Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr4C

Orthogroup ID

OG0011232

Physical Location & Seq

Genomic Coordinates Forward (+)

63586145 .. 63589721

Gene Structure 3577 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh4CG382600.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1449 bp

ATGGCGTCGCTCTCTTTCACACACTTTCTACAATTACCCAGGTGCCATTTGGATTGGCCCAATAAGCCGTCACTTAATTTAGTGCAACTTCAGGGTCTTAGATTACACAGTAAAAAATGGGGACTTGCTGCAATGTCTAAAAAGATAAACAATTCGGAGAATTCAGCTCTAGATGCAATGAATGAAGAGGAGGTTGTGGTGAAGAAGAAAACATCTAGGACTTCTAAACGTACTCGGAAGAAGACAGCATCAGATAGTGATACTTCGAGTGATGAAGATGTCGGTGAAGAGCCCAAGAAAACCCGGGGAAGACGTAGGAAAGCTGCATCTGCTTCGGTAAGTGTGGAGGGAGAACAAACTGAGAAGAAGGTGAGGAAGAGAAGAACTAAGAAGAAGGTTGATGAGATTGAGCATCAGGTTAGTGAAGCTGAAGTTAATGAGGCTGAGGAGTTTACATTTGTTTTGGATGTGAAGGATGAGAGTGAGGATGATCTAGAATTGGAAATAGATGACGGGGAGGATATCAGCTGTACTTATGGTTGGCCTCCTCTTGTCTGTTGCTTCGGAGCTGCTCAACATGCTTTTGTGCCATCGGGTAGGCCAGCCAACAGACTCTTAGATTATGAAATACATGAAAGAAAGAAAGATGCATTATGGGCCCCTGAAAAGTTCGTTAGGTCTCCTGGGGGATCTGCAGGCAGTGTAGCGATTACTCTTGCAAAATTGGGCGGCAAGGTTGCTTTCATGGGAAAACTTGGGGATGATGACTACGGCAAGGCCATGTTATATTATATGAATGCCAACAATGTTCAAACCCGGTCTGTTCGCATGGATAGTAGAAGAGCAACTGCAGTATCACAGATGAAGATCGGTAAAAGAAGTCGCTTGAGATTGACTTGTGTCAAACCCTGTGCTGAGGATTCTTTATCAAAGTCGGATATCAACATGGATGTGCTGAAGGAGGCAAAGATGTTCTACTTCAACACACATTCTCTGCTTGATCAAAACATGAGATCAACTACACTCCAAGCAATCAAAATTTCAAAGAAGCTAGGTGGAGTTGTTTTCTATGATGTAAACCTTCCATTACCGTTATGGCAATCATCTGAAGAAACCAAGTTGTTCATACAGCAAGTATGGAATCTTTCTGATATTATTGAAGTTACTAAGCAAGAGCTTGAGTTTCTATGTGGAGTCCAGCCTTCTGAGGAATTCGATACCAAAAATAATGCCAGATCGAAGTTTGTCCATTATACACAAGAAATGATTGCACCACTTTGGCATGAAAATCTTAAGGTTTTGTTTGTGACAAATGGGACTTCTAAGATACATTACTATACAAAGGAGCATGATGGTGCTGTTAATGGGATGGAGGATCCCCCTATTAGTCCTTTCACTTCTGATATGTCAGCATCTGGAGATGGTATTGTGGCAGGTACACTCTTGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000427 GO:0000428 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006139 GO:0006351 GO:0006355 GO:0006725 GO:0006807 GO:0006996 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009295 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009657 GO:0009658 GO:0009662 GO:0009889 GO:0009987 GO:0010467 GO:0010468 GO:0010556 GO:0016043 GO:0016070 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0030880 GO:0031323 GO:0031326 GO:0032774 GO:0032991 GO:0034641 GO:0034645 GO:0034654 GO:0042644 GO:0042646 GO:0042793 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046483 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0061695 GO:0065007 GO:0071704 GO:0071840 GO:0080090 GO:0090304 GO:0097659 GO:1901360 GO:1901362 GO:1901576 GO:1902494 GO:1903506 GO:1990234 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PfkB

Protein Analysis

482

Amino Acids

54.14

Weight (kDa)

