Rw4G034180

CRAL/TRIO domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0013330
Physical Location & Seq
Reverse (-)
61262330 .. 61265243
2914 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G034180.1

Sequence Viewer

Length: 729 bp
ATGGAGAAAACCCAGGAAGTTGCTGTGACCCAGATGAGGACATTAGTTCAGAAGCTTGGGTCGTCCACTGAGAAGTATGGAGACCCAACACTGATGAGGTTCTTGATTGCAAGATCAATGGACCCAGAGAAGGCAGCAAAGATGTTTGTGCAGTGGCGGAAATGGAGGGCTTCATTTGTCCCAAATGGGTTCATTTCGGAATCCGAAGTTAAGGATCAATTGGAAGACAGAAAGATTTTCTTGCAGGATTTATCTAATGGATGTCCTGTGATGATTATTAAAGCAAGCAAGCATTATCCTGCCAAGGACCAACTCCAATTCAAGAAATTTGTGGTTCATCTGCTTGACAAAACTATTGCAAGCTCTTTCAGAGGAAGAGAAATCGGAAACGAAAGGTTGATTGGCATTCTTGATTTGCAACAAATTTCCTATAAAAATGTTGATGCACGTGGATTAATCACTGGATTTCAATTTCTACAGGCTTACTACCCTGAAAGATTAGGAAAGCTCTTCATTCTAAACATGCCACGCTTTTTTGTAAGTGTCTGGAGGATGGTTTCTTGCTTCCTTGAGAAGGCAACTGCAGCAAAGGTTGTAATTGTGACCAATGAAGATGAAAAAAGAGACTTCATAAAGGAAATAGGCGAGGAAGTCTTGCCGGAAGAGTATGGTGGTCGAGCAAAGCTTGTAGCTATTCAAGACGTTGAACTGGCCCCAGTAGAGGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CRAL_TRIO

Protein Analysis

242

Amino Acids

27.83

Weight (kDa)

8.72

Isoelectric Point (pI)

37.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CRAL_TRIO PF00650 85 - 225 4.2e-26 CRAL/TRIO domain
CRAL_TRIO_2 PF13716 87 - 224 2.2e-06 Divergent CRAL/TRIO domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013330)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G14820 AT1G14820 AT1G14820
fragaria_vesca FvH4_4g33460 FvH4_4g33460
malus_domestica MD13G1019700.v1.1 MD16G1017800.v1.1
prunus_persica Prupe.1G334300_v2.0.a1
pyrus_communis pycom13g01670
rosa_chinensis RchiOBHm_Chr4g0442771
rosa_laevigata RLG00000005985
rosa_roxburghii Rroxscaffold_5G00383550
rosa_rugosa Rorug04G0342900
rosa_samantha Rh4AG395500 Rh4BG408200 Rh4CG424000 Rh4DG402400
rosa_wichuraiana Rw4G034180

