MD16G1053800.v1.1

AhpC/TSA antioxidant enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0014726
Physical Location & Seq
Reverse (-)
3809591 .. 3811667
2077 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1053800.v1.1.491

Sequence Viewer

Length: 1437 bp
ATGTCGACTCTTGCGCAGCTGAGCCTAACAGCAATAGGGTTTTCATCTCTACATCCAGACTCGATTGAGCGAGCACTGATTCGTTTGGAACATTCTCTGGTTTCTTCGAAGATGGCTTCTTTTTCGATAGAGGAGTTTGTTGGGGACGGGGTTCCGAAAGGACTGATTCCAAAGCTGTTAGATGAAGGCTGGGATGATGTACCGACCTTGAAAATCATGAATACAGATGATATGGATGCATTAAACATGACAGAACCGGAGAAGGATGCATTGGAAATCAGGTCTTACCTGCACGACCGAGCTTTGATGCAATACGGAGATAGGCTCGAGTCCAGTGGGAAATGTCTGTCTGAGCTCCTTAACTTAAGCACTGTACAGCTTTCTTCTCAGTTTGGCATGAAGCGAGGCCACATTGCCCGTTTCATGGACAGAACCAGTGCTTTTGCGGATCACCTACCAAAGTCTCATCCTACCCCTGCTGCAACAGGAAGAATGGGCTTACTATCGAAAAATACTAGCATTCAAAGGAGTTTTCAATCTGTAAATTCTCAAAAGATCCTGAGTAAATTACCAACAAGAACTAGTTCAATTAACGGAAAATCTCTAGAACAATCACTGGCAAGTTTCAAGATCAGGGATGGATACATCTTCAAAGGGATCGTTGCCATGGAGCCAGCTGAACCTAGAGCATGTGGTTGTGTACAACCTGATCCTGTAGCTGACAAAGTTGCTCCATACTCTACCATCGAAAACATCTCGGTTCAGAAGTTGACACCAGAGTATAAGATTGGAATGGAGCATTTGGTGAAAACCAAAATACCTCCGATGAAGGCTTCAGAACTGTGGAGAAACAAACCAGCAGTGATTCTCTGCATTCGACGTCCTGGGTGCATCATGTGTAGAGCTGAAGCTCACCAGCTATATGCCAAAAAACCGATATTTGATTCACTAGGGGTTCAACTATTTGCTGTTCTTCATGAGTATATGGAGTCAGAGATAAAAGACTTCTGGCCTCGATATTGGGGTGGTGTTGTATTGTATGATCGCGGAATGGAATTCTTCAAAGCACTTGGTGGAGGGAAACTGCTCAAGGAAAAATTCCTGTCAGGTTTTATTTTCAACCCTCGAGCTAGAGCAAATTACAAGCGTGCAAAAGCTATGGGGATACAGCAAAACTTCAAAGGAGAAGGTGAAATTAAGGGGGGGCTTTTTGTAGTTGGCGGTGGAAGGAGTGGCATTGCATACCAGTTTATTGAGAGGAACTTTGGTGATTGGGCACCTCCAGCTGAAGTCATTGAGATCTGTGCCCAGTTGAAGGTAATTCTTCATGGACTTTTGAATTCTTCCCAAGTCTGCATGAATGATAAACTTTATATACAAAAGCTCATCTTTAATGTTAACGCAGAACCAACAAGAAGCTCAAGAGGACTCCTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AhpC-TSA_2

Protein Analysis

479

Amino Acids

53.23

Weight (kDa)

9.12

Isoelectric Point (pI)

39.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AhpC-TSA_2 PF13911 306 - 421 7.9e-22 AhpC/TSA antioxidant enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014726)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g28930 FvH4_4g28930 FvH4_4g28930 FvH4_4g28930 FvH4_4g28930
malus_domestica MD13G1054900.v1.1 MD16G1053800.v1.1
rosa_chinensis RchiOBHm_Chr4g0437071
rosa_laevigata RLG00000006471
rosa_multiflora Rmu_ssc0000006.1_g000012
rosa_roxburghii Rroxscaffold_5G00378150
rosa_rugosa Rorug04G0298000
rosa_samantha Rh4AG352600 Rh4BG361900 Rh4CG376200 Rh4DG356100
rosa_wichuraiana Rw4G030870

