MD16G1017800.v1.1

CRAL/TRIO domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0013330
Physical Location & Seq
Reverse (-)
1301307 .. 1303651
2345 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1017800.v1.1.491

Sequence Viewer

Length: 732 bp
ATGGAGAATACCCAAGATTTTGCTCTGGCTGAGATGAAGAAATCAGTGCAAAAGCTTGGATCTTCCACTGAGAAGTTTGGAGACCCAACTCTCATGAGGTTCTTGATTGCGAGATCAATGGACCCGGACAAAGCAGCAAAGATGTTTGTTCAGTGGCACAAATGGAGGGCTTCGTTTGTTCCGAATGGATTCGTTTCAGATGGCGAAGTGAAGGATCAATTGGCAGACAGAAAGATCTTCTTGCAGGGTTTGTCCAAGGACGGGTACCCTGTGATGGTTGTGAAGGCAAGCAAGCATTGTCCTTCCAAGGACCAGCTCCAATTCAAGAAATTCGTGGTTCATCTGCTTGACAAAACAATTGCAAGCTCTTTCAGGGGAAAAGAAATTGGAAACGAAAAGATGATCGTCATTCTTGATTTGCAACAACTTTCATACAAAAATGTTGATGCGCGAGGGTTTATCACCGGATTTCAATTTCTGCAGTCTTACTACCCCGAGCGATTAGCGAAGTTGTTCATCTTAAGCATGCCATGGTTTTTCGTAAGTGTCTGGAGGATGATTTCTCGCTTCCTCGAGAAGGCAACGCAAGAGAAGATTGTGATTGTGAGCAATGAGGAAGGAACAAAAAACTTCAGAAAGGAAATCGGCGAAGAAACCTTGCCAGAAGAGTATGGCGGACGAGCAAAGCTTGTACTTCTCCAAGATGTTGTACTGGCACCGGTGGAGGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CRAL_TRIO

Protein Analysis

244

Amino Acids

27.9

Weight (kDa)

9.11

Isoelectric Point (pI)

37.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CRAL_TRIO PF00650 77 - 226 9.1e-31 CRAL/TRIO domain
CRAL_TRIO_2 PF13716 88 - 226 2.5e-08 Divergent CRAL/TRIO domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0013330)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G14820 AT1G14820 AT1G14820
fragaria_vesca FvH4_4g33460 FvH4_4g33460
malus_domestica MD13G1019700.v1.1 MD16G1017800.v1.1
prunus_persica Prupe.1G334300_v2.0.a1
pyrus_communis pycom13g01670
rosa_chinensis RchiOBHm_Chr4g0442771
rosa_laevigata RLG00000005985
rosa_roxburghii Rroxscaffold_5G00383550
rosa_rugosa Rorug04G0342900
rosa_samantha Rh4AG395500 Rh4BG408200 Rh4CG424000 Rh4DG402400
rosa_wichuraiana Rw4G034180

