Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr13

Orthogroup ID

OG0007179

Physical Location & Seq

Genomic Coordinates Forward (+)

5400078 .. 5404191

Gene Structure 4114 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD13G1076100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1260 bp

ATGGGCAACTGTTTAGGTTCTTCTGCTAAAGTCGATACCGCACATAGCTCCTTTGGAACTTCGGGATCAGGGGTTTCCAAAGTTTCCAGCAAAACTAACCCTTCCTCAGTTCCCTCCACTTTGACCGTCCCATCGTATAGTGGAAGGAGCAATGCTTCAAGTCTTCCTACCCCAAGGACTGAAGGCGAAATATTGTCATGTCCAAATTTGAAACCCTTCTCATTCAATGAGCTAAAGACTGCCACCAGAAACTTTCGCCCTGACAGTCTTCTCGGTGAAGGAGGATTTGGTTATGTTTTCAAAGGATGGATTAATGGGGGCACAATGACAGCTGCAAAACCGGGGTCCGGAATGGTTGTTGCTGTCAAGAAGCTCAAACCTGAAGGTTTCCAAGGCCACAAGGAGTGGTTGACAGAAGTAAATTATCTTGGTCAACTCCATCATCCAAATCTGGTTAAGCTTATCGGATACTGCTTAGAGGGTGAGAACCGCCTTCTGGTGTATGAGTTTTTGCCAAAAGGAAGCCTAGAGAATCATCTGTTTAGAAGAGGGCCGCAACCACTTTCATGGCCAACAAGAATGAAAGTAGCCATAGGTGCTGCAAAGGGGCTCAGTTTTCTTCATGAGGCAGAGTCACAAGTCATATATCGTGATTTTAAGGCTTCTAATATTTTATTAGATTCGGAATTCAACGCGAAGCTTTCTGATTTTGGCTTGGCTAAGGCTGGTCCGACTGGTGACAGGACTCACGTGTCTACTCAAGTCATGGGTACTCAAGGCTACGCAGCACCCGAATATGTTGCTACAGGTAGATTGACTGCCAAAAGTGACGTCTATAGTTTTGGGGTTGTACTGCTGGAACTACTGTCTGGAAGGCGAGCTGTTGATAAAACGAAAGTTGGTGCGGAGCAGAACCTGGTAGACTGGACAAAACCATATTTGGGTGACAGAAGAAAACTATTTCGGATTATGGACACCAAGTTGGAGGGCCAATATCCCCAGAAAGCAGCTTATACAGCTGCTACCCTTGCTTCACAATGCCTATGCCCCGAATCTAAACTCAGGCCCACAATGTCAGTGGTATTAGCAACGCTGGAACAACTTGAATCCCCAAAGACTGCAGCCAGAAACTCTCAATCAGAACAGCAGACACTTCGTATTCCTCGGAAGTCGCCAATGCGACAACACCGTTCTCCTCTCAATGTACCACCAAACGCATCCCCATTGGCATACCAAAGGCAATCTCCACGCGTGCGTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004675 GO:0004888 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0007166 GO:0007167 GO:0007178 GO:0008134 GO:0008150 GO:0008152 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019199 GO:0019538 GO:0023052 GO:0030957 GO:0036211 GO:0038023 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0060089 GO:0065007 GO:0071704 GO:0071944 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko04010 ko04064 ko04620 ko04621 ko04624 ko04722 ko05133 ko05140 ko05142 ko05145 ko05152 ko05162 ko05164 map04010 map04064 map04620 map04621 map04624 map04722 map05133 map05140 map05142 map05145 map05152 map05162 map05164

Pfam Domains

Protein Families

Pkinase_Tyr

Protein Analysis

420

Amino Acids

45.71

Weight (kDa)

9.74

Isoelectric Point (pI)

