Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0006595

Physical Location & Seq

Genomic Coordinates Reverse (-)

6793420 .. 6796085

Gene Structure 2666 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1097400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2016 bp

ATGAAGAACTGCAACATTGCAGCTCTTTCACAAAATTTCCTTCTCCTGAAGATCCTGCTGTTCCTAGGAATGGCTGCCGGAGATCCAAGAACGAAGACGGTCCAAATAATGTGCGGCAACCAACGTGAGCACAACACAACCGTTTTTGTTCCGAATTTTGTCAGTACGATGGAAAACATCAGTGAACAAATGCGAACTTCTGGCTTTGGCGTGGCAGTAACCGGTTCGGGCCCTGATACAAACTATGGCATAGCTCAATGCTACGGAGATCTTTCTCTTCTAGACTGTGTGTTATGCTACGCCGAGGCGCGTACAGTGCTTCCCCAATGTTTTCCTTACAATGGGGGTCGGATTTACCTCGACGGTTGCTTCATGAGGTCCGAAAACTACAGCTTTTACGATGAGTTTAGAGGATCGGAGGACAGGGTCGTGTGTGGGAACACGACAAGGAAGAGTTCAGCGTTCGGAGAATCAGCAAGGGAGGTTGTGAGGCGTGCGGTTGAGTCTGCACCAAGCAACCGAGGCTATGCAAGGGGTGAGGTGAGTGTGAGTGGGACAGCGAACCAATCGGCTTACGTGTTGGCAGATTGCTGGAGAACTTTGGATGAGAGCTCTTGCAGACAGTGTTTAGAGAATGCATCTGCGTCAATGTTGGGATGCTTGCCTTGGTCCGAGGGGCGAGCATTGTACACCGGGTGCTTCATGAGGTATTCGGACACAGATTTTCTCAACAAGGAAATGGGAAATGGGAGTTCCAGAGATATATACAAAAGAAAAGAAGAGGTAAAAAGCTCGTTGAAACTCTGCAATGCAGCCAGATTGTACTTAGTTATTGCATTGCTTCATCAATGGTGCAAATTTAATAACGTCTACAATGGACGAAATGGAATTTCACACGTAAGAGATTACCGACCGCCAAAAGCTTTTCGGGGTTTTGCAGGAACAAATGATGCAGAAAAGTGGGCGAAAACCCTAAATGACAGTAGCTTAAACTTCAAGTACTCAACAATTGAAAAGGCCACAGGGTCTTTTGACATTGTCAACAAGCTTGGGCAAGGAGGTTTCGGAACTGTTTACAAAGGAGTTCTGGCTGATGGGAGAGAGATTGCAGTGAAGAGGCTTTTCTTCAACAACAGGCACAGAGCAGCAGATTTCTATAACGAAATCAACATTATAAGCAGCGTCGAACACAAAAACTTGGTCAGGTTGCTGGGTTGTTGTTGTGCGGGACCTGAAAGCCTTCTCGTCTATGAATACCTACCAAACAGAAGTCTAGATCGCTTCATCTTCGATCAAGAAAGGGCTAAAACACTTAACTGGGAGAGGAGATATGAGATCATTGTTGGGACAGCAGAAGGTTTAGTCTACCTTCATGACAACTCGAAAACAAGGATTATTCACAGAGACATAAAGGCCAGCAACATCCTTTTGGATTCAAGGCTTCGTGCTAAAATTGCAGATTTTGGGTTGGCCAGGTCTTTCGAGGAAGATAAGAGTCACATCAGCACAGCCATTGCAGGAACATTGGGATATATGGCTCCTGAATACCTAGCTCATGGCCAGTTGACAGAAAAGGCAGATGTCTACAGCTTCGGAGTGCTTATATTGGAGATCATCACTGGAAGGCAGAACAACAGGAGCACATCTGCAGAGTACTCAGACGGCATAGTTATAATTACCTGGAAGCACTTTCAAGCAGGGACTGTAGAGGAACTATACGACCCAAACTTAATGTCGCAAAATTTCCATGACAACTTTAAGGATGAGATATTCAGAGTGGTCCAGATAGGACTTCTATGCACCCAAGAGAGTCCCTCTTTACGACCAACAATGTCAAAGGCGCTGCAGATGCTAACAAAGAAGGAGCTGGACCTTCCTCCACCTGCCAATCCACCTTTTATAGATGAAAAGACCATGGAACTCAATGACAACGGCGACGACCAATGTTACCCTCTAAATGCAGATGGTGCTTCTTCAGTTGCGAGTGTTTCACATAGTTCTTTCTATCCTAGGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000302 GO:0001101 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005623 GO:0005886 GO:0005911 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006979 GO:0008150 GO:0008152 GO:0009506 GO:0009987 GO:0010035 GO:0010193 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0030054 GO:0036211 GO:0042221 GO:0042493 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0046677 GO:0046777 GO:0050896 GO:0055044 GO:0071704 GO:0071944 GO:0140096 GO:1901564 GO:1901700

