Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr13

Orthogroup ID

OG0010823

Physical Location & Seq

Genomic Coordinates Reverse (-)

5769872 .. 5773789

Gene Structure 3918 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD13G1082500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2022 bp

ATGAAGCGAGGCAAGTTCGACTCGTTGCTGCCGCCGAGTCGACTCAGAATGATTCAGGTTTTTATTGGGGGAGTGATCGTCTACCTGCTCCTGATCAGCTTCGAAATCCCCCACGTTTTGAAATCCGGGTTTGAGTCTTCGGGTTTGGACGACGGGCTGTTCGAGACGCTGGAGGACGCATTTCCCAGAACGTTTTGGCTCGAGAGCGAGGAGGAAATGGGCGAGAGGGACGTCCCGAGTAGGCCGGCGGAGGACGCGTTTCGGGTCGCCGGCGGCTCGCCGTACCGGACCCCCCAGCTGCAAATGCGGGGGGTCAAGAAAGTTTCCGGCTTGGTGTTCGAGGACACTCTGTTCGGCGGCAATGTCAGTAAGGACCAAGTCTCCGAGCTTCATAGAACCGCCAAGAGCGCTTGGGCAGCTGGGAAGAAGCTCTGGGCGGAGCTCGAGGCCAGAAAGCTCGAGTCTGAACTGAAGACCAAGCCCGAGAACCGCTCTGAGCCGTGCCCGTACTCGTTTTTGCTGTCCGGGTCGGAATTCGAGTCCCGGAAACGGGTCATGGTTCTCCCCTGTGGGATGACTCTGGGGTCTCACATAACGGTGGTGGGCACGCCGAGGTGGGCCCACTCGGAGTACGATCCGAAGATTGCGGTGCTGAAGGAGGGAGACGAGGCGGTGATGGTCTCGCAGTTTATGATGGAGTTGCAGGGTTTGAAGAATGTGGAGGGGGAGGACCCGCCGAGGATACTGCATTTCAATCCGAGGTTGAAGGGGGATTGGAGTGGGAAGCCGGTGATCGAGCAGAACACTTGTTACCGGATGCAATGGGGCTCGGCGCTCCGCTGCGAGGGGTGGGGGTCCCTGGCTGATGAAGATACTGTTGATGGACAGGTGAAATGTGAGAAGTGGATTCGTGATGATGACAATAAGACGGAAGAATCAAAGGCCACATGGTGGTTGAACAGGCTGATTGGACGAACGAAGAAGGTGACCATAGACTGGCCATACCCTTTTGCAGAGGGAAAGCTGTTTGTTCTTACCGTAAGTGCTGGTTTGGAAGGTTTCCATATCAATGTCGATGGGAGGCATATCACTGCTTTCCCTTATCGCACGGGATTTGTTCTTGAGGATGCTACCGGACTCTCTGTAAATGGAGACATTGATGTGCACTCTGTGCTTGCAGCCTCCTTACCCACATCACATCCTAGTTTTTCACCCCAGATGCACCTTGAAATGGTTACTAAGTGGAAAGCTCCGTTTCTTCCCTATGGGCATGTAGAGTTGTTCATTGGCATTCTTTCTGCTGGGAACCATTTTGCAGAGCGTATGGCTGTGAGAAAGTCTTGGATGCAGCATAAATTAATCAAATCTTCGCGTGTTGTGGCTCGTTTTTTTGTAGCATTGCACGGAAGAAAGGAAGTGAATATGGAGCTAATGAAAGAAGTAGATTATTTTGGGGACATTGTTATAGTTCCTTATATGGATAACTATGATCTGGTAGTATTGAAGACTGTTGCAATCTGCGAATATGGGGTTCGCACGGTGCCTGCAAGGTATATCATGAAGTGCGATGATGACACATTTATCAGGGTGGATGCTGTGCTCAAGGAAGCACGGAAAGTTCGTGGATATAGAAGCTTCTATATTGGAAATATGAACTACCACCACAAGCCTCTTCGCCATGGTAAATGGGCAGTGACGTATGAGGAATGGCCAGAAGAAGATTATCCACCCTATGCAAATGGTCCAGGATACATAATCTCCTCTGACATAGCAAAGTCCGTCGTATCCGAGTTTGAAAAGCACAAGTTGCGATTGTTCAAGATGGAAGATGTGAGCATGGGAATGTGGGTAGAGCAGTTCAACAAATCCAAACCAGTGGAGTATGTGCATAGCGGGAAGTTCTGCCAGTTTGGGTGCATCAACGACTATTACACCGCGCATTACCAATCTCCGAGACAAATGATATGCATGTGGGACAAATTGCAACAACAAGGGAAACCCCAGTGCTGCAATATGCGATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005794 GO:0006029 GO:0006464 GO:0006486 GO:0006493 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0008378 GO:0009058 GO:0009059 GO:0009100 GO:0009101 GO:0009507 GO:0009536 GO:0009791 GO:0009987 GO:0010154 GO:0010191 GO:0010192 GO:0010214 GO:0010384 GO:0010404 GO:0010405 GO:0012505 GO:0016740 GO:0016757 GO:0016758 GO:0018193 GO:0018208 GO:0018258 GO:0019538 GO:0022414 GO:0032501 GO:0032502 GO:0034645 GO:0036211 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043413 GO:0044036 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0048316 GO:0048354 GO:0048359 GO:0048519 GO:0048608 GO:0048731 GO:0048831 GO:0048856 GO:0050789 GO:0050793 GO:0051093 GO:0051239 GO:0051241 GO:0061458 GO:0065007 GO:0070085 GO:0071554 GO:0071704 GO:1900055 GO:1900056 GO:1901135 GO:1901137 GO:1901564 GO:1901566 GO:1901576 GO:1905622 GO:1990714 GO:2000024 GO:2000026

