Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0007179

Physical Location & Seq

Genomic Coordinates Forward (+)

5368653 .. 5372676

Gene Structure 4024 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1076300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1260 bp

ATGGGCAACTGTTTAGATTCTTCTGCTAAAGTCGACACCGCACAGAGCTCCCTTGGAACTTGGGGATCAGGGGCTTCCAAAGTTTCCAACAAAACTAGCCCTTCCTCAGCTCCCTCCACTTTGACCGTCCCGTCATATAATGGAAGGAGCAGTGGTTCAAGTCTTCCTACCCCAAGGACTGAAGGAGAAATATTGTCATCTCCAAATTTGAAAGCCTTCTCATTCAATGAGCTAAAGAATGCCACCCGAAACTTCCGCCCTGACAGTCTTCTCGGTGAAGGAGGATTTGGTTATGTTTTCAAAGGATGGATTAATGAGAACACGTGGACAGCTGCAAAACCAGGGTCCGGAATGGTTGTTGCTGTCAAGAAGCTCAAACCTGAGGGTTTCCAAGGCCACAAGGAGTGGTTGACAGAAGTAAATTATCTTGGTCAACTCCATCATCCAAATCTGGTTAAGCTTATCGGGTACTGCTTGGAGGGTGAGAACCGCCTTTTGGTGTATGAGTTCATGCCGAAAGGAAGCTTAGAGAATCATCTGTTTAGAAGAGGGCCGCAACCACTTTCATGGGCAACAAGAATGAAAGTAGCCATAGGCGCTGCAAGGGGGCTCAGTTTTCTTCACGAGGCGGAATCACAAGTCATATATCGTGATTTTAAGGCTTCCAATATTTTACTAGATTCGGAATTCAACGCGAAGCTTTCTGATTTTGGCTTGGCCAAGGCTGGTCCCACTGGTGACAGGACTCATGTGTCTACTCAAGTCATGGGTACTCAAGGCTATGCTGCACCCGAATATGTAGCTACAGGTAGGTTGACTGCTAAAAGTGATGTCTATAGTTTTGGGGTTGTACTGCTGGAACTATTGTCTGGAAGGCGAGCTGTTGATAAAACAAAAGTTGGTGTGGAGCAGAATCTGGTAGATTGGACGAAACCATATTTGGGTGATAAAAGAAAACTATTTCGGATTATGGATACCAAGTTGGAGGGCCAATATCCCCAGAAAGCAGCTTATACAGCTGCTACTCTTGCTTCACAATGCCTATGCACCGAATCTAAACTCAGGCCTACAATGTCAGTGGTATTAGCCACGCTGGAACAACTTGAATCCCCAAAGACTGCAGCCAGAAACTCTCAATCAGAACAGCACACACTTCCTGTTCCTCGGAAGTCGCCAATGCGACAACACCATTCTCCTCTCAATATGCCACCAAATGCATCCCCATTGGCATCCCAAAGGCAATCTGTACGTGTTCGTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004675 GO:0004888 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0007166 GO:0007167 GO:0007178 GO:0008134 GO:0008150 GO:0008152 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019199 GO:0019538 GO:0023052 GO:0030957 GO:0036211 GO:0038023 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0060089 GO:0065007 GO:0071704 GO:0071944 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko04010 ko04064 ko04620 ko04621 ko04624 ko04722 ko05133 ko05140 ko05142 ko05145 ko05152 ko05162 ko05164 map04010 map04064 map04620 map04621 map04624 map04722 map05133 map05140 map05142 map05145 map05152 map05162 map05164

Pfam Domains

Protein Families

Pkinase_Tyr

Protein Analysis

420

Amino Acids

45.81

Weight (kDa)

9.62

Isoelectric Point (pI)

