Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr13

Orthogroup ID

OG0007581

Physical Location & Seq

Genomic Coordinates Forward (+)

1752726 .. 1756522

Gene Structure 3797 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD13G1024800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1752 bp

ATGGATGGCATTCGCCGCTGGTTTCAGGGCCTTTCTTCTTCTTCCGCCGATCACGGTGATGTTTCCTTAAACGACGCCGTCGAACGGCCACCGCCGTCATCGGCCAGCACTGATGAGGAGCAGAGGGAGTTGATGATGACGGTGGACCTGGACATGAGTGGATTGACGCCCATCAAGGTGCCGATGAGGACCAATCACAGAGTCGCCACTATGGACCATCATAAGAAGATGCTGGACAAGGAATTTTTCACAGAATATGGTGAGGCGAGCCAGTACGAAATCCAGGAAGTGATTGGGAAAGGTAGCTATGGAGTTGTTGCATCTGCAGTTGACACTCACACGGGTGAGAAAGTGGCCATTAAGAAGATCAACGACGTCTTCGAGCATGTCTCCGATGCTACGAGGATTTTGCGCGAAATTAAGCTCCTTCGGCTGCTGCATCACCCTGATATTGTCGAAATCAGGCATATAATGCTACCCCCTTGCCGGCGAGAATTTAAAGACATATACGTTGTGTTTGAGTTGATGGAATCTGACCTTCACCAAGTTATTAAGGCGAATGATGATCTCACTCCCGAGCATTATCAGTTCTTCTTGTACCAGCTTCTTCGCGCCCTCAAGTATATACATACAGCAAATGTCTTCCATCGAGATTTGAAGCCGAAAAATATACTTGCTAATGCAGACTGCAAACTGAAAATTTGTGACTTTGGGCTCGCTCGTGCTGCGTGTAATGACGCTCCATCAACCATTTTTTGGACTGATTATGTGGCAACTCGATGGTACCGTGCTCCTGAACTCTGTGGTTCCTTTTTCTCCAAGTACACCCCTGCTATTGATATATGGAGCATAGGGTGCATATTTGCAGAAATGCTAACAGGAAAACCGTTGTTTCCCGGGAAAAATGTTGTGCATCAATTGGATCTCATTACCGACTTACTTGGCACTCCTCCCACTGAATCTATTGCAAGGATTCGGAACGAAAAGGCCAGAAGATATTTAAGTAGCATGAGGAGAAAGAAACCAGTTCCTCTCTCGCAAAAAATCCCAAATGCGGACCCATTGGCTCTTCGTTTGCTTGAGCGTTTGCTTGCTTTTGATGCTAGAGATCGTCCTTCTGCTGAAGAGGCCTTAGCAGACCCATATTTTGTTGGATTGGCAAATTTGGACCAGGAACCGTCCAGACAGCCCATTTCAAAGCTCGAGTTTGAGTTCGAGAGGAGTAGATTGACAAAAGATGATGTGAGAGAGCTAATTTATAGAGAGATTTTGGAGTATCATCCGAAGATGCTGCAGGATTACCTTCAAGGTTCAGATAATATTGGCTTCATGTATCCAAGTGGAGTTGATCGATTTAGACGACAGTTTGAGCATCTTGAGGCACACTACGGCAAAGGTGAAAGAAGCACTGCGCTTCAAAGGAAGCATGCCTCGTTACCTAGGGAACGAGTGTGTATGTCGCAGGATGAGGCTGCGGAGCAAAATGGTAACGTTAAAAAGAGTACAACGGCTTCAGTGGGTCGTGCAGCTGTTCATAGCCCTCAAAGGTCTCAGGAGGTAAAAGTACCAGAACCAACATATCAAAATGGATCAACTATACCGAATGGCGTTAATAATCCAAACCATAGTCCCCACAGCCTGTGGAGGAGCGATAGCATTAGCGCTTCGAAGTGTGTGGTTATGAAAAGCCAGTACTGCGAGGAAGATCGAATTGCAGGGCGAAACGAAGAGATGGTCAACGTGCTGTCGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000165 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004707 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009987 GO:0010468 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019222 GO:0019538 GO:0023014 GO:0023052 GO:0035556 GO:0036211 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0060255 GO:0065007 GO:0071704 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko04016 map04016

