Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr13

Orthogroup ID

OG0014721

Physical Location & Seq

Genomic Coordinates Reverse (-)

7357722 .. 7360289

Gene Structure 2568 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD13G1103000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2568 bp

ATGTTAGGGGAAGAAGAGAAAAACCGGCTCGCCGGAAGTACTTCCCGGAAAACCTACTTGCGGTCGGTTTCGTGGTCTGACCGGTCACCCTCTAAGCATAACAATCCATATCCGAGGCCACAGCTGGTTAGTAAAGCGAGGTCCTGCCTCCCTCCACTTCAGCCCCTTTCAATAGCCAGAAATACCGTTAAGGAGTGGCCGCGGGCGGGGTCTGATGATCTCGGGGTCTGGCCTCAACCGCAGACCCCGAGAGGTTCGGCTAAACCGCTTCTGAATTCGAACCCAGAAGAACCTGGGAGAGAGTTTGAGTTTAAGAAGGATAAACTTGCGTTTTTCAACAAAGAATGCTCGAGAATTGCTGATCATATATTCTTAGGAAGTGATGCCGTGGCTAAGAACCGCGAGATTTTGAGGCAGAATGGGATCACTCATGTGCTGAACTGTGTTGGGTTTGTTTCTCCCGAGTATTTCAGAAATGATCTTGTGTACAAGACACTTTGGTTGCGAGATAGCCCATCGGAGGACATTACGAGTATACTCTATGATGTGTTTGATTATTTTGAAGATGTTCGAAAGCAAGGCGGGCGAGTTTTCGTGCATTGTTGTCAGGGAGTGTCTCGGTCCACCTCTCTGGTCATTGCATACCTAATGTGGAGGGAGGGCCACAGCTTTGAAGATGCGTTCCAGTATGTGAAGGCAGCGAGAGGGGTGACTAACCCGAATATGGGTTTTGCTTGTCAACTCCTTCAGTGCCAGAAGAGGGTACATGCTGTGCCTGCAAGCCCAAATTCCATGTTAAGGATGTATCGGATGGCCCCGCATTCATCGTATGATCCTCTTCATCTGGTGCCAAAGACGTTGGGACATCCAGGTCCACAAGGACTCGACTCTCGTGGGGCGTTCATTGTGCATGTTCCATCTGCTATATACGTCTGGATTGGAAAGGATTGCAACACAATGATGTCAGATAATGCAAAGGCAGCTGCCTTTCAGGTCATCCAGTATGAGAGGGCAAAGGGCCCGATTGTGGACATCAATGAAGGTGAAGAACCGTTGGAGTTCTGGGATGCTCTTTCCAACGGACTCTTAGCAGAAGATTCCAGCAAGACAGATGCCAAAAAGGTAGAAACTTTTTCTTCCGCAGGTGATAAGGTTTCTGCAGCGATGTGTGGTCAGGTTGGTGAAAGGAAGGTTGCCGAATATAATTTGGATTTCGAAATTTTTCATAGGGTACTTGCAGGTGGGGTTGTTCCACCTTTTTCAGTATCAAATACCGAATCAGAAACTTGCCTTCCAGCCAGAGAAAATGGATGGGGTAGATTGCGGCAAAAGTTTGCAAGCGGAATTATGAAAGGATTGGTCACATCTTCTGAGGTGAACCGAAGCCCTACCCCGTCCGGTGATGAATCGGATATGGTTGTGGAAACTCATAAAGAAGCAGAAGACTCCGTTTCCCCAATCGAGCCTTCGTCGCCATTATCAGCATCACGTCACTTTTGTCGTTCACCAGATTCCTTTGAATGTTATCCAAATAAAAGCCCACGTAGGGTAACAGATACTGTTAGAGAGGTAGAACGCTTTGCTCCTCTGACCGATAAGTTACTGTTACCTACAACTCTTTGTGGTTCACCTGATTCATTTTCTTGTTTTCCTGACAGAAGTCCCAAGTTCAGCTCCAAATCCCCAACACTCTCCCCTTCAACCTCTGAAAACTCCAGTTCATTTACCTTTTCACCCTCATCTTCCAATTGGTCGGACTTGTCATATTTGTCTTCTCGACAGCCTTCACCTTCTGGCTTGGATTCCACAGATCCATTTAATGTTAAGAATATCTCTTTGGCAGATAACTCATGCTTGCTTTTCAAAAAATCTCCCCCGTCGCCTACAGAAGCATTTTCTCCTGATTCTACTCTGGAAATGGCAAATACAGGTTTTCCATGTAAGGGGATTTCCCCCTCTATTGCGGAGCGTAGAGGGAGTAATCCTCCACCTCGAATGTTGGTACCTTTGGTTGATGAAACACCAAAAGTTCCGAGGAATCTGGTACGCTCACGGTCCTTCTCCTTACCTGACATGGCTGATGATGCAATGGACACTGATTGCAACCAATCTGAAACTGTGAGCAACAGAGAAGACCTAATGTTAGATGTGAACGTTAGTCACCATGGAAACGAATTGAAATCTGAAAGGATCACGCAAGCAGCGAAAGTGATCAACCCTGTTTTGTACCAGTGGCCTTCTTTGAATAAAGTGGAGGCACATCAGTCTCACGAACTTCATACTGGATCAGCGTATCTTTTGCCAGCTCCAGATACGAGTGCAGGCACAAGTAATCCTGGTATCTTATTTGTCTGGTTGGGACTCGAAGTATTGCAGGAGGGAGGGCAAAGTCCGACATGTGAGGATAGACATCTTCACTGGGAGGCCATAGGACACAACTTGCTCGATCGCATGGGTTTGCCTATGAATTCCCCTGTACAGGTAATTACCATACATTGTTTCTCTGTTTTTCATTATTGCTTAATTGCTTCTGTAATTTCTAACCAATGTCTGATTAATGCACATTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000188 GO:0001704 GO:0001706 GO:0001932 GO:0001933 GO:0003674 GO:0003824 GO:0004721 GO:0004722 GO:0004725 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006464 GO:0006469 GO:0006470 GO:0006793 GO:0006796 GO:0006807 GO:0007275 GO:0007369 GO:0007492 GO:0008150 GO:0008152 GO:0008330 GO:0009653 GO:0009790 GO:0009888 GO:0009892 GO:0009966 GO:0009968 GO:0009987 GO:0010563 GO:0010605 GO:0010646 GO:0010648 GO:0016311 GO:0016787 GO:0016788 GO:0016791 GO:0019220 GO:0019222 GO:0019538 GO:0023051 GO:0023057 GO:0031323 GO:0031324 GO:0031399 GO:0031400 GO:0032268 GO:0032269 GO:0032501 GO:0032502 GO:0033549 GO:0033673 GO:0035335 GO:0035970 GO:0036211 GO:0042325 GO:0042326 GO:0042578 GO:0043086 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043405 GO:0043407 GO:0043408 GO:0043409 GO:0043412 GO:0043549 GO:0044092 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0045859 GO:0045936 GO:0048519 GO:0048523 GO:0048583 GO:0048585 GO:0048598 GO:0048646 GO:0048856 GO:0050789 GO:0050790 GO:0050794 GO:0051171 GO:0051172 GO:0051174 GO:0051246 GO:0051248 GO:0051338 GO:0051348 GO:0060255 GO:0065007 GO:0065009 GO:0070372 GO:0070373 GO:0071704 GO:0071900 GO:0071901 GO:0080090 GO:0140096 GO:1901564 GO:1902531 GO:1902532 GO:1990439

