Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr13

Orthogroup ID

OG0014248

Physical Location & Seq

Genomic Coordinates Forward (+)

5727763 .. 5730551

Gene Structure 2789 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom13g08630.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1623 bp

ATGGCCAAACCCTGGGGAGGTGTCGGCGCCTGGGCCGCCGAGACTGAGCGAGCCGACGCTGAGCGAGCCGAGGCTGAGGAGCTCGCGGCTCAGAGCTTCCCCAGCCTAAAGGAAGCCTCCAGCGCCAAGTCGAAGAAGAAGAAGATGAGCCTCTCCGAATTCTGCAACTCTTCCGGCACCGCGGCCTCTAATCGGGTCGGTCTCACTCCCGACGAGATGATGCGCCTCCCTACCGGGCCCAAGGAGCGCTCTGCCGAAGATATGCAGTACGGTCGCCTAGGTGGCGGGTTTTCCTCTTATGGTCGCTCAGGTCCGAATCCGGGTCGGGACGGGGACAGAGAGGGCGGAGACGGGTCATGGGGTGGCGGTGGAAGAAGATCGATCTACGGTGGATTCGACGACGACCGGAGGGGCCCGCCTTCTAGGGTTTCTGATTTCGATCAGCCGTCAAGGGCGGACGAGGTTGACAATTGGGCAATGACTAAAAAATCTCTTCCTTCTGCGGATTCGAGTAGGCAGAGCCGGTATAGCTCGCTCGGTAGTGGTGGTGCTGGGGGTGGTGGTGATGGTGGTGGTATGGGCGCCCCAGCTTGGTCTAGGGCAGACGAGGTTGATAACTGGACTGTTGGGAAGAAGCCTGCTCCTTCTAGGTCATCCACATTTGGGTCTGGATTTCGCGATTCGGGTGCAGAGCCGGATCGCTGGGCTAGAGGCGGACGTGAGGTCGACAGAGTTGAGAGAGAGCGACCCAAATTGGTGTTGGATCCTCCGAAGGGCGAGAATGAGCCATTGCAGGTTGTGAAGACCAATAAGCCAAACCCATTCGGAGCAGCTCGACCTAGGGAGGAGATTTTGGCTGAGAAGGGGATGGATTGGAAGAAGCTGGAGTCGGAAATTGAAGCCAAGAAGACGAGTAGGCCGACGAGTGCCCACTCAAGCAGGCCTTCGAGTGCTCAGTCAAGCCGGTCTGAAGGGCCCGGGCTGCATGGGAGTGATAATGCAGTGAAGCCGCGGCCGAAAGTAAACCCCTTTGGCGATGCCAAGCCAAGGGAGGTTTTGCTGGAGGAGCGGGGTAAGGATTGGCGGAAGATTGATCTCGAGCTGGAGCATCAGGGTATTGACAGACCTGAAACAGAGGAGGAAAAGATTTTGAAGGAAGAAATAGATCATCTAAAGAAGGAACTCGAGAAAGAATCTACGGATAAAGTGAATAGTGAAACTGTGCGAGAGTCTGGCGGTGACCAGCCAAGTCTACATGATATAGTACTTCGGAAGGAAAGGGAATTGGAGACATTGATCCGAAATTTGGATGACAAAGTTCGCTTTGGGCAGAAGGCCATCGATAGGCCTGGGTCTGGGACTGGTAGGCCTGGGTCTGGAGCAGGTAGGCCTGGGTCTGCAGCAGGCAGGGGTGGTAGCTTTTTTGAGAGGACGCCTTCTTATTCCGGGTCATTTGAAGATTCTAGAAGCATGGAATATGTTGATGGGCCTCCCTCGCAAGGCAAAGGAGATCCGTGGGCAAGGCCTTTTGATGACAGAAGGTCATTCCATGGTGGCAGGGAGAGAGGAGGATTTCTGGGGAACAGAGACATTGACAGGGCAAGGTCAAGAGAAAGATGGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0003743 GO:0005488 GO:0005515 GO:0005575 GO:0005618 GO:0005623 GO:0005911 GO:0006412 GO:0006413 GO:0006518 GO:0006807 GO:0008135 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009505 GO:0009506 GO:0009987 GO:0010467 GO:0019538 GO:0030054 GO:0030312 GO:0034641 GO:0034645 GO:0042802 GO:0042803 GO:0043043 GO:0043170 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044464 GO:0046983 GO:0055044 GO:0071704 GO:0071944 GO:0097159 GO:1901363 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

eIF-4B

Protein Analysis

541

Amino Acids

58.78

Weight (kDa)

