Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr13

Orthogroup ID

OG0011232

Physical Location & Seq

Genomic Coordinates Forward (+)

4216757 .. 4219912

Gene Structure 3156 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD13G1060100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1680 bp

ATGGCGTCTCTTCCTTTCACTCACTTTCTCCTCCTACCCAGGTGCCATTCGGTTTGGCCCAATAGCCGGTCGATGAGTTTCGTGCAGCTTCAGGGTCTTAGATTGCGTGGTAAAAAAGGGGGACTTGCAGCTCTCCCTAAAAAGATAAACTCAGAGAACTCAGCTCTGGAGGGTTCCGTTGAAGATGAGGAGGTGGTTGTGAAGAAGAAGACATCTAGGACTCCTAGAAGAACCCGAAAGAAAGCAACATCCGATAATGAGTCTTCGGATGAGGAAACTGTCATTTCTGCATCTAGTGAAGAGACCAAGAAACCGCGTGGAAGAACTCGTAAGAAAGTTGCATCAGCTTCCACAAGCGTGGAGGAAGAACAAACTGAGAAGAAGGTAAGGAAGAGAAGAACTAAGAAGAAGGACGATGCGATTGAGCAGCAGGTTAGTGACGGTGAACTTAGTGAGACTGAGGATTTTACATTCGTCATGGATATGAAGGATGAGAATGAGGAAGACCTAGAATTGAAAATTGATGAGGGAGAGGATATTAGCGAAACTTACGATTGGCCTCCTCTTGTTTGTTGCTTTGGAGCCGCACAACATGCTTTTGTGCTCTCAGGGAGGCCAGCCAACAGACTTGTAGATTATGAAATACATGAAAGAAAGAAAGATGCGTTATGGGCCCCTGAGAAATTTATTAGGTCTCCTGGGGGATCTGCAGGCAGCGTTGCAATTGCTCTTGCAAGCTTGGGAGGCAAGGTTGCTTTCATGGGAAAACTTGGAGATGATCAGTTTGGTCAGGCCATGCTGTATTATATGAATGTCAAAAATGTTCAAACCCGTTCTGTTCGCATGGATACTAAAAGGGCAACTGCAGTATCACAAATGAAGATTGGTAAAAGGGGTCGCATGAAATTGAGTTGTGTCAAACCATGTGCTGAGGATTCTTTAACAAAGTCAGAGATCAACGTTGATGTGCTGAAGGAGGCAAAGATGTTTTACTTCAGCACAAATTCTCTGCTTGATCGAAACATGAGGTCAACAACACTGCAGGCAATCAAGATTTCAAAGAAACTTGGAGGAGTTATTTTCTACGATGTAAACCTTCCATTGCCACTATGGCAGTCTTGTGAAGAAACAAAGTTGTTCGTACAGCAAGTGTGGAATCTTGCTGATATTATTGAAGTTACTAAGCAAGAGCTTGAGTTTCTCTGCGGGATCCAGCCCTCTGAGGAATTTGACACTAAAAATAATGACAGGTCAAAGTTTGTCCATCATTCACCAGAAGTGGTTGAACAACTTTGGCATGACAATCTTAAGGTTTTGTTCGTGACAAATGGGACTTCGAAGATACACTACTACACAAAGGACCACCAAGGTGCTGTTAACGGAATGGAAGATCCCCCCATCAGTCCTTTCACTTCTGATATGTCAGCATCTGGAGACGGCATTGTGGCAGCTCTCATGAGAATGTTGACAGTTCAACCACATCTTATTACTGATAAAGAATATCTGGAGCACTCAATCAACTATGCAATCGATTGCGGAGTGATAGATCAATGGCTGCTTGGACAAGAACGCGGCTTCCCTCCGAAGGAAGACATGGAAGAGGTGGTTCCTGATTCAGACGGTATAAGGTCGATAACAGAAATGGAATATCGCACTCTGACACCAGAGACTGTAAGTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000427 GO:0000428 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006139 GO:0006351 GO:0006355 GO:0006725 GO:0006807 GO:0006996 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009295 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009657 GO:0009658 GO:0009662 GO:0009889 GO:0009987 GO:0010467 GO:0010468 GO:0010556 GO:0016043 GO:0016070 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0030880 GO:0031323 GO:0031326 GO:0032774 GO:0032991 GO:0034641 GO:0034645 GO:0034654 GO:0042644 GO:0042646 GO:0042793 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046483 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0061695 GO:0065007 GO:0071704 GO:0071840 GO:0080090 GO:0090304 GO:0097659 GO:1901360 GO:1901362 GO:1901576 GO:1902494 GO:1903506 GO:1990234 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PfkB