KEGG Pathways

Metabolic & Signaling

ko04010 ko04726 ko05418 map04010 map04726 map05418

Pfam Domains

Protein Families

DSPc Gelsolin

Protein Analysis

856

Amino Acids

94.58

Weight (kDa)

6.14

Isoelectric Point (pI)

57.55

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DSPc	PF00782	123 - 253	2.7e-27	Dual specificity phosphatase, catalytic domain
MPK1_gelsolin_C	PF25466	744 - 830	2.7e-19	MPK1 gelsolin domain C-terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0014721)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_4g25430 FvH4_4g25430
malus_domestica	MD13G1103000.v1.1 MD16G1103200.v1.1
prunus_persica	Prupe.1G242800_v2.0.a1
pyrus_communis	pycom16g08800
rosa_chinensis	RchiOBHm_Chr4g0432741
rosa_laevigata	RLG00000006816
rosa_multiflora	Rmu_co8494851.1_g000001 Rmu_sc0012180.1_g000009
rosa_roxburghii	Rroxscaffold_5G00374200
rosa_rugosa	Rorug04G0262100
rosa_samantha	Rh4AG316600 Rh4BG324700 Rh4CG339700 Rh4DG319900
rosa_wichuraiana	Rw4G027540

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	2 cut(s)	1133, 1229
AasI	GACNNNNNNGTC	1 cut(s)	870
Acc36I	ACCTGC	2 cut(s)	1133, 1229
Acc65I	GGTACC	1 cut(s)	2002
AccB1I	GGYRCC	2 cut(s)	847, 2002
AccI	GTMKAC	1 cut(s)	535
AccII	CGCG	2 cut(s)	202, 402
AclI	AACGTT	1 cut(s)	2154
AclWI	GGATC	5 cut(s)	431, 827, 1805, 2198, 2293
AcoI	YGGCCR	1 cut(s)	197
AcsI	RAATTY	4 cut(s)	274, 787, 1218, 2467
AcuI	CTGAAG	2 cut(s)	143, 731
AfaI	GTAC	8 cut(s)	40, 488, 765, 1233, 2004, 2046, 2228, 2478
AflIII	ACRYGT	1 cut(s)	2396
AgeI	ACCGGT	1 cut(s)	81
AgsI	TTSAA	9 cut(s)	171, 337, 563, 674, 1520, 1701, 1864, 2179, 2245
AjiI	CACGTC	1 cut(s)	1490
AjnI	CCWGG	3 cut(s)	292, 868, 2335
AjuI	GAANNNNNNNTTGG	4 cut(s)	1450, 1482, 1820, 1852
AleI	CACNNNNGTG	1 cut(s)	431
AluBI	AGCT	5 cut(s)	124, 669, 983, 1674, 2306
AluI	AGCT	5 cut(s)	124, 669, 983, 1674, 2306
Alw26I	GTCTC	2 cut(s)	621, 2271
AlwI	GGATC	5 cut(s)	431, 827, 1805, 2198, 2293
AlwNI	CAGNNNCTG	1 cut(s)	1559
Ama87I	CYCGRG	4 cut(s)	221, 247, 349, 461
AoxI	GGCC	8 cut(s)	116, 197, 230, 661, 813, 1018, 2234, 2424
ApaI	GGGCCC	1 cut(s)	1022
ApeKI	GCWGC	5 cut(s)	698, 980, 983, 1160, 2201
ApoI	RAATTY	4 cut(s)	274, 787, 1218, 2467
AseI	ATTAAT	1 cut(s)	2556
AsiGI	ACCGGT	1 cut(s)	81