7.16

Isoelectric Point (pI)

54.71

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PfkB	PF00294	214 - 479	1.9e-28	pfkB family carbohydrate kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0011232)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G69200
fragaria_vesca	FvH4_4g29720 FvH4_4g29720 FvH4_4g29720 FvH4_4g29720
malus_domestica	MD13G1060100.v1.1
prunus_persica	Prupe.1G289300_v2.0.a1 Prupe.1G289300_v2.0.a1
pyrus_communis	pycom13g05350
rosa_chinensis	RchiOBHm_Chr4g0437971
rosa_laevigata	RLG00000006397
rosa_multiflora	Rmu_sc0007538.1_g000008
rosa_roxburghii	Rroxscaffold_5G00379130
rosa_rugosa	Rorug04G0306000
rosa_samantha	Rh4AG357600 Rh4BG368500 Rh4CG382600 Rh4DG363400
rosa_wichuraiana	Rw0G011510 Rw0G016160

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	1424
AccB1I	GGYRCC	1 cut(s)	42
AciI	CCGC	1 cut(s)	729
AclWI	GGATC	3 cut(s)	697, 1370, 1383
AcsI	RAATTY	3 cut(s)	160, 1038, 1211
AcuI	CTGAAG	4 cut(s)	74, 450, 977, 1128
AcyI	GRCGYC	1 cut(s)	5
AfaI	GTAC	3 cut(s)	232, 532, 1438
AflII	CTTAAG	1 cut(s)	1292
AgsI	TTSAA	4 cut(s)	812, 982, 1044, 1160
AjnI	CCWGG	2 cut(s)	38, 682
AleI	CACNNNNGTG	1 cut(s)	1444
AluBI	AGCT	7 cut(s)	167, 323, 428, 528, 569, 1051, 1177
AluI	AGCT	7 cut(s)	167, 323, 428, 528, 569, 1051, 1177
Alw26I	GTCTC	1 cut(s)	684
AlwI	GGATC	3 cut(s)	697, 1370, 1383
AlwNI	CAGNNNCTG	1 cut(s)	1415
Ama87I	CYCGRG	1 cut(s)	303
AoxI	GGCC	5 cut(s)	56, 542, 599, 657, 777
ApaI	GGGCCC	1 cut(s)	661
ApeKI	GCWGC	3 cut(s)	128, 323, 569
ApoI	RAATTY	3 cut(s)	160, 1038, 1211
AspS9I	GGNCC	3 cut(s)	57, 657, 658
AsuC2I	CCSGG	3 cut(s)	304, 305, 817
AsuHPI	GGTGA	3 cut(s)	211, 296, 382
AvaI	CYCGRG	1 cut(s)	303
BaeGI	GKGCMC	1 cut(s)	661
BaeI	ACNNNNGTAYC	2 cut(s)	837, 870
BamHI	GGATCC	1 cut(s)	1375
BanI	GGYRCC	1 cut(s)	42
BanII	GRGCYC	2 cut(s)	294, 661
BbsI	GAAGAC	2 cut(s)	248, 316
BbvCI	CCTCAGC	2 cut(s)	444, 915
BbvI	GCAGC	3 cut(s)	115, 310, 556
BccI	CCATC	4 cut(s)	598, 1346, 1363, 1415
BceAI	ACGGC	2 cut(s)	52, 787
BcgI	CGANNNNNNTGC	2 cut(s)	315, 349
BciT130I	CCWGG	2 cut(s)	40, 684
BclI	TGATCA	1 cut(s)	1000
BcnI	CCSGG	3 cut(s)	304, 305, 817
BcoDI	GTCTC	1 cut(s)	684
BfaI	CTAG	4 cut(s)	170, 216, 494, 1052
BfmI	CTRYAG	2 cut(s)	693, 849
