Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr13

Orthogroup ID

OG0014248

Physical Location & Seq

Genomic Coordinates Forward (+)

6987139 .. 6989972

Gene Structure 2834 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD13G1098600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1362 bp

ATGGCCAATCCCTGGCGTGGTGCCGGCGCCTGGGCCGCCGAGGCTGAGCGAGCCGAGGCTGAGGAGCTCGCGGAGGCCGCCCAGGTCGAGTCCCAGAGCTTCCCCAGCCTAAAGGAAGCCTCCAGCGCCAAGCCAAAGAAGAAGAAGATGAGCCTCTCCGAATTCTACAACGCTTCCAGCACCCCGGCCTCTAATCGGGTCGGTCTCACTCCCGACGAGATGATGCGCCTCCCTACCGGCCCCAAGGAGCGCTCTGCCGAAGATATGCAGTACGGTCGCCTAGGTGGCGGGTTTTCCTCCTATGGTCGCTCAGGTCCGAATCCGGGTCGGGGCGGGGACGGAGAGGGCGGAGACGGGTCATGGGGTGGTGGTGGAAGAAGATCCTACGGTGGATTCGACGACGACCGGAGGGGCCCGCCTTCTAGGGTTTCTGATTTCGATCAGCCGTCAAGGGCGGACGAGGTTGACAATTGGGCAATGACTAAAAAATCTCTTCCTTCTGAAGATTCGAGTAGGCAGAGCCGGTATAGCTCGCTCGGTAGTGGTGGTGCTGGGGGTGGTGGTGGTGGTATGGGCGCCCCAGCTTGGTCTAGGGCAGACGAGGTTGATAACTGGGCTGTTGGGAAGAAGCCTGCTCCTTCTAGGTCATCCACATTTGGGTCTGGTTTTCGCGATTCGGGTGCAGAGCCGGATCGCTGGGCGAGAGGCGGACGTGAGGTCGACAGAGTTGAGAGAGAGCGACCTAAATTGGTGTTGGATCCTCCGAAAGGCGAGAATGAGCCACTGCAGGTTGTGAAGACCAATAAGCCAAACCCATTCGGAGCAGCTCGACCTAGGGAGGAGATTTTGGCTGAGAAAGGGACGGATTGGAAGAAGCTGGAGTTGGAAATTGAAGCCAAGAAGACGAGCAGGCCGACTAGTGCCCACTCAAGTAGGCCTTCGAGTGCTCAGTCAAGCCGGTCTGAAGGGCCCGGGCTGCATGGGAGTGATAACGCAGTGAAGCCGCGGCCGAAAGTAAACCCCTTTGGTGATGCCAAGCCAAGGGAGGTTTTGCTGGAGGAGCGGGGTAAGGATTGGCGGAAGATTGATCTCGAGCTGGAGCATCAGGGTATTGACAGGCCAGGGTCTGGGGCTGGGAGGCCTGGGTCTGCAGCAGGCAGGGGTGGTAGCTTTCTTGAGAGGACGCCTTCTTATTCCGATTCTAGAAGCATGGAATATGTTGATGGGCCTCCCTCGCAAGGCAAAGGAGATCCGTGGGCAAGGCCTTTTGATGACAGAAGGTCATTCCATGGTGGCAGGGAGAGAAGAGGATTTCTGGGGAACAGAGACATGGGCAGGGCAAGGTCAAGAGAAGGATGGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0003743 GO:0005488 GO:0005515 GO:0005575 GO:0005618 GO:0005623 GO:0005911 GO:0006412 GO:0006413 GO:0006518 GO:0006807 GO:0008135 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009505 GO:0009506 GO:0009987 GO:0010467 GO:0019538 GO:0030054 GO:0030312 GO:0034641 GO:0034645 GO:0042802 GO:0042803 GO:0043043 GO:0043170 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044464 GO:0046983 GO:0055044 GO:0071704 GO:0071944 GO:0097159 GO:1901363 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

eIF-4B

Protein Analysis

454

Amino Acids

48.83

Weight (kDa)

