Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0014248

Physical Location & Seq

Genomic Coordinates Forward (+)

6932739 .. 6936142

Gene Structure 3404 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1099600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1638 bp

ATGGCCAAACCCTGGGGTACTGTCGGCAACTGGGCCGCCGAGGCTGAGCGAGAAGAGGCCGAGGAGCTCGCGGCGGCCGCCAAGGCCGAGTCCCAGAGCTTTCCCAGTCTAAAGGAAGCCGTCAGCGCCAAGCCCAAGAAGAAGAAGATGAGCCTCTCTGAATTCTACAACGCCTCCGGCACCCCCGCCTCTAATCGTGTCGGCCTCACTCCCGACGAGATGATGCGCCTCCCTACCGGCCCCAAGGAGCGCTCTGCCGAAGAAATGCAGTACGGTCGCCTCGGTGGCGGGTTTCCCTCCTACGGTCGCTCTGGTACGAATCCGGGTCGGGGCGGGGACAGAGAGGGCGGGGACGGGTCGTGGGGTGGTGGAGGAAGAAGATCCTACGGTGGATTCGACGACGACAGGAGGGGCCCGCCTTCTAGGGTTTCTGATTTAGATCAACCGTCAAGGGCGGACGAGGTTGACAATTGGGCAATGACTAAAAAGTCTCTTCCTTCTGTAGATTCCGGTAGGCAGAACCGGTATAGCTCGCTCGGTAGTGTTGGTGCTGGGGGTGGGGGTGGTGATGGTGTAGGTGGTGGTATGGGCGGCCCAGCTTGGTCTAGGGCAGACGAGGTTGATAACTGGGCTGTTGGAAAAAAGCCTACTCCTTCTAGGTCATCCCCATTTGGGTCTGAATTTCATTCGGGTCCGGAACCGGATCGCTGGGTGAGAGGCGGACGCGAGGTCGACAGAGTTGAGAGAGAGCGACCCAAATTGGTTTTGGATCCTCCGAAAGCCGAGTCCGGCGTGAATGAGCCATCGCAGGTTGTGAAGACCAATAAGCCGAACCCCTTCGGAGCAGCTCGACCTAGGGAGGAGATTTTGGCTGAGAAAGGGTTGGATTGGAAGAAGTTGGAGTCGGAAATAGAAGCCAAGAAGACGAGTAGGCCGACGAGTGCCCACTCAAGCAGGCCTTCGAGTGCTCAGTCAAGCCGGTCTGAAGGGCCCGGGCTGCATGCAAGTGATAATGTGGTGAAGCCGCGGCCGAAAGTAAACCCCTTTGGTGATGCCAAGCCAAGGGAGGTTTTGCTGGAGGAGCGGGGTAAGGATTGGCGGAAGATTGATCTCGAGCTGGAGCATCAGGGTATTGACAGACCTGAAACAGAGGAGGAAAAGATATTGAAGGAAGAAATAGATCATCTAAAGAAGGAACTCGAGAAAGAATCTACGGATAAAGTGAACAGTGAAACTGTGCAAGAGTCTGGTGGTGACCGGCCAAGTCTACGCGATATAATACTACAGAAGGAAAGGGAATTGGAGGCATTGATCCGAGAATTTGATGACAAAGTTCGCTTTGGGCAGAAGGCCATCGATAGGCCAGGGTCTGGGGCTGGTAGGCCTGGTTCTGGGGCAGGAAGGCCTGGGTCTTCAGCAGGGAGGGCTGGTAACTTTCATGAGAGGACGCCGTCTTATTCCGGGTCATTTGAAGATTCTAGAAGTGTGGAATACGTAGATAGGTCTCCCTTGCAAGGCAAGGGAGATCCGTGGGCAAGGCCTTTTGATGACAGAAGGTCATTCCATGGTGGCAGGGAAAGAGGAGGATTTCTGGGGAGCAGAGACATGGGCAGGGCAAGGTCAAGAGAAAGATGGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0003743 GO:0005488 GO:0005515 GO:0005575 GO:0005618 GO:0005623 GO:0005911 GO:0006412 GO:0006413 GO:0006518 GO:0006807 GO:0008135 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009505 GO:0009506 GO:0009987 GO:0010467 GO:0019538 GO:0030054 GO:0030312 GO:0034641 GO:0034645 GO:0042802 GO:0042803 GO:0043043 GO:0043170 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044464 GO:0046983 GO:0055044 GO:0071704 GO:0071944 GO:0097159 GO:1901363 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

eIF-4B

Protein Analysis

546

Amino Acids

59.4

Weight (kDa)

