Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000004

Orthogroup ID

OG0007581

Physical Location & Seq

Genomic Coordinates Forward (+)

50695943 .. 50704566

Gene Structure 8624 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug04G0332800.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4212 bp

ATGGACAGCAGAGGGGCACGTACATGTTCATACACGTACATGCCACTCATGGTTCGCTTGATACGAATTCGGCTGAATCACAATACACAACTTCTTCGGCACAGAGTGATTTCAAAGGTTTTGGAGTTGGGAATTGTAGTGCACTCAGTTAATATTGGAATTTCATTGGGTGCTTCTGAAAGTGCTGAAACAATTCGACCTCTTCTAGTTGCTTTGACTTTCCATCAAGTTTTTGAAGGCATGGGCCATGGGACTTGGTGCTTGCATAGCTCAGAGGAAAAGGAAGATAAAACTCTCGTCAAAGTTGAAAGGATCGCCAATCCCGAAGACTTTGCCAAAGCTTTGATCCTCCAAAGGTACTTCAACTACTGCAATTTGTTGATCACTAACAAGATGGACAGTACTGACAGTCACACTAATGCTGGAATATGGAGCAAAATGACATTCCAGTGGATGAATCCAATTTTTAAAAAGGGACTTGAGATCCAAAAACTGGAACTATCTCATATACCATCTATTCCTCCTTCAGTGAGAGCTGAGAGTGCTTCTTCTTTGCTCCAAGAATCACTCGAACACCACTCTTCATTATTAAAAGCCATAGGGAGGGCTGTATGGAAATCACTAGCCATAAATGCAGTTTTTGCAGGAATCAATGTTGTTGCATCCTATACTGGTCCCTTCTTAATCAGATACTTTGTGAACCATATAGTTGGAAAGTATGGAAGCACAAACATGTATTATGGAGAGAAGCTTGCCTTCATATTCTTTTTTGCAATGACTTTGGATTCAATTACTCAAAGGCAACTGTCTTTTCGTTCTCGGCAAATTGGTCTACAAGTAAGAGCAGCTCTGACCTCCTTAATCTACAAGAAGTGCATCTTGATTAAGTACTTTGGTCCAAGCAATGGTAAAATCGTAAACTTAATCAACATGGATGTAGAAGTGGTTGGAGAATTCTTCTCTTCTATTCATGGAGTTTGGGTGCTTCTCCTTCAAGTTGTTCTGGCATTGGTCATCTTGTACATAAATCTCGGTGCTGCCCCTTCTCTGGCAGCCTTTCTGACCACCGTAGTAGTAGTGGTATGCAACACACCATTGGCTCGAAGGCAACAAAATCTCCAATCAAAAATCATGGAAGCAAAGGATTCAAGAATTAAGAGAACTGCAGAGACTCTAAAGAATATGAGAGTCGTAAAATTTAATTCATGGGAAACTAGTTTCTTAGAGAAGCTCCTTCAACTCAGAGAAACTGAGAAAAGTTGGTTAAAGAGATTTTTGTACACCAAGGCGGCAGTGGTATTTCTTTTCTGGACTTCACCAACTATAGTTACAGGGATTACTTTTGGTGTTTGCATCATGATGAAAACGAAATTAGAACCAAGTACAATCCTGTCAGCTTTAGCCACTTTCAGAATCCTTACTGAGCCTATTTACAACCTTCCAGAGTTTATCTCGATGATTATTCAAACAAAGGTTTCGGTTAAGCGAATTCAAGAATTCATCAGATTAGAAGAACAAGTGGAGCTGATTCCTCGTGATTTTTCTTGCACCACATCATCCACTTCAAAAATCAGGGTTGAAATGGAGTCCAGTGAGTTTTCTTGGAAACCAAGCAATGAACATATAGAGAGACCTACAATCACAGTTAGAGAAAAACTAACTATGAGAAAGGGTTGCAAGGTGGGAGTCTGCGGTTCTGTTGGCTCAGGCAAGTCTAGCCTACTATGCAGTATACTTCGTGAGATTCCACAAATTTCTGGGCAAGCAACCAAGTGTTATGGAACAAAATCTTATGTTGCACAAAGTCCATGGATCCAAACTGGAACCATAAGGGACAACATCTTGTTTGGTAAGGAGATGAATGAGGGTTTTTATAAGAACATTGTGGAATGTTGTGCTTTGAACCAGGATTTCAAGATGTGGCCTAACGGAGATTTGACAGTCGTGGGGGAAAGAGGAATAAATCTAAGTGGAGGCCAAAAACAAAGGATTCAACTCGCAAGAGCTGTCTACAGTGATGCTGATGTATATATCTTAGACGACCCTTTCAGTGCTGTAGATGCACATACAGGAACACATTTGTTCACGAAATGTATCTTGCAAGAATTGTCTGAAAAAACCGTAATCTATGCTACTCACCAACTAGAATTTTTGGAAGCAGCAGACTATGTTTTGGTAGTTAAAGATGGTCAAATTGCTCAATCTGGCACTTATGGGAAGTTGATTGAAGATCCTAATGGTGAACTTGTCATACAAATGACTGCACATAGAAAATCTCTACACCAAGTTCACAGCAGTCAACGACCGGATTCTTTAAGCAACACCACAACTACTGGCCCCATGAAAAATGGAATTGCTGAAAGAGTATTGGATATTGAAGAAAAGGAAAAAGCTTCAACCAATGACCGGATTACTGTCAAAGAGATCCGAGAAGAAGAAGAAGAAGCACACACTGGTCGCGTAAAATGGAGTGTATATTCTACATTTGTGACTTGTGCTTTTAGAGGTCTACTGCTTCCAGTAATCCTTGTCTGTCACGTTCTCTTCCAAGTGCTCCAGATGGCAACCAACTACTGGATTGCCTGGGCAGTAGGGAAGGAAAACACTGTCACCAGAGGAAATATGATATGGATTTTCGCTCTTTTATCTGGTGCAAGCTCTGTCTTCCTCTTGGGAAGGGCTGTTTTCCTTTCAGTTATCACTATTGAAACTTCCCAGCATCTCTTTGTTGGGATGATTACTTCAGTTTTCCGAGCGCCTATGGCATTTTTCGACTCTACACCTTCTAGTCGAATCCTCACTAGGTCTTCCACAGATCAAAGCACAGTTGACACAGATATCACATTCAGGTTGGGGGGGCTGGTATTTGCACTCATTCAACTAGTATGCACTATAATCCTTATGTGTCTCGTGGCCTGGCAATTGTCCCTACTTTTCCTTGTCGTCGTTGCAATTTCTTTTTGGTACATGAAGTACTATATTACCACTGCTAGAGAACTGGCCAGAATGGTTGCAACTCAGAAGGCTCCAATACTGCATCATTTTTCAGAATCAATTGTAGGAGCTACAACAATTCGTTGTTTCAATCAACAAAATCGATTCTTGACAAAAGCAATGAATGAAATTGATGACTTTTCTCGAGTAGCCTTTCATAACAATGCAACAATGGAATGGCTATCTGTGAGAATAAATTTTCTGTTCAATCTTGCCTTCCTTGTAGTTCTTATTGTCTTGGTGAACCAGCCTAAGTCTGCCATTGACCCTACTTTGGCAGGAGTGGCTGCATCATATGGTCTAAACGTTACTGTCCTGCAGGCTTGGGTGATTTGGAATCTATGCAATGTTGAAAACAAAATGATATCTGTTGAGAGAATTCTTCAATTCACTGGAATAGAAAGTGAGGCACCACTTGTGATCGAAGCTTCTAGGCAAAACTCCGAATGGCCAACTGAAGGACAAATTGAATTTCAAAACGTGCAAGTTCAATATCAGCCTGAGGGTCCAATGGTACTGAAAGGGATCACTTGCACATTCTCTAAAAAAGAGAAAATTGGAGTGGTGGGAAGAACAGGAAGTGGAAAGTCCACTATGATCCAAGCCTTGTTTAGAGTTGTTGAGCTCTCAGGAGGGAAAATTCTTATTGATGAACAAGACATTTCTAATATAGGCCTGCAGGATTTAAGGTCAAGTTTAGGCATAATCCCGCAAGACCCAACTTTATTTCAAGGAAGCTTGAGGAATAATCTGGATCCTCTGCAGCAACATTCTGATCCAGAAATATTGGAGGTTGTGAAGAAATGTCGACTTGGAAACTTAGTGAGGCAAGACCAAATGCTCCTGGATGCACCTGTTTCTGAAGATGGAGAAAACTGGAGCGTCGGACAGAGGCAGCTTATTTCTCTAGCCAGAGTGCTTCTGAAGAAGAGGAAAATTCTAGTGCTAGATGAGGCTACTGCATCTATAGATACAGGAACAGATAATGCAATTCAGGAGACAATAAGAAAAGAGACAAGTGAATGCACAGTTATCACCGTGGCTCACCGGATTCCTACTGTTATGGACAGTGACATGGTTCTAGTTCTTGATGAAGGTAGGATTGTGGAGTATGATTCTCCTGCTCGGCTAATGAAGGATAACTCTTCCGCATTTTCAAAGTTGGTTGCGGAATTCTTGAGGAGATCTTCCAGGAGAAAGAACAACGGCTCATCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000165 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004707 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009987 GO:0010468 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019222 GO:0019538 GO:0023014 GO:0023052 GO:0035556 GO:0036211 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0060255 GO:0065007 GO:0071704 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko04016 map04016