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 264
AccB1I GGYRCC 2 cut(s) 264, 715
AccII CGCG 1 cut(s) 451
AciI CCGC 1 cut(s) 675
AclWI GGATC 2 cut(s) 67, 222
AcsI RAATTY 1 cut(s) 329
AcuI CTGAAG 1 cut(s) 616
AfaI GTAC 3 cut(s) 266, 693, 711
AfiI CCNNNNNNNGG 3 cut(s) 261, 274, 577
AflII CTTAAG 1 cut(s) 520
AgeI ACCGGT 1 cut(s) 718
AgsI TTSAA 2 cut(s) 325, 473
AjuI GAANNNNNNNTTGG 2 cut(s) 203, 235
AluBI AGCT 4 cut(s) 55, 316, 366, 688
AluI AGCT 4 cut(s) 55, 316, 366, 688
Alw26I GTCTC 1 cut(s) 75
AlwI GGATC 2 cut(s) 67, 222
Ama87I CYCGRG 2 cut(s) 494, 572
ApeKI GCWGC 1 cut(s) 134
ApoI RAATTY 1 cut(s) 329
AsiGI ACCGGT 1 cut(s) 718
Asp700I GAANNNNTTC 2 cut(s) 188, 512
Asp718I GGTACC 1 cut(s) 264
AspLEI GCGC 1 cut(s) 451
AspS9I GGNCC 2 cut(s) 121, 310
AsuC2I CCSGG 1 cut(s) 125
AsuHPI GGTGA 1 cut(s) 454
AvaI CYCGRG 2 cut(s) 494, 572
AvaII GGWCC 2 cut(s) 121, 310
BanI GGYRCC 2 cut(s) 264, 715
BbvI GCAGC 1 cut(s) 146
BccI CCATC 2 cut(s) 194, 268
BcnI CCSGG 1 cut(s) 125
BcoDI GTCTC 1 cut(s) 75
BfmI CTRYAG 1 cut(s) 479
BfrI CTTAAG 1 cut(s) 520
BglII AGATCT 1 cut(s) 234
BisI GCNGC 1 cut(s) 135
BlsI GCNGC 1 cut(s) 136
Bme1390I CCNGG 1 cut(s) 125
Bme18I GGWCC 2 cut(s) 121, 310
BmeT110I CYCGRG 2 cut(s) 494, 572
BmgT120I GGNCC 2 cut(s) 121, 310
BmiI GGNNCC 3 cut(s) 123, 266, 717
BmrFI CCNGG 1 cut(s) 125
BmsI GCATC 1 cut(s) 436
BpmI CTGGAG 1 cut(s) 571
BpuMI CCSGG 1 cut(s) 125
BsaI GGTCTC 1 cut(s) 75
BsaJI CCNNGG 3 cut(s) 255, 306, 530
BsaWI WCCGGW 2 cut(s) 464, 718
Bsc4I CCNNNNNNNGG 3 cut(s) 261, 274, 577
Bse118I RCCGGY 1 cut(s) 718
Bse1I ACTGG 1 cut(s) 717
Bse3DI GCAATG 1 cut(s) 616
BseDI CCNNGG 3 cut(s) 255, 306, 530
BseGI GGATG 1 cut(s) 561
BseLI CCNNNNNNNGG 3 cut(s) 261, 274, 577
BseMI GCAATG 1 cut(s) 616
BseMII CTCAG 2 cut(s) 21, 60
BseNI ACTGG 1 cut(s) 717
BseXI GCAGC 1 cut(s) 146
Bsh1236I CGCG 1 cut(s) 451
BshNI GGYRCC 2 cut(s) 264, 715
BshTI ACCGGT 1 cut(s) 718
BsiHKCI CYCGRG 2 cut(s) 494, 572
BsiSI CCGG 3 cut(s) 125, 465, 719
BslI CCNNNNNNNGG 3 cut(s) 261, 274, 577
BsmAI GTCTC 1 cut(s) 75
Bso31I GGTCTC 1 cut(s) 75
BsoBI CYCGRG 2 cut(s) 494, 572
Bsp143I GATC 5 cut(s) 59, 113, 214, 234, 402
Bsp19I CCATGG 1 cut(s) 530
BspACI CCGC 1 cut(s) 675
BspCNI CTCAG 2 cut(s) 22, 61
BspFNI CGCG 1 cut(s) 451
BspHI TCATGA 1 cut(s) 93
BspLI GGNNCC 3 cut(s) 123, 266, 717
BspMAI CTGCAG 1 cut(s) 483
BspPI GGATC 2 cut(s) 67, 222
BspT107I GGYRCC 2 cut(s) 264, 715
BspTI CTTAAG 1 cut(s) 520
BspTNI GGTCTC 1 cut(s) 75