8.39

Isoelectric Point (pI)

52.47

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
eIF-4B	PF06273	1 - 464	2.9e-212	Plant specific eukaryotic initiation factor 4B
eIF-4B	PF06273	467 - 528	2.7e-17	Plant specific eukaryotic initiation factor 4B

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0014248)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G13020
fragaria_vesca	FvH4_4g26000
malus_domestica	MD13G1098600.v1.1 MD16G1099600.v1.1
prunus_persica	Prupe.1G246800_v2.0.a1 Prupe.1G246800_v2.0.a1
pyrus_communis	pycom13g08630 pycom16g08470
rosa_chinensis	RchiOBHm_Chr4g0433421
rosa_laevigata	RLG00000006754
rosa_roxburghii	Rroxscaffold_5G00374970
rosa_rugosa	Rorug04G0268700
rosa_samantha	Rh4AG323400 Rh4BG331300 Rh4CG346600 Rh4DG327600
rosa_wichuraiana	Rw4G028020

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	487
Acc36I	ACCTGC	1 cut(s)	799
AccB1I	GGYRCC	1 cut(s)	179
AccB7I	CCANNNNNTGG	2 cut(s)	12, 1370
AccBSI	CCGCTC	1 cut(s)	1084
AccI	GTMKAC	2 cut(s)	732, 1267
AccII	CGCG	4 cut(s)	71, 726, 1027, 1272
AccIII	TCCGGA	1 cut(s)	694
AclWI	GGATC	6 cut(s)	375, 711, 764, 777, 1306, 1520
AcoI	YGGCCR	4 cut(s)	3, 75, 1028, 1259
AcsI	RAATTY	3 cut(s)	161, 680, 1319
AcuI	CTGAAG	2 cut(s)	1005, 1398
AcyI	GRCGYC	1 cut(s)	1448
AfaI	GTAC	3 cut(s)	19, 272, 316
AfeI	AGCGCT	1 cut(s)	251
AfiI	CCNNNNNNNGG	9 cut(s)	12, 302, 329, 672, 1062, 1359, 1370, 1391, 1514
AgeI	ACCGGT	1 cut(s)	522
AgsI	TTSAA	2 cut(s)	1168, 1472
AhdI	GACNNNNNGTC	2 cut(s)	728, 1555
AjnI	CCWGG	4 cut(s)	11, 1363, 1384, 1405
AluBI	AGCT	6 cut(s)	67, 99, 531, 599, 848, 1117
AluI	AGCT	6 cut(s)	67, 99, 531, 599, 848, 1117
Alw21I	GWGCWC	2 cut(s)	69, 970
Alw26I	GTCTC	3 cut(s)	495, 1509, 1596
AlwI	GGATC	6 cut(s)	375, 711, 764, 777, 1306, 1520
AlwNI	CAGNNNCTG	1 cut(s)	1370
Ama87I	CYCGRG	3 cut(s)	992, 1112, 1199
Aor13HI	TCCGGA	1 cut(s)	694
Aor51HI	AGCGCT	1 cut(s)	251
ApaI	GGGCCC	2 cut(s)	416, 993
ApeKI	GCWGC	2 cut(s)	845, 997
ApoI	RAATTY	3 cut(s)	161, 680, 1319
ArsI	GACNNNNNNTTYG	2 cut(s)	770, 802
AsiGI	ACCGGT	1 cut(s)	522
Asp700I	GAANNNNTTC	1 cut(s)	836
AspA2I	CCTAGG	1 cut(s)	854
AspLEI	GCGC	3 cut(s)	128, 228, 252
AspS9I	GGNCC	8 cut(s)	33, 239, 412, 413, 593, 692, 989, 990
AsuC2I	CCSGG	4 cut(s)	324, 993, 994, 1462
AsuHPI	GGTGA	5 cut(s)	578, 724, 1030, 1061, 1265