37.0

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	88 - 362	8.1e-44	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	89 - 363	4.7e-46	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007179)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G14370 AT1G26970 AT1G26970 AT1G69790 AT1G69790 AT2G02800 AT2G02800
fragaria_vesca	FvH4_4g31680 FvH4_4g31680 FvH4_4g31680
malus_domestica	MD13G1076100.v1.1 MD16G1076300.v1.1
prunus_persica	Prupe.1G270700_v2.0.a1 Prupe.1G270700_v2.0.a1
pyrus_communis	pycom16g06630
rosa_chinensis	RchiOBHm_Chr4g0440191
rosa_laevigata	RLG00000006197
rosa_multiflora	Rmu_sc0001685.1_g000027
rosa_roxburghii	Rroxscaffold_5G00381120
rosa_rugosa	Rorug04G0325100
rosa_samantha	Rh4AG375900 Rh4BG388900 Rh4CG403400 Rh4DG382600
rosa_wichuraiana	Rw4G032170

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	751
AatII	GACGTC	1 cut(s)	834
AccI	GTMKAC	2 cut(s)	755, 921
AccII	CGCG	2 cut(s)	695, 1251
AccIII	TCCGGA	1 cut(s)	347
AciI	CCGC	4 cut(s)	39, 490, 554, 905
AclWI	GGATC	1 cut(s)	73
AcoI	YGGCCR	1 cut(s)	569
AcsI	RAATTY	2 cut(s)	205, 686
AcuI	CTGAAG	2 cut(s)	201, 402
AcvI	CACGTG	1 cut(s)	751
AcyI	GRCGYC	1 cut(s)	831
AfaI	GTAC	3 cut(s)	772, 852, 1206
AfiI	CCNNNNNNNGG	3 cut(s)	347, 496, 941
AflIII	ACRYGT	2 cut(s)	750, 1249
AgsI	TTSAA	6 cut(s)	159, 211, 226, 301, 691, 1106
AjnI	CCWGG	1 cut(s)	915
AjuI	GAANNNNNNNTTGG	2 cut(s)	270, 302
AluBI	AGCT	9 cut(s)	48, 232, 332, 373, 460, 700, 881, 1010, 1019
AluI	AGCT	9 cut(s)	48, 232, 332, 373, 460, 700, 881, 1010, 1019
AlwI	GGATC	1 cut(s)	73
AlwNI	CAGNNNCTG	1 cut(s)	916
Aor13HI	TCCGGA	1 cut(s)	347
AoxI	GGCC	5 cut(s)	394, 551, 569, 988, 1064
ApeKI	GCWGC	6 cut(s)	332, 599, 785, 1007, 1019, 1121
ApoI	RAATTY	2 cut(s)	205, 686
ArsI	GACNNNNNNTTYG	4 cut(s)	329, 361, 816, 848
AseI	ATTAAT	1 cut(s)	312
Asp700I	GAANNNNTTC	1 cut(s)	215
AspS9I	GGNCC	5 cut(s)	345, 551, 728, 988, 1065
AsuC2I	CCSGG	1 cut(s)	342
AsuHPI	GGTGA	4 cut(s)	287, 494, 749, 956
AvaII	GGWCC	2 cut(s)	345, 728
BaeGI	GKGCMC	1 cut(s)	323
BalI	TGGCCA	1 cut(s)	571
BanII	GRGCYC	1 cut(s)	612
BbrPI	CACGTG	1 cut(s)	751
BbsI	GAAGAC	2 cut(s)	155, 260
