MD13G1076400.v1.1

ubiquitin-protein transferase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0017318
Physical Location & Seq
Forward (+)
5413649 .. 5414251
603 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1076400.v1.1.491

Sequence Viewer

Length: 603 bp
ATGTTGTTGTTGACTGGAGTCAGAATAAGACGGGAGGCGATTGTGAAGCTAATCATCAACGGTGGGCTCAAAATTCGCAATAAATATTGCTACAGGGGGCGTCAGGTATTAGGGTTGCACGTTTATGTCAGAAGTGAGTACCTCAGGGTCCGATGTAATTTCTGTACAGTAGGAAGGGGTACTCGCATGATGGAAGCAAGGTTAGCGCAATTGACGAACGATGATAATAGTAACAAGGGCATGGTGCCAGCGAGTTATCCAGCGATTAATAAAGTACTAAAGAGGGTTAGAGTGGGTAGTACTTCTGCGGATGATGGAGAATCGCCATCCCATGATGATGTGGAGGAGGAGGGTAGAGAGAGAAAAAGAAGGAAAATTAGGGTAAGTGAAAGTGAGAGTTGCACTGTTTGTATGGAACAGTTCAAGGGAGGATCAACGGTGGTTTGCATGCCTTGCTTTCATGTGTTTCATGGCAGGTGCATAGTGAAGTGGCTGAGAAAGAGTCAATATTGTCCGGTTTGCCGATTTCAAGTGCCTACTGATTCTAACCAAAAAGAAGTGCCTACAGATTCTGAACAAAAAAATGAAGTGCCTACTGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2

Protein Analysis

201

Amino Acids

22.83

Weight (kDa)

9.4

Isoelectric Point (pI)

51.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-rbx1 PF12678 129 - 175 3.4e-09 RING-H2 zinc finger domain
zf-RING_2 PF13639 133 - 175 8.6e-14 Ring finger domain
zf-C3HC4_2 PF13923 134 - 174 2.4e-10 Zinc finger, C3HC4 type (RING finger)
zf-RING_11 PF17123 134 - 161 3.1e-08 RING-like zinc finger
zf-C3HC4 PF00097 134 - 174 2.9e-06 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0017318)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g01430
malus_domestica MD13G1076400.v1.1
prunus_persica Prupe.1G270100_v2.0.a1 Prupe.1G270300_v2.0.a1 Prupe.2G025300_v2.0.a1
rosa_chinensis RchiOBHm_Chr3g0449061
rosa_laevigata RLG00000025857
rosa_multiflora Rmu_co8511853.1_g000002 Rmu_sc0003116.1_g000007
rosa_roxburghii Rroxscaffold_6G00427170
rosa_samantha Rh3BG016400 Rh3CG015400
rosa_wichuraiana Rw3G001220