9.34

Isoelectric Point (pI)

52.73

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
eIF-4B	PF06273	1 - 375	1.3e-172	Plant specific eukaryotic initiation factor 4B
eIF-4B	PF06273	379 - 435	1.5e-12	Plant specific eukaryotic initiation factor 4B

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0014248)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G13020
fragaria_vesca	FvH4_4g26000
malus_domestica	MD13G1098600.v1.1 MD16G1099600.v1.1
prunus_persica	Prupe.1G246800_v2.0.a1 Prupe.1G246800_v2.0.a1
pyrus_communis	pycom13g08630 pycom16g08470
rosa_chinensis	RchiOBHm_Chr4g0433421
rosa_laevigata	RLG00000006754
rosa_roxburghii	Rroxscaffold_5G00374970
rosa_rugosa	Rorug04G0268700
rosa_samantha	Rh4AG323400 Rh4BG331300 Rh4CG346600 Rh4DG327600
rosa_wichuraiana	Rw4G028020

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	778
AccB1I	GGYRCC	3 cut(s)	20, 26, 575
AccB7I	CCANNNNNTGG	2 cut(s)	12, 1127
AccBSI	CCGCTC	1 cut(s)	1063
AccI	GTMKAC	1 cut(s)	720
AccII	CGCG	3 cut(s)	71, 672, 1006
AclWI	GGATC	5 cut(s)	375, 699, 752, 765, 1244
AcoI	YGGCCR	2 cut(s)	3, 1007
AcsI	RAATTY	1 cut(s)	161
AcuI	CTGAAG	2 cut(s)	522, 984
AcyI	GRCGYC	3 cut(s)	27, 576, 1184
AfaI	GTAC	1 cut(s)	272
AfeI	AGCGCT	1 cut(s)	251
AgsI	TTSAA	1 cut(s)	893
AhdI	GACNNNNNGTC	2 cut(s)	716, 1279
AhlI	ACTAGT	1 cut(s)	917
AjiI	CACGTC	1 cut(s)	713
AjnI	CCWGG	5 cut(s)	11, 29, 81, 1120, 1141
AjuI	GAANNNNNNNTTGG	2 cut(s)	866, 898
AluBI	AGCT	8 cut(s)	67, 99, 531, 584, 827, 877, 1096, 1170
AluI	AGCT	8 cut(s)	67, 99, 531, 584, 827, 877, 1096, 1170
Alw21I	GWGCWC	2 cut(s)	69, 949
Alw26I	GTCTC	3 cut(s)	209, 345, 1320
AlwI	GGATC	5 cut(s)	375, 699, 752, 765, 1244
AlwNI	CAGNNNCTG	1 cut(s)	1127
Ama87I	CYCGRG	2 cut(s)	971, 1091
Aor51HI	AGCGCT	1 cut(s)	251
ApaI	GGGCCC	2 cut(s)	416, 972
ApeKI	GCWGC	3 cut(s)	824, 976, 1151
ApoI	RAATTY	1 cut(s)	161
AspA2I	CCTAGG	2 cut(s)	280, 833
AspLEI	GCGC	5 cut(s)	29, 128, 228, 252, 578
AspS9I	GGNCC	8 cut(s)	33, 239, 314, 412, 413, 968, 969, 1226
AsuC2I	CCSGG	4 cut(s)	185, 324, 972, 973
AsuHPI	GGTGA	1 cut(s)	1040
AvaI	CYCGRG	2 cut(s)	971, 1091
AvaII	GGWCC	1 cut(s)	314