BbvI	GCAGC	6 cut(s)	319, 586, 797, 1006, 1019, 1133
BccI	CCATC	3 cut(s)	139, 300, 447
BcgI	CGANNNNNNTGC	4 cut(s)	782, 816, 1136, 1170
BciT130I	CCWGG	1 cut(s)	917
BciVI	GTATCC	1 cut(s)	461
BcnI	CCSGG	1 cut(s)	342
BfaI	CTAG	1 cut(s)	527
BfmI	CTRYAG	3 cut(s)	804, 835, 1119
BfuI	GTATCC	1 cut(s)	461
BisI	GCNGC	7 cut(s)	333, 554, 600, 786, 1008, 1020, 1122
BlsI	GCNGC	7 cut(s)	334, 555, 601, 787, 1009, 1021, 1123
Bme1390I	CCNGG	2 cut(s)	342, 917
Bme18I	GGWCC	2 cut(s)	345, 728
BmgT120I	GGNCC	5 cut(s)	345, 551, 728, 988, 1065
BmiI	GGNNCC	1 cut(s)	346
BmrFI	CCNGG	2 cut(s)	342, 917
BmsI	GCATC	1 cut(s)	1226
BpiI	GAAGAC	2 cut(s)	155, 260
Bpu10I	CCTNAGC	1 cut(s)	720
BpuEI	CTTGAG	2 cut(s)	744, 759
BpuMI	CCSGG	1 cut(s)	342
BsaAI	YACGTR	1 cut(s)	751
BsaHI	GRCGYC	1 cut(s)	831
BsaJI	CCNNGG	4 cut(s)	173, 341, 391, 1163
BsaWI	WCCGGW	1 cut(s)	347
Bsc4I	CCNNNNNNNGG	3 cut(s)	347, 496, 941
Bse1I	ACTGG	2 cut(s)	739, 929
Bse3DI	GCAATG	1 cut(s)	157
BseAI	TCCGGA	1 cut(s)	347
BseBI	CCWGG	1 cut(s)	917
BseDI	CCNNGG	4 cut(s)	173, 341, 391, 1163
BseGI	GGATG	3 cut(s)	311, 442, 1217
BseLI	CCNNNNNNNGG	3 cut(s)	347, 496, 941
BseMI	GCAATG	1 cut(s)	157
BseMII	CTCAG	3 cut(s)	120, 625, 1075
BseNI	ACTGG	2 cut(s)	739, 929
BseRI	GAGGAG	1 cut(s)	1185
BseSI	GKGCMC	1 cut(s)	323
BseXI	GCAGC	6 cut(s)	319, 586, 797, 1006, 1019, 1133
Bsh1236I	CGCG	2 cut(s)	695, 1251
BshFI	GGCC	5 cut(s)	396, 553, 571, 990, 1066
BsiSI	CCGG	2 cut(s)	341, 348
BslFI	GGGAC	1 cut(s)	113
BslI	CCNNNNNNNGG	3 cut(s)	347, 496, 941
BsmFI	GGGAC	1 cut(s)	113
BsnI	GGCC	5 cut(s)	396, 553, 571, 990, 1066
Bsp1286I	GDGCHC	2 cut(s)	323, 612
Bsp13I	TCCGGA	1 cut(s)	347
Bsp143I	GATC	1 cut(s)	65
BspACI	CCGC	4 cut(s)	39, 490, 554, 905
BspANI	GGCC	5 cut(s)	396, 553, 571, 990, 1066
BspCNI	CTCAG	3 cut(s)	119, 624, 1074
BspEI	TCCGGA	1 cut(s)	347
BspFNI	CGCG	2 cut(s)	695, 1251
BspHI	TCATGA	1 cut(s)	622
BspLI	GGNNCC	1 cut(s)	346
BspMAI	CTGCAG	1 cut(s)	1123
BspPI	GGATC	1 cut(s)	73
BsrDI	GCAATG	1 cut(s)	157
BsrI	ACTGG	2 cut(s)	739, 929
BssECI	CCNNGG	4 cut(s)	173, 341, 391, 1163
BssMI	GATC	1 cut(s)	65
BssNI	GRCGYC	1 cut(s)	831
BssT1I	CCWWGG	2 cut(s)	173, 391