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Pkinase Pkinase_Tyr Stress-antifung

Protein Analysis

672

Amino Acids

75.12

Weight (kDa)

6.86

Isoelectric Point (pI)

35.72

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Stress-antifung	PF01657	38 - 130	2.7e-12	Salt stress response/antifungal
Stress-antifung	PF01657	145 - 239	2.1e-11	Salt stress response/antifungal
Pkinase	PF00069	345 - 616	4.9e-48	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	346 - 616	1.2e-48	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006595)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G70520 AT4G28670
fragaria_vesca	FvH4_4g26071 FvH4_4g26071 FvH4_4g26071
malus_domestica	MD13G1096000.v1.1 MD16G1097400.v1.1
prunus_persica	Prupe.1G249400_v2.0.a1 Prupe.1G249400_v2.0.a1 Prupe.1G249400_v2.0.a1
pyrus_communis	pycom13g08370 pycom13g08380 pycom13g08410
rosa_chinensis	RchiOBHm_Chr4g0433531
rosa_laevigata	RLG00000006745
rosa_multiflora	Rmu_sc0000312.1_g000009
rosa_roxburghii	Rroxscaffold_5G00375050
rosa_rugosa	Rorug04G0269400 Rorug04G0269500 Rorug04G0269600
rosa_samantha	Rh4AG324200 Rh4BG332200 Rh4CG347500 Rh4DG328500
rosa_wichuraiana	Rw0G020170 Rw4G028080