Asp700I	GAANNNNTTC	3 cut(s)	40, 567, 1221
Asp718I	GGTACC	1 cut(s)	2002
AspS9I	GGNCC	8 cut(s)	141, 621, 661, 814, 872, 1018, 1019, 2055
AsuC2I	CCSGG	1 cut(s)	46
AsuII	TTCGAA	3 cut(s)	278, 571, 1215
AvaI	CYCGRG	4 cut(s)	221, 247, 349, 461
AvaII	GGWCC	4 cut(s)	141, 621, 872, 2055
BaeGI	GKGCMC	1 cut(s)	1022
BanI	GGYRCC	2 cut(s)	847, 2002
BanII	GRGCYC	1 cut(s)	1022
BauI	CACGAG	1 cut(s)	891
BbsI	GAAGAC	3 cut(s)	1449, 1764, 2139
BbvI	GCAGC	5 cut(s)	710, 970, 992, 1172, 2213
BccI	CCATC	4 cut(s)	523, 805, 925, 1305
BceAI	ACGGC	1 cut(s)	371
BciT130I	CCWGG	3 cut(s)	294, 870, 2337
BclI	TGATCA	2 cut(s)	361, 2211
BcnI	CCSGG	1 cut(s)	46
BcoDI	GTCTC	2 cut(s)	621, 2271
BfmI	CTRYAG	2 cut(s)	1158, 1884
BfuAI	ACCTGC	2 cut(s)	1133, 1229
BisI	GCNGC	7 cut(s)	200, 699, 981, 984, 1161, 1325, 2202
BlsI	GCNGC	7 cut(s)	201, 700, 982, 985, 1162, 1326, 2203
BmcAI	AGTACT	1 cut(s)	40
Bme1390I	CCNGG	4 cut(s)	46, 294, 870, 2337
Bme18I	GGWCC	4 cut(s)	141, 621, 872, 2055
BmeT110I	CYCGRG	4 cut(s)	221, 247, 349, 461
BmgBI	CACGTC	1 cut(s)	1490
BmgT120I	GGNCC	8 cut(s)	141, 621, 661, 814, 872, 1018, 1019, 2055
BmiI	GGNNCC	4 cut(s)	816, 849, 1020, 2004
BmrFI	CCNGG	4 cut(s)	46, 294, 870, 2337
BmrI	ACTGGG	1 cut(s)	2428
BmsI	GCATC	6 cut(s)	373, 667, 1057, 1102, 1493, 2074
BmuI	ACTGGG	1 cut(s)	2428
BoxI	GACNNNNGTC	1 cut(s)	1659
BpiI	GAAGAC	3 cut(s)	1449, 1764, 2139
BplI	GAGNNNNNCTC	2 cut(s)	1969, 2001
BpmI	CTGGAG	2 cut(s)	1699, 2292
Bpu14I	TTCGAA	3 cut(s)	278, 571, 1215
BpuMI	CCSGG	1 cut(s)	46
BsaAI	YACGTR	1 cut(s)	1544
BsaBI	GATNNNNATC	1 cut(s)	2409
BsaJI	CCNNGG	6 cut(s)	113, 200, 293, 387, 2033, 2164
BsaWI	WCCGGW	2 cut(s)	81, 1397
Bse118I	RCCGGY	2 cut(s)	24, 81
Bse1I	ACTGG	6 cut(s)	685, 1000, 1716, 2230, 2287, 2423
Bse3DI	GCAATG	2 cut(s)	636, 2094
Bse8I	GATNNNNATC	1 cut(s)	2409
BseBI	CCWGG	3 cut(s)	294, 870, 2337
BseDI	CCNNGG	6 cut(s)	113, 200, 293, 387, 2033, 2164
BseGI	GGATG	6 cut(s)	807, 816, 865, 996, 1072, 1316
BseJI	GATNNNNATC	1 cut(s)	2409
BseMI	GCAATG	2 cut(s)	636, 2094
BseMII	CTCAG	1 cut(s)	1362
BseNI	ACTGG	6 cut(s)	685, 1000, 1716, 2230, 2287, 2423
BseRI	GAGGAG	1 cut(s)	1575
BseSI	GKGCMC	1 cut(s)	1022
BseXI	GCAGC	5 cut(s)	710, 970, 992, 1172, 2213
BsgI	GTGCAG	1 cut(s)	2340