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Gal-bind_lectin Galactosyl_T

Protein Analysis

674

Amino Acids

76.95

Weight (kDa)

6.86

Isoelectric Point (pI)

37.45

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Gal-bind_lectin	PF00337	185 - 392	1.8e-29	Galactoside-binding lectin
Galactosyl_T	PF01762	440 - 620	6.2e-33	Galactosyltransferase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010823)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G27120 AT1G27120
fragaria_vesca	FvH4_4g35520 FvH4_4g35520 FvH4_4g35520
malus_domestica	MD13G1082500.v1.1 MD16G1081600.v1.1
prunus_persica	Prupe.1G264500_v2.0.a1 Prupe.1G264500_v2.0.a1
pyrus_communis	pycom16g07040
rosa_chinensis	RchiOBHm_Chr4g0445171
rosa_laevigata	RLG00000005761
rosa_multiflora	Rmu_sc0000050.1_g000038
rosa_roxburghii	Rroxscaffold_5G00385680
rosa_rugosa	Rorug04G0359600
rosa_samantha	Rh4AG420400 Rh4BG432300 Rh4CG446900 Rh4DG428400
rosa_wichuraiana	Rw4G036050

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	583
AatII	GACGTC	1 cut(s)	234
Acc36I	ACCTGC	1 cut(s)	93
AccB1I	GGYRCC	1 cut(s)	1540
AccB7I	CCANNNNNTGG	2 cut(s)	951, 996
AccBSI	CCGCTC	1 cut(s)	492
AccI	GTMKAC	2 cut(s)	40, 81
AccII	CGCG	3 cut(s)	257, 1372, 1937
AclI	AACGTT	1 cut(s)	191
AclWI	GGATC	1 cut(s)	629
AcoI	YGGCCR	2 cut(s)	998, 1709
AcsI	RAATTY	1 cut(s)	533
AcuI	CTGAAG	2 cut(s)	491, 674
AcyI	GRCGYC	1 cut(s)	231
AdeI	CACNNNGTG	2 cut(s)	951, 1171
AfaI	GTAC	3 cut(s)	284, 509, 632
AfeI	AGCGCT	1 cut(s)	409
AfiI	CCNNNNNNNGG	6 cut(s)	549, 550, 844, 951, 996, 1231
AflIII	ACRYGT	1 cut(s)	255
AjnI	CCWGG	2 cut(s)	858, 1744
AloI	GAACNNNNNNTCC	2 cut(s)	335, 367
Alw21I	GWGCWC	3 cut(s)	444, 1167, 1602
Alw26I	GTCTC	7 cut(s)	158, 385, 591, 657, 685, 1146, 1948
Alw44I	GTGCAC	1 cut(s)	1163
AlwI	GGATC	1 cut(s)	629
Ama87I	CYCGRG	5 cut(s)	200, 235, 443, 458, 482
Aor51HI	AGCGCT	1 cut(s)	409
AoxI	GGCC	6 cut(s)	242, 447, 618, 942, 998, 1709
ApaI	GGGCCC	1 cut(s)	622
ApaLI	GTGCAC	1 cut(s)	1163
ApeKI	GCWGC	7 cut(s)	28, 298, 416, 840, 1178, 1348, 2007
ApoI	RAATTY	1 cut(s)	533
ArsI	GACNNNNNNTTYG	4 cut(s)	143, 175, 335, 367
AseI	ATTAAT	1 cut(s)	1358
AspLEI	GCGC	3 cut(s)	410, 835, 1939
