pycom13g01670

CRAL/TRIO domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0013330
Physical Location & Seq
Reverse (-)
1101928 .. 1104719
2792 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g01670.4

Sequence Viewer

Length: 732 bp
ATGGAGAAACCCCAAGAAATTGCTCTGACTGAGATGAAGAAATCAGTGCAAAAGCTTGGATCTTCCACTGAGAAGTTTGGAGACCCAACACTCATGAGGTTCTTGATTGCGAGATCAATGGACTCGGACAAAGCAGCGAAGATGTTTGTTCAGTGGCACAAATGGAGGGATTCGTTTGTTCCAAATGGGTTCGTCTCGGACGCCGAAGTGAAGGATGAATTGGACGACAGAAAGATCTTCTTGCAGGGTTTGTCCAAGGACGGGTACCCTGTCATGGTGATTAAAGCAAGCAAGCATTTTCCTTCCAAGGACCAGCTCCAATTCAAGAAATTTGTGGTTCATCTGCTTGACAAAACAATTGCAAGCTCTTTCAGGGCAAAGGAAATTGGAAATGAAAAGATGATCGGCATTCTTGATTTGCAACAAATTTCATACAAAAATATTGATGCACGTGGGTTAATCACTGGATTTCAATTTCTACAGTCTTACTACCCCGAGCGATTAGCAAAGTGCTTCATTTTAAACATGCCATGGTTTTTTGTAAGTGTCTGGAGGATGGTTTCTCGCTTCCTCGAGAAGGCAACACAAGAAAAGGTTGTGATTGTGACCAGTGAGGAAGAAACGAAAAACTTCATCCGGGAAATCGGTGAAGAAACCTTGCCAGAAGAATATGGAGGACGAGCAAAGCTTGTACCTCTCCAAGATGTTATACTGGCACCATTGGAGGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CRAL_TRIO

Protein Analysis

244

Amino Acids

28.07

Weight (kDa)

7.74

Isoelectric Point (pI)

48.83

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013330)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G14820 AT1G14820 AT1G14820
fragaria_vesca FvH4_4g33460 FvH4_4g33460
malus_domestica MD13G1019700.v1.1 MD16G1017800.v1.1
prunus_persica Prupe.1G334300_v2.0.a1
pyrus_communis pycom13g01670
rosa_chinensis RchiOBHm_Chr4g0442771
rosa_laevigata RLG00000005985
rosa_roxburghii Rroxscaffold_5G00383550
rosa_rugosa Rorug04G0342900
rosa_samantha Rh4AG395500 Rh4BG408200 Rh4CG424000 Rh4DG402400
rosa_wichuraiana Rw4G034180