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	1175, 1673
AarI	CACCTGC	1 cut(s)	1891
Acc36I	ACCTGC	1 cut(s)	1891
AccI	GTMKAC	3 cut(s)	870, 1365, 1584
AccII	CGCG	1 cut(s)	310
AciI	CCGC	4 cut(s)	114, 497, 914, 1226
AclWI	GGATC	3 cut(s)	46, 77, 421
AcoI	YGGCCR	2 cut(s)	1470, 1558
AcsI	RAATTY	5 cut(s)	34, 154, 857, 888, 1741
AcuI	CTGAAG	2 cut(s)	68, 1959
AdeI	CACNNNGTG	1 cut(s)	696
AfaI	GTAC	6 cut(s)	166, 313, 689, 824, 1001, 1655
AfiI	CCNNNNNNNGG	2 cut(s)	70, 341
AflIII	ACRYGT	2 cut(s)	576, 895
AgeI	ACCGGT	1 cut(s)	221
AgsI	TTSAA	6 cut(s)	799, 997, 1013, 1129, 1437, 1694
AjnI	CCWGG	2 cut(s)	1472, 1679
Alw21I	GWGCWC	3 cut(s)	132, 614, 1643
Alw26I	GTCTC	1 cut(s)	1398
AlwI	GGATC	3 cut(s)	46, 77, 421
AlwNI	CAGNNNCTG	1 cut(s)	1703
AoxI	GGCC	5 cut(s)	229, 1017, 1413, 1470, 1558
ApaI	GGGCCC	1 cut(s)	233
ApeKI	GCWGC	6 cut(s)	20, 74, 812, 1145, 1179, 1843
ApoI	RAATTY	5 cut(s)	34, 154, 857, 888, 1741
ArsI	GACNNNNNNTTYG	4 cut(s)	1185, 1217, 1718, 1750
AsiGI	ACCGGT	1 cut(s)	221
Asp700I	GAANNNNTTC	1 cut(s)	1239
AspA2I	CCTAGG	2 cut(s)	64, 2009
AspLEI	GCGC	2 cut(s)	310, 1843
AspS9I	GGNCC	8 cut(s)	100, 229, 230, 378, 669, 1229, 1780, 1870
AsuC2I	CCSGG	1 cut(s)	694
AsuHPI	GGTGA	2 cut(s)	548, 553
AvaII	GGWCC	6 cut(s)	100, 378, 669, 1229, 1780, 1870
AvrII	CCTAGG	2 cut(s)	64, 2009
BaeGI	GKGCMC	1 cut(s)	233
BalI	TGGCCA	2 cut(s)	1472, 1560
BanII	GRGCYC	2 cut(s)	233, 614
BbsI	GAAGAC	1 cut(s)	101
Bbv12I	GWGCWC	3 cut(s)	132, 614, 1643
BbvI	GCAGC	6 cut(s)	32, 61, 824, 1157, 1191, 1830
BccI	CCATC	3 cut(s)	163, 1088, 1958
BceAI	ACGGC	2 cut(s)	1678, 1948
BciT130I	CCWGG	2 cut(s)	1474, 1681
BcnI	CCSGG	1 cut(s)	694
BcoDI	GTCTC	1 cut(s)	1398
BfaI	CTAG	5 cut(s)	65, 281, 1274, 1550, 2010
BfmI	CTRYAG	5 cut(s)	388, 1585, 1647, 1704, 1844
BfoI	RGCGCY	1 cut(s)	1844
BfuAI	ACCTGC	1 cut(s)	1891
BglII	AGATCT	1 cut(s)	268
BisI	GCNGC	7 cut(s)	21, 75, 115, 813, 1146, 1180, 1844
BlnI	CCTAGG	2 cut(s)	64, 2009
BlsI	GCNGC	7 cut(s)	22, 76, 116, 814, 1147, 1181, 1845
BmcAI	AGTACT	2 cut(s)	1001, 1655
Bme1390I	CCNGG	3 cut(s)	694, 1474, 1681
Bme18I	GGWCC	6 cut(s)	100, 378, 669, 1229, 1780, 1870
BmgT120I	GGNCC	8 cut(s)	100, 229, 230, 378, 669, 1229, 1780, 1870