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 157
AclWI GGATC 1 cut(s) 222
AcsI RAATTY 2 cut(s) 326, 423
AcvI CACGTG 1 cut(s) 449
AfiI CCNNNNNNNGG 4 cut(s) 36, 130, 574, 721
AgsI TTSAA 4 cut(s) 322, 470, 698, 707
AjnI CCWGG 1 cut(s) 12
AjuI GAANNNNNNNTTGG 2 cut(s) 384, 416
AluBI AGCT 5 cut(s) 55, 363, 508, 685, 692
AluI AGCT 5 cut(s) 55, 363, 508, 685, 692
Alw26I GTCTC 2 cut(s) 75, 618
AlwI GGATC 1 cut(s) 222
AoxI GGCC 1 cut(s) 711
ApeKI GCWGC 2 cut(s) 134, 584
ApoI RAATTY 2 cut(s) 326, 423
AseI ATTAAT 1 cut(s) 455
AspS9I GGNCC 3 cut(s) 121, 307, 712
AvaII GGWCC 2 cut(s) 121, 307
BbrPI CACGTG 1 cut(s) 449
BbsI GAAGAC 1 cut(s) 231
BbvI GCAGC 2 cut(s) 146, 596
BccI CCATC 1 cut(s) 547
BciT130I CCWGG 1 cut(s) 14
BcoDI GTCTC 2 cut(s) 75, 618
BfmI CTRYAG 2 cut(s) 476, 582
BisI GCNGC 2 cut(s) 135, 585
BlsI GCNGC 2 cut(s) 136, 586
Bme1390I CCNGG 1 cut(s) 14
Bme18I GGWCC 2 cut(s) 121, 307
BmgT120I GGNCC 3 cut(s) 121, 307, 712
BmiI GGNNCC 2 cut(s) 123, 714
BmrFI CCNGG 1 cut(s) 14
BmrI ACTGGG 1 cut(s) 710
BmsI GCATC 1 cut(s) 433
BmuI ACTGGG 1 cut(s) 710
BpiI GAAGAC 1 cut(s) 231
BpmI CTGGAG 1 cut(s) 568
BpuEI CTTGAG 1 cut(s) 590
BsaAI YACGTR 1 cut(s) 449
BsaI GGTCTC 1 cut(s) 75
BsaJI CCNNGG 2 cut(s) 12, 303
Bsc4I CCNNNNNNNGG 4 cut(s) 36, 130, 574, 721
Bse1I ACTGG 3 cut(s) 466, 714, 716
BseBI CCWGG 1 cut(s) 14
BseDI CCNNGG 2 cut(s) 12, 303
BseGI GGATG 2 cut(s) 266, 558
BseLI CCNNNNNNNGG 4 cut(s) 36, 130, 574, 721
BseMII CTCAG 1 cut(s) 60
BseNI ACTGG 3 cut(s) 466, 714, 716
BseXI GCAGC 2 cut(s) 146, 596
BsgI GTGCAG 1 cut(s) 170
BshFI GGCC 1 cut(s) 713
BsiSI CCGG 1 cut(s) 659
BslFI GGGAC 1 cut(s) 164
BslI CCNNNNNNNGG 4 cut(s) 36, 130, 574, 721
BsmAI GTCTC 2 cut(s) 75, 618
BsmFI GGGAC 1 cut(s) 164
BsmI GAATGC 1 cut(s) 405
BsnI GGCC 1 cut(s) 713
Bso31I GGTCTC 1 cut(s) 75
Bsp143I GATC 2 cut(s) 113, 214
BspACI CCGC 1 cut(s) 157
BspANI GGCC 1 cut(s) 713
BspCNI CTCAG 1 cut(s) 61
BspLI GGNNCC 2 cut(s) 123, 714
BspMAI CTGCAG 1 cut(s) 586
BspPI GGATC 1 cut(s) 222
BspQI GCTCTTC 1 cut(s) 515
BspTNI GGTCTC 1 cut(s) 75
BsrI ACTGG 3 cut(s) 466, 714, 716
BssECI CCNNGG 2 cut(s) 12, 303
BssMI GATC 2 cut(s) 113, 214
BssT1I CCWWGG 1 cut(s) 303
Bst2UI CCWGG 1 cut(s) 14
Bst6I CTCTTC 3 cut(s) 370, 515, 657
BstBAI YACGTR 1 cut(s) 449
BstC8I GCNNGC 3 cut(s) 286, 290, 361
BstDEI CTNAG 1 cut(s) 69
BstENI CCTNNNNNAGG 1 cut(s) 572
BstF5I GGATG 2 cut(s) 266, 558
BstKTI GATC 2 cut(s) 116, 217
BstMAI GTCTC 2 cut(s) 75, 618
BstMBI GATC 2 cut(s) 113, 214
BstMWI GCNNNNNNNGC 1 cut(s) 584
BstNI CCWGG 1 cut(s) 14
BstNSI RCATGY 1 cut(s) 526
BstSCI CCNGG 1 cut(s) 12
BstSFI CTRYAG 2 cut(s) 476, 582
BstV1I GCAGC 2 cut(s) 146, 596
BstV2I GAAGAC 1 cut(s) 231
BsuRI GGCC 1 cut(s) 713
BtsCI GGATG 2 cut(s) 266, 558
BtsI GCAGTG 1 cut(s) 158
BtsIMutI CAGTG 4 cut(s) 66, 89, 158, 459
Cac8I GCNNGC 3 cut(s) 286, 290, 361
Cfr13I GGNCC 3 cut(s) 121, 307, 712
CviAII CATG 1 cut(s) 523
CviJI RGCY 8 cut(s) 55, 170, 363, 482, 508, 685, 692, 713
CviKI_1 RGCY 8 cut(s) 55, 170, 363, 482, 508, 685, 692, 713
DdeI CTNAG 1 cut(s) 69
DpnI GATC 2 cut(s) 115, 216
DpnII GATC 2 cut(s) 113, 214
Eam1104I CTCTTC 3 cut(s) 370, 515, 657
EarI CTCTTC 3 cut(s) 370, 515, 657
EciI GGCGGA 1 cut(s) 172