Bst2UI	CCWGG	1 cut(s)	917
Bst4CI	ACNGT	5 cut(s)	11, 127, 266, 867, 1190
Bst6I	CTCTTC	1 cut(s)	541
BstACI	GRCGYC	1 cut(s)	831
BstBAI	YACGTR	1 cut(s)	751
BstC8I	GCNNGC	2 cut(s)	879, 1253
BstDEI	CTNAG	5 cut(s)	106, 475, 611, 720, 1061
BstF5I	GGATG	3 cut(s)	311, 442, 1217
BstFNI	CGCG	2 cut(s)	695, 1251
BstKTI	GATC	1 cut(s)	68
BstMBI	GATC	1 cut(s)	65
BstMWI	GCNNNNNNNGC	3 cut(s)	596, 1016, 1028
BstNI	CCWGG	1 cut(s)	917
BstSCI	CCNGG	2 cut(s)	340, 915
BstSFI	CTRYAG	3 cut(s)	804, 835, 1119
BstSLI	GKGCMC	1 cut(s)	323
BstUI	CGCG	2 cut(s)	695, 1251
BstV1I	GCAGC	6 cut(s)	319, 586, 797, 1006, 1019, 1133
BstV2I	GAAGAC	2 cut(s)	155, 260
BstXI	CCANNNNNNTGG	1 cut(s)	567
BsuI	GTATCC	1 cut(s)	461
BsuRI	GGCC	5 cut(s)	396, 553, 571, 990, 1066
BtsCI	GGATG	3 cut(s)	311, 442, 1217
BtsIMutI	CAGTG	1 cut(s)	1083
Cac8I	GCNNGC	2 cut(s)	879, 1253
CaiI	CAGNNNCTG	1 cut(s)	916
CciI	TCATGA	1 cut(s)	622
Cfr13I	GGNCC	5 cut(s)	345, 551, 728, 988, 1065
CsiI	ACCWGGT	1 cut(s)	915
Csp6I	GTAC	3 cut(s)	771, 851, 1205
CspCI	CAANNNNNGTGG	2 cut(s)	1059, 1094
CviAII	CATG	4 cut(s)	198, 567, 623, 766
CviQI	GTAC	3 cut(s)	771, 851, 1205
DdeI	CTNAG	5 cut(s)	106, 475, 611, 720, 1061
DpnI	GATC	1 cut(s)	67
DpnII	GATC	1 cut(s)	65
DrdI	GACNNNNNNGTC	1 cut(s)	751
DseDI	GACNNNNNNGTC	1 cut(s)	751
EaeI	YGGCCR	1 cut(s)	569
Eam1104I	CTCTTC	1 cut(s)	541
EarI	CTCTTC	1 cut(s)	541
Eco130I	CCWWGG	2 cut(s)	173, 391
Eco24I	GRGCYC	1 cut(s)	612
Eco47I	GGWCC	2 cut(s)	345, 728
Eco57I	CTGAAG	2 cut(s)	201, 402
Eco72I	CACGTG	1 cut(s)	751
EcoRI	GAATTC	1 cut(s)	686
EcoRII	CCWGG	1 cut(s)	915
EcoT14I	CCWWGG	2 cut(s)	173, 391
EcoT38I	GRGCYC	1 cut(s)	612
ErhI	CCWWGG	2 cut(s)	173, 391
FaeI	CATG	4 cut(s)	201, 570, 626, 769
FaqI	GGGAC	1 cut(s)	113
FatI	CATG	4 cut(s)	197, 566, 622, 765
FblI	GTMKAC	2 cut(s)	755, 921
Fnu4HI	GCNGC	7 cut(s)	333, 554, 600, 786, 1008, 1020, 1122
FokI	GGATG	3 cut(s)	318, 429, 1204
FriOI	GRGCYC	1 cut(s)	612
Fsp4HI	GCNGC	7 cut(s)	333, 554, 600, 786, 1008, 1020, 1122
FspBI	CTAG	1 cut(s)	527
GluI	GCNGC	7 cut(s)	333, 554, 600, 786, 1008, 1020, 1122
HaeIII	GGCC	5 cut(s)	396, 553, 571, 990, 1066
HapII	CCGG	2 cut(s)	341, 348