BmiI	GGNNCC	3 cut(s)	231, 1230, 1539
BmrFI	CCNGG	3 cut(s)	694, 1474, 1681
BmrI	ACTGGG	1 cut(s)	1327
BmsI	GCATC	4 cut(s)	647, 647, 940, 1839
BmuI	ACTGGG	1 cut(s)	1327
BpiI	GAAGAC	1 cut(s)	101
BplI	GAGNNNNNCTC	2 cut(s)	1799, 1831
BpmI	CTGGAG	1 cut(s)	613
BpuMI	CCSGG	1 cut(s)	694
BsaAI	YACGTR	2 cut(s)	577, 898
BsaJI	CCNNGG	7 cut(s)	64, 303, 520, 665, 672, 1914, 2009
BsaWI	WCCGGW	1 cut(s)	221
Bsc4I	CCNNNNNNNGG	2 cut(s)	70, 341
Bse118I	RCCGGY	1 cut(s)	221
Bse1I	ACTGG	3 cut(s)	1322, 1561, 1624
Bse3DI	GCAATG	4 cut(s)	15, 814, 836, 1512
BseBI	CCWGG	2 cut(s)	1474, 1681
BseDI	CCNNGG	7 cut(s)	64, 303, 520, 665, 672, 1914, 2009
BseGI	GGATG	4 cut(s)	610, 662, 1422, 1768
BseLI	CCNNNNNNNGG	2 cut(s)	70, 341
BseMI	GCAATG	4 cut(s)	15, 814, 836, 1512
BseMII	CTCAG	1 cut(s)	1671
BseNI	ACTGG	3 cut(s)	1322, 1561, 1624
BseRI	GAGGAG	1 cut(s)	1339
BseSI	GKGCMC	1 cut(s)	233
BseXI	GCAGC	6 cut(s)	32, 61, 824, 1157, 1191, 1830
BseYI	CCCAGC	1 cut(s)	1210
BsgI	GTGCAG	1 cut(s)	492
Bsh1236I	CGCG	1 cut(s)	310
Bsh1285I	CGRYCG	1 cut(s)	914
BshFI	GGCC	5 cut(s)	231, 1019, 1415, 1472, 1560
BshTI	ACCGGT	1 cut(s)	221
BsiEI	CGRYCG	1 cut(s)	914
BsiHKAI	GWGCWC	3 cut(s)	132, 614, 1643
BsiSI	CCGG	3 cut(s)	78, 222, 693
BslFI	GGGAC	5 cut(s)	568, 1242, 1360, 1714, 1797
BslI	CCNNNNNNNGG	2 cut(s)	70, 341
BsmAI	GTCTC	1 cut(s)	1398
BsmFI	GGGAC	5 cut(s)	568, 1242, 1360, 1714, 1797
BsmI	GAATGC	1 cut(s)	640
BsnI	GGCC	5 cut(s)	231, 1019, 1415, 1472, 1560
Bsp120I	GGGCCC	1 cut(s)	229
Bsp1286I	GDGCHC	4 cut(s)	132, 233, 614, 1643
Bsp1407I	TGTACA	1 cut(s)	687
Bsp143I	GATC	8 cut(s)	51, 82, 268, 413, 1276, 1291, 1335, 1611
Bsp19I	CCATGG	1 cut(s)	1914
BspACI	CCGC	4 cut(s)	114, 497, 914, 1226
BspANI	GGCC	5 cut(s)	231, 1019, 1415, 1472, 1560
BspCNI	CTCAG	1 cut(s)	1670
BspFNI	CGCG	1 cut(s)	310
BspHI	TCATGA	3 cut(s)	372, 702, 1372
BspLI	GGNNCC	3 cut(s)	231, 1230, 1539
BspMAI	CTGCAG	2 cut(s)	1651, 1848
BspMI	ACCTGC	1 cut(s)	1891
BspPI	GGATC	3 cut(s)	46, 77, 421
BsrDI	GCAATG	4 cut(s)	15, 814, 836, 1512
BsrFI	RCCGGY	1 cut(s)	221
BsrGI	TGTACA	1 cut(s)	687
BsrI	ACTGG	3 cut(s)	1322, 1561, 1624
BssAI	RCCGGY	1 cut(s)	221
BssECI	CCNNGG	7 cut(s)	64, 303, 520, 665, 672, 1914, 2009