AvrII	CCTAGG	2 cut(s)	280, 833
BaeGI	GKGCMC	3 cut(s)	416, 925, 972
BalI	TGGCCA	1 cut(s)	5
BamHI	GGATCC	1 cut(s)	757
BanI	GGYRCC	3 cut(s)	20, 26, 575
BanII	GRGCYC	3 cut(s)	69, 416, 972
BbsI	GAAGAC	2 cut(s)	803, 908
Bbv12I	GWGCWC	2 cut(s)	69, 949
BbvCI	CCTCAGC	1 cut(s)	60
BbvI	GCAGC	3 cut(s)	836, 963, 1163
BccI	CCATC	2 cut(s)	1217, 1350
BceAI	ACGGC	1 cut(s)	430
BcgI	CGANNNNNNTGC	4 cut(s)	257, 291, 662, 696
BciT130I	CCWGG	5 cut(s)	13, 31, 83, 1122, 1143
BcnI	CCSGG	4 cut(s)	185, 324, 972, 973
BcoDI	GTCTC	3 cut(s)	209, 345, 1320
BcuI	ACTAGT	1 cut(s)	917
BfaI	CTAG	7 cut(s)	281, 423, 591, 642, 834, 918, 1203
BfmI	CTRYAG	2 cut(s)	785, 1149
BfoI	RGCGCY	4 cut(s)	30, 129, 253, 579
BfuAI	ACCTGC	1 cut(s)	778
BglI	GCCNNNNNGGC	2 cut(s)	41, 285
BisI	GCNGC	7 cut(s)	36, 78, 825, 977, 1004, 1007, 1152
BlnI	CCTAGG	2 cut(s)	280, 833
BlpI	GCTNAGC	1 cut(s)	45
BlsI	GCNGC	7 cut(s)	37, 79, 826, 978, 1005, 1008, 1153
Bme1390I	CCNGG	9 cut(s)	13, 31, 83, 185, 324, 972, 973, 1122, 1143
Bme18I	GGWCC	1 cut(s)	314
BmeRI	GACNNNNNGTC	2 cut(s)	716, 1279
BmeT110I	CYCGRG	2 cut(s)	971, 1091
BmgBI	CACGTC	1 cut(s)	713
BmgT120I	GGNCC	8 cut(s)	33, 239, 314, 412, 413, 968, 969, 1226
BmiI	GGNNCC	8 cut(s)	22, 28, 241, 413, 414, 577, 759, 970
BmrFI	CCNGG	9 cut(s)	13, 31, 83, 185, 324, 972, 973, 1122, 1143
BmrI	ACTGGG	1 cut(s)	622
BmsI	GCATC	3 cut(s)	213, 1021, 1111
BmuI	ACTGGG	1 cut(s)	622
BpiI	GAAGAC	2 cut(s)	803, 908
BpmI	CTGGAG	4 cut(s)	106, 899, 1076, 1118
Bpu10I	CCTNAGC	2 cut(s)	60, 310
Bpu1102I	GCTNAGC	1 cut(s)	45
BpuEI	CTTGAG	2 cut(s)	913, 1196
BpuMI	CCSGG	4 cut(s)	185, 324, 972, 973
BsaBI	GATNNNNATC	1 cut(s)	438
BsaHI	GRCGYC	3 cut(s)	27, 576, 1184
BsaI	GGTCTC	1 cut(s)	209
BsaWI	WCCGGW	1 cut(s)	405
Bse118I	RCCGGY	4 cut(s)	23, 236, 522, 957
Bse1I	ACTGG	1 cut(s)	617
Bse3DI	GCAATG	1 cut(s)	483
Bse8I	GATNNNNATC	1 cut(s)	438
BseBI	CCWGG	5 cut(s)	13, 31, 83, 1122, 1143
BseGI	GGATG	2 cut(s)	647, 1361
BseJI	GATNNNNATC	1 cut(s)	438
BseMI	GCAATG	1 cut(s)	483
BseMII	CTCAG	5 cut(s)	36, 51, 324, 843, 962
BseNI	ACTGG	1 cut(s)	617
BseRI	GAGGAG	3 cut(s)	77, 854, 1073