AvaI	CYCGRG	3 cut(s)	992, 1112, 1199
AvaII	GGWCC	1 cut(s)	692
AvrII	CCTAGG	1 cut(s)	854
BaeGI	GKGCMC	3 cut(s)	416, 946, 993
BalI	TGGCCA	1 cut(s)	5
BamHI	GGATCC	1 cut(s)	769
BanI	GGYRCC	1 cut(s)	179
BanII	GRGCYC	3 cut(s)	69, 416, 993
BbsI	GAAGAC	3 cut(s)	824, 929, 1404
Bbv12I	GWGCWC	2 cut(s)	69, 970
BbvI	GCAGC	2 cut(s)	857, 984
BccI	CCATC	4 cut(s)	563, 811, 1361, 1626
BceAI	ACGGC	2 cut(s)	104, 1435
BcgI	CGANNNNNNTGC	2 cut(s)	257, 291
BciT130I	CCWGG	4 cut(s)	13, 1365, 1386, 1407
BcnI	CCSGG	4 cut(s)	324, 993, 994, 1462
BcoDI	GTCTC	3 cut(s)	495, 1509, 1596
BfaI	CTAG	5 cut(s)	423, 606, 657, 855, 1479
BfmI	CTRYAG	2 cut(s)	501, 1283
BfoI	RGCGCY	2 cut(s)	129, 253
BfuAI	ACCTGC	1 cut(s)	799
BglI	GCCNNNNNGGC	3 cut(s)	41, 83, 285
BisI	GCNGC	9 cut(s)	36, 72, 75, 78, 592, 846, 998, 1025, 1028
BlnI	CCTAGG	1 cut(s)	854
BlpI	GCTNAGC	1 cut(s)	45
BlsI	GCNGC	9 cut(s)	37, 73, 76, 79, 593, 847, 999, 1026, 1029
Bme1390I	CCNGG	8 cut(s)	13, 324, 993, 994, 1365, 1386, 1407, 1462
Bme18I	GGWCC	1 cut(s)	692
BmeRI	GACNNNNNGTC	2 cut(s)	728, 1555
BmeT110I	CYCGRG	3 cut(s)	992, 1112, 1199
BmgT120I	GGNCC	8 cut(s)	33, 239, 412, 413, 593, 692, 989, 990
BmiI	GGNNCC	8 cut(s)	181, 241, 413, 414, 693, 699, 771, 991
BmrFI	CCNGG	8 cut(s)	13, 324, 993, 994, 1365, 1386, 1407, 1462
BmrI	ACTGGG	3 cut(s)	40, 99, 637
BmsI	GCATC	3 cut(s)	213, 1042, 1132
BmuI	ACTGGG	3 cut(s)	40, 99, 637
BpiI	GAAGAC	3 cut(s)	824, 929, 1404
BpmI	CTGGAG	2 cut(s)	1097, 1139
Bpu1102I	GCTNAGC	1 cut(s)	45
BpuEI	CTTGAG	1 cut(s)	934
BpuMI	CCSGG	4 cut(s)	324, 993, 994, 1462
Bsa29I	ATCGAT	1 cut(s)	1356
BsaAI	YACGTR	1 cut(s)	1495
BsaBI	GATNNNNATC	1 cut(s)	438
BsaHI	GRCGYC	1 cut(s)	1448
BsaI	GGTCTC	1 cut(s)	1509
BsaWI	WCCGGW	4 cut(s)	509, 522, 694, 700
BsaXI	ACNNNNNCTCC	4 cut(s)	158, 188, 1414, 1444
Bsc4I	CCNNNNNNNGG	9 cut(s)	12, 302, 329, 672, 1062, 1359, 1370, 1391, 1514
Bse118I	RCCGGY	4 cut(s)	236, 522, 978, 1257
Bse1I	ACTGG	3 cut(s)	35, 105, 632
Bse3DI	GCAATG	1 cut(s)	483
Bse8I	GATNNNNATC	1 cut(s)	438
BseAI	TCCGGA	1 cut(s)	694
BseBI	CCWGG	4 cut(s)	13, 1365, 1386, 1407
BseCI	ATCGAT	1 cut(s)	1356
BseGI	GGATG	1 cut(s)	662
BseJI	GATNNNNATC	1 cut(s)	438
BseLI	CCNNNNNNNGG	9 cut(s)	12, 302, 329, 672, 1062, 1359, 1370, 1391, 1514
BseMI	GCAATG	1 cut(s)	483
BseMII	CTCAG	3 cut(s)	36, 864, 983