AspS9I	GGNCC	7 cut(s)	288, 373, 618, 619, 730, 855, 1742
AsuC2I	CCSGG	3 cut(s)	127, 526, 544
AsuHPI	GGTGA	5 cut(s)	685, 802, 901, 997, 1203
AsuII	TTCGAA	1 cut(s)	102
AvaI	CYCGRG	5 cut(s)	200, 235, 443, 458, 482
AvaII	GGWCC	5 cut(s)	288, 373, 730, 855, 1742
BaeGI	GKGCMC	4 cut(s)	506, 608, 622, 1167
BalI	TGGCCA	2 cut(s)	1000, 1711
BanI	GGYRCC	1 cut(s)	1540
BanII	GRGCYC	3 cut(s)	444, 622, 830
BbsI	GAAGAC	3 cut(s)	129, 479, 1511
Bbv12I	GWGCWC	3 cut(s)	444, 1167, 1602
BbvI	GCAGC	7 cut(s)	15, 285, 428, 827, 1190, 1360, 1994
BccI	CCATC	5 cut(s)	670, 688, 875, 1070, 1816
BceAI	ACGGC	2 cut(s)	265, 484
BcgI	CGANNNNNNTGC	2 cut(s)	727, 761
BciT130I	CCWGG	2 cut(s)	860, 1746
BciVI	GTATCC	3 cut(s)	735, 1742, 1795
BclI	TGATCA	1 cut(s)	93
BcnI	CCSGG	3 cut(s)	127, 526, 544
BcoDI	GTCTC	7 cut(s)	158, 385, 591, 657, 685, 1146, 1948
BfaI	CTAG	1 cut(s)	1203
BfoI	RGCGCY	2 cut(s)	411, 836
BfuAI	ACCTGC	1 cut(s)	93
BfuI	GTATCC	3 cut(s)	735, 1742, 1795
Bme1390I	CCNGG	5 cut(s)	127, 526, 544, 860, 1746
Bme18I	GGWCC	5 cut(s)	288, 373, 730, 855, 1742
BmeT110I	CYCGRG	5 cut(s)	200, 235, 443, 458, 482
BmgT120I	GGNCC	7 cut(s)	288, 373, 618, 619, 730, 855, 1742
BmiI	GGNNCC	7 cut(s)	290, 620, 732, 856, 857, 1307, 1542
BmrFI	CCNGG	5 cut(s)	127, 526, 544, 860, 1746
BmrI	ACTGGG	1 cut(s)	1996
BmsI	GCATC	6 cut(s)	807, 1117, 1209, 1335, 1582, 1926
BmuI	ACTGGG	1 cut(s)	1996
BpiI	GAAGAC	3 cut(s)	129, 479, 1511
BplI	GAGNNNNNCTC	2 cut(s)	476, 508
BpmI	CTGGAG	1 cut(s)	191
Bpu14I	TTCGAA	1 cut(s)	102
BpuEI	CTTGAG	2 cut(s)	1142, 1586
BpuMI	CCSGG	3 cut(s)	127, 526, 544
BsaHI	GRCGYC	1 cut(s)	231
BsaI	GGTCTC	2 cut(s)	591, 685
BsaJI	CCNNGG	5 cut(s)	611, 737, 758, 858, 1678
BsaWI	WCCGGW	3 cut(s)	285, 813, 1133
BsaXI	ACNNNNNCTCC	8 cut(s)	63, 93, 242, 272, 365, 395, 1742, 1772
Bsc4I	CCNNNNNNNGG	6 cut(s)	549, 550, 844, 951, 996, 1231
Bse118I	RCCGGY	3 cut(s)	244, 269, 787
Bse1I	ACTGG	4 cut(s)	1001, 1874, 1906, 2002
Bse3DI	GCAATG	3 cut(s)	367, 827, 1397
BseBI	CCWGG	2 cut(s)	860, 1746
BseDI	CCNNGG	5 cut(s)	611, 737, 758, 858, 1678
BseGI	GGATG	6 cut(s)	579, 822, 1132, 1198, 1350, 1597
BseLI	CCNNNNNNNGG	6 cut(s)	549, 550, 844, 951, 996, 1231