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 264
AccB1I GGYRCC 2 cut(s) 264, 715
AclWI GGATC 1 cut(s) 67
AcsI RAATTY 2 cut(s) 329, 426
AcvI CACGTG 1 cut(s) 452
AcyI GRCGYC 1 cut(s) 201
AfaI GTAC 2 cut(s) 266, 693
AfiI CCNNNNNNNGG 3 cut(s) 261, 274, 577
AgsI TTSAA 2 cut(s) 325, 473
AjuI GAANNNNNNNTTGG 2 cut(s) 203, 235
AluBI AGCT 4 cut(s) 55, 316, 366, 688
AluI AGCT 4 cut(s) 55, 316, 366, 688
Alw26I GTCTC 2 cut(s) 75, 199
AlwI GGATC 1 cut(s) 67
Ama87I CYCGRG 2 cut(s) 494, 572
ApeKI GCWGC 1 cut(s) 134
ApoI RAATTY 2 cut(s) 329, 426
Asp700I GAANNNNTTC 1 cut(s) 629
Asp718I GGTACC 1 cut(s) 264
AspS9I GGNCC 1 cut(s) 310
AsuC2I CCSGG 1 cut(s) 638
AsuHPI GGTGA 2 cut(s) 289, 659
AvaI CYCGRG 2 cut(s) 494, 572
AvaII GGWCC 1 cut(s) 310
BanI GGYRCC 2 cut(s) 264, 715
BbrPI CACGTG 1 cut(s) 452
BbvI GCAGC 1 cut(s) 146
BccI CCATC 1 cut(s) 550
BcnI CCSGG 1 cut(s) 638
BcoDI GTCTC 2 cut(s) 75, 199
BfmI CTRYAG 1 cut(s) 479
BglII AGATCT 1 cut(s) 234
BisI GCNGC 1 cut(s) 135
BlsI GCNGC 1 cut(s) 136
Bme1390I CCNGG 1 cut(s) 638
Bme18I GGWCC 1 cut(s) 310
BmeT110I CYCGRG 2 cut(s) 494, 572
BmgT120I GGNCC 1 cut(s) 310
BmiI GGNNCC 2 cut(s) 266, 717
BmrFI CCNGG 1 cut(s) 638
BmsI GCATC 1 cut(s) 436
BpmI CTGGAG 1 cut(s) 571
BpuMI CCSGG 1 cut(s) 638
BsaAI YACGTR 1 cut(s) 452
BsaHI GRCGYC 1 cut(s) 201
BsaI GGTCTC 1 cut(s) 75
BsaJI CCNNGG 3 cut(s) 255, 306, 530
Bsc4I CCNNNNNNNGG 3 cut(s) 261, 274, 577
Bse1I ACTGG 3 cut(s) 469, 609, 717
BseDI CCNNGG 3 cut(s) 255, 306, 530
BseGI GGATG 3 cut(s) 220, 561, 633
BseLI CCNNNNNNNGG 3 cut(s) 261, 274, 577
BseMII CTCAG 2 cut(s) 21, 60
BseNI ACTGG 3 cut(s) 469, 609, 717
BseXI GCAGC 1 cut(s) 146
BshNI GGYRCC 2 cut(s) 264, 715
BsiHKCI CYCGRG 2 cut(s) 494, 572
BsiSI CCGG 1 cut(s) 637
BslI CCNNNNNNNGG 3 cut(s) 261, 274, 577
BsmAI GTCTC 2 cut(s) 75, 199
BsmBI CGTCTC 1 cut(s) 199
BsmI GAATGC 1 cut(s) 408
Bso31I GGTCTC 1 cut(s) 75
BsoBI CYCGRG 2 cut(s) 494, 572
Bsp143I GATC 4 cut(s) 59, 113, 234, 402
Bsp19I CCATGG 1 cut(s) 530
BspCNI CTCAG 2 cut(s) 22, 61
BspHI TCATGA 1 cut(s) 93
BspLI GGNNCC 2 cut(s) 266, 717
BspPI GGATC 1 cut(s) 67
BspT107I GGYRCC 2 cut(s) 264, 715
BspTNI GGTCTC 1 cut(s) 75
BsrI ACTGG 3 cut(s) 469, 609, 717
BssECI CCNNGG 3 cut(s) 255, 306, 530
BssMI GATC 4 cut(s) 59, 113, 234, 402
BssNI GRCGYC 1 cut(s) 201
BssT1I CCWWGG 3 cut(s) 255, 306, 530
Bst4CI ACNGT 1 cut(s) 483
BstACI GRCGYC 1 cut(s) 201