Protein Analysis

560

Amino Acids

62.96

Weight (kDa)

5.83

Isoelectric Point (pI)

50.41

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PfkB	PF00294	220 - 491	1.2e-30	pfkB family carbohydrate kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0011232)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G69200
fragaria_vesca	FvH4_4g29720 FvH4_4g29720 FvH4_4g29720 FvH4_4g29720
malus_domestica	MD13G1060100.v1.1
prunus_persica	Prupe.1G289300_v2.0.a1 Prupe.1G289300_v2.0.a1
pyrus_communis	pycom13g05350
rosa_chinensis	RchiOBHm_Chr4g0437971
rosa_laevigata	RLG00000006397
rosa_multiflora	Rmu_sc0007538.1_g000008
rosa_roxburghii	Rroxscaffold_5G00379130
rosa_rugosa	Rorug04G0306000
rosa_samantha	Rh4AG357600 Rh4BG368500 Rh4CG382600 Rh4DG363400
rosa_wichuraiana	Rw0G011510 Rw0G016160

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	421
AccB1I	GGYRCC	1 cut(s)	42
AccII	CGCG	2 cut(s)	316, 1572
AciI	CCGC	5 cut(s)	314, 585, 1206, 1536, 1572
AclI	AACGTT	1 cut(s)	960
AclWI	GGATC	4 cut(s)	712, 1204, 1217, 1385
AcsI	RAATTY	3 cut(s)	683, 1003, 1226
AcuI	CTGAAG	3 cut(s)	74, 979, 992
AcyI	GRCGYC	1 cut(s)	5
AfaI	GTAC	1 cut(s)	1143
AfiI	CCNNNNNNNGG	2 cut(s)	66, 1585
AflII	CTTAAG	1 cut(s)	1307
AgsI	TTSAA	7 cut(s)	182, 517, 827, 1059, 1175, 1286, 1475
AjnI	CCWGG	2 cut(s)	38, 697
AleI	CACNNNNGTG	2 cut(s)	356, 1368
AloI	GAACNNNNNNTCC	2 cut(s)	1560, 1592
AluBI	AGCT	7 cut(s)	88, 131, 164, 347, 738, 1192, 1451
AluI	AGCT	7 cut(s)	88, 131, 164, 347, 738, 1192, 1451
Alw21I	GWGCWC	2 cut(s)	606, 1512
Alw26I	GTCTC	6 cut(s)	12, 296, 449, 699, 1428, 1661
AlwI	GGATC	4 cut(s)	712, 1204, 1217, 1385
AlwNI	CAGNNNCTG	2 cut(s)	1430, 1670
AoxI	GGCC	5 cut(s)	56, 557, 614, 672, 792
ApaI	GGGCCC	1 cut(s)	676
ApeKI	GCWGC	6 cut(s)	85, 128, 427, 714, 1448, 1555
ApoI	RAATTY	3 cut(s)	683, 1003, 1226
AspS9I	GGNCC	4 cut(s)	57, 672, 673, 1360
AsuHPI	GGTGA	2 cut(s)	455, 1263
AsuII	TTCGAA	1 cut(s)	1337
AvaII	GGWCC	1 cut(s)	1360
BaeGI	GKGCMC	1 cut(s)	676