BseMI	GCAATG	3 cut(s)	367, 827, 1397
BseMII	CTCAG	2 cut(s)	58, 486
BseNI	ACTGG	4 cut(s)	1001, 1874, 1906, 2002
BseRI	GAGGAG	2 cut(s)	224, 1750
BseSI	GKGCMC	4 cut(s)	506, 608, 622, 1167
BseXI	GCAGC	7 cut(s)	15, 285, 428, 827, 1190, 1360, 1994
BseYI	CCCAGC	3 cut(s)	294, 419, 1301
Bsh1236I	CGCG	3 cut(s)	257, 1372, 1937
BshFI	GGCC	6 cut(s)	244, 449, 620, 944, 1000, 1711
BshNI	GGYRCC	1 cut(s)	1540
BsiHKAI	GWGCWC	3 cut(s)	444, 1167, 1602
BsiHKCI	CYCGRG	5 cut(s)	200, 235, 443, 458, 482
BslFI	GGGAC	6 cut(s)	218, 242, 526, 841, 1469, 1988
BslI	CCNNNNNNNGG	6 cut(s)	549, 550, 844, 951, 996, 1231
BsmAI	GTCTC	7 cut(s)	158, 385, 591, 657, 685, 1146, 1948
BsmBI	CGTCTC	2 cut(s)	158, 657
BsmFI	GGGAC	6 cut(s)	218, 242, 526, 841, 1469, 1988
BsmI	GAATGC	1 cut(s)	1290
BsnI	GGCC	6 cut(s)	244, 449, 620, 944, 1000, 1711
Bso31I	GGTCTC	2 cut(s)	591, 685
BsoBI	CYCGRG	5 cut(s)	200, 235, 443, 458, 482
Bsp119I	TTCGAA	1 cut(s)	102
Bsp120I	GGGCCC	1 cut(s)	618
Bsp1286I	GDGCHC	7 cut(s)	444, 506, 608, 622, 830, 1167, 1602
Bsp143I	GATC	5 cut(s)	75, 93, 634, 792, 1489
Bsp19I	CCATGG	1 cut(s)	1678
BspANI	GGCC	6 cut(s)	244, 449, 620, 944, 1000, 1711
BspCNI	CTCAG	2 cut(s)	57, 487
BspFNI	CGCG	3 cut(s)	257, 1372, 1937
BspHI	TCATGA	1 cut(s)	1557
BspLI	GGNNCC	7 cut(s)	290, 620, 732, 856, 857, 1307, 1542
BspMI	ACCTGC	1 cut(s)	93
BspPI	GGATC	1 cut(s)	629
BspT104I	TTCGAA	1 cut(s)	102
BspT107I	GGYRCC	1 cut(s)	1540
BspTNI	GGTCTC	2 cut(s)	591, 685
BsrBI	CCGCTC	1 cut(s)	492
BsrDI	GCAATG	3 cut(s)	367, 827, 1397
BsrFI	RCCGGY	3 cut(s)	244, 269, 787
BsrI	ACTGG	4 cut(s)	1001, 1874, 1906, 2002
BssAI	RCCGGY	3 cut(s)	244, 269, 787
BssECI	CCNNGG	5 cut(s)	611, 737, 758, 858, 1678
BssMI	GATC	5 cut(s)	75, 93, 634, 792, 1489
BssNI	GRCGYC	1 cut(s)	231
BssT1I	CCWWGG	1 cut(s)	1678
Bst2UI	CCWGG	2 cut(s)	860, 1746
Bst4CI	ACNGT	5 cut(s)	598, 877, 1039, 1510, 1540
Bst6I	CTCTTC	1 cut(s)	1677
BstACI	GRCGYC	1 cut(s)	231
BstAPI	GCANNNNNTGC	2 cut(s)	1171, 1807
BstBI	TTCGAA	1 cut(s)	102
BstC8I	GCNNGC	6 cut(s)	246, 271, 278, 608, 1176, 1545
BstDEI	CTNAG	3 cut(s)	44, 495, 1239
BstDSI	CCRYGG	1 cut(s)	1678
BstEII	GGTNACC	1 cut(s)	985