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 883
Acc16I TGCGCA 1 cut(s) 15
Acc36I ACCTGC 1 cut(s) 297
AccB1I GGYRCC 1 cut(s) 1276
AccI GTMKAC 1 cut(s) 5
AccII CGCG 1 cut(s) 1047
AciI CCGC 3 cut(s) 446, 1047, 1221
AclWI GGATC 4 cut(s) 456, 550, 665, 704
AcsI RAATTY 4 cut(s) 544, 1055, 1097, 1339
AcuI CTGAAG 3 cut(s) 819, 927, 1308
AcyI GRCGYC 1 cut(s) 880
AdeI CACNNNGTG 1 cut(s) 1073
AfaI GTAC 3 cut(s) 201, 375, 702
AfiI CCNNNNNNNGG 2 cut(s) 424, 1315
AflII CTTAAG 1 cut(s) 364
AhlI ACTAGT 1 cut(s) 581
AjnI CCWGG 1 cut(s) 883
AjuI GAANNNNNNNTTGG 2 cut(s) 254, 286
Alw21I GWGCWC 2 cut(s) 76, 357
Alw26I GTCTC 1 cut(s) 468
AlwI GGATC 4 cut(s) 456, 550, 665, 704
Ama87I CYCGRG 2 cut(s) 326, 1125
AoxI GGCC 2 cut(s) 406, 1010
ApeKI GCWGC 2 cut(s) 16, 479
ApoI RAATTY 4 cut(s) 544, 1055, 1097, 1339
Asp700I GAANNNNTTC 1 cut(s) 583
AspLEI GCGC 1 cut(s) 16
AsuHPI GGTGA 5 cut(s) 443, 817, 905, 1202, 1280
AsuII TTCGAA 1 cut(s) 107
AvaI CYCGRG 2 cut(s) 326, 1125
BaeGI GKGCMC 2 cut(s) 1279, 1309
BanI GGYRCC 1 cut(s) 1276
BanII GRGCYC 1 cut(s) 357
Bbv12I GWGCWC 2 cut(s) 76, 357
BbvI GCAGC 2 cut(s) 28, 466
BccI CCATC 3 cut(s) 106, 632, 752
BciT130I CCWGG 1 cut(s) 885
BciVI GTATCC 2 cut(s) 635, 1158
BcoDI GTCTC 1 cut(s) 468
BcuI ACTAGT 1 cut(s) 581
BfaI CTAG 6 cut(s) 516, 582, 605, 684, 950, 1131
BfmI CTRYAG 1 cut(s) 714
BfrI CTTAAG 1 cut(s) 364
BfuAI ACCTGC 1 cut(s) 297
BfuI GTATCC 2 cut(s) 635, 1158
BglII AGATCT 1 cut(s) 1299
BisI GCNGC 2 cut(s) 17, 480
BlpI GCTNAGC 1 cut(s) 20
BlsI GCNGC 2 cut(s) 18, 481
Bme1390I CCNGG 1 cut(s) 885
BmeT110I CYCGRG 2 cut(s) 326, 1125
BmiI GGNNCC 3 cut(s) 153, 672, 1278
BmrFI CCNGG 1 cut(s) 885
BmrI ACTGGG 1 cut(s) 1303
BmsI GCATC 4 cut(s) 226, 256, 297, 900
BmuI ACTGGG 1 cut(s) 1303
BplI GAGNNNNNCTC 2 cut(s) 309, 341
BpmI CTGGAG 1 cut(s) 1266
Bpu1102I GCTNAGC 1 cut(s) 20
Bpu14I TTCGAA 1 cut(s) 107
BpuEI CTTGAG 2 cut(s) 1073, 1405
BsaHI GRCGYC 1 cut(s) 880
BsaJI CCNNGG 2 cut(s) 666, 884
BsaWI WCCGGW 1 cut(s) 256
Bsc4I CCNNNNNNNGG 2 cut(s) 424, 1315
Bse1I ACTGG 5 cut(s) 333, 435, 621, 1246, 1309
Bse3DI GCAATG 2 cut(s) 411, 1236