BaeI	ACNNNNGTAYC	2 cut(s)	852, 885
BamHI	GGATCC	1 cut(s)	1209
BanI	GGYRCC	1 cut(s)	42
BanII	GRGCYC	1 cut(s)	676
BarI	GAAGNNNNNNTAC	2 cut(s)	1331, 1363
BbsI	GAAGAC	4 cut(s)	215, 255, 510, 1596
Bbv12I	GWGCWC	2 cut(s)	606, 1512
BbvCI	CCTCAGC	1 cut(s)	930
BbvI	GCAGC	6 cut(s)	97, 140, 439, 726, 1460, 1542
BccI	CCATC	2 cut(s)	1272, 1406
BceAI	ACGGC	1 cut(s)	1453
BciT130I	CCWGG	2 cut(s)	40, 699
BciVI	GTATCC	1 cut(s)	841
BclI	TGATCA	1 cut(s)	778
BcoDI	GTCTC	6 cut(s)	12, 296, 449, 699, 1428, 1661
BfaI	CTAG	4 cut(s)	216, 225, 294, 509
BfmI	CTRYAG	3 cut(s)	708, 864, 1040
BfrI	CTTAAG	1 cut(s)	1307
BfuAI	ACCTGC	1 cut(s)	421
BfuI	GTATCC	1 cut(s)	841
BglI	GCCNNNNNGGC	1 cut(s)	1111
BisI	GCNGC	8 cut(s)	86, 129, 428, 585, 715, 1449, 1556, 1573
BlsI	GCNGC	8 cut(s)	87, 130, 429, 586, 716, 1450, 1557, 1574
Bme1390I	CCNGG	2 cut(s)	40, 699
Bme18I	GGWCC	1 cut(s)	1360
BmgT120I	GGNCC	4 cut(s)	57, 672, 673, 1360
BmiI	GGNNCC	7 cut(s)	44, 175, 583, 674, 675, 1211, 1608
BmrFI	CCNGG	2 cut(s)	40, 699
BmsI	GCATC	5 cut(s)	299, 350, 406, 652, 1436
BpiI	GAAGAC	4 cut(s)	215, 255, 510, 1596
BpmI	CTGGAG	3 cut(s)	188, 1452, 1526
Bpu10I	CCTNAGC	1 cut(s)	930
Bpu14I	TTCGAA	1 cut(s)	1337
BpuEI	CTTGAG	1 cut(s)	1214
Bsa29I	ATCGAT	1 cut(s)	1530
BsaHI	GRCGYC	1 cut(s)	5
BsaI	GGTCTC	2 cut(s)	296, 699
BsaJI	CCNNGG	3 cut(s)	38, 698, 1366
BsaXI	ACNNNNNCTCC	2 cut(s)	735, 765
Bsc4I	CCNNNNNNNGG	2 cut(s)	66, 1585
Bse118I	RCCGGY	1 cut(s)	66
Bse3DI	GCAATG	1 cut(s)	1100
BseBI	CCWGG	2 cut(s)	40, 699
BseCI	ATCGAT	1 cut(s)	1530
BseDI	CCNNGG	3 cut(s)	38, 698, 1366
BseGI	GGATG	3 cut(s)	248, 274, 496
BseLI	CCNNNNNNNGG	2 cut(s)	66, 1585
BseMI	GCAATG	1 cut(s)	1100
BseMII	CTCAG	8 cut(s)	165, 174, 366, 450, 621, 669, 921, 1212
BseRI	GAGGAG	4 cut(s)	20, 203, 552, 1086
BseSI	GKGCMC	1 cut(s)	676
BseXI	GCAGC	6 cut(s)	97, 140, 439, 726, 1460, 1542
BsgI	GTGCAG	1 cut(s)	104
Bsh1236I	CGCG	2 cut(s)	316, 1572
Bsh1285I	CGRYCG	1 cut(s)	71
BshFI	GGCC	5 cut(s)	58, 559, 616, 674, 794
BshNI	GGYRCC	1 cut(s)	42