BstF5I	GGATG	6 cut(s)	579, 822, 1132, 1198, 1350, 1597
BstFNI	CGCG	3 cut(s)	257, 1372, 1937
BstH2I	RGCGCY	2 cut(s)	411, 836
BstHHI	GCGC	3 cut(s)	410, 835, 1939
BstKTI	GATC	5 cut(s)	78, 96, 637, 795, 1492
BstMAI	GTCTC	7 cut(s)	158, 385, 591, 657, 685, 1146, 1948
BstMBI	GATC	5 cut(s)	75, 93, 634, 792, 1489
BstMWI	GCNNNNNNNGC	6 cut(s)	254, 304, 407, 416, 1171, 1807
BstNI	CCWGG	2 cut(s)	860, 1746
BstNSI	RCATGY	2 cut(s)	1274, 1972
BstPI	GGTNACC	1 cut(s)	985
BstSCI	CCNGG	5 cut(s)	125, 524, 542, 858, 1744
BstSLI	GKGCMC	4 cut(s)	506, 608, 622, 1167
BstUI	CGCG	3 cut(s)	257, 1372, 1937
BstV1I	GCAGC	7 cut(s)	15, 285, 428, 827, 1190, 1360, 1994
BstV2I	GAAGAC	3 cut(s)	129, 479, 1511
BstXI	CCANNNNNNTGG	1 cut(s)	1876
BsuI	GTATCC	3 cut(s)	735, 1742, 1795
BsuRI	GGCC	6 cut(s)	244, 449, 620, 944, 1000, 1711
BtgI	CCRYGG	1 cut(s)	1678
BtgZI	GCGATG	1 cut(s)	1581
BtsCI	GGATG	6 cut(s)	579, 822, 1132, 1198, 1350, 1597
BtsI	GCAGTG	2 cut(s)	1089, 1698
BtsIMutI	CAGTG	4 cut(s)	1089, 1698, 1881, 2009
BveI	ACCTGC	1 cut(s)	93
Cac8I	GCNNGC	6 cut(s)	246, 271, 278, 608, 1176, 1545
CciI	TCATGA	1 cut(s)	1557
CfoI	GCGC	3 cut(s)	410, 835, 1939
Cfr10I	RCCGGY	3 cut(s)	244, 269, 787
Cfr13I	GGNCC	7 cut(s)	288, 373, 618, 619, 730, 855, 1742
CseI	GACGC	3 cut(s)	175, 185, 263
Csp6I	GTAC	3 cut(s)	283, 508, 631
CviAII	CATG	7 cut(s)	556, 948, 1271, 1558, 1679, 1837, 1969
CviQI	GTAC	3 cut(s)	283, 508, 631
DdeI	CTNAG	3 cut(s)	44, 495, 1239
DpnI	GATC	5 cut(s)	77, 95, 636, 794, 1491
DpnII	GATC	5 cut(s)	75, 93, 634, 792, 1489
DraIII	CACNNNGTG	2 cut(s)	951, 1171
DrdI	GACNNNNNNGTC	1 cut(s)	583
DseDI	GACNNNNNNGTC	1 cut(s)	583
EaeI	YGGCCR	2 cut(s)	998, 1709
Eam1104I	CTCTTC	1 cut(s)	1677
EarI	CTCTTC	1 cut(s)	1677
EciI	GGCGGA	2 cut(s)	263, 452
Ecl136II	GAGCTC	1 cut(s)	442
Eco130I	CCWWGG	1 cut(s)	1678
Eco24I	GRGCYC	3 cut(s)	444, 622, 830
Eco31I	GGTCTC	2 cut(s)	591, 685
Eco47I	GGWCC	5 cut(s)	288, 373, 730, 855, 1742
Eco47III	AGCGCT	1 cut(s)	409
Eco53kI	GAGCTC	1 cut(s)	442
Eco57I	CTGAAG	2 cut(s)	491, 674
Eco88I	CYCGRG	5 cut(s)	200, 235, 443, 458, 482
Eco91I	GGTNACC	1 cut(s)	985
EcoICRI	GAGCTC	1 cut(s)	442
EcoO109I	RGGNCCY	2 cut(s)	730, 855
EcoO65I	GGTNACC	1 cut(s)	985