BseBI CCWGG 1 cut(s) 885
BseDI CCNNGG 2 cut(s) 666, 884
BseGI GGATG 6 cut(s) 52, 199, 241, 271, 466, 643
BseLI CCNNNNNNNGG 2 cut(s) 424, 1315
BseMI GCAATG 2 cut(s) 411, 1236
BseMII CTCAG 4 cut(s) 11, 342, 401, 551
BseNI ACTGG 5 cut(s) 333, 435, 621, 1246, 1309
BseRI GAGGAG 1 cut(s) 146
BseSI GKGCMC 2 cut(s) 1279, 1309
BseXI GCAGC 2 cut(s) 28, 466
BseYI CCCAGC 1 cut(s) 189
BsgI GTGCAG 1 cut(s) 275
Bsh1236I CGCG 1 cut(s) 1047
Bsh1285I CGRYCG 1 cut(s) 298
BshFI GGCC 2 cut(s) 408, 1012
BshNI GGYRCC 1 cut(s) 1276
BsiEI CGRYCG 1 cut(s) 298
BsiHKAI GWGCWC 2 cut(s) 76, 357
BsiHKCI CYCGRG 2 cut(s) 326, 1125
BsiSI CCGG 1 cut(s) 257
BslFI GGGAC 1 cut(s) 158
BslI CCNNNNNNNGG 2 cut(s) 424, 1315
BsmAI GTCTC 1 cut(s) 468
BsmFI GGGAC 1 cut(s) 158
BsmI GAATGC 2 cut(s) 519, 873
BsnI GGCC 2 cut(s) 408, 1012
BsoBI CYCGRG 2 cut(s) 326, 1125
Bsp119I TTCGAA 1 cut(s) 107
Bsp1286I GDGCHC 4 cut(s) 76, 357, 1279, 1309
Bsp1407I TGTACA 2 cut(s) 373, 700
Bsp143I GATC 7 cut(s) 448, 555, 630, 657, 709, 1042, 1299
Bsp1720I GCTNAGC 1 cut(s) 20
Bsp19I CCATGG 1 cut(s) 666
BspACI CCGC 3 cut(s) 446, 1047, 1221
BspANI GGCC 2 cut(s) 408, 1012
BspCNI CTCAG 4 cut(s) 12, 343, 400, 552
BspFNI CGCG 1 cut(s) 1047
BspHI TCATGA 2 cut(s) 216, 976
BspLI GGNNCC 3 cut(s) 153, 672, 1278
BspMI ACCTGC 1 cut(s) 297
BspPI GGATC 4 cut(s) 456, 550, 665, 704
BspT104I TTCGAA 1 cut(s) 107
BspT107I GGYRCC 1 cut(s) 1276
BspTI CTTAAG 1 cut(s) 364
BsrDI GCAATG 2 cut(s) 411, 1236
BsrGI TGTACA 2 cut(s) 373, 700
BsrI ACTGG 5 cut(s) 333, 435, 621, 1246, 1309
BssECI CCNNGG 2 cut(s) 666, 884
BssMI GATC 7 cut(s) 448, 555, 630, 657, 709, 1042, 1299
BssNI GRCGYC 1 cut(s) 880
BssT1I CCWWGG 1 cut(s) 666
Bst2UI CCWGG 1 cut(s) 885
Bst4CI ACNGT 2 cut(s) 373, 843
BstACI GRCGYC 1 cut(s) 880
BstAFI CTTAAG 1 cut(s) 364
BstAUI TGTACA 2 cut(s) 373, 700
BstBI TTCGAA 1 cut(s) 107
BstC8I GCNNGC 3 cut(s) 72, 675, 1149
BstDEI CTNAG 4 cut(s) 20, 351, 387, 560
BstDSI CCRYGG 1 cut(s) 666
BstF5I GGATG 6 cut(s) 52, 199, 241, 271, 466, 643
BstFNI CGCG 1 cut(s) 1047
BstHHI GCGC 1 cut(s) 16