Bsh1236I	CGCG	2 cut(s)	202, 402
Bsh1285I	CGRYCG	2 cut(s)	65, 2449
BshFI	GGCC	8 cut(s)	118, 199, 232, 663, 815, 1020, 2236, 2426
BshNI	GGYRCC	2 cut(s)	847, 2002
BshTI	ACCGGT	1 cut(s)	81
BsiEI	CGRYCG	2 cut(s)	65, 2449
BsiHKCI	CYCGRG	4 cut(s)	221, 247, 349, 461
BsiSI	CCGG	5 cut(s)	25, 33, 46, 82, 1398
BslFI	GGGAC	3 cut(s)	876, 1647, 2373
BsmAI	GTCTC	2 cut(s)	621, 2271
BsmFI	GGGAC	3 cut(s)	876, 1647, 2373
BsmI	GAATGC	2 cut(s)	350, 820
BsnI	GGCC	8 cut(s)	118, 199, 232, 663, 815, 1020, 2236, 2426
BsoBI	CYCGRG	4 cut(s)	221, 247, 349, 461
Bsp119I	TTCGAA	3 cut(s)	278, 571, 1215
Bsp120I	GGGCCC	1 cut(s)	1018
Bsp1286I	GDGCHC	1 cut(s)	1022
Bsp1407I	TGTACA	2 cut(s)	486, 2476
Bsp19I	CCATGG	1 cut(s)	2164
BspANI	GGCC	8 cut(s)	118, 199, 232, 663, 815, 1020, 2236, 2426
BspCNI	CTCAG	1 cut(s)	1363
BspFNI	CGCG	2 cut(s)	202, 402
BspLI	GGNNCC	4 cut(s)	816, 849, 1020, 2004
BspMAI	CTGCAG	1 cut(s)	1162
BspMI	ACCTGC	2 cut(s)	1133, 1229
BspPI	GGATC	5 cut(s)	431, 827, 1805, 2198, 2293
BspT104I	TTCGAA	3 cut(s)	278, 571, 1215
BspT107I	GGYRCC	2 cut(s)	847, 2002
BsrDI	GCAATG	2 cut(s)	636, 2094
BsrFI	RCCGGY	2 cut(s)	24, 81
BsrGI	TGTACA	2 cut(s)	486, 2476
BsrI	ACTGG	6 cut(s)	685, 1000, 1716, 2230, 2287, 2423
BssAI	RCCGGY	2 cut(s)	24, 81
BssECI	CCNNGG	6 cut(s)	113, 200, 293, 387, 2033, 2164
BssNAI	GTATAC	1 cut(s)	536
BssSI	CACGAG	1 cut(s)	891
BssT1I	CCWWGG	1 cut(s)	2164
Bst1107I	GTATAC	1 cut(s)	536
Bst2BI	CACGAG	1 cut(s)	891
Bst2UI	CCWGG	3 cut(s)	294, 870, 2337
Bst4CI	ACNGT	7 cut(s)	187, 443, 1053, 1561, 1605, 2055, 2119
Bst6I	CTCTTC	3 cut(s)	9, 752, 843
BstAUI	TGTACA	2 cut(s)	486, 2476
BstBAI	YACGTR	1 cut(s)	1544
BstBI	TTCGAA	3 cut(s)	278, 571, 1215
BstDEI	CTNAG	5 cut(s)	93, 373, 393, 1087, 1371
BstDSI	CCRYGG	3 cut(s)	200, 387, 2164
BstEII	GGTNACC	1 cut(s)	84
BstF5I	GGATG	6 cut(s)	807, 816, 865, 996, 1072, 1316
BstFNI	CGCG	2 cut(s)	202, 402
BstMAI	GTCTC	2 cut(s)	621, 2271
BstMCI	CGRYCG	2 cut(s)	65, 2449
BstMWI	GCNNNNNNNGC	6 cut(s)	238, 583, 776, 980, 1471, 2084
BstNI	CCWGG	3 cut(s)	294, 870, 2337
BstNSI	RCATGY	3 cut(s)	770, 914, 2400
BstPAI	GACNNNNGTC	1 cut(s)	1659
BstPI	GGTNACC	1 cut(s)	84
BstSCI	CCNGG	4 cut(s)	44, 292, 868, 2335
BstSFI	CTRYAG	2 cut(s)	1158, 1884
BstSLI	GKGCMC	1 cut(s)	1022