7.74

Isoelectric Point (pI)

49.55

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
eIF-4B	PF06273	1 - 459	1e-209	Plant specific eukaryotic initiation factor 4B
eIF-4B	PF06273	462 - 523	1.1e-17	Plant specific eukaryotic initiation factor 4B

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0014248)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G13020
fragaria_vesca	FvH4_4g26000
malus_domestica	MD13G1098600.v1.1 MD16G1099600.v1.1
prunus_persica	Prupe.1G246800_v2.0.a1 Prupe.1G246800_v2.0.a1
pyrus_communis	pycom13g08630 pycom16g08470
rosa_chinensis	RchiOBHm_Chr4g0433421
rosa_laevigata	RLG00000006754
rosa_roxburghii	Rroxscaffold_5G00374970
rosa_rugosa	Rorug04G0268700
rosa_samantha	Rh4AG323400 Rh4BG331300 Rh4CG346600 Rh4DG327600
rosa_wichuraiana	Rw4G028020

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	2 cut(s)	784, 1373
AccB1I	GGYRCC	3 cut(s)	26, 176, 581
AccB7I	CCANNNNNTGG	1 cut(s)	12
AccBSI	CCGCTC	1 cut(s)	1069
AccI	GTMKAC	2 cut(s)	726, 1252
AccII	CGCG	4 cut(s)	86, 182, 678, 1012
AclWI	GGATC	5 cut(s)	705, 758, 771, 1291, 1505
AcoI	YGGCCR	2 cut(s)	3, 1013
AcsI	RAATTY	2 cut(s)	158, 1303
AcuI	CTGAAG	1 cut(s)	990
AcyI	GRCGYC	3 cut(s)	27, 582, 1433
AfaI	GTAC	2 cut(s)	269, 1266
AfeI	AGCGCT	1 cut(s)	248
AgsI	TTSAA	3 cut(s)	899, 1153, 1457
AhdI	GACNNNNNGTC	2 cut(s)	722, 1540
AjiI	CACGTC	1 cut(s)	719
AjnI	CCWGG	5 cut(s)	11, 29, 1348, 1369, 1390
AluBI	AGCT	8 cut(s)	82, 96, 531, 590, 833, 883, 1102, 1419
AluI	AGCT	8 cut(s)	82, 96, 531, 590, 833, 883, 1102, 1419
Alw21I	GWGCWC	2 cut(s)	84, 955
Alw26I	GTCTC	5 cut(s)	35, 206, 342, 1283, 1581
AlwI	GGATC	5 cut(s)	705, 758, 771, 1291, 1505
Ama87I	CYCGRG	3 cut(s)	977, 1097, 1184
Aor51HI	AGCGCT	1 cut(s)	248
ApaI	GGGCCC	3 cut(s)	240, 416, 978
ApeKI	GCWGC	3 cut(s)	830, 982, 1400
ApoI	RAATTY	2 cut(s)	158, 1303
AspA2I	CCTAGG	2 cut(s)	277, 839
AspLEI	GCGC	5 cut(s)	29, 125, 225, 249, 584
AspS9I	GGNCC	9 cut(s)	33, 236, 237, 311, 412, 413, 974, 975, 1487
AsuC2I	CCSGG	5 cut(s)	235, 321, 978, 979, 1447
AsuHPI	GGTGA	2 cut(s)	575, 1250
AvaI	CYCGRG	3 cut(s)	977, 1097, 1184
AvaII	GGWCC	1 cut(s)	311
AvrII	CCTAGG	2 cut(s)	277, 839
BaeGI	GKGCMC	4 cut(s)	240, 416, 931, 978