BstKTI GATC 7 cut(s) 451, 558, 633, 660, 712, 1045, 1302
BstMAI GTCTC 1 cut(s) 468
BstMBI GATC 7 cut(s) 448, 555, 630, 657, 709, 1042, 1299
BstMCI CGRYCG 1 cut(s) 298
BstMWI GCNNNNNNNGC 1 cut(s) 1283
BstNI CCWGG 1 cut(s) 885
BstNSI RCATGY 1 cut(s) 693
BstSCI CCNGG 1 cut(s) 883
BstSFI CTRYAG 1 cut(s) 714
BstSLI GKGCMC 2 cut(s) 1279, 1309
BstUI CGCG 1 cut(s) 1047
BstV1I GCAGC 2 cut(s) 28, 466
BstX2I RGATCY 2 cut(s) 555, 1299
BstYI RGATCY 2 cut(s) 555, 1299
BsuI GTATCC 2 cut(s) 635, 1158
BsuRI GGCC 2 cut(s) 408, 1012
BtgI CCRYGG 1 cut(s) 666
BtsCI GGATG 6 cut(s) 52, 199, 241, 271, 466, 643
BtsI GCAGTG 1 cut(s) 867
BtsIMutI CAGTG 6 cut(s) 74, 340, 369, 442, 614, 867
BveI ACCTGC 1 cut(s) 297
Cac8I GCNNGC 3 cut(s) 72, 675, 1149
CciI TCATGA 2 cut(s) 216, 976
CfoI GCGC 1 cut(s) 16
Csp6I GTAC 3 cut(s) 200, 374, 701
CviQI GTAC 3 cut(s) 200, 374, 701
DdeI CTNAG 4 cut(s) 20, 351, 387, 560
DpnI GATC 7 cut(s) 450, 557, 632, 659, 711, 1044, 1301
DpnII GATC 7 cut(s) 448, 555, 630, 657, 709, 1042, 1299
DraIII CACNNNGTG 1 cut(s) 1073
Ecl136II GAGCTC 1 cut(s) 355
Eco130I CCWWGG 1 cut(s) 666
Eco24I GRGCYC 1 cut(s) 357
Eco53kI GAGCTC 1 cut(s) 355
Eco57I CTGAAG 3 cut(s) 819, 927, 1308
Eco88I CYCGRG 2 cut(s) 326, 1125
EcoICRI GAGCTC 1 cut(s) 355
EcoRI GAATTC 2 cut(s) 1055, 1339
EcoRII CCWGG 1 cut(s) 883
EcoT14I CCWWGG 1 cut(s) 666
EcoT22I ATGCAT 2 cut(s) 241, 271
EcoT38I GRGCYC 1 cut(s) 357
ErhI CCWWGG 1 cut(s) 666
FalI AAGNNNNNCTT 2 cut(s) 1373, 1405
FaqI GGGAC 1 cut(s) 158
FblI GTMKAC 1 cut(s) 5
Fnu4HI GCNGC 2 cut(s) 17, 480
FokI GGATG 6 cut(s) 39, 206, 248, 278, 453, 650
FriOI GRGCYC 1 cut(s) 357
Fsp4HI GCNGC 2 cut(s) 17, 480
FspBI CTAG 6 cut(s) 516, 582, 605, 684, 950, 1131
FspI TGCGCA 1 cut(s) 15
GlaI GCGC 1 cut(s) 15
GluI GCNGC 2 cut(s) 17, 480
GsaI CCCAGC 1 cut(s) 193
GsuI CTGGAG 1 cut(s) 1266
HaeIII GGCC 2 cut(s) 408, 1012
HapII CCGG 1 cut(s) 257
HhaI GCGC 1 cut(s) 16
Hin1I GRCGYC 1 cut(s) 880
Hin6I GCGC 1 cut(s) 14
HinP1I GCGC 1 cut(s) 14
HincII GTYRAC 3 cut(s) 6, 771, 1399
HindII GTYRAC 3 cut(s) 6, 771, 1399
HinfI GANTC 9 cut(s) 7, 59, 79, 166, 329, 865, 944, 989, 1428