BseNI	ACTGG	3 cut(s)	35, 105, 632
BseRI	GAGGAG	5 cut(s)	77, 875, 1094, 1166, 1596
BseSI	GKGCMC	3 cut(s)	416, 946, 993
BseX3I	CGGCCG	2 cut(s)	75, 1028
BseXI	GCAGC	2 cut(s)	857, 984
BseYI	CCCAGC	3 cut(s)	551, 595, 708
Bsh1236I	CGCG	4 cut(s)	71, 726, 1027, 1272
Bsh1285I	CGRYCG	4 cut(s)	78, 277, 307, 1031
BshNI	GGYRCC	1 cut(s)	179
BshTI	ACCGGT	1 cut(s)	522
BshVI	ATCGAT	1 cut(s)	1356
BsiEI	CGRYCG	4 cut(s)	78, 277, 307, 1031
BsiHKAI	GWGCWC	2 cut(s)	69, 970
BsiHKCI	CYCGRG	3 cut(s)	992, 1112, 1199
BslFI	GGGAC	3 cut(s)	76, 350, 365
BslI	CCNNNNNNNGG	9 cut(s)	12, 302, 329, 672, 1062, 1359, 1370, 1391, 1514
BsmAI	GTCTC	3 cut(s)	495, 1509, 1596
BsmFI	GGGAC	3 cut(s)	76, 350, 365
Bso31I	GGTCTC	1 cut(s)	1509
BsoBI	CYCGRG	3 cut(s)	992, 1112, 1199
Bsp120I	GGGCCC	2 cut(s)	412, 989
Bsp1286I	GDGCHC	5 cut(s)	69, 416, 946, 970, 993
Bsp13I	TCCGGA	1 cut(s)	694
Bsp143I	GATC	8 cut(s)	380, 439, 703, 769, 1108, 1180, 1311, 1525
Bsp1720I	GCTNAGC	1 cut(s)	45
Bsp19I	CCATGG	1 cut(s)	1564
BspCNI	CTCAG	3 cut(s)	37, 865, 982
BspDI	ATCGAT	1 cut(s)	1356
BspEI	TCCGGA	1 cut(s)	694
BspFNI	CGCG	4 cut(s)	71, 726, 1027, 1272
BspHI	TCATGA	1 cut(s)	1438
BspLI	GGNNCC	8 cut(s)	181, 241, 413, 414, 693, 699, 771, 991
BspMI	ACCTGC	1 cut(s)	799
BspPI	GGATC	6 cut(s)	375, 711, 764, 777, 1306, 1520
BspT107I	GGYRCC	1 cut(s)	179
BspTNI	GGTCTC	1 cut(s)	1509
BsrBI	CCGCTC	1 cut(s)	1084
BsrDI	GCAATG	1 cut(s)	483
BsrFI	RCCGGY	4 cut(s)	236, 522, 978, 1257
BsrI	ACTGG	3 cut(s)	35, 105, 632
BssAI	RCCGGY	4 cut(s)	236, 522, 978, 1257
BssMI	GATC	8 cut(s)	380, 439, 703, 769, 1108, 1180, 1311, 1525
BssNI	GRCGYC	1 cut(s)	1448
BssT1I	CCWWGG	5 cut(s)	81, 243, 854, 1061, 1564
Bst2UI	CCWGG	4 cut(s)	13, 1365, 1386, 1407
Bst4CI	ACNGT	7 cut(s)	22, 275, 305, 389, 447, 1229, 1237
Bst6I	CTCTTC	2 cut(s)	48, 498
BstACI	GRCGYC	1 cut(s)	1448
BstBAI	YACGTR	1 cut(s)	1495
BstC8I	GCNNGC	5 cut(s)	69, 416, 533, 956, 1002
BstDEI	CTNAG	3 cut(s)	45, 873, 969
BstDSI	CCRYGG	3 cut(s)	1025, 1529, 1564
BstEII	GGTNACC	1 cut(s)	1253
BstF5I	GGATG	1 cut(s)	662
BstFNI	CGCG	4 cut(s)	71, 726, 1027, 1272
BstH2I	RGCGCY	2 cut(s)	129, 253
BstHHI	GCGC	3 cut(s)	128, 228, 252
BstKTI	GATC	8 cut(s)	383, 442, 706, 772, 1111, 1183, 1314, 1528
BstMAI	GTCTC	3 cut(s)	495, 1509, 1596
BstMBI	GATC	8 cut(s)	380, 439, 703, 769, 1108, 1180, 1311, 1525