38.65

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	88 - 362	7.8e-45	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	89 - 363	5.3e-47	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007179)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G14370 AT1G26970 AT1G26970 AT1G69790 AT1G69790 AT2G02800 AT2G02800
fragaria_vesca	FvH4_4g31680 FvH4_4g31680 FvH4_4g31680
malus_domestica	MD13G1076100.v1.1 MD16G1076300.v1.1
prunus_persica	Prupe.1G270700_v2.0.a1 Prupe.1G270700_v2.0.a1
pyrus_communis	pycom16g06630
rosa_chinensis	RchiOBHm_Chr4g0440191
rosa_laevigata	RLG00000006197
rosa_multiflora	Rmu_sc0001685.1_g000027
rosa_roxburghii	Rroxscaffold_5G00381120
rosa_rugosa	Rorug04G0325100
rosa_samantha	Rh4AG375900 Rh4BG388900 Rh4CG403400 Rh4DG382600
rosa_wichuraiana	Rw4G032170

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	2 cut(s)	130, 751
AccI	GTMKAC	2 cut(s)	33, 755
AccII	CGCG	1 cut(s)	695
AccIII	TCCGGA	1 cut(s)	347
AciI	CCGC	5 cut(s)	39, 256, 490, 554, 629
AclWI	GGATC	1 cut(s)	73
AcoI	YGGCCR	1 cut(s)	717
AcsI	RAATTY	2 cut(s)	205, 686
AcuI	CTGAAG	1 cut(s)	201
AcvI	CACGTG	1 cut(s)	324
AfaI	GTAC	4 cut(s)	470, 772, 852, 1248
AfiI	CCNNNNNNNGG	3 cut(s)	347, 496, 941
AflIII	ACRYGT	2 cut(s)	321, 1249
AgsI	TTSAA	6 cut(s)	159, 211, 226, 301, 691, 1106
AjnI	CCWGG	1 cut(s)	340
AjuI	GAANNNNNNNTTGG	6 cut(s)	270, 302, 479, 511, 923, 955
AloI	GAACNNNNNNTCC	2 cut(s)	139, 171
Alw21I	GWGCWC	1 cut(s)	50
AlwI	GGATC	1 cut(s)	73
AlwNI	CAGNNNCTG	1 cut(s)	916
Aor13HI	TCCGGA	1 cut(s)	347
AoxI	GGCC	5 cut(s)	394, 551, 717, 988, 1064
ApeKI	GCWGC	6 cut(s)	332, 599, 785, 1007, 1019, 1121
ApoI	RAATTY	2 cut(s)	205, 686
ArsI	GACNNNNNNTTYG	2 cut(s)	329, 361
AseI	ATTAAT	1 cut(s)	312
Asp700I	GAANNNNTTC	1 cut(s)	215
AspLEI	GCGC	1 cut(s)	599
AspS9I	GGNCC	4 cut(s)	345, 551, 728, 988
AsuHPI	GGTGA	4 cut(s)	287, 494, 749, 956
AvaII	GGWCC	2 cut(s)	345, 728
AxyI	CCTNAGG	1 cut(s)	381
BalI	TGGCCA	1 cut(s)	719
BanII	GRGCYC	2 cut(s)	50, 612
BauI	CACGAG	1 cut(s)	623
BbrPI	CACGTG	1 cut(s)	324
BbsI	GAAGAC	2 cut(s)	155, 260
Bbv12I	GWGCWC	1 cut(s)	50
BbvCI	CCTCAGC	1 cut(s)	106
BbvI	GCAGC	6 cut(s)	319, 586, 772, 1006, 1019, 1133
BccI	CCATC	2 cut(s)	300, 447
