MD13G1054900.v1.1

AhpC/TSA antioxidant enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0014726
Physical Location & Seq
Reverse (-)
3850291 .. 3853015
2725 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1054900.v1.1.491

Sequence Viewer

Length: 1260 bp
ATGGCTTCTTTTTCGATAGAGGAGTTTGTTGGGGATGGGGTTCTGAAAGGAATGATTCCGAAGCTGTTAGACGAAGGCTGGGATGATGTACCGACCTTGAAGATCATGAATACTGATGATATGGATGCATTAAACATGACACAACTGGAGAAGGATGCATTGGAAATTAGGTCATACCTGCACGACCGTGCTTTGATGCAATATGGAGATAGGCTCGAGACCAGTGGGAAATGTCTGTCCGAGCTAATTAGCTTAAGCACTGTAGATCTTTCTTCTCAGTTTGGCATGAAGCGTGGTCATATTGCCCGTTTCATGGACAGAACTAGTGCTTGTGCAGATCACCTACCAAAGTCTCATCCTACCCCTGCTGCAACAGAAAGAATGGGCTTACCGTCGAAAAATACTAGCATTCAAAGGAGTTTTCAATCTGTTAACTCTCAAAAGATCCAGAGTAAATTAACAACAAGAACTAGTTCAATTAACAGCAAATCTCTAGAACAATCACTGGCAAGTTTTAAGATCAAAGATGGATACATCTTCAAAGGGATCGTTGCTATGGAGCCAGCCGATCCTAGATTATGTGGTTGTATACAACCTGCTCCTGTAGTTGACAAAGTTGCACCATACTCTACCATTGAAAACATCTCGGTTCAGAAGTTGACACCAGAGTATAAGATTGGAATGGAGCATTTGGTGAAAACCAAAACACCTCCGATGAAGGCTTCAGAACTGTGGAGAAACAAACCAGCAGTGATTCTCTGCATTCGACGTCCAGGGTGCATCATGTGTAGGGCAGAAGCTCACCAGCTATATGCCAAAAAACCCATATTTGATTCACTGGGAGTTCAACTATTTGCAGTTCTTCATGAGCATTTGGAGTCAGAGATAAAAGACTTCTGGCCTCGATATTGGGGTGGTGTTGTATTGTATGATCGGGGAATGGAATTCTTCAAAGCACTTGGTGGAGGGAAACTGCTCAAGGAAAAATTCCTGTCAGGTTTTGTTTTCAACCCTCGAGCAAGAGCAAATTACAAGCGTGCAAAAGCTATGGGGATACAGCAAAACTTCAAAGGAGAAGGTGAAATTAAGGGGGGGCTTTTTGTAGTTGGCGGTGGAAGGAGTGGCATTGCATACCAGTTTATTGAGAGGAACTTCGGTGATTGGGCACCTCCAGCTGAAGTTATTGAGATCTGTGGTCAGTTGCAGAACCAACAAGAAGCTCAAGATGACTCCTACAAGAGATCACAGCAGTCCGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AhpC-TSA_2

Protein Analysis

420

Amino Acids

46.89

Weight (kDa)

8.73

Isoelectric Point (pI)

41.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AhpC-TSA_2 PF13911 269 - 384 1.4e-22 AhpC/TSA antioxidant enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014726)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g28930 FvH4_4g28930 FvH4_4g28930 FvH4_4g28930 FvH4_4g28930
malus_domestica MD13G1054900.v1.1 MD16G1053800.v1.1
rosa_chinensis RchiOBHm_Chr4g0437071
rosa_laevigata RLG00000006471
rosa_multiflora Rmu_ssc0000006.1_g000012
rosa_roxburghii Rroxscaffold_5G00378150
rosa_rugosa Rorug04G0298000
rosa_samantha Rh4AG352600 Rh4BG361900 Rh4CG376200 Rh4DG356100
rosa_wichuraiana Rw4G030870