BstUI	CGCG	2 cut(s)	202, 402
BstV1I	GCAGC	5 cut(s)	710, 970, 992, 1172, 2213
BstV2I	GAAGAC	3 cut(s)	1449, 1764, 2139
BstX2I	RGATCY	1 cut(s)	1810
BstXI	CCANNNNNNTGG	1 cut(s)	631
BstYI	RGATCY	1 cut(s)	1810
BstZ17I	GTATAC	1 cut(s)	536
BsuRI	GGCC	8 cut(s)	118, 199, 232, 663, 815, 1020, 2236, 2426
BtgI	CCRYGG	3 cut(s)	200, 387, 2164
BtgZI	GCGATG	1 cut(s)	1178
BtrI	CACGTC	1 cut(s)	1490
BtsCI	GGATG	6 cut(s)	807, 816, 865, 996, 1072, 1316
BtsIMutI	CAGTG	4 cut(s)	755, 2094, 2237, 2416
BveI	ACCTGC	2 cut(s)	1133, 1229
CaiI	CAGNNNCTG	1 cut(s)	1559
Cfr10I	RCCGGY	2 cut(s)	24, 81
Cfr13I	GGNCC	8 cut(s)	141, 621, 661, 814, 872, 1018, 1019, 2055
Cfr42I	CCGCGG	1 cut(s)	203
Csp6I	GTAC	8 cut(s)	39, 487, 764, 1232, 2003, 2045, 2227, 2477
CspAI	ACCGGT	1 cut(s)	81
CviQI	GTAC	8 cut(s)	39, 487, 764, 1232, 2003, 2045, 2227, 2477
DdeI	CTNAG	5 cut(s)	93, 373, 393, 1087, 1371
DrdI	GACNNNNNNGTC	1 cut(s)	870
DseDI	GACNNNNNNGTC	1 cut(s)	870
EaeI	YGGCCR	1 cut(s)	197
Eam1104I	CTCTTC	3 cut(s)	9, 752, 843
EarI	CTCTTC	3 cut(s)	9, 752, 843
Eco130I	CCWWGG	1 cut(s)	2164
Eco24I	GRGCYC	1 cut(s)	1022
Eco47I	GGWCC	4 cut(s)	141, 621, 872, 2055
Eco57I	CTGAAG	2 cut(s)	143, 731
Eco88I	CYCGRG	4 cut(s)	221, 247, 349, 461
Eco91I	GGTNACC	1 cut(s)	84
EcoO109I	RGGNCCY	2 cut(s)	141, 1018
EcoO65I	GGTNACC	1 cut(s)	84
EcoRI	GAATTC	2 cut(s)	274, 2467
EcoRII	CCWGG	3 cut(s)	292, 868, 2335
EcoT14I	CCWWGG	1 cut(s)	2164
EcoT38I	GRGCYC	1 cut(s)	1022
ErhI	CCWWGG	1 cut(s)	2164
FaqI	GGGAC	3 cut(s)	876, 1647, 2373
FauI	CCCGC	4 cut(s)	195, 199, 575, 825
FbaI	TGATCA	2 cut(s)	361, 2211
FblI	GTMKAC	1 cut(s)	535
Fnu4HI	GCNGC	7 cut(s)	200, 699, 981, 984, 1161, 1325, 2202
FokI	GGATG	6 cut(s)	814, 823, 852, 983, 1079, 1323
FriOI	GRGCYC	1 cut(s)	1022
Fsp4HI	GCNGC	7 cut(s)	200, 699, 981, 984, 1161, 1325, 2202
GluI	GCNGC	7 cut(s)	200, 699, 981, 984, 1161, 1325, 2202
GsuI	CTGGAG	2 cut(s)	1699, 2292
HaeIII	GGCC	8 cut(s)	118, 199, 232, 663, 815, 1020, 2236, 2426
HapII	CCGG	5 cut(s)	25, 33, 46, 82, 1398
HincII	GTYRAC	1 cut(s)	740
HindII	GTYRAC	1 cut(s)	740
HpaII	CCGG	5 cut(s)	25, 33, 46, 82, 1398
Hpy188III	TCNNGA	9 cut(s)	351, 461, 934, 1652, 1776, 1901, 1913, 2270, 2309
Hpy99I	CGWCG	2 cut(s)	1474, 1882
HpyCH4III	ACNGT	7 cut(s)	187, 443, 1053, 1561, 1605, 2055, 2119
