Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr13

Orthogroup ID

OG0006595

Physical Location & Seq

Genomic Coordinates Reverse (-)

6771736 .. 6782546

Gene Structure 10811 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD13G1096000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2070 bp

ATGCATAAACAGAAGGTACCAATCCTTATTTCTTCCACGACATTCCTTCATATAAATTTGGATGGAGGCAGCTGCAAATTAAATCAAACTTCCAATTCATCCCAAAAAAAAATGAAGAAATGCAACGTTTCAATTCTTTCCCAACATTTGCTTCCGCTGAAGATCCTGCTGTTACTAGGAATGGCTGTCGGAGATCCAAGAACGAAGACGGTCCAAATAATGTGCGCCAACCAACGTGAGCACAACACAACCGTGTTTGTTCCGAATTTTGTCAGCAATATGGCAAACATCAGTGAACAAATGCAAACTTCCGGCTTCGGAGTGGCAGTCACCGGTTCAGGCCCCGACACCAACTACGGCCTAGCTCAATGCTATGGAGACCTTTCTCTTCTAGACTGTGTGCTATGCTACGCCGAGGCGCGTACAGTGCTTCCCCAATGTTTTCCTCACACTGGGGGTCGGATTTTCCTCGACGGTTGCTTCATGAGGTCTGAAAACTATAGCTTTTACGAGGAGTTTAGAGGATCGGAAGATAGTGTTGAGTGTGGGAACACCACGAGGAAGAGTTCGGCGTTCGGAGCCTCGGCAAGGCAGGTTGTGAAGCGTGCGGTTGAGTCTGCGCCAAGCAACCGAGGATATGCAAGGGGTGAGGTGAGCGTGAGTGGGATGGCAAACGAGTCAGCTTATGTGTTGGCTGATTGCTGGAGAACTTTGGATGAGAGCTCTTGCAGACAGTGTTTGGAAAATGCATCTGCATCAATGTTGGGATGCTTGCCTTGGTCCGAGGGGCGAGCGTTGTACACCGGGTGCTTTATGAGGTATTCAGACAGAGATTTTCTCAACAAGGAAATGGGAAATGGAAGCTCCAGAGGTACCACTATTGTCATAGTAGTTGCAGTTGTCAGCTCCTTGGTAGTTTTGGTAGTTGGAGTAGCCATCGGATTTTATATCTGGAAGCACAGATATATACAAAAGAAAAGAAGAGGATCATATGATGCAGAGAAGTGGGCGAAAACCCTAAACGACAGTAGCTTAAACTTCAAGTACTCAACAATTGAAAAGGCCACGGGGTCTTTTGACATTGTCAACAAGCTTGGACAAGGAGGTTTCGGAACTGTTTATAAGGGAGTTCTGGCTGATGGGAGAGAGATTGCAGTGAAGAGGCTTTTCTTCAACAACAGACACAGAGCAGCAGATTTCTACAATGAAATCAACATTATAAGCAGCGTGGAACACAGAAACTTGGTCAGGTTGTTAGGCTGCAGTTGCTCGGGACCTGAAAGCCTTCTCGTCTATGAATACCTACCGAACAGGAGTCTTGACCGCTTCATCTTCGATCAAGAAAGAGCTAAAACACTTAACTGGGAGAGGAGATATGATATCATTGTCGGAACAGCAGAAGGTTTAGCCTACCTTCACGACAACTCAAAAACAAGGATCATTCACAGAGACATAAAAGCGAGCAATATACTTTTGGATTCAAGGCTTCGTGCTAAAATTGCAGATTTTGGTTTGGCCAGGTCTTTCGAGGAAGATAAGAGTCACATCAGCACAGCAATTGCAGGAACATTGGGATACATGGCTCCAGAATACCTAGCTCATGGCCAGTTGACAGAAAAGGCAGATGTCTATAGCTTCGGAGTGCTTATATTGGAGATCATCACTGGAAGGCAGAACAACAGGAGCAAATCTGCAGAGTACTCAGATGGCATAGTTACAATTACCTGGAAGCACTTTCAAGCAGGGACTGTAGAGGAACTGTACGACCCAAATTTAATGTTGCAGAATTGCCATGACAATGTTAAGGATGAGATATTCAGAGTGGTTCAGATAGGACTTCTATGCACCCAAGAGAGTTCCTCTTTACGACCAACAATGTCAAAGGCGCTGCAGATGCTAACAAAGAAGGAGCTGCACCTACCTGCGCCCACCAATCCACCTTTTATAGATGAAAAGACCATGGAACTCAATGACCACGGCGACGACCAATGTTACCCTCTAAATGCAGATGGTGCTTCTTCAGTTGCGAGTGTTTCACATAGTTCTTTCTATCCAAGGGCTTGCAACTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000302 GO:0001101 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005623 GO:0005886 GO:0005911 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006979 GO:0008150 GO:0008152 GO:0009506 GO:0009987 GO:0010035 GO:0010193 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0030054 GO:0036211 GO:0042221 GO:0042493 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0046677 GO:0046777 GO:0050896 GO:0055044 GO:0071704 GO:0071944 GO:0140096 GO:1901564 GO:1901700