BseSI	GKGCMC	3 cut(s)	416, 925, 972
BseX3I	CGGCCG	1 cut(s)	1007
BseXI	GCAGC	3 cut(s)	836, 963, 1163
BseYI	CCCAGC	5 cut(s)	104, 551, 580, 696, 1133
BsgI	GTGCAG	1 cut(s)	702
Bsh1236I	CGCG	3 cut(s)	71, 672, 1006
Bsh1285I	CGRYCG	3 cut(s)	277, 406, 1010
BshNI	GGYRCC	3 cut(s)	20, 26, 575
BsiEI	CGRYCG	3 cut(s)	277, 406, 1010
BsiHKAI	GWGCWC	2 cut(s)	69, 949
BsiHKCI	CYCGRG	2 cut(s)	971, 1091
BsiSI	CCGG	9 cut(s)	24, 185, 237, 323, 406, 523, 689, 958, 972
BslFI	GGGAC	3 cut(s)	76, 350, 874
BsmAI	GTCTC	3 cut(s)	209, 345, 1320
BsmBI	CGTCTC	1 cut(s)	345
BsmFI	GGGAC	3 cut(s)	76, 350, 874
Bso31I	GGTCTC	1 cut(s)	209
BsoBI	CYCGRG	2 cut(s)	971, 1091
Bsp120I	GGGCCC	2 cut(s)	412, 968
Bsp1286I	GDGCHC	5 cut(s)	69, 416, 925, 949, 972
Bsp143I	GATC	6 cut(s)	380, 439, 691, 757, 1087, 1249
Bsp1720I	GCTNAGC	1 cut(s)	45
Bsp19I	CCATGG	1 cut(s)	1288
Bsp68I	TCGCGA	1 cut(s)	672
BspCNI	CTCAG	5 cut(s)	37, 52, 323, 844, 961
BspFNI	CGCG	3 cut(s)	71, 672, 1006
BspLI	GGNNCC	8 cut(s)	22, 28, 241, 413, 414, 577, 759, 970
BspMAI	CTGCAG	2 cut(s)	789, 1153
BspMI	ACCTGC	1 cut(s)	778
BspPI	GGATC	5 cut(s)	375, 699, 752, 765, 1244
BspT107I	GGYRCC	3 cut(s)	20, 26, 575
BspTNI	GGTCTC	1 cut(s)	209
BsrBI	CCGCTC	1 cut(s)	1063
BsrDI	GCAATG	1 cut(s)	483
BsrFI	RCCGGY	4 cut(s)	23, 236, 522, 957
BsrI	ACTGG	1 cut(s)	617
BssAI	RCCGGY	4 cut(s)	23, 236, 522, 957
BssMI	GATC	6 cut(s)	380, 439, 691, 757, 1087, 1249
BssNI	GRCGYC	3 cut(s)	27, 576, 1184
BssT1I	CCWWGG	5 cut(s)	243, 280, 833, 1040, 1288
Bst2UI	CCWGG	5 cut(s)	13, 31, 83, 1122, 1143
Bst4CI	ACNGT	2 cut(s)	275, 389
Bst6I	CTCTTC	2 cut(s)	498, 1300
BstACI	GRCGYC	3 cut(s)	27, 576, 1184
BstC8I	GCNNGC	8 cut(s)	25, 51, 69, 416, 533, 633, 911, 1156
BstDEI	CTNAG	5 cut(s)	45, 60, 310, 852, 948
BstDSI	CCRYGG	3 cut(s)	1004, 1253, 1288
BstENI	CCTNNNNNAGG	1 cut(s)	765
BstF5I	GGATG	2 cut(s)	647, 1361
BstFNI	CGCG	3 cut(s)	71, 672, 1006
BstH2I	RGCGCY	4 cut(s)	30, 129, 253, 579
BstHHI	GCGC	5 cut(s)	29, 128, 228, 252, 578
BstKTI	GATC	6 cut(s)	383, 442, 694, 760, 1090, 1252
BstMAI	GTCTC	3 cut(s)	209, 345, 1320
BstMBI	GATC	6 cut(s)	380, 439, 691, 757, 1087, 1249