BalI	TGGCCA	1 cut(s)	5
BamHI	GGATCC	1 cut(s)	763
BanI	GGYRCC	3 cut(s)	26, 176, 581
BanII	GRGCYC	4 cut(s)	84, 240, 416, 978
BbsI	GAAGAC	2 cut(s)	809, 914
Bbv12I	GWGCWC	2 cut(s)	84, 955
BbvCI	CCTCAGC	1 cut(s)	75
BbvI	GCAGC	3 cut(s)	842, 969, 1412
BccI	CCATC	5 cut(s)	560, 862, 1346, 1478, 1611
BceAI	ACGGC	1 cut(s)	430
BcgI	CGANNNNNNTGC	4 cut(s)	254, 288, 668, 702
BciT130I	CCWGG	5 cut(s)	13, 31, 1350, 1371, 1392
BcnI	CCSGG	5 cut(s)	235, 321, 978, 979, 1447
BcoDI	GTCTC	5 cut(s)	35, 206, 342, 1283, 1581
BfaI	CTAG	7 cut(s)	278, 423, 597, 648, 708, 840, 1464
BfmI	CTRYAG	1 cut(s)	1398
BfoI	RGCGCY	4 cut(s)	30, 126, 250, 585
BfuAI	ACCTGC	2 cut(s)	784, 1373
BglI	GCCNNNNNGGC	1 cut(s)	282
BisI	GCNGC	8 cut(s)	36, 87, 183, 831, 983, 1010, 1013, 1401
BlnI	CCTAGG	2 cut(s)	277, 839
BlpI	GCTNAGC	1 cut(s)	60
BlsI	GCNGC	8 cut(s)	37, 88, 184, 832, 984, 1011, 1014, 1402
BmcAI	AGTACT	1 cut(s)	1266
Bme18I	GGWCC	1 cut(s)	311
BmeRI	GACNNNNNGTC	2 cut(s)	722, 1540
BmeT110I	CYCGRG	3 cut(s)	977, 1097, 1184
BmgBI	CACGTC	1 cut(s)	719
BmgT120I	GGNCC	9 cut(s)	33, 236, 237, 311, 412, 413, 974, 975, 1487
BmiI	GGNNCC	8 cut(s)	28, 178, 238, 413, 414, 583, 765, 976
BmsI	GCATC	3 cut(s)	210, 1027, 1117
BpiI	GAAGAC	2 cut(s)	809, 914
BpmI	CTGGAG	5 cut(s)	103, 905, 1082, 1124, 1398
Bpu10I	CCTNAGC	2 cut(s)	75, 307
Bpu1102I	GCTNAGC	1 cut(s)	60
BpuEI	CTTGAG	1 cut(s)	919
BpuMI	CCSGG	5 cut(s)	235, 321, 978, 979, 1447
Bsa29I	ATCGAT	2 cut(s)	380, 1341
BsaBI	GATNNNNATC	1 cut(s)	438
BsaHI	GRCGYC	3 cut(s)	27, 582, 1433
BsaI	GGTCTC	1 cut(s)	206
BsaWI	WCCGGW	1 cut(s)	405
Bse118I	RCCGGY	2 cut(s)	522, 963
Bse1I	ACTGG	2 cut(s)	623, 1366
Bse3DI	GCAATG	2 cut(s)	483, 788
Bse8I	GATNNNNATC	1 cut(s)	438
BseBI	CCWGG	5 cut(s)	13, 31, 1350, 1371, 1392
BseCI	ATCGAT	2 cut(s)	380, 1341
BseGI	GGATG	3 cut(s)	653, 873, 1315
BseJI	GATNNNNATC	1 cut(s)	438
BseMI	GCAATG	2 cut(s)	483, 788
BseMII	CTCAG	7 cut(s)	36, 51, 66, 104, 321, 849, 968
BseNI	ACTGG	2 cut(s)	623, 1366
BseRI	GAGGAG	5 cut(s)	92, 860, 1079, 1151, 1581
BseSI	GKGCMC	4 cut(s)	240, 416, 931, 978
BseX3I	CGGCCG	1 cut(s)	1013
BseXI	GCAGC	3 cut(s)	842, 969, 1412
BseYI	CCCAGC	4 cut(s)	101, 551, 586, 702