BstMCI	CGRYCG	4 cut(s)	78, 277, 307, 1031
BstMWI	GCNNNNNNNGC	8 cut(s)	41, 77, 83, 125, 285, 997, 1081, 1424
BstNI	CCWGG	4 cut(s)	13, 1365, 1386, 1407
BstNSI	RCATGY	1 cut(s)	1004
BstPI	GGTNACC	1 cut(s)	1253
BstSCI	CCNGG	8 cut(s)	11, 322, 991, 992, 1363, 1384, 1405, 1460
BstSFI	CTRYAG	2 cut(s)	501, 1283
BstSLI	GKGCMC	3 cut(s)	416, 946, 993
BstSNI	TACGTA	1 cut(s)	1495
BstUI	CGCG	4 cut(s)	71, 726, 1027, 1272
BstV1I	GCAGC	2 cut(s)	857, 984
BstV2I	GAAGAC	3 cut(s)	824, 929, 1404
BstX2I	RGATCY	3 cut(s)	380, 769, 1525
BstYI	RGATCY	3 cut(s)	380, 769, 1525
BstZI	CGGCCG	2 cut(s)	75, 1028
Bsu15I	ATCGAT	1 cut(s)	1356
BsuTUI	ATCGAT	1 cut(s)	1356
BtgI	CCRYGG	3 cut(s)	1025, 1529, 1564
BtgZI	GCGATG	1 cut(s)	789
BtsCI	GGATG	1 cut(s)	662
BtsIMutI	CAGTG	1 cut(s)	1234
BveI	ACCTGC	1 cut(s)	799
Cac8I	GCNNGC	5 cut(s)	69, 416, 533, 956, 1002
CaiI	CAGNNNCTG	1 cut(s)	1370
CciI	TCATGA	1 cut(s)	1438
CciNI	GCGGCCGC	1 cut(s)	75
CfoI	GCGC	3 cut(s)	128, 228, 252
Cfr10I	RCCGGY	4 cut(s)	236, 522, 978, 1257
Cfr13I	GGNCC	8 cut(s)	33, 239, 412, 413, 593, 692, 989, 990
Cfr42I	CCGCGG	1 cut(s)	1028
Cfr9I	CCCGGG	1 cut(s)	992
ClaI	ATCGAT	1 cut(s)	1356
CseI	GACGC	2 cut(s)	732, 1456
Csp6I	GTAC	3 cut(s)	18, 271, 315
CspAI	ACCGGT	1 cut(s)	522
CviAII	CATG	4 cut(s)	1001, 1439, 1565, 1606
CviQI	GTAC	3 cut(s)	18, 271, 315
DdeI	CTNAG	3 cut(s)	45, 873, 969
DpnI	GATC	8 cut(s)	382, 441, 705, 771, 1110, 1182, 1313, 1527
DpnII	GATC	8 cut(s)	380, 439, 703, 769, 1108, 1180, 1311, 1525
DrdI	GACNNNNNNGTC	1 cut(s)	487
DriI	GACNNNNNGTC	2 cut(s)	728, 1555
DseDI	GACNNNNNNGTC	1 cut(s)	487
EaeI	YGGCCR	4 cut(s)	3, 75, 1028, 1259
EagI	CGGCCG	2 cut(s)	75, 1028
Eam1104I	CTCTTC	2 cut(s)	48, 498
Eam1105I	GACNNNNNGTC	2 cut(s)	728, 1555
EarI	CTCTTC	2 cut(s)	48, 498
EciI	GGCGGA	3 cut(s)	470, 735, 1114
Ecl136II	GAGCTC	1 cut(s)	67
EclXI	CGGCCG	2 cut(s)	75, 1028
Eco105I	TACGTA	1 cut(s)	1495
Eco130I	CCWWGG	5 cut(s)	81, 243, 854, 1061, 1564
Eco147I	AGGCCT	4 cut(s)	958, 1384, 1405, 1540
Eco24I	GRGCYC	3 cut(s)	69, 416, 993
Eco31I	GGTCTC	1 cut(s)	1509
Eco47I	GGWCC	1 cut(s)	692
Eco47III	AGCGCT	1 cut(s)	251
Eco52I	CGGCCG	2 cut(s)	75, 1028
Eco53kI	GAGCTC	1 cut(s)	67
Eco57I	CTGAAG	2 cut(s)	1005, 1398
Eco88I	CYCGRG	3 cut(s)	992, 1112, 1199
Eco91I	GGTNACC	1 cut(s)	1253
EcoICRI	GAGCTC	1 cut(s)	67