BstMCI	CGRYCG	3 cut(s)	277, 406, 1010
BstNI	CCWGG	5 cut(s)	13, 31, 83, 1122, 1143
BstSCI	CCNGG	9 cut(s)	11, 29, 81, 183, 322, 970, 971, 1120, 1141
BstSFI	CTRYAG	2 cut(s)	785, 1149
BstSLI	GKGCMC	3 cut(s)	416, 925, 972
BstUI	CGCG	3 cut(s)	71, 672, 1006
BstV1I	GCAGC	3 cut(s)	836, 963, 1163
BstV2I	GAAGAC	2 cut(s)	803, 908
BstX2I	RGATCY	3 cut(s)	380, 757, 1249
BstYI	RGATCY	3 cut(s)	380, 757, 1249
BstZI	CGGCCG	1 cut(s)	1007
BtgI	CCRYGG	3 cut(s)	1004, 1253, 1288
BtrI	CACGTC	1 cut(s)	713
BtsCI	GGATG	2 cut(s)	647, 1361
BtsI	GCAGTG	2 cut(s)	782, 1002
BtsIMutI	CAGTG	2 cut(s)	782, 1002
BtuMI	TCGCGA	1 cut(s)	672
BveI	ACCTGC	1 cut(s)	778
Cac8I	GCNNGC	8 cut(s)	25, 51, 69, 416, 533, 633, 911, 1156
CaiI	CAGNNNCTG	1 cut(s)	1127
CfoI	GCGC	5 cut(s)	29, 128, 228, 252, 578
Cfr10I	RCCGGY	4 cut(s)	23, 236, 522, 957
Cfr13I	GGNCC	8 cut(s)	33, 239, 314, 412, 413, 968, 969, 1226
Cfr42I	CCGCGG	1 cut(s)	1007
Cfr9I	CCCGGG	1 cut(s)	971
CseI	GACGC	1 cut(s)	1192
Csp6I	GTAC	1 cut(s)	271
CviAII	CATG	5 cut(s)	360, 980, 1210, 1289, 1330
CviQI	GTAC	1 cut(s)	271
DdeI	CTNAG	5 cut(s)	45, 60, 310, 852, 948
DinI	GGCGCC	2 cut(s)	28, 577
DpnI	GATC	6 cut(s)	382, 441, 693, 759, 1089, 1251
DpnII	GATC	6 cut(s)	380, 439, 691, 757, 1087, 1249
DriI	GACNNNNNGTC	2 cut(s)	716, 1279
EaeI	YGGCCR	2 cut(s)	3, 1007
EagI	CGGCCG	1 cut(s)	1007
Eam1104I	CTCTTC	2 cut(s)	498, 1300
Eam1105I	GACNNNNNGTC	2 cut(s)	716, 1279
EarI	CTCTTC	2 cut(s)	498, 1300
EciI	GGCGGA	4 cut(s)	363, 470, 723, 1093
Ecl136II	GAGCTC	1 cut(s)	67
EclXI	CGGCCG	1 cut(s)	1007
Eco130I	CCWWGG	5 cut(s)	243, 280, 833, 1040, 1288
Eco147I	AGGCCT	3 cut(s)	937, 1141, 1264
Eco24I	GRGCYC	3 cut(s)	69, 416, 972
Eco31I	GGTCTC	1 cut(s)	209
Eco47I	GGWCC	1 cut(s)	314
Eco47III	AGCGCT	1 cut(s)	251
Eco52I	CGGCCG	1 cut(s)	1007
Eco53kI	GAGCTC	1 cut(s)	67
Eco57I	CTGAAG	2 cut(s)	522, 984
Eco88I	CYCGRG	2 cut(s)	971, 1091
EcoICRI	GAGCTC	1 cut(s)	67
EcoNI	CCTNNNNNAGG	1 cut(s)	765
EcoO109I	RGGNCCY	2 cut(s)	412, 968
EcoRI	GAATTC	1 cut(s)	161
EcoRII	CCWGG	5 cut(s)	11, 29, 81, 1120, 1141
EcoT14I	CCWWGG	5 cut(s)	243, 280, 833, 1040, 1288