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 465
Acc36I ACCTGC 1 cut(s) 465
AccB1I GGYRCC 1 cut(s) 244
AciI CCGC 1 cut(s) 308
AclWI GGATC 1 cut(s) 439
AcsI RAATTY 1 cut(s) 72
AcyI GRCGYC 1 cut(s) 100
AfaI GTAC 5 cut(s) 140, 166, 181, 276, 301
AgsI TTSAA 2 cut(s) 424, 530
AluBI AGCT 1 cut(s) 49
AluI AGCT 1 cut(s) 49
AlwI GGATC 1 cut(s) 439
AlwNI CAGNNNCTG 1 cut(s) 572
ApoI RAATTY 1 cut(s) 72
AseI ATTAAT 1 cut(s) 267
AspLEI GCGC 1 cut(s) 208
AspS9I GGNCC 1 cut(s) 148
AvaII GGWCC 1 cut(s) 148
AxyI CCTNAGG 1 cut(s) 143
BanI GGYRCC 1 cut(s) 244
BanII GRGCYC 1 cut(s) 69
BccI CCATC 3 cut(s) 184, 308, 334
BfmI CTRYAG 2 cut(s) 91, 564
BfuAI ACCTGC 1 cut(s) 465
BmcAI AGTACT 2 cut(s) 276, 301
Bme18I GGWCC 1 cut(s) 148
BmgT120I GGNCC 1 cut(s) 148
BmiI GGNNCC 2 cut(s) 149, 246
BoxI GACNNNNGTC 1 cut(s) 17
BpmI CTGGAG 1 cut(s) 36
BsaHI GRCGYC 1 cut(s) 100
BsaWI WCCGGW 1 cut(s) 514
Bse1I ACTGG 1 cut(s) 19
Bse21I CCTNAGG 1 cut(s) 143
BseGI GGATG 2 cut(s) 316, 326
BseMII CTCAG 2 cut(s) 157, 485
BseNI ACTGG 1 cut(s) 19
BseRI GAGGAG 2 cut(s) 359, 362
BshNI GGYRCC 1 cut(s) 244
BsiSI CCGG 1 cut(s) 515
Bsp1286I GDGCHC 1 cut(s) 69
Bsp1407I TGTACA 1 cut(s) 164
Bsp143I GATC 1 cut(s) 431
BspACI CCGC 1 cut(s) 308
BspCNI CTCAG 2 cut(s) 156, 486
BspLI GGNNCC 2 cut(s) 149, 246
BspMI ACCTGC 1 cut(s) 465
BspPI GGATC 1 cut(s) 439
BspT107I GGYRCC 1 cut(s) 244
BsrGI TGTACA 1 cut(s) 164
BsrI ACTGG 1 cut(s) 19
BssMI GATC 1 cut(s) 431
BssNI GRCGYC 1 cut(s) 100
Bst4CI ACNGT 5 cut(s) 62, 169, 406, 420, 439
BstACI GRCGYC 1 cut(s) 100
BstAPI GCANNNNNTGC 1 cut(s) 453
BstAUI TGTACA 1 cut(s) 164
BstC8I GCNNGC 2 cut(s) 249, 449
BstDEI CTNAG 2 cut(s) 143, 494
BstF5I GGATG 2 cut(s) 316, 326
BstHHI GCGC 1 cut(s) 208
BstKTI GATC 1 cut(s) 434
BstMBI GATC 1 cut(s) 431
BstMWI GCNNNNNNNGC 2 cut(s) 203, 453
BstNSI RCATGY 1 cut(s) 451
BstPAI GACNNNNGTC 1 cut(s) 17
BstSFI CTRYAG 2 cut(s) 91, 564
Bsu36I CCTNAGG 1 cut(s) 143
BtsCI GGATG 2 cut(s) 316, 326
BtsIMutI CAGTG 1 cut(s) 402
BveI ACCTGC 1 cut(s) 465
Cac8I GCNNGC 2 cut(s) 249, 449
CaiI CAGNNNCTG 1 cut(s) 572
CfoI GCGC 1 cut(s) 208
Cfr13I GGNCC 1 cut(s) 148
CseI GACGC 1 cut(s) 89
Csp6I GTAC 5 cut(s) 139, 165, 180, 275, 300
CviAII CATG 6 cut(s) 187, 241, 332, 448, 461, 470
CviJI RGCY 3 cut(s) 49, 67, 493
CviKI_1 RGCY 3 cut(s) 49, 67, 493
CviQI GTAC 5 cut(s) 139, 165, 180, 275, 300
DdeI CTNAG 2 cut(s) 143, 494
DpnI GATC 1 cut(s) 433
DpnII GATC 1 cut(s) 431
Eco24I GRGCYC 1 cut(s) 69
Eco47I GGWCC 1 cut(s) 148
Eco81I CCTNAGG 1 cut(s) 143
EcoT38I GRGCYC 1 cut(s) 69
FaeI CATG 6 cut(s) 190, 244, 335, 451, 464, 473
FaiI YATR 9 cut(s) 126, 188, 242, 333, 413, 449, 462, 471, 482
FatI CATG 6 cut(s) 186, 240, 331, 447, 460, 469
FokI GGATG 2 cut(s) 313, 323
FriOI GRGCYC 1 cut(s) 69