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 772
Acc36I ACCTGC 2 cut(s) 186, 604
AccB1I GGYRCC 1 cut(s) 1165
AccI GTMKAC 1 cut(s) 589
AciI CCGC 1 cut(s) 1110
AclWI GGATC 3 cut(s) 439, 554, 563
AcsI RAATTY 2 cut(s) 944, 986
AcuI CTGAAG 2 cut(s) 708, 1197
AcyI GRCGYC 1 cut(s) 769
AdeI CACNNNGTG 1 cut(s) 962
AfaI GTAC 1 cut(s) 90
AfiI CCNNNNNNNGG 1 cut(s) 313
AflII CTTAAG 1 cut(s) 253
AhlI ACTAGT 2 cut(s) 323, 470
AjnI CCWGG 1 cut(s) 772
AjuI GAANNNNNNNTTGG 2 cut(s) 143, 175
AleI CACNNNNGTG 1 cut(s) 186
AluBI AGCT 8 cut(s) 64, 244, 252, 800, 808, 1046, 1175, 1220
AluI AGCT 8 cut(s) 64, 244, 252, 800, 808, 1046, 1175, 1220
Alw26I GTCTC 2 cut(s) 212, 357
AlwI GGATC 3 cut(s) 439, 554, 563
Ama87I CYCGRG 2 cut(s) 215, 1014
AoxI GGCC 1 cut(s) 899
ApeKI GCWGC 1 cut(s) 368
ApoI RAATTY 2 cut(s) 944, 986
Asp700I GAANNNNTTC 1 cut(s) 472
AsuHPI GGTGA 5 cut(s) 332, 706, 794, 1091, 1169
AvaI CYCGRG 2 cut(s) 215, 1014
BaeGI GKGCMC 1 cut(s) 1168
BanI GGYRCC 1 cut(s) 1165
BbvI GCAGC 1 cut(s) 355
BccI CCATC 2 cut(s) 29, 521
BciT130I CCWGG 1 cut(s) 774
BciVI GTATCC 2 cut(s) 524, 1047
BcoDI GTCTC 2 cut(s) 212, 357
BcuI ACTAGT 2 cut(s) 323, 470
BfaI CTAG 5 cut(s) 324, 405, 471, 494, 573
BfmI CTRYAG 2 cut(s) 261, 603
BfrI CTTAAG 1 cut(s) 253
BfuAI ACCTGC 2 cut(s) 186, 604
BfuI GTATCC 2 cut(s) 524, 1047
BglII AGATCT 2 cut(s) 265, 1188
BisI GCNGC 1 cut(s) 369
BlsI GCNGC 1 cut(s) 370
Bme1390I CCNGG 1 cut(s) 774
BmeT110I CYCGRG 2 cut(s) 215, 1014
BmiI GGNNCC 2 cut(s) 561, 1167
BmrFI CCNGG 1 cut(s) 774
BmrI ACTGGG 1 cut(s) 848
BmsI GCATC 4 cut(s) 115, 145, 186, 789
BmuI ACTGGG 1 cut(s) 848
BplI GAGNNNNNCTC 2 cut(s) 198, 230
BpmI CTGGAG 2 cut(s) 167, 1155
BpuEI CTTGAG 2 cut(s) 962, 1206
BsaHI GRCGYC 1 cut(s) 769
BsaI GGTCTC 1 cut(s) 212
BsaJI CCNNGG 1 cut(s) 773
Bsc4I CCNNNNNNNGG 1 cut(s) 313
Bse1I ACTGG 5 cut(s) 150, 222, 510, 843, 1135
Bse3DI GCAATG 1 cut(s) 1125
BseBI CCWGG 1 cut(s) 774
BseDI CCNNGG 1 cut(s) 773
BseGI GGATG 5 cut(s) 40, 88, 130, 160, 355
BseLI CCNNNNNNNGG 1 cut(s) 313
BseMI GCAATG 1 cut(s) 1125