BfrI	CTTAAG	1 cut(s)	1292
BfuAI	ACCTGC	1 cut(s)	1424
BisI	GCNGC	4 cut(s)	129, 324, 570, 730
BlsI	GCNGC	4 cut(s)	130, 325, 571, 731
Bme1390I	CCNGG	5 cut(s)	40, 304, 305, 684, 817
BmeT110I	CYCGRG	1 cut(s)	303
BmgT120I	GGNCC	3 cut(s)	57, 657, 658
BmiI	GGNNCC	4 cut(s)	44, 659, 660, 1377
BmrFI	CCNGG	5 cut(s)	40, 304, 305, 684, 817
BmsI	GCATC	6 cut(s)	163, 257, 335, 421, 637, 1421
BoxI	GACNNNNGTC	1 cut(s)	899
BpiI	GAAGAC	2 cut(s)	248, 316
BpmI	CTGGAG	1 cut(s)	1437
Bpu10I	CCTNAGC	2 cut(s)	444, 915
BpuEI	CTTGAG	2 cut(s)	907, 1199
BpuMI	CCSGG	3 cut(s)	304, 305, 817
BsaBI	GATNNNNATC	1 cut(s)	866
BsaHI	GRCGYC	1 cut(s)	5
BsaI	GGTCTC	1 cut(s)	684
BsaJI	CCNNGG	4 cut(s)	38, 303, 304, 683
Bse3DI	GCAATG	2 cut(s)	138, 183
Bse8I	GATNNNNATC	1 cut(s)	866
BseBI	CCWGG	2 cut(s)	40, 684
BseDI	CCNNGG	4 cut(s)	38, 303, 304, 683
BseGI	GGATG	6 cut(s)	472, 481, 493, 766, 955, 1374
BseJI	GATNNNNATC	1 cut(s)	866
BseMI	GCAATG	2 cut(s)	138, 183
BseMII	CTCAG	4 cut(s)	351, 435, 906, 1197
BseRI	GAGGAG	3 cut(s)	203, 461, 537
BseSI	GKGCMC	1 cut(s)	661
BseXI	GCAGC	3 cut(s)	115, 310, 556
BshFI	GGCC	5 cut(s)	58, 544, 601, 659, 779
BshNI	GGYRCC	1 cut(s)	42
BsiHKCI	CYCGRG	1 cut(s)	303
BsiSI	CCGG	2 cut(s)	304, 817
BslFI	GGGAC	2 cut(s)	135, 1330
BsmAI	GTCTC	1 cut(s)	684
BsmFI	GGGAC	2 cut(s)	135, 1330
BsmI	GAATGC	1 cut(s)	802
BsnI	GGCC	5 cut(s)	58, 544, 601, 659, 779
Bso31I	GGTCTC	1 cut(s)	684
BsoBI	CYCGRG	1 cut(s)	303
Bsp120I	GGGCCC	1 cut(s)	657
Bsp1286I	GDGCHC	2 cut(s)	294, 661
Bsp143I	GATC	7 cut(s)	490, 689, 867, 1000, 1013, 1235, 1375
BspACI	CCGC	1 cut(s)	729
BspANI	GGCC	5 cut(s)	58, 544, 601, 659, 779
BspCNI	CTCAG	4 cut(s)	352, 436, 907, 1198
BspLI	GGNNCC	4 cut(s)	44, 659, 660, 1377
BspMAI	CTGCAG	2 cut(s)	697, 853
BspMI	ACCTGC	1 cut(s)	1424
BspPI	GGATC	3 cut(s)	697, 1370, 1383
BspQI	GCTCTTC	2 cut(s)	282, 835
BspT107I	GGYRCC	1 cut(s)	42
BspTI	CTTAAG	1 cut(s)	1292
BspTNI	GGTCTC	1 cut(s)	684
BsrDI	GCAATG	2 cut(s)	138, 183
BssECI	CCNNGG	4 cut(s)	38, 303, 304, 683
BssMI	GATC	7 cut(s)	490, 689, 867, 1000, 1013, 1235, 1375
BssNI	GRCGYC	1 cut(s)	5
Bst2UI	CCWGG	2 cut(s)	40, 684
Bst4CI	ACNGT	2 cut(s)	110, 1092
Bst6I	CTCTTC	4 cut(s)	180, 282, 371, 835
BstACI	GRCGYC	1 cut(s)	5