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Pkinase Pkinase_Tyr Stress-antifung

Protein Analysis

690

Amino Acids

76.57

Weight (kDa)

8.13

Isoelectric Point (pI)

42.88

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Stress-antifung	PF01657	75 - 167	1.5e-12	Salt stress response/antifungal
Stress-antifung	PF01657	182 - 276	2.3e-11	Salt stress response/antifungal
Pkinase	PF00069	360 - 631	2.4e-47	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	361 - 631	1.4e-47	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006595)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G70520 AT4G28670
fragaria_vesca	FvH4_4g26071 FvH4_4g26071 FvH4_4g26071
malus_domestica	MD13G1096000.v1.1 MD16G1097400.v1.1
prunus_persica	Prupe.1G249400_v2.0.a1 Prupe.1G249400_v2.0.a1 Prupe.1G249400_v2.0.a1
pyrus_communis	pycom13g08370 pycom13g08380 pycom13g08410
rosa_chinensis	RchiOBHm_Chr4g0433531
rosa_laevigata	RLG00000006745
rosa_multiflora	Rmu_sc0000312.1_g000009
rosa_roxburghii	Rroxscaffold_5G00375050
rosa_rugosa	Rorug04G0269400 Rorug04G0269500 Rorug04G0269600
rosa_samantha	Rh4AG324200 Rh4BG332200 Rh4CG347500 Rh4DG328500
rosa_wichuraiana	Rw0G020170 Rw4G028080