BciT130I	CCWGG	1 cut(s)	342
BciVI	GTATCC	1 cut(s)	967
BfaI	CTAG	2 cut(s)	96, 677
BfmI	CTRYAG	3 cut(s)	804, 835, 1119
BfoI	RGCGCY	1 cut(s)	600
BfuI	GTATCC	1 cut(s)	967
BisI	GCNGC	7 cut(s)	333, 554, 600, 786, 1008, 1020, 1122
BlsI	GCNGC	7 cut(s)	334, 555, 601, 787, 1009, 1021, 1123
Bme1390I	CCNGG	1 cut(s)	342
Bme18I	GGWCC	2 cut(s)	345, 728
BmgT120I	GGNCC	4 cut(s)	345, 551, 728, 988
BmiI	GGNNCC	2 cut(s)	346, 730
BmrFI	CCNGG	1 cut(s)	342
BmsI	GCATC	2 cut(s)	1226, 1238
BpiI	GAAGAC	2 cut(s)	155, 260
Bpu10I	CCTNAGC	1 cut(s)	106
BpuEI	CTTGAG	2 cut(s)	744, 759
BsaAI	YACGTR	2 cut(s)	324, 1250
BsaJI	CCNNGG	6 cut(s)	52, 173, 341, 391, 720, 1163
BsaWI	WCCGGW	1 cut(s)	347
BsaXI	ACNNNNNCTCC	2 cut(s)	139, 169
Bsc4I	CCNNNNNNNGG	3 cut(s)	347, 496, 941
Bse1I	ACTGG	1 cut(s)	739
Bse21I	CCTNAGG	1 cut(s)	381
BseAI	TCCGGA	1 cut(s)	347
BseBI	CCWGG	1 cut(s)	342
BseDI	CCNNGG	6 cut(s)	52, 173, 341, 391, 720, 1163
BseGI	GGATG	4 cut(s)	311, 442, 1217, 1229
BseLI	CCNNNNNNNGG	3 cut(s)	347, 496, 941
BseMII	CTCAG	4 cut(s)	120, 372, 625, 1075
BseNI	ACTGG	1 cut(s)	739
BseRI	GAGGAG	1 cut(s)	1185
BseXI	GCAGC	6 cut(s)	319, 586, 772, 1006, 1019, 1133
BsgI	GTGCAG	1 cut(s)	771
Bsh1236I	CGCG	1 cut(s)	695
BshFI	GGCC	5 cut(s)	396, 553, 719, 990, 1066
BsiHKAI	GWGCWC	1 cut(s)	50
BsiSI	CCGG	1 cut(s)	348
BslFI	GGGAC	2 cut(s)	113, 714
BslI	CCNNNNNNNGG	3 cut(s)	347, 496, 941
BsmFI	GGGAC	2 cut(s)	113, 714
BsmI	GAATGC	1 cut(s)	244
BsnI	GGCC	5 cut(s)	396, 553, 719, 990, 1066
Bsp1286I	GDGCHC	2 cut(s)	50, 612
Bsp13I	TCCGGA	1 cut(s)	347
Bsp143I	GATC	1 cut(s)	65
BspACI	CCGC	5 cut(s)	39, 256, 490, 554, 629
BspANI	GGCC	5 cut(s)	396, 553, 719, 990, 1066
BspCNI	CTCAG	4 cut(s)	119, 373, 624, 1074
BspEI	TCCGGA	1 cut(s)	347
BspFNI	CGCG	1 cut(s)	695
BspLI	GGNNCC	2 cut(s)	346, 730
BspMAI	CTGCAG	1 cut(s)	1123
BspPI	GGATC	1 cut(s)	73
BsrI	ACTGG	1 cut(s)	739
BssECI	CCNNGG	6 cut(s)	52, 173, 341, 391, 720, 1163
BssMI	GATC	1 cut(s)	65
BssSI	CACGAG	1 cut(s)	623
BssT1I	CCWWGG	4 cut(s)	52, 173, 391, 720
Bst2BI	CACGAG	1 cut(s)	623
Bst2UI	CCWGG	1 cut(s)	342
Bst4CI	ACNGT	3 cut(s)	11, 127, 266
Bst6I	CTCTTC	1 cut(s)	541
BstBAI	YACGTR	2 cut(s)	324, 1250
BstC8I	GCNNGC	1 cut(s)	879
BstDEI	CTNAG	5 cut(s)	106, 381, 526, 611, 1061