Hin1I	GRCGYC	1 cut(s)	831
Hin1II	CATG	4 cut(s)	201, 570, 626, 769
HincII	GTYRAC	2 cut(s)	411, 434
HindII	GTYRAC	2 cut(s)	411, 434
HindIII	AAGCTT	2 cut(s)	458, 698
HinfI	GANTC	6 cut(s)	532, 632, 680, 745, 1052, 1106
HpaII	CCGG	2 cut(s)	341, 348
HphI	GGTGA	4 cut(s)	287, 494, 749, 956
Hpy166II	GTNNAC	4 cut(s)	411, 434, 756, 922
Hpy188I	TCNGA	7 cut(s)	467, 685, 706, 732, 966, 1141, 1167
Hpy188III	TCNNGA	6 cut(s)	63, 348, 367, 623, 650, 870
Hpy8I	GTNNAC	4 cut(s)	411, 434, 756, 922
HpyAV	CCTTC	8 cut(s)	111, 138, 176, 226, 272, 377, 503, 867
HpyCH4III	ACNGT	5 cut(s)	11, 127, 266, 867, 1190
HpyCH4IV	ACGT	2 cut(s)	750, 831
HpyCH4V	TGCA	3 cut(s)	335, 602, 1121
HpyF10VI	GCNNNNNNNGC	3 cut(s)	596, 1016, 1028
HpyF3I	CTNAG	5 cut(s)	106, 475, 611, 720, 1061
HpySE526I	ACGT	2 cut(s)	750, 831
Hsp92I	GRCGYC	1 cut(s)	831
Hsp92II	CATG	4 cut(s)	201, 570, 626, 769
Kpn2I	TCCGGA	1 cut(s)	347
Kzo9I	GATC	1 cut(s)	65
LmnI	GCTCC	3 cut(s)	53, 147, 907
Lsp1109I	GCAGC	6 cut(s)	319, 586, 797, 1006, 1019, 1133
LweI	GCATC	1 cut(s)	1226
MabI	ACCWGGT	1 cut(s)	915
MaeI	CTAG	1 cut(s)	527
MaeII	ACGT	2 cut(s)	750, 831
MaeIII	GTNAC	4 cut(s)	633, 737, 827, 944
MalI	GATC	1 cut(s)	67
MboI	GATC	1 cut(s)	65
MboII	GAAGA	6 cut(s)	12, 155, 260, 558, 611, 963
MhlI	GDGCHC	2 cut(s)	323, 612
MlsI	TGGCCA	1 cut(s)	571
MluCI	AATT	3 cut(s)	205, 421, 686
MluI	ACGCGT	1 cut(s)	1249
MluNI	TGGCCA	1 cut(s)	571
MlyI	GAGTC	2 cut(s)	641, 739
MmeI	TCCRAC	2 cut(s)	755, 963
MnlI	CCTC	9 cut(s)	115, 124, 275, 472, 542, 619, 979, 1173, 1206
Mox20I	TGGCCA	1 cut(s)	571
MroI	TCCGGA	1 cut(s)	347
MroXI	GAANNNNTTC	1 cut(s)	215
MscI	TGGCCA	1 cut(s)	571
MseI	TTAA	3 cut(s)	312, 456, 657
MslI	CAYNNNNRTG	1 cut(s)	565
Msp20I	TGGCCA	1 cut(s)	571
MspA1I	CMGCKG	2 cut(s)	332, 1019
MspI	CCGG	2 cut(s)	341, 348
MspR9I	CCNGG	2 cut(s)	342, 917
MvaI	CCWGG	1 cut(s)	917
MvnI	CGCG	2 cut(s)	695, 1251
MwoI	GCNNNNNNNGC	3 cut(s)	596, 1016, 1028
NciI	CCSGG	1 cut(s)	342
NdeII	GATC	1 cut(s)	65
NlaIII	CATG	4 cut(s)	201, 570, 626, 769
NlaIV	GGNNCC	1 cut(s)	346
NmuCI	GTSAC	4 cut(s)	633, 737, 827, 944
PagI	TCATGA	1 cut(s)	622
PcsI	WCGNNNNNNNCGW	1 cut(s)	789