Pfam Domains

Protein Families

Pkinase

Protein Analysis

584

Amino Acids

66.53

Weight (kDa)

6.61

Isoelectric Point (pI)

44.5

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	92 - 383	6.4e-66	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	93 - 303	2.2e-31	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007581)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G01450 AT2G01450 AT2G01450 AT2G01450
fragaria_vesca	FvH4_4g32501 FvH4_4g32501 FvH4_4g32501 FvH4_4g32501 FvH4_4g32501
malus_domestica	MD13G1024800.v1.1
prunus_persica	Prupe.1G325700_v2.0.a1 Prupe.1G325700_v2.0.a1 Prupe.1G325700_v2.0.a1 Prupe.1G325700_v2.0.a1 Prupe.1G325700_v2.0.a1 Prupe.1G325700_v2.0.a1
rosa_chinensis	RchiOBHm_Chr4g0441371
rosa_laevigata	RLG00000006097
rosa_multiflora	Rmu_sc0005516.1_g000005
rosa_rugosa	Rorug04G0332800
rosa_samantha	Rh4AG384800 Rh4BG398800 Rh4CG413900
rosa_wichuraiana	Rw4G033220

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	378
Acc65I	GGTACC	1 cut(s)	783
AccB1I	GGYRCC	2 cut(s)	178, 783
AccB7I	CCANNNNNTGG	1 cut(s)	756
AccII	CGCG	2 cut(s)	414, 612
AciI	CCGC	5 cut(s)	16, 45, 92, 1055, 1475
AclI	AACGTT	1 cut(s)	1491
AclWI	GGATC	2 cut(s)	930, 1597
AcoI	YGGCCR	3 cut(s)	86, 102, 354
AcsI	RAATTY	4 cut(s)	242, 494, 699, 1162
AcuI	CTGAAG	2 cut(s)	1143, 1497
AcyI	GRCGYC	3 cut(s)	75, 167, 375
AfaI	GTAC	7 cut(s)	275, 599, 785, 824, 1504, 1566, 1694
AfeI	AGCGCT	1 cut(s)	1663
AfiI	CCNNNNNNNGG	4 cut(s)	84, 486, 756, 1054
AgsI	TTSAA	4 cut(s)	658, 1197, 1307, 1418
AjnI	CCWGG	3 cut(s)	147, 282, 1170
AleI	CACNNNNGTG	1 cut(s)	342
AluBI	AGCT	6 cut(s)	306, 424, 604, 1201, 1252, 1529
AluI	AGCT	6 cut(s)	306, 424, 604, 1201, 1252, 1529
Alw21I	GWGCWC	1 cut(s)	793
Alw26I	GTCTC	2 cut(s)	394, 1554
AlwI	GGATC	2 cut(s)	930, 1597
Ama87I	CYCGRG	3 cut(s)	575, 896, 1202
Aor51HI	AGCGCT	1 cut(s)	1663
AoxI	GGCC	6 cut(s)	28, 86, 102, 354, 987, 1128
ApeKI	GCWGC	6 cut(s)	433, 436, 725, 1291, 1472, 1526
ApoI	RAATTY	4 cut(s)	242, 494, 699, 1162
ArsI	GACNNNNNNTTYG	4 cut(s)	1130, 1162, 1719, 1751
Asp718I	GGTACC	1 cut(s)	783