BstF5I	GGATG	4 cut(s)	311, 442, 1217, 1229
BstFNI	CGCG	1 cut(s)	695
BstH2I	RGCGCY	1 cut(s)	600
BstHHI	GCGC	1 cut(s)	599
BstKTI	GATC	1 cut(s)	68
BstMBI	GATC	1 cut(s)	65
BstMWI	GCNNNNNNNGC	3 cut(s)	596, 1016, 1028
BstNI	CCWGG	1 cut(s)	342
BstSCI	CCNGG	1 cut(s)	340
BstSFI	CTRYAG	3 cut(s)	804, 835, 1119
BstUI	CGCG	1 cut(s)	695
BstV1I	GCAGC	6 cut(s)	319, 586, 772, 1006, 1019, 1133
BstV2I	GAAGAC	2 cut(s)	155, 260
BstXI	CCANNNNNNTGG	1 cut(s)	567
Bsu36I	CCTNAGG	1 cut(s)	381
BsuI	GTATCC	1 cut(s)	967
BsuRI	GGCC	5 cut(s)	396, 553, 719, 990, 1066
BtsCI	GGATG	4 cut(s)	311, 442, 1217, 1229
BtsI	GCAGTG	1 cut(s)	157
BtsIMutI	CAGTG	3 cut(s)	157, 732, 1083
Cac8I	GCNNGC	1 cut(s)	879
CaiI	CAGNNNCTG	1 cut(s)	916
CfoI	GCGC	1 cut(s)	599
Cfr13I	GGNCC	4 cut(s)	345, 551, 728, 988
Csp6I	GTAC	4 cut(s)	469, 771, 851, 1247
CspCI	CAANNNNNGTGG	2 cut(s)	1059, 1094
CviAII	CATG	4 cut(s)	511, 567, 749, 766
CviQI	GTAC	4 cut(s)	469, 771, 851, 1247
DdeI	CTNAG	5 cut(s)	106, 381, 526, 611, 1061
DpnI	GATC	1 cut(s)	67
DpnII	GATC	1 cut(s)	65
DrdI	GACNNNNNNGTC	2 cut(s)	130, 751
DseDI	GACNNNNNNGTC	2 cut(s)	130, 751
EaeI	YGGCCR	1 cut(s)	717
Eam1104I	CTCTTC	1 cut(s)	541
EarI	CTCTTC	1 cut(s)	541
EciI	GGCGGA	2 cut(s)	245, 644
Ecl136II	GAGCTC	1 cut(s)	48
Eco130I	CCWWGG	4 cut(s)	52, 173, 391, 720
Eco147I	AGGCCT	1 cut(s)	1066
Eco24I	GRGCYC	2 cut(s)	50, 612
Eco47I	GGWCC	2 cut(s)	345, 728
Eco53kI	GAGCTC	1 cut(s)	48
Eco57I	CTGAAG	1 cut(s)	201
Eco72I	CACGTG	1 cut(s)	324
Eco81I	CCTNAGG	1 cut(s)	381
EcoICRI	GAGCTC	1 cut(s)	48
EcoRI	GAATTC	1 cut(s)	686
EcoRII	CCWGG	1 cut(s)	340
EcoT14I	CCWWGG	4 cut(s)	52, 173, 391, 720
EcoT22I	ATGCAT	1 cut(s)	1219
EcoT38I	GRGCYC	2 cut(s)	50, 612
ErhI	CCWWGG	4 cut(s)	52, 173, 391, 720
FaeI	CATG	4 cut(s)	514, 570, 752, 769
FaqI	GGGAC	2 cut(s)	113, 714
FatI	CATG	4 cut(s)	510, 566, 748, 765
FblI	GTMKAC	2 cut(s)	33, 755
Fnu4HI	GCNGC	7 cut(s)	333, 554, 600, 786, 1008, 1020, 1122
FokI	GGATG	4 cut(s)	318, 429, 1204, 1216
FriOI	GRGCYC	2 cut(s)	50, 612
Fsp4HI	GCNGC	7 cut(s)	333, 554, 600, 786, 1008, 1020, 1122
FspBI	CTAG	2 cut(s)	96, 677
GlaI	GCGC	1 cut(s)	598
GluI	GCNGC	7 cut(s)	333, 554, 600, 786, 1008, 1020, 1122
HaeII	RGCGCY	1 cut(s)	600