BsgI	GTGCAG	1 cut(s)	708
Bsh1236I	CGCG	4 cut(s)	86, 182, 678, 1012
Bsh1285I	CGRYCG	3 cut(s)	274, 406, 1016
BshNI	GGYRCC	3 cut(s)	26, 176, 581
BshVI	ATCGAT	2 cut(s)	380, 1341
BsiEI	CGRYCG	3 cut(s)	274, 406, 1016
BsiHKAI	GWGCWC	2 cut(s)	84, 955
BsiHKCI	CYCGRG	3 cut(s)	977, 1097, 1184
BsiSI	CCGG	9 cut(s)	174, 234, 320, 406, 523, 695, 964, 978, 1446
BslFI	GGGAC	3 cut(s)	341, 347, 1372
BsmAI	GTCTC	5 cut(s)	35, 206, 342, 1283, 1581
BsmBI	CGTCTC	1 cut(s)	342
BsmFI	GGGAC	3 cut(s)	341, 347, 1372
Bso31I	GGTCTC	1 cut(s)	206
BsoBI	CYCGRG	3 cut(s)	977, 1097, 1184
Bsp120I	GGGCCC	3 cut(s)	236, 412, 974
Bsp1286I	GDGCHC	6 cut(s)	84, 240, 416, 931, 955, 978
Bsp143I	GATC	9 cut(s)	377, 381, 439, 697, 763, 1093, 1165, 1296, 1510
Bsp1720I	GCTNAGC	1 cut(s)	60
Bsp19I	CCATGG	1 cut(s)	1549
Bsp68I	TCGCGA	1 cut(s)	678
BspCNI	CTCAG	7 cut(s)	37, 52, 67, 103, 320, 850, 967
BspDI	ATCGAT	2 cut(s)	380, 1341
BspFNI	CGCG	4 cut(s)	86, 182, 678, 1012
BspLI	GGNNCC	8 cut(s)	28, 178, 238, 413, 414, 583, 765, 976
BspMAI	CTGCAG	1 cut(s)	1402
BspMI	ACCTGC	2 cut(s)	784, 1373
BspPI	GGATC	5 cut(s)	705, 758, 771, 1291, 1505
BspT107I	GGYRCC	3 cut(s)	26, 176, 581
BspTNI	GGTCTC	1 cut(s)	206
BsrBI	CCGCTC	1 cut(s)	1069
BsrDI	GCAATG	2 cut(s)	483, 788
BsrFI	RCCGGY	2 cut(s)	522, 963
BsrI	ACTGG	2 cut(s)	623, 1366
BssAI	RCCGGY	2 cut(s)	522, 963
BssMI	GATC	9 cut(s)	377, 381, 439, 697, 763, 1093, 1165, 1296, 1510
BssNI	GRCGYC	3 cut(s)	27, 582, 1433
BssT1I	CCWWGG	5 cut(s)	240, 277, 839, 1046, 1549
Bst2UI	CCWGG	5 cut(s)	13, 31, 1350, 1371, 1392
Bst4CI	ACNGT	4 cut(s)	272, 389, 625, 1222
Bst6I	CTCTTC	2 cut(s)	175, 498
BstACI	GRCGYC	3 cut(s)	27, 582, 1433
BstC8I	GCNNGC	8 cut(s)	51, 66, 84, 416, 533, 639, 941, 1405
BstDEI	CTNAG	7 cut(s)	45, 60, 75, 90, 307, 858, 954
BstDSI	CCRYGG	4 cut(s)	180, 1010, 1514, 1549
BstEII	GGTNACC	1 cut(s)	1238
BstF5I	GGATG	3 cut(s)	653, 873, 1315
BstFNI	CGCG	4 cut(s)	86, 182, 678, 1012
BstH2I	RGCGCY	4 cut(s)	30, 126, 250, 585
BstHHI	GCGC	5 cut(s)	29, 125, 225, 249, 584
BstKTI	GATC	9 cut(s)	380, 384, 442, 700, 766, 1096, 1168, 1299, 1513
BstMAI	GTCTC	5 cut(s)	35, 206, 342, 1283, 1581
BstMBI	GATC	9 cut(s)	377, 381, 439, 697, 763, 1093, 1165, 1296, 1510