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	1122, 1220
Acc36I	ACCTGC	2 cut(s)	583, 1930
Acc65I	GGTACC	2 cut(s)	16, 872
AccB1I	GGYRCC	2 cut(s)	16, 872
AccII	CGCG	1 cut(s)	421
AciI	CCGC	3 cut(s)	155, 608, 1324
AclI	AACGTT	1 cut(s)	126
AclWI	GGATC	5 cut(s)	157, 188, 532, 994, 1445
AcoI	YGGCCR	2 cut(s)	1515, 1603
AcsI	RAATTY	3 cut(s)	55, 265, 1771
AcuI	CTGAAG	2 cut(s)	179, 2004
AdeI	CACNNNGTG	1 cut(s)	807
AfaI	GTAC	7 cut(s)	18, 424, 800, 874, 1046, 1700, 1763
AfiI	CCNNNNNNNGG	2 cut(s)	452, 588
AgeI	ACCGGT	1 cut(s)	332
AgsI	TTSAA	6 cut(s)	132, 1042, 1058, 1174, 1482, 1739
AjnI	CCWGG	2 cut(s)	1517, 1724
AjuI	GAANNNNNNNTTGG	4 cut(s)	13, 45, 135, 167
AleI	CACNNNNGTG	1 cut(s)	251
Alw21I	GWGCWC	2 cut(s)	243, 725
Alw26I	GTCTC	2 cut(s)	372, 1443
AlwI	GGATC	5 cut(s)	157, 188, 532, 994, 1445
AlwNI	CAGNNNCTG	1 cut(s)	1748
Ama87I	CYCGRG	1 cut(s)	1270
AoxI	GGCC	5 cut(s)	340, 358, 1062, 1515, 1603
ApeKI	GCWGC	7 cut(s)	69, 72, 1190, 1224, 1260, 1888, 1912
ApoI	RAATTY	3 cut(s)	55, 265, 1771
AsiGI	ACCGGT	1 cut(s)	332
Asp700I	GAANNNNTTC	1 cut(s)	1284
Asp718I	GGTACC	2 cut(s)	16, 872
AspLEI	GCGC	5 cut(s)	227, 421, 622, 1888, 1927
AspS9I	GGNCC	4 cut(s)	211, 341, 780, 1274
AsuC2I	CCSGG	1 cut(s)	805
AsuHPI	GGTGA	3 cut(s)	322, 659, 664
AvaI	CYCGRG	1 cut(s)	1270
AvaII	GGWCC	3 cut(s)	211, 780, 1274
BalI	TGGCCA	2 cut(s)	1517, 1605
BanI	GGYRCC	2 cut(s)	16, 872
BanII	GRGCYC	1 cut(s)	725
BauI	CACGAG	1 cut(s)	556
BbsI	GAAGAC	1 cut(s)	212
Bbv12I	GWGCWC	2 cut(s)	243, 725
BbvI	GCAGC	7 cut(s)	59, 81, 1202, 1236, 1247, 1875, 1899
BccI	CCATC	6 cut(s)	56, 661, 944, 1133, 1700, 2003
BceAI	ACGGC	2 cut(s)	373, 1993
BciT130I	CCWGG	2 cut(s)	1519, 1726
BciVI	GTATCC	1 cut(s)	1568
BcnI	CCSGG	1 cut(s)	805
BcoDI	GTCTC	2 cut(s)	372, 1443
BfaI	CTAG	4 cut(s)	176, 362, 392, 1595
BfmI	CTRYAG	6 cut(s)	499, 1261, 1630, 1692, 1749, 1889
BfoI	RGCGCY	1 cut(s)	1889
BfuAI	ACCTGC	2 cut(s)	583, 1930
BfuI	GTATCC	1 cut(s)	1568
BisI	GCNGC	7 cut(s)	70, 73, 1191, 1225, 1261, 1889, 1913
BlsI	GCNGC	7 cut(s)	71, 74, 1192, 1226, 1262, 1890, 1914
BmcAI	AGTACT	2 cut(s)	1046, 1700