HpyCH4IV	ACGT	5 cut(s)	857, 930, 1489, 1543, 2154
HpyF10VI	GCNNNNNNNGC	6 cut(s)	238, 583, 776, 980, 1471, 2084
HpyF3I	CTNAG	5 cut(s)	93, 373, 393, 1087, 1371
HpySE526I	ACGT	5 cut(s)	857, 930, 1489, 1543, 2154
KpnI	GGTACC	1 cut(s)	2006
Ksp22I	TGATCA	2 cut(s)	361, 2211
KspI	CCGCGG	1 cut(s)	203
LmnI	GCTCC	4 cut(s)	1588, 1679, 1966, 2311
Lsp1109I	GCAGC	5 cut(s)	710, 970, 992, 1172, 2213
LweI	GCATC	6 cut(s)	373, 667, 1057, 1102, 1493, 2074
MaeII	ACGT	5 cut(s)	857, 930, 1489, 1543, 2154
MaeIII	GTNAC	8 cut(s)	84, 709, 1360, 1490, 1549, 1599, 1605, 2159
MfeI	CAATTG	1 cut(s)	1747
MflI	RGATCY	1 cut(s)	1810
MhlI	GDGCHC	1 cut(s)	1022
MlyI	GAGTC	5 cut(s)	876, 881, 1077, 1439, 2355
MmeI	TCCRAC	4 cut(s)	1035, 1101, 1734, 2417
MroXI	GAANNNNTTC	3 cut(s)	40, 567, 1221
MseI	TTAA	7 cut(s)	189, 312, 797, 1818, 1824, 2522, 2556
MslI	CAYNNNNRTG	2 cut(s)	431, 959
MspA1I	CMGCKG	3 cut(s)	124, 202, 983
MspI	CCGG	5 cut(s)	25, 33, 46, 82, 1398
MspR9I	CCNGG	4 cut(s)	46, 294, 870, 2337
MunI	CAATTG	1 cut(s)	1747
Mva1269I	GAATGC	2 cut(s)	350, 820
MvaI	CCWGG	3 cut(s)	294, 870, 2337
MvnI	CGCG	2 cut(s)	202, 402
MwoI	GCNNNNNNNGC	6 cut(s)	238, 583, 776, 980, 1471, 2084
NciI	CCSGG	1 cut(s)	46
NcoI	CCATGG	1 cut(s)	2164
NlaIV	GGNNCC	4 cut(s)	816, 849, 1020, 2004
NmuCI	GTSAC	5 cut(s)	84, 709, 1360, 1490, 2159
NspI	RCATGY	3 cut(s)	770, 914, 2400
NspV	TTCGAA	3 cut(s)	278, 571, 1215
OliI	CACNNNNGTG	1 cut(s)	431
PaeR7I	CTCGAG	1 cut(s)	349
PaqCI	CACCTGC	2 cut(s)	1133, 1229
PciI	ACATGT	1 cut(s)	2396
PcsI	WCGNNNNNNNCGW	1 cut(s)	2201
PctI	GAATGC	2 cut(s)	350, 820
PdmI	GAANNNNTTC	3 cut(s)	40, 567, 1221
PfeI	GAWTC	8 cut(s)	1097, 1277, 1406, 1511, 1634, 1802, 1904, 2038
PfoI	TCCNGGA	1 cut(s)	44
PinAI	ACCGGT	1 cut(s)	81
PkrI	GCNGC	7 cut(s)	201, 700, 982, 985, 1162, 1326, 2203
Ple19I	CGATCG	1 cut(s)	2449
PleI	GAGTC	5 cut(s)	876, 881, 1077, 1439, 2355
PpsI	GAGTC	5 cut(s)	876, 881, 1077, 1439, 2355
Ppu21I	YACGTR	1 cut(s)	1544
PpuMI	RGGWCCY	1 cut(s)	141
PscI	ACATGT	1 cut(s)	2396
PshAI	GACNNNNGTC	1 cut(s)	1659
PshBI	ATTAAT	1 cut(s)	2556
Psp1406I	AACGTT	1 cut(s)	2154
Psp5II	RGGWCCY	1 cut(s)	141
Psp6I	CCWGG	3 cut(s)	292, 868, 2335
PspEI	GGTNACC	1 cut(s)	84
PspGI	CCWGG	3 cut(s)	292, 868, 2335