BssMI	GATC	8 cut(s)	51, 82, 268, 413, 1276, 1291, 1335, 1611
BssT1I	CCWWGG	4 cut(s)	64, 665, 1914, 2009
Bst2UI	CCWGG	2 cut(s)	1474, 1681
Bst4CI	ACNGT	9 cut(s)	100, 142, 287, 316, 365, 624, 983, 1072, 1705
Bst6I	CTCTTC	4 cut(s)	282, 446, 774, 1109
BstAPI	GCANNNNNTGC	1 cut(s)	1967
BstAUI	TGTACA	1 cut(s)	687
BstBAI	YACGTR	2 cut(s)	577, 898
BstC8I	GCNNGC	4 cut(s)	495, 662, 681, 1417
BstDEI	CTNAG	2 cut(s)	826, 1657
BstDSI	CCRYGG	1 cut(s)	1914
BstF5I	GGATG	4 cut(s)	610, 662, 1422, 1768
BstFNI	CGCG	1 cut(s)	310
BstH2I	RGCGCY	1 cut(s)	1844
BstHHI	GCGC	2 cut(s)	310, 1843
BstKTI	GATC	8 cut(s)	54, 85, 271, 416, 1279, 1294, 1338, 1614
BstMAI	GTCTC	1 cut(s)	1398
BstMBI	GATC	8 cut(s)	51, 82, 268, 413, 1276, 1291, 1335, 1611
BstMCI	CGRYCG	1 cut(s)	914
BstMWI	GCNNNNNNNGC	5 cut(s)	316, 522, 1454, 1849, 1967
BstNI	CCWGG	2 cut(s)	1474, 1681
BstSCI	CCNGG	3 cut(s)	692, 1472, 1679
BstSFI	CTRYAG	5 cut(s)	388, 1585, 1647, 1704, 1844
BstSLI	GKGCMC	1 cut(s)	233
BstUI	CGCG	1 cut(s)	310
BstV1I	GCAGC	6 cut(s)	32, 61, 824, 1157, 1191, 1830
BstV2I	GAAGAC	1 cut(s)	101
BstX2I	RGATCY	3 cut(s)	51, 82, 268
BstYI	RGATCY	3 cut(s)	51, 82, 268
BsuRI	GGCC	5 cut(s)	231, 1019, 1415, 1472, 1560
BtgI	CCRYGG	1 cut(s)	1914
BtsCI	GGATG	4 cut(s)	610, 662, 1422, 1768
BtsI	GCAGTG	1 cut(s)	1116
BtsIMutI	CAGTG	5 cut(s)	187, 321, 629, 1116, 1617
BveI	ACCTGC	1 cut(s)	1891
Cac8I	GCNNGC	4 cut(s)	495, 662, 681, 1417
CaiI	CAGNNNCTG	1 cut(s)	1703
CciI	TCATGA	3 cut(s)	372, 702, 1372
CfoI	GCGC	2 cut(s)	310, 1843
Cfr10I	RCCGGY	1 cut(s)	221
Cfr13I	GGNCC	8 cut(s)	100, 229, 230, 378, 669, 1229, 1780, 1870
CseI	GACGC	2 cut(s)	633, 1171
Csp6I	GTAC	6 cut(s)	165, 312, 688, 823, 1000, 1654
CspAI	ACCGGT	1 cut(s)	221
CviAII	CATG	6 cut(s)	373, 703, 1373, 1556, 1748, 1915
CviQI	GTAC	6 cut(s)	165, 312, 688, 823, 1000, 1654
DdeI	CTNAG	2 cut(s)	826, 1657
DpnI	GATC	8 cut(s)	53, 84, 270, 415, 1278, 1293, 1337, 1613
DpnII	GATC	8 cut(s)	51, 82, 268, 413, 1276, 1291, 1335, 1611
DraIII	CACNNNGTG	1 cut(s)	696
EaeI	YGGCCR	2 cut(s)	1470, 1558
Eam1104I	CTCTTC	4 cut(s)	282, 446, 774, 1109
EarI	CTCTTC	4 cut(s)	282, 446, 774, 1109
Ecl136II	GAGCTC	1 cut(s)	612