Pfam Domains

Protein Families

Pkinase

Protein Analysis

1403

Amino Acids

157.49

Weight (kDa)

8.8

Isoelectric Point (pI)

38.47

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Zip	PF02535	27 - 85	2.9e-13	ZIP Zinc transporter
ABC_membrane	PF00664	210 - 476	1.3e-19	ABC transporter transmembrane region
ABC_tran	PF00005	552 - 683	1.6e-14	ABC transporter
ABC_membrane	PF00664	843 - 1077	2.1e-32	ABC transporter transmembrane region
ABC_tran	PF00005	1171 - 1319	7.7e-26	ABC transporter

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0007581)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G01450 AT2G01450 AT2G01450 AT2G01450
fragaria_vesca	FvH4_4g32501 FvH4_4g32501 FvH4_4g32501 FvH4_4g32501 FvH4_4g32501
malus_domestica	MD13G1024800.v1.1
prunus_persica	Prupe.1G325700_v2.0.a1 Prupe.1G325700_v2.0.a1 Prupe.1G325700_v2.0.a1 Prupe.1G325700_v2.0.a1 Prupe.1G325700_v2.0.a1 Prupe.1G325700_v2.0.a1
rosa_chinensis	RchiOBHm_Chr4g0441371
rosa_laevigata	RLG00000006097
rosa_multiflora	Rmu_sc0005516.1_g000005
rosa_rugosa	Rorug04G0332800
rosa_samantha	Rh4AG384800 Rh4BG398800 Rh4CG413900
rosa_wichuraiana	Rw4G033220