BstMCI	CGRYCG	3 cut(s)	274, 406, 1016
BstNI	CCWGG	5 cut(s)	13, 31, 1350, 1371, 1392
BstPI	GGTNACC	1 cut(s)	1238
BstSFI	CTRYAG	1 cut(s)	1398
BstSLI	GKGCMC	4 cut(s)	240, 416, 931, 978
BstUI	CGCG	4 cut(s)	86, 182, 678, 1012
BstV1I	GCAGC	3 cut(s)	842, 969, 1412
BstV2I	GAAGAC	2 cut(s)	809, 914
BstX2I	RGATCY	2 cut(s)	763, 1510
BstYI	RGATCY	2 cut(s)	763, 1510
BstZI	CGGCCG	1 cut(s)	1013
Bsu15I	ATCGAT	2 cut(s)	380, 1341
BsuTUI	ATCGAT	2 cut(s)	380, 1341
BtgI	CCRYGG	4 cut(s)	180, 1010, 1514, 1549
BtgZI	GCGATG	1 cut(s)	1050
BtrI	CACGTC	1 cut(s)	719
BtsCI	GGATG	3 cut(s)	653, 873, 1315
BtsI	GCAGTG	1 cut(s)	1008
BtsIMutI	CAGTG	1 cut(s)	1008
BtuMI	TCGCGA	1 cut(s)	678
BveI	ACCTGC	2 cut(s)	784, 1373
Cac8I	GCNNGC	8 cut(s)	51, 66, 84, 416, 533, 639, 941, 1405
CfoI	GCGC	5 cut(s)	29, 125, 225, 249, 584
Cfr10I	RCCGGY	2 cut(s)	522, 963
Cfr13I	GGNCC	9 cut(s)	33, 236, 237, 311, 412, 413, 974, 975, 1487
Cfr42I	CCGCGG	2 cut(s)	183, 1013
Cfr9I	CCCGGG	1 cut(s)	977
ClaI	ATCGAT	2 cut(s)	380, 1341
CseI	GACGC	2 cut(s)	65, 1441
Csp6I	GTAC	2 cut(s)	268, 1265
CviAII	CATG	5 cut(s)	357, 986, 1256, 1471, 1550
CviQI	GTAC	2 cut(s)	268, 1265
DdeI	CTNAG	7 cut(s)	45, 60, 75, 90, 307, 858, 954
DinI	GGCGCC	2 cut(s)	28, 583
DpnI	GATC	9 cut(s)	379, 383, 441, 699, 765, 1095, 1167, 1298, 1512
DpnII	GATC	9 cut(s)	377, 381, 439, 697, 763, 1093, 1165, 1296, 1510
DriI	GACNNNNNGTC	2 cut(s)	722, 1540
EaeI	YGGCCR	2 cut(s)	3, 1013
EagI	CGGCCG	1 cut(s)	1013
Eam1104I	CTCTTC	2 cut(s)	175, 498
Eam1105I	GACNNNNNGTC	2 cut(s)	722, 1540
EarI	CTCTTC	2 cut(s)	175, 498
EciI	GGCGGA	4 cut(s)	360, 470, 729, 1099
Ecl136II	GAGCTC	1 cut(s)	82
EclXI	CGGCCG	1 cut(s)	1013
Eco130I	CCWWGG	5 cut(s)	240, 277, 839, 1046, 1549
Eco147I	AGGCCT	5 cut(s)	943, 1348, 1369, 1390, 1525
Eco24I	GRGCYC	4 cut(s)	84, 240, 416, 978
Eco31I	GGTCTC	1 cut(s)	206
Eco47I	GGWCC	1 cut(s)	311
Eco47III	AGCGCT	1 cut(s)	248
Eco52I	CGGCCG	1 cut(s)	1013
Eco53kI	GAGCTC	1 cut(s)	82
Eco57I	CTGAAG	1 cut(s)	990
Eco88I	CYCGRG	3 cut(s)	977, 1097, 1184
Eco91I	GGTNACC	1 cut(s)	1238
EcoICRI	GAGCTC	1 cut(s)	82
EcoO109I	RGGNCCY	2 cut(s)	412, 974
EcoO65I	GGTNACC	1 cut(s)	1238
EcoRI	GAATTC	1 cut(s)	158