Eco130I	CCWWGG	4 cut(s)	64, 665, 1914, 2009
Eco24I	GRGCYC	2 cut(s)	233, 614
Eco47I	GGWCC	6 cut(s)	100, 378, 669, 1229, 1780, 1870
Eco53kI	GAGCTC	1 cut(s)	612
Eco57I	CTGAAG	2 cut(s)	68, 1959
EcoICRI	GAGCTC	1 cut(s)	612
EcoO109I	RGGNCCY	2 cut(s)	230, 1229
EcoRII	CCWGG	2 cut(s)	1472, 1679
EcoT14I	CCWWGG	4 cut(s)	64, 665, 1914, 2009
EcoT22I	ATGCAT	1 cut(s)	640
EcoT38I	GRGCYC	2 cut(s)	233, 614
ErhI	CCWWGG	4 cut(s)	64, 665, 1914, 2009
FaeI	CATG	6 cut(s)	376, 706, 1376, 1559, 1751, 1918
FaqI	GGGAC	5 cut(s)	568, 1242, 1360, 1714, 1797
FatI	CATG	6 cut(s)	372, 702, 1372, 1555, 1747, 1914
FauI	CCCGC	1 cut(s)	1219
FblI	GTMKAC	3 cut(s)	870, 1365, 1584
Fnu4HI	GCNGC	7 cut(s)	21, 75, 115, 813, 1146, 1180, 1844
FokI	GGATG	4 cut(s)	617, 669, 1409, 1775
FriOI	GRGCYC	2 cut(s)	233, 614
Fsp4HI	GCNGC	7 cut(s)	21, 75, 115, 813, 1146, 1180, 1844
FspBI	CTAG	5 cut(s)	65, 281, 1274, 1550, 2010
GlaI	GCGC	2 cut(s)	309, 1842
GluI	GCNGC	7 cut(s)	21, 75, 115, 813, 1146, 1180, 1844
GsaI	CCCAGC	1 cut(s)	1214
GsuI	CTGGAG	1 cut(s)	613
HaeII	RGCGCY	1 cut(s)	1844
HaeIII	GGCC	5 cut(s)	231, 1019, 1415, 1472, 1560
HapII	CCGG	3 cut(s)	78, 222, 693
HgaI	GACGC	2 cut(s)	633, 1171
HhaI	GCGC	2 cut(s)	310, 1843
Hin1II	CATG	6 cut(s)	376, 706, 1376, 1559, 1751, 1918
Hin6I	GCGC	2 cut(s)	308, 1841
HinP1I	GCGC	2 cut(s)	308, 1841
HincII	GTYRAC	2 cut(s)	1042, 1566
HindII	GTYRAC	2 cut(s)	1042, 1566
HindIII	AAGCTT	2 cut(s)	921, 1046
HinfI	GANTC	5 cut(s)	470, 503, 1433, 1495, 1810
HpaII	CCGG	3 cut(s)	78, 222, 693
HphI	GGTGA	2 cut(s)	548, 553
Hpy166II	GTNNAC	8 cut(s)	185, 690, 871, 1042, 1075, 1366, 1566, 1585
Hpy8I	GTNNAC	8 cut(s)	185, 690, 871, 1042, 1075, 1366, 1566, 1585
Hpy99I	CGWCG	3 cut(s)	365, 1187, 1940
HpyAV	CCTTC	7 cut(s)	50, 1250, 1349, 1379, 1617, 1855, 1883
HpyCH4III	ACNGT	9 cut(s)	100, 142, 287, 316, 365, 624, 983, 1072, 1705
HpyCH4IV	ACGT	4 cut(s)	124, 576, 867, 897
HpyF10VI	GCNNNNNNNGC	5 cut(s)	316, 522, 1454, 1849, 1967
HpyF3I	CTNAG	2 cut(s)	826, 1657
HpySE526I	ACGT	4 cut(s)	124, 576, 867, 897
Hsp92II	CATG	6 cut(s)	376, 706, 1376, 1559, 1751, 1918
HspAI	GCGC	2 cut(s)	308, 1841
Kzo9I	GATC	8 cut(s)	51, 82, 268, 413, 1276, 1291, 1335, 1611