HaeIII	GGCC	5 cut(s)	396, 553, 719, 990, 1066
HapII	CCGG	1 cut(s)	348
HhaI	GCGC	1 cut(s)	599
Hin1II	CATG	4 cut(s)	514, 570, 752, 769
Hin6I	GCGC	1 cut(s)	597
HinP1I	GCGC	1 cut(s)	597
HincII	GTYRAC	4 cut(s)	34, 411, 434, 816
HindII	GTYRAC	4 cut(s)	34, 411, 434, 816
HindIII	AAGCTT	3 cut(s)	458, 523, 698
HinfI	GANTC	8 cut(s)	17, 532, 632, 680, 745, 913, 1052, 1106
HpaII	CCGG	1 cut(s)	348
HphI	GGTGA	4 cut(s)	287, 494, 749, 956
Hpy166II	GTNNAC	6 cut(s)	34, 327, 411, 434, 756, 816
Hpy188I	TCNGA	5 cut(s)	685, 706, 966, 1141, 1167
Hpy188III	TCNNGA	5 cut(s)	348, 367, 623, 650, 870
Hpy8I	GTNNAC	6 cut(s)	34, 327, 411, 434, 756, 816
HpyAV	CCTTC	6 cut(s)	111, 138, 176, 226, 272, 867
HpyCH4III	ACNGT	3 cut(s)	11, 127, 266
HpyCH4IV	ACGT	2 cut(s)	323, 1249
HpyCH4V	TGCA	6 cut(s)	335, 602, 788, 1047, 1121, 1217
HpyF10VI	GCNNNNNNNGC	3 cut(s)	596, 1016, 1028
HpyF3I	CTNAG	5 cut(s)	106, 381, 526, 611, 1061
HpySE526I	ACGT	2 cut(s)	323, 1249
Hsp92II	CATG	4 cut(s)	514, 570, 752, 769
HspAI	GCGC	1 cut(s)	597
Kpn2I	TCCGGA	1 cut(s)	347
Kzo9I	GATC	1 cut(s)	65
LmnI	GCTCC	4 cut(s)	53, 115, 147, 907
Lsp1109I	GCAGC	6 cut(s)	319, 586, 772, 1006, 1019, 1133
LweI	GCATC	2 cut(s)	1226, 1238
MaeI	CTAG	2 cut(s)	96, 677
MaeII	ACGT	2 cut(s)	323, 1249
MaeIII	GTNAC	1 cut(s)	737
MalI	GATC	1 cut(s)	67
MboI	GATC	1 cut(s)	65
MboII	GAAGA	5 cut(s)	12, 155, 260, 558, 611
MhlI	GDGCHC	2 cut(s)	50, 612
MlsI	TGGCCA	1 cut(s)	719
MluCI	AATT	3 cut(s)	205, 421, 686
MluNI	TGGCCA	1 cut(s)	719
MlyI	GAGTC	1 cut(s)	739
MmeI	TCCRAC	2 cut(s)	111, 963
Mox20I	TGGCCA	1 cut(s)	719
Mph1103I	ATGCAT	1 cut(s)	1219
MroI	TCCGGA	1 cut(s)	347
MroXI	GAANNNNTTC	1 cut(s)	215
MscI	TGGCCA	1 cut(s)	719
MseI	TTAA	4 cut(s)	312, 456, 657, 1258
MslI	CAYNNNNRTG	1 cut(s)	565
Msp20I	TGGCCA	1 cut(s)	719
MspA1I	CMGCKG	2 cut(s)	332, 1019
MspI	CCGG	1 cut(s)	348
MspR9I	CCNGG	1 cut(s)	342
Mva1269I	GAATGC	1 cut(s)	244
MvaI	CCWGG	1 cut(s)	342
MvnI	CGCG	1 cut(s)	695
MwoI	GCNNNNNNNGC	3 cut(s)	596, 1016, 1028
NdeII	GATC	1 cut(s)	65
NlaIII	CATG	4 cut(s)	514, 570, 752, 769
NlaIV	GGNNCC	2 cut(s)	346, 730
NmuCI	GTSAC	1 cut(s)	737
NsiI	ATGCAT	1 cut(s)	1219
PceI	AGGCCT	1 cut(s)	1066
PctI	GAATGC	1 cut(s)	244
PdmI	GAANNNNTTC	1 cut(s)	215