EcoO109I	RGGNCCY	2 cut(s)	412, 989
EcoO65I	GGTNACC	1 cut(s)	1253
EcoRI	GAATTC	1 cut(s)	161
EcoRII	CCWGG	4 cut(s)	11, 1363, 1384, 1405
EcoT14I	CCWWGG	5 cut(s)	81, 243, 854, 1061, 1564
EcoT38I	GRGCYC	3 cut(s)	69, 416, 993
ErhI	CCWWGG	5 cut(s)	81, 243, 854, 1061, 1564
FaeI	CATG	4 cut(s)	1004, 1442, 1568, 1609
FaiI	YATR	7 cut(s)	528, 587, 1002, 1277, 1440, 1566, 1607
FalI	AAGNNNNNCTT	2 cut(s)	943, 975
FaqI	GGGAC	3 cut(s)	76, 350, 365
FatI	CATG	4 cut(s)	1000, 1438, 1564, 1605
FauI	CCCGC	6 cut(s)	193, 281, 326, 341, 423, 1077
FblI	GTMKAC	2 cut(s)	732, 1267
Fnu4HI	GCNGC	9 cut(s)	36, 72, 75, 78, 592, 846, 998, 1025, 1028
FokI	GGATG	1 cut(s)	649
FriOI	GRGCYC	3 cut(s)	69, 416, 993
Fsp4HI	GCNGC	9 cut(s)	36, 72, 75, 78, 592, 846, 998, 1025, 1028
FspBI	CTAG	5 cut(s)	423, 606, 657, 855, 1479
GlaI	GCGC	3 cut(s)	127, 227, 251
GluI	GCNGC	9 cut(s)	36, 72, 75, 78, 592, 846, 998, 1025, 1028
GsaI	CCCAGC	3 cut(s)	555, 599, 712
GsuI	CTGGAG	2 cut(s)	1097, 1139
HaeII	RGCGCY	2 cut(s)	129, 253
HgaI	GACGC	2 cut(s)	732, 1456
HhaI	GCGC	3 cut(s)	128, 228, 252
Hin1I	GRCGYC	1 cut(s)	1448
Hin1II	CATG	4 cut(s)	1004, 1442, 1568, 1609
Hin6I	GCGC	3 cut(s)	126, 226, 250
HinP1I	GCGC	3 cut(s)	126, 226, 250
HincII	GTYRAC	2 cut(s)	466, 733
HindII	GTYRAC	2 cut(s)	466, 733
HinfI	GANTC	9 cut(s)	89, 319, 393, 506, 785, 902, 1208, 1244, 1475
HphI	GGTGA	5 cut(s)	578, 724, 1030, 1061, 1265
Hpy166II	GTNNAC	5 cut(s)	466, 733, 1039, 1225, 1268
Hpy188I	TCNGA	8 cut(s)	160, 433, 679, 777, 842, 907, 985, 1316
Hpy188III	TCNNGA	7 cut(s)	212, 695, 1112, 1201, 1439, 1479, 1623
Hpy8I	GTNNAC	5 cut(s)	466, 733, 1039, 1225, 1268
Hpy99I	CGWCG	4 cut(s)	218, 401, 404, 940
HpyCH4III	ACNGT	7 cut(s)	22, 275, 305, 389, 447, 1229, 1237
HpyCH4IV	ACGT	1 cut(s)	1494
HpyCH4V	TGCA	5 cut(s)	268, 1000, 1004, 1240, 1513
HpyF10VI	GCNNNNNNNGC	8 cut(s)	41, 77, 83, 125, 285, 997, 1081, 1424
HpyF3I	CTNAG	3 cut(s)	45, 873, 969
HpySE526I	ACGT	1 cut(s)	1494
Hsp92I	GRCGYC	1 cut(s)	1448
Hsp92II	CATG	4 cut(s)	1004, 1442, 1568, 1609
HspAI	GCGC	3 cut(s)	126, 226, 250
Kpn2I	TCCGGA	1 cut(s)	694
KspI	CCGCGG	1 cut(s)	1028
Kzo9I	GATC	8 cut(s)	380, 439, 703, 769, 1108, 1180, 1311, 1525
LmnI	GCTCC	6 cut(s)	64, 247, 842, 1081, 1120, 1596
Lsp1109I	GCAGC	2 cut(s)	857, 984
LweI	GCATC	3 cut(s)	213, 1042, 1132
MaeI	CTAG	5 cut(s)	423, 606, 657, 855, 1479