Eco130I CCWWGG 1 cut(s) 303
Eco31I GGTCTC 1 cut(s) 75
Eco47I GGWCC 2 cut(s) 121, 307
Eco72I CACGTG 1 cut(s) 449
EcoNI CCTNNNNNAGG 1 cut(s) 572
EcoRII CCWGG 1 cut(s) 12
EcoT14I CCWWGG 1 cut(s) 303
ErhI CCWWGG 1 cut(s) 303
FaeI CATG 1 cut(s) 526
FaiI YATR 5 cut(s) 78, 432, 524, 632, 669
FalI AAGNNNNNCTT 2 cut(s) 224, 256
FaqI GGGAC 1 cut(s) 164
FatI CATG 1 cut(s) 522
Fnu4HI GCNGC 2 cut(s) 135, 585
FokI GGATG 2 cut(s) 273, 565
Fsp4HI GCNGC 2 cut(s) 135, 585
GluI GCNGC 2 cut(s) 135, 585
GsuI CTGGAG 1 cut(s) 568
HaeIII GGCC 1 cut(s) 713
HapII CCGG 1 cut(s) 659
Hin1II CATG 1 cut(s) 526
HindIII AAGCTT 2 cut(s) 53, 683
HinfI GANTC 1 cut(s) 200
HpaII CCGG 1 cut(s) 659
Hpy166II GTNNAC 1 cut(s) 66
Hpy188I TCNGA 5 cut(s) 51, 199, 205, 371, 386
Hpy188III TCNNGA 5 cut(s) 103, 322, 410, 547, 698
Hpy8I GTNNAC 1 cut(s) 66
HpyAV CCTTC 2 cut(s) 124, 568
HpyCH4IV ACGT 2 cut(s) 448, 702
HpyCH4V TGCA 7 cut(s) 110, 151, 244, 359, 418, 446, 584
HpyF10VI GCNNNNNNNGC 1 cut(s) 584
HpyF3I CTNAG 1 cut(s) 69
HpySE526I ACGT 2 cut(s) 448, 702
Hsp92II CATG 1 cut(s) 526
Kzo9I GATC 2 cut(s) 113, 214
LguI GCTCTTC 1 cut(s) 515
Lsp1109I GCAGC 2 cut(s) 146, 596
LweI GCATC 1 cut(s) 433
MaeII ACGT 2 cut(s) 448, 702
MaeIII GTNAC 2 cut(s) 25, 601
MalI GATC 2 cut(s) 115, 216
MboI GATC 2 cut(s) 113, 214
MboII GAAGA 5 cut(s) 236, 387, 502, 623, 674
MfeI CAATTG 1 cut(s) 218
MluCI AATT 6 cut(s) 218, 317, 326, 423, 470, 597
MnlI CCTC 7 cut(s) 30, 90, 159, 365, 543, 640, 715
MseI TTAA 3 cut(s) 210, 279, 455
MspI CCGG 1 cut(s) 659
MspR9I CCNGG 1 cut(s) 14
MunI CAATTG 1 cut(s) 218
Mva1269I GAATGC 1 cut(s) 405
MvaI CCWGG 1 cut(s) 14
MwoI GCNNNNNNNGC 1 cut(s) 584
NdeII GATC 2 cut(s) 113, 214
NlaIII CATG 1 cut(s) 526
NlaIV GGNNCC 2 cut(s) 123, 714
NmuCI GTSAC 2 cut(s) 25, 601
NspI RCATGY 1 cut(s) 526
PciSI GCTCTTC 1 cut(s) 515
PctI GAATGC 1 cut(s) 405
PfeI GAWTC 1 cut(s) 200
PkrI GCNGC 2 cut(s) 136, 586
PmaCI CACGTG 1 cut(s) 449
PmlI CACGTG 1 cut(s) 449
Ppu21I YACGTR 1 cut(s) 449
PshBI ATTAAT 1 cut(s) 455
Psp6I CCWGG 1 cut(s) 12
PspCI CACGTG 1 cut(s) 449
PspGI CCWGG 1 cut(s) 12
PspN4I GGNNCC 2 cut(s) 123, 714
PspPI GGNCC 3 cut(s) 121, 307, 712
PstI CTGCAG 1 cut(s) 586
SapI GCTCTTC 1 cut(s) 515
SaqAI TTAA 3 cut(s) 210, 279, 455
SatI GCNGC 2 cut(s) 135, 585
Sau3AI GATC 2 cut(s) 113, 214
Sau96I GGNCC 3 cut(s) 121, 307, 712
ScrFI CCNGG 1 cut(s) 14
SfaNI GCATC 1 cut(s) 433
SfcI CTRYAG 2 cut(s) 476, 582
SinI GGWCC 2 cut(s) 121, 307
SmlI CTYRAG 1 cut(s) 569
SmoI CTYRAG 1 cut(s) 569
Sse9I AATT 6 cut(s) 218, 317, 326, 423, 470, 597
SsiI CCGC 1 cut(s) 157
StyD4I CCNGG 1 cut(s) 12
StyI CCWWGG 1 cut(s) 303
TaiI ACGT 2 cut(s) 451, 705
TaqI TCGA 1 cut(s) 676
TasI AATT 6 cut(s) 218, 317, 326, 423, 470, 597
TfiI GAWTC 1 cut(s) 200
Tru1I TTAA 3 cut(s) 210, 279, 455
Tru9I TTAA 3 cut(s) 210, 279, 455
TscAI CASTG 4 cut(s) 73, 96, 158, 466
TseFI GTSAC 2 cut(s) 25, 601
TseI GCWGC 2 cut(s) 134, 584
Tsp45I GTSAC 2 cut(s) 25, 601
TspDTI ATGAA 7 cut(s) 162, 181, 326, 502, 619, 624, 630
TspRI CASTG 4 cut(s) 73, 96, 158, 466
VpaK11BI GGWCC 2 cut(s) 121, 307
VspI ATTAAT 1 cut(s) 455
XagI CCTNNNNNAGG 1 cut(s) 572
XapI RAATTY 2 cut(s) 326, 423
XceI RCATGY 1 cut(s) 526
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.