EcoRI	GAATTC	1 cut(s)	533
EcoRII	CCWGG	2 cut(s)	858, 1744
EcoT14I	CCWWGG	1 cut(s)	1678
EcoT22I	ATGCAT	1 cut(s)	1970
EcoT38I	GRGCYC	3 cut(s)	444, 622, 830
ErhI	CCWWGG	1 cut(s)	1678
Esp3I	CGTCTC	2 cut(s)	158, 657
FaeI	CATG	7 cut(s)	559, 951, 1274, 1561, 1682, 1840, 1972
FaqI	GGGAC	6 cut(s)	218, 242, 526, 841, 1469, 1988
FatI	CATG	7 cut(s)	555, 947, 1270, 1557, 1678, 1836, 1968
FauI	CCCGC	3 cut(s)	300, 741, 1886
FbaI	TGATCA	1 cut(s)	93
FblI	GTMKAC	2 cut(s)	40, 81
FokI	GGATG	6 cut(s)	586, 829, 1139, 1185, 1357, 1604
FriOI	GRGCYC	3 cut(s)	444, 622, 830
FspBI	CTAG	1 cut(s)	1203
GlaI	GCGC	3 cut(s)	409, 834, 1938
GsaI	CCCAGC	3 cut(s)	298, 423, 1305
GsuI	CTGGAG	1 cut(s)	191
HaeII	RGCGCY	2 cut(s)	411, 836
HaeIII	GGCC	6 cut(s)	244, 449, 620, 944, 1000, 1711
HgaI	GACGC	3 cut(s)	175, 185, 263
HhaI	GCGC	3 cut(s)	410, 835, 1939
Hin1I	GRCGYC	1 cut(s)	231
Hin1II	CATG	7 cut(s)	559, 951, 1274, 1561, 1682, 1840, 1972
Hin6I	GCGC	3 cut(s)	408, 833, 1937
HinP1I	GCGC	3 cut(s)	408, 833, 1937
HincII	GTYRAC	1 cut(s)	41
HindII	GTYRAC	1 cut(s)	41
HindIII	AAGCTT	1 cut(s)	1633
HphI	GGTGA	5 cut(s)	685, 802, 901, 997, 1203
Hpy166II	GTNNAC	3 cut(s)	41, 82, 1165
Hpy188III	TCNNGA	9 cut(s)	91, 163, 202, 235, 316, 911, 1121, 1558, 1819
Hpy8I	GTNNAC	3 cut(s)	41, 82, 1165
Hpy99I	CGWCG	2 cut(s)	155, 1784
HpyAV	CCTTC	4 cut(s)	649, 760, 976, 1049
HpyCH4III	ACNGT	5 cut(s)	598, 877, 1039, 1510, 1540
HpyCH4IV	ACGT	4 cut(s)	114, 191, 231, 1697
HpyF10VI	GCNNNNNNNGC	6 cut(s)	254, 304, 407, 416, 1171, 1807
HpyF3I	CTNAG	3 cut(s)	44, 495, 1239
HpySE526I	ACGT	4 cut(s)	114, 191, 231, 1697
Hsp92I	GRCGYC	1 cut(s)	231
Hsp92II	CATG	7 cut(s)	559, 951, 1274, 1561, 1682, 1840, 1972
HspAI	GCGC	3 cut(s)	408, 833, 1937
KflI	GGGWCCC	1 cut(s)	855
KroI	GCCGGC	2 cut(s)	244, 269
KroNI	GCCGGC	2 cut(s)	246, 271
Ksp22I	TGATCA	1 cut(s)	93
Kzo9I	GATC	5 cut(s)	75, 93, 634, 792, 1489
LmnI	GCTCC	5 cut(s)	93, 439, 840, 1255, 1426
Lsp1109I	GCAGC	7 cut(s)	15, 285, 428, 827, 1190, 1360, 1994
LweI	GCATC	6 cut(s)	807, 1117, 1209, 1335, 1582, 1926
MaeI	CTAG	1 cut(s)	1203
MaeII	ACGT	4 cut(s)	114, 191, 231, 1697
MaeIII	GTNAC	4 cut(s)	809, 985, 1234, 1693