Bme1390I	CCNGG	3 cut(s)	805, 1519, 1726
Bme18I	GGWCC	3 cut(s)	211, 780, 1274
BmeT110I	CYCGRG	1 cut(s)	1270
BmgT120I	GGNCC	4 cut(s)	211, 341, 780, 1274
BmiI	GGNNCC	6 cut(s)	18, 343, 580, 874, 1275, 1584
BmrFI	CCNGG	3 cut(s)	805, 1519, 1726
BmrI	ACTGGG	2 cut(s)	462, 1372
BmsI	GCATC	5 cut(s)	758, 758, 764, 985, 1884
BmuI	ACTGGG	2 cut(s)	462, 1372
BpiI	GAAGAC	1 cut(s)	212
BplI	GAGNNNNNCTC	4 cut(s)	822, 854, 1844, 1876
BpmI	CTGGAG	3 cut(s)	724, 850, 1569
BpuMI	CCSGG	1 cut(s)	805
BsaI	GGTCTC	1 cut(s)	372
BsaWI	WCCGGW	1 cut(s)	332
BsaXI	ACNNNNNCTCC	2 cut(s)	312, 342
Bsc4I	CCNNNNNNNGG	2 cut(s)	452, 588
Bse118I	RCCGGY	1 cut(s)	332
Bse1I	ACTGG	4 cut(s)	457, 1367, 1606, 1669
BseBI	CCWGG	2 cut(s)	1519, 1726
BseGI	GGATG	6 cut(s)	67, 98, 672, 721, 773, 1813
BseLI	CCNNNNNNNGG	2 cut(s)	452, 588
BseMII	CTCAG	1 cut(s)	1716
BseNI	ACTGG	4 cut(s)	457, 1367, 1606, 1669
BseRI	GAGGAG	2 cut(s)	527, 1384
BseXI	GCAGC	7 cut(s)	59, 81, 1202, 1236, 1247, 1875, 1899
BsgI	GTGCAG	1 cut(s)	1898
Bsh1236I	CGCG	1 cut(s)	421
BshFI	GGCC	5 cut(s)	342, 360, 1064, 1517, 1605
BshNI	GGYRCC	2 cut(s)	16, 872
BshTI	ACCGGT	1 cut(s)	332
BsiHKAI	GWGCWC	2 cut(s)	243, 725
BsiHKCI	CYCGRG	1 cut(s)	1270
BsiSI	CCGG	3 cut(s)	312, 333, 804
BslFI	GGGAC	2 cut(s)	1287, 1759
BslI	CCNNNNNNNGG	2 cut(s)	452, 588
BsmAI	GTCTC	2 cut(s)	372, 1443
BsmFI	GGGAC	2 cut(s)	1287, 1759
BsnI	GGCC	5 cut(s)	342, 360, 1064, 1517, 1605
Bso31I	GGTCTC	1 cut(s)	372
BsoBI	CYCGRG	1 cut(s)	1270
Bsp1286I	GDGCHC	2 cut(s)	243, 725
Bsp1407I	TGTACA	1 cut(s)	798
Bsp143I	GATC	7 cut(s)	162, 193, 524, 986, 1336, 1437, 1656
Bsp19I	CCATGG	1 cut(s)	1959
BspACI	CCGC	3 cut(s)	155, 608, 1324
BspANI	GGCC	5 cut(s)	342, 360, 1064, 1517, 1605
BspCNI	CTCAG	1 cut(s)	1715
BspFNI	CGCG	1 cut(s)	421
BspHI	TCATGA	1 cut(s)	483
BspLI	GGNNCC	6 cut(s)	18, 343, 580, 874, 1275, 1584
BspMAI	CTGCAG	3 cut(s)	1265, 1696, 1893
BspMI	ACCTGC	2 cut(s)	583, 1930
BspPI	GGATC	5 cut(s)	157, 188, 532, 994, 1445
BspT107I	GGYRCC	2 cut(s)	16, 872
BspTNI	GGTCTC	1 cut(s)	372
BsrFI	RCCGGY	1 cut(s)	332
BsrGI	TGTACA	1 cut(s)	798
BsrI	ACTGG	4 cut(s)	457, 1367, 1606, 1669