EcoT38I	GRGCYC	3 cut(s)	69, 416, 972
EgeI	GGCGCC	2 cut(s)	28, 577
EheI	GGCGCC	2 cut(s)	28, 577
ErhI	CCWWGG	5 cut(s)	243, 280, 833, 1040, 1288
Esp3I	CGTCTC	1 cut(s)	345
FaeI	CATG	5 cut(s)	363, 983, 1213, 1292, 1333
FalI	AAGNNNNNCTT	2 cut(s)	922, 954
FaqI	GGGAC	3 cut(s)	76, 350, 874
FatI	CATG	5 cut(s)	359, 979, 1209, 1288, 1329
FauI	CCCGC	4 cut(s)	281, 326, 423, 1056
FblI	GTMKAC	1 cut(s)	720
Fnu4HI	GCNGC	7 cut(s)	36, 78, 825, 977, 1004, 1007, 1152
FokI	GGATG	1 cut(s)	634
FriOI	GRGCYC	3 cut(s)	69, 416, 972
Fsp4HI	GCNGC	7 cut(s)	36, 78, 825, 977, 1004, 1007, 1152
FspBI	CTAG	7 cut(s)	281, 423, 591, 642, 834, 918, 1203
GlaI	GCGC	5 cut(s)	28, 127, 227, 251, 577
GluI	GCNGC	7 cut(s)	36, 78, 825, 977, 1004, 1007, 1152
GsaI	CCCAGC	5 cut(s)	108, 555, 584, 700, 1137
GsuI	CTGGAG	4 cut(s)	106, 899, 1076, 1118
HaeII	RGCGCY	4 cut(s)	30, 129, 253, 579
HapII	CCGG	9 cut(s)	24, 185, 237, 323, 406, 523, 689, 958, 972
HgaI	GACGC	1 cut(s)	1192
HhaI	GCGC	5 cut(s)	29, 128, 228, 252, 578
Hin1I	GRCGYC	3 cut(s)	27, 576, 1184
Hin1II	CATG	5 cut(s)	363, 983, 1213, 1292, 1333
Hin6I	GCGC	5 cut(s)	27, 126, 226, 250, 576
HinP1I	GCGC	5 cut(s)	27, 126, 226, 250, 576
HincII	GTYRAC	2 cut(s)	466, 721
HindII	GTYRAC	2 cut(s)	466, 721
HinfI	GANTC	6 cut(s)	89, 319, 393, 506, 674, 1199
HpaII	CCGG	9 cut(s)	24, 185, 237, 323, 406, 523, 689, 958, 972
HphI	GGTGA	1 cut(s)	1040
Hpy166II	GTNNAC	3 cut(s)	466, 721, 1018
Hpy188I	TCNGA	8 cut(s)	160, 318, 433, 502, 765, 821, 964, 1198
Hpy188III	TCNNGA	6 cut(s)	212, 671, 1091, 1175, 1203, 1347
Hpy8I	GTNNAC	3 cut(s)	466, 721, 1018
Hpy99I	CGWCG	3 cut(s)	218, 401, 404
HpyAV	CCTTC	8 cut(s)	429, 507, 648, 948, 959, 1197, 1272, 1346
HpyCH4III	ACNGT	2 cut(s)	275, 389
HpyCH4IV	ACGT	1 cut(s)	712
HpyCH4V	TGCA	5 cut(s)	268, 683, 787, 979, 1151
HpyF3I	CTNAG	5 cut(s)	45, 60, 310, 852, 948
HpySE526I	ACGT	1 cut(s)	712
Hsp92I	GRCGYC	3 cut(s)	27, 576, 1184
Hsp92II	CATG	5 cut(s)	363, 983, 1213, 1292, 1333
HspAI	GCGC	5 cut(s)	27, 126, 226, 250, 576
KasI	GGCGCC	2 cut(s)	26, 575
KroI	GCCGGC	1 cut(s)	23
KroNI	GCCGGC	1 cut(s)	25
KspI	CCGCGG	1 cut(s)	1007