MalI	GATC	5 cut(s)	77, 95, 636, 794, 1491
MbiI	CCGCTC	1 cut(s)	492
MboI	GATC	5 cut(s)	75, 93, 634, 792, 1489
MhlI	GDGCHC	7 cut(s)	444, 506, 608, 622, 830, 1167, 1602
MlsI	TGGCCA	2 cut(s)	1000, 1711
MluCI	AATT	3 cut(s)	533, 1355, 1979
MluI	ACGCGT	1 cut(s)	255
MluNI	TGGCCA	2 cut(s)	1000, 1711
MlyI	GAGTC	8 cut(s)	14, 36, 46, 143, 470, 548, 571, 1131
MmeI	TCCRAC	1 cut(s)	510
Mox20I	TGGCCA	2 cut(s)	1000, 1711
Mph1103I	ATGCAT	1 cut(s)	1970
MreI	CGCCGGCG	1 cut(s)	269
MroNI	GCCGGC	2 cut(s)	244, 269
MscI	TGGCCA	2 cut(s)	1000, 1711
MseI	TTAA	1 cut(s)	1358
MslI	CAYNNNNRTG	4 cut(s)	596, 1068, 1160, 1841
Msp20I	TGGCCA	2 cut(s)	1000, 1711
MspA1I	CMGCKG	3 cut(s)	298, 419, 840
MspR9I	CCNGG	5 cut(s)	127, 526, 544, 860, 1746
Mva1269I	GAATGC	1 cut(s)	1290
MvaI	CCWGG	2 cut(s)	860, 1746
MvnI	CGCG	3 cut(s)	257, 1372, 1937
MwoI	GCNNNNNNNGC	6 cut(s)	254, 304, 407, 416, 1171, 1807
NaeI	GCCGGC	2 cut(s)	246, 271
NciI	CCSGG	3 cut(s)	127, 526, 544
NcoI	CCATGG	1 cut(s)	1678
NdeII	GATC	5 cut(s)	75, 93, 634, 792, 1489
NgoMIV	GCCGGC	2 cut(s)	244, 269
NlaIII	CATG	7 cut(s)	559, 951, 1274, 1561, 1682, 1840, 1972
NlaIV	GGNNCC	7 cut(s)	290, 620, 732, 856, 857, 1307, 1542
NmeAIII	GCCGAG	4 cut(s)	60, 636, 762, 809
NmuCI	GTSAC	2 cut(s)	985, 1693
NsiI	ATGCAT	1 cut(s)	1970
NspI	RCATGY	2 cut(s)	1274, 1972
NspV	TTCGAA	1 cut(s)	102
PaeR7I	CTCGAG	3 cut(s)	200, 443, 458
PagI	TCATGA	1 cut(s)	1557
PcsI	WCGNNNNNNNCGW	2 cut(s)	159, 1618
PctI	GAATGC	1 cut(s)	1290
PdiI	GCCGGC	2 cut(s)	246, 271
PfeI	GAWTC	3 cut(s)	52, 907, 935
PflFI	GACNNNGTC	1 cut(s)	377
PflMI	CCANNNNNTGG	2 cut(s)	951, 996
PfoI	TCCNGGA	2 cut(s)	542, 1744
PleI	GAGTC	8 cut(s)	14, 36, 45, 142, 469, 547, 571, 1131
PpsI	GAGTC	8 cut(s)	14, 36, 45, 142, 469, 547, 571, 1131
PpuMI	RGGWCCY	2 cut(s)	730, 855
PshBI	ATTAAT	1 cut(s)	1358
Psp124BI	GAGCTC	1 cut(s)	444
Psp1406I	AACGTT	1 cut(s)	191
Psp5II	RGGWCCY	2 cut(s)	730, 855
Psp6I	CCWGG	2 cut(s)	858, 1744
PspEI	GGTNACC	1 cut(s)	985
PspFI	CCCAGC	3 cut(s)	294, 419, 1301
PspGI	CCWGG	2 cut(s)	858, 1744
PspN4I	GGNNCC	7 cut(s)	290, 620, 732, 856, 857, 1307, 1542
PspOMI	GGGCCC	1 cut(s)	618
PspPI	GGNCC	7 cut(s)	288, 373, 618, 619, 730, 855, 1742