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1875
AccB1I	GGYRCC	1 cut(s)	3403
AccB7I	CCANNNNNTGG	3 cut(s)	493, 905, 2574
AccI	GTMKAC	5 cut(s)	832, 1732, 2010, 2509, 3802
AccII	CGCG	1 cut(s)	2460
AciI	CCGC	5 cut(s)	1289, 1692, 3704, 4143, 4163
AclI	AACGTT	1 cut(s)	3300
AcoI	YGGCCR	2 cut(s)	3000, 3443
AcuI	CTGAAG	5 cut(s)	510, 2727, 3471, 3876, 3938
AdeI	CACNNNGTG	1 cut(s)	106
AfiI	CCNNNNNNNGG	8 cut(s)	493, 603, 905, 1048, 2406, 2574, 3268, 3452
AflIII	ACRYGT	3 cut(s)	23, 33, 732
AhlI	ACTAGT	2 cut(s)	1214, 2881
AjnI	CCWGG	5 cut(s)	1905, 2582, 2915, 3837, 4184
Alw21I	GWGCWC	3 cut(s)	144, 2556, 3621
Alw26I	GTCTC	5 cut(s)	1163, 1624, 2912, 3986, 4001
Alw44I	GTGCAC	1 cut(s)	140
Ama87I	CYCGRG	1 cut(s)	3138
AoxI	GGCC	8 cut(s)	244, 1922, 1975, 2335, 2913, 3000, 3443, 3667
ApaLI	GTGCAC	1 cut(s)	140
ApeKI	GCWGC	7 cut(s)	845, 1037, 1052, 2159, 3281, 3757, 3889
Asp700I	GAANNNNTTC	1 cut(s)	192
AspLEI	GCGC	1 cut(s)	2758
AspS9I	GGNCC	5 cut(s)	244, 674, 896, 2336, 3500
AsuHPI	GGTGA	8 cut(s)	1308, 2129, 2252, 2602, 3247, 3334, 4021, 4031
AvaI	CYCGRG	1 cut(s)	3138
AvaII	GGWCC	3 cut(s)	674, 896, 3500
AxyI	CCTNAGG	1 cut(s)	3495
BaeGI	GKGCMC	2 cut(s)	19, 144
BalI	TGGCCA	2 cut(s)	3002, 3445
BamHI	GGATCC	2 cut(s)	1812, 3748
BanI	GGYRCC	1 cut(s)	3403
BanII	GRGCYC	1 cut(s)	3621
BauI	CACGAG	2 cut(s)	1533, 2909
BbsI	GAAGAC	3 cut(s)	333, 2656, 2799
Bbv12I	GWGCWC	3 cut(s)	144, 2556, 3621
BbvI	GCAGC	7 cut(s)	857, 1024, 1064, 2171, 3268, 3769, 3901
BccI	CCATC	6 cut(s)	231, 388, 520, 2180, 2554, 3854
BcgI	CGANNNNNNTGC	2 cut(s)	314, 348
BciT130I	CCWGG	5 cut(s)	1907, 2584, 2917, 3839, 4186
BclI	TGATCA	1 cut(s)	381
BcoDI	GTCTC	5 cut(s)	1163, 1624, 2912, 3986, 4001
BcuI	ACTAGT	2 cut(s)	1214, 2881
BfmI	CTRYAG	8 cut(s)	1164, 1323, 2011, 2055, 3311, 3671, 3755, 3960
BfoI	RGCGCY	1 cut(s)	2759
BglII	AGATCT	1 cut(s)	4178
BisI	GCNGC	8 cut(s)	846, 1038, 1053, 1290, 2160, 3282, 3758, 3890
BlsI	GCNGC	8 cut(s)	847, 1039, 1054, 1291, 2161, 3283, 3759, 3891
BmcAI	AGTACT	3 cut(s)	403, 890, 2975
Bme1390I	CCNGG	5 cut(s)	1907, 2584, 2917, 3839, 4186
Bme18I	GGWCC	3 cut(s)	674, 896, 3500
BmeT110I	CYCGRG	1 cut(s)	3138
BmgT120I	GGNCC	5 cut(s)	244, 674, 896, 2336, 3500
BmiI	GGNNCC	8 cut(s)	676, 1814, 1825, 2338, 3027, 3405, 3501, 3750
BmrFI	CCNGG	5 cut(s)	1907, 2584, 2917, 3839, 4186
BpiI	GAAGAC	3 cut(s)	333, 2656, 2799
BplI	GAGNNNNNCTC	2 cut(s)	1436, 1468
BpmI	CTGGAG	2 cut(s)	2540, 3892
Bpu10I	CCTNAGC	1 cut(s)	1705
BpuEI	CTTGAG	3 cut(s)	500, 3754, 4192
Bsa29I	ATCGAT	1 cut(s)	3097
BsaAI	YACGTR	2 cut(s)	20, 36