Kzo9I	GATC	6 cut(s)	380, 439, 691, 757, 1087, 1249
LmnI	GCTCC	6 cut(s)	64, 247, 640, 821, 1060, 1099
Lsp1109I	GCAGC	3 cut(s)	836, 963, 1163
LweI	GCATC	3 cut(s)	213, 1021, 1111
MaeI	CTAG	7 cut(s)	281, 423, 591, 642, 834, 918, 1203
MaeII	ACGT	1 cut(s)	712
MalI	GATC	6 cut(s)	382, 441, 693, 759, 1089, 1251
MbiI	CCGCTC	1 cut(s)	1063
MboI	GATC	6 cut(s)	380, 439, 691, 757, 1087, 1249
MfeI	CAATTG	1 cut(s)	469
MflI	RGATCY	3 cut(s)	380, 757, 1249
MhlI	GDGCHC	5 cut(s)	69, 416, 925, 949, 972
MlsI	TGGCCA	1 cut(s)	5
MluCI	AATT	4 cut(s)	161, 469, 746, 888
MluNI	TGGCCA	1 cut(s)	5
Mly113I	GGCGCC	2 cut(s)	27, 576
MlyI	GAGTC	1 cut(s)	98
MmeI	TCCRAC	2 cut(s)	735, 864
Mox20I	TGGCCA	1 cut(s)	5
MroNI	GCCGGC	1 cut(s)	23
MscI	TGGCCA	1 cut(s)	5
MslI	CAYNNNNRTG	1 cut(s)	984
Msp20I	TGGCCA	1 cut(s)	5
MspA1I	CMGCKG	1 cut(s)	1006
MspI	CCGG	9 cut(s)	24, 185, 237, 323, 406, 523, 689, 958, 972
MspR9I	CCNGG	9 cut(s)	13, 31, 83, 185, 324, 972, 973, 1122, 1143
MunI	CAATTG	1 cut(s)	469
MvaI	CCWGG	5 cut(s)	13, 31, 83, 1122, 1143
MvnI	CGCG	3 cut(s)	71, 672, 1006
NaeI	GCCGGC	1 cut(s)	25
NarI	GGCGCC	2 cut(s)	27, 576
NciI	CCSGG	4 cut(s)	185, 324, 972, 973
NcoI	CCATGG	1 cut(s)	1288
NdeII	GATC	6 cut(s)	380, 439, 691, 757, 1087, 1249
NgoMIV	GCCGGC	1 cut(s)	23
NlaIII	CATG	5 cut(s)	363, 983, 1213, 1292, 1333
NlaIV	GGNNCC	8 cut(s)	22, 28, 241, 413, 414, 577, 759, 970
NmeAIII	GCCGAG	2 cut(s)	64, 79
NruI	TCGCGA	1 cut(s)	672
PaeR7I	CTCGAG	1 cut(s)	1091
PceI	AGGCCT	3 cut(s)	937, 1141, 1264
PcsI	WCGNNNNNNNCGW	2 cut(s)	393, 911
PdiI	GCCGGC	1 cut(s)	25
PfeI	GAWTC	5 cut(s)	319, 393, 506, 674, 1199
PflMI	CCANNNNNTGG	2 cut(s)	12, 1127
PkrI	GCNGC	7 cut(s)	37, 79, 826, 978, 1005, 1008, 1153
PleI	GAGTC	1 cut(s)	97
PluTI	GGCGCC	2 cut(s)	30, 579
PpsI	GAGTC	1 cut(s)	97
Psp124BI	GAGCTC	1 cut(s)	69
Psp6I	CCWGG	5 cut(s)	11, 29, 81, 1120, 1141
PspFI	CCCAGC	5 cut(s)	104, 551, 580, 696, 1133
PspGI	CCWGG	5 cut(s)	11, 29, 81, 1120, 1141
PspN4I	GGNNCC	8 cut(s)	22, 28, 241, 413, 414, 577, 759, 970
PspOMI	GGGCCC	2 cut(s)	412, 968
PspPI	GGNCC	8 cut(s)	33, 239, 314, 412, 413, 968, 969, 1226
PstI	CTGCAG	2 cut(s)	789, 1153