BshVI	ATCGAT	1 cut(s)	1530
BsiEI	CGRYCG	1 cut(s)	71
BsiHKAI	GWGCWC	2 cut(s)	606, 1512
BsiSI	CCGG	1 cut(s)	67
BslFI	GGGAC	2 cut(s)	135, 1345
BslI	CCNNNNNNNGG	2 cut(s)	66, 1585
BsmAI	GTCTC	6 cut(s)	12, 296, 449, 699, 1428, 1661
BsmBI	CGTCTC	2 cut(s)	12, 1428
BsmFI	GGGAC	2 cut(s)	135, 1345
BsnI	GGCC	5 cut(s)	58, 559, 616, 674, 794
Bso31I	GGTCTC	2 cut(s)	296, 699
Bsp119I	TTCGAA	1 cut(s)	1337
Bsp120I	GGGCCC	1 cut(s)	672
Bsp1286I	GDGCHC	3 cut(s)	606, 676, 1512
Bsp143I	GATC	7 cut(s)	704, 778, 954, 1015, 1209, 1390, 1546
BspACI	CCGC	5 cut(s)	314, 585, 1206, 1536, 1572
BspANI	GGCC	5 cut(s)	58, 559, 616, 674, 794
BspCNI	CTCAG	8 cut(s)	164, 173, 367, 451, 620, 670, 922, 1213
BspDI	ATCGAT	1 cut(s)	1530
BspFNI	CGCG	2 cut(s)	316, 1572
BspHI	TCATGA	1 cut(s)	1455
BspLI	GGNNCC	7 cut(s)	44, 175, 583, 674, 675, 1211, 1608
BspMAI	CTGCAG	3 cut(s)	712, 868, 1044
BspMI	ACCTGC	1 cut(s)	421
BspPI	GGATC	4 cut(s)	712, 1204, 1217, 1385
BspT104I	TTCGAA	1 cut(s)	1337
BspT107I	GGYRCC	1 cut(s)	42
BspTI	CTTAAG	1 cut(s)	1307
BspTNI	GGTCTC	2 cut(s)	296, 699
BsrDI	GCAATG	1 cut(s)	1100
BsrFI	RCCGGY	1 cut(s)	66
BssAI	RCCGGY	1 cut(s)	66
BssECI	CCNNGG	3 cut(s)	38, 698, 1366
BssMI	GATC	7 cut(s)	704, 778, 954, 1015, 1209, 1390, 1546
BssNI	GRCGYC	1 cut(s)	5
BssT1I	CCWWGG	1 cut(s)	1366
Bst2UI	CCWGG	2 cut(s)	40, 699
Bst4CI	ACNGT	5 cut(s)	280, 443, 1471, 1622, 1672
Bst6I	CTCTTC	4 cut(s)	15, 294, 386, 1593
BstACI	GRCGYC	1 cut(s)	5
BstAFI	CTTAAG	1 cut(s)	1307
BstAPI	GCANNNNNTGC	1 cut(s)	593
BstBI	TTCGAA	1 cut(s)	1337
BstC8I	GCNNGC	4 cut(s)	618, 712, 736, 1044
BstF5I	GGATG	3 cut(s)	248, 274, 496
BstFNI	CGCG	2 cut(s)	316, 1572
BstKTI	GATC	7 cut(s)	707, 781, 957, 1018, 1212, 1393, 1549
BstMAI	GTCTC	6 cut(s)	12, 296, 449, 699, 1428, 1661
BstMBI	GATC	7 cut(s)	704, 778, 954, 1015, 1209, 1390, 1546
BstMCI	CGRYCG	1 cut(s)	71
BstMWI	GCNNNNNNNGC	4 cut(s)	593, 671, 744, 1111
BstNI	CCWGG	2 cut(s)	40, 699
BstNSI	RCATGY	1 cut(s)	596
BstSCI	CCNGG	2 cut(s)	38, 697