PdmI	GAANNNNTTC	1 cut(s)	215
PfeI	GAWTC	4 cut(s)	532, 680, 1052, 1106
PkrI	GCNGC	7 cut(s)	334, 555, 601, 787, 1009, 1021, 1123
PleI	GAGTC	2 cut(s)	640, 739
PmaCI	CACGTG	1 cut(s)	751
PmlI	CACGTG	1 cut(s)	751
PpsI	GAGTC	2 cut(s)	640, 739
Ppu21I	YACGTR	1 cut(s)	751
PshBI	ATTAAT	1 cut(s)	312
Psp6I	CCWGG	1 cut(s)	915
PspCI	CACGTG	1 cut(s)	751
PspGI	CCWGG	1 cut(s)	915
PspN4I	GGNNCC	1 cut(s)	346
PspPI	GGNCC	5 cut(s)	345, 551, 728, 988, 1065
PstI	CTGCAG	1 cut(s)	1123
PstNI	CAGNNNCTG	1 cut(s)	916
PvuII	CAGCTG	2 cut(s)	332, 1019
RsaI	GTAC	3 cut(s)	772, 852, 1206
RsaNI	GTAC	3 cut(s)	771, 851, 1205
RseI	CAYNNNNRTG	1 cut(s)	565
SaqAI	TTAA	3 cut(s)	312, 456, 657
SatI	GCNGC	7 cut(s)	333, 554, 600, 786, 1008, 1020, 1122
Sau3AI	GATC	1 cut(s)	65
Sau96I	GGNCC	5 cut(s)	345, 551, 728, 988, 1065
SchI	GAGTC	2 cut(s)	641, 739
ScrFI	CCNGG	2 cut(s)	342, 917
SduI	GDGCHC	2 cut(s)	323, 612
SexAI	ACCWGGT	1 cut(s)	915
SfaNI	GCATC	1 cut(s)	1226
SfcI	CTRYAG	3 cut(s)	804, 835, 1119
SinI	GGWCC	2 cut(s)	345, 728
SmiMI	CAYNNNNRTG	1 cut(s)	565
SmlI	CTYRAG	2 cut(s)	759, 774
SmoI	CTYRAG	2 cut(s)	759, 774
Sse9I	AATT	3 cut(s)	205, 421, 686
SsiI	CCGC	4 cut(s)	39, 490, 554, 905
SspI	AATATT	2 cut(s)	192, 670
SspMI	CTAG	1 cut(s)	527
StyD4I	CCNGG	2 cut(s)	340, 915
StyI	CCWWGG	2 cut(s)	173, 391
TaaI	ACNGT	5 cut(s)	11, 127, 266, 867, 1190
TaiI	ACGT	2 cut(s)	753, 834
TaqI	TCGA	1 cut(s)	33
TasI	AATT	3 cut(s)	205, 421, 686
TatI	WGTACW	1 cut(s)	850
TauI	GCSGC	1 cut(s)	556
TfiI	GAWTC	4 cut(s)	532, 680, 1052, 1106
Tru1I	TTAA	3 cut(s)	312, 456, 657
Tru9I	TTAA	3 cut(s)	312, 456, 657
TscAI	CASTG	1 cut(s)	1083
TseFI	GTSAC	4 cut(s)	633, 737, 827, 944
TseI	GCWGC	6 cut(s)	332, 599, 785, 1007, 1019, 1121
Tsp45I	GTSAC	4 cut(s)	633, 737, 827, 944
TspDTI	ATGAA	3 cut(s)	555, 596, 611
TspRI	CASTG	1 cut(s)	1083
VpaK11BI	GGWCC	2 cut(s)	345, 728
VspI	ATTAAT	1 cut(s)	312
XapI	RAATTY	2 cut(s)	205, 686
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1075
XmiI	GTMKAC	2 cut(s)	755, 921
XmnI	GAANNNNTTC	1 cut(s)	215
XspI	CTAG	1 cut(s)	527
ZraI	GACGTC	1 cut(s)	832

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.