PstNI	CAGNNNCTG	1 cut(s)	1127
PsuI	RGATCY	3 cut(s)	380, 757, 1249
RruI	TCGCGA	1 cut(s)	672
RsaI	GTAC	1 cut(s)	272
RsaNI	GTAC	1 cut(s)	271
RseI	CAYNNNNRTG	1 cut(s)	984
SacI	GAGCTC	1 cut(s)	69
SacII	CCGCGG	1 cut(s)	1007
SalI	GTCGAC	1 cut(s)	719
SatI	GCNGC	7 cut(s)	36, 78, 825, 977, 1004, 1007, 1152
Sau3AI	GATC	6 cut(s)	380, 439, 691, 757, 1087, 1249
Sau96I	GGNCC	8 cut(s)	33, 239, 314, 412, 413, 968, 969, 1226
SchI	GAGTC	1 cut(s)	98
ScrFI	CCNGG	9 cut(s)	13, 31, 83, 185, 324, 972, 973, 1122, 1143
SduI	GDGCHC	5 cut(s)	69, 416, 925, 949, 972
SfaNI	GCATC	3 cut(s)	213, 1021, 1111
SfcI	CTRYAG	2 cut(s)	785, 1149
SfoI	GGCGCC	2 cut(s)	28, 577
Sfr274I	CTCGAG	1 cut(s)	1091
Sfr303I	CCGCGG	1 cut(s)	1007
SgrBI	CCGCGG	1 cut(s)	1007
SinI	GGWCC	1 cut(s)	314
SlaI	CTCGAG	1 cut(s)	1091
SmaI	CCCGGG	1 cut(s)	973
SmiMI	CAYNNNNRTG	1 cut(s)	984
SmlI	CTYRAG	3 cut(s)	928, 1091, 1175
SmoI	CTYRAG	3 cut(s)	928, 1091, 1175
SpeI	ACTAGT	1 cut(s)	917
SrfI	GCCCGGGC	1 cut(s)	973
Sse9I	AATT	4 cut(s)	161, 469, 746, 888
SseBI	AGGCCT	3 cut(s)	937, 1141, 1264
SspDI	GGCGCC	2 cut(s)	26, 575
SspMI	CTAG	7 cut(s)	281, 423, 591, 642, 834, 918, 1203
SstI	GAGCTC	1 cut(s)	69
StuI	AGGCCT	3 cut(s)	937, 1141, 1264
StyD4I	CCNGG	9 cut(s)	11, 29, 81, 183, 322, 970, 971, 1120, 1141
StyI	CCWWGG	5 cut(s)	243, 280, 833, 1040, 1288
TaaI	ACNGT	2 cut(s)	275, 389
TaiI	ACGT	1 cut(s)	715
TaqI	TCGA	8 cut(s)	87, 396, 438, 509, 720, 829, 941, 1092
TaqII	GACCGA	1 cut(s)	191
TasI	AATT	4 cut(s)	161, 469, 746, 888
TauI	GCSGC	4 cut(s)	38, 80, 1006, 1009
TfiI	GAWTC	5 cut(s)	319, 393, 506, 674, 1199
TscAI	CASTG	2 cut(s)	789, 1002
TseI	GCWGC	3 cut(s)	824, 976, 1151
TspGWI	ACGGA	3 cut(s)	354, 878, 1242
TspMI	CCCGGG	1 cut(s)	971
TspRI	CASTG	2 cut(s)	789, 1002
Van91I	CCANNNNNTGG	2 cut(s)	12, 1127
VpaK11BI	GGWCC	1 cut(s)	314
XagI	CCTNNNNNAGG	1 cut(s)	765
XapI	RAATTY	1 cut(s)	161
XbaI	TCTAGA	1 cut(s)	1202
XhoI	CTCGAG	1 cut(s)	1091
XmaI	CCCGGG	1 cut(s)	971
XmaJI	CCTAGG	2 cut(s)	280, 833
XmiI	GTMKAC	1 cut(s)	720
XspI	CTAG	7 cut(s)	281, 423, 591, 642, 834, 918, 1203

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.