LmnI	GCTCC	3 cut(s)	1543, 1638, 1864
Lsp1109I	GCAGC	6 cut(s)	32, 61, 824, 1157, 1191, 1830
LweI	GCATC	4 cut(s)	647, 647, 940, 1839
MaeI	CTAG	5 cut(s)	65, 281, 1274, 1550, 2010
MaeII	ACGT	4 cut(s)	124, 576, 867, 897
MaeIII	GTNAC	3 cut(s)	217, 1496, 1946
MalI	GATC	8 cut(s)	53, 84, 270, 415, 1278, 1293, 1337, 1613
MboI	GATC	8 cut(s)	51, 82, 268, 413, 1276, 1291, 1335, 1611
MfeI	CAATTG	1 cut(s)	1008
MflI	RGATCY	3 cut(s)	51, 82, 268
MhlI	GDGCHC	4 cut(s)	132, 233, 614, 1643
MlsI	TGGCCA	2 cut(s)	1472, 1560
MluCI	AATT	8 cut(s)	34, 154, 857, 888, 1008, 1452, 1674, 1741
MluNI	TGGCCA	2 cut(s)	1472, 1560
MlyI	GAGTC	3 cut(s)	512, 1504, 1819
MmeI	TCCRAC	1 cut(s)	329
Mox20I	TGGCCA	2 cut(s)	1472, 1560
Mph1103I	ATGCAT	1 cut(s)	640
MroXI	GAANNNNTTC	1 cut(s)	1239
MscI	TGGCCA	2 cut(s)	1472, 1560
MseI	TTAA	5 cut(s)	861, 989, 1314, 1730, 1758
Msp20I	TGGCCA	2 cut(s)	1472, 1560
MspI	CCGG	3 cut(s)	78, 222, 693
MspR9I	CCNGG	3 cut(s)	694, 1474, 1681
MunI	CAATTG	1 cut(s)	1008
Mva1269I	GAATGC	1 cut(s)	640
MvaI	CCWGG	2 cut(s)	1474, 1681
MvnI	CGCG	1 cut(s)	310
MwoI	GCNNNNNNNGC	5 cut(s)	316, 522, 1454, 1849, 1967
NciI	CCSGG	1 cut(s)	694
NcoI	CCATGG	1 cut(s)	1914
NdeII	GATC	8 cut(s)	51, 82, 268, 413, 1276, 1291, 1335, 1611
NlaIII	CATG	6 cut(s)	376, 706, 1376, 1559, 1751, 1918
NlaIV	GGNNCC	3 cut(s)	231, 1230, 1539
NmeAIII	GCCGAG	1 cut(s)	328
NmuCI	GTSAC	1 cut(s)	1496
NsiI	ATGCAT	1 cut(s)	640
PagI	TCATGA	3 cut(s)	372, 702, 1372
PaqCI	CACCTGC	1 cut(s)	1891
PctI	GAATGC	1 cut(s)	640
PdmI	GAANNNNTTC	1 cut(s)	1239
PfeI	GAWTC	2 cut(s)	470, 1433
PflFI	GACNNNGTC	2 cut(s)	425, 1037
PinAI	ACCGGT	1 cut(s)	221
PkrI	GCNGC	7 cut(s)	22, 76, 116, 814, 1147, 1181, 1845
PleI	GAGTC	3 cut(s)	511, 1503, 1818
PpsI	GAGTC	3 cut(s)	511, 1503, 1818
Ppu21I	YACGTR	2 cut(s)	577, 898
PpuMI	RGGWCCY	1 cut(s)	1229
PsiI	TTATAA	2 cut(s)	1175, 1673
Psp124BI	GAGCTC	1 cut(s)	614
Psp5II	RGGWCCY	1 cut(s)	1229
Psp6I	CCWGG	2 cut(s)	1472, 1679
PspFI	CCCAGC	1 cut(s)	1210
PspGI	CCWGG	2 cut(s)	1472, 1679
PspN4I	GGNNCC	3 cut(s)	231, 1230, 1539
PspOMI	GGGCCC	1 cut(s)	229
PspPI	GGNCC	8 cut(s)	100, 229, 230, 378, 669, 1229, 1780, 1870
PspPPI	RGGWCCY	1 cut(s)	1229