BsaI	GGTCTC	1 cut(s)	1624
BsaJI	CCNNGG	5 cut(s)	247, 1284, 1808, 2583, 4032
BsaWI	WCCGGW	3 cut(s)	2305, 2406, 4041
BsaXI	ACNNNNNCTCC	4 cut(s)	966, 996, 1101, 1131
Bsc4I	CCNNNNNNNGG	8 cut(s)	493, 603, 905, 1048, 2406, 2574, 3268, 3452
Bse21I	CCTNAGG	1 cut(s)	3495
Bse3DI	GCAATG	5 cut(s)	780, 910, 1621, 3120, 3346
BseBI	CCWGG	5 cut(s)	1907, 2584, 2917, 3839, 4186
BseCI	ATCGAT	1 cut(s)	3097
BseDI	CCNNGG	5 cut(s)	247, 1284, 1808, 2583, 4032
BseGI	GGATG	6 cut(s)	459, 662, 940, 1556, 2739, 3847
BseLI	CCNNNNNNNGG	8 cut(s)	493, 603, 905, 1048, 2406, 2574, 3268, 3452
BseMI	GCAATG	5 cut(s)	780, 910, 1621, 3120, 3346
BseRI	GAGGAG	1 cut(s)	4189
BseSI	GKGCMC	2 cut(s)	19, 144
BseXI	GCAGC	7 cut(s)	857, 1024, 1064, 2171, 3268, 3769, 3901
BseYI	CCCAGC	1 cut(s)	2715
BsgI	GTGCAG	1 cut(s)	2247
Bsh1236I	CGCG	1 cut(s)	2460
Bsh1285I	CGRYCG	1 cut(s)	2306
BshFI	GGCC	8 cut(s)	246, 1924, 1977, 2337, 2915, 3002, 3445, 3669
BshNI	GGYRCC	1 cut(s)	3403
BshVI	ATCGAT	1 cut(s)	3097
BsiEI	CGRYCG	1 cut(s)	2306
BsiHKAI	GWGCWC	3 cut(s)	144, 2556, 3621
BsiHKCI	CYCGRG	1 cut(s)	3138
BsiSI	CCGG	3 cut(s)	2306, 2407, 4042
BslFI	GGGAC	5 cut(s)	265, 489, 660, 1847, 2911
BslI	CCNNNNNNNGG	8 cut(s)	493, 603, 905, 1048, 2406, 2574, 3268, 3452
BsmAI	GTCTC	5 cut(s)	1163, 1624, 2912, 3986, 4001
BsmFI	GGGAC	5 cut(s)	265, 489, 660, 1847, 2911
BsmI	GAATGC	1 cut(s)	4022
BsnI	GGCC	8 cut(s)	246, 1924, 1977, 2337, 2915, 3002, 3445, 3669
Bso31I	GGTCTC	1 cut(s)	1624
BsoBI	CYCGRG	1 cut(s)	3138
Bsp1286I	GDGCHC	4 cut(s)	19, 144, 2556, 3621
Bsp1407I	TGTACA	2 cut(s)	1020, 1278
Bsp19I	CCATGG	2 cut(s)	247, 1808
BspACI	CCGC	5 cut(s)	1289, 1692, 3704, 4143, 4163
BspANI	GGCC	8 cut(s)	246, 1924, 1977, 2337, 2915, 3002, 3445, 3669
BspDI	ATCGAT	1 cut(s)	3097
BspFNI	CGCG	1 cut(s)	2460
BspHI	TCATGA	1 cut(s)	1356
BspLI	GGNNCC	8 cut(s)	676, 1814, 1825, 2338, 3027, 3405, 3501, 3750
BspMAI	CTGCAG	4 cut(s)	1168, 3315, 3675, 3759
BspT107I	GGYRCC	1 cut(s)	3403
BspTNI	GGTCTC	1 cut(s)	1624
BsrDI	GCAATG	5 cut(s)	780, 910, 1621, 3120, 3346
BsrGI	TGTACA	2 cut(s)	1020, 1278
BssECI	CCNNGG	5 cut(s)	247, 1284, 1808, 2583, 4032
BssNAI	GTATAC	1 cut(s)	1733
BssSI	CACGAG	2 cut(s)	1533, 2909
BssT1I	CCWWGG	3 cut(s)	247, 1284, 1808
Bst1107I	GTATAC	1 cut(s)	1733
Bst2BI	CACGAG	2 cut(s)	1533, 2909
Bst2UI	CCWGG	5 cut(s)	1907, 2584, 2917, 3839, 4186
Bst6I	CTCTTC	6 cut(s)	207, 586, 967, 2549, 3917, 4144
BstAPI	GCANNNNNTGC	1 cut(s)	641
BstAUI	TGTACA	2 cut(s)	1020, 1278
BstBAI	YACGTR	2 cut(s)	20, 36
BstC8I	GCNNGC	6 cut(s)	263, 753, 1764, 2656, 3315, 3671