AspA2I	CCTAGG	1 cut(s)	1439
AspLEI	GCGC	4 cut(s)	414, 614, 1414, 1664
AspS9I	GGNCC	6 cut(s)	28, 145, 189, 214, 1057, 1168
AsuC2I	CCSGG	2 cut(s)	897, 898
AsuHPI	GGTGA	6 cut(s)	68, 272, 356, 434, 533, 1409
AsuII	TTCGAA	1 cut(s)	1667
AvaI	CYCGRG	3 cut(s)	575, 896, 1202
AvaII	GGWCC	5 cut(s)	145, 189, 214, 1057, 1168
AvrII	CCTAGG	1 cut(s)	1439
BalI	TGGCCA	1 cut(s)	356
BanI	GGYRCC	2 cut(s)	178, 783
BanII	GRGCYC	1 cut(s)	717
BarI	GAAGNNNNNNTAC	2 cut(s)	1495, 1527
BauI	CACGAG	1 cut(s)	720
BbsI	GAAGAC	2 cut(s)	370, 634
Bbv12I	GWGCWC	1 cut(s)	793
BbvI	GCAGC	6 cut(s)	420, 423, 712, 1278, 1459, 1538
BccI	CCATC	7 cut(s)	179, 225, 520, 654, 751, 774, 1726
BceAI	ACGGC	5 cut(s)	62, 79, 101, 1405, 1524
BcgI	CGANNNNNNTGC	4 cut(s)	391, 425, 1273, 1307
BciT130I	CCWGG	3 cut(s)	149, 284, 1172
BciVI	GTATCC	1 cut(s)	1344
BcnI	CCSGG	2 cut(s)	897, 898
BcoDI	GTCTC	2 cut(s)	394, 1554
BfaI	CTAG	2 cut(s)	1104, 1440
BfmI	CTRYAG	2 cut(s)	324, 1292
BfoI	RGCGCY	1 cut(s)	1665
BfuI	GTATCC	1 cut(s)	1344
BisI	GCNGC	7 cut(s)	16, 434, 437, 726, 1292, 1473, 1527
BlnI	CCTAGG	1 cut(s)	1439
BlsI	GCNGC	7 cut(s)	17, 435, 438, 727, 1293, 1474, 1528
BmcAI	AGTACT	1 cut(s)	1694
Bme1390I	CCNGG	5 cut(s)	149, 284, 897, 898, 1172
Bme18I	GGWCC	5 cut(s)	145, 189, 214, 1057, 1168
BmeT110I	CYCGRG	3 cut(s)	575, 896, 1202
BmgT120I	GGNCC	6 cut(s)	28, 145, 189, 214, 1057, 1168
BmiI	GGNNCC	5 cut(s)	180, 785, 808, 1059, 1176
BmrFI	CCNGG	5 cut(s)	149, 284, 897, 898, 1172
BmsI	GCATC	8 cut(s)	219, 329, 385, 448, 922, 1090, 1278, 1381
BpiI	GAAGAC	2 cut(s)	370, 634
BplI	GAGNNNNNCTC	2 cut(s)	374, 406
Bpu10I	CCTNAGC	1 cut(s)	1132
Bpu14I	TTCGAA	1 cut(s)	1667
BpuEI	CTTGAG	3 cut(s)	602, 1100, 1397
BpuMI	CCSGG	2 cut(s)	897, 898
Bsa29I	ATCGAT	1 cut(s)	1351
BsaHI	GRCGYC	3 cut(s)	75, 167, 375
BsaI	GGTCTC	1 cut(s)	1554
BsaJI	CCNNGG	2 cut(s)	896, 1439
Bsc4I	CCNNNNNNNGG	4 cut(s)	84, 486, 756, 1054
Bse118I	RCCGGY	1 cut(s)	486
Bse1I	ACTGG	3 cut(s)	271, 1025, 1690
BseBI	CCWGG	3 cut(s)	149, 284, 1172
BseCI	ATCGAT	1 cut(s)	1351
BseDI	CCNNGG	2 cut(s)	896, 1439
BseGI	GGATG	3 cut(s)	10, 1279, 1471