BstBAI YACGTR 1 cut(s) 452
BstC8I GCNNGC 3 cut(s) 289, 293, 364
BstDEI CTNAG 2 cut(s) 30, 69
BstDSI CCRYGG 1 cut(s) 530
BstENI CCTNNNNNAGG 1 cut(s) 575
BstF5I GGATG 3 cut(s) 220, 561, 633
BstKTI GATC 4 cut(s) 62, 116, 237, 405
BstMAI GTCTC 2 cut(s) 75, 199
BstMBI GATC 4 cut(s) 59, 113, 234, 402
BstNSI RCATGY 1 cut(s) 529
BstSCI CCNGG 1 cut(s) 636
BstSFI CTRYAG 1 cut(s) 479
BstV1I GCAGC 1 cut(s) 146
BstX2I RGATCY 2 cut(s) 59, 234
BstYI RGATCY 2 cut(s) 59, 234
BtgI CCRYGG 1 cut(s) 530
BtsCI GGATG 3 cut(s) 220, 561, 633
BtsIMutI CAGTG 5 cut(s) 51, 66, 158, 462, 616
Cac8I GCNNGC 3 cut(s) 289, 293, 364
CciI TCATGA 1 cut(s) 93
Cfr13I GGNCC 1 cut(s) 310
CseI GACGC 1 cut(s) 209
Csp6I GTAC 2 cut(s) 265, 692
CviAII CATG 4 cut(s) 94, 274, 526, 531
CviJI RGCY 4 cut(s) 55, 316, 366, 688
CviKI_1 RGCY 4 cut(s) 55, 316, 366, 688
CviQI GTAC 2 cut(s) 265, 692
DdeI CTNAG 2 cut(s) 30, 69
DpnI GATC 4 cut(s) 61, 115, 236, 404
DpnII GATC 4 cut(s) 59, 113, 234, 402
DraI TTTAAA 1 cut(s) 522
Eco130I CCWWGG 3 cut(s) 255, 306, 530
Eco31I GGTCTC 1 cut(s) 75
Eco47I GGWCC 1 cut(s) 310
Eco72I CACGTG 1 cut(s) 452
Eco88I CYCGRG 2 cut(s) 494, 572
EcoNI CCTNNNNNAGG 1 cut(s) 575
EcoT14I CCWWGG 3 cut(s) 255, 306, 530
ErhI CCWWGG 3 cut(s) 255, 306, 530
Esp3I CGTCTC 1 cut(s) 199
FaeI CATG 4 cut(s) 97, 277, 529, 534
FaiI YATR 7 cut(s) 95, 275, 433, 527, 532, 672, 710
FalI AAGNNNNNCTT 2 cut(s) 224, 256
FatI CATG 4 cut(s) 93, 273, 525, 530
Fnu4HI GCNGC 1 cut(s) 135
FokI GGATG 3 cut(s) 227, 568, 620
Fsp4HI GCNGC 1 cut(s) 135
GluI GCNGC 1 cut(s) 135
GsuI CTGGAG 1 cut(s) 571
HapII CCGG 1 cut(s) 637
HgaI GACGC 1 cut(s) 209
Hin1I GRCGYC 1 cut(s) 201
Hin1II CATG 4 cut(s) 97, 277, 529, 534
HindIII AAGCTT 2 cut(s) 53, 686
HinfI GANTC 2 cut(s) 122, 170
HpaII CCGG 1 cut(s) 637
HphI GGTGA 2 cut(s) 289, 659
Hpy188I TCNGA 3 cut(s) 27, 127, 199
Hpy188III TCNNGA 6 cut(s) 94, 103, 325, 413, 550, 574
HpyAV CCTTC 3 cut(s) 205, 312, 571
HpyCH4III ACNGT 1 cut(s) 483
HpyCH4IV ACGT 1 cut(s) 451
HpyCH4V TGCA 5 cut(s) 49, 244, 362, 421, 449
HpyF3I CTNAG 2 cut(s) 30, 69
HpySE526I ACGT 1 cut(s) 451
Hsp92I GRCGYC 1 cut(s) 201
Hsp92II CATG 4 cut(s) 97, 277, 529, 534
KpnI GGTACC 1 cut(s) 268
Kzo9I GATC 4 cut(s) 59, 113, 234, 402
LmnI GCTCC 1 cut(s) 321
Lsp1109I GCAGC 1 cut(s) 146
LweI GCATC 1 cut(s) 436
MaeII ACGT 1 cut(s) 451
MaeIII GTNAC 1 cut(s) 604
MalI GATC 4 cut(s) 61, 115, 236, 404