PspN4I	GGNNCC	4 cut(s)	816, 849, 1020, 2004
PspOMI	GGGCCC	1 cut(s)	1018
PspPI	GGNCC	8 cut(s)	141, 621, 661, 814, 872, 1018, 1019, 2055
PspPPI	RGGWCCY	1 cut(s)	141
PstI	CTGCAG	1 cut(s)	1162
PstNI	CAGNNNCTG	1 cut(s)	1559
PsuI	RGATCY	1 cut(s)	1810
PvuI	CGATCG	1 cut(s)	2449
PvuII	CAGCTG	2 cut(s)	124, 983
RsaI	GTAC	8 cut(s)	40, 488, 765, 1233, 2004, 2046, 2228, 2478
RsaNI	GTAC	8 cut(s)	39, 487, 764, 1232, 2003, 2045, 2227, 2477
RseI	CAYNNNNRTG	2 cut(s)	431, 959
SacII	CCGCGG	1 cut(s)	203
SaqAI	TTAA	7 cut(s)	189, 312, 797, 1818, 1824, 2522, 2556
SatI	GCNGC	7 cut(s)	200, 699, 981, 984, 1161, 1325, 2202
Sau96I	GGNCC	8 cut(s)	141, 621, 661, 814, 872, 1018, 1019, 2055
ScaI	AGTACT	1 cut(s)	40
SchI	GAGTC	5 cut(s)	876, 881, 1077, 1439, 2355
ScrFI	CCNGG	4 cut(s)	46, 294, 870, 2337
SduI	GDGCHC	1 cut(s)	1022
SfaNI	GCATC	6 cut(s)	373, 667, 1057, 1102, 1493, 2074
SfcI	CTRYAG	2 cut(s)	1158, 1884
Sfr274I	CTCGAG	1 cut(s)	349
Sfr303I	CCGCGG	1 cut(s)	203
SfuI	TTCGAA	3 cut(s)	278, 571, 1215
SgrBI	CCGCGG	1 cut(s)	203
SinI	GGWCC	4 cut(s)	141, 621, 872, 2055
SlaI	CTCGAG	1 cut(s)	349
SmiMI	CAYNNNNRTG	2 cut(s)	431, 959
SmlI	CTYRAG	1 cut(s)	349
SmoI	CTYRAG	1 cut(s)	349
StyD4I	CCNGG	4 cut(s)	44, 292, 868, 2335
StyI	CCWWGG	1 cut(s)	2164
TaaI	ACNGT	7 cut(s)	187, 443, 1053, 1561, 1605, 2055, 2119
TaiI	ACGT	5 cut(s)	860, 933, 1492, 1546, 2157
TaqII	GACCGA	2 cut(s)	609, 1607
TatI	WGTACW	3 cut(s)	38, 486, 2476
TauI	GCSGC	2 cut(s)	202, 1327
TfiI	GAWTC	8 cut(s)	1097, 1277, 1406, 1511, 1634, 1802, 1904, 2038
Tru1I	TTAA	7 cut(s)	189, 312, 797, 1818, 1824, 2522, 2556
Tru9I	TTAA	7 cut(s)	189, 312, 797, 1818, 1824, 2522, 2556
TscAI	CASTG	4 cut(s)	755, 2101, 2237, 2423
TseFI	GTSAC	5 cut(s)	84, 709, 1360, 1490, 2159
TseI	GCWGC	5 cut(s)	698, 980, 983, 1160, 2201
Tsp45I	GTSAC	5 cut(s)	84, 709, 1360, 1490, 2159
TspGWI	ACGGA	2 cut(s)	1095, 1438
TspRI	CASTG	4 cut(s)	755, 2101, 2237, 2423
VpaK11BI	GGWCC	4 cut(s)	141, 621, 872, 2055
VspI	ATTAAT	1 cut(s)	2556
XapI	RAATTY	4 cut(s)	274, 787, 1218, 2467
XceI	RCATGY	3 cut(s)	770, 914, 2400
XhoI	CTCGAG	1 cut(s)	349
XmiI	GTMKAC	1 cut(s)	535
XmnI	GAANNNNTTC	3 cut(s)	40, 567, 1221
ZrmI	AGTACT	1 cut(s)	40

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.