BstSFI	CTRYAG	3 cut(s)	708, 864, 1040
BstSLI	GKGCMC	1 cut(s)	676
BstUI	CGCG	2 cut(s)	316, 1572
BstV1I	GCAGC	6 cut(s)	97, 140, 439, 726, 1460, 1542
BstV2I	GAAGAC	4 cut(s)	215, 255, 510, 1596
BstX2I	RGATCY	3 cut(s)	704, 1209, 1390
BstXI	CCANNNNNNTGG	1 cut(s)	358
BstYI	RGATCY	3 cut(s)	704, 1209, 1390
Bsu15I	ATCGAT	1 cut(s)	1530
BsuI	GTATCC	1 cut(s)	841
BsuRI	GGCC	5 cut(s)	58, 559, 616, 674, 794
BsuTUI	ATCGAT	1 cut(s)	1530
BtsCI	GGATG	3 cut(s)	248, 274, 496
BtsI	GCAGTG	1 cut(s)	1037
BtsIMutI	CAGTG	1 cut(s)	1037
BveI	ACCTGC	1 cut(s)	421
Cac8I	GCNNGC	4 cut(s)	618, 712, 736, 1044
CaiI	CAGNNNCTG	2 cut(s)	1430, 1670
CciI	TCATGA	1 cut(s)	1455
Cfr10I	RCCGGY	1 cut(s)	66
Cfr13I	GGNCC	4 cut(s)	57, 672, 673, 1360
ClaI	ATCGAT	1 cut(s)	1530
Csp6I	GTAC	1 cut(s)	1142
CviQI	GTAC	1 cut(s)	1142
DpnI	GATC	7 cut(s)	706, 780, 956, 1017, 1211, 1392, 1548
DpnII	GATC	7 cut(s)	704, 778, 954, 1015, 1209, 1390, 1546
Eam1104I	CTCTTC	4 cut(s)	15, 294, 386, 1593
EarI	CTCTTC	4 cut(s)	15, 294, 386, 1593
Eco130I	CCWWGG	1 cut(s)	1366
Eco24I	GRGCYC	1 cut(s)	676
Eco31I	GGTCTC	2 cut(s)	296, 699
Eco47I	GGWCC	1 cut(s)	1360
Eco57I	CTGAAG	3 cut(s)	74, 979, 992
EcoO109I	RGGNCCY	1 cut(s)	673
EcoRII	CCWGG	2 cut(s)	38, 697
EcoT14I	CCWWGG	1 cut(s)	1366
EcoT38I	GRGCYC	1 cut(s)	676
ErhI	CCWWGG	1 cut(s)	1366
Esp3I	CGTCTC	2 cut(s)	12, 1428
FalI	AAGNNNNNCTT	2 cut(s)	108, 140
FaqI	GGGAC	2 cut(s)	135, 1345
FauI	CCCGC	1 cut(s)	1199
FbaI	TGATCA	1 cut(s)	778
Fnu4HI	GCNGC	8 cut(s)	86, 129, 428, 585, 715, 1449, 1556, 1573
FokI	GGATG	3 cut(s)	235, 281, 503
FriOI	GRGCYC	1 cut(s)	676
Fsp4HI	GCNGC	8 cut(s)	86, 129, 428, 585, 715, 1449, 1556, 1573
FspBI	CTAG	4 cut(s)	216, 225, 294, 509
GluI	GCNGC	8 cut(s)	86, 129, 428, 585, 715, 1449, 1556, 1573
GsuI	CTGGAG	3 cut(s)	188, 1452, 1526
HaeIII	GGCC	5 cut(s)	58, 559, 616, 674, 794
HapII	CCGG	1 cut(s)	67
Hin1I	GRCGYC	1 cut(s)	5
HincII	GTYRAC	3 cut(s)	1032, 1378, 1467
HindII	GTYRAC	3 cut(s)	1032, 1378, 1467
HindIII	AAGCTT	1 cut(s)	736