BseLI	CCNNNNNNNGG	4 cut(s)	84, 486, 756, 1054
BseMII	CTCAG	1 cut(s)	1565
BseNI	ACTGG	3 cut(s)	271, 1025, 1690
BseRI	GAGGAG	5 cut(s)	131, 939, 1027, 1234, 1660
BseXI	GCAGC	6 cut(s)	420, 423, 712, 1278, 1459, 1538
BsgI	GTGCAG	1 cut(s)	1545
Bsh1236I	CGCG	2 cut(s)	414, 612
BshFI	GGCC	6 cut(s)	30, 88, 104, 356, 989, 1130
BshNI	GGYRCC	2 cut(s)	178, 783
BshVI	ATCGAT	1 cut(s)	1351
BsiHKAI	GWGCWC	1 cut(s)	793
BsiHKCI	CYCGRG	3 cut(s)	575, 896, 1202
BsiSI	CCGG	2 cut(s)	487, 897
BslFI	GGGAC	1 cut(s)	1614
BslI	CCNNNNNNNGG	4 cut(s)	84, 486, 756, 1054
BsmAI	GTCTC	2 cut(s)	394, 1554
BsmFI	GGGAC	1 cut(s)	1614
BsmI	GAATGC	1 cut(s)	9
BsnI	GGCC	6 cut(s)	30, 88, 104, 356, 989, 1130
Bso31I	GGTCTC	1 cut(s)	1554
BsoBI	CYCGRG	3 cut(s)	575, 896, 1202
Bsp119I	TTCGAA	1 cut(s)	1667
Bsp1286I	GDGCHC	2 cut(s)	717, 793
Bsp143I	GATC	8 cut(s)	49, 366, 565, 922, 1108, 1348, 1589, 1705
BspACI	CCGC	5 cut(s)	16, 45, 92, 1055, 1475
BspANI	GGCC	6 cut(s)	30, 88, 104, 356, 989, 1130
BspCNI	CTCAG	1 cut(s)	1564
BspDI	ATCGAT	1 cut(s)	1351
BspFNI	CGCG	2 cut(s)	414, 612
BspLI	GGNNCC	5 cut(s)	180, 785, 808, 1059, 1176
BspMAI	CTGCAG	2 cut(s)	328, 1296
BspPI	GGATC	2 cut(s)	930, 1597
BspQI	GCTCTTC	1 cut(s)	1074
BspT104I	TTCGAA	1 cut(s)	1667
BspT107I	GGYRCC	2 cut(s)	178, 783
BspTNI	GGTCTC	1 cut(s)	1554
BsrFI	RCCGGY	1 cut(s)	486
BsrI	ACTGG	3 cut(s)	271, 1025, 1690
BssAI	RCCGGY	1 cut(s)	486
BssECI	CCNNGG	2 cut(s)	896, 1439
BssMI	GATC	8 cut(s)	49, 366, 565, 922, 1108, 1348, 1589, 1705
BssNI	GRCGYC	3 cut(s)	75, 167, 375
BssSI	CACGAG	1 cut(s)	720
BssT1I	CCWWGG	1 cut(s)	1439
Bst2BI	CACGAG	1 cut(s)	720
Bst2UI	CCWGG	3 cut(s)	149, 284, 1172
Bst4CI	ACNGT	6 cut(s)	56, 142, 788, 888, 1179, 1365
Bst6I	CTCTTC	3 cut(s)	1074, 1119, 1722
BstACI	GRCGYC	3 cut(s)	75, 167, 375
BstAPI	GCANNNNNTGC	2 cut(s)	472, 855
BstBI	TTCGAA	1 cut(s)	1667
BstC8I	GCNNGC	6 cut(s)	106, 268, 488, 717, 1092, 1428
BstDEI	CTNAG	2 cut(s)	1132, 1551
BstF5I	GGATG	3 cut(s)	10, 1279, 1471
BstFNI	CGCG	2 cut(s)	414, 612
BstH2I	RGCGCY	1 cut(s)	1665
BstHHI	GCGC	4 cut(s)	414, 614, 1414, 1664
BstKTI	GATC	8 cut(s)	52, 369, 568, 925, 1111, 1351, 1592, 1708