BsrDI GCAATG 1 cut(s) 616
BsrFI RCCGGY 1 cut(s) 718
BsrI ACTGG 1 cut(s) 717
BssAI RCCGGY 1 cut(s) 718
BssECI CCNNGG 3 cut(s) 255, 306, 530
BssMI GATC 5 cut(s) 59, 113, 214, 234, 402
BssT1I CCWWGG 3 cut(s) 255, 306, 530
Bst6I CTCTTC 1 cut(s) 660
BstAFI CTTAAG 1 cut(s) 520
BstC8I GCNNGC 4 cut(s) 289, 293, 364, 527
BstDEI CTNAG 2 cut(s) 30, 69
BstDSI CCRYGG 1 cut(s) 530
BstENI CCTNNNNNAGG 1 cut(s) 575
BstF5I GGATG 1 cut(s) 561
BstFNI CGCG 1 cut(s) 451
BstHHI GCGC 1 cut(s) 451
BstKTI GATC 5 cut(s) 62, 116, 217, 237, 405
BstMAI GTCTC 1 cut(s) 75
BstMBI GATC 5 cut(s) 59, 113, 214, 234, 402
BstNSI RCATGY 1 cut(s) 529
BstSCI CCNGG 1 cut(s) 123
BstSFI CTRYAG 1 cut(s) 479
BstUI CGCG 1 cut(s) 451
BstV1I GCAGC 1 cut(s) 146
BstX2I RGATCY 2 cut(s) 59, 234
BstYI RGATCY 2 cut(s) 59, 234
BtgI CCRYGG 1 cut(s) 530
BtsCI GGATG 1 cut(s) 561
BtsIMutI CAGTG 3 cut(s) 51, 66, 158
Cac8I GCNNGC 4 cut(s) 289, 293, 364, 527
CciI TCATGA 1 cut(s) 93
CfoI GCGC 1 cut(s) 451
Cfr10I RCCGGY 1 cut(s) 718
Cfr13I GGNCC 2 cut(s) 121, 310
Csp6I GTAC 3 cut(s) 265, 692, 710
CspAI ACCGGT 1 cut(s) 718
CviAII CATG 3 cut(s) 94, 526, 531
CviJI RGCY 6 cut(s) 29, 55, 170, 316, 366, 688
CviKI_1 RGCY 6 cut(s) 29, 55, 170, 316, 366, 688
CviQI GTAC 3 cut(s) 265, 692, 710
DdeI CTNAG 2 cut(s) 30, 69
DpnI GATC 5 cut(s) 61, 115, 216, 236, 404
DpnII GATC 5 cut(s) 59, 113, 214, 234, 402
Eam1104I CTCTTC 1 cut(s) 660
EarI CTCTTC 1 cut(s) 660
EciI GGCGGA 1 cut(s) 690
Eco130I CCWWGG 3 cut(s) 255, 306, 530
Eco31I GGTCTC 1 cut(s) 75
Eco47I GGWCC 2 cut(s) 121, 310
Eco57I CTGAAG 1 cut(s) 616
Eco88I CYCGRG 2 cut(s) 494, 572
EcoNI CCTNNNNNAGG 1 cut(s) 575
EcoT14I CCWWGG 3 cut(s) 255, 306, 530
ErhI CCWWGG 3 cut(s) 255, 306, 530
FaeI CATG 3 cut(s) 97, 529, 534
FaiI YATR 5 cut(s) 95, 433, 527, 532, 672
FalI AAGNNNNNCTT 2 cut(s) 224, 256
FatI CATG 3 cut(s) 93, 525, 530
Fnu4HI GCNGC 1 cut(s) 135
FokI GGATG 1 cut(s) 568
Fsp4HI GCNGC 1 cut(s) 135
GlaI GCGC 1 cut(s) 450
GluI GCNGC 1 cut(s) 135
GsuI CTGGAG 1 cut(s) 571
HapII CCGG 3 cut(s) 125, 465, 719
HhaI GCGC 1 cut(s) 451
Hin1II CATG 3 cut(s) 97, 529, 534
Hin6I GCGC 1 cut(s) 449
HinP1I GCGC 1 cut(s) 449
HindIII AAGCTT 2 cut(s) 53, 686
HinfI GANTC 1 cut(s) 189
HpaII CCGG 3 cut(s) 125, 465, 719
HphI GGTGA 1 cut(s) 454
Hpy188I TCNGA 3 cut(s) 183, 199, 635
Hpy188III TCNNGA 6 cut(s) 94, 103, 325, 413, 550, 574
HpyAV CCTTC 5 cut(s) 205, 277, 312, 571, 611
HpyCH4V TGCA 5 cut(s) 49, 244, 362, 421, 481