BstDSI	CCRYGG	3 cut(s)	247, 1808, 4032
BstF5I	GGATG	6 cut(s)	459, 662, 940, 1556, 2739, 3847
BstFNI	CGCG	1 cut(s)	2460
BstH2I	RGCGCY	1 cut(s)	2759
BstHHI	GCGC	1 cut(s)	2758
BstMAI	GTCTC	5 cut(s)	1163, 1624, 2912, 3986, 4001
BstMCI	CGRYCG	1 cut(s)	2306
BstMWI	GCNNNNNNNGC	9 cut(s)	267, 542, 632, 641, 1716, 1725, 2060, 2762, 3278
BstNI	CCWGG	5 cut(s)	1907, 2584, 2917, 3839, 4186
BstNSI	RCATGY	3 cut(s)	27, 43, 736
BstSCI	CCNGG	5 cut(s)	1905, 2582, 2915, 3837, 4184
BstSFI	CTRYAG	8 cut(s)	1164, 1323, 2011, 2055, 3311, 3671, 3755, 3960
BstSLI	GKGCMC	2 cut(s)	19, 144
BstUI	CGCG	1 cut(s)	2460
BstV1I	GCAGC	7 cut(s)	857, 1024, 1064, 2171, 3268, 3769, 3901
BstV2I	GAAGAC	3 cut(s)	333, 2656, 2799
BstX2I	RGATCY	6 cut(s)	483, 1812, 2230, 2424, 3748, 4178
BstXI	CCANNNNNNTGG	1 cut(s)	710
BstYI	RGATCY	6 cut(s)	483, 1812, 2230, 2424, 3748, 4178
BstZ17I	GTATAC	1 cut(s)	1733
Bsu15I	ATCGAT	1 cut(s)	3097
Bsu36I	CCTNAGG	1 cut(s)	3495
BsuRI	GGCC	8 cut(s)	246, 1924, 1977, 2337, 2915, 3002, 3445, 3669
BsuTUI	ATCGAT	1 cut(s)	3097
BtgI	CCRYGG	3 cut(s)	247, 1808, 4032
BtsCI	GGATG	6 cut(s)	459, 662, 940, 1556, 2739, 3847
BtsI	GCAGTG	2 cut(s)	1299, 2985
Cac8I	GCNNGC	6 cut(s)	263, 753, 1764, 2656, 3315, 3671
CciI	TCATGA	1 cut(s)	1356
CfoI	GCGC	1 cut(s)	2758
Cfr13I	GGNCC	5 cut(s)	244, 674, 896, 2336, 3500
ClaI	ATCGAT	1 cut(s)	3097
CseI	GACGC	1 cut(s)	3865
DraI	TTTAAA	1 cut(s)	469
DraIII	CACNNNGTG	1 cut(s)	106
EaeI	YGGCCR	2 cut(s)	3000, 3443
Eam1104I	CTCTTC	6 cut(s)	207, 586, 967, 2549, 3917, 4144
EarI	CTCTTC	6 cut(s)	207, 586, 967, 2549, 3917, 4144
Ecl136II	GAGCTC	1 cut(s)	3619
Eco130I	CCWWGG	3 cut(s)	247, 1284, 1808
Eco147I	AGGCCT	1 cut(s)	3669
Eco24I	GRGCYC	1 cut(s)	3621
Eco31I	GGTCTC	1 cut(s)	1624
Eco32I	GATATC	2 cut(s)	2839, 3360
Eco47I	GGWCC	3 cut(s)	674, 896, 3500
Eco53kI	GAGCTC	1 cut(s)	3619
Eco57I	CTGAAG	5 cut(s)	510, 2727, 3471, 3876, 3938
Eco81I	CCTNAGG	1 cut(s)	3495
Eco88I	CYCGRG	1 cut(s)	3138
EcoICRI	GAGCTC	1 cut(s)	3619
EcoRI	GAATTC	6 cut(s)	66, 953, 1488, 1496, 3372, 4166
EcoRII	CCWGG	5 cut(s)	1905, 2582, 2915, 3837, 4184
EcoRV	GATATC	2 cut(s)	2839, 3360
EcoT14I	CCWWGG	3 cut(s)	247, 1284, 1808
EcoT38I	GRGCYC	1 cut(s)	3621
ErhI	CCWWGG	3 cut(s)	247, 1284, 1808
FalI	AAGNNNNNCTT	4 cut(s)	740, 772, 863, 895
FaqI	GGGAC	5 cut(s)	265, 489, 660, 1847, 2911
FauI	CCCGC	1 cut(s)	3711
FauNDI	CATATG	1 cut(s)	3289
FbaI	TGATCA	1 cut(s)	381
FblI	GTMKAC	5 cut(s)	832, 1732, 2010, 2509, 3802
Fnu4HI	GCNGC	8 cut(s)	846, 1038, 1053, 1290, 2160, 3282, 3758, 3890
FokI	GGATG	6 cut(s)	466, 649, 947, 1543, 2746, 3854