MboI GATC 4 cut(s) 59, 113, 234, 402
MboII GAAGA 7 cut(s) 49, 54, 151, 229, 629, 662, 677
MfeI CAATTG 1 cut(s) 357
MflI RGATCY 2 cut(s) 59, 234
MluCI AATT 8 cut(s) 18, 218, 320, 329, 357, 384, 426, 473
MlyI GAGTC 1 cut(s) 116
MnlI CCTC 8 cut(s) 90, 159, 546, 581, 607, 668, 705, 718
MroXI GAANNNNTTC 1 cut(s) 629
MseI TTAA 3 cut(s) 282, 458, 521
MspI CCGG 1 cut(s) 637
MspR9I CCNGG 1 cut(s) 638
MunI CAATTG 1 cut(s) 357
Mva1269I GAATGC 1 cut(s) 408
NciI CCSGG 1 cut(s) 638
NcoI CCATGG 1 cut(s) 530
NdeII GATC 4 cut(s) 59, 113, 234, 402
NlaIII CATG 4 cut(s) 97, 277, 529, 534
NlaIV GGNNCC 2 cut(s) 266, 717
NmuCI GTSAC 1 cut(s) 604
NspI RCATGY 1 cut(s) 529
PaeR7I CTCGAG 1 cut(s) 572
PagI TCATGA 1 cut(s) 93
PctI GAATGC 1 cut(s) 408
PdmI GAANNNNTTC 1 cut(s) 629
PfeI GAWTC 1 cut(s) 170
PfoI TCCNGGA 1 cut(s) 636
PkrI GCNGC 1 cut(s) 136
PleI GAGTC 1 cut(s) 116
PmaCI CACGTG 1 cut(s) 452
PmlI CACGTG 1 cut(s) 452
PpsI GAGTC 1 cut(s) 116
Ppu21I YACGTR 1 cut(s) 452
PspCI CACGTG 1 cut(s) 452
PspN4I GGNNCC 2 cut(s) 266, 717
PspPI GGNCC 1 cut(s) 310
PsuI RGATCY 2 cut(s) 59, 234
RsaI GTAC 2 cut(s) 266, 693
RsaNI GTAC 2 cut(s) 265, 692
SaqAI TTAA 3 cut(s) 282, 458, 521
SatI GCNGC 1 cut(s) 135
Sau3AI GATC 4 cut(s) 59, 113, 234, 402
Sau96I GGNCC 1 cut(s) 310
SchI GAGTC 1 cut(s) 116
ScrFI CCNGG 1 cut(s) 638
SetI ASST 9 cut(s) 57, 101, 318, 368, 454, 597, 659, 690, 697
SfaNI GCATC 1 cut(s) 436
SfcI CTRYAG 1 cut(s) 479
Sfr274I CTCGAG 1 cut(s) 572
SinI GGWCC 1 cut(s) 310
SlaI CTCGAG 1 cut(s) 572
SmlI CTYRAG 1 cut(s) 572
SmoI CTYRAG 1 cut(s) 572
Sse9I AATT 8 cut(s) 18, 218, 320, 329, 357, 384, 426, 473
SspI AATATT 1 cut(s) 442
StyD4I CCNGG 1 cut(s) 636
StyI CCWWGG 3 cut(s) 255, 306, 530
TaaI ACNGT 1 cut(s) 483
TaiI ACGT 1 cut(s) 454
TaqI TCGA 1 cut(s) 573
TasI AATT 8 cut(s) 18, 218, 320, 329, 357, 384, 426, 473
TfiI GAWTC 1 cut(s) 170
Tru1I TTAA 3 cut(s) 282, 458, 521
Tru9I TTAA 3 cut(s) 282, 458, 521
TscAI CASTG 5 cut(s) 51, 73, 158, 469, 616
TseFI GTSAC 1 cut(s) 604
TseI GCWGC 1 cut(s) 134
Tsp45I GTSAC 1 cut(s) 604
TspDTI ATGAA 7 cut(s) 50, 231, 329, 408, 420, 505, 622
TspRI CASTG 5 cut(s) 51, 73, 158, 469, 616
VpaK11BI GGWCC 1 cut(s) 310
XagI CCTNNNNNAGG 1 cut(s) 575
XapI RAATTY 2 cut(s) 329, 426
XceI RCATGY 1 cut(s) 529
XhoI CTCGAG 1 cut(s) 572
XmnI GAANNNNTTC 1 cut(s) 629
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.