PfeI	GAWTC	7 cut(s)	17, 532, 632, 680, 913, 1052, 1106
PkrI	GCNGC	7 cut(s)	334, 555, 601, 787, 1009, 1021, 1123
PleI	GAGTC	1 cut(s)	739
PmaCI	CACGTG	1 cut(s)	324
PmlI	CACGTG	1 cut(s)	324
PpsI	GAGTC	1 cut(s)	739
Ppu21I	YACGTR	2 cut(s)	324, 1250
PshBI	ATTAAT	1 cut(s)	312
Psp124BI	GAGCTC	1 cut(s)	50
Psp6I	CCWGG	1 cut(s)	340
PspCI	CACGTG	1 cut(s)	324
PspGI	CCWGG	1 cut(s)	340
PspN4I	GGNNCC	2 cut(s)	346, 730
PspPI	GGNCC	4 cut(s)	345, 551, 728, 988
PstI	CTGCAG	1 cut(s)	1123
PstNI	CAGNNNCTG	1 cut(s)	916
PvuII	CAGCTG	2 cut(s)	332, 1019
RsaI	GTAC	4 cut(s)	470, 772, 852, 1248
RsaNI	GTAC	4 cut(s)	469, 771, 851, 1247
RseI	CAYNNNNRTG	1 cut(s)	565
SacI	GAGCTC	1 cut(s)	50
SalI	GTCGAC	1 cut(s)	32
SaqAI	TTAA	4 cut(s)	312, 456, 657, 1258
SatI	GCNGC	7 cut(s)	333, 554, 600, 786, 1008, 1020, 1122
Sau3AI	GATC	1 cut(s)	65
Sau96I	GGNCC	4 cut(s)	345, 551, 728, 988
SchI	GAGTC	1 cut(s)	739
ScrFI	CCNGG	1 cut(s)	342
SduI	GDGCHC	2 cut(s)	50, 612
SfaNI	GCATC	2 cut(s)	1226, 1238
SfcI	CTRYAG	3 cut(s)	804, 835, 1119
SinI	GGWCC	2 cut(s)	345, 728
SmiMI	CAYNNNNRTG	1 cut(s)	565
SmlI	CTYRAG	2 cut(s)	759, 774
SmoI	CTYRAG	2 cut(s)	759, 774
Sse9I	AATT	3 cut(s)	205, 421, 686
SseBI	AGGCCT	1 cut(s)	1066
SsiI	CCGC	5 cut(s)	39, 256, 490, 554, 629
SspI	AATATT	2 cut(s)	192, 670
SspMI	CTAG	2 cut(s)	96, 677
SstI	GAGCTC	1 cut(s)	50
StuI	AGGCCT	1 cut(s)	1066
StyD4I	CCNGG	1 cut(s)	340
StyI	CCWWGG	4 cut(s)	52, 173, 391, 720
TaaI	ACNGT	3 cut(s)	11, 127, 266
TaiI	ACGT	2 cut(s)	326, 1252
TaqI	TCGA	1 cut(s)	33
TasI	AATT	3 cut(s)	205, 421, 686
TatI	WGTACW	1 cut(s)	850
TauI	GCSGC	1 cut(s)	556
TfiI	GAWTC	7 cut(s)	17, 532, 632, 680, 913, 1052, 1106
Tru1I	TTAA	4 cut(s)	312, 456, 657, 1258
Tru9I	TTAA	4 cut(s)	312, 456, 657, 1258
TscAI	CASTG	3 cut(s)	157, 739, 1083
TseFI	GTSAC	1 cut(s)	737
TseI	GCWGC	6 cut(s)	332, 599, 785, 1007, 1019, 1121
Tsp45I	GTSAC	1 cut(s)	737
TspDTI	ATGAA	3 cut(s)	499, 555, 596
TspRI	CASTG	3 cut(s)	157, 739, 1083
VpaK11BI	GGWCC	2 cut(s)	345, 728
VspI	ATTAAT	1 cut(s)	312
XapI	RAATTY	2 cut(s)	205, 686
XmiI	GTMKAC	2 cut(s)	33, 755
XmnI	GAANNNNTTC	1 cut(s)	215
XspI	CTAG	2 cut(s)	96, 677
Zsp2I	ATGCAT	1 cut(s)	1219

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.