BstAFI	CTTAAG	1 cut(s)	1292
BstC8I	GCNNGC	2 cut(s)	603, 697
BstDEI	CTNAG	9 cut(s)	98, 360, 387, 444, 616, 915, 1167, 1206, 1323
BstF5I	GGATG	6 cut(s)	472, 481, 493, 766, 955, 1374
BstKTI	GATC	7 cut(s)	493, 692, 870, 1003, 1016, 1238, 1378
BstMAI	GTCTC	1 cut(s)	684
BstMBI	GATC	7 cut(s)	490, 689, 867, 1000, 1013, 1235, 1375
BstMWI	GCNNNNNNNGC	5 cut(s)	64, 173, 329, 578, 656
BstNI	CCWGG	2 cut(s)	40, 684
BstNSI	RCATGY	1 cut(s)	581
BstPAI	GACNNNNGTC	1 cut(s)	899
BstSCI	CCNGG	5 cut(s)	38, 302, 303, 682, 815
BstSFI	CTRYAG	2 cut(s)	693, 849
BstSLI	GKGCMC	1 cut(s)	661
BstV1I	GCAGC	3 cut(s)	115, 310, 556
BstV2I	GAAGAC	2 cut(s)	248, 316
BstX2I	RGATCY	2 cut(s)	689, 1375
BstYI	RGATCY	2 cut(s)	689, 1375
BsuRI	GGCC	5 cut(s)	58, 544, 601, 659, 779
BtsCI	GGATG	6 cut(s)	472, 481, 493, 766, 955, 1374
BtsI	GCAGTG	1 cut(s)	706
BtsIMutI	CAGTG	1 cut(s)	706
BveI	ACCTGC	1 cut(s)	1424
Cac8I	GCNNGC	2 cut(s)	603, 697
CaiI	CAGNNNCTG	1 cut(s)	1415
Cfr13I	GGNCC	3 cut(s)	57, 657, 658
Cfr9I	CCCGGG	1 cut(s)	303
Csp6I	GTAC	3 cut(s)	231, 531, 1437
CviAII	CATG	9 cut(s)	578, 632, 745, 781, 829, 946, 1009, 1283, 1349
CviQI	GTAC	3 cut(s)	231, 531, 1437
DdeI	CTNAG	9 cut(s)	98, 360, 387, 444, 616, 915, 1167, 1206, 1323
DpnI	GATC	7 cut(s)	492, 691, 869, 1002, 1015, 1237, 1377
DpnII	GATC	7 cut(s)	490, 689, 867, 1000, 1013, 1235, 1375
Eam1104I	CTCTTC	4 cut(s)	180, 282, 371, 835
EarI	CTCTTC	4 cut(s)	180, 282, 371, 835
Eco24I	GRGCYC	2 cut(s)	294, 661
Eco31I	GGTCTC	1 cut(s)	684
Eco32I	GATATC	2 cut(s)	523, 940
Eco57I	CTGAAG	4 cut(s)	74, 450, 977, 1128
Eco88I	CYCGRG	1 cut(s)	303
EcoO109I	RGGNCCY	1 cut(s)	658
EcoRI	GAATTC	2 cut(s)	160, 1211
EcoRII	CCWGG	2 cut(s)	38, 682
EcoRV	GATATC	2 cut(s)	523, 940
EcoT22I	ATGCAT	1 cut(s)	652
EcoT38I	GRGCYC	2 cut(s)	294, 661
FaeI	CATG	9 cut(s)	581, 635, 748, 784, 832, 949, 1012, 1286, 1352
FaqI	GGGAC	2 cut(s)	135, 1330
FatI	CATG	9 cut(s)	577, 631, 744, 780, 828, 945, 1008, 1282, 1348
FbaI	TGATCA	1 cut(s)	1000
Fnu4HI	GCNGC	4 cut(s)	129, 324, 570, 730
FokI	GGATG	6 cut(s)	479, 488, 500, 773, 962, 1381
FriOI	GRGCYC	2 cut(s)	294, 661
Fsp4HI	GCNGC	4 cut(s)	129, 324, 570, 730
FspBI	CTAG	4 cut(s)	170, 216, 494, 1052
GluI	GCNGC	4 cut(s)	129, 324, 570, 730