PspPPI	RGGWCCY	2 cut(s)	730, 855
PspXI	VCTCGAGB	2 cut(s)	443, 458
PsrI	GAACNNNNNNTAC	2 cut(s)	794, 826
PsyI	GACNNNGTC	1 cut(s)	377
PvuII	CAGCTG	2 cut(s)	298, 419
RsaI	GTAC	3 cut(s)	284, 509, 632
RsaNI	GTAC	3 cut(s)	283, 508, 631
RseI	CAYNNNNRTG	4 cut(s)	596, 1068, 1160, 1841
SacI	GAGCTC	1 cut(s)	444
SalI	GTCGAC	1 cut(s)	39
SaqAI	TTAA	1 cut(s)	1358
Sau3AI	GATC	5 cut(s)	75, 93, 634, 792, 1489
Sau96I	GGNCC	7 cut(s)	288, 373, 618, 619, 730, 855, 1742
SchI	GAGTC	8 cut(s)	14, 36, 46, 143, 470, 548, 571, 1131
ScrFI	CCNGG	5 cut(s)	127, 526, 544, 860, 1746
SduI	GDGCHC	7 cut(s)	444, 506, 608, 622, 830, 1167, 1602
SfaNI	GCATC	6 cut(s)	807, 1117, 1209, 1335, 1582, 1926
Sfr274I	CTCGAG	3 cut(s)	200, 443, 458
SfuI	TTCGAA	1 cut(s)	102
SgrAI	CRCCGGYG	1 cut(s)	269
SinI	GGWCC	5 cut(s)	288, 373, 730, 855, 1742
SlaI	CTCGAG	3 cut(s)	200, 443, 458
SmiMI	CAYNNNNRTG	4 cut(s)	596, 1068, 1160, 1841
SmlI	CTYRAG	5 cut(s)	200, 443, 458, 1121, 1601
SmoI	CTYRAG	5 cut(s)	200, 443, 458, 1121, 1601
Sse9I	AATT	3 cut(s)	533, 1355, 1979
SspMI	CTAG	1 cut(s)	1203
SstI	GAGCTC	1 cut(s)	444
StyD4I	CCNGG	5 cut(s)	125, 524, 542, 858, 1744
StyI	CCWWGG	1 cut(s)	1678
TaaI	ACNGT	5 cut(s)	598, 877, 1039, 1510, 1540
TaiI	ACGT	4 cut(s)	117, 194, 234, 1700
TasI	AATT	3 cut(s)	533, 1355, 1979
TauI	GCSGC	3 cut(s)	34, 276, 360
TfiI	GAWTC	3 cut(s)	52, 907, 935
Tru1I	TTAA	1 cut(s)	1358
Tru9I	TTAA	1 cut(s)	1358
TscAI	CASTG	4 cut(s)	1096, 1698, 1881, 2009
TseFI	GTSAC	2 cut(s)	985, 1693
TseI	GCWGC	7 cut(s)	28, 298, 416, 840, 1178, 1348, 2007
Tsp45I	GTSAC	2 cut(s)	985, 1693
TspDTI	ATGAA	7 cut(s)	17, 380, 882, 1273, 1448, 1574, 1667
TspGWI	ACGGA	5 cut(s)	944, 1242, 1419, 1627, 1768
TspRI	CASTG	4 cut(s)	1096, 1698, 1881, 2009
Tth111I	GACNNNGTC	1 cut(s)	377
Van91I	CCANNNNNTGG	2 cut(s)	951, 996
VneI	GTGCAC	1 cut(s)	1163
VpaK11BI	GGWCC	5 cut(s)	288, 373, 730, 855, 1742
VspI	ATTAAT	1 cut(s)	1358
XapI	RAATTY	1 cut(s)	533
XceI	RCATGY	2 cut(s)	1274, 1972
XhoI	CTCGAG	3 cut(s)	200, 443, 458
XmiI	GTMKAC	2 cut(s)	40, 81
XspI	CTAG	1 cut(s)	1203
ZraI	GACGTC	1 cut(s)	232
Zsp2I	ATGCAT	1 cut(s)	1970

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.