BseMII CTCAG 1 cut(s) 290
BseNI ACTGG 5 cut(s) 150, 222, 510, 843, 1135
BseRI GAGGAG 1 cut(s) 35
BseSI GKGCMC 1 cut(s) 1168
BseXI GCAGC 1 cut(s) 355
BseYI CCCAGC 1 cut(s) 78
BsgI GTGCAG 2 cut(s) 164, 354
Bsh1285I CGRYCG 1 cut(s) 187
BshFI GGCC 1 cut(s) 901
BshNI GGYRCC 1 cut(s) 1165
BsiEI CGRYCG 1 cut(s) 187
BsiHKCI CYCGRG 2 cut(s) 215, 1014
BslI CCNNNNNNNGG 1 cut(s) 313
BsmAI GTCTC 2 cut(s) 212, 357
BsmI GAATGC 2 cut(s) 408, 762
BsnI GGCC 1 cut(s) 901
Bso31I GGTCTC 1 cut(s) 212
BsoBI CYCGRG 2 cut(s) 215, 1014
Bsp1286I GDGCHC 1 cut(s) 1168
BspACI CCGC 1 cut(s) 1110
BspANI GGCC 1 cut(s) 901
BspCNI CTCAG 1 cut(s) 289
BspHI TCATGA 2 cut(s) 105, 865
BspLI GGNNCC 2 cut(s) 561, 1167
BspMI ACCTGC 2 cut(s) 186, 604
BspPI GGATC 3 cut(s) 439, 554, 563
BspT107I GGYRCC 1 cut(s) 1165
BspTI CTTAAG 1 cut(s) 253
BspTNI GGTCTC 1 cut(s) 212
BsrDI GCAATG 1 cut(s) 1125
BsrI ACTGG 5 cut(s) 150, 222, 510, 843, 1135
BssECI CCNNGG 1 cut(s) 773
BssNAI GTATAC 1 cut(s) 590
BssNI GRCGYC 1 cut(s) 769
Bst1107I GTATAC 1 cut(s) 590
Bst2UI CCWGG 1 cut(s) 774
Bst4CI ACNGT 4 cut(s) 188, 262, 393, 732
BstACI GRCGYC 1 cut(s) 769
BstAFI CTTAAG 1 cut(s) 253
BstC8I GCNNGC 2 cut(s) 564, 1038
BstDEI CTNAG 1 cut(s) 276
BstF5I GGATG 5 cut(s) 40, 88, 130, 160, 355
BstMAI GTCTC 2 cut(s) 212, 357
BstMCI CGRYCG 1 cut(s) 187
BstMWI GCNNNNNNNGC 1 cut(s) 1172
BstNI CCWGG 1 cut(s) 774
BstSCI CCNGG 1 cut(s) 772
BstSFI CTRYAG 2 cut(s) 261, 603
BstSLI GKGCMC 1 cut(s) 1168
BstV1I GCAGC 1 cut(s) 355
BstX2I RGATCY 3 cut(s) 265, 444, 1188
BstYI RGATCY 3 cut(s) 265, 444, 1188
BstZ17I GTATAC 1 cut(s) 590
BsuI GTATCC 2 cut(s) 524, 1047
BsuRI GGCC 1 cut(s) 901
BtsCI GGATG 5 cut(s) 40, 88, 130, 160, 355
BtsI GCAGTG 1 cut(s) 756
BtsIMutI CAGTG 5 cut(s) 229, 258, 503, 756, 836
BveI ACCTGC 2 cut(s) 186, 604
Cac8I GCNNGC 2 cut(s) 564, 1038
CciI TCATGA 2 cut(s) 105, 865
Csp6I GTAC 1 cut(s) 89
CviAII CATG 6 cut(s) 106, 136, 286, 313, 784, 866
CviQI GTAC 1 cut(s) 89
DdeI CTNAG 1 cut(s) 276
DraIII CACNNNGTG 1 cut(s) 962
Eco31I GGTCTC 1 cut(s) 212
Eco57I CTGAAG 2 cut(s) 708, 1197