BssAI	RCCGGY	1 cut(s)	332
BssMI	GATC	7 cut(s)	162, 193, 524, 986, 1336, 1437, 1656
BssSI	CACGAG	1 cut(s)	556
BssT1I	CCWWGG	4 cut(s)	776, 909, 1959, 2054
Bst2BI	CACGAG	1 cut(s)	556
Bst2UI	CCWGG	2 cut(s)	1519, 1726
Bst6I	CTCTTC	4 cut(s)	393, 557, 976, 1154
BstAPI	GCANNNNNTGC	1 cut(s)	2012
BstAUI	TGTACA	1 cut(s)	798
BstC8I	GCNNGC	5 cut(s)	606, 773, 792, 1462, 2062
BstDEI	CTNAG	1 cut(s)	1702
BstDSI	CCRYGG	3 cut(s)	1065, 1959, 1975
BstENI	CCTNNNNNAGG	1 cut(s)	586
BstF5I	GGATG	6 cut(s)	67, 98, 672, 721, 773, 1813
BstFNI	CGCG	1 cut(s)	421
BstH2I	RGCGCY	1 cut(s)	1889
BstHHI	GCGC	5 cut(s)	227, 421, 622, 1888, 1927
BstKTI	GATC	7 cut(s)	165, 196, 527, 989, 1339, 1440, 1659
BstMAI	GTCTC	2 cut(s)	372, 1443
BstMBI	GATC	7 cut(s)	162, 193, 524, 986, 1336, 1437, 1656
BstMWI	GCNNNNNNNGC	6 cut(s)	427, 578, 1266, 1499, 1894, 2012
BstNI	CCWGG	2 cut(s)	1519, 1726
BstSCI	CCNGG	3 cut(s)	803, 1517, 1724
BstSFI	CTRYAG	6 cut(s)	499, 1261, 1630, 1692, 1749, 1889
BstUI	CGCG	1 cut(s)	421
BstV1I	GCAGC	7 cut(s)	59, 81, 1202, 1236, 1247, 1875, 1899
BstV2I	GAAGAC	1 cut(s)	212
BstX2I	RGATCY	2 cut(s)	162, 193
BstYI	RGATCY	2 cut(s)	162, 193
BsuI	GTATCC	1 cut(s)	1568
BsuRI	GGCC	5 cut(s)	342, 360, 1064, 1517, 1605
BtgI	CCRYGG	3 cut(s)	1065, 1959, 1975
BtsCI	GGATG	6 cut(s)	67, 98, 672, 721, 773, 1813
BtsI	GCAGTG	1 cut(s)	1161
BtsIMutI	CAGTG	6 cut(s)	298, 432, 450, 740, 1161, 1662
BveI	ACCTGC	2 cut(s)	583, 1930
Cac8I	GCNNGC	5 cut(s)	606, 773, 792, 1462, 2062
CaiI	CAGNNNCTG	1 cut(s)	1748
CciI	TCATGA	1 cut(s)	483
CfoI	GCGC	5 cut(s)	227, 421, 622, 1888, 1927
Cfr10I	RCCGGY	1 cut(s)	332
Cfr13I	GGNCC	4 cut(s)	211, 341, 780, 1274
Csp6I	GTAC	7 cut(s)	17, 423, 799, 873, 1045, 1699, 1762
CspAI	ACCGGT	1 cut(s)	332
CviAII	CATG	5 cut(s)	484, 1579, 1601, 1793, 1960
CviQI	GTAC	7 cut(s)	17, 423, 799, 873, 1045, 1699, 1762
DdeI	CTNAG	1 cut(s)	1702
DpnI	GATC	7 cut(s)	164, 195, 526, 988, 1338, 1439, 1658
DpnII	GATC	7 cut(s)	162, 193, 524, 986, 1336, 1437, 1656
DraIII	CACNNNGTG	1 cut(s)	807
EaeI	YGGCCR	2 cut(s)	1515, 1603
Eam1104I	CTCTTC	4 cut(s)	393, 557, 976, 1154