BstMAI	GTCTC	2 cut(s)	394, 1554
BstMBI	GATC	8 cut(s)	49, 366, 565, 922, 1108, 1348, 1589, 1705
BstMWI	GCNNNNNNNGC	8 cut(s)	15, 430, 472, 725, 855, 1100, 1127, 1695
BstNI	CCWGG	3 cut(s)	149, 284, 1172
BstNSI	RCATGY	2 cut(s)	389, 1430
BstSCI	CCNGG	5 cut(s)	147, 282, 895, 896, 1170
BstSFI	CTRYAG	2 cut(s)	324, 1292
BstUI	CGCG	2 cut(s)	414, 612
BstV1I	GCAGC	6 cut(s)	420, 423, 712, 1278, 1459, 1538
BstV2I	GAAGAC	2 cut(s)	370, 634
BstX2I	RGATCY	1 cut(s)	922
BstYI	RGATCY	1 cut(s)	922
Bsu15I	ATCGAT	1 cut(s)	1351
BsuI	GTATCC	1 cut(s)	1344
BsuRI	GGCC	6 cut(s)	30, 88, 104, 356, 989, 1130
BsuTUI	ATCGAT	1 cut(s)	1351
BtsCI	GGATG	3 cut(s)	10, 1279, 1471
BtsI	GCAGTG	1 cut(s)	1407
BtsIMutI	CAGTG	4 cut(s)	108, 954, 1407, 1521
Cac8I	GCNNGC	6 cut(s)	106, 268, 488, 717, 1092, 1428
CfoI	GCGC	4 cut(s)	414, 614, 1414, 1664
Cfr10I	RCCGGY	1 cut(s)	486
Cfr13I	GGNCC	6 cut(s)	28, 145, 189, 214, 1057, 1168
Cfr9I	CCCGGG	1 cut(s)	896
ClaI	ATCGAT	1 cut(s)	1351
CseI	GACGC	3 cut(s)	83, 175, 746
Csp6I	GTAC	7 cut(s)	274, 598, 784, 823, 1503, 1565, 1693
CviAII	CATG	5 cut(s)	154, 386, 1009, 1330, 1427
CviQI	GTAC	7 cut(s)	274, 598, 784, 823, 1503, 1565, 1693
DdeI	CTNAG	2 cut(s)	1132, 1551
DpnI	GATC	8 cut(s)	51, 368, 567, 924, 1110, 1350, 1591, 1707
DpnII	GATC	8 cut(s)	49, 366, 565, 922, 1108, 1348, 1589, 1705
DraI	TTTAAA	1 cut(s)	499
EaeI	YGGCCR	3 cut(s)	86, 102, 354
Eam1104I	CTCTTC	3 cut(s)	1074, 1119, 1722
EarI	CTCTTC	3 cut(s)	1074, 1119, 1722
EciI	GGCGGA	1 cut(s)	34
Eco130I	CCWWGG	1 cut(s)	1439
Eco147I	AGGCCT	1 cut(s)	1130
Eco24I	GRGCYC	1 cut(s)	717
Eco31I	GGTCTC	1 cut(s)	1554
Eco47I	GGWCC	5 cut(s)	145, 189, 214, 1057, 1168
Eco47III	AGCGCT	1 cut(s)	1663
Eco57I	CTGAAG	2 cut(s)	1143, 1497
Eco88I	CYCGRG	3 cut(s)	575, 896, 1202
EcoO109I	RGGNCCY	1 cut(s)	28
EcoRII	CCWGG	3 cut(s)	147, 282, 1170
EcoT14I	CCWWGG	1 cut(s)	1439
EcoT38I	GRGCYC	1 cut(s)	717
ErhI	CCWWGG	1 cut(s)	1439
FaeI	CATG	5 cut(s)	157, 389, 1012, 1333, 1430
FaqI	GGGAC	1 cut(s)	1614
FatI	CATG	5 cut(s)	153, 385, 1008, 1329, 1426
Fnu4HI	GCNGC	7 cut(s)	16, 434, 437, 726, 1292, 1473, 1527