FriOI	GRGCYC	1 cut(s)	3621
Fsp4HI	GCNGC	8 cut(s)	846, 1038, 1053, 1290, 2160, 3282, 3758, 3890
GlaI	GCGC	1 cut(s)	2757
GluI	GCNGC	8 cut(s)	846, 1038, 1053, 1290, 2160, 3282, 3758, 3890
GsaI	CCCAGC	1 cut(s)	2719
GsuI	CTGGAG	2 cut(s)	2540, 3892
HaeII	RGCGCY	1 cut(s)	2759
HaeIII	GGCC	8 cut(s)	246, 1924, 1977, 2337, 2915, 3002, 3445, 3669
HapII	CCGG	3 cut(s)	2306, 2407, 4042
HgaI	GACGC	1 cut(s)	3865
HhaI	GCGC	1 cut(s)	2758
Hin6I	GCGC	1 cut(s)	2756
HinP1I	GCGC	1 cut(s)	2756
HincII	GTYRAC	3 cut(s)	2300, 2830, 3803
HindII	GTYRAC	3 cut(s)	2300, 2830, 3803
HindIII	AAGCTT	5 cut(s)	339, 749, 2391, 3420, 3730
HpaII	CCGG	3 cut(s)	2306, 2407, 4042
HphI	GGTGA	8 cut(s)	1308, 2129, 2252, 2602, 3247, 3334, 4021, 4031
Hpy99I	CGWCG	2 cut(s)	2948, 3881
HpyCH4IV	ACGT	5 cut(s)	19, 35, 2538, 3300, 3474
HpyF10VI	GCNNNNNNNGC	9 cut(s)	267, 542, 632, 641, 1716, 1725, 2060, 2762, 3278
HpySE526I	ACGT	5 cut(s)	19, 35, 2538, 3300, 3474
HspAI	GCGC	1 cut(s)	2756
Ksp22I	TGATCA	1 cut(s)	381
LmnI	GCTCC	9 cut(s)	432, 561, 1236, 1522, 2559, 3031, 3062, 3840, 3873
Lsp1109I	GCAGC	7 cut(s)	857, 1024, 1064, 2171, 3268, 3769, 3901
MaeII	ACGT	5 cut(s)	19, 35, 2538, 3300, 3474
MaeIII	GTNAC	7 cut(s)	410, 1327, 2488, 2534, 2608, 3301, 4064
MfeI	CAATTG	2 cut(s)	2921, 3054
MflI	RGATCY	6 cut(s)	483, 1812, 2230, 2424, 3748, 4178
MhlI	GDGCHC	4 cut(s)	19, 144, 2556, 3621
MlsI	TGGCCA	2 cut(s)	3002, 3445
MluNI	TGGCCA	2 cut(s)	3002, 3445
MlyI	GAGTC	5 cut(s)	1165, 1197, 1595, 1695, 2768
MmeI	TCCRAC	3 cut(s)	691, 928, 3859
Mox20I	TGGCCA	2 cut(s)	3002, 3445
MroXI	GAANNNNTTC	1 cut(s)	192
MscI	TGGCCA	2 cut(s)	3002, 3445
MslI	CAYNNNNRTG	8 cut(s)	22, 38, 417, 448, 731, 1358, 2255, 3156
Msp20I	TGGCCA	2 cut(s)	3002, 3445
MspI	CCGG	3 cut(s)	2306, 2407, 4042
MspR9I	CCNGG	5 cut(s)	1907, 2584, 2917, 3839, 4186
MunI	CAATTG	2 cut(s)	2921, 3054
Mva1269I	GAATGC	1 cut(s)	4022
MvaI	CCWGG	5 cut(s)	1907, 2584, 2917, 3839, 4186
MvnI	CGCG	1 cut(s)	2460
MwoI	GCNNNNNNNGC	9 cut(s)	267, 542, 632, 641, 1716, 1725, 2060, 2762, 3278
NcoI	CCATGG	2 cut(s)	247, 1808
NdeI	CATATG	1 cut(s)	3289
NlaIV	GGNNCC	8 cut(s)	676, 1814, 1825, 2338, 3027, 3405, 3501, 3750
NmeAIII	GCCGAG	2 cut(s)	799, 4099
NmuCI	GTSAC	5 cut(s)	410, 2488, 2534, 2608, 4064
NspI	RCATGY	3 cut(s)	27, 43, 736
PaeR7I	CTCGAG	1 cut(s)	3138
PagI	TCATGA	1 cut(s)	1356
PceI	AGGCCT	1 cut(s)	3669
PciI	ACATGT	2 cut(s)	23, 732
PcsI	WCGNNNNNNNCGW	2 cut(s)	61, 321
PctI	GAATGC	1 cut(s)	4022
PdmI	GAANNNNTTC	1 cut(s)	192
PflMI	CCANNNNNTGG	3 cut(s)	493, 905, 2574
PfoI	TCCNGGA	2 cut(s)	3837, 4184