EarI	CTCTTC	4 cut(s)	393, 557, 976, 1154
Ecl136II	GAGCTC	1 cut(s)	723
Eco130I	CCWWGG	4 cut(s)	776, 909, 1959, 2054
Eco24I	GRGCYC	1 cut(s)	725
Eco31I	GGTCTC	1 cut(s)	372
Eco32I	GATATC	1 cut(s)	1381
Eco47I	GGWCC	3 cut(s)	211, 780, 1274
Eco53kI	GAGCTC	1 cut(s)	723
Eco57I	CTGAAG	2 cut(s)	179, 2004
Eco88I	CYCGRG	1 cut(s)	1270
EcoICRI	GAGCTC	1 cut(s)	723
EcoNI	CCTNNNNNAGG	1 cut(s)	586
EcoO109I	RGGNCCY	2 cut(s)	341, 1274
EcoRII	CCWGG	2 cut(s)	1517, 1724
EcoRV	GATATC	1 cut(s)	1381
EcoT14I	CCWWGG	4 cut(s)	776, 909, 1959, 2054
EcoT22I	ATGCAT	2 cut(s)	6, 751
EcoT38I	GRGCYC	1 cut(s)	725
ErhI	CCWWGG	4 cut(s)	776, 909, 1959, 2054
FaeI	CATG	5 cut(s)	487, 1582, 1604, 1796, 1963
FaqI	GGGAC	2 cut(s)	1287, 1759
FatI	CATG	5 cut(s)	483, 1578, 1600, 1792, 1959
FauNDI	CATATG	1 cut(s)	991
Fnu4HI	GCNGC	7 cut(s)	70, 73, 1191, 1225, 1261, 1889, 1913
FokI	GGATG	6 cut(s)	74, 85, 679, 728, 780, 1820
FriOI	GRGCYC	1 cut(s)	725
Fsp4HI	GCNGC	7 cut(s)	70, 73, 1191, 1225, 1261, 1889, 1913
FspBI	CTAG	4 cut(s)	176, 362, 392, 1595
GlaI	GCGC	5 cut(s)	226, 420, 621, 1887, 1926
GluI	GCNGC	7 cut(s)	70, 73, 1191, 1225, 1261, 1889, 1913
GsuI	CTGGAG	3 cut(s)	724, 850, 1569
HaeII	RGCGCY	1 cut(s)	1889
HaeIII	GGCC	5 cut(s)	342, 360, 1064, 1517, 1605
HapII	CCGG	3 cut(s)	312, 333, 804
HhaI	GCGC	5 cut(s)	227, 421, 622, 1888, 1927
Hin1II	CATG	5 cut(s)	487, 1582, 1604, 1796, 1963
Hin6I	GCGC	5 cut(s)	225, 419, 620, 1886, 1925
HinP1I	GCGC	5 cut(s)	225, 419, 620, 1886, 1925
HincII	GTYRAC	2 cut(s)	1087, 1611
HindII	GTYRAC	2 cut(s)	1087, 1611
HindIII	AAGCTT	1 cut(s)	1091
HinfI	GANTC	5 cut(s)	614, 677, 1315, 1478, 1540
HpaII	CCGG	3 cut(s)	312, 333, 804
HphI	GGTGA	3 cut(s)	322, 659, 664
Hpy166II	GTNNAC	4 cut(s)	296, 801, 1087, 1611
Hpy188III	TCNNGA	9 cut(s)	392, 484, 867, 952, 1272, 1319, 1340, 1418, 1586
Hpy8I	GTNNAC	4 cut(s)	296, 801, 1087, 1611
Hpy99I	CGWCG	2 cut(s)	476, 1985
HpyAV	CCTTC	7 cut(s)	7, 56, 1295, 1394, 1424, 1662, 1900
HpyCH4IV	ACGT	2 cut(s)	126, 235
HpyF10VI	GCNNNNNNNGC	6 cut(s)	427, 578, 1266, 1499, 1894, 2012
HpyF3I	CTNAG	1 cut(s)	1702
HpySE526I	ACGT	2 cut(s)	126, 235