PstI	CTGCAG	2 cut(s)	1651, 1848
PstNI	CAGNNNCTG	1 cut(s)	1703
PsuI	RGATCY	3 cut(s)	51, 82, 268
PsyI	GACNNNGTC	2 cut(s)	425, 1037
RsaI	GTAC	6 cut(s)	166, 313, 689, 824, 1001, 1655
RsaNI	GTAC	6 cut(s)	165, 312, 688, 823, 1000, 1654
SacI	GAGCTC	1 cut(s)	614
SaqAI	TTAA	5 cut(s)	861, 989, 1314, 1730, 1758
SatI	GCNGC	7 cut(s)	21, 75, 115, 813, 1146, 1180, 1844
Sau3AI	GATC	8 cut(s)	51, 82, 268, 413, 1276, 1291, 1335, 1611
Sau96I	GGNCC	8 cut(s)	100, 229, 230, 378, 669, 1229, 1780, 1870
ScaI	AGTACT	2 cut(s)	1001, 1655
SchI	GAGTC	3 cut(s)	512, 1504, 1819
ScrFI	CCNGG	3 cut(s)	694, 1474, 1681
SduI	GDGCHC	4 cut(s)	132, 233, 614, 1643
SfaNI	GCATC	4 cut(s)	647, 647, 940, 1839
SfcI	CTRYAG	5 cut(s)	388, 1585, 1647, 1704, 1844
SinI	GGWCC	6 cut(s)	100, 378, 669, 1229, 1780, 1870
Sse9I	AATT	8 cut(s)	34, 154, 857, 888, 1008, 1452, 1674, 1741
SsiI	CCGC	4 cut(s)	114, 497, 914, 1226
SspMI	CTAG	5 cut(s)	65, 281, 1274, 1550, 2010
SstI	GAGCTC	1 cut(s)	614
StyD4I	CCNGG	3 cut(s)	692, 1472, 1679
StyI	CCWWGG	4 cut(s)	64, 665, 1914, 2009
TaaI	ACNGT	9 cut(s)	100, 142, 287, 316, 365, 624, 983, 1072, 1705
TaiI	ACGT	4 cut(s)	127, 579, 870, 900
TaqI	TCGA	5 cut(s)	360, 1185, 1290, 1382, 1482
TasI	AATT	8 cut(s)	34, 154, 857, 888, 1008, 1452, 1674, 1741
TatI	WGTACW	4 cut(s)	687, 822, 999, 1653
TauI	GCSGC	1 cut(s)	117
TfiI	GAWTC	2 cut(s)	470, 1433
Tru1I	TTAA	5 cut(s)	861, 989, 1314, 1730, 1758
Tru9I	TTAA	5 cut(s)	861, 989, 1314, 1730, 1758
TscAI	CASTG	5 cut(s)	187, 321, 629, 1116, 1624
TseFI	GTSAC	1 cut(s)	1496
TseI	GCWGC	6 cut(s)	20, 74, 812, 1145, 1179, 1843
Tsp45I	GTSAC	1 cut(s)	1496
TspDTI	ATGAA	8 cut(s)	17, 361, 691, 833, 1266, 1273, 1361, 1920
TspGWI	ACGGA	1 cut(s)	279
TspRI	CASTG	5 cut(s)	187, 321, 629, 1116, 1624
Tth111I	GACNNNGTC	2 cut(s)	425, 1037
VpaK11BI	GGWCC	6 cut(s)	100, 378, 669, 1229, 1780, 1870
XapI	RAATTY	5 cut(s)	34, 154, 857, 888, 1741
XbaI	TCTAGA	2 cut(s)	280, 1273
XmaJI	CCTAGG	2 cut(s)	64, 2009
XmiI	GTMKAC	3 cut(s)	870, 1365, 1584
XmnI	GAANNNNTTC	1 cut(s)	1239
XspI	CTAG	5 cut(s)	65, 281, 1274, 1550, 2010
ZrmI	AGTACT	2 cut(s)	1001, 1655
Zsp2I	ATGCAT	1 cut(s)	640

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.