HinfI	GANTC	5 cut(s)	220, 260, 935, 1156, 1613
HpaI	GTTAAC	1 cut(s)	1378
HpaII	CCGG	1 cut(s)	67
HphI	GGTGA	2 cut(s)	455, 1263
Hpy166II	GTNNAC	5 cut(s)	446, 1032, 1093, 1378, 1467
Hpy188I	TCNGA	9 cut(s)	154, 253, 268, 952, 1222, 1417, 1584, 1618, 1659
Hpy188III	TCNNGA	7 cut(s)	167, 1051, 1321, 1431, 1456, 1505, 1610
Hpy8I	GTNNAC	5 cut(s)	446, 1032, 1093, 1378, 1467
HpyAV	CCTTC	6 cut(s)	376, 403, 481, 967, 1106, 1579
HpyCH4III	ACNGT	5 cut(s)	280, 443, 1471, 1622, 1672
HpyCH4IV	ACGT	1 cut(s)	960
HpyF10VI	GCNNNNNNNGC	4 cut(s)	593, 671, 744, 1111
HpySE526I	ACGT	1 cut(s)	960
Hsp92I	GRCGYC	1 cut(s)	5
Ksp22I	TGATCA	1 cut(s)	778
KspAI	GTTAAC	1 cut(s)	1378
Kzo9I	GATC	7 cut(s)	704, 778, 954, 1015, 1209, 1390, 1546
LmnI	GCTCC	2 cut(s)	581, 1507
Lsp1109I	GCAGC	6 cut(s)	97, 140, 439, 726, 1460, 1542
LweI	GCATC	5 cut(s)	299, 350, 406, 652, 1436
MaeI	CTAG	4 cut(s)	216, 225, 294, 509
MaeII	ACGT	1 cut(s)	960
MaeIII	GTNAC	3 cut(s)	437, 1177, 1321
MalI	GATC	7 cut(s)	706, 780, 956, 1017, 1211, 1392, 1548
MboI	GATC	7 cut(s)	704, 778, 954, 1015, 1209, 1390, 1546
MfeI	CAATTG	1 cut(s)	723
MflI	RGATCY	3 cut(s)	704, 1209, 1390
MhlI	GDGCHC	3 cut(s)	606, 676, 1512
MluCI	AATT	7 cut(s)	512, 519, 683, 723, 905, 1003, 1226
MlyI	GAGTC	2 cut(s)	214, 269
MseI	TTAA	3 cut(s)	941, 1308, 1377
MslI	CAYNNNNRTG	4 cut(s)	356, 482, 1368, 1460
MspCI	CTTAAG	1 cut(s)	1307
MspI	CCGG	1 cut(s)	67
MspR9I	CCNGG	2 cut(s)	40, 699
MunI	CAATTG	1 cut(s)	723
MvaI	CCWGG	2 cut(s)	40, 699
MvnI	CGCG	2 cut(s)	316, 1572
MwoI	GCNNNNNNNGC	4 cut(s)	593, 671, 744, 1111
NdeII	GATC	7 cut(s)	704, 778, 954, 1015, 1209, 1390, 1546
NlaIV	GGNNCC	7 cut(s)	44, 175, 583, 674, 675, 1211, 1608
NmuCI	GTSAC	2 cut(s)	437, 1321
NspI	RCATGY	1 cut(s)	596
NspV	TTCGAA	1 cut(s)	1337
OliI	CACNNNNGTG	2 cut(s)	356, 1368
PagI	TCATGA	1 cut(s)	1455
PfeI	GAWTC	3 cut(s)	935, 1156, 1613
PkrI	GCNGC	8 cut(s)	87, 130, 429, 586, 716, 1450, 1557, 1574
PleI	GAGTC	2 cut(s)	214, 268
PpsI	GAGTC	2 cut(s)	214, 268
Psp1406I	AACGTT	1 cut(s)	960