PkrI	GCNGC	8 cut(s)	847, 1039, 1054, 1291, 2161, 3283, 3759, 3891
PleI	GAGTC	5 cut(s)	1165, 1196, 1594, 1694, 2768
PpsI	GAGTC	5 cut(s)	1165, 1196, 1594, 1694, 2768
Ppu21I	YACGTR	2 cut(s)	20, 36
PscI	ACATGT	2 cut(s)	23, 732
PsiI	TTATAA	1 cut(s)	1875
Psp124BI	GAGCTC	1 cut(s)	3621
Psp1406I	AACGTT	1 cut(s)	3300
Psp6I	CCWGG	5 cut(s)	1905, 2582, 2915, 3837, 4184
PspFI	CCCAGC	1 cut(s)	2715
PspGI	CCWGG	5 cut(s)	1905, 2582, 2915, 3837, 4184
PspN4I	GGNNCC	8 cut(s)	676, 1814, 1825, 2338, 3027, 3405, 3501, 3750
PspPI	GGNCC	5 cut(s)	244, 674, 896, 2336, 3500
PsrI	GAACNNNNNNTAC	2 cut(s)	2235, 2267
PstI	CTGCAG	4 cut(s)	1168, 3315, 3675, 3759
PsuI	RGATCY	6 cut(s)	483, 1812, 2230, 2424, 3748, 4178
RseI	CAYNNNNRTG	8 cut(s)	22, 38, 417, 448, 731, 1358, 2255, 3156
SacI	GAGCTC	1 cut(s)	3621
SalI	GTCGAC	1 cut(s)	3801
SatI	GCNGC	8 cut(s)	846, 1038, 1053, 1290, 2160, 3282, 3758, 3890
Sau96I	GGNCC	5 cut(s)	244, 674, 896, 2336, 3500
SbfI	CCTGCAGG	2 cut(s)	3315, 3675
ScaI	AGTACT	3 cut(s)	403, 890, 2975
SchI	GAGTC	5 cut(s)	1165, 1197, 1595, 1695, 2768
ScrFI	CCNGG	5 cut(s)	1907, 2584, 2917, 3839, 4186
SdaI	CCTGCAGG	2 cut(s)	3315, 3675
SduI	GDGCHC	4 cut(s)	19, 144, 2556, 3621
SfcI	CTRYAG	8 cut(s)	1164, 1323, 2011, 2055, 3311, 3671, 3755, 3960
Sfr274I	CTCGAG	1 cut(s)	3138
SinI	GGWCC	3 cut(s)	674, 896, 3500
SlaI	CTCGAG	1 cut(s)	3138
SmiMI	CAYNNNNRTG	8 cut(s)	22, 38, 417, 448, 731, 1358, 2255, 3156
SmlI	CTYRAG	4 cut(s)	479, 3138, 3733, 4171
SmoI	CTYRAG	4 cut(s)	479, 3138, 3733, 4171
SpeI	ACTAGT	2 cut(s)	1214, 2881
Sse8387I	CCTGCAGG	2 cut(s)	3315, 3675
SseBI	AGGCCT	1 cut(s)	3669
SsiI	CCGC	5 cut(s)	1289, 1692, 3704, 4143, 4163
SspI	AATATT	2 cut(s)	154, 3780
SstI	GAGCTC	1 cut(s)	3621
StuI	AGGCCT	1 cut(s)	3669
StyD4I	CCNGG	5 cut(s)	1905, 2582, 2915, 3837, 4184
StyI	CCWWGG	3 cut(s)	247, 1284, 1808
TaiI	ACGT	5 cut(s)	22, 38, 2541, 3303, 3477
TatI	WGTACW	6 cut(s)	401, 888, 1020, 1278, 1382, 2973
TauI	GCSGC	1 cut(s)	1292
TseFI	GTSAC	5 cut(s)	410, 2488, 2534, 2608, 4064
TseI	GCWGC	7 cut(s)	845, 1037, 1052, 2159, 3281, 3757, 3889
Tsp45I	GTSAC	5 cut(s)	410, 2488, 2534, 2608, 4064
TspGWI	ACGGA	1 cut(s)	1944
Van91I	CCANNNNNTGG	3 cut(s)	493, 905, 2574
VneI	GTGCAC	1 cut(s)	140
VpaK11BI	GGWCC	3 cut(s)	674, 896, 3500
XceI	RCATGY	3 cut(s)	27, 43, 736
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1291
XhoI	CTCGAG	1 cut(s)	3138
XmiI	GTMKAC	5 cut(s)	832, 1732, 2010, 2509, 3802
XmnI	GAANNNNTTC	1 cut(s)	192
ZrmI	AGTACT	3 cut(s)	403, 890, 2975

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.