Eco88I CYCGRG 2 cut(s) 215, 1014
EcoRI GAATTC 1 cut(s) 944
EcoRII CCWGG 1 cut(s) 772
EcoT22I ATGCAT 2 cut(s) 130, 160
FaeI CATG 6 cut(s) 109, 139, 289, 316, 787, 869
FatI CATG 6 cut(s) 105, 135, 285, 312, 783, 865
FblI GTMKAC 1 cut(s) 589
Fnu4HI GCNGC 1 cut(s) 369
FokI GGATG 5 cut(s) 47, 95, 137, 167, 342
Fsp4HI GCNGC 1 cut(s) 369
FspBI CTAG 5 cut(s) 324, 405, 471, 494, 573
GluI GCNGC 1 cut(s) 369
GsaI CCCAGC 1 cut(s) 82
GsuI CTGGAG 2 cut(s) 167, 1155
HaeIII GGCC 1 cut(s) 901
Hin1I GRCGYC 1 cut(s) 769
Hin1II CATG 6 cut(s) 109, 139, 289, 316, 787, 869
HincII GTYRAC 3 cut(s) 433, 610, 660
HindII GTYRAC 3 cut(s) 433, 610, 660
HinfI GANTC 5 cut(s) 55, 754, 833, 878, 1229
HpaI GTTAAC 1 cut(s) 433
HphI GGTGA 5 cut(s) 332, 706, 794, 1091, 1169
Hpy166II GTNNAC 4 cut(s) 433, 590, 610, 660
Hpy188I TCNGA 8 cut(s) 45, 60, 241, 654, 714, 727, 883, 1255
Hpy188III TCNNGA 6 cut(s) 106, 217, 448, 494, 866, 1223
Hpy8I GTNNAC 4 cut(s) 433, 590, 610, 660
Hpy99I CGWCG 2 cut(s) 397, 771
HpyAV CCTTC 5 cut(s) 68, 145, 712, 1070, 1110
HpyCH4III ACNGT 4 cut(s) 188, 262, 393, 732
HpyCH4IV ACGT 1 cut(s) 769
HpyF10VI GCNNNNNNNGC 1 cut(s) 1172
HpyF3I CTNAG 1 cut(s) 276
HpySE526I ACGT 1 cut(s) 769
Hsp92I GRCGYC 1 cut(s) 769
Hsp92II CATG 6 cut(s) 109, 139, 289, 316, 787, 869
KspAI GTTAAC 1 cut(s) 433
LmnI GCTCC 3 cut(s) 559, 604, 685
Lsp1109I GCAGC 1 cut(s) 355
LweI GCATC 4 cut(s) 115, 145, 186, 789
MaeI CTAG 5 cut(s) 324, 405, 471, 494, 573
MaeII ACGT 1 cut(s) 769
MboII GAAGA 5 cut(s) 112, 264, 529, 854, 940
MflI RGATCY 3 cut(s) 265, 444, 1188
MhlI GDGCHC 1 cut(s) 1168
MluCI AATT 8 cut(s) 165, 246, 455, 477, 944, 986, 1027, 1083
MlyI GAGTC 2 cut(s) 887, 1223
MnlI CCTC 7 cut(s) 13, 720, 912, 959, 1023, 1140, 1179
Mph1103I ATGCAT 2 cut(s) 130, 160
MroXI GAANNNNTTC 1 cut(s) 472
MseI TTAA 7 cut(s) 131, 254, 432, 458, 480, 516, 1086
MslI CAYNNNNRTG 1 cut(s) 186
MspA1I CMGCKG 1 cut(s) 1175
MspCI CTTAAG 1 cut(s) 253
MspR9I CCNGG 1 cut(s) 774
Mva1269I GAATGC 2 cut(s) 408, 762
MvaI CCWGG 1 cut(s) 774
MwoI GCNNNNNNNGC 1 cut(s) 1172