EcoRII	CCWGG	5 cut(s)	11, 29, 1348, 1369, 1390
EcoT14I	CCWWGG	5 cut(s)	240, 277, 839, 1046, 1549
EcoT38I	GRGCYC	4 cut(s)	84, 240, 416, 978
EgeI	GGCGCC	2 cut(s)	28, 583
EheI	GGCGCC	2 cut(s)	28, 583
ErhI	CCWWGG	5 cut(s)	240, 277, 839, 1046, 1549
Esp3I	CGTCTC	1 cut(s)	342
FaeI	CATG	5 cut(s)	360, 989, 1259, 1474, 1553
FalI	AAGNNNNNCTT	2 cut(s)	928, 960
FaqI	GGGAC	3 cut(s)	341, 347, 1372
FatI	CATG	5 cut(s)	356, 985, 1255, 1470, 1549
FauI	CCCGC	3 cut(s)	278, 423, 1062
FblI	GTMKAC	2 cut(s)	726, 1252
Fnu4HI	GCNGC	8 cut(s)	36, 87, 183, 831, 983, 1010, 1013, 1401
FokI	GGATG	3 cut(s)	640, 880, 1322
FriOI	GRGCYC	4 cut(s)	84, 240, 416, 978
Fsp4HI	GCNGC	8 cut(s)	36, 87, 183, 831, 983, 1010, 1013, 1401
FspBI	CTAG	7 cut(s)	278, 423, 597, 648, 708, 840, 1464
GlaI	GCGC	5 cut(s)	28, 124, 224, 248, 583
GluI	GCNGC	8 cut(s)	36, 87, 183, 831, 983, 1010, 1013, 1401
GsaI	CCCAGC	4 cut(s)	105, 555, 590, 706
GsuI	CTGGAG	5 cut(s)	103, 905, 1082, 1124, 1398
HaeII	RGCGCY	4 cut(s)	30, 126, 250, 585
HapII	CCGG	9 cut(s)	174, 234, 320, 406, 523, 695, 964, 978, 1446
HgaI	GACGC	2 cut(s)	65, 1441
HhaI	GCGC	5 cut(s)	29, 125, 225, 249, 584
Hin1I	GRCGYC	3 cut(s)	27, 582, 1433
Hin1II	CATG	5 cut(s)	360, 989, 1259, 1474, 1553
Hin6I	GCGC	5 cut(s)	27, 123, 223, 247, 582
HinP1I	GCGC	5 cut(s)	27, 123, 223, 247, 582
HincII	GTYRAC	2 cut(s)	466, 727
HindII	GTYRAC	2 cut(s)	466, 727
HinfI	GANTC	8 cut(s)	316, 393, 506, 680, 887, 1193, 1229, 1460
HpaII	CCGG	9 cut(s)	174, 234, 320, 406, 523, 695, 964, 978, 1446
HphI	GGTGA	2 cut(s)	575, 1250
Hpy166II	GTNNAC	4 cut(s)	466, 727, 1024, 1253
Hpy188III	TCNNGA	9 cut(s)	209, 326, 669, 677, 1097, 1186, 1377, 1464, 1608
Hpy8I	GTNNAC	4 cut(s)	466, 727, 1024, 1253
Hpy99I	CGWCG	5 cut(s)	59, 215, 401, 404, 925
HpyCH4III	ACNGT	4 cut(s)	272, 389, 625, 1222
HpyCH4IV	ACGT	1 cut(s)	718
HpyCH4V	TGCA	7 cut(s)	165, 265, 689, 793, 985, 1001, 1400
HpyF3I	CTNAG	7 cut(s)	45, 60, 75, 90, 307, 858, 954
HpySE526I	ACGT	1 cut(s)	718
Hsp92I	GRCGYC	3 cut(s)	27, 582, 1433
Hsp92II	CATG	5 cut(s)	360, 989, 1259, 1474, 1553
HspAI	GCGC	5 cut(s)	27, 123, 223, 247, 582
KasI	GGCGCC	2 cut(s)	26, 581
KspI	CCGCGG	2 cut(s)	183, 1013