FokI	GGATG	3 cut(s)	17, 1266, 1478
FriOI	GRGCYC	1 cut(s)	717
Fsp4HI	GCNGC	7 cut(s)	16, 434, 437, 726, 1292, 1473, 1527
FspBI	CTAG	2 cut(s)	1104, 1440
GlaI	GCGC	4 cut(s)	413, 613, 1413, 1663
GluI	GCNGC	7 cut(s)	16, 434, 437, 726, 1292, 1473, 1527
HaeII	RGCGCY	1 cut(s)	1665
HaeIII	GGCC	6 cut(s)	30, 88, 104, 356, 989, 1130
HapII	CCGG	2 cut(s)	487, 897
HgaI	GACGC	3 cut(s)	83, 175, 746
HhaI	GCGC	4 cut(s)	414, 614, 1414, 1664
Hin1I	GRCGYC	3 cut(s)	75, 167, 375
Hin1II	CATG	5 cut(s)	157, 389, 1012, 1333, 1430
Hin6I	GCGC	4 cut(s)	412, 612, 1412, 1662
HinP1I	GCGC	4 cut(s)	412, 612, 1412, 1662
HincII	GTYRAC	2 cut(s)	331, 1738
HindII	GTYRAC	2 cut(s)	331, 1738
HinfI	GANTC	4 cut(s)	201, 530, 959, 973
HpaII	CCGG	2 cut(s)	487, 897
HphI	GGTGA	6 cut(s)	68, 272, 356, 434, 533, 1409
Hpy166II	GTNNAC	4 cut(s)	145, 331, 825, 1738
Hpy188I	TCNGA	5 cut(s)	394, 535, 978, 1284, 1315
Hpy188III	TCNNGA	7 cut(s)	575, 650, 794, 1182, 1216, 1376, 1553
Hpy8I	GTNNAC	4 cut(s)	145, 331, 825, 1738
Hpy99I	CGWCG	3 cut(s)	77, 83, 377
HpyAV	CCTTC	4 cut(s)	437, 548, 1125, 1313
HpyCH4III	ACNGT	6 cut(s)	56, 142, 788, 888, 1179, 1365
HpyCH4IV	ACGT	4 cut(s)	375, 510, 1491, 1740
HpyF10VI	GCNNNNNNNGC	8 cut(s)	15, 430, 472, 725, 855, 1100, 1127, 1695
HpyF3I	CTNAG	2 cut(s)	1132, 1551
HpySE526I	ACGT	4 cut(s)	375, 510, 1491, 1740
Hsp92I	GRCGYC	3 cut(s)	75, 167, 375
Hsp92II	CATG	5 cut(s)	157, 389, 1012, 1333, 1430
HspAI	GCGC	4 cut(s)	412, 612, 1412, 1662
KpnI	GGTACC	1 cut(s)	787
KroI	GCCGGC	1 cut(s)	486
KroNI	GCCGGC	1 cut(s)	488
Kzo9I	GATC	8 cut(s)	49, 366, 565, 922, 1108, 1348, 1589, 1705
LguI	GCTCTTC	1 cut(s)	1074
LmnI	GCTCC	7 cut(s)	118, 429, 745, 796, 846, 1477, 1647
Lsp1109I	GCAGC	6 cut(s)	420, 423, 712, 1278, 1459, 1538
LweI	GCATC	8 cut(s)	219, 329, 385, 448, 922, 1090, 1278, 1381
MaeI	CTAG	2 cut(s)	1104, 1440
MaeII	ACGT	4 cut(s)	375, 510, 1491, 1740
MaeIII	GTNAC	3 cut(s)	704, 1434, 1487
MalI	GATC	8 cut(s)	51, 368, 567, 924, 1110, 1350, 1591, 1707
MboI	GATC	8 cut(s)	49, 366, 565, 922, 1108, 1348, 1589, 1705
MfeI	CAATTG	1 cut(s)	917