GlaI GCGC 1 cut(s) 207
GsuI CTGGAG 1 cut(s) 36
HapII CCGG 1 cut(s) 515
HgaI GACGC 1 cut(s) 89
HhaI GCGC 1 cut(s) 208
Hin1I GRCGYC 1 cut(s) 100
Hin1II CATG 6 cut(s) 190, 244, 335, 451, 464, 473
Hin6I GCGC 1 cut(s) 206
HinP1I GCGC 1 cut(s) 206
HincII GTYRAC 1 cut(s) 12
HindII GTYRAC 1 cut(s) 12
HinfI GANTC 5 cut(s) 18, 320, 502, 542, 569
HpaII CCGG 1 cut(s) 515
Hpy166II GTNNAC 1 cut(s) 12
Hpy188I TCNGA 4 cut(s) 23, 131, 152, 574
Hpy8I GTNNAC 1 cut(s) 12
HpyAV CCTTC 2 cut(s) 168, 363
HpyCH4III ACNGT 5 cut(s) 62, 169, 406, 420, 439
HpyCH4IV ACGT 1 cut(s) 120
HpyCH4V TGCA 4 cut(s) 118, 402, 447, 480
HpyF10VI GCNNNNNNNGC 2 cut(s) 203, 453
HpyF3I CTNAG 2 cut(s) 143, 494
HpySE526I ACGT 1 cut(s) 120
Hsp92I GRCGYC 1 cut(s) 100
Hsp92II CATG 6 cut(s) 190, 244, 335, 451, 464, 473
HspAI GCGC 1 cut(s) 206
Kzo9I GATC 1 cut(s) 431
LpnPI CCDG 7 cut(s) 79, 89, 130, 261, 273, 460, 528
MaeII ACGT 1 cut(s) 120
MaeIII GTNAC 1 cut(s) 230
MalI GATC 1 cut(s) 433
MboI GATC 1 cut(s) 431
MfeI CAATTG 1 cut(s) 209
MhlI GDGCHC 1 cut(s) 69
MluCI AATT 4 cut(s) 72, 157, 209, 375
MlyI GAGTC 2 cut(s) 27, 511
MnlI CCTC 7 cut(s) 28, 152, 276, 337, 340, 343, 422
MseI TTAA 1 cut(s) 267
MslI CAYNNNNRTG 2 cut(s) 123, 336
MspI CCGG 1 cut(s) 515
MunI CAATTG 1 cut(s) 209
MwoI GCNNNNNNNGC 2 cut(s) 203, 453
NdeII GATC 1 cut(s) 431
NlaIII CATG 6 cut(s) 190, 244, 335, 451, 464, 473
NlaIV GGNNCC 2 cut(s) 149, 246
NspI RCATGY 1 cut(s) 451
PaeI GCATGC 1 cut(s) 451
PaqCI CACCTGC 1 cut(s) 465
PfeI GAWTC 3 cut(s) 320, 542, 569
PleI GAGTC 2 cut(s) 26, 510
PpsI GAGTC 2 cut(s) 26, 510
PshAI GACNNNNGTC 1 cut(s) 17
PshBI ATTAAT 1 cut(s) 267
PspN4I GGNNCC 2 cut(s) 149, 246
PspPI GGNCC 1 cut(s) 148
PstNI CAGNNNCTG 1 cut(s) 572
RsaI GTAC 5 cut(s) 140, 166, 181, 276, 301
RsaNI GTAC 5 cut(s) 139, 165, 180, 275, 300
RseI CAYNNNNRTG 2 cut(s) 123, 336
SaqAI TTAA 1 cut(s) 267
Sau3AI GATC 1 cut(s) 431
Sau96I GGNCC 1 cut(s) 148
ScaI AGTACT 2 cut(s) 276, 301
SchI GAGTC 2 cut(s) 27, 511
SduI GDGCHC 1 cut(s) 69
SetI ASST 6 cut(s) 51, 108, 123, 144, 203, 479
SfcI CTRYAG 2 cut(s) 91, 564
SinI GGWCC 1 cut(s) 148
SmiMI CAYNNNNRTG 2 cut(s) 123, 336
SphI GCATGC 1 cut(s) 451
Sse9I AATT 4 cut(s) 72, 157, 209, 375
SsiI CCGC 1 cut(s) 308
SspI AATATT 2 cut(s) 86, 509
TaaI ACNGT 5 cut(s) 62, 169, 406, 420, 439
TaiI ACGT 1 cut(s) 123
TasI AATT 4 cut(s) 72, 157, 209, 375
TatI WGTACW 3 cut(s) 164, 274, 299
TfiI GAWTC 3 cut(s) 320, 542, 569
Tru1I TTAA 1 cut(s) 267
Tru9I TTAA 1 cut(s) 267
TscAI CASTG 1 cut(s) 409
TspDTI ATGAA 3 cut(s) 449, 458, 600
TspRI CASTG 1 cut(s) 409
VpaK11BI GGWCC 1 cut(s) 148
VspI ATTAAT 1 cut(s) 267
XapI RAATTY 1 cut(s) 72
XceI RCATGY 1 cut(s) 451
ZrmI AGTACT 2 cut(s) 276, 301
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.