Kzo9I	GATC	9 cut(s)	377, 381, 439, 697, 763, 1093, 1165, 1296, 1510
LmnI	GCTCC	7 cut(s)	79, 244, 646, 827, 1066, 1105, 1379
Lsp1109I	GCAGC	3 cut(s)	842, 969, 1412
LweI	GCATC	3 cut(s)	210, 1027, 1117
MaeI	CTAG	7 cut(s)	278, 423, 597, 648, 708, 840, 1464
MaeII	ACGT	1 cut(s)	718
MaeIII	GTNAC	1 cut(s)	1238
MalI	GATC	9 cut(s)	379, 383, 441, 699, 765, 1095, 1167, 1298, 1512
MbiI	CCGCTC	1 cut(s)	1069
MboI	GATC	9 cut(s)	377, 381, 439, 697, 763, 1093, 1165, 1296, 1510
MfeI	CAATTG	1 cut(s)	469
MflI	RGATCY	2 cut(s)	763, 1510
MhlI	GDGCHC	6 cut(s)	84, 240, 416, 931, 955, 978
MlsI	TGGCCA	1 cut(s)	5
MluCI	AATT	6 cut(s)	158, 469, 752, 894, 1283, 1303
MluNI	TGGCCA	1 cut(s)	5
Mly113I	GGCGCC	2 cut(s)	27, 582
MlyI	GAGTC	2 cut(s)	896, 1238
MmeI	TCCRAC	2 cut(s)	741, 870
Mox20I	TGGCCA	1 cut(s)	5
MscI	TGGCCA	1 cut(s)	5
MslI	CAYNNNNRTG	1 cut(s)	990
Msp20I	TGGCCA	1 cut(s)	5
MspA1I	CMGCKG	2 cut(s)	182, 1012
MspI	CCGG	9 cut(s)	174, 234, 320, 406, 523, 695, 964, 978, 1446
MunI	CAATTG	1 cut(s)	469
MvaI	CCWGG	5 cut(s)	13, 31, 1350, 1371, 1392
MvnI	CGCG	4 cut(s)	86, 182, 678, 1012
NarI	GGCGCC	2 cut(s)	27, 582
NciI	CCSGG	5 cut(s)	235, 321, 978, 979, 1447
NcoI	CCATGG	1 cut(s)	1549
NdeII	GATC	9 cut(s)	377, 381, 439, 697, 763, 1093, 1165, 1296, 1510
NlaIII	CATG	5 cut(s)	360, 989, 1259, 1474, 1553
NlaIV	GGNNCC	8 cut(s)	28, 178, 238, 413, 414, 583, 765, 976
NmeAIII	GCCGAG	2 cut(s)	64, 94
NmuCI	GTSAC	1 cut(s)	1238
NruI	TCGCGA	1 cut(s)	678
PaeR7I	CTCGAG	2 cut(s)	1097, 1184
PasI	CCCWGGG	1 cut(s)	12
PceI	AGGCCT	5 cut(s)	943, 1348, 1369, 1390, 1525
PcsI	WCGNNNNNNNCGW	2 cut(s)	393, 917
PfeI	GAWTC	6 cut(s)	316, 393, 506, 680, 1193, 1460
PflMI	CCANNNNNTGG	1 cut(s)	12
PkrI	GCNGC	8 cut(s)	37, 88, 184, 832, 984, 1011, 1014, 1402
PleI	GAGTC	2 cut(s)	895, 1237
PluTI	GGCGCC	2 cut(s)	30, 585
PpsI	GAGTC	2 cut(s)	895, 1237
Psp124BI	GAGCTC	1 cut(s)	84
Psp6I	CCWGG	5 cut(s)	11, 29, 1348, 1369, 1390
PspEI	GGTNACC	1 cut(s)	1238
PspFI	CCCAGC	4 cut(s)	101, 551, 586, 702
PspGI	CCWGG	5 cut(s)	11, 29, 1348, 1369, 1390
PspN4I	GGNNCC	8 cut(s)	28, 178, 238, 413, 414, 583, 765, 976
PspOMI	GGGCCC	3 cut(s)	236, 412, 974
PspPI	GGNCC	9 cut(s)	33, 236, 237, 311, 412, 413, 974, 975, 1487
