s) 199, 205, 374
Hpy188III TCNNGA 6 cut(s) 58, 94, 103, 325, 413, 550
Hpy8I GTNNAC 1 cut(s) 677
HpyAV CCTTC 2 cut(s) 312, 571
HpyCH4III ACNGT 1 cut(s) 483
HpyCH4IV ACGT 1 cut(s) 451
HpyCH4V TGCA 7 cut(s) 23, 110, 218, 244, 362, 421, 449
HpyF3I CTNAG 4 cut(s) 30, 255, 306, 495
HpySE526I ACGT 1 cut(s) 451
Hsp92II CATG 3 cut(s) 97, 529, 534
Kzo9I GATC 2 cut(s) 113, 310
LpnPI CCDG 8 cut(s) 26, 138, 230, 450, 507, 535, 675, 698
Lsp1109I GCAGC 1 cut(s) 146
LweI GCATC 2 cut(s) 205, 436
MaeII ACGT 1 cut(s) 451
MalI GATC 2 cut(s) 115, 312
MbiI CCGCTC 1 cut(s) 499
MboI GATC 2 cut(s) 113, 310
MboII GAAGA 3 cut(s) 236, 662, 677
MfeI CAATTG 1 cut(s) 357
MluCI AATT 8 cut(s) 18, 320, 329, 357, 384, 426, 473, 600
MnlI CCTC 8 cut(s) 30, 90, 159, 181, 368, 607, 705, 718
Mph1103I ATGCAT 1 cut(s) 220
MroXI GAANNNNTTC 1 cut(s) 559
MseI TTAA 2 cut(s) 458, 521
MspI CCGG 1 cut(s) 125
MspR9I CCNGG 2 cut(s) 14, 125
MunI CAATTG 1 cut(s) 357
Mva1269I GAATGC 1 cut(s) 408
MvaI CCWGG 1 cut(s) 14
NciI CCSGG 1 cut(s) 125
NcoI CCATGG 1 cut(s) 530
NdeII GATC 2 cut(s) 113, 310
NlaIII CATG 3 cut(s) 97, 529, 534
NlaIV GGNNCC 2 cut(s) 123, 717
NsiI ATGCAT 1 cut(s) 220
NspI RCATGY 1 cut(s) 529
PagI TCATGA 1 cut(s) 93
PctI GAATGC 1 cut(s) 408
PdmI GAANNNNTTC 1 cut(s) 559
PfeI GAWTC 1 cut(s) 200
PkrI GCNGC 1 cut(s) 136
PmaCI CACGTG 1 cut(s) 452
PmlI CACGTG 1 cut(s) 452
Ppu21I YACGTR 1 cut(s) 452
PshBI ATTAAT 1 cut(s) 458
Psp6I CCWGG 1 cut(s) 12
PspCI CACGTG 1 cut(s) 452
PspGI CCWGG 1 cut(s) 12
PspN4I GGNNCC 2 cut(s) 123, 717
PspPI GGNCC 2 cut(s) 121, 168
RsaI GTAC 2 cut(s) 693, 711
RsaNI GTAC 2 cut(s) 692, 710
SaqAI TTAA 2 cut(s) 458, 521
SatI GCNGC 1 cut(s) 135
Sau3AI GATC 2 cut(s) 113, 310
Sau96I GGNCC 2 cut(s) 121, 168
ScrFI CCNGG 2 cut(s) 14, 125
SetI ASST 7 cut(s) 57, 101, 368, 454, 659, 690, 697
SfaNI GCATC 2 cut(s) 205, 436
SfcI CTRYAG 1 cut(s) 479
SinI GGWCC 1 cut(s) 121
SmlI CTYRAG 1 cut(s) 572
SmoI CTYRAG 1 cut(s) 572
Sse9I AATT 8 cut(s) 18, 320, 329, 357, 384, 426, 473, 600
SsiI CCGC 1 cut(s) 499
StyD4I CCNGG 2 cut(s) 12, 123
StyI CCWWGG 1 cut(s) 530
TaaI ACNGT 1 cut(s) 483
TaiI ACGT 1 cut(s) 454
TasI AATT 8 cut(s) 18, 320, 329, 357, 384, 426, 473, 600
TatI WGTACW 1 cut(s) 709
TfiI GAWTC 1 cut(s) 200
Tru1I TTAA 2 cut(s) 458, 521
Tru9I TTAA 2 cut(s) 458, 521
TscAI CASTG 3 cut(s) 30, 158, 469
TseI GCWGC 1 cut(s) 134
TspDTI ATGAA 7 cut(s) 181, 329, 408, 420, 505, 622, 633
TspRI CASTG 3 cut(s) 30, 158, 469
VpaK11BI GGWCC 1 cut(s) 121
VspI ATTAAT 1 cut(s) 458
XagI CCTNNNNNAGG 1 cut(s) 575
XapI RAATTY 2 cut(s) 329, 426
XceI RCATGY 1 cut(s) 529
XmnI GAANNNNTTC 1 cut(s) 559
Zsp2I ATGCAT 1 cut(s) 220
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.