Hsp92II	CATG	5 cut(s)	487, 1582, 1604, 1796, 1963
HspAI	GCGC	5 cut(s)	225, 419, 620, 1886, 1925
KpnI	GGTACC	2 cut(s)	20, 876
Kzo9I	GATC	7 cut(s)	162, 193, 524, 986, 1336, 1437, 1656
LmnI	GCTCC	6 cut(s)	578, 869, 911, 1588, 1683, 1909
Lsp1109I	GCAGC	7 cut(s)	59, 81, 1202, 1236, 1247, 1875, 1899
LweI	GCATC	5 cut(s)	758, 758, 764, 985, 1884
MaeI	CTAG	4 cut(s)	176, 362, 392, 1595
MaeII	ACGT	2 cut(s)	126, 235
MaeIII	GTNAC	5 cut(s)	171, 328, 1541, 1714, 1991
MalI	GATC	7 cut(s)	164, 195, 526, 988, 1338, 1439, 1658
MboI	GATC	7 cut(s)	162, 193, 524, 986, 1336, 1437, 1656
MfeI	CAATTG	2 cut(s)	1053, 1557
MflI	RGATCY	2 cut(s)	162, 193
MhlI	GDGCHC	2 cut(s)	243, 725
MlsI	TGGCCA	2 cut(s)	1517, 1605
MluNI	TGGCCA	2 cut(s)	1517, 1605
MlyI	GAGTC	4 cut(s)	623, 686, 1324, 1549
MmeI	TCCRAC	4 cut(s)	169, 440, 907, 1369
Mox20I	TGGCCA	2 cut(s)	1517, 1605
Mph1103I	ATGCAT	2 cut(s)	6, 751
MroXI	GAANNNNTTC	1 cut(s)	1284
MscI	TGGCCA	2 cut(s)	1517, 1605
MseI	TTAA	5 cut(s)	80, 1034, 1359, 1775, 1803
MslI	CAYNNNNRTG	2 cut(s)	251, 1797
Msp20I	TGGCCA	2 cut(s)	1517, 1605
MspA1I	CMGCKG	2 cut(s)	72, 157
MspI	CCGG	3 cut(s)	312, 333, 804
MspR9I	CCNGG	3 cut(s)	805, 1519, 1726
MunI	CAATTG	2 cut(s)	1053, 1557
MvaI	CCWGG	2 cut(s)	1519, 1726
MvnI	CGCG	1 cut(s)	421
MwoI	GCNNNNNNNGC	6 cut(s)	427, 578, 1266, 1499, 1894, 2012
NciI	CCSGG	1 cut(s)	805
NcoI	CCATGG	1 cut(s)	1959
NdeI	CATATG	1 cut(s)	991
NdeII	GATC	7 cut(s)	162, 193, 524, 986, 1336, 1437, 1656
NlaIII	CATG	5 cut(s)	487, 1582, 1604, 1796, 1963
NlaIV	GGNNCC	6 cut(s)	18, 343, 580, 874, 1275, 1584
NmeAIII	GCCGAG	2 cut(s)	439, 563
NmuCI	GTSAC	2 cut(s)	328, 1541
NsiI	ATGCAT	2 cut(s)	6, 751
OliI	CACNNNNGTG	1 cut(s)	251
PagI	TCATGA	1 cut(s)	483
PdmI	GAANNNNTTC	1 cut(s)	1284
PfeI	GAWTC	1 cut(s)	1478
PflFI	GACNNNGTC	1 cut(s)	1082
PinAI	ACCGGT	1 cut(s)	332
PkrI	GCNGC	7 cut(s)	71, 74, 1192, 1226, 1262, 1890, 1914
PleI	GAGTC	4 cut(s)	622, 685, 1323, 1548
PpsI	GAGTC	4 cut(s)	622, 685, 1323, 1548
PpuMI	RGGWCCY	1 cut(s)	1274
PsiI	TTATAA	2 cut(s)	1122, 1220
Psp124BI	GAGCTC	1 cut(s)	725
Psp1406I	AACGTT	1 cut(s)	126
Psp5II	RGGWCCY	1 cut(s)	1274