HpaI GTTAAC 1 cut(s) 1399
HpaII CCGG 1 cut(s) 257
HphI GGTGA 5 cut(s) 443, 817, 905, 1202, 1280
Hpy166II GTNNAC 4 cut(s) 6, 701, 771, 1399
Hpy188I TCNGA 6 cut(s) 156, 352, 765, 825, 838, 994
Hpy188III TCNNGA 7 cut(s) 56, 217, 559, 605, 628, 977, 1422
Hpy8I GTNNAC 4 cut(s) 6, 701, 771, 1399
Hpy99I CGWCG 1 cut(s) 882
HpyAV CCTTC 6 cut(s) 179, 256, 823, 1181, 1221, 1309
HpyCH4III ACNGT 2 cut(s) 373, 843
HpyCH4IV ACGT 1 cut(s) 880
HpyF10VI GCNNNNNNNGC 1 cut(s) 1283
HpyF3I CTNAG 4 cut(s) 20, 351, 387, 560
HpySE526I ACGT 1 cut(s) 880
Hsp92I GRCGYC 1 cut(s) 880
HspAI GCGC 1 cut(s) 14
KspAI GTTAAC 1 cut(s) 1399
Kzo9I GATC 7 cut(s) 448, 555, 630, 657, 709, 1042, 1299
LmnI GCTCC 4 cut(s) 360, 670, 736, 796
Lsp1109I GCAGC 2 cut(s) 28, 466
LweI GCATC 4 cut(s) 226, 256, 297, 900
MaeI CTAG 6 cut(s) 516, 582, 605, 684, 950, 1131
MaeII ACGT 1 cut(s) 880
MalI GATC 7 cut(s) 450, 557, 632, 659, 711, 1044, 1301
MboI GATC 7 cut(s) 448, 555, 630, 657, 709, 1042, 1299
MboII GAAGA 9 cut(s) 96, 121, 375, 501, 640, 965, 1051, 1316, 1335
MflI RGATCY 2 cut(s) 555, 1299
MhlI GDGCHC 4 cut(s) 76, 357, 1279, 1309
MluCI AATT 9 cut(s) 544, 566, 588, 1055, 1097, 1138, 1194, 1320, 1339
MlyI GAGTC 4 cut(s) 53, 338, 998, 1422
MnlI CCTC 9 cut(s) 124, 398, 831, 1023, 1070, 1134, 1251, 1290, 1418
Mph1103I ATGCAT 2 cut(s) 241, 271
MroXI GAANNNNTTC 1 cut(s) 583
MseI TTAA 7 cut(s) 242, 360, 365, 591, 1197, 1392, 1398
MspA1I CMGCKG 3 cut(s) 19, 677, 1286
MspCI CTTAAG 1 cut(s) 364
MspI CCGG 1 cut(s) 257
MspR9I CCNGG 1 cut(s) 885
Mva1269I GAATGC 2 cut(s) 519, 873
MvaI CCWGG 1 cut(s) 885
MvnI CGCG 1 cut(s) 1047
MwoI GCNNNNNNNGC 1 cut(s) 1283
NcoI CCATGG 1 cut(s) 666
NdeII GATC 7 cut(s) 448, 555, 630, 657, 709, 1042, 1299
NlaIV GGNNCC 3 cut(s) 153, 672, 1278
NsbI TGCGCA 1 cut(s) 15
NsiI ATGCAT 2 cut(s) 241, 271
NspI RCATGY 1 cut(s) 693
NspV TTCGAA 1 cut(s) 107
PaeR7I CTCGAG 2 cut(s) 326, 1125
PagI TCATGA 2 cut(s) 216, 976
PctI GAATGC 2 cut(s) 519, 873
PdmI GAANNNNTTC 1 cut(s) 583
PfeI GAWTC 4 cut(s) 79, 166, 865, 944
PkrI GCNGC 2 cut(s) 18, 481
PleI GAGTC 4 cut(s) 53, 337, 997, 1422
PpsI GAGTC 4 cut(s) 53, 337, 997, 1422
Psp124BI GAGCTC 1 cut(s) 357
Psp6I CCWGG 1 cut(s) 883
PspFI CCCAGC 1 cut(s) 189
PspGI CCWGG 1 cut(s) 883
PspN4I GGNNCC 3 cut(s) 153, 672, 1278
PspXI VCTCGAGB 2 cut(s) 326, 1125
PsuI RGATCY 2 cut(s) 555, 1299
PvuII CAGCTG 3 cut(s) 19, 677, 1286
RsaI GTAC 3 cut(s) 201, 375, 702
RsaNI GTAC 3 cut(s) 200, 374, 701
SacI GAGCTC 1 cut(s) 357
SalI GTCGAC 1 cut(s) 4
SaqAI TTAA 7 cut(s) 242, 360, 365, 591, 1197, 1392, 1398
SatI GCNGC 2 cut(s) 17, 480
Sau3AI GATC 7 cut(s) 448, 555, 630, 657, 709, 1042, 1299
SchI GAGTC 4 cut(s) 53, 338, 998, 1422
ScrFI CCNGG 1 cut(s) 885
SduI GDGCHC 4 cut(s) 76, 357, 1279, 1309
SfaNI GCATC 4 cut(s) 226, 256, 297, 900
SfcI CTRYAG 1 cut(s) 714
Sfr274I CTCGAG 2 cut(s) 326, 1125
SfuI TTCGAA 1 cut(s) 107
SlaI CTCGAG 2 cut(s) 326, 1125
SmlI CTYRAG 5 cut(s) 326, 364, 1088, 1125, 1420
SmoI CTYRAG 5 cut(s) 326, 364, 1088, 1125, 1420
SpeI ACTAGT 1 cut(s) 581
Sse9I AATT 9 cut(s) 544, 566, 588, 1055, 1097, 1138, 1194, 1320, 1339
SsiI CCGC 3 cut(s) 446, 1047, 1221
SspMI CTAG 6 cut(s) 516, 582, 605, 684, 950, 1131
SstI GAGCTC 1 cut(s) 357
StyD4I CCNGG 1 cut(s) 883
StyI CCWWGG 1 cut(s) 666
TaaI ACNGT 2 cut(s) 373, 843
TaiI ACGT 1 cut(s) 883
TaqII GACCGA 1 cut(s) 312
TasI AATT 9 cut(s) 544, 566, 588, 1055, 1097, 1138, 1194, 1320, 1339
TatI WGTACW 2 cut(s) 373, 700
TfiI GAWTC 4 cut(s) 79, 166, 865, 944
Tru1I TTAA 7 cut(s) 242, 360, 365, 591, 1197, 1392, 1398
Tru9I TTAA 7 cut(s) 242, 360, 365, 591, 1197, 1392, 1398
TscAI CASTG 6 cut(s) 81, 340, 376, 442, 621, 867
TseI GCWGC 2 cut(s) 16, 479
TspDTI ATGAA 9 cut(s) 33, 198, 233, 412, 413, 842, 965, 1316, 1373
TspGWI ACGGA 2 cut(s) 330, 609
TspRI CASTG 6 cut(s) 81, 340, 376, 442, 621, 867
Vha464I CTTAAG 1 cut(s) 364
XapI RAATTY 4 cut(s) 544, 1055, 1097, 1339
XbaI TCTAGA 1 cut(s) 604
XceI RCATGY 1 cut(s) 693
XhoI CTCGAG 2 cut(s) 326, 1125
XmiI GTMKAC 1 cut(s) 5
XmnI GAANNNNTTC 1 cut(s) 583
XspI CTAG 6 cut(s) 516, 582, 605, 684, 950, 1131
ZraI GACGTC 1 cut(s) 881
Zsp2I ATGCAT 2 cut(s) 241, 271
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.