MaeII	ACGT	1 cut(s)	1494
MaeIII	GTNAC	2 cut(s)	1253, 1430
MalI	GATC	8 cut(s)	382, 441, 705, 771, 1110, 1182, 1313, 1527
MbiI	CCGCTC	1 cut(s)	1084
MboI	GATC	8 cut(s)	380, 439, 703, 769, 1108, 1180, 1311, 1525
MfeI	CAATTG	1 cut(s)	469
MflI	RGATCY	3 cut(s)	380, 769, 1525
MhlI	GDGCHC	5 cut(s)	69, 416, 946, 970, 993
MlsI	TGGCCA	1 cut(s)	5
MluCI	AATT	6 cut(s)	161, 469, 680, 758, 1298, 1319
MluNI	TGGCCA	1 cut(s)	5
MlyI	GAGTC	4 cut(s)	98, 794, 911, 1253
MmeI	TCCRAC	4 cut(s)	616, 864, 879, 885
Mox20I	TGGCCA	1 cut(s)	5
MroI	TCCGGA	1 cut(s)	694
MroXI	GAANNNNTTC	1 cut(s)	836
MscI	TGGCCA	1 cut(s)	5
MslI	CAYNNNNRTG	1 cut(s)	1005
Msp20I	TGGCCA	1 cut(s)	5
MspA1I	CMGCKG	1 cut(s)	1027
MspR9I	CCNGG	8 cut(s)	13, 324, 993, 994, 1365, 1386, 1407, 1462
MunI	CAATTG	1 cut(s)	469
MvaI	CCWGG	4 cut(s)	13, 1365, 1386, 1407
MvnI	CGCG	4 cut(s)	71, 726, 1027, 1272
MwoI	GCNNNNNNNGC	8 cut(s)	41, 77, 83, 125, 285, 997, 1081, 1424
NciI	CCSGG	4 cut(s)	324, 993, 994, 1462
NcoI	CCATGG	1 cut(s)	1564
NdeII	GATC	8 cut(s)	380, 439, 703, 769, 1108, 1180, 1311, 1525
NlaIII	CATG	4 cut(s)	1004, 1442, 1568, 1609
NlaIV	GGNNCC	8 cut(s)	181, 241, 413, 414, 693, 699, 771, 991
NmeAIII	GCCGAG	4 cut(s)	64, 85, 112, 808
NmuCI	GTSAC	1 cut(s)	1253
NotI	GCGGCCGC	1 cut(s)	75
NspI	RCATGY	1 cut(s)	1004
PaeI	GCATGC	1 cut(s)	1004
PaeR7I	CTCGAG	2 cut(s)	1112, 1199
PagI	TCATGA	1 cut(s)	1438
PasI	CCCWGGG	1 cut(s)	12
PceI	AGGCCT	4 cut(s)	958, 1384, 1405, 1540
PcsI	WCGNNNNNNNCGW	2 cut(s)	393, 932
PdmI	GAANNNNTTC	1 cut(s)	836
PfeI	GAWTC	5 cut(s)	319, 393, 506, 1208, 1475
PflFI	GACNNNGTC	1 cut(s)	1450
PflMI	CCANNNNNTGG	2 cut(s)	12, 1370
PinAI	ACCGGT	1 cut(s)	522
PkrI	GCNGC	9 cut(s)	37, 73, 76, 79, 593, 847, 999, 1026, 1029
PleI	GAGTC	4 cut(s)	97, 793, 910, 1252
PpsI	GAGTC	4 cut(s)	97, 793, 910, 1252
Ppu21I	YACGTR	1 cut(s)	1495
Psp124BI	GAGCTC	1 cut(s)	69
Psp6I	CCWGG	4 cut(s)	11, 1363, 1384, 1405
PspEI	GGTNACC	1 cut(s)	1253
PspFI	CCCAGC	3 cut(s)	551, 595, 708
PspGI	CCWGG	4 cut(s)	11, 1363, 1384, 1405
PspN4I	GGNNCC	8 cut(s)	181, 241, 413, 414, 693, 699, 771, 991
PspOMI	GGGCCC	2 cut(s)	412, 989
PspPI	GGNCC	8 cut(s)	33, 239, 412, 413, 593, 692, 989, 990
PsrI	GAACNNNNNNTAC	2 cut(s)	1372, 1404
PstNI	CAGNNNCTG	1 cut(s)	1370
PsuI	RGATCY	3 cut(s)	380, 769, 1525
PsyI	GACNNNGTC	1 cut(s)	1450