MflI	RGATCY	1 cut(s)	922
MhlI	GDGCHC	2 cut(s)	717, 793
MlsI	TGGCCA	1 cut(s)	356
MluCI	AATT	8 cut(s)	242, 417, 494, 699, 917, 1162, 1254, 1710
MluNI	TGGCCA	1 cut(s)	356
MlyI	GAGTC	1 cut(s)	210
MmeI	TCCRAC	1 cut(s)	1132
Mox20I	TGGCCA	1 cut(s)	356
MroNI	GCCGGC	1 cut(s)	486
MscI	TGGCCA	1 cut(s)	356
MseI	TTAA	8 cut(s)	68, 360, 420, 498, 552, 1001, 1494, 1611
MslI	CAYNNNNRTG	3 cut(s)	57, 176, 342
Msp20I	TGGCCA	1 cut(s)	356
MspA1I	CMGCKG	2 cut(s)	18, 1529
MspI	CCGG	2 cut(s)	487, 897
MspR9I	CCNGG	5 cut(s)	149, 284, 897, 898, 1172
MunI	CAATTG	1 cut(s)	917
Mva1269I	GAATGC	1 cut(s)	9
MvaI	CCWGG	3 cut(s)	149, 284, 1172
MvnI	CGCG	2 cut(s)	414, 612
MwoI	GCNNNNNNNGC	8 cut(s)	15, 430, 472, 725, 855, 1100, 1127, 1695
NaeI	GCCGGC	1 cut(s)	488
NciI	CCSGG	2 cut(s)	897, 898
NdeII	GATC	8 cut(s)	49, 366, 565, 922, 1108, 1348, 1589, 1705
NgoMIV	GCCGGC	1 cut(s)	486
NlaIII	CATG	5 cut(s)	157, 389, 1012, 1333, 1430
NlaIV	GGNNCC	5 cut(s)	180, 785, 808, 1059, 1176
NmuCI	GTSAC	1 cut(s)	704
NspI	RCATGY	2 cut(s)	389, 1430
NspV	TTCGAA	1 cut(s)	1667
OliI	CACNNNNGTG	1 cut(s)	342
PaeI	GCATGC	1 cut(s)	1430
PaeR7I	CTCGAG	1 cut(s)	1202
PceI	AGGCCT	1 cut(s)	1130
PciSI	GCTCTTC	1 cut(s)	1074
PcsI	WCGNNNNNNNCGW	4 cut(s)	78, 378, 784, 1357
PctI	GAATGC	1 cut(s)	9
PdiI	GCCGGC	1 cut(s)	488
PfeI	GAWTC	3 cut(s)	530, 959, 973
PflFI	GACNNNGTC	1 cut(s)	77
PflMI	CCANNNNNTGG	1 cut(s)	756
PfoI	TCCNGGA	1 cut(s)	282
PkrI	GCNGC	7 cut(s)	17, 435, 438, 727, 1293, 1474, 1528
PleI	GAGTC	1 cut(s)	209
PpsI	GAGTC	1 cut(s)	209
Psp1406I	AACGTT	1 cut(s)	1491
Psp6I	CCWGG	3 cut(s)	147, 282, 1170
PspGI	CCWGG	3 cut(s)	147, 282, 1170
PspN4I	GGNNCC	5 cut(s)	180, 785, 808, 1059, 1176
PspPI	GGNCC	6 cut(s)	28, 145, 189, 214, 1057, 1168
PspXI	VCTCGAGB	1 cut(s)	1202
PstI	CTGCAG	2 cut(s)	328, 1296
PsuI	RGATCY	1 cut(s)	922
PsyI	GACNNNGTC	1 cut(s)	77
PvuII	CAGCTG	1 cut(s)	1529
RsaI	GTAC	7 cut(s)	275, 599, 785, 824, 1504, 1566, 1694
RsaNI	GTAC	7 cut(s)	274, 598, 784, 823, 1503, 1565, 1693
RseI	CAYNNNNRTG	3 cut(s)	57, 176, 342
SapI	GCTCTTC	1 cut(s)	1074
SaqAI	TTAA	8 cut(s)	68, 360, 420, 498, 552, 1001, 1494, 1611