HpyF3I CTNAG 2 cut(s) 30, 69
Hsp92II CATG 3 cut(s) 97, 529, 534
HspAI GCGC 1 cut(s) 449
KpnI GGTACC 1 cut(s) 268
Kzo9I GATC 5 cut(s) 59, 113, 214, 234, 402
LmnI GCTCC 1 cut(s) 321
Lsp1109I GCAGC 1 cut(s) 146
LweI GCATC 1 cut(s) 436
MalI GATC 5 cut(s) 61, 115, 216, 236, 404
MboI GATC 5 cut(s) 59, 113, 214, 234, 402
MboII GAAGA 6 cut(s) 49, 54, 229, 604, 662, 677
MfeI CAATTG 2 cut(s) 218, 357
MflI RGATCY 2 cut(s) 59, 234
MluCI AATT 6 cut(s) 218, 320, 329, 357, 384, 473
MnlI CCTC 7 cut(s) 90, 159, 446, 546, 581, 607, 718
MroXI GAANNNNTTC 2 cut(s) 188, 512
MseI TTAA 1 cut(s) 521
MspCI CTTAAG 1 cut(s) 520
MspI CCGG 3 cut(s) 125, 465, 719
MspR9I CCNGG 1 cut(s) 125
MunI CAATTG 2 cut(s) 218, 357
MvnI CGCG 1 cut(s) 451
NciI CCSGG 1 cut(s) 125
NcoI CCATGG 1 cut(s) 530
NdeII GATC 5 cut(s) 59, 113, 214, 234, 402
NlaIII CATG 3 cut(s) 97, 529, 534
NlaIV GGNNCC 3 cut(s) 123, 266, 717
NspI RCATGY 1 cut(s) 529
PaeI GCATGC 1 cut(s) 529
PaeR7I CTCGAG 1 cut(s) 572
PagI TCATGA 1 cut(s) 93
PcsI WCGNNNNNNNCGW 1 cut(s) 179
PdmI GAANNNNTTC 2 cut(s) 188, 512
PfeI GAWTC 1 cut(s) 189
PinAI ACCGGT 1 cut(s) 718
PkrI GCNGC 1 cut(s) 136
PspN4I GGNNCC 3 cut(s) 123, 266, 717
PspPI GGNCC 2 cut(s) 121, 310
PstI CTGCAG 1 cut(s) 483
PsuI RGATCY 2 cut(s) 59, 234
RsaI GTAC 3 cut(s) 266, 693, 711
RsaNI GTAC 3 cut(s) 265, 692, 710
SaqAI TTAA 1 cut(s) 521
SatI GCNGC 1 cut(s) 135
Sau3AI GATC 5 cut(s) 59, 113, 214, 234, 402
Sau96I GGNCC 2 cut(s) 121, 310
ScrFI CCNGG 1 cut(s) 125
SetI ASST 6 cut(s) 57, 101, 318, 368, 659, 690
SfaNI GCATC 1 cut(s) 436
SfcI CTRYAG 1 cut(s) 479
Sfr274I CTCGAG 1 cut(s) 572
SgrAI CRCCGGYG 1 cut(s) 718
SinI GGWCC 2 cut(s) 121, 310
SlaI CTCGAG 1 cut(s) 572
SmlI CTYRAG 2 cut(s) 520, 572
SmoI CTYRAG 2 cut(s) 520, 572
SphI GCATGC 1 cut(s) 529
Sse9I AATT 6 cut(s) 218, 320, 329, 357, 384, 473
SsiI CCGC 1 cut(s) 675
StyD4I CCNGG 1 cut(s) 123
StyI CCWWGG 3 cut(s) 255, 306, 530
TaqI TCGA 1 cut(s) 573
TasI AATT 6 cut(s) 218, 320, 329, 357, 384, 473
TatI WGTACW 2 cut(s) 691, 709
TfiI GAWTC 1 cut(s) 189
Tru1I TTAA 1 cut(s) 521
Tru9I TTAA 1 cut(s) 521
TscAI CASTG 3 cut(s) 51, 73, 158
TseI GCWGC 1 cut(s) 134
TspDTI ATGAA 4 cut(s) 50, 329, 420, 505
TspRI CASTG 3 cut(s) 51, 73, 158
Vha464I CTTAAG 1 cut(s) 520
VpaK11BI GGWCC 2 cut(s) 121, 310
XagI CCTNNNNNAGG 1 cut(s) 575
XapI RAATTY 1 cut(s) 329
XceI RCATGY 1 cut(s) 529
XhoI CTCGAG 1 cut(s) 572
XmnI GAANNNNTTC 2 cut(s) 188, 512
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.