GsuI	CTGGAG	1 cut(s)	1437
HaeIII	GGCC	5 cut(s)	58, 544, 601, 659, 779
HapII	CCGG	2 cut(s)	304, 817
Hin1I	GRCGYC	1 cut(s)	5
Hin1II	CATG	9 cut(s)	581, 635, 748, 784, 832, 949, 1012, 1286, 1352
HinfI	GANTC	4 cut(s)	612, 920, 1141, 1194
HpaII	CCGG	2 cut(s)	304, 817
HphI	GGTGA	3 cut(s)	211, 296, 382
Hpy166II	GTNNAC	3 cut(s)	453, 1078, 1439
Hpy188I	TCNGA	9 cut(s)	157, 237, 253, 566, 937, 1108, 1150, 1207, 1402
Hpy188III	TCNNGA	3 cut(s)	170, 494, 1416
Hpy8I	GTNNAC	3 cut(s)	453, 1078, 1439
Hpy99I	CGWCG	1 cut(s)	10
HpyAV	CCTTC	6 cut(s)	361, 388, 466, 952, 1091, 1212
HpyCH4III	ACNGT	2 cut(s)	110, 1092
HpyCH4IV	ACGT	2 cut(s)	229, 313
HpyCH4V	TGCA	9 cut(s)	85, 131, 176, 326, 650, 695, 719, 851, 1271
HpyF10VI	GCNNNNNNNGC	5 cut(s)	64, 173, 329, 578, 656
HpyF3I	CTNAG	9 cut(s)	98, 360, 387, 444, 616, 915, 1167, 1206, 1323
HpySE526I	ACGT	2 cut(s)	229, 313
Hsp92I	GRCGYC	1 cut(s)	5
Hsp92II	CATG	9 cut(s)	581, 635, 748, 784, 832, 949, 1012, 1286, 1352
Ksp22I	TGATCA	1 cut(s)	1000
Kzo9I	GATC	7 cut(s)	490, 689, 867, 1000, 1013, 1235, 1375
LguI	GCTCTTC	2 cut(s)	282, 835
LmnI	GCTCC	2 cut(s)	566, 1345
Lsp1109I	GCAGC	3 cut(s)	115, 310, 556
LweI	GCATC	6 cut(s)	163, 257, 335, 421, 637, 1421
MaeI	CTAG	4 cut(s)	170, 216, 494, 1052
MaeII	ACGT	2 cut(s)	229, 313
MaeIII	GTNAC	3 cut(s)	69, 1162, 1306
MalI	GATC	7 cut(s)	492, 691, 869, 1002, 1015, 1237, 1377
MboI	GATC	7 cut(s)	490, 689, 867, 1000, 1013, 1235, 1375
MflI	RGATCY	2 cut(s)	689, 1375
MhlI	GDGCHC	2 cut(s)	294, 661
MluCI	AATT	8 cut(s)	32, 76, 151, 160, 497, 722, 1038, 1211
MlyI	GAGTC	2 cut(s)	606, 1203
MmeI	TCCRAC	1 cut(s)	915
Mph1103I	ATGCAT	1 cut(s)	652
MseI	TTAA	4 cut(s)	75, 435, 1293, 1362
MslI	CAYNNNNRTG	3 cut(s)	950, 1353, 1444
MspA1I	CMGCKG	1 cut(s)	528
MspCI	CTTAAG	1 cut(s)	1292
MspI	CCGG	2 cut(s)	304, 817
MspR9I	CCNGG	5 cut(s)	40, 304, 305, 684, 817
Mva1269I	GAATGC	1 cut(s)	802
MvaI	CCWGG	2 cut(s)	40, 684
MwoI	GCNNNNNNNGC	5 cut(s)	64, 173, 329, 578, 656
NciI	CCSGG	3 cut(s)	304, 305, 817
NdeII	GATC	7 cut(s)	490, 689, 867, 1000, 1013, 1235, 1375
NlaIII	CATG	9 cut(s)	581, 635, 748, 784, 832, 949, 1012, 1286, 1352
NlaIV	GGNNCC	4 cut(s)	44, 659, 660, 1377
NmuCI	GTSAC	2 cut(s)	69, 1306