PstI	CTGCAG	1 cut(s)	1402
PsuI	RGATCY	2 cut(s)	763, 1510
RruI	TCGCGA	1 cut(s)	678
RsaI	GTAC	2 cut(s)	269, 1266
RsaNI	GTAC	2 cut(s)	268, 1265
RseI	CAYNNNNRTG	1 cut(s)	990
SacI	GAGCTC	1 cut(s)	84
SacII	CCGCGG	2 cut(s)	183, 1013
SalI	GTCGAC	1 cut(s)	725
SatI	GCNGC	8 cut(s)	36, 87, 183, 831, 983, 1010, 1013, 1401
Sau3AI	GATC	9 cut(s)	377, 381, 439, 697, 763, 1093, 1165, 1296, 1510
Sau96I	GGNCC	9 cut(s)	33, 236, 237, 311, 412, 413, 974, 975, 1487
ScaI	AGTACT	1 cut(s)	1266
SchI	GAGTC	2 cut(s)	896, 1238
SduI	GDGCHC	6 cut(s)	84, 240, 416, 931, 955, 978
SfaNI	GCATC	3 cut(s)	210, 1027, 1117
SfcI	CTRYAG	1 cut(s)	1398
SfoI	GGCGCC	2 cut(s)	28, 583
Sfr274I	CTCGAG	2 cut(s)	1097, 1184
Sfr303I	CCGCGG	2 cut(s)	183, 1013
SgrBI	CCGCGG	2 cut(s)	183, 1013
SinI	GGWCC	1 cut(s)	311
SlaI	CTCGAG	2 cut(s)	1097, 1184
SmaI	CCCGGG	1 cut(s)	979
SmiMI	CAYNNNNRTG	1 cut(s)	990
SmlI	CTYRAG	3 cut(s)	934, 1097, 1184
SmoI	CTYRAG	3 cut(s)	934, 1097, 1184
SrfI	GCCCGGGC	1 cut(s)	979
Sse9I	AATT	6 cut(s)	158, 469, 752, 894, 1283, 1303
SseBI	AGGCCT	5 cut(s)	943, 1348, 1369, 1390, 1525
SspDI	GGCGCC	2 cut(s)	26, 581
SspMI	CTAG	7 cut(s)	278, 423, 597, 648, 708, 840, 1464
SstI	GAGCTC	1 cut(s)	84
StuI	AGGCCT	5 cut(s)	943, 1348, 1369, 1390, 1525
StyI	CCWWGG	5 cut(s)	240, 277, 839, 1046, 1549
TaaI	ACNGT	4 cut(s)	272, 389, 625, 1222
TaiI	ACGT	1 cut(s)	721
TaqII	GACCGA	1 cut(s)	188
TasI	AATT	6 cut(s)	158, 469, 752, 894, 1283, 1303
TatI	WGTACW	1 cut(s)	1264
TauI	GCSGC	5 cut(s)	38, 89, 185, 1012, 1015
TfiI	GAWTC	6 cut(s)	316, 393, 506, 680, 1193, 1460
TscAI	CASTG	1 cut(s)	1008
TseFI	GTSAC	1 cut(s)	1238
TseI	GCWGC	3 cut(s)	830, 982, 1400
Tsp45I	GTSAC	1 cut(s)	1238
TspGWI	ACGGA	2 cut(s)	1214, 1503
TspMI	CCCGGG	1 cut(s)	977
TspRI	CASTG	1 cut(s)	1008
Van91I	CCANNNNNTGG	1 cut(s)	12
VpaK11BI	GGWCC	1 cut(s)	311
XapI	RAATTY	2 cut(s)	158, 1303
XbaI	TCTAGA	1 cut(s)	1463
XcmI	CCANNNNNNNNNTGG	1 cut(s)	757
XhoI	CTCGAG	2 cut(s)	1097, 1184
XmaI	CCCGGG	1 cut(s)	977
XmaJI	CCTAGG	2 cut(s)	277, 839
XmiI	GTMKAC	2 cut(s)	726, 1252
XspI	CTAG	7 cut(s)	278, 423, 597, 648, 708, 840, 1464
ZrmI	AGTACT	1 cut(s)	1266

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.