NlaIII CATG 6 cut(s) 109, 139, 289, 316, 787, 869
NlaIV GGNNCC 2 cut(s) 561, 1167
NsiI ATGCAT 2 cut(s) 130, 160
OliI CACNNNNGTG 1 cut(s) 186
PaeR7I CTCGAG 2 cut(s) 215, 1014
PagI TCATGA 2 cut(s) 105, 865
PctI GAATGC 2 cut(s) 408, 762
PdmI GAANNNNTTC 1 cut(s) 472
PfeI GAWTC 3 cut(s) 55, 754, 833
PkrI GCNGC 1 cut(s) 370
PleI GAGTC 2 cut(s) 886, 1223
PpsI GAGTC 2 cut(s) 886, 1223
Psp6I CCWGG 1 cut(s) 772
PspFI CCCAGC 1 cut(s) 78
PspGI CCWGG 1 cut(s) 772
PspN4I GGNNCC 2 cut(s) 561, 1167
PspXI VCTCGAGB 1 cut(s) 1014
PsuI RGATCY 3 cut(s) 265, 444, 1188
PvuII CAGCTG 1 cut(s) 1175
RsaI GTAC 1 cut(s) 90
RsaNI GTAC 1 cut(s) 89
RseI CAYNNNNRTG 1 cut(s) 186
SaqAI TTAA 7 cut(s) 131, 254, 432, 458, 480, 516, 1086
SatI GCNGC 1 cut(s) 369
SchI GAGTC 2 cut(s) 887, 1223
ScrFI CCNGG 1 cut(s) 774
SduI GDGCHC 1 cut(s) 1168
SfaNI GCATC 4 cut(s) 115, 145, 186, 789
SfcI CTRYAG 2 cut(s) 261, 603
Sfr274I CTCGAG 2 cut(s) 215, 1014
SlaI CTCGAG 2 cut(s) 215, 1014
SmiMI CAYNNNNRTG 1 cut(s) 186
SmlI CTYRAG 5 cut(s) 215, 253, 977, 1014, 1221
SmoI CTYRAG 5 cut(s) 215, 253, 977, 1014, 1221
SpeI ACTAGT 2 cut(s) 323, 470
Sse9I AATT 8 cut(s) 165, 246, 455, 477, 944, 986, 1027, 1083
SsiI CCGC 1 cut(s) 1110
SspMI CTAG 5 cut(s) 324, 405, 471, 494, 573
StyD4I CCNGG 1 cut(s) 772
TaaI ACNGT 4 cut(s) 188, 262, 393, 732
TaiI ACGT 1 cut(s) 772
TaqI TCGA 6 cut(s) 14, 216, 395, 766, 904, 1015
TasI AATT 8 cut(s) 165, 246, 455, 477, 944, 986, 1027, 1083
TfiI GAWTC 3 cut(s) 55, 754, 833
Tru1I TTAA 7 cut(s) 131, 254, 432, 458, 480, 516, 1086
Tru9I TTAA 7 cut(s) 131, 254, 432, 458, 480, 516, 1086
TscAI CASTG 5 cut(s) 229, 265, 510, 756, 843
TseI GCWGC 1 cut(s) 368
TspDTI ATGAA 5 cut(s) 122, 301, 302, 731, 854
TspRI CASTG 5 cut(s) 229, 265, 510, 756, 843
Vha464I CTTAAG 1 cut(s) 253
XapI RAATTY 2 cut(s) 944, 986
XbaI TCTAGA 1 cut(s) 493
XhoI CTCGAG 2 cut(s) 215, 1014
XmiI GTMKAC 1 cut(s) 589
XmnI GAANNNNTTC 1 cut(s) 472
XspI CTAG 5 cut(s) 324, 405, 471, 494, 573
ZraI GACGTC 1 cut(s) 770
Zsp2I ATGCAT 2 cut(s) 130, 160
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.