NsiI	ATGCAT	1 cut(s)	652
NspI	RCATGY	1 cut(s)	581
OliI	CACNNNNGTG	1 cut(s)	1444
PciSI	GCTCTTC	2 cut(s)	282, 835
PctI	GAATGC	1 cut(s)	802
PfeI	GAWTC	2 cut(s)	920, 1141
PkrI	GCNGC	4 cut(s)	130, 325, 571, 731
PleI	GAGTC	2 cut(s)	606, 1202
PpsI	GAGTC	2 cut(s)	606, 1202
PshAI	GACNNNNGTC	1 cut(s)	899
Psp6I	CCWGG	2 cut(s)	38, 682
PspGI	CCWGG	2 cut(s)	38, 682
PspN4I	GGNNCC	4 cut(s)	44, 659, 660, 1377
PspOMI	GGGCCC	1 cut(s)	657
PspPI	GGNCC	3 cut(s)	57, 657, 658
PstI	CTGCAG	2 cut(s)	697, 853
PstNI	CAGNNNCTG	1 cut(s)	1415
PsuI	RGATCY	2 cut(s)	689, 1375
PvuII	CAGCTG	1 cut(s)	528
RsaI	GTAC	3 cut(s)	232, 532, 1438
RsaNI	GTAC	3 cut(s)	231, 531, 1437
RseI	CAYNNNNRTG	3 cut(s)	950, 1353, 1444
SapI	GCTCTTC	2 cut(s)	282, 835
SaqAI	TTAA	4 cut(s)	75, 435, 1293, 1362
SatI	GCNGC	4 cut(s)	129, 324, 570, 730
Sau3AI	GATC	7 cut(s)	490, 689, 867, 1000, 1013, 1235, 1375
Sau96I	GGNCC	3 cut(s)	57, 657, 658
SchI	GAGTC	2 cut(s)	606, 1203
ScrFI	CCNGG	5 cut(s)	40, 304, 305, 684, 817
SduI	GDGCHC	2 cut(s)	294, 661
SfaNI	GCATC	6 cut(s)	163, 257, 335, 421, 637, 1421
SfcI	CTRYAG	2 cut(s)	693, 849
SmaI	CCCGGG	1 cut(s)	305
SmiMI	CAYNNNNRTG	3 cut(s)	950, 1353, 1444
SmlI	CTYRAG	3 cut(s)	886, 1178, 1292
SmoI	CTYRAG	3 cut(s)	886, 1178, 1292
Sse9I	AATT	8 cut(s)	32, 76, 151, 160, 497, 722, 1038, 1211
SsiI	CCGC	1 cut(s)	729
SspMI	CTAG	4 cut(s)	170, 216, 494, 1052
StyD4I	CCNGG	5 cut(s)	38, 302, 303, 682, 815
TaaI	ACNGT	2 cut(s)	110, 1092
TaiI	ACGT	2 cut(s)	232, 316
TaqI	TCGA	3 cut(s)	266, 1215, 1238
TasI	AATT	8 cut(s)	32, 76, 151, 160, 497, 722, 1038, 1211
TatI	WGTACW	1 cut(s)	530
TauI	GCSGC	1 cut(s)	732
TfiI	GAWTC	2 cut(s)	920, 1141
Tru1I	TTAA	4 cut(s)	75, 435, 1293, 1362
Tru9I	TTAA	4 cut(s)	75, 435, 1293, 1362
TscAI	CASTG	1 cut(s)	706
TseFI	GTSAC	2 cut(s)	69, 1306
TseI	GCWGC	3 cut(s)	128, 323, 569
Tsp45I	GTSAC	2 cut(s)	69, 1306
TspMI	CCCGGG	1 cut(s)	303
TspRI	CASTG	1 cut(s)	706
Vha464I	CTTAAG	1 cut(s)	1292
XapI	RAATTY	3 cut(s)	160, 1038, 1211
XbaI	TCTAGA	2 cut(s)	169, 493
XceI	RCATGY	1 cut(s)	581
XcmI	CCANNNNNNNNNTGG	2 cut(s)	46, 1092
XmaI	CCCGGG	1 cut(s)	303
XspI	CTAG	4 cut(s)	170, 216, 494, 1052
Zsp2I	ATGCAT	1 cut(s)	652

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.