SatI	GCNGC	7 cut(s)	16, 434, 437, 726, 1292, 1473, 1527
Sau3AI	GATC	8 cut(s)	49, 366, 565, 922, 1108, 1348, 1589, 1705
Sau96I	GGNCC	6 cut(s)	28, 145, 189, 214, 1057, 1168
ScaI	AGTACT	1 cut(s)	1694
SchI	GAGTC	1 cut(s)	210
ScrFI	CCNGG	5 cut(s)	149, 284, 897, 898, 1172
SduI	GDGCHC	2 cut(s)	717, 793
SfaNI	GCATC	8 cut(s)	219, 329, 385, 448, 922, 1090, 1278, 1381
SfcI	CTRYAG	2 cut(s)	324, 1292
Sfr274I	CTCGAG	1 cut(s)	1202
SfuI	TTCGAA	1 cut(s)	1667
SinI	GGWCC	5 cut(s)	145, 189, 214, 1057, 1168
SlaI	CTCGAG	1 cut(s)	1202
SmaI	CCCGGG	1 cut(s)	898
SmiMI	CAYNNNNRTG	3 cut(s)	57, 176, 342
SmlI	CTYRAG	4 cut(s)	617, 1079, 1202, 1376
SmoI	CTYRAG	4 cut(s)	617, 1079, 1202, 1376
SphI	GCATGC	1 cut(s)	1430
Sse9I	AATT	8 cut(s)	242, 417, 494, 699, 917, 1162, 1254, 1710
SseBI	AGGCCT	1 cut(s)	1130
SsiI	CCGC	5 cut(s)	16, 45, 92, 1055, 1475
SspI	AATATT	1 cut(s)	1321
SspMI	CTAG	2 cut(s)	1104, 1440
StuI	AGGCCT	1 cut(s)	1130
StyD4I	CCNGG	5 cut(s)	147, 282, 895, 896, 1170
StyI	CCWWGG	1 cut(s)	1439
TaaI	ACNGT	6 cut(s)	56, 142, 788, 888, 1179, 1365
TaiI	ACGT	4 cut(s)	378, 513, 1494, 1743
TasI	AATT	8 cut(s)	242, 417, 494, 699, 917, 1162, 1254, 1710
TatI	WGTACW	3 cut(s)	822, 1502, 1692
TauI	GCSGC	1 cut(s)	18
TfiI	GAWTC	3 cut(s)	530, 959, 973
Tru1I	TTAA	8 cut(s)	68, 360, 420, 498, 552, 1001, 1494, 1611
Tru9I	TTAA	8 cut(s)	68, 360, 420, 498, 552, 1001, 1494, 1611
TscAI	CASTG	4 cut(s)	115, 961, 1414, 1521
TseFI	GTSAC	1 cut(s)	704
TseI	GCWGC	6 cut(s)	433, 436, 725, 1291, 1472, 1526
Tsp45I	GTSAC	1 cut(s)	704
TspDTI	ATGAA	3 cut(s)	1318, 1523, 1697
TspMI	CCCGGG	1 cut(s)	896
TspRI	CASTG	4 cut(s)	115, 961, 1414, 1521
Tth111I	GACNNNGTC	1 cut(s)	77
Van91I	CCANNNNNTGG	1 cut(s)	756
VpaK11BI	GGWCC	5 cut(s)	145, 189, 214, 1057, 1168
XapI	RAATTY	4 cut(s)	242, 494, 699, 1162
XceI	RCATGY	2 cut(s)	389, 1430
XcmI	CCANNNNNNNNNTGG	1 cut(s)	290
XhoI	CTCGAG	1 cut(s)	1202
XmaI	CCCGGG	1 cut(s)	896
XmaJI	CCTAGG	1 cut(s)	1439
XspI	CTAG	2 cut(s)	1104, 1440
ZraI	GACGTC	1 cut(s)	376
ZrmI	AGTACT	1 cut(s)	1694

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.