RsaI	GTAC	3 cut(s)	19, 272, 316
RsaNI	GTAC	3 cut(s)	18, 271, 315
RseI	CAYNNNNRTG	1 cut(s)	1005
SacI	GAGCTC	1 cut(s)	69
SacII	CCGCGG	1 cut(s)	1028
SalI	GTCGAC	1 cut(s)	731
SatI	GCNGC	9 cut(s)	36, 72, 75, 78, 592, 846, 998, 1025, 1028
Sau3AI	GATC	8 cut(s)	380, 439, 703, 769, 1108, 1180, 1311, 1525
Sau96I	GGNCC	8 cut(s)	33, 239, 412, 413, 593, 692, 989, 990
SchI	GAGTC	4 cut(s)	98, 794, 911, 1253
ScrFI	CCNGG	8 cut(s)	13, 324, 993, 994, 1365, 1386, 1407, 1462
SduI	GDGCHC	5 cut(s)	69, 416, 946, 970, 993
SfaNI	GCATC	3 cut(s)	213, 1042, 1132
SfcI	CTRYAG	2 cut(s)	501, 1283
SfiI	GGCCNNNNNGGCC	1 cut(s)	83
Sfr274I	CTCGAG	2 cut(s)	1112, 1199
Sfr303I	CCGCGG	1 cut(s)	1028
SgrBI	CCGCGG	1 cut(s)	1028
SinI	GGWCC	1 cut(s)	692
SlaI	CTCGAG	2 cut(s)	1112, 1199
SmaI	CCCGGG	1 cut(s)	994
SmiMI	CAYNNNNRTG	1 cut(s)	1005
SmlI	CTYRAG	3 cut(s)	949, 1112, 1199
SmoI	CTYRAG	3 cut(s)	949, 1112, 1199
SnaBI	TACGTA	1 cut(s)	1495
SphI	GCATGC	1 cut(s)	1004
SrfI	GCCCGGGC	1 cut(s)	994
Sse9I	AATT	6 cut(s)	161, 469, 680, 758, 1298, 1319
SseBI	AGGCCT	4 cut(s)	958, 1384, 1405, 1540
SspMI	CTAG	5 cut(s)	423, 606, 657, 855, 1479
SstI	GAGCTC	1 cut(s)	69
StuI	AGGCCT	4 cut(s)	958, 1384, 1405, 1540
StyD4I	CCNGG	8 cut(s)	11, 322, 991, 992, 1363, 1384, 1405, 1460
StyI	CCWWGG	5 cut(s)	81, 243, 854, 1061, 1564
TaaI	ACNGT	7 cut(s)	22, 275, 305, 389, 447, 1229, 1237
TaiI	ACGT	1 cut(s)	1497
TaqI	TCGA	7 cut(s)	396, 732, 850, 962, 1113, 1200, 1356
TasI	AATT	6 cut(s)	161, 469, 680, 758, 1298, 1319
TauI	GCSGC	7 cut(s)	38, 74, 77, 80, 594, 1027, 1030
TfiI	GAWTC	5 cut(s)	319, 393, 506, 1208, 1475
TscAI	CASTG	1 cut(s)	1234
TseFI	GTSAC	1 cut(s)	1253
TseI	GCWGC	2 cut(s)	845, 997
Tsp45I	GTSAC	1 cut(s)	1253
TspDTI	ATGAA	2 cut(s)	674, 1427
TspGWI	ACGGA	2 cut(s)	1229, 1518
TspMI	CCCGGG	1 cut(s)	992
TspRI	CASTG	1 cut(s)	1234
Tth111I	GACNNNGTC	1 cut(s)	1450
Van91I	CCANNNNNTGG	2 cut(s)	12, 1370
VpaK11BI	GGWCC	1 cut(s)	692
XapI	RAATTY	3 cut(s)	161, 680, 1319
XbaI	TCTAGA	1 cut(s)	1478
XceI	RCATGY	1 cut(s)	1004
XcmI	CCANNNNNNNNNTGG	1 cut(s)	763
XhoI	CTCGAG	2 cut(s)	1112, 1199
XmaI	CCCGGG	1 cut(s)	992
XmaJI	CCTAGG	1 cut(s)	854
XmiI	GTMKAC	2 cut(s)	732, 1267
XmnI	GAANNNNTTC	1 cut(s)	836
XspI	CTAG	5 cut(s)	423, 606, 657, 855, 1479

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.