Psp6I	CCWGG	2 cut(s)	1517, 1724
PspGI	CCWGG	2 cut(s)	1517, 1724
PspN4I	GGNNCC	6 cut(s)	18, 343, 580, 874, 1275, 1584
PspPI	GGNCC	4 cut(s)	211, 341, 780, 1274
PspPPI	RGGWCCY	1 cut(s)	1274
PstI	CTGCAG	3 cut(s)	1265, 1696, 1893
PstNI	CAGNNNCTG	1 cut(s)	1748
PsuI	RGATCY	2 cut(s)	162, 193
PsyI	GACNNNGTC	1 cut(s)	1082
PvuII	CAGCTG	1 cut(s)	72
RsaI	GTAC	7 cut(s)	18, 424, 800, 874, 1046, 1700, 1763
RsaNI	GTAC	7 cut(s)	17, 423, 799, 873, 1045, 1699, 1762
RseI	CAYNNNNRTG	2 cut(s)	251, 1797
SacI	GAGCTC	1 cut(s)	725
SaqAI	TTAA	5 cut(s)	80, 1034, 1359, 1775, 1803
SatI	GCNGC	7 cut(s)	70, 73, 1191, 1225, 1261, 1889, 1913
Sau3AI	GATC	7 cut(s)	162, 193, 524, 986, 1336, 1437, 1656
Sau96I	GGNCC	4 cut(s)	211, 341, 780, 1274
ScaI	AGTACT	2 cut(s)	1046, 1700
SchI	GAGTC	4 cut(s)	623, 686, 1324, 1549
ScrFI	CCNGG	3 cut(s)	805, 1519, 1726
SduI	GDGCHC	2 cut(s)	243, 725
SfaNI	GCATC	5 cut(s)	758, 758, 764, 985, 1884
SfcI	CTRYAG	6 cut(s)	499, 1261, 1630, 1692, 1749, 1889
SinI	GGWCC	3 cut(s)	211, 780, 1274
SmiMI	CAYNNNNRTG	2 cut(s)	251, 1797
SsiI	CCGC	3 cut(s)	155, 608, 1324
SspMI	CTAG	4 cut(s)	176, 362, 392, 1595
SstI	GAGCTC	1 cut(s)	725
StyD4I	CCNGG	3 cut(s)	803, 1517, 1724
StyI	CCWWGG	4 cut(s)	776, 909, 1959, 2054
TaiI	ACGT	2 cut(s)	129, 238
TaqI	TCGA	3 cut(s)	471, 1335, 1527
TatI	WGTACW	3 cut(s)	798, 1044, 1698
TfiI	GAWTC	1 cut(s)	1478
Tru1I	TTAA	5 cut(s)	80, 1034, 1359, 1775, 1803
Tru9I	TTAA	5 cut(s)	80, 1034, 1359, 1775, 1803
TscAI	CASTG	6 cut(s)	298, 432, 457, 740, 1161, 1669
TseFI	GTSAC	2 cut(s)	328, 1541
TseI	GCWGC	7 cut(s)	69, 72, 1190, 1224, 1260, 1888, 1912
Tsp45I	GTSAC	2 cut(s)	328, 1541
TspDTI	ATGAA	8 cut(s)	38, 87, 128, 472, 1221, 1311, 1318, 1965
TspRI	CASTG	6 cut(s)	298, 432, 457, 740, 1161, 1669
Tth111I	GACNNNGTC	1 cut(s)	1082
VpaK11BI	GGWCC	3 cut(s)	211, 780, 1274
XagI	CCTNNNNNAGG	1 cut(s)	586
XapI	RAATTY	3 cut(s)	55, 265, 1771
XbaI	TCTAGA	1 cut(s)	391
XmnI	GAANNNNTTC	1 cut(s)	1284
XspI	CTAG	4 cut(s)	176, 362, 392, 1595
ZrmI	AGTACT	2 cut(s)	1046, 1700
Zsp2I	ATGCAT	2 cut(s)	6, 751

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.