Psp6I	CCWGG	2 cut(s)	38, 697
PspGI	CCWGG	2 cut(s)	38, 697
PspN4I	GGNNCC	7 cut(s)	44, 175, 583, 674, 675, 1211, 1608
PspOMI	GGGCCC	1 cut(s)	672
PspPI	GGNCC	4 cut(s)	57, 672, 673, 1360
PstI	CTGCAG	3 cut(s)	712, 868, 1044
PstNI	CAGNNNCTG	2 cut(s)	1430, 1670
PsuI	RGATCY	3 cut(s)	704, 1209, 1390
RsaI	GTAC	1 cut(s)	1143
RsaNI	GTAC	1 cut(s)	1142
RseI	CAYNNNNRTG	4 cut(s)	356, 482, 1368, 1460
SaqAI	TTAA	3 cut(s)	941, 1308, 1377
SatI	GCNGC	8 cut(s)	86, 129, 428, 585, 715, 1449, 1556, 1573
Sau3AI	GATC	7 cut(s)	704, 778, 954, 1015, 1209, 1390, 1546
Sau96I	GGNCC	4 cut(s)	57, 672, 673, 1360
SchI	GAGTC	2 cut(s)	214, 269
ScrFI	CCNGG	2 cut(s)	40, 699
SduI	GDGCHC	3 cut(s)	606, 676, 1512
SfaNI	GCATC	5 cut(s)	299, 350, 406, 652, 1436
SfcI	CTRYAG	3 cut(s)	708, 864, 1040
SfuI	TTCGAA	1 cut(s)	1337
SinI	GGWCC	1 cut(s)	1360
SmiMI	CAYNNNNRTG	4 cut(s)	356, 482, 1368, 1460
SmlI	CTYRAG	2 cut(s)	1193, 1307
SmoI	CTYRAG	2 cut(s)	1193, 1307
Sse9I	AATT	7 cut(s)	512, 519, 683, 723, 905, 1003, 1226
SsiI	CCGC	5 cut(s)	314, 585, 1206, 1536, 1572
SspMI	CTAG	4 cut(s)	216, 225, 294, 509
StyD4I	CCNGG	2 cut(s)	38, 697
StyI	CCWWGG	1 cut(s)	1366
TaaI	ACNGT	5 cut(s)	280, 443, 1471, 1622, 1672
TaiI	ACGT	1 cut(s)	963
TaqI	TCGA	5 cut(s)	71, 1018, 1337, 1530, 1631
TasI	AATT	7 cut(s)	512, 519, 683, 723, 905, 1003, 1226
TauI	GCSGC	2 cut(s)	587, 1575
TfiI	GAWTC	3 cut(s)	935, 1156, 1613
Tru1I	TTAA	3 cut(s)	941, 1308, 1377
Tru9I	TTAA	3 cut(s)	941, 1308, 1377
TscAI	CASTG	1 cut(s)	1044
TseFI	GTSAC	2 cut(s)	437, 1321
TseI	GCWGC	6 cut(s)	85, 128, 427, 714, 1448, 1555
Tsp45I	GTSAC	2 cut(s)	437, 1321
TspDTI	ATGAA	7 cut(s)	500, 654, 663, 748, 824, 893, 917
TspGWI	ACGGA	2 cut(s)	166, 1395
TspRI	CASTG	1 cut(s)	1044
Vha464I	CTTAAG	1 cut(s)	1307
VpaK11BI	GGWCC	1 cut(s)	1360
XapI	RAATTY	3 cut(s)	683, 1003, 1226
XceI	RCATGY	1 cut(s)	596
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1107
XspI	CTAG	4 